Academic literature on the topic 'Lignocellulose pretreatments'
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Journal articles on the topic "Lignocellulose pretreatments"
Naini, Al-Arofatus, Nurwahdah Nurwahdah, Ratri Yuli Lestari, and Sunardi Sunardi, Ph.D. "Praperlakuan secara Hidrotermal Limbah Lignoselulosa untuk Produksi Bioetanol Generasi Kedua (Pretreatment of Lignocellulose Wastes Using Hydrothermal Method for Producing Second Generation Bioethanol)." Jurnal Riset Industri Hasil Hutan 10, no. 2 (December 28, 2018): 93–102. http://dx.doi.org/10.24111/jrihh.v10i2.4078.
Full textZahoor, Wen Wang, Xuesong Tan, Qiang Yu, Yongming Sun, Zhenhong Yuan, Kyoungseon Min, Jinsuk Lee, Zi Shang Bai, and Xinshu Zhuang. "Comparison of Low-Temperature Alkali/Urea Pretreatments for Ethanol Production from Wheat Straw." Journal of Biobased Materials and Bioenergy 15, no. 3 (June 1, 2021): 399–407. http://dx.doi.org/10.1166/jbmb.2021.2062.
Full textLi, Ao, Qiaomei Yang, Yu Li, Shiguang Zhou, Jiangfeng Huang, Meng Hu, Yuanyuan Tu, Bo Hao, Liangcai Peng, and Tao Xia. "Mild physical and chemical pretreatments to enhance biomass enzymatic saccharification and bioethanol production from Erianthus arundinaceus." BioResources 14, no. 1 (December 3, 2018): 650–68. http://dx.doi.org/10.15376/biores.14.1.650-668.
Full textOates, Nicola C., Amira Abood, Alexandra M. Schirmacher, Anna M. Alessi, Susannah M. Bird, Joseph P. Bennett, Daniel R. Leadbeater, et al. "A multi-omics approach to lignocellulolytic enzyme discovery reveals a new ligninase activity from Parascedosporium putredinis NO1." Proceedings of the National Academy of Sciences 118, no. 18 (April 26, 2021): e2008888118. http://dx.doi.org/10.1073/pnas.2008888118.
Full textCosta, Stefania, Irene Rugiero, Christian Larenas Uria, Paola Pedrini, and Elena Tamburini. "Lignin Degradation Efficiency of Chemical Pre-Treatments on Banana Rachis Destined to Bioethanol Production." Biomolecules 8, no. 4 (November 9, 2018): 141. http://dx.doi.org/10.3390/biom8040141.
Full textHuang, Caoxing, Ruolin Li, Wei Tang, Yayue Zheng, and Xianzhi Meng. "Improve Enzymatic Hydrolysis of Lignocellulosic Biomass by Modifying Lignin Structure via Sulfite Pretreatment and Using Lignin Blockers." Fermentation 8, no. 10 (October 20, 2022): 558. http://dx.doi.org/10.3390/fermentation8100558.
Full textPérez-Merchán, Antonio Manuel, Gabriela Rodríguez-Carballo, Benjamín Torres-Olea, Cristina García-Sancho, Pedro Jesús Maireles-Torres, Josefa Mérida-Robles, and Ramón Moreno-Tost. "Recent Advances in Mechanochemical Pretreatment of Lignocellulosic Biomass." Energies 15, no. 16 (August 17, 2022): 5948. http://dx.doi.org/10.3390/en15165948.
Full textMahmood, Hamayoun, Saqib Mehmood, Ahmad Shakeel, Tanveer Iqbal, Mohsin Ali Kazmi, Abdul Rehman Khurram, and Muhammad Moniruzzaman. "Glycerol Assisted Pretreatment of Lignocellulose Wheat Straw Materials as a Promising Approach for Fabrication of Sustainable Fibrous Filler for Biocomposites." Polymers 13, no. 3 (January 26, 2021): 388. http://dx.doi.org/10.3390/polym13030388.
Full textYang, Haiyan, Yuanchen Zhu, Yan Jin, Fuhou Lei, Zhengjun Shi, and Jing Yang. "Pseudo-lignin retarded bioconversion of sugarcane bagasse holocellulose after liquid hot water and acid pretreatments." BioResources 16, no. 2 (April 22, 2021): 4052–63. http://dx.doi.org/10.15376/biores.16.2.4052-4063.
Full textValdés, Gabriela, Regis Teixeira Mendonça, and George Aggelis. "Lignocellulosic Biomass as a Substrate for Oleaginous Microorganisms: A Review." Applied Sciences 10, no. 21 (October 30, 2020): 7698. http://dx.doi.org/10.3390/app10217698.
Full textDissertations / Theses on the topic "Lignocellulose pretreatments"
Munns, Craig Christopher Robert. "Development of physio-chemical pretreatments and mixed microbial cultures for the conversion of lignocellulosic biomass to useful products." Thesis, University of Edinburgh, 2017. http://hdl.handle.net/1842/28768.
Full textBadalato, Nelly. "Structure de déchets lignocellulosiques : effets sur la colonisation, les communautés microbienne et les performances de méthanisation, caractérisés par des approches fonctionnelles et haut-débit." Electronic Thesis or Diss., Paris, AgroParisTech, 2014. http://www.theses.fr/2014AGPT0002.
Full textLignocellulosic materials have a high energy potential and are abundant, especially in municipal solid waste and their methanization is a promising waste-to-energy bioprocess. However, owing to their highly complex and heterogeneous structure, they are recalcitrant to anaerobic conditions and the use of pre-treatments is usually required to improve their biodegradation yields. Besides, lignocellulose colonization by cellulolytic microorganisms is a key step for an efficient biodegradation. In this context, the PhD work aimed to better understand the factors affecting waste colonization, to establish the link between lignocellulosic waste colonization and its biodegradation efficiency and to characterize more precisely the mechanisms and interactions within the biomass. A transversal approach was developed, combining cultures of model pure strains and lab-scale methanization microcosms with a complex biomass. Integrated approaches were applied to these studies, combining high-throughput analyses (metagenomics/(meta) proteomics), physico-chemical monitoring of bioconversion and finally physico-chemical characterization of substrates. The main results highlight the important role of lignocellulosic materials chemical and micro-and macro -structural features for their recalcitrance, their biodegradation efficiency and the response of the microbial compartment. The first global quantitative proteomic study on the cellulolytic model Clostridium cellulolyticum was conducted. Results showed an increased biodegradation rate of the facial tissue compared to cotton. This enhanced biodegradation was associated to a particular metabolic profile, a faster and more extensive colonization and finally a quantitative modulation of the cellulasic system. On the other hand, study of lignocellulosic waste methanization confirmed the good agreement between this more realistic system and the above-described model system. It also provided new information about the effects of substrate on microbial community structure. Noticeably, Bacteroidia members predominated in the presence of tissue and a high proportion of Spirochaetes members was observed in the presence of cotton. Finally, study of the effects of wheat straw and cardboard dry grinding revealed the limitations of these pretreatments on biodegradation efficiency. Main key points were a moderate positive effect of wheat straw fine grinding, and the sensitivity of the microbial communities to substrate surface characteristics, as evidenced by the emergence of different microbial communities according to the applied mechanical pretreatment. In conclusion, this work brings new perspectives to the study of lignocellulosic waste recalcitrance by addressing both the structural, functional and ecological aspects. These results contribute to the core fundamental knowledge on bioprocesses. They confirm that the lignocellulosic materials are specific among non-hazardous waste and require the implementation of adapted specific processes
Monlau, Florian. "Application of pretreatments to enhance biohydrogen and/or biomethane from lignocellulosic residues : linking performances to compositional and structural features." Thesis, Montpellier 2, 2012. http://www.theses.fr/2012MON20178/document.
Full textIn the future, various forms of renewable energy, such as second generation biofuels from lignocellulosic residues, will be required to replace fossil fuels. Among these, biohydrogen and methane produced through fermentative processes appear as interesting candidates. However, biohydrogen and/or methane production of lignocellulosic residues is often limited by the recalcitrant structure and a pretreatment step prior to fermentative processes is often required. Up to date, informations on lignocellulosic characteristics limiting both hydrogen and methane production are limited.Therefore, this work aims to investigate the effect of compositional and structural features of lignocellulosic residues on biohydrogen and methane performances for further developping appropriate pretreatments strategies. Firstly, a panel of twenty lignocellulosic residues was used to correlate both hydrogen and methane potentials with the compositional and structural characteristics. The results showed that hydrogen potential positively correlated with soluble carbohydrates only. Secondly, methane potential correlated negatively with lignin content and, in a lesser extent, with crystalline cellulose, but positively with the soluble carbohydrates, amorphous holocelluloses and protein contents. Pretreatments strategies were further developed to enhance both hydrogen and methane production of sunflower stalks. Dilute-acid and combined alkaline-enzymatic pretreatments, which were found efficient in solubilizing holocelluloses into soluble carbohydrates, were applied prior to biohydrogen potential tests. By combined alkaline-enzymatic pretreatment, hydrogen potential was fifteen times more than that of untreated samples. On the contrary, hydrogen production was inhibited after dilute-acid pretreatments due to the release of byproducts (furfural, 5-HMF and phenolic compounds) that led to microbial communities shift toward no hydrogen producing bacteria. Similarly, methane production, five thermo-chemical pretreatments (NaOH, H2O2, Ca(OH)2, HCl and FeCl3) found efficient in delignification or solubilization of holocelluloses, were considered. Among these pretreatments, the best conditions were 55°C with 4% NaOH for 24 h and led to an increase of 29-44 % in methane potential of sunflower stalks. This pretreatment condition was validated in one stage anaerobic mesophilic continuous digester for methane production and was found efficient to enhance from 26.5% the total energy produced compared to one stage-CH4 alone. Two-stage H2 (batch) / CH4 (continuous) process was also investigated. Nevertheless, in term of energy produced, no significant differences were observed between one-stage CH4 and two-stage H2 /CH4
Cheng, Wei. "Pretreatment and enzymatic hydrolysis of lignocellulosic materials." Morgantown, W. Va. : [West Virginia University Libraries], 2001. http://etd.wvu.edu/templates/showETD.cfm?recnum=1951.
Full textTitle from document title page. Document formatted into pages; contains xii, 173 p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 138-142).
Warsame, Mohamed. "Saccharification of lignocellulose." Thesis, Malmö högskola, Fakulteten för hälsa och samhälle (HS), 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-25910.
Full textThe increasing energy demand and the anticipated decline in crude oil production has led to an immense search for new energy sources. Plant cell walls contain lignocellulose that conserve great amounts of energy. These polysaccharides are of high importance for the search of renewable energy sources. Pretreatment of the cell wall is necessary in order to hydrolyse it to its component sugars. Once degraded to monomeric sugars it can be fermented to either ethanol or biogas through established fermentation technologies.The aim of this thesis was to compare and evaluate some of the methods used for sacchrification of lignocellulose. Three treatments where compared to determine which is highest yielding. These are enzymatic hydrolysis, microwave irradiation and steam explosion.Wheat straw was used as substrate and hydrolysed by three commercial enzyme mixtures. Samples were pretreated before the enzymatic reaction with either microwave or steam explosion. Results showed that a treatment of either microwave irradiation or steam explosion combined with enzyme hydrolysis gives the highest yield in monomeric sugars. The conclusions that can be drawn are that mechanical pretreatment increases yield drastically but is insufficient in its self. Further enzymatic treatment of wheat straw is necessary to obtain high amounts of simple sugars.
Brandt, Agnieszka. "Ionic liquid pretreatment of lignocellulosic biomass." Thesis, Imperial College London, 2012. http://hdl.handle.net/10044/1/9166.
Full textCorredor, Deisy Y. "Pretreatment and enzymatic hydrolysis of lignocellulosic biomass." Diss., Manhattan, Kan. : Kansas State University, 2008. http://hdl.handle.net/2097/693.
Full textKvillborn, Carin. "Enzymatic Pretreatment of Lignocellulose Rich Waste for Improved Biogas Production." Thesis, Linköpings universitet, Tema vatten i natur och samhälle, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-104974.
Full textStudien avsåg att undersöka metanutbytet från anaerob nedbrytning med förbehandlad lignocellulosa som substrat. Lignocellulosamaterialet, i form av skogsavfall, maldes och förbehandlades därefter med det organiska lösningsmedlet NMMO (N-metylmorfolin-N-oxid) och/eller de lignolytiska enzymerna laccase och versatile peroxidas med dosen 60 U g-1 torrsubstanshalt (TS). Mängden producerad metan undersöktes i en biometanpotentialanalys med inocula från en termofil biogasreaktor, som behandlade hushållsavfall. Triplikat av varje prov användes för att öka den statistiska stabiliteten. På grund av det stora antalet prover genomfördes studien i två omgångar: Serie 10 & 20 samt serie 30 & 40. Resultaten visade att det NMMO-behandlade skogsavfallet gav 130 NmL CH4 g-1 organisk substans (VS) och det obehandlade skogsavfallet gav 95 NmL CH4 g-1 VS i serie 10 & 20. Både obehandlat och NMMO- behandlat skogsavfall gav 140 NmL CH4 g-1 VS i serie 30 & 40. Förbehandling med NMMO verkar vara fördelaktig medan enzymbehandling endast resulterade i en smärre ökning av gasproduktionen. En analys av vätskan efter enzymbehandlingen visade förekomst av fenoler, vilket visar på en lyckad ligninnedbrytning.
Narayana, Swamy Naveen. "Supercritical Carbon Dioxide Pretreatment of Various Lignocellulosic Biomasses." Ohio University / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1269524607.
Full textMoxley, Geoffrey W. "Studies of Cellulosic Ethanol Production from Lignocellulose." Thesis, Virginia Tech, 2007. http://hdl.handle.net/10919/43372.
Full textLignocellulose materials are structurally composed of five types of polymeric sugars, glucan, galactan, mannan, arabinan, and xylan. NREL has developed a quantitative saccharification (QS) method for determining carbohydrate composition. We proposed a new protocol based on the NREL 2006 Laboratory Analytical Procedure â Determination of Structural Carbohydrates and Lignin in Biomassâ (Sluiter et al. 2006a) with a slight modification, in which xylose concentration was determined after the secondary hydrolysis by using 1% sulfuric acid rather than 4% sulfuric acid. We found that the current NREL protocol led to a statistically significant overestimation of acid-labile xylan content ranging from 4 to 8 percent.
Lignocellulosic biomass is naturally recalcitrant to enzymatic hydrolysis, and must be pretreated before it can be effectively used for bioethanol production. One such pretreatment is a fractionation process that separates lignin and hemicellulose from the cellulose and converts crystalline cellulose microfibrils to amorphous cellulose. Here we evaluated the feasibility of lignocellulose fractionation applicable to the hurds of industrial hemp. Hurds are the remaining material of the stalk after all leaves, seeds, and fiber have been stripped from the plant. After optimizing acid concentration, reaction time and temperature, the pretreated cellulosic samples were hydrolyzed to more than 96% after 24 hours of hydrolysis (enzyme loading conditions of 15 FPU/g glucan Spezyme CP and 60 IU/g glucan Novozyme 188) at the optimal pretreatment condition (> 84% H3PO4, > 50 °C and > 1 hour). The overall glucose and xylose yields were 89% (94% pretreatment; 96% digestibility) and 61%, respectively. All data suggest the technical feasibility of building a biorefinery based on the hurds of industrial hemp as a feedstock and a new lignocellulose fractionation technology for producing cellulosic ethanol. The choice of feedstock and processing technology gives high sugar yields, low processing costs, low cost feedstock, and low capital investment.
Master of Science
Books on the topic "Lignocellulose pretreatments"
Bajpai, Pratima. Pretreatment of Lignocellulosic Biomass for Biofuel Production. Singapore: Springer Singapore, 2016. http://dx.doi.org/10.1007/978-981-10-0687-6.
Full textBajpai, Pratima. Deep Eutectic Solvents for Pretreatment of Lignocellulosic Biomass. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-4013-1.
Full textHumbird, David. Process design and economics for biochemical conversion of lignocellulosic biomass to ethanol: Dilute-acid pretreatment and enzymatic hydrolysis of corn stover. Golden, CO: National Renewable Energy Laboratory, 2011.
Find full textBajpai, Pratima. Pretreatment of Lignocellulosic Biomass for Biofuel Production. Springer London, Limited, 2016.
Find full textBajpai, Pratima. Pretreatment of Lignocellulosic Biomass for Biofuel Production. Springer, 2016.
Find full textBajpai, Pratima. Deep Eutectic Solvents for Pretreatment of Lignocellulosic Biomass. Springer Singapore Pte. Limited, 2021.
Find full textBook chapters on the topic "Lignocellulose pretreatments"
Shafiei, Marzieh, Rajeev Kumar, and Keikhosro Karimi. "Pretreatment of Lignocellulosic Biomass." In Lignocellulose-Based Bioproducts, 85–154. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-14033-9_3.
Full textTakara, Devin, Prachand Shrestha, and Samir Kumar Khanal. "Lignocellulosic Biomass Pretreatment." In Bioenergy and Biofuel from Biowastes and Biomass, 172–200. Reston, VA: American Society of Civil Engineers, 2010. http://dx.doi.org/10.1061/9780784410899.ch09.
Full textChen, Hongzhang. "Pretreatment and Primary Refining of Lignocelluloses." In Biotechnology of Lignocellulose, 143–85. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-007-6898-7_4.
Full textRoy, Shyamal. "Physicochemical Pretreatments." In Pre-Treatment Methods of Lignocellulosic Biomass for Biofuel Production, 13–22. Boca Raton: CRC Press, 2021. http://dx.doi.org/10.1201/9781003203414-3.
Full textRoy, Shyamal. "Physical Pretreatments." In Pre-Treatment Methods of Lignocellulosic Biomass for Biofuel Production, 6–12. Boca Raton: CRC Press, 2021. http://dx.doi.org/10.1201/9781003203414-2.
Full textRoy, Shyamal. "Chemical Pretreatments." In Pre-Treatment Methods of Lignocellulosic Biomass for Biofuel Production, 23–44. Boca Raton: CRC Press, 2021. http://dx.doi.org/10.1201/9781003203414-4.
Full textWoiciechowski, Adenise Lorenci, Luciana Porto de Souza Vandenberghe, Susan Grace Karp, Luiz Alberto Junior Letti, Júlio Cesar de Carvalho, Adriane Bianchi Pedroni Medeiros, Michele Rigon Spier, Vincenza Faraco, Vanete Thomaz Soccol, and Carlos Ricardo Soccol. "The Pretreatment Step in Lignocellulosic Biomass Conversion: Current Systems and New Biological Systems." In Lignocellulose Conversion, 39–64. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-37861-4_3.
Full textKim, Tae Hyun. "Pretreatment of Lignocellulosic Biomass." In Bioprocessing Technologies in Biorefinery for Sustainable Production of Fuels, Chemicals, and Polymers, 91–110. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2013. http://dx.doi.org/10.1002/9781118642047.ch6.
Full textBajpai, Pratima. "Pretreatment of Lignocellulosic Biomass." In SpringerBriefs in Molecular Science, 17–70. Singapore: Springer Singapore, 2016. http://dx.doi.org/10.1007/978-981-10-0687-6_4.
Full textMcMillan, James D. "Pretreatment of Lignocellulosic Biomass." In ACS Symposium Series, 292–324. Washington, DC: American Chemical Society, 1994. http://dx.doi.org/10.1021/bk-1994-0566.ch015.
Full textConference papers on the topic "Lignocellulose pretreatments"
BICHOT, Aurélie, Jean Philippe DELGENES, Marilena RADOIU, and Diana GARCIA BERNET. "MICROWAVE PRETREATMENT OF LIGNOCELLULOSIC BIOMASS TO RELEASE MAXIMUM PHENOLIC ACIDS." In Ampere 2019. Valencia: Universitat Politècnica de València, 2019. http://dx.doi.org/10.4995/ampere2019.2019.9629.
Full textNikolić, Valentina, Slađana Žilić, Danka Milovanović, Beka Sarić, and Marko Vasić. "NOVEL TRENDS IN APPLICATION AND PRETREATMENT OF LIGNOCELLULOSIC AGRICULTURAL WASTE." In 1st International Symposium on Biotechnology. University of Kragujevac, Faculty of Agronomy, 2023. http://dx.doi.org/10.46793/sbt28.271n.
Full textWeitao Zhang, Minliang Yang, and Kurt A. Rosentrater. "Pretreatment Methods for Lignocellulosic Biomass to Ethanol." In 2013 Kansas City, Missouri, July 21 - July 24, 2013. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2013. http://dx.doi.org/10.13031/aim.20131594712.
Full textToma, Magdalena-Laura, Gheorghe Voicu, Mariana Ferdes, and Mirela-Nicoleta Dinca. "Preliminary research in microorganism pretreatment of biomass for lignocellulose degradation." In 17th International Scientific Conference Engineering for Rural Development. Latvia University of Agriculture, 2018. http://dx.doi.org/10.22616/erdev2018.17.n422.
Full textChatteriee, Sayan, Abhirup Ghosh, Talha Khan, Soumyadip Roy, Anustup Chatterjee, and Mainak Biswas. "Modeling of Pretreatment Process of Lignocellulosic Biomass by Dilute Acid Hydrolysis." In 2019 3rd International Conference on Electronics, Materials Engineering & Nano-Technology (IEMENTech). IEEE, 2019. http://dx.doi.org/10.1109/iementech48150.2019.8981124.
Full textGuo, Wanqian, Ze He, and Jing Li. "The Development Of Hydrogen Production From Lignocellulosic Biomass: Pretreatment And Process." In 2016 International Conference on Advances in Energy, Environment and Chemical Science. Paris, France: Atlantis Press, 2016. http://dx.doi.org/10.2991/aeecs-16.2016.49.
Full textMutrakulcharoen, Parita, Malinee Sriariyanun, Wasinee Pongprayoon, Theerawut Phusantisampan, and Supacharee Roddecha. "Recycling of 1-ethyl-3-methylimidazolium acetate in lignocellulosic biomass pretreatment." In the 8th International Conference. New York, New York, USA: ACM Press, 2019. http://dx.doi.org/10.1145/3323716.3323723.
Full textJiele Xu, Ximing Zhang, Pankaj Pandey, and Jay J Cheng. "Pretreatment of Lignocellulosic Biomass with Recycled Black Liquor for Sugar Production." In 2012 Dallas, Texas, July 29 - August 1, 2012. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2012. http://dx.doi.org/10.13031/2013.41807.
Full textLuo, Shaohua, Jinghua Cheng, Ruming Zhao, and Dachun Gong. "Evaluation of enzymatic hydrolysis on the process of wet explosion pretreatment for the lignocellulose." In 2011 International Conference on Electrical and Control Engineering (ICECE). IEEE, 2011. http://dx.doi.org/10.1109/iceceng.2011.6058347.
Full textARASTEH, ALI, and RASOOL GHASEMZADEH. "Biological pretreatment of lignocellulosic materials with white rot fungi for enzymatic hydrolysis." In Fourth International Conference on Advances in Bio-Informatics and Environmental Engineering - ICABEE 2016. Institute of Research Engineers and Doctors, 2016. http://dx.doi.org/10.15224/978-1-63248-100-9-14.
Full textReports on the topic "Lignocellulose pretreatments"
Zhu, Junyong, Chao Zhang, Roland Gleisner, Carl Houtman, and Xuejun Pan. Bioconversion of woody biomass to biofuel and lignin co-product using sulfite pretreatment to overcome the recalcitrance of lignocelluloses (SPORL). Madison, WI: U.S. Department of Agriculture, Forest Service, Forest Products Laboratory, 2016. http://dx.doi.org/10.2737/fpl-gtr-240.
Full textHumbird, D., R. Davis, L. Tao, C. Kinchin, D. Hsu, A. Aden, P. Schoen, et al. Process Design and Economics for Biochemical Conversion of Lignocellulosic Biomass to Ethanol: Dilute-Acid Pretreatment and Enzymatic Hydrolysis of Corn Stover. Office of Scientific and Technical Information (OSTI), March 2011. http://dx.doi.org/10.2172/1013269.
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