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1
Johnson, Casey Ray. "Prebiotics in Lentil (Lens Culinaris L.)." Thesis, North Dakota State University, 2013. https://hdl.handle.net/10365/27035.
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Prebiotic carbohydrates are an important component of healthy diet. The objectives of this study were (1) to compare the concentrations of prebiotic carbohydrates in different lentil genotypes and growing locations and (2) to compare the concentrations of prebiotic carbohydrates in lentil under various processing and preparation procedures. Mean concentrations of prebiotics in lentil genotypes were as follows: raffinose-family oligosaccharides (RFO) (raffinose + stachyose, 2509 mg; verbascose, 1562 mg), fructo-oligosaccharides (nystose, 62 mg), sugar alcohols (sorbitol, 1220 mg; mannitol, 203 mg), and resistant starch (RS), 7.5 g 100 g-1. Modest RFO concentration reductions were observed with cooking, cooling, and reheating. Mean RS concentration in raw, cooked, cooled, and reheated lentil were 3.0, 3.0, 5.1, and 5.1 g/100g respectively, clearly demonstrating cooling-induced synthesis of RS. Study results suggest that lentil contains nutritionally significant concentrations of prebiotics and that those concentrations may be enhanced through breeding, locational sourcing, and cooking and preparation procedures.North Dakota State University Experimental Station
Northern Pulse Growers Association
USA Dry Pea and Lentil Council
2
Khorramdelazad, Mahsa. "Investigation of genetic and molecular mechanisms of lentil (Lens culinaris) cv. ILL7537 during early defence responses to Ascochyta lentis." Thesis, Griffith University, 2019. http://hdl.handle.net/10072/389670.
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Lentil (Lens culinaris) is a valuable and popular cool season legume worldwide. The lens shaped seed legume is a rich source of protein (up to 35%), complex carbohydrates, vitamin A and B, and potassium and iron and is high in fibre and low in sodium and fat. Therefore, lentil is a staple food in no- and low-meat diets especially in North Africa, the Middle East, the Indian subcontinent and parts of the Americas. Due to the high demand for lentil globally, its annual production has increased from 0.85 to 5.03 Mt within the last five decades. Australia is the fourth largest producer in the world with an annual export value of $185 million AUD. However, lentil global production and yield quality is greatly affected by the devastating fungal disease “Ascochyta blight” (AB).
Ascochyta lentis (Vassilievsky) is a necrotrophic fungus that causes AB, affecting all above-ground parts of the plant resulting in up to 70% yield loss and marketability reduction annually. The fungus is endemic to lentil growing regions globally and is the top biotic constraint to lentil production in Australia. A range of studies have been conducted on A. lentis to clarify the epidemiology, diagnostics, lifecycle, survival, chemical susceptibility as well as pathogenic variation and physiology of the host-pathogen interaction. The knowledge on A. lentis pathogenicity together with identification of genetic and molecular mechanisms of lentil defence in the naturally resistant lentil genotypes would lead to the most economic, environmentally friendly and effective method of disease management.
This study identified the genetic and molecular aspects of defence to A. lentis by identifying novel defence related genes and molecular pathways, their functions and locations on lentils chromosomes as well as some linked SNPs to the candidate genes. The identified genes, SNPs and QTLs identified may be used as genetic tools for the selection of A. lentis resistance within ILL7537 in future pre-breeding efforts.Thesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Environment and Sc
Science, Environment, Engineering and Technology
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3
El-Hassan, Said A. K. "Biological control of vascular wilt of lentil (Fusarium oxysporum f. sp. lentis) by Bacillus subtilis and Trichoderma hamatum." Thesis, University of Reading, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.405539.
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4
Ferreira, Daniele Alves. "Reactions using whole cell biocalytic lens culinaris (lentil)." Universidade Federal do CearÃ, 2012. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=8566.
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CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel SuperiorThis paper describes the use of seeds of lentil (Lens culinaris) as intact plant system in biocatalytic reactions of reduction and hydrolysis. Initially, some plant sources were evaluated for their action in the biocatalytic reduction of aromatic ketone acetophenone. The plants that showed positive results in the reduction reaction underwent determination of their protein content by the methods of Lowry and Bradford. Lens culinaris was plant species revealed that most relevant biocatalytic activity, in agreement with their high protein content (1.33 mg / mL and 1.07 mg / mL according to Bradford and Lowry methods, respectively), and thus The plant was selected for further study. Subsequently, always using the model substrate acetophenone as the reaction parameters were optimized: concentration of biocatalyst; reaction kinetics; stirring speed the reaction medium. After selecting these parameters, the bioreduction reactions were extended to other derivatives of prochiral acetophenone, which were observed with bioconversions yields and enantiomeric excess (ee) ranging from low to high percentages. Generally, were observed steric and electronic influences due to the type and relative position of the various substituents on aromatic compounds. In the case of substrates containing two different functional groups susceptible to reduction, low chemoselectivity observed. Investigated also other carbonyl compounds such as aliphatic and aromatic ketones, aromatic aldehydes and aromatic nitro compounds. In addition investigated the possibility that hydrolytic action from compounds belonging functions esters, amides and nitriles. The yields of the reactions were calculated using Gas Chromatography coupled with Mass Spectrometry (GC-MS) and Nuclear Magnetic Resonance Hydrogen (1H NMR). The enantiomeric excess (ee) were determined by High Performance Liquid Chromatography (HPLC) equipped with chiral columns.
Este trabalho relata a utilizaÃÃo das sementes de lentilha (Lens culinaris) como sistema Ãntegro de planta em reaÃÃes biocatalÃticas de reduÃÃo e hidrÃlise. Inicialmente, algumas fontes vegetais foram avaliadas quanto sua aÃÃo biocatalÃtica na reduÃÃo da cetona aromÃtica acetofenona. Os vegetais que revelaram resultados positivos na reaÃÃo de reduÃÃo foram submetidos à determinaÃÃo do seu teor de proteÃnas atravÃs dos mÃtodos de Lowry e Bradford. Lens culinaris foi à espÃcie vegetal que revelou atividade biocatalÃtica mais relevante, em acordo com seu alto teor de proteÃna (1,33 mg/mL e 1,07 mg/mL, segundo os mÃtodos de Bradford e Lowry, respectivamente), e assim, foi o vegetal selecionado para estudos mais aprofundados. Posteriormente, utilizando sempre a acetofenona como substrato modelo foram otimizados os parÃmetros reacionais: concentraÃÃo do biocatalisador; cinÃtica de reaÃÃo; velocidade de agitaÃÃo e meio reacional. ApÃs seleÃÃo desses parÃmetros, as reaÃÃes de biorreduÃÃo foram estendidas a outros derivados prÃ-quirais da acetofenona, onde foram observadas bioconversÃes com rendimentos e excessos enantiomÃricos (ee) que variaram de baixas a elevadas porcentagens. De modo geral, foram observadas influencias eletrÃnica e estÃrica devidas ao tipo e a posiÃÃo relativa dos diferentes substituintes nos compostos aromÃticos. No caso de substratos contendo dois grupos funcionais diferentes suscetÃveis de reduÃÃo, foi observada baixa quimiosseletividade. Foram investigados tambÃm outros compostos carbonilicos, como: cetonas alifÃticas e aromÃticas, aldeÃdos aromÃticos e nitrocompostos aromÃticos. Em adiÃÃo foi investigado a possibilidade de aÃÃo hidrolÃtica a partir de compostos pertencente Ãs funÃÃes Ãsteres, amidas e nitrilas. Os rendimentos das reaÃÃes foram calculados utilizando Cromatografia Gasosa acoplada à Espectrometria de Massas (CG-EM) e RessonÃncia MagnÃtica Nuclear de HidrogÃnio (RMN 1H). Os excessos enantiomÃricos (ee) foram determinados atravÃs de Cromatografia LÃquido de Alta EficiÃncia (CLAE) equipada com colunas quirais.
5
Kemal, Seid Ahmed. "Pathogenic variability, inheritance of virulence and host-pathogen interaction with temperature in Ascochyta fabae f. sp. lentis on lentil." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1996. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq24061.pdf.
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6
Bakhsh, Ahmad. "Genetic analysis of seed characters in lentil (Lens Culinaris)." Thesis, University of East Anglia, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.334607.
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7
Ekanayake, Lukshman Jayampathi. "Selenium on Increasing Lentil (Lens Culinaris Medikus.) Grain Yield." Thesis, North Dakota State University, 2014. https://hdl.handle.net/10365/27405.
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Selenium is an essential element for mammals but it has not been considered as essential for higher plants. The effect of Se application on lentil grain yield, antioxidant activity, nitrogen fixation, and seed Se concentration studied. Experiments were conducted at Carrington Research and Extension Center in 2012 and 2013. Selenium fertilization increased the lentil grain yield by 5 % in 2012 and by 9 % in 2013. Selenium application increased the antioxidant activity (70 % inhibition) of lentils compared to the untreated control (61% inhibition). Percentage nitrogen derived from air was increased with Se fertilization by 17 % and 12 % in 2012 and 2013, respectively, with Se fertilization. Seed Se concentration was significantly higher in selenate treated lentils (1.4 mg/kg) compared to selenite (0.9 mg/kg) and the control (0.6 mg/kg). Overall, application of Se increased lentil grain yield, nitrogen fixation, antioxidant protection, and seed Se concentration.
8
Ahmed, Fazle Rabbi Sadeque. "Response of the rhizobial-mycorrhizal-lentil symbiosis to arsenic." Thesis, University of Aberdeen, 2005. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU205721.
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The thesis focused on the response of the rhizobial-mycorrhizal-lentil symbiosis to arsenic as an environmental contaminant. This was instigated by the concern over the arsenic contaminated irrigation water problem in South East Asia, and in particularly, its impact on Bangladesh agriculture. This led to a holistic approach to the study, by investigating the effect of inoculation with Rhizobium leguminosarum and the AM fungus Glomus mosseae, as well as application of superphosphate and rock phosphate, on lentil growth, nitrogenase activity and nutrient uptake (especially N and P) in the presence of arsenic contaminated irrigation water. Positive effects of mycorrhizal inoculation on lentil (Lens culinaris L.) growth, nitrogen fixation and P nutrition were observed, along with reduced uptake of As in roots, shoots and pods. Decreased plant growth, nitrogen fixation, nutrient uptake, mycorrhizal infection and increased uptake of arsenic in root, shoot and pods were observed due to application of arsenate contaminated irrigation water. The use of a lux based bacterial biosensor test demonstrated that mycorrhizal inoculation reduced arsenic bioavailability in soil and that most of the toxicity was associated with the colloidal and fine particulate soil fraction. When rock phosphate was applied, an increase of P uptake only was observed. In contrast, superphosphate increased both P and As uptake and decreased mycorrhizal infection and activity. In summary, this thesis has shown that mycorrhizal inoculation appears to offer great potential as an effective tool contributing to crop management for technique in minimisation of the total intake of As by human and livestock. The observed decreased in uptake of As into pods of mycorrhizal lentil has particularly important potential implications for human health.
9
Hamdi, Ahmed Hamdi Ismail Hamdi Ahmed. "Variation in lentil (Lens culinaris Medik) in response to irrigation." Thesis, Durham University, 1987. http://etheses.dur.ac.uk/6759/.
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This study aimed to investigate the response of lentil genotypes to different water regimes, providing guide lines, through partitioning the variation, for a selection program for adaptation to irrigated conditions. The research was divided into two main areas; 1) The overall variation in the crop was partitioned into genotypic, environmental and genotype-environmental components in an analysis of adaptation over seasons, irrigation regimes and locations; 2) The genotypic variation was partitioned into its various genetic components in an inheritance study using the dial lei mating system. Pronounced progress should be expected from selection for number of pods/plant, 100 seed weight and straw yield/plant traits, which showed high estimates of h(^2)(_b.s), C.G.V. and G.S. The two former traits correlated strongly and positively with seed yield, which allowed their use in indirect selection for seed yield. The 35 genotypes used in this study showed wide genetic diversity, allowing selection of high yielding genotypes under irrigation. Environmental variation in water supply, temperature and soil type was found to exert a profound effect on variation in characters measured. This suggests the possibility of raising yield levels through improved management practices. In this study, irrigation repeated twice increased seed yield by 19% over no irrigation, at the same location, and increased the yield by 300% in comparison with a dry location. Seed protein quality was influenced by environments and genotypes. Electrophoretic studies showed that the number and position of the bands could be used to identify genotypes. Four genotypes showed response to irrigation and could be recommended as promising entries. An anatomical study showed that large air spaces formed in the roots of a responsive genotype:, which could be used as a selection criterion for positive response to irrigation. Seed yield/plant exhibited 31.8% heterosis and showed a predominant role of non-additive genetic variance. Due to the significance of the non-additive effect, the superior F(_1)'s may be expected to throw out desirable transgressive segregants, provided that the complementary genes and epistatic effects included in the non-additive component are coupled in the same direction to maximize seed yield. Five F(_2) crosses showed superiority in seed yield and SCA effects. These crosses should be carried forward in lentil breeding programs.
10
Crook, D. G. "The introduction of autumn-sown lentil (Len culinaris) into arable cropping." Thesis, University of Reading, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.301928.
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11
Andrahennadi, Chandra Pemajayantha. "RAPD markers for ascochyta resistance, phylogenetic studies and cultivar identification in lentil." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq24066.pdf.
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12
Kelly, Rosalind. "High resolution crystallographic studies of Les culinaris agglutinin in the native and ligated states." Thesis, Liverpool John Moores University, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.388587.
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13
Kose, Fatma Selin. "Physiological And Biochemical Screening Of Different Turkish Lentil Cultivars Under Salt Stress Conditions." Master's thesis, METU, 2012. http://etd.lib.metu.edu.tr/upload/12615009/index.pdf.
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Salinity is the 2nd major limiting abiotic factor on plant growth. As a result of this, soil salinity greatly reduce the yield of the crop production by dual action on plants which are ionic toxicity and water deficit. Therefore, improvement of stress tolerance is greatly concerned. This study was performed to screen and select a salt-resistant and a salt-sensitive cultivar among 6 Turkish lentil cultivars (Lens culinaris M.) which are Çagil, Ç
iftç
i, Kafkas, Malazgirt, Seyran and Ö
zbek according to the physiological and biochemical properties. 12 days old lentil seedlings which were exposed to salt stress (100 mM NaCl and 150 mM NaCl) for 5 days as well as control groups analyzed physiologically by root-shoot fresh weights, and lengths
and biochemically by ion leakage, MDA, H
14
Adhikari, Eliina. "Genetic and Environmental Variation on Mineral Nutrient Concentration in Pea and Lentil Seed." Thesis, North Dakota State University, 2015. https://hdl.handle.net/10365/27255.
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Current food supply is expected to be insufficient to support the growing population both in quantity and nutritional quality; therefore, the need to breed for higher yield and greater nutritional quality is urgent. Twenty-five dry pea genotypes, 25 Turkish red lentil and 23 green lentil genotypes were tested across different locations of North Dakota to quantify the nutrient concentration and to characterize the genotype and the environmental factors affecting nutrient concentration. Significant genotypic, environmental and genotype-by-environment interaction was present for Ca, Cu, Fe, K, Mg, Mn, P and Zn analyzed in dry pea and lentil. A range of correlations among and/or between mineral elements and seed yield parameters was observed. This suggests that breeding for quantitative trait like mineral elements is possible through conventional breeding however; multi-location testing is very crucial for analyzing the genetic and environmental effect on mineral concentration in the seeds.
15
Kamci, Hamdi. "Optimisation Of Agrobacterium Mediated Gene Transfer And Micrografting Systems In Lentil (lens Culinaris Medik)." Master's thesis, METU, 2004. http://etd.lib.metu.edu.tr/upload/2/12605460/index.pdf.
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In this work Agrobacterium (KYRT1::pTJK136) mediated gene transfer to lentil (Lens culinaris Medik.) embriyo apex and regeneration through micro-grafting in lentil was studied.
In micro-grafting two different types root stock stem height and root stock preparations were optimized. According to the results half stem length was found to be more successful then the full. Also lentil root stock was more successful then the chickpea root stock. The types of root stock preparations studied were designated as Z and M. The Z type root stock was superior then the M type, when the micro-grafting, hardening and green-house stages were concerned.
In study of Agrobacterium mediated gene transfer to lentil embryo apex the effect of the following parameters on the transformation efficiency were addressedtype and intensity of injury, type of pre-incubation media for injured explants, effect of evacuation, effect of L-cysteine during co-cultivation and Agrobacterium incubation duration. According to the results crushing type of injury was superior over the poking and sonication type of injuries. Following the injury Hogland`s solution was used as pre-incubation media prior to infection. The effect of evacuation parameter was found to be insignificant whereas the effect of L-cysteine during co-cultivation was negative on the transformation efficiency. According to the Agrobacterium incubation duration studies, 240 minutes, followed by 120 minutes of Agrobacterium incubation were the most efficient in terms of transformation efficiency. However, since there was no significant difference among the two 120 minutes chosen to be the optimum bacterial incubation duration.
16
Stone, Anne. "The adaptation of chickpea (Cicer arietinum) & lentil (Lens culinaris) to the United Kingdom." Thesis, University of Reading, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.315526.
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17
Johnny, Liset. "Effects of interactions between arbuscular mycorrhizal fungi and Rhizobium leguminosarum on pea and lentil." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp02/NQ37892.pdf.
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Gupta, Debjyoti Sen. "Genetic and Phenotypic Assessment of Iron and Folate Concentration in Lentil (Lens Culinaris Medik.)." Diss., North Dakota State University, 2015. http://hdl.handle.net/10365/25321.
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Micronutrients and vitamins are chemical elements required in trace quantities for normal human growth and development. Micronutrients and vitamin deficiency is prevalent throughout the world. The first objective of this research was to determine folate concentration in 10 lentil genotypes and evaluate the effect of environment on folate concentration. Folate concentration ranged from 216 to 290 ?g/100 g with a mean of 255 ?g/100 g and the concentration differed across years and locations. A significant genotype ? environment interaction effect was observed for lentil folate concentration. The second objective was to measure the iron, zinc, copper, calcium and magnesium concentration in 26 cultivated and wild lentils. Significant variation in Fe, Zn, Cu, Ca, and Mg concentration among Lens species and no single genotype had high concentrations of all micronutrients.The third objective was to determine genetic diversity among 29 cultivated and wild lentils using 39 simple sequence repeat markers. Thirteen of 39 SSR markers were polymorphic among the 29 lentil genotypes. Cluster analysis grouped the genotypes into 4 clusters broadly based on the genotyping data and this grouping had correspondence with the pedigree relationships of the genotypes. The fourth objective was to develop expressed sequence tags-simple sequence repeats (EST-SSRs) markers in lentil. Lentil EST sequences (9513) from the NCBI database were assembled into 4053 unigenes. Unigenes were screened for simple sequence repeats and 348 primer pairs were designed. Fifty-seven primer pairs were polymorphic among the 22 lentil genotypes providing additional gene-specific primers for use in lentil breeding. The fifth objective was to develop gene specific molecular markers for iron metabolism related genes in lentil and to study their gene expression in the presence of excess iron. Gene specific markers were developed for Ferritin-1, BHLH-1, and IRT-1 to allow detailed study of the iron metabolic pathway in lentil. Differential gene expression of Ferritin-1 and IRT-1 under excess iron was observed at 2 hours but not at 8 hours and 24 hours. Results of these studies contribute to a broad understanding of the genetic variation, environmental influence on and expression of genes related to micronutrient and vitamin concentration and metabolism in lentil.ND Agricultural Experiment Station, NDSU
Northern Pulse Growers Association
USA Indian Council of Agricultural Research (ICAR)
Ministry of Agriculture, Government of India
International Center for Agricultural Research in the Dry Areas (ICARDA), Morocco
19
Muñoz, Alejandro Matus. "Carbon isotope discrimination and indirect selection for grain yield in lentil, spring wheat and canola." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1996. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq25213.pdf.
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Viguier, Loïc Arthur. "Analysis of the agronomic and economic performances of lentil-spring wheat intercrops in organic farming." Phd thesis, Toulouse, INPT, 2018. http://oatao.univ-toulouse.fr/23674/1/Viguier_Loic1.pdf.
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Lentil (Lens culinaris Med.) is an important component of the human diet in the world, but in the meantime, Europe produces only 26% of the lentils it consumes. This is partly due to strong agronomic weaknesses that reduce yield such as lodging, bruchid beetles and weeds, especially in organic farming. Intercropping, the simultaneous growing of two or more species in the same field is tested here as an option to reduce these drawbacks and develop organic lentil production. The aims of this thesis were to (1) assess the potential of lentil-spring wheat intercrops to produce organic lentil, (2) understand the mechanisms that explain their performances, and (3) evaluate the profitability of such intercrops. A two-year field experiment was carried out in southwestern France in 2015 and 2016 under organic farming rules. Four lentil and two wheat cultivars were grown as sole crops and intercrops in multiple additive and substitutive designs. Our results showed that the total intercrop attainable grain yield was higher than the mean of sole crops. Yet, lentil yield in intercrop was lower than in sole crop as the result of a strong competition for resources from wheat in early lentil growth stages reducing the number of branches per plant of lentil. This led to lower gross margins of intercrops. However, lentil lodging was strongly reduced in intercrops thus its mechanical harvest efficiency increased. This led to similar mechanically harvested yields of lentil in intercrop and sole crop. Consequently, after mechanical harvest and grain cleaning, the marketable gross margin of intercrops was higher than that of sole crops. Our results suggest that (1) intercrop had no effect on bruchids, (2) the most effective intercrop is when lentil is at sole crop density and wheat at 15-20%, (3) intercrop performance is due to complementary use of N pools through legume N2 fixation and (4) the intensity of interspecific interactions depends on year, wheat density and genotypes. Our work indicates that lentil-spring wheat intercrop can develop organic lentil production but a better understanding of Genotype x Environment x Cropping system interactions may be useful to design optimized managements.
21
Moffet, Matthew Durwin. "Identifying regions of conserved synteny between pea (Pisum spp.), lentil (Lens spp.), and bean (Phaseolus spp.)." Thesis, Montana State University, 2006. http://etd.lib.montana.edu/etd/2006/moffet/MoffetM1206.pdf.
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The identification of conserved synteny in legumes can facilitate many different types of gene discovery. Techniques like marker assisted selection and the candidate gene approach can benefit greatly by identifying conserved synteny and genes located within those regions. Both Pisum and Phaseolus have detailed linkage maps, but a limited number of markers have been located in both species. In the present study I mapped 21 genes in Phaseolus vulgaris, 16 of which had already been located on the Lens and Pisum sativum linkage maps, the markers were used to look for conserved synteny between Pisum, Lens and Phaseolus. In particular, I was able to target marker/gene-rich regions of pea linkage groups V and VII, as well as pea linkage group III, with Pisum STS markers and universally designed gene-specific markers already located on the pea linkage map. About 50% of the tested genes amplified an appropriate sized fragment in Phaseolus, but less than 40 % of the gene-specific markers showed polymorphism by cleaved amplified polymorphic sequence (CAPS) analysis in bean. The data reveals little evidence for extensive gene order conservation, and even some closely linked (<5cM) loci in Pisum are not linked in Phaseolus vulgaris. The only example of conserved synteny was approximately 15cM on pea and lentil linkage group V and bean linkage group 1. Paal, Enolase, and TufM were first identified in the syntenic area and allowed identification of fin/det, one of several TFL genes already mapped in bean, as another orthologous loci between pea and bean. Finding conservation of synteny with Paal, identified Paal3 and TFL1 genes as linked loci in Arabidopsis thaliana on linkage group 5. The pea locus Paal1,2 is then speculated to be a tandem duplication of a Paal3 homolog in a ancient common ancestor and probably occurring after the speciation of Pisum.
22
Celikkol, Akcay Ufuk. "Regeneration Of Lentil (lens Culinaris Medik) And Genetic Transformation By Using Agrobacterium Tumefaciens-mediated Gene Transfer." Phd thesis, METU, 2008. http://etd.lib.metu.edu.tr/upload/3/12609425/index.pdf.
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In this study, the effects of different plant growth regulators on regeneration responses of various lentil explants through direct and indirect organogenesis and through somatic embryogenesis from calli and cell suspension cultures were investigated. Shoot regeneration was obtained in low frequencies from longitudinal embryonic axis explants and nodal buds of epicotyls, however whole plant regeneration was unsuccessful. Conditions provided for indirect organogenesis resulted only in swelling of hypocotyls and root directed ends of internodes and weak callus formation on leaves which were followed by tissue browning and necrosis. In somatic embryogenesis studies, the explants longitudinal embryonic axis and cotyledonary petioles produced soft and friable calli on MS media with Gamborg&rsquos vitamins supplemented with 0.75mg/L 2,4-D+0.5mg/L BA. The highest average number of embryos per explant, 12.36 was observed on media containing 0.75mg/L BA +0.5mg/L 2,4-D for cotyledonary petiole explants, whereas 3mg/L BA+1mg/L NAA was the only hormone combination that allowed embryo development to some extent, in both explants. Somatic callus failed to regenerate despite globular embryo formation and embryo development to some extent. Combination of sonication treatment with Agrobacterium transformation of three lentil explants
cotyledonary nodes, half cotyledons and cotyledonary nodes with intact shoots, had no effect on the improvement of transient gus gene expression on explants. Sonication treatment was also unable to form localized wounds on the petiole axils. The best gus gene expression on the axil region was obtained when cotyledonary nodes and KYRT1 strain were used in combination with vacuum infiltration and scalpel wounding of the axils. Gradual selection and repeated removal of regenerated shoots between selection cycles increased the number of gus expressing shoots significantly. The regenerated shoots were grafted on root stocks and whole plant regeneration was achieved in greenhouse conditions. By the use of the optimized Agrobacterium-mediated transformation protocol, 4 independent lines were obtained with 2.3% transformation efficiency. Southern blot analysis confirmed the integration of the gus gene into the genome of lentil plants. T0 plants were fertile and all plants showed Mendelian segregation of the gus gene in 3:1 ratio to their progenies except one line which carries three copies of the gene. Reverse transcription PCR has confirmed the expression of the genes in T0 and T1 generations. T0 plants and the following three generations strongly expressed gus gene uniformly in their tissues and the PCR amplifications of both gus and npt-II genes was successful through generations.
23
Gokcay, Derya. "Physiological And Biochemical Screening Of Different Turkish Lentil (lens Culinaris M.) Cultivars Under Drought Stress Condition." Master's thesis, METU, 2012. http://etd.lib.metu.edu.tr/upload/12615008/index.pdf.
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Legumes being the most important crops worldwide are limited in terms of adaptability and productivity mainly by the abiotic stresses. In this study, the aim was to understand tolerance mechanisms of lentil cultivars under drought stress by physiological and biochemical analyses. This study was carried out with six Turkish Lentil cultivars (Seyran, Kafkas, Malazgirt, Çagil, Ç
iftç
i, Ö
zbek) subjected to drought stresses (10% and 15% PEG) and their physiological and biochemical properties were examined to select drought-tolerant and drought-sensitive cultivars. Drought stress was applied for 5 days to 7 days-grown lentil plants. 12-days old, stressed and control plant shoots and roots were analyzed in terms of physiological and biochemical parameters (length, fresh weight, ion leakage, proline, MDA and H
24
Bayrac, Abdullah Tahir. "Optimization Of A Regeneration And Transformation System For Lentil (lens Culinaris M., Cv. Sultan-i) Cotyledonary Petioles And Epicotyls." Master's thesis, METU, 2003. http://etd.lib.metu.edu.tr/upload/3/12605453/index.pdf.
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In this study, optimization of a transformation and regeneration system via indirect organogenesis in cotyledonary petiole tissue of lentil (Lens culinaris Medik.) was investigated. Eight different medium types differing in their plant growth regulator compositions were employed to examine the callus induction potency of cotyledonary petiole. Except two, all other tested medium yielded more than 80% callus induction. Nine different medium types were studied to test the potencies of callus structures for shoot induction. Only the callus induced in medium H (1 mg/L Zeatin riboside + 1 mg/L Naphthalane acetic acid) yielded shoots at 8 to 40 % frequency. The most responsive medium was MS basal medium with no growth regulators. Also five and three different medium types were employed to examine callus induction potency of epicotyl tissues respectively. Each medium type yielded 90% callus induction. Only the callus induced in medium H yielded shoots At 6 to 26% frequency.
Preliminary studies were carried out for somatic embryogenesis in cotyledonary petiole. Effects of salicylic acid on somatic embryogenesis were also investigated. Salicylic acid at 200µM was found to enhance the percentage of somatic embryos by 25 % and reduce the necrosis 24 %. However none of the globular and heart shape embryos were able to regenerate. Transient GUS expression efficiencies of roots, shoot tips, and cotyledonary petioles were tested after Agrobacterium-mediated transformation. Transformation frequencies were 26, 74, and 38 % for cotyledonary petiole, shoot tips, and roots respectively.
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Cicerali, Isin Nur. "Effect Of Salt Stress On Antioxidant Defense Systems Of Sensitive And Resistant Cultivars Of Lentil (lens Culinaris M.)." Master's thesis, METU, 2004. http://etd.lib.metu.edu.tr/upload/3/12605248/index.pdf.
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ABSTRACT
EFFECT OF SALT STRESS ON ANTIOXIDANT DEFENSE SYSTEMS OF
SENSITIVE AND RESISTANT CULTIVARS OF LENTIL
Cicerali, Iin Nur
M.Sc., Department of Biotechnology
Supervisor: Prof. Dr. Meral Yücel Co-supervisor: Asst. Prof. Dr. Fü
sun (nci) Eyidoan June 2004, 90 pages In this study, two lentil cultivars (Lens culinaris, Medik.) (ILL5582-salt tolerant and ILL590) were characterized and compared due to their NaCl susceptibility and antioxidant mechanism was examined under laboratory conditions. Physiological parameters such as wet-dry weight, root-shoot lengths, cell membrane stability, lipid peroxidation in terms of malondialdehyde (MDA), H2O2, proline contents were determined. The activities of antioxidant enzymes such as superoxide dismutase (SOD: EC 1.15.1.1), catalase (CAT: EC 1.11.1.6), ascorbate peroxidase (APX: EC 1.11.1.11) and glutathione reductase (GR: EC 1.6.4.2) were examined and analyzed in 14 days old plant seedlings after 9 days of normal growth and 5 days of 100mM and 200mM NaCl stress conditions. Shoot-root length and wet-dry weight percent decrease were more in ILL590. Especially shoot tissues were affected more from the stress conditions when compared to root tissues. ii According to malondialdehyde (MDA) content and membrane stability results, lipid peoxidation was higher in ILL590 and significant increases were observed in shoot tissues. Proline concentration showed a remarkable increase in salt concentration dependent manner. Higher concentrations of proline in ILL5582 might be the reason of higher salt tolerance when compared to ILL590. Among the antioxidant enzymes SOD was the one which showed highest activity increase. At organ level roots showed highest activity when compared to leaves. In the organelle higher activity percent contribution was achieved by cytosolic Cu/ZnSOD isozyme. Higher percent increase of this isozyme was observed in ILL5582. This might be one of the tolerance mechanisms that get activated against NaCl stress. APX activity showed similar alterations in both cultivars. In leaf tissues significant increase was observed but in root tissues ascorbate peroxidase activity did not change significantly. Glutathione Reductase activity increase was significant in both cultivars leaf tissues but although ILL5582 showed a stress concentration dependent increase, ILL590 did not. The activity of CAT enzyme in leaf and root tissues of both cultivars did not significantly change under increasing salt stress conditions. The results suggested that the leaves were more susceptible to salt stress. Also when two cultivars were compared ILL5582 was found to be more tolerant against salt stress than ILL590 under laboratory conditions and SOD enzyme seemed to be the most active component of the salt tolerant mechanism.
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Ercan, Oya. "Effect Of Drought And Salt Stresses On Antioxidant Defense System And Physiology Of Lentil (lens Culinaris M.) Seedlings." Master's thesis, METU, 2008. http://etd.lib.metu.edu.tr/upload/3/12609250/index.pdf.
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In this study, 14 days old lentil seedlings (Lens culinaris Medik cv. Sultan), which were subjected to 7 days of drought (20% PEG 6000), and salt (150 mM NaCl ) stress , were examined in a comparative manner for the effects of drought and salt stress treatments. In shoot and root tissues physiological parameters such as wet-dry weight, relative water content, root-shoot lengths, membrane electrolyte leakage, and lipid peroxidation in terms of malondialdehyde (MDA) were determined. H2O2 content, proline accumulation and chlorophyll fluorescence analysis were also performed. Changes in the activity of antioxidant enzymes such as superoxide dismutase (SOD: EC 1.15.1.1), catalase (CAT: EC 1.11.1.6) ascorbate peroxidase (APX: EC 1.11.1.11) and glutathione reductase (GR: EC 1.6.4.2) were observed upon stress treatments. In salt treated lentil seedlings, significant decreases in wet-dry weight, RWC, shoot-root length and chlorophyll fluorescence measurements indicated a sensitivity, when compared to drought treated plants. Higher MDA concentration and higher electrolyte leakage amounts are supported these results. APX, GR and proline seem to play important roles in antioxidant defense against salt stress for both tissues by removing reactive oxygen species and protecting macromolecules and membranes. GR and proline are also maintains the main protective mechanism against drought stress effects. SOD is active in drought stressed roots and salt stressed shoots, where the H2O2 contents are also observed to be increased.
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Mooleki, Siyambango Patrick. "Synchronization of nitrogen availability and plant nitrogen demand, nitrogen and non-nitrogen effects of lentil to subsequent wheat crops." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0029/NQ63902.pdf.
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Wang, Lina [Verfasser], and Wilhelm [Akademischer Betreuer] Claupein. "Lentil production in Germany : testing different mixed cropping systems, sowing dates and weed controls / Lina Wang. Betreuer: Wilhelm Claupein." Hohenheim : Kommunikations-, Informations- und Medienzentrum der Universität Hohenheim, 2012. http://d-nb.info/1027354211/34.
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Aksoy, Emre. "Effect Of Drought And Salt Stresses On The Gene Expression Levels Of Antioxidant Enzymes In Lentil (lens Culinaris M.) Seedlings." Master's thesis, METU, 2008. http://etd.lib.metu.edu.tr/upload/3/12609960/index.pdf.
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This study was carried out for understanding of antioxidant mechanisms of lentil under abiotic stress conditions. For this aim, 14 days old lentil seedlings (Lens culinaris Medik cv. Sultan-1) were subjected to drought (20% PEG 6000), and salt (150 mM NaCl ) stress for 6, 12 and 24 hours, for 3, 5 and 7 days. PCR conditions for Mn SOD, Cu/Zn SOD, chloroplastic/mitochondrial GR, CAT and chloroplast /stromal APX antioxidant enzymes were optimized. Then, total RNA was isolated from stressed and non-stressed plant roots and shoots. The gene expression levels of Mn SOD and Cu/Zn SOD were examined by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) technique. Arabidopsis 18S rRNA was used as internal control in multiplex PCR technique. Relative expression levels of Mn SOD were lower in shoots and roots under salt stress while no significant change was obtained under drought conditions in both tissues. Relative expression levels of Mn SOD were increased on 5th day of salt and drought applications in both shoots and roots. Relative expression levels of Cu/Zn SOD increased after 5th, and on 1st and 7th days of drough treatment in shoots and roots, respectively. On the other hand, expression levels of Cu/Zn SOD increased on 3rd and 5th days of salt treatment in shoot tissues. Although it is nearly impossible to understand the whole antioxidant mechanism of plants under environmental stresses, this study was the first step to learn about molecular background of antioxidant defence mechanisms in lentil.
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Kamci, Hamdi. "Genetic Transformation Of Lentil ( Lens Culinaris M. Cv.sultan.1) With A Transcription Factor Regulator (mbf1c) And Analysis Of Transgenic Plants." Phd thesis, METU, 2011. http://etd.lib.metu.edu.tr/upload/12613845/index.pdf.
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iv
ABSTRACT
GENETIC TRANSFORMATION OF LENTIL ( Lens culinaris M. cv.Sultan.1)
WITH A TRANSCRIPTION FACTOR REGULATOR (MBF1c)
AND
ANALYSIS OF TRANSGENIC PLANTS
KAMÇI, Hamdi Ph.D., Biotechnology, Institute of Natural ad Applied Sciences Supervisor Prof. Dr. Meral YÜ
CEL Co-Supervisor: Dr. Ufuk Ç
elikkol AKÇ
AY September 2011, 252 pages In this study, Agrobacterium mediated genetic transformation of lentil Sultan 1 cultivar with MBF1c and evaluation of transgenic plants was aimed. The study was initially based on optimized protocol with Agrobacterium tumefaciens KYRT1 strain and pTJK136 binary plasmid. Based on this protocol and transient marker gene expression in embryo apex, 15% stable transformation efficiency was aimed. However limited knowledge about pTJK136 and problem with curing KYRT1 leaded us to use Agrobacterium tumefaciens C58C1 strain and also to engineer an alternative binary plasmid
pPZP101. Hence, scope of this study became construction of a plant binary transformation vector and lentil transformation optimization with C58C1 strain.First plant transformation vector designed in this study was pPZP101ManA-MBF1c. Transformations with C58C1::pPZP101ManA-MBF1c were carried out with a reformulated co-cultivation media. Cotyledonary nodes were isolated from three days old lentil seedlings germinated with phytormone (BAP/TDZ) induction. Isolated nodes were either injured and pre-incubated in co-cultivation media or pre- incubated and then injured prior to transformation. Regeneration and necrosis behaviors of the transformed explants leaded us to the conclusion that explant preparation is the critical step of transformation. And data suggest that explants isolated from 2mg/l BAP, pre-incubated two days in co-cultivation media, injured and transformed performed significantly better scores for necrosis shoot regeneration and callus formation parameters. Transformed explants that survived in subsequent sub-cultures in mannose selection raised shoots. These shoots were grafted and regenerated into plantlets. The putative transgenic plantlets were screened for transgene with PCR. Initial amplification signals fainted and lost as grafts grew. In order to make a diagnosis of this fainting behavior the second plant transformation vector pPZP101ManA- GUSint-MBF1c was constructed and transient GUS expression analysis were made.
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Gayer, Christoph [Verfasser], and Martin [Akademischer Betreuer] Dieterich. "Suitability of conventional flowering fields and organic lentil mixed-crops to promote biodiversity on arable land / Christoph Gayer ; Betreuer: Martin Dieterich." Hohenheim : Kommunikations-, Informations- und Medienzentrum der Universität Hohenheim, 2020. http://d-nb.info/1223981924/34.
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PAGLIARO, ANTONELLA. "Pasta with cholesterol-lowering effect on human health." Doctoral thesis, Università degli Studi di Foggia, 2019. http://hdl.handle.net/11369/382621.
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La pasta è un alimento consumato giornalmente che provvede a fornire energia, interviene nella percezione del senso di sazietà ed esercita effetti positivi sulla salute umana. Inoltre la pasta rappresenta un buon veicolo per l’assunzione di biomolecole essendo uno degli alimenti principali della dieta mediterranea. Scopo principale di questo lavoro, è stato ottenere nuove paste funzionali ad azione ipocolesterolemizzante. Per raggiungere l’obiettivo connesso con l’attività di ricerca è stata effettuata la valutazione delle proprietà reologiche, sensoriali e chimiche di paste prodotte con farina di grano duro commerciale addizionate con lenticchia siciliana (Lens culinaris Medik) ricca in amminoacidi essenziali, cladodi di popolazioni siciliane di fichi d’india (Opuntia ficus-indica (L.) Mill ricchi in fibre, specie selvatiche siciliane di portulaca (Portulaca oleracea L.) ricche in acidi grassi e farina di avena ricca in β-glucani. Grazie all’uso dell’additivo alimentare carbossimetilcellulosa (CMC), gli spaghetti arricchiti con il 40% di lenticchia presentavano una buona valutazione sensoriale e un miglioramento della qualità in cottura. Tutte le paste arricchite in lenticchia sono risultate una buona fonte di amminoacidi essenziali, l’aggiunta della lenticchia di Ragusa ha aumentato il contenuto di Lys, Val, Ile, Gly e Asp, mentre l’aggiunta della lenticchia commerciale ha aumentato il contenuto in Leu, Thr, Ser e Lys. Con riferimento alle paste alimentari arricchite con il 5% e 10% di cladodi di ficodindia e CMC è stato possibile ottenere sufficienti caratteristiche sensoriali, ma buone caratteristiche nutraceutiche, essendo risultate fonte di fenoli e fibre. Riguardo l’aggiunta di farine di portulaca, l’analisi sensoriale delle paste prodotte ha mostrato che il giudizio globale diminuiva considerevolmente negli spaghetti con il 15% di portulaca, mentre quelli con il 10% di portulaca sono stati apprezzate. Le analisi chimiche hanno evidenziato che la pasta arricchita in portulaca ha tutto il potenziale per essere un cibo funzionale, grazie al suo contenuto in acidi grassi e la sua capacità antiossidante. Tutte le paste arricchite con quattro diverse concentrazioni di avena hanno avuto punteggi positivi nell’analisi sensoriale. I β-Glucani sono stati estratti e caratterizzati nell’avena aggiunta e negli spaghetti prodotti. Il contenuto in β-Glucani aumentava aumentando la concentrazione di avena aggiunta e soprattutto non sono state riscontrate perdite dopo il processo di cottura. In generale, i livelli di accettabilità raggiunti da parte dei consumatori, abbinate alle evidenze nutrizionali e chimiche dei prodotti ottenuti rappresentano un punto di partenza per ulteriori ricerche in campo clinico, al fine di valutare l'effetto diretto sul livello del colesterolo in pazienti affetti da ipercolesterolemia.Pasta is a daily food that provides energy, allows the perception of the satiety sense of and has positive effects on health on human health. However, pasta is a good vehicle for the intake of biomolecules, being one of the main foods in the Mediterranean diet. The aim of this study was the production of new type’s pasta that have the potentiality to decrease cholesterol. To reach the aim of the project were evaluated sensorial, rheological and chemical characteristics of pasta samples produced using durum wheat, added with Sicilian populations of lentil (Lens culinaris Medik.) rich in essential amino acids, cladodes of prickly pear (Opuntia ficus-indica (L.) Mill) Sicilian populations rich in fiber, typical wild species of purslane (Portulaca oleracea L.) rich in fatty acids and with oat flour rich in β-glucans. Thanks to the use of food additive carboxymethylcellulose (CMC), spaghetti enriched with 40% of lentil had good results, both in sensorial analysis and in the cooking quality evaluation. All Pasta samples enriched in lentils resulted a good source of essential amino acids, the addition of Ragusa lentil improved the contents in Lys, Val, Ile, Gly and Asp, while the amounts in Leu, Thr, Ser and Lys resulted improved in spaghetti enriched by commercial lentil. As regard pasta enriched with 5% and 10% of prickly pear cladodes and CMC, it was possible to obtain sufficient sensorial characteristics, but good nutraceutical properties, being a good source of phenols and fiber. Referring the addition of purslane flour, the sensorial analysis showed that the scores of the global judgment decreased considerably in pastas with 15% of purslane, while pasta samples with 10% of purslane were appreciated. Chemical analysis highlighted that pasta enriched with purslane has the potentiality to be a functional food, thanks to its polyunsatured fatty acids concentration and antioxidant activity. All pasta samples enriched in oat at four different concentrations had positive scores in sensorial analysis. β-glucans were extracted and characterized in oat and in produced pasta samples. The content of β-glucans increased increasing the concentration of added oat and especially were not recorded cooking loss. The reached levels of consumer acceptance, together with the chemical and nutraceutical evidence of the obtained products, could be the starting point in the clinical field, in order to evaluate the direct effect on cholesterol level in patients with hypercholesterolemia.
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Ruland, Michael [Verfasser], Heiko C. [Akademischer Betreuer] Becker, Gunter [Gutachter] Backes, and Klaus [Gutachter] Dittert. "Site-specific adaptation by natural selection. A case study with lentil. / Michael Ruland ; Gutachter: Gunter Backes, Klaus Dittert ; Betreuer: Heiko C. Becker." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2018. http://d-nb.info/1156008298/34.
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Sá, Jocelito Saccol de. "Influência do manejo do nível freático e doses de nitrogênio em culturas sob hipoxia no solo." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/11/11143/tde-10032006-164551/.
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Em diversos países, extensas áreas agrícolas são afetadas por inundações temporárias que ocasionam significativos prejuízos à produção de alimentos. Os objetivos deste trabalho foram: a) avaliar os efeitos da exposição de plantas de lentilha (Lens culinaris Medik) ao estresse por excesso hídrico em relação ao manejo do nível freático; b) avaliar o comportamento de cultivares de soja (Glycine max L. Merrill) a hipoxia e à aplicação de nitrogênio na atenuação dos danos ocasionados pelo excesso hídrico e c) avaliar as respostas de plantas de trigo (Triticum aestivum, L.) à saturação hídrica, total ou parcial, da camada de solo correspondente à zona radicular da cultura. Em plantas de lentilha, o excesso de água no solo reduziu a expansão foliar, o acúmulo de biomassa, a produção de grãos e reduziu a concentração de N, K, Ca, Mn e Mg nas folhas e intensificou o processo de senescência foliar. As taxas de ascensão e de rebaixamento do nível freático de 15 e 30 cm dia-1 influenciaram positivamente o desenvolvimento e a produção. Os danos mais drásticos foram ocasionados pela elevação e pelo rebaixamento do nível freático em 5 cm dia-1. O efeito negativo da inundação do solo na produção de grãos foi reduzido em 21% pelo aumento da taxa de ascensão e de rebaixamento do nível freático de 5 para 30 cm dia-1. A adição de 50 kg N ha-1 não minimizou os danos ocasionados pela hipoxia. Em soja, a área foliar, o acúmulo de matéria seca, a produção e a qualidade dos grãos dos cultivares decresceram com o prolongamento da inundação do solo. Os teores foliares de N, K, Mg e Mn decresceram linearmente e a concentração de Fe aumentou com a inundação do solo. O cultivar FT-Abyara apresentou maior crescimento vegetativo e o cultivar CD205 maior produtividade e maior teor protéico. Os maiores danos à produção foram ocasionados pela inundação do solo por período superior a 48 h. A aplicação de 50 e 100 kg N ha-1 aumentou o crescimento da parte aérea das plantas, a produção e a concentração de proteína dos grãos. O cultivar FT-Abyara apresentou melhores resultados à adição de N. A aplicação de N reduziu em 15% os danos causados pelo excesso de água no solo à produção de grãos. Em plantas de trigo, a saturação hídrica do solo intensificou a senescência das folhas, reduziu a produção de matéria seca, a taxa de crescimento da cultura e prejudicou a produção de grãos. Os efeitos ocasionados pela ocupação total dos poros do solo pela água foram mais severos para o desenvolvimento e a produção de grãos do que a saturação parcial dos poros pela água. O nível freático a 15 cm de profundidade minimizou em 30% os danos ocasionados pelo excesso de água no solo à produção de grãos. A aplicação de 50 kg N ha-1 reduziu em 16% os danos ocasionados pela saturação hídrica do solo à produção de grãos.In many countries, extensive agricultural areas are affected by short-term waterlogging that cause significant damages to the crop yields. The objectives of this work were: a) to evaluate the stress effect of the soil water excess on lentil plants (Lens culinaris Medik) related to water table management; b) to evaluate the behavior of soybean cultivars (Glycine max L. Merrill) to hypoxia conditions and to the nitrogen supplying for reducing flood damages c) to evaluate wheat (Triticum aestivum, L.) responses to complete or partial soil water saturation in the root zone. On lentil plants the excess of water reduced the growth of the leaves, the dry matter and grain production and the concentration of N, K, Ca, Mn and Mg in leaves. Also the senescence process was increased. The elevation and lowering by 15 and 30 cm day-1 affected positively the crop development and yield production. The most severe damage was induced by rate elevation of 5 cm day-1 and drawdown of water table. The increasing of water table ascension and lowering rate from 5 to 30 cm day-1 alleviated the damage effect on grain production in 21%. Hypoxia injuries were not minimized by a supplemental addition of 50 kg ha-1 of nitrogen. The leaves area, shoot dry matter accumulation, the grain production and the grain protein concentration of soybean plants were reduced proportionally to the flooding duration. The N, K, Mg and Mn concentration in leaves decreased linearly while Fe concentration increased for the waterlogging duration. The soybean cultivar FT-Abyara showed the higher vegetative growth, while CD205 produced a greater grain yield and grain protein concentration. A flooding period of 48 h caused severe damage to grain yield. The leaf area, shoot growth, grain yield and grain protein increased with supplemental N application of 50 and 100 kg N ha-1. The soybean cultivar FT-Abyara showed the best results due to the nitrogen supply. The N addition reduced the grain production damages by 15%. In wheat plants, the water excess increased leaves senescence, reduced dry matter and the growth rate and produced a significant decreasing on grain weight. The effects caused by total water saturation of soil pores were more severe to the plant growth and grain yield than those caused by partial water saturation, even for large periods. A water table at 15 cm depth minimized by 30% the harmful effects of water excess to the grain production. The application of 50 kg N ha-1 reduced by 16% the damages on the crop yield caused by flooding.
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Covell-Barrett, Sarah. "The influence of temperature on seed germination rate in lentil (Lens culinaris and Medic.) barley (Hordeum vulgare L.) : implications towards the breeding improvement of crop establishment." Thesis, University of Reading, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.253812.
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Liechti, Stella. "Lentille dielectrique pour l'optimisation du gain dans l'axe d'un radiotelescope millimetrique." Paris 7, 1987. http://www.theses.fr/1987PA077015.
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Construction et test d'une lentille en teflon travaillant entre 75 et 115 ghz, utilisee par le radiotelescope de 30 metre de l'iram. Le gain dans l'axe mesure sur des sources astromiques a augmente de 7 a 15%, en bon accord avec les previsions
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Zare, Fatemeh. "Supplementation of beverage, yogurt and probiotic fermented milk with lentil flour and pea flour and study of the microbial, physical and sensory properties of supplemented products after production during storage." Thesis, McGill University, 2011. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=104780.
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1-4% of pulse fractions including pea protein and fiber, chickpea and lentil flour as well as soy flour and protein concentrate were selected and characterized. As preliminary results the functional properties of pulse ingredients are varied upon their protein content and pH of the food carrier. Orange juice, apple juice, yogurt and two probiotic fermented milk were selected for supplementation. 1% and 2% pulse fractions gave comparable results in terms of turbidity, cloud and visual stability, color and sensory attributes for both orange and apple juices beverages. All supplements improved the acidification rate of yogurt and probiotic cultures, but the highest effects were obtained with probiotic supplementation with lentil and soy flour. As for the main study, skim milk (9.5 % w/v solid content) was supplemented with 1-3% (w/v) lentil flour, pea flour or skim milk powder and they were inoculated with yogurt starter cultures or probiotic (L. rhamnosus). Acid production during the fermentation, the pH, syneresis, color, rheological properties (dynamic oscillation temperature sweep test at 4-50 ˚C), and sensory properties (only for yogurt) were studied after production and 28 days of refrigerated storage.1-3% lentil and pea flour enhanced acid production during yogurt fermentation, but the microbial population (CFU) of both S. thermophilus and L. bulgaricus were in the same range in all lentil and pea flour and skim milk supplemented yogurts, after production. Pea flour supplementation enhanced survival of L. bulgaricus after storage. The pH decreased from 4.5 to 4.1 in lentil flour and from 4.5 to 3.75 in pea flour supplemented yogurts, after 28 days. Syneresis in 1-2% lentil and pea flour supplemented yogurts was higher than other samples. In lentil supplemented yogurts, "a" and "L" values did not significantly differ in all samples and remained constant after 28 days whereas, "b" value increased as a result of supplementation. Pea flour supplementation did not alter redness or greenness of yogurts, but the yellowness was significantly higher than other yogurts. Yogurt with 3% lentil and pea flour had higher storage (G΄) and loss (G˝) moduli in comparison with samples supplemented with 1-3% skim milk and the control yogurt. 1-2% lentil and pea flour supplemented yogurt showed comparable sensory properties in comparison with 1-2% skim milk supplemented and control samples.1-3% lentil and pea flour enhanced acid production during probiotic fermentation, and the CFU's of L. rhamnosus were comparable with non-supplemented control sample after production. After 28 days, the CFU`s of 2% and 3% lentil supplemented probiotic were as high as 1% skim milk supplemented sample and the CFU`s of 3% pea flour supplemented probiotic was the highest followed by 3-2% skim milk and 1-2% pea flour supplemented samples. The pH decreased from 4.50 to 3.90 for lentil flour supplemented probiotics and from 4.50 to 4.04 for pea flour supplemented probiotics, over 28 days. Syneresis in 1-3% lentil and pea flour supplemented probiotic was significantly lower than other samples. All lentil flour supplemented samples had significantly lower "L" values and higher "b" and "a" values in comparison with skim milk supplemented samples. Pea flour supplementation slightly changed the color which was not as light as skim milk supplemented samples and they showed more yellowness in final product after production and storage. Probiotic fermented milk with 1-3% lentil and pea flour showed higher G΄ and G˝ in comparison with other samples.Des légumineuses tels que des protéines et fibres de pois, farine de pois chiche, de lentille et de soja ont été sélectionnées et caractérisés. Des résultats préliminaires ont montré que des propriétés fonctionnelles ont variés en fonction de la teneur en protéines et du pH des légumineuses employées. Du jus d'orange et de pomme, du yogourt et deux laits fermentés à l'aide de probiotiques ont été supplémentés avec les différentes légumineuses à des taux de 1 à 4%. Les supplémentations à 1 et 2% ont donné des résultats comparables en termes de turbidité, de stabilité, de couleur et d'attributs sensoriels pour les jus d'orange et de pomme. L'addition de légumineuse a permis d'avoir une acidification plus rapide dans les yaourts et les cultures probiotiques, mais le effet le plus important a été obtenu avec farine de lentilles et le soja dans les cultures probiotiques. Comme précédemment, des laits écrémés (9,5% p/v) ont été supplémentés avec 1-3% (p/v) de farine de lentilles, de pois ou de poudre de lait écrémé. Ils ont été inoculés avec des cultures de yogourt, des probiotiques (L.rhamnosus). La production d'acide lors de la fermentation, le pH, la synérèse, la couleur, les propriétés rhéologiques (essai dynamique balayer oscillation de température à 40-50˚C), et les propriétés sensorielles (uniquement pour les yogourts) ont été étudiés après la production et durant 28 jours d'entreposage frigorifique. 1-3% de farine de lentilles ou de pois ont amélioré la production d'acide pendant la fermentation du yogourt, mais les UFC ont les même compte pour les laits suppléments que pour les témoins (lait écrémé). Il est a noter que L. bulgaricus avaient un meilleur taux de survie au jour 28 avec une supplémentation en farine de pois. La diminution du pH dans les yogourts est de 4,5 à 4,1 avec la farine de lentille et de 4,5 à 3,75 avec farine de pois, après 28 jours. La synérèse pour les yogourts supplémentés à 1 et 2% avec de la farine de lentille ou de pois était supérieure d'autres échantillons. Lorsque le taux de supplémentation augmente en farine de lentille ou de pois, il n'y a pas de différence significative pour les valeurs de a alors que la valeur b a augmenté en fonction de la supplémentation.Les yogourts faits de 1 a 3 % farine de lentilles et de pois 1 3% avaient un module élastique (G') et un module visqueux (G˝) plus élevés que les échantillons supplémentés en lait écrémé et que les témoins. Les Yogourts avec 1 à 2% de farine de lentilles et de pois possèdent des propriétés sensorielles comparable a celles des yogourts faits avec 1 a 2% de lait écrémé et celles des témoins. 1-3% de farine de lentilles ou de pois dans des laits avec probiotiques ont amélioré la production d'acide pendant la fermentation, et les UFC de L rhamnosus étaient comparable a ceux des témoins (lait écrémé) après production. Après 28 jours, les UFC pour les échantillons supplémentés avec 2 et 3% de farine de lentille étaient aussi élevées que ceux supplémentés avec 1% de lait écrème et les UFC pour les échantillons supplémentés avec 3% de farine de pois étaient plus élevées que ceux de tous les autres échantillons. Durant les 28 jours de production le pH diminue dans les laits probiotiques contenant de la farine de lentille de 4,50 à 3,90 et pour ceux contenant de la farine de pois de 4,50 à 4,04. La synérèse dans laits probiotiques avec 1 à 3% de farine de lentilles ou de pois a été significativement plus faible que les autres échantillons. Tous les échantillons contenant de farine de lentilles avaient significativement une valeur de L plus bas et des valeurs de b et a plus élevés en comparaison aux échantillons supplémentés en lait écrémé. L'addition de farine de pois a entraîné une modification de couleur b.Les laits probiotiques supplémentés avec 1 a 3 % de farine de lentilles et de pois ont des valeurs de G' et G˝ supérieures aux autres échantillons.
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Lee, Horng Jye, and s3048063@student rmit edu au. "The isolation and characterisation of starches from legume grains and their application in food formulations." RMIT University. Applied Sciences, 2008. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20080806.123415.
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As a major group of agricultural commodities, legume grains are widely grown and consumed globally, but are often utilised in the whole form. With increasing evidence of nutritional benefits, these grains are potentially sources of novel ingredients including starches. Accordingly the objective of this study has been to investigate legume starches, particularly their isolation, characterisation and incorporation into selected food products. Using chickpea, faba bean, lentil and mung bean, extraction procedures for the practical isolation of legume starches have been evaluated. A relatively simple method has been established, involving grain cracking, steeping in a mildly alkaline solution, followed by washing, double blending, double sieving and sedimentation. The starches collected for the four legumes were oven dried and the recoveries ranged between 29 and 38%. Compositional analyses confirming that the isolation procedure gave relatively pure starches and scanning electron microscopy showed that the granules were typically ellipsoidal. Laser particle size analysis showed mono-modal distributions with mean diameters between 19.6 and 23.9µm. X-ray diffractograms of legume starches were of the typical C-type, with variations in the intensities and peak distribution indicating some differences in the crystallinity of the starches. Suitable conditions for the measurement of starch gelatinisation characteristics by differential scanning calorimetry were investigated. When optimised conditions were applied, the temperature of gelatinisation ranged from 58.9 for lentil to 65.7 °C for mung bean with corresponding enthalpy values of 9.2 and 5.7 Jg-1. Hot-stage optical microscopy confirmed gelatinisation patterns. The starch pastes demonstrated opalescence with some variation in the degree of clarity. The pasting and viscosity properties measured by the Rapid Visco-Analyser showed some variation in pasting temperatures and considerable differences in peak readings with faba bean starch having lowest and mung bean the highest with values of 307 and 676 RVA units, respectively. In order to study the incorporation of the legume starches, two Asian food products having starch as an ingredient, were selected and adapted as model foods. In this context, vermicelli represented a savoury product and coconut cake a sweet product. Vermicelli and coconut cake samples that incorporated chickpea starch were both preferred by most of the panellists over those containing the other legume starches. The overall conclusions are that the starch extraction method adapted in this investigation was a practical approach, producing relatively pure, white starches. The characteristics of the four legume starches showed many similarities, but there were some variations in the properties, indicating that there may be different applications for their incorporation into food formulations. Sensory evaluations confirmed the usefulness of the starches as food ingredients that provide attractive mouthfeel and textural characteristics. Therefore legume starches offer potential as novel food ingredients warranting further evaluation and larger scale feasibility studies.
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Català, Mora Jaume. "Implante de lente de anclaje iridiano para el tratamiento de la ectopia lentis no traumática. Seguimiento a largo plazo en una cohorte de pacientes pediátricos." Doctoral thesis, Universitat Autònoma de Barcelona, 2016. http://hdl.handle.net/10803/399779.
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La ectopia lentis (EL) consiste en un desplazamiento del cristalino idiopático o asociado a diferentes alteraciones sistémicas o metabólicas, incluyendo el síndrome de Marfan, homocistinuria y el síndrome de Weill-Marchesiani. La EL también puede ocurrir en casos de megaloftalmos anterior idiopático o secundario. En estos pacientes se produce una pérdida visual secundaria a la subluxación del cristalino, que puede provocar alta miopía y astigmatismo. Además, el borde del cristalino subluxado podría interferir con el eje visual, induciendo alteraciones visuales que podrían provocar ambliopía. Otras complicaciones potenciales de la EL incluyen luxación del cristalino en el vítreo o en la cámara anterior, donde podría lesionar el endotelio corneal o provocar un bloqueo pupilar con hipertensión ocular por cierre angular.
El manejo de la EL congénita progresiva es controvertido. Se han propuesto múltiples indicaciones y técnicas quirúrgicas. La extracción del cristalino se puede realizar mediante facoaspiración como a través de lensectomía vía pars plana y vitrectomía. Una vez se ha retirado el cristalino, debemos corregir la afaquia mediante gafas, lentes de contacto o con el implante de una lente intraocular (LIO). Existen múltiples tipos de LIO: LIO de cámara posterior con fijación escleral, anillos de Cionni de fijación escleral y LIO sacular, LIO de cámara anterior y LIO de fijación iridiana pre o retropupilar. La elección del tipo de implante en pacientes pediátricos es una decisión compleja, aunque la mayor parte de cirujanos prefieren LIO de fijación iridiana o escleral en este grupo de pacientes.
Hemos llevado a cabo un estudio prospectivo en una cohorte de 21 ojos de 12 pacientes con ectopia lentis severa y AV inferior a 20/63. Se practicó una vitrectomía 23 G vía pars plana, lensectomía, iridectomía e implante de LIO Artisan en la cámara anterior con un enclavamiento vía pars plana. La edad media en el momento de la cirugía fue de 8,0 ± 5.3 años (rango 3-17 años). Se realizó una exploración oftalmológica completa con mejor agudeza visual corregida (MAVC), biomicroscopía, toma de la presión intraocular, estudio de fondo de ojo y recuento endotelial corneal central (DCEC) antes del tratamiento, a los 3 meses de la intervención y cada 6 meses. A los 12 meses de la cirugía se realizó una biomicroscopía ultrasónica.
El seguimiento medio fue de 39,3 ± 13,0 meses. La MAVC (media ± desviación típica) mejoró desde 0,91 ± 0,29 LogMar en el preoperatorio hasta 0,18 ± 0,23 LogMar al final del seguimiento (p < 0.0001). La distancia media entre el endotelio y la superficie anterior de la LIO después de la cirugía fue de 3,11 ± 0,61 mm. La pérdida endotelial en el postoperatorio inmediato fue del 5,04% ± 9,58% con una perdida endotelial anual del 3,16% ± 4,46%. Un paciente sufrió una luxación de la LIO y un desprendimiento de retina tras una contusión ocular severa y fue tratado con vitrectomía, recolocación de LIO y tamponamiento con gas. Otro paciente desarrolló un edema macular quístico y fue tratado con dexametasona intravítrea.
Conclusiones:
La lensectomía vía pars plana con implante de LIO preiridiana con fijación iridiana por vía posterior mejora la visión de manera significativa en niños con ectopia lentis no traumática.
La pérdida endotelial en el postoperatorio inmediato supone un 5.04 ± 9.58 %., seguida de una pérdida endotelial anual del 3.16 ± 4.56 %. Estos valores son similares a los descritos con otras técnicas y no son superiores a los valores esperados en la población pediátrica.
No se ha encontrado relación directa entre la distancia endotelio-LIO y la pérdida endotelial. La pérdida de un 25% de la población corneal endotelial se asocia, sin alcanzar significación estadística, con una menor distancia endotelio-LIO.
Se han observado menos complicaciones que con otras técnicas de enclavamiento de la LIO de fijación iridiana.Ectopia lentis (EL) is a displacement of the crystalline lens that can be idiopathic or in the setting of various systemic and metabolic disorders, including Marfan's syndrome, homocystinuria, and Weill-Marchesani syndrome. EL can also occur in cases of idiopathic or secondary anterior megalophthalmos. Visual impairment in these patients is secondary to crystalline lens subluxation which may cause high myopia and astigmatism. In addition, the border of the subluxated lens can interfere with the visual axis, thus inducing visual disturbances that could lead to amblyopia. Potential complications of ectopia lentis include dislocation of the lens into the vitreous or anterior chamber, where it can damage the corneal endothelium or induce a pupillary block and angle closure ocular hypertension. The management of progressive congenital EL is controversial. A wide variety of indications and lens removal techniques have been proposed. Both phacoaspiration or pars plana lensectomy and vitrectomy can be used for lens extraction. Once the lens is removed, aphakia must be corrected with glasses, contact lens or intraocular lens (IOL) implantation. A variety of IOL types are available, including scleral–fixated posterior chamber IOL, scleral-fixated Cionni ring, in-the-bag IOL, anterior chamber IOL, and anterior or posteriorly implanted iris-claw IOL. The choice of the IOL implant in paediatric patients is highly challenging, although most surgeons prefer iris or scleral-sutured IOL in this patient population. We conducted a prospective cohort study of 21 eyes from 12 patients with severe ectopia lentis and visual acuity < 20/63. All eyes underwent 23-gauge pars plana vitrectomy, lensectomy, iridectomy, and Artisan IOL implantation in the anterior chamber with iris-claw enclavation via pars plana. Mean age at surgery was 8.0 ± 5.3 y.o. (range 3-17 years). A full ophthalmologic examination including best corrected visual acuity (BCVA), biomicroscopy, intraocular pressure (IOP) measurement, fundus evaluation, and central endothelial cell count (cECC) was performed pre-treatment, at 3 months’ post-surgery, and every 6 months thereafter. Ultrasound biomicroscopy was performed 12 months after surgery. Mean follow-up was 39.3 ± 13.0 months. BCVA (mean ± standard deviation) improved from 0.91 ± 0.29 LogMar preoperatively to 0.18 ± 0.23 LogMar at final follow-up (p < 0.0001). Mean distance from the endothelium to the anterior IOL surface after surgery was 3.11 ± 0.61 mm. Postsurgically, cECC loss was 5.04% ± 9.58% with an annual cECC loss rate of 3.16% ± 4.46%. One patient developed IOL dislocation and retinal detachment after severe ocular contusion requiring vitrectomy, IOL refixation, and gas tamponade. Another patient developed cystoid macular oedema, managed with intravitreal dexamethasone. Conclusion: Pars plana lensectomy and anterior iris-claw implant with posterior fixation improves significantly visual acuity in children with non-traumatic severe ectopia lentis. Endothelial cell loss after surgery achieves a 5,04 ± 9,58 % followed by an annual endothelial cell loss of 3,16 ± 4,56 %. These data are similar to other techniques and they are not higher of what should be expected in the paediatric population. It hasn’t been found a direct relationship between the distance between central corneal endothelium and anterior IOL surface The loss of 25% of the central corneal endothelial population is related, without reaching a statistical significance, to lower endothelium-IOL distances. We have found a lower rate of complications compared to other iris IOL fixation techniques.
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Saha, Gopesh Chandra. "Mapping of foliar disease resistance genes and genes for agro-morphological traits in Lens culinaris Medik." Pullman, Wash. : Washington State University, 2009. http://www.dissertations.wsu.edu/Dissertations/Fall2009/g_saha_112409.pdf.
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Coppa, Isabel Patricia Maria, and Isabel coppa@csw com au. "The use of remote sensing data for broad acre grain crop monitoring in Southeast Australia." RMIT University. Mathematical and Geospatial Sciences, 2006. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20070201.095831.
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In 2025, there will be almost 8 billion people to feed as the worlds population rapidly increases. To meet domestic and export demands, Australian grain productivity needs to approximately triple in the next 20 years, and this production needs to occur in an environmentally sustainable manner. The advent of Hi-tech Precision Farming in Australia has shown promise in recent time to optimize the use of resources. Most
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Koul, Anju L. "Developmental aspects of phenolic compounds in lentils." Thesis, University of Ottawa (Canada), 1987. http://hdl.handle.net/10393/22412.
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Carranza, Cunya Janeth, Nieto Antuane Flórez, Estrella José Málaga, and Castro Andrea Torres. "Lentes Express." Bachelor's thesis, Universidad Peruana de Ciencias Aplicadas (UPC), 2018. http://hdl.handle.net/10757/625278.
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El presente proyecto es una de las propuestas que beneficia a muchas personas. A lo largo de estas semanas, hemos visto diferentes perspectivas de los problemas que las personas plantean cuando quieren optar por un servicio eficiente y confiable. La ciudad en la que vivimos hace que cada vez más personas acudan a un servicio de entrega y que las empresas estén a la vanguardia de la tecnología y la necesidad de los clientes. Somos una empresa que realiza la entrega de lentes de varias clínicas oftalmológicas, que tienen nuestros principales clientes. Esta idea de negocio tiene la necesidad de tener las clínicas. Las soluciones oftalmológicas de potencia proporcionan las lentes para que sus pacientes eviten los problemas logísticos en la clínica, reducen el tiempo y el costo del almacenamiento de los artículos visuales que permanecen en el área de envío. . Además, tomar un servicio de entrega de lentes hace que la clínica sea un valor agregado. De esta manera evita a sus pacientes en la pérdida de tiempo por el tráfico. Tenemos nuestros asesores de ventas que sirven como enlace para captar nuestro objetivo principal, las clínicas oftalmológicas, que desean utilizar nuestro servicio de entrega de lentes. Nuestra distribución se realiza a través de diferentes puntos de Lima que externalizan el servicio de motorizado a una empresa que tiene las aplicaciones roja y móvil. Licencias de servicio, tiempo de espera y tiempo de entrega.The present project is one of the proposals that benefits many people. Throughout these weeks we have seen different perspectives of problems that people bring when they want to opt for an efficient and reliable service. The city in which we live makes more and more people come to a delivery service and that companies are at the forefront of technology and the need of customers. We are a company that performs the delivery of lenses from various ophthalmological clinics, who have our main clients. This business idea has the need to have the clinics. The ophthalmologic solutions of power provide the lenses for their patients to avoid the logistical problems in the clinic, they reduce the time and the cost of the storage of the visual articles that remain in the area of dispatch. . Also, taking a lens delivery service makes the clinic an added value. In this way he avoids his patients in the loss of time by traffic. We have our sales consultants who serve as a link to capture our main objective, ophthalmological clinics, who wish to take our lens delivery service. Our distribution is made by different points of Lima that outsource the service of motorized to a company that has the red and mobile applications. Service licenses, waiting time and delivery time.
Trabajo de investigación
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Sánchez, Alonso Carles. "Dark energy properties from the combination of large-scale structure and weak gravitational lensing in the dark energy survey." Doctoral thesis, Universitat Autònoma de Barcelona, 2017. http://hdl.handle.net/10803/457878.
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Aquesta tesi doctoral es centra en l’extracció d’informació cosmològica utilitzant cartografiats de galàxies fotomètrics, en particular el Dark Energy Survey (DES), tot minimitzant l’impacte dels efectes sistemàtics predominants. La tesi inclou treball tant en les fases inicials com finals de l’anàlisi de dades de DES, des del càlcul de desplaçaments cap al vermell (redshifts) fotomètrics i tests als possibles efectes sistemàtics en la mesura de formes de galàxies, a l’estimació de paràmetres cosmològics utilitzant la combinació d’informació d’estructura a gran escala i lents gravitacionals febles.
A la tesi es presenta la primera demostració de la fiabilitat dels redshifts fotomètrics obtinguts amb la càmera de DES, utilitzant un conjunt nombrós d’algoritmes i dades del període de validació de la ciència de DES (Science Verification, SV) conjuntament amb dades de cartografiats espectroscòpics com ara VVDS, zCOSMOS i ACES. En aquesta part presentem una àmplia comparació de mètodes de càlcul de redshifts fotomètrics utilitzant dades reals de DES.
Respecte a lents gravitacionals febles, a la tesi es caracteritza la senyal al voltant de galàxies per una varietat de mostres, incloent testatge exhaustiu d’efectes sistemàtics i l'estudi del biaix de galàxies, el qual descriu com les galàxies poblen halos de matèria fosca. A part de l'ús de galàxies com a lents, també s’estudia la senyal de lent gravitacional produïda per buits còsmics, per primer cop en un cartografiat fotomètric, utilitzant un nou algoritme per trobar buits que s’ha dissenyat en aquesta tesi concretament per tals conjunts de dades, i que s’ha aplicat aplicar també a l'estudi de l’efecte anomenat Sachs-Wolfe.
Com a peça central de la tesi, es presenten les mesures de correlacions angulars de galàxies i de lents gravitacionals febles al voltant de galàxies vermelles lluminoses en el conjunt de dades DES-SV i es combinen per produir el primer anàlisi cosmològic de la combinació d’estructura a gran escala i lents gravitacionals a DES. Els resultats no són competitius amb els del satèl·lit Planck (DES-SV comprèn només aproximadament 3% de la mostra de dades final de DES) però és consistent amb anàlisis independents amb les mateixes dades i estableix un marc en el modelat i tractament d'errors sistemàtics per combinacions futures de mesures a DES i a la generació pròxima de cartografiats de de galàxies com LSST, WFIRST i Euclid.This PhD thesis is focused on extracting cosmological information from photometric galaxy surveys, in particular the Dark Energy Survey, while minimizing the impact of dominant systematic errors. The thesis includes work on both the first and last stages of survey data analysis, from photometric redshifts (photo-z’s) and shear systematics testing to cosmological parameter estimation using large-scale structure (LSS) and weak gravitational lensing (WL). I performed the first assessment of the photo-z capabilities of the DES Camera by using an extensive set of photo-z algorithms, and data from the DES Science Verification (SV) period matched to spectroscopic data from surveys like VVDS, zCOSMOS and ACES. That part provides a thorough comparison between training and template-based photo-z methods using real data. On WL, I characterized the galaxy-galaxy lensing signal for a variety of galaxy samples, including exhaustive systematics testing and the study of the galaxy bias, which describes the way galaxies populate dark matter halos. Beyond the usage of galaxies as lenses, I also studied the lensing signal produced by cosmic voids, for the first time in a photometric survey, using a new void finder I designed specifically for such data sets, and which has been applied as well to the study of the Integrated Sachs-Wolfe effect. As a major piece of work in the thesis, I measured the angular galaxy clustering and galaxy-galaxy lensing of luminous red galaxies in the DES-SV sample and combined them to conduct the first cosmological analysis from the combination of LSS and WL probes in DES. The results are not competitive with those from Planck (DES-SV comprises only about 3% of the final DES data sample) but show consistency with the DES cosmic shear analysis and establish a benchmark in the modeling and treatment of systematic errors for future combinations of probes in DES and the next generation of galaxy surveys like LSST, WFIRST and Euclid.
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Hanson, Matthew G. "The effect of lentils (Lens culinaris) on hypertension and hypertension–associated vascular remodeling in the spontaneously hypertensive rat." Cambridge University Press, 2013. http://hdl.handle.net/1993/22140.
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Consumption of pulse crops has been linked to improvements in blood vessel function, but which pulse variety/type is better and how they work remains unknown. Two distinct studies were conducted to address these questions.
In study 1, spontaneously hypertensive rats (SHR) were fed control diet, or diets containing 30% (w/w) beans, peas, lentils, chickpeas, or mixed pulses. Normotensive Wistar-Kyoto (WKY) rats were fed the control diet. The lentil–containing diet significantly attenuated the rise in BP (+8 and +31 mmHg, vs. control) and reduced large artery remodeling compared to control. In study 2, SHR were fed control, mixed lentil, green lentil, or red lentil diets for 8 weeks. WKY were fed control or mixed lentil diets. Green lentils decreased arterial remodeling and improved arterial compliance in the SHR.
A diet high in green lentils could be a valuable adjunct to allopathic medicine to mitigate effects of hypertension on the vasculature.October 2013
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Ruhl, Nathan A. "The Lotic-Lentic Gradient in Reservoirs and Estuaries." Ohio University / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1339177201.
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Camboim, Denise Cruz. "Lentigo maligno microinvasivo." Botucatu, 2016. http://hdl.handle.net/11449/143121.
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Orientador: Mariângela Esther Alencar MarquesResumo: O uso da imuno-histoquímica pode aumentar a acurácia na detecção de melanócitos neoplásicos na derme. Com o objetivo de pesquisar microinvasão, cento e nove casos previamente diagnosticados como lentigo maligno (LM) foram resgatados dos arquivos do Departamento de Patologia da Faculdade de Medicina de Botucatu, da Universidade Estadual Paulista (FMB/UNESP) no período de 01/01/2002 a 01/01/2014. As lâminas histológicas de todos os casos foram revisadas pelos autores para confirmação do diagnóstico e seleção do bloco mais representativo para realização de estudo imuno-histoquímico com Melan-A e MITF. Em 25 casos (22,9%) foi observada invasão focal da derme por melanócitos neoplásicos claramente imunomarcados pelo Melan-A. Nos locais onde se evidenciou invasão foi realizada a medida da espessura de Breslow que variou de 0,1 a 0,45 mm. A coloração imuno-histoquímica com MITF evidenciou positividade focal na derme, porém a identificação das células coradas não permitiu a mesma segurança da coloração pelo Melan-A. Todas as lâminas de imuno-histoquímica foram contracoradas pelo Giemsa para diferenciar positividade para o anticorpo testado e melanina. O presente estudo permitiu identificar microinvasão dérmica em 22,9% dos casos de lentigo maligno, mostrando a possibilidade de estadiamento e tratamento inadequado quando utilizada a técnica de rotina. Os achados são um alerta para os patologistas e clínicos, especialmente em lesões de grandes dimensões e associadas com infiltrado linf... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The use of immunohistochemistry can enhance the accuracy in detecting neoplastic melanocytes in the dermis. In order to search for microinvasion, one hundred and nine cases previously diagnosed as lentigo maligna (LM) were retrieved from the archives of the Department of Pathology, Faculty of Medicine of Botucatu, Universidade Estadual Paulista (FMB / UNESP) in the period of 01/01 / 2002 to 01/01/2014. The histological slides of all cases were reviewed by the authors to confirm the diagnosis and selection of the most representative block for performing immunohistochemical study with Melan-A, and MITF. In 25 cases (22.9%) was observed focal dermal invasion by neoplastic melanocytes clearly immunostained for Melan-A. In these cases the Breslow thickness ranged between 0.1 and 0.45mm. Immunohistochemical staining showed MITF focal positivity in the dermis, but did not allow the same certainty of Melan-A staining. In order to distinguish melanin in macrophage cytoplasm from brown-staining melanocytes, the slides were counterstained by Giemsa. This study identified dermal microinvasion in 22.9% cases of lentigo maligna, showing the possibility of inadequate staging and treatment when using the routine technique. The findings are a warning for pathologists and clinicians, especially in large lesions and associated with lymphocytic infiltrate that obscures their limits.
Doutor
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Muzy, Ricardo Casarini. "Através das lentes." reponame:Repositório Institucional da UFSC, 2012. http://repositorio.ufsc.br/handle/123456789/103443.
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Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências da Educação, Programa de Pós-Graduação em Educação, Florianópolis, 2012Made available in DSpace on 2013-07-16T04:39:48Z (GMT). No. of bitstreams: 1 317401.pdf: 1715376 bytes, checksum: 63cd29ecd08cce0430487b8208eaea8c (MD5)
A proposta é investigar as possibilidades do uso da fotografia como instrumento em processos educativos de crianças e jovens. Foram investigadas as possibilidades do uso da fotografia como instrumento educativo e elemento de construção das crianças e jovens envolvidos no processo. A pesquisa se constitui em uma experiência de uma investigação-intervenção, na qual foram realizadas oficinas de fotografia com crianças dos anos iniciais do Ensino Fundamental de uma escola pública na cidade de Florianópolis e com dois grupos de jovens de 17 a 29 anos, de duas diferentes regiões do Estado de Santa Catarina. Estes jovens estavam envolvidos em um projeto de uma entidade de assistência. O trabalho está ancorado e se estrutura principalmente nas propostas da Pedagogia do Oprimido, de Paulo Freire, Filosofia da Libertação, de Enrique Dussel, Jornalismo Libertador, de Elaine Tavares, na Pedagogia de Simón Rodríguez e ainda, nos pressupostos da mídia-educação. Os resultados indicaram que, tanto no ambiente escolar, como no espaço de formação assistencial, a fotografia não se constituía em ferramenta de captura e análise da realidade. As análises indicam ainda, que a fotografia pode ser um poderoso instrumento no processo educativo de crianças e jovens. Compreender esse fazer e dele se apropriar pode também transformar a relação na sala de aula, aproximando o educador da realidade concreta do educando, fazendo com que o processo educativo aconteça de forma fluida e natural.
Abstract : The proposal is to investigate the possibilities of using photography as a tool in the educational process of children and youth. We investigated the possibilities of using photography as a tool for education and development of children and young people involved in the process. The work constitute an experience of a research-intervention, which were conducted photography workshops with children that study in the early years of elementary school at a public school in the city of Florianópolis and with two groups of young people aged 17 to 29 years. These young people were involved in a project of social care. The work is anchored mainly on the structure and motions of Pedagogy of the Oppressed, Paulo Freire's Philosophy of Liberation by Enrique Dussel, Liberator Journalism , Elaine Tavares, in the Pedagogy of Simón Rodríguez and also in the assumptions of media educational. The results indicated that both the school setting, as space training assistance, the photograph does not constitute tool for capturing and analyzing reality. The analyzes also indicate, that photography can be a powerful tool in the educational process of children and youth. We understand that the use of fotografe can transform the relationship in the classroom. Can the teacher approaching the reality of the student, making the educational process happens smoothly and naturally.
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JR, MARCOS AURELIO PINTO MARZANO. "ROBOTIC SYSTEM FOR MONITORING WATER QUALITY IN LENTIC ENVIRONMENTS." PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO, 2016. http://www.maxwell.vrac.puc-rio.br/Busca_etds.php?strSecao=resultado&nrSeq=36947@1.
Full textAbstract:
PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIROCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO
Nas últimas décadas, a crescente conscientização ambiental levou ao reconhecimento da necessidade do uso responsável dos recursos hídricos. Para garantir isso, a boa gestão de reservatórios hídricos requer um monitoramento ambiental adequado, com medições confiáveis dos parâmetros de qualidade da água em vários pontos do reservatório, permitindo o controle da qualidade da água e seus impactos na fauna, flora e comunidades ribeirinhas dos reservatórios. O monitoramento das variáveis ambientais dos reservatórios é atualmente realizado por processo tradicional de coleta manual. Infelizmente, no Brasil, as iniciativas de produzir um sistema robótico aquático com tecnologia nacional e de baixo custo, quando comparado a equivalentes importados, são ainda raras e se restringem a algumas poucas instituições acadêmicas, não tendo sido localizado nenhum fabricante comercial deste tipo de veículo no país. Visando preencher esta lacuna, o presente trabalho teve como objetivo o desenvolvimento do protótipo de um sistema robótico aquático capaz de se locomover autonomamente em lagoas, lagos e reservatórios, coletando informações físico-químicas da água e armazenando estes dados na memória. Além disso, foi incluído no protótipo uma câmera de vídeo, sistema de iluminação e um sistema de controle remoto, objetivando o controle pela equipe em terra. Nos testes realizados em dias ensolarados e chuvosos, o robô apresentou boa dirigibilidade, estabilidade e manobrabilidade. O vaso de pressão do sistema robótico resistiu às pressões necessárias durante os testes, a eletrônica conseguiu atender as especificações de projeto e o software conseguiu estabelecer um controle de navegação, cumprindo o trajeto de uma rota estabelecida.
In recent decades, the growing environmental awareness has led to the recognition of the need for responsible use of water resources. To ensure this, the good management of water reservoirs requires adequate environmental monitoring, with reliable measurements of water quality parameters in various parts of the reservoir, allowing the control of water quality and its impacts on fauna, flora and riverine communities of the reservoirs. Monitoring environmental variables of the reservoirs is currently performed by traditional process of manual collection. Unfortunately, in Brazil, initiatives to produce a water robotic system with national and low cost technology, compared to imported equivalents, are still rare and restricted to a few academic institutions, and no commercial manufacturer of this type of vehicle was found in the country. Aiming to fill this gap, the main objective of this study was to develop a prototype of a water robotic system capable of autonomously navigate in ponds, lakes and reservoirs, collecting physicochemical information of water and storing this data in memory. Moreover, a video camera, illumination and a remote control system were included in the prototype, allowing the team on the ground to control the prototype. In tests conducted in sunny and rainy days, the robot presented good handling, stability and maneuverability. The robotic system pressure vessel resisted pressures required during testing, the electronics met the design specifications and the software was able to establish a navigation control, fulfilling the path of an established route.
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Rosset, Véronique, Albert Ruhi, Michael T. Bogan, and Thibault Datry. "Do lentic and lotic communities respond similarly to drying?" WILEY, 2017. http://hdl.handle.net/10150/625362.
Full textAbstract:
Disturbance is a central factor shaping composition, structure, and dynamics of local communities. Drying is a disturbance that occurs in aquatic ecosystems globally and can strongly influence their communities. Although the effects of drying may depend on ecosystem connectivity and the dispersal abilities of resident species, there have been no comparisons of community responses to drying between lentic and lotic ecosystems across different climates. Here, we predicted that drying would have stronger effects on aquatic communities in isolated lentic ecosystems than in dendritic lotic ecosystems, owing to the higher hydrological connectivity of the latter, and that drying would have stronger effects on passive than on active dispersers, because of the potentially higher recolonizing ability of the latter. We tested these predictions by comparing alpha diversity, phylogenetic relatedness, and beta diversity for active and passive dispersers, in both ecosystem types across five climatic regions. Drying caused greater declines in alpha diversity in lentic than in lotic ecosystems. Communities that experienced drying were more similar to one another than those of perennial sites, and this pattern was especially pronounced in lentic ecosystems. In contrast, drying did not influence the contributions of turnover and richness gradients to beta diversity. Additionally, dispersal mode did not influence community responses to drying. Relatively weaker effects of drying in lotic compared to lentic systems were likely due to the hydrological connectivity among perennial and temporary river sites, which may facilitate dispersal of organisms to escape drying and recolonize rewetted sites. Collectively, our results suggest that habitat connectivity may ameliorate (and fragmentation may worsen) the impacts of drying disturbance. This is an important finding in light of increasing drying and concomitant aquatic habitat fragmentation under global change.