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Journal articles on the topic 'LED-induced fluorescence'

Author: Grafiati

Published: 25 May 2024

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1

Geng, Xuhui, and Yafeng Guan. "Research highlight on CJAC—LED induced fluorescence detector." Chinese Journal of Analytical Chemistry 50, no. 5 (May 2022): 100084. http://dx.doi.org/10.1016/j.cjac.2022.100084.

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2

Gu, Wenwen, Jie Huang, and Weimin Tan. "LED induced fluorescence detector integrated in microfluidic cell chip." International Journal of Nanotechnology 12, no. 10/11/12 (2015): 742. http://dx.doi.org/10.1504/ijnt.2015.071785.

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3

Qi, Xiaoguang, Xianglong Hao, Muzi Zhang, Lili Jiang, Wenyue Gao, and Chi Wu. "Extensible LED-Induced Integrated Fluorescence Detection Module for Quantitative Analysis of Lucigenin Concentration." Photonics 10, no. 4 (April 1, 2023): 392. http://dx.doi.org/10.3390/photonics10040392.

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We developed an extensible LED-induced fluorescence detection module with a highly integrated and ultra-compact structure. A target-oriented design methodology was used to demonstrate the module’s optimal design. Lucigenin solution was used as a test sample in evaluation trials to demonstrate the module’s quantitative fluorescence detection capability. Results showed that the integrated module has an outstanding linear response in the range of 0–1 μmol·L−1, with sensitivity and limit of detection (LOD) of 0.1692 V/μmol·L−1 and 0.03 μmol·L−1, respectively. Statistical analyses showed that our integrated module has extremely high repeatability and accuracy, i.e., the values of Pearson’s correlation coefficient and root-mean-square error exceeded 0.9995 and 1.8‰, respectively. More importantly, the integrated module possesses favorable extensibility and can realize on-demand rapid fluorescence-signal detection of other targets using appropriate parameter combinations. This module offers new opportunities for reliable, cost-effective and easy-to-use fluorescence-signal detection, especially in resource-constrained fluorescence detection applications.

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4

Mukunda, Darshan Chikkanayakanahalli, Jackson Rodrigues, Vijay Kumar Joshi, Chandavalli Ramappa Raghushaker, and Krishna Kishore Mahato. "A comprehensive review on LED-induced fluorescence in diagnostic pathology." Biosensors and Bioelectronics 209 (August 2022): 114230. http://dx.doi.org/10.1016/j.bios.2022.114230.

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5

GU Wen-wen, 顾雯雯. "LED induced transmitted fluorescence detector integrated in microfluidic cell chip." Optics and Precision Engineering 22, no. 8 (2014): 2159–65. http://dx.doi.org/10.3788/ope.20142208.2159.

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6

Mustafic, Adnan, Changying Li, and Mark Haidekker. "Blue and UV LED-induced fluorescence in cotton foreign matter." Journal of Biological Engineering 8, no. 1 (2014): 29. http://dx.doi.org/10.1186/1754-1611-8-29.

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7

Dong, Yongjiang, Xuan Liu, Liang Mei, Chao Feng, Chunsheng Yan, and Sailing He. "LED-induced fluorescence system for tea classification and quality assessment." Journal of Food Engineering 137 (September 2014): 95–100. http://dx.doi.org/10.1016/j.jfoodeng.2014.03.027.

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8

Gao, Fei, Yongjiang Dong, Weimin Xiao, Bin Yin, Chunsheng Yan, and Sailing He. "LED-induced fluorescence spectroscopy technique for apple freshness and quality detection." Postharvest Biology and Technology 119 (September 2016): 27–32. http://dx.doi.org/10.1016/j.postharvbio.2016.04.020.

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9

Rodat-Boutonnet, Audrey, Pierre Naccache, Arnaud Morin, Jacques Fabre, Bernard Feurer, and François Couderc. "A comparative study of LED-induced fluorescence and laser-induced fluorescence in SDS-CGE: Application to the analysis of antibodies." ELECTROPHORESIS 33, no. 12 (June 28, 2012): 1709–14. http://dx.doi.org/10.1002/elps.201200132.

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10

Grochocki, Wojciech, Magdalena Buszewska-Forajta, Szymon Macioszek, and Michał J. Markuszewski. "Determination of Urinary Pterins by Capillary Electrophoresis Coupled with LED-Induced Fluorescence Detector." Molecules 24, no. 6 (March 24, 2019): 1166. http://dx.doi.org/10.3390/molecules24061166.

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Urinary pterins have been found as potential biomarkers in many pathophysiological conditions including inflammation, viral infections, and cancer. However, pterins determination in biological samples is difficult due to their degradation under exposure to air, light, and heat. Besides, they occur at shallow concentration levels, and thus, standard UV detectors cannot be used without additional sample preconcentration. On the other hand, ultra-sensitive laser-induced fluorescence (LIF) detection can be used since pterins exhibit native fluorescence. The main factor that limits an everyday use of LIF detectors is its high price. Here, an alternative detector, i.e., light-emitted diode induced fluorescence (LEDIF) detector, was evaluated for the determination of pterins in urine samples after capillary electrophoresis (CE) separation. An optimized method was validated in terms of linearity range, limit of detection (LOD), limit of quantification (LOQ), intra- and interday precision and accuracy, sample stability in the autosampler, and sample stability during the freezing/thawing cycle. The obtained LOD (0.1 µM) and LOQ (0.3 µM) values were three-order of magnitude lower compared to UV detector, and two orders of magnitude higher compared to previously reported house-built LIF detector. The applicability of the validated method was demonstrated in the analysis of urine samples from healthy individuals and cancer patients.

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11

Lin, Li, Lu, Sun, Chen, Wei, and Ming. "Robust Classification of Tea Based on Multi-Channel LED-Induced Fluorescence and a Convolutional Neural Network." Sensors 19, no. 21 (October 28, 2019): 4687. http://dx.doi.org/10.3390/s19214687.

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A multi-channel light emitting diode (LED)-induced fluorescence system combined with a convolutional neural network (CNN) analytical method was proposed to classify the varieties of tea leaves. The fluorescence system was developed employing seven LEDs with spectra ranging from ultra-violet (UV) to blue as excitation light sources. The LEDs were lit up sequentially to induce a respective fluorescence spectrum, and their ability to excite fluorescence from components in tea leaves were investigated. All the spectral data were merged together to form a two-dimensional matrix and processed by a CNN model, which is famous for its strong ability in pattern recognition. Principal component analysis combined with k-nearest-neighbor classification was also employed as a baseline for comparison. Six grades of green tea, two types of black tea and one kind of white tea were verified. The result proved a significant improvement in accuracy and showed that the proposed system and methodology provides a fast, compact and robust approach for tea classification.

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12

Qin, Y. L., X. L. Luan, L. J. Bi, Z. Lü, Y. Q. Sheng, G. Somesfalean, C. N. Zhou, and Z. G. Zhang. "Real-time detection of dental calculus by blue-LED-induced fluorescence spectroscopy." Journal of Photochemistry and Photobiology B: Biology 87, no. 2 (May 2007): 88–94. http://dx.doi.org/10.1016/j.jphotobiol.2007.03.002.

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13

Miyaki, Kyo, Yanli Guo, Takuya Shimosaka, Tatsuro Nakagama, Hizuru Nakajima, and Katsumi Uchiyama. "Fabrication of an integrated PDMS microchip incorporating an LED-induced fluorescence device." Analytical and Bioanalytical Chemistry 382, no. 3 (May 10, 2005): 810–16. http://dx.doi.org/10.1007/s00216-004-3015-1.

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14

Xue, Shuhua, Katsumi Uchiyama, and Hai-fang Li. "Determination of ammonium on an integrated microchip with LED-induced fluorescence detection." Journal of Environmental Sciences 24, no. 3 (March 2012): 564–70. http://dx.doi.org/10.1016/s1001-0742(11)60802-4.

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15

Moreira, Cristian M., Evelyn Marín-Barroso, Sirley V. Pereira, Julio Raba, Germán A. Messina, and Franco A. Bertolino. "A nanostructured paper-based device for phenylalanine neonatal screening by LED-induced fluorescence." Analytical Methods 12, no. 12 (2020): 1624–30. http://dx.doi.org/10.1039/c9ay02774b.

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In this work, a novel paper-based analytical device (PAD) coupled with LED-induced fluorescence (LIF) detection (fPAD) for the rapid, selective, and sensitive quantification of phenylalanine (Phe) in neonatal samples was developed.

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16

Zhang, Ting, Yuyang Liu, Zhuoping Dai, Lihan Cui, Hongze Lin, Zejian Li, Kaihua Wu, and Guangyu Liu. "Quantitative Detection of Extra Virgin Olive Oil Adulteration, as Opposed to Peanut and Soybean Oil, Employing LED-Induced Fluorescence Spectroscopy." Sensors 22, no. 3 (February 6, 2022): 1227. http://dx.doi.org/10.3390/s22031227.

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As it is high in value, extra virgin olive oil (EVOO) is frequently blended with inferior vegetable oils. This study presents an optical method for determining the adulteration level of EVOO with soybean oil as well as peanut oil using LED-induced fluorescence spectroscopy. Eight LEDs with central wavelengths from ultra-violet (UV) to blue are tested to induce the fluorescence spectra of EVOO, peanut oil, and soybean oil, and the UV LED of 372 nm is selected for further detection. Samples are prepared by mixing olive oil with different volume fractions of peanut or soybean oil, and their fluorescence spectra are collected. Different pre-processing and regression methods are utilized to build the prediction model, and good linearity is obtained between the predicted and actual adulteration concentration. This result, accompanied by the non-destruction and no pre-treatment characteristics, proves that it is feasible to use LED-induced fluorescence spectroscopy as a way to investigate the EVOO adulteration level, and paves the way for building a hand-hold device that can be applied to real market conditions in the future.

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17

Liu Liangchen, 刘梁晨, 杨瑞芳 Yang Ruifang, 赵南京 Zhao Nanjing, 石高勇 Shi Gaoyong, 杨金强 Yang Jinqiang, 黄朋 Huang Peng, 殷高方 Yin Gaofang, 方丽 Fang Li, 刘建国 Liu Jianguo, and 刘文清 Liu Wenqing. "LED诱导荧光的土壤多环芳烃快速检测方法." Acta Optica Sinica 43, no. 18 (2023): 1812003. http://dx.doi.org/10.3788/aos222067.

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18

Lee, Seon Min, Kicheol Yoon, Sangyun Lee, Seung Yeob Ryu, and Kwang Gi Kim. "Multi-Asymmetric Irradiation Method Using a Ring Array to Obtain an Emission-Capable LED Beam Power Effect to Observe Cancer Removal Status in a Surgical Microscope." Diagnostics 13, no. 22 (November 20, 2023): 3482. http://dx.doi.org/10.3390/diagnostics13223482.

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The light emitting diodes (LEDs) used in surgical fluorescence microscopes have weak power, to induce fluorescence emission. The LED induces fluorescence emission throughout a lesion due to its large beam width; however, the beam irradiation intensity is not uniform within the beam width, resulting in a fluorescence emission induction difference. To overcome this problem, this study proposes an asymmetric irradiation array for supplying power uniformly throughout the beam width of the LED and increasing the intensity of the LED. To increase the irradiation power of the LEDs, a multi-asymmetric irradiation method with a ring-type array structure was used. The LED consisted of eight rings, and the space between the LEDs, the placement position, and the placement angle were analyzed to devise an experimental method using 3D printing technology. To test the irradiation power of the LED, the working distance (WD) between the LED and target was 30 cm. The bias voltage of the LED for irradiating the light source was 5.0 V and the measured power was 4.63 mW. The brightness (lux) was 1153 lx. Consequently, the LED satisfied the fluorescence emission induction conditions. The diameter of the LED-irradiated area was 9.5 cm. Therefore, this LED could be used to observe fluorescent emission-guided lesions. This study maximized the advantages of LEDs with optimal conditions for fluorescence emission by increasing the beam width, irradiation area, and energy efficiency, using a small number of LEDs at the maximum WD. The proposed method, optimized for fluorescence expression-induced surgery, can be made available at clinical sites by mass producing them through semiconductor processes.

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19

Vladev, Veselin, Mariya Brazkova, Stefan Bozhkov, Galena Angelova, Denica Blazheva, Stefka Minkova, Krastena Nikolova, and Tinko Eftimov. "Light-Emitting-Diode-Induced Fluorescence from Organic Dyes for Application in Excitation–Emission Fluorescence Spectroscopy for Food System Analysis." Foods 13, no. 9 (April 26, 2024): 1329. http://dx.doi.org/10.3390/foods13091329.

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An experimental study is presented on the possibility of using the fluorescence from organic dyes as a broadband light source together with a monochromator for applications in excitation–emission matrix (EEM) fluorescence spectroscopy. A high-power single-chip light-emitting diode (LED) was chosen as an excitation source with a central output wavelength at 365 nm to excite a fluorescent solution of Coumarin 1 dye dissolved in ethanol. Two excitation configurations were investigated: direct excitation from the LED and excitation through an optical-fiber-coupled LED. A Czerny–Turner monochromator with a diffraction grating was used for the spectral tuning of the fluorescence. A simple method was investigated for increasing the efficiency of the excitation as well as the fluorescence signal collection by using a diffuse reflector composed of barium sulfate (BaSO4) and polyvinyl alcohol (PVA). As research objects, extra-virgin olive oil (EVOO), Coumarin 6 dye, and Perylene, a polycyclic aromatic hydrocarbon (PAH), were used. The results showed that the light-emitting-diode-induced fluorescence was sufficient to cover the losses on the optical path to the monochromator output, where a detectable signal could be obtained. The obtained results reveal the practical possibility of applying the fluorescence from dyes as a light source for food system analysis by EEM fluorescence spectroscopy.

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20

Buah-Bassuah, Paul K., Hubertus M. von Bergmann, Ebenezer T. Tatchie, and Christine M. Steenkamp. "A portable fibre-probe ultraviolet light emitting diode (LED)-induced fluorescence detection system." Measurement Science and Technology 19, no. 2 (January 14, 2008): 025601. http://dx.doi.org/10.1088/0957-0233/19/2/025601.

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21

Ric, Audrey, Vincent Ecochard, Jason S. Iacovoni, Audrey Boutonnet, Frédéric Ginot, Varravaddheay Ong-Meang, Véréna Poinsot, Laurent Paquereau, and François Couderc. "G-quadruplex aptamer selection using capillary electrophoresis-LED-induced fluorescence and Illumina sequencing." Analytical and Bioanalytical Chemistry 410, no. 7 (January 29, 2018): 1991–2000. http://dx.doi.org/10.1007/s00216-018-0865-5.

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22

Su, Ying, Qinghui Zeng, Xuejiao Chen, Weiguang Ye, Lushuang She, Ximing Gao, Zhongyuan Ren, and Xiaomeng Li. "Highly efficient CsPbBr3 perovskite nanocrystals induced by structure transformation between CsPbBr3 and Cs4PbBr6 phases." Journal of Materials Chemistry C 7, no. 25 (2019): 7548–53. http://dx.doi.org/10.1039/c9tc01763a.

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23

Mustafic, Adnan, Erin E. Roberts, Michael D. Toews, and Mark A. Haidekker. "LED-Induced fluorescence and image analysis to detect stink bug damage in cotton bolls." Journal of Biological Engineering 7, no. 1 (2013): 5. http://dx.doi.org/10.1186/1754-1611-7-5.

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24

J. Qin, K. Chao, M. S. Kim, S. Kang, B.-K. Cho, and W. Jun. "Detection of Organic Residues on Poultry Processing Equipment Surfaces by LED-Induced Fluorescence Imaging." Applied Engineering in Agriculture 27, no. 1 (2011): 153–61. http://dx.doi.org/10.13031/2013.36218.

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25

Abd Halim, Adyani Azizah, Mohammed Suleiman Zaroog, Habsah Abdul Kadir, and Saad Tayyab. "Molten Globule-Like Partially Folded State ofBacillus licheniformis α-Amylase at Low pH Induced by 1,1,1,3,3,3-Hexafluoroisopropanol." Scientific World Journal 2014 (2014): 1–9. http://dx.doi.org/10.1155/2014/824768.

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Effect of 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) on acid-denaturedBacillus licheniformis α-amylase (BLA) at pH 2.0 was investigated by far-UV CD, intrinsic fluorescence, and ANS fluorescence measurements. Addition of increasing HFIP concentrations led to an increase in the mean residue ellipticity at 222 nm (MRE222 nm) up to 1.5 M HFIP concentration beyond which it sloped off. A small increase in the intrinsic fluorescence and a marked increase in the ANS fluorescence were also observed up to 0.4 M HFIP concentration, both of which decreased thereafter. Far- and near-UV CD spectra of the HFIP-induced state observed at 0.4 M HFIP showed significant retention of the secondary structures closer to native BLA but a disordered tertiary structure. Increase in the ANS fluorescence intensity was also observed with the HFIP-induced state, suggesting exposure of the hydrophobic clusters to the solvent. Furthermore, thermal denaturation of HFIP-induced state showed a non-cooperative transition. Taken together, all these results suggested that HFIP-induced state of BLA represented a molten globule-like state at pH 2.0.

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26

Ng, Chee-Loon, Yan-Jie Ng, Qing-Qing Chen, and Harold F. Hemond. "Corrections for matrix effects on fluorescence measurement of a multi-platform optical sensor." Water Practice and Technology 11, no. 3 (September 1, 2016): 644–60. http://dx.doi.org/10.2166/wpt.2016.069.

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The LEDIF (LED-induced fluorescence) is an in situ optical instrument that utilizes fluorescence, absorbance, and scattering to identify and quantify substances in water bodies. In this study, matrix effects on fluorescence signals caused by inner filtering, temperature, intramolecular deactivation, turbidity, and pH were investigated, and compensation equations developed to correct measured values and improve accuracy. Multiple simultaneous matrix effect corrections were demonstrated with a laboratory sample subjected to known interferences and physical conditions. In general, compensation was found to be important to improve the accuracy of fluorescence measurements.

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27

Ritz, Rainer, Guenther C. Feigl, Martin U. Schuhmann, André Ehrhardt, Soeren Danz, Susan Noell, Antje Bornemann, and Marcos S. Tatagiba. "Use of 5-ALA fluorescence guided endoscopic biopsy of a deep-seated primary malignant brain tumor." Journal of Neurosurgery 114, no. 5 (May 2011): 1410–13. http://dx.doi.org/10.3171/2010.11.jns10250.

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The introduction of fluorescence-guided resection of primary malignant brain tumors was a milestone in neurosurgery. Deep-seated malignant brain tumors are often not approachable for microsurgical resection. For diagnosis and therapy, new strategies are recommended. The combination of endoscopy and 5-aminolevulinic acid–induced protoporphyrin IX (5-ALA-induced Pp IX) fluorescence–guided procedures supported by neuronavigation seems an interesting option. Here the authors report on a combined approach for 5-ALA fluorescence–guided biopsy in which they use an endoscopy system based on an Xe lamp (excitation approximately λ = 407 nm; dichroic filter system λ = 380–430 nm) to treat a malignant tumor of the thalamus and perform a ventriculostomy and septostomy. The excitation filter and emission filter are adapted to ensure that the remaining visible blue remission is sufficient to superimpose on or suppress the excited red fluorescence of the endogenous fluorochromes. The authors report that the lesion was easily detectable in the fluorescence mode and that biopsy led to histological diagnosis.

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28

Park, Jinho, Young-Su Jeong, Hyunwoo Nam, and Kibong Choi. "Signal Encoder of Real-Time Bio-Aerosol Counter Using 280 nm UV-LED Induced Fluorescence." IEEE Sensors Journal 20, no. 22 (November 15, 2020): 13471–79. http://dx.doi.org/10.1109/jsen.2020.3006880.

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29

Dickens, Jason E., Michael S. Vaughn, Mervin Taylor, and Mike Ponstingl. "An LED array-based light induced fluorescence sensor for real-time process and field monitoring." Sensors and Actuators B: Chemical 158, no. 1 (November 2011): 35–42. http://dx.doi.org/10.1016/j.snb.2011.04.077.

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30

Chen, Juan, Hyun Dong Kim, and Kyung Chun Kim. "Measurement of dissolved oxygen diffusion coefficient in a microchannel using UV-LED induced fluorescence method." Microfluidics and Nanofluidics 14, no. 3-4 (November 3, 2012): 541–50. http://dx.doi.org/10.1007/s10404-012-1072-x.

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31

Wang, Shuping, Xinchun Li, Jianping Yang, Xiujuan Yang, Fenghua Hou, and Zuanguang Chen. "Rapid Determination of Creatinine in Human Urine by Microchip Electrophoresis with LED Induced Fluorescence Detection." Chromatographia 75, no. 21-22 (September 23, 2012): 1287–93. http://dx.doi.org/10.1007/s10337-012-2324-3.

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32

Ji, Hongyun, Xueyan Zhang, Feng Yang, Jiali Wang, Hongyan Yuan, and Dan Xiao. "Sensitive determination of l-hydroxyproline in dairy products by capillary electrophoresis with in-capillary optical fiber light-emitting diode-induced fluorescence detection." Analytical Methods 10, no. 19 (2018): 2211–16. http://dx.doi.org/10.1039/c7ay02356a.

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A sensitive capillary electrophoretic method with in-capillary optical fiber light-emitting diode-induced fluorescence detection (ICOF-LED-IFD-CE) was developed for determination of l-Hyp and further for identification of hydrolyzed leather proteins in dairy products.

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33

Adigal, Sphurti S., Sulatha V. Bhandary, Nagaraj Hegde, V. R. Nidheesh, Reena V. John, Alisha Rizvi, Sajan D. George, V. B. Kartha, and Santhosh Chidangil. "Correction: Protein profile analysis of tear fluid with hyphenated HPLC-UV LED-induced fluorescence detection for the diagnosis of dry eye syndrome." RSC Advances 13, no. 39 (2023): 27446. http://dx.doi.org/10.1039/d3ra90084c.

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Correction for ‘Protein profile analysis of tear fluid with hyphenated HPLC-UV LED-induced fluorescence detection for the diagnosis of dry eye syndrome’ by Sphurti S. Adigal et al., RSC Adv., 2023, 13, 22559–22568. https://doi.org/10.1039/D3RA04389D.

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34

Mazina, Jekaterina, Anastassija Spiljova, Merike Vaher, Mihkel Kaljurand, and Maria Kulp. "A rapid capillary electrophoresis method with LED-induced native fluorescence detection for the analysis of cannabinoids in oral fluid." Analytical Methods 7, no. 18 (2015): 7741–47. http://dx.doi.org/10.1039/c5ay01595b.

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In the present study, a capillary electrophoresis method with native fluorescence detection for the quantification of two main marijuana cannabinoids, tetrahydrocannabinol (THC) and cannabidiol (CBD), in oral fluid is described.

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35

Tassery, Hervé, Stephen Koubi, Anne Raskin, Elodie Terrer, Alexandro Dionne, Gauthier Weisrock, Caroline Sarraquigne, and Alain Mazuir. "A New Concept in Restorative Dentistry: LIFEDT— Light-Induced Fluorescence Evaluator for Diagnosis and Treatment: Part 2 – Treatment of Dentinal Caries." Journal of Contemporary Dental Practice 11, no. 1 (January 2010): 95–102. http://dx.doi.org/10.5005/jcdp-11-1-95.

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Abstract Aim A new and innovative therapeutic concept using a light-induced fluorescence evaluator for diagnosis and treatment (LIFEDT) of dental caries based on the imaging and autofluorescence of dental tissues is proposed. The aims of this series of in vivo experiments are to compare and analyze the brightness variations of sound dentin and active and arrested carious dentin illuminated with an intraoral LED camera and to determine if this new device could be helpful in daily practice to discriminate between caries and sound dentin. Methods and Materials A new intraoral LED camera that emits visible blue light was used in this in vivo study to illuminate and photograph 15 teeth at high magnification. The magnified images were examined using the free Image J V® version 1.41 software. Four standardized rectangular areas were drawn on each picture that included both healthy and pathologic areas to analyze variations in brightness using a brightness formula: L* = 0.299 Red + 0.587 Green + 0.114 Blue. Results Statistically significant differences in the brightness were found between active and arrested caries processes in an area of infected dentin designated Z2. Within the limitations of this in vivo study, the images created with the intraoral LED camera revealed significant variations in fluorescence between sound dentin and active and arrested caries processes. Conclusions The LIFEDT concept provides a therapeutic concept based on these findings of variations in fluorescence between healthy and pathologic tissue. Clinical Significance This concept defines a pragmatic clinical and therapeutic approach for treating active and arrested carious lesions based on the interpretation of variations of a fluorescence signal and applying the LIFEDT concept to the treatment of dentin carious lesions. Citation Terrer E, Raskin A, Koubi S, Dionne A, Weisrock G, Sarraquigne C, Mazuir A, Tassery H. A New Concept in Restorative Dentistry: LIFEDT—Light-Induced Fluorescence Evaluator for Diagnosis and Treatment: Part 2 – Treatment of Dentinal Caries. J Contemp Dent Pract [Internet]. 2010 Jan; 11(1):095-102. Available from: http://www.thejcdp.com/journal/view/ volume11-issue1-terrer.

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36

Sandra, Retno Damayanti, Rut Juniar Nainggolan, Mitha Sa’diyah, Anita Sekar Kusumastuti, Siska Ratna Anggraeni, Yusuf Hendrawan, and Ken Abamba Omwange. "Predicting piperine content in javanese long pepper using fluorescence imaging and machine learning model." BIO Web of Conferences 90 (2024): 02003. http://dx.doi.org/10.1051/bioconf/20249002003.

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The conventional method for determining piperine content involves a series of labor-intensive steps, including drying the pepper samples, grinding them, and then extracting them using high-grade ethanol through a reflux method. While effective, this process is time-consuming and resource-intensive, posing limitations in terms of efficiency and the ability to address potential variations. Therefore, there is an urgent need to explore more efficient and rapid approaches for accurately measuring and predicting piperine content, with machine learning approach. This research aims to explore the potential of using fluorescence imaging methods and ANN models to increase the efficiency of measuring piperine content on Javanese long pepper. We propose a machine learning approach using UV-induced fluorescence imaging of Javanese long pepper. UV LEDs (365 nm) induced fluorescence, with color variation indicating piperine content. An artificial neural network (ANN) model, trained on color texture features from fluorescence images, predicted piperine content, achieving an R2 value of 0.88025 with ten selected features using the One-R attribute. The final ANN, configured with 'trainoss' learning, 'tansig' activation, 0.1 learning rate, and 10-40-10 nodes, demonstrated a testing R2 of 0.8943 and MSE of 0.0875. LED-induced fluorescence enhances machine learning's piperine content prediction. This research contributes to more efficient piperine content measurement methods.

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37

Jalink, H., and R. van der Schoor. "LED INDUCED CHLOROPHYLL FLUORESCENCE TRANSIENT IMAGER FOR MEASUREMENTS OF HEALTH AND STRESS STATUS OF WHOLE PLANTS." Acta Horticulturae, no. 893 (April 2011): 307–15. http://dx.doi.org/10.17660/actahortic.2011.893.26.

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38

Silva, E. A., A. S. Gouveia-Neto, R. A. Oliveira, D. S. Moura, P. C. Cunha, E. B. Costa, T. J. R. Câmara, and L. G. Willadino. "Water Deficit and Salt Stress Diagnosis Through LED Induced Chlorophyll Fluorescence Analysis in Jatropha curcas L." Journal of Fluorescence 22, no. 2 (November 4, 2011): 623–30. http://dx.doi.org/10.1007/s10895-011-0998-9.

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39

Martini, Pacheco-Labrador, Perez-Priego, van der Tol, Madany, Julitta, Rossini, et al. "Nitrogen and Phosphorus effect on Sun-Induced Fluorescence and Gross Primary Productivity in Mediterranean Grassland." Remote Sensing 11, no. 21 (October 31, 2019): 2562. http://dx.doi.org/10.3390/rs11212562.

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Sun-Induced fluorescence at 760 nm (F760) is increasingly being used to predict gross primary production (GPP) through light use efficiency (LUE) modeling, even though the mechanistic processes that link the two are not well understood. We analyzed the effect of nitrogen (N) and phosphorous (P) availability on the processes that link GPP and F760 in a Mediterranean grassland manipulated with nutrient addition. To do so, we used a combination of process-based modeling with Soil-Canopy Observation of Photosynthesis and Energy (SCOPE), and statistical analyses such as path modeling. With this study, we uncover the mechanisms that link the fertilization-driven changes in canopy nitrogen concentration (N%) to the observed changes in F760 and GPP. N addition changed plant community structure and increased canopy chlorophyll content, which jointly led to changes in photosynthetic active radiation (APAR), ultimately affecting both GPP and F760. Changes in the abundance of graminoids, (%graminoids) driven by N addition led to changes in structural properties of the canopy such as leaf angle distribution, that ultimately influenced observed F760 by controlling the escape probability of F760 (Fesc). In particular, we found a change in GPP–F760 relationship between the first and the second year of the experiment that was largely driven by the effect of plant type composition on Fesc, whose best predictor is %graminoids. The P addition led to a statistically significant increase on light use efficiency of fluorescence emission (LUEf), in particular in plots also with N addition, consistent with leaf level studies. The N addition induced changes in the biophysical properties of the canopy that led to a trade-off between surface temperature (Ts), which decreased, and F760 at leaf scale (F760leaf,fw), which increased. We found that Ts is an important predictor of the light use efficiency of photosynthesis, indicating the importance of Ts in LUE modeling approaches to predict GPP.

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Casto, Laura D., Kevin B. Do, and Christopher A. Baker. "A Miniature 3D Printed LED-Induced Fluorescence Detector for Capillary Electrophoresis and Dual-Detector Taylor Dispersion Analysis." Analytical Chemistry 91, no. 15 (July 9, 2019): 9451–57. http://dx.doi.org/10.1021/acs.analchem.8b05824.

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Liu, Zhijun, Stanley B. Carpenter, and Roysell J. Constantin. "Camptothecin production in Camptotheca acuminata seedlings in response to shading and flooding." Canadian Journal of Botany 75, no. 2 (February 1, 1997): 368–73. http://dx.doi.org/10.1139/b97-039.

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One-year-old Camptotheca acuminata seedlings were grown under heavy shade (27% full sunlight), slight shade (67% full sunlight), or full sunlight for 2 weeks, then subjected to flooding for 4 weeks under continued shading schemes. Growth, chlorophyll fluorescence, and camptothecin concentrations were determined. Flooding led to significant decreases in leaf biomass, while shading significantly increased stem biomass. Under full sunlight or slight shade conditions, lateral root biomass was significantly decreased by flooding, whereas under heavy shade, it was not significantly reduced by flooding. Shaded leaves displayed significantly higher chlorophyll fluorescence than the non-shaded leaves throughout the 6-week period. Onset of flooding caused immediate decline of chlorophyll fluorescence of nonshaded and slight-shaded seedlings, but the effect of flooding quickly diminished. Heavy shade led to elevated camptothecin concentrations in leaves, whereas flooding had no effect. Camptothecin concentrations in stems were not altered by either flooding or shading; however, they were substantially lowered in lateral roots under heavy shade compared with those grown in full sunlight. Flooding induced an additional camptothecin accumulation in lateral roots under slight shade, but it failed to do so under either heavy shade or full sunlight conditions. Key words: anti-tumor compounds, camptothecin, chlorophyll fluorescence, flooding, secondary metabolites, shading.

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Schweizer, Thorsten, Heiko Kubach, and Thomas Koch. "Investigations to characterize the interactions of light radiation, engine operating media and fluorescence tracers for the use of qualitative light-induced fluorescence in engine systems." Automotive and Engine Technology 6, no. 3-4 (October 23, 2021): 275–87. http://dx.doi.org/10.1007/s41104-021-00092-3.

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AbstractThe light-induced fluorescence (LIF) represents an important tool for the continuous improvement and further development of combustion engine systems regarding efficiency increase. In this work, the interactions between light-emitting diode (LED) and laser diode light radiation, engine oil/fuel and three fluorescence tracers are investigated on an application-related investigation system for the design of qualitative LIF experiments. Thereby two efficient light sources for engine combustion chamber lighting are presented. For different engine oil/fuel tracer combinations, the fluorescence is examined in its intensity and its spectrum depending on the temperature, concentration and temperature history. With oil temperature variations of up to 150 °C, changes in the fluorescence spectrum and fluorescence intensities that decrease by up to 80% are evident. For specific tracer oil/fuel mixtures, concentration-dependent maximum intensities and different temperature history behaviors can be revealed. The results shown support the design of spectral engine examination setups and give tracer dosage recommendations. Additionally, an engine LIF setup for in-cylinder tracking of engine operating media is presented.

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Chan, Cho X. J., and Peter N. Lipke. "Role of Force-Sensitive Amyloid-Like Interactions in Fungal Catch Bonding and Biofilms." Eukaryotic Cell 13, no. 9 (March 28, 2014): 1136–42. http://dx.doi.org/10.1128/ec.00068-14.

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ABSTRACTTheCandida albicansAls adhesin Als5p has an amyloid-forming sequence that is required for aggregation and formation of model biofilms on polystyrene. Because amyloid formation can be triggered by force, we investigated whether laminar flow could activate amyloid formation and increase binding to surfaces. ShearingSaccharomyces cerevisiaecells expressing Als5p orC. albicansat 0.8 dyne/cm2increased the quantity and strength of cell-to-surface and cell-to-cell binding compared to that at 0.02 dyne/cm2. Thioflavin T fluorescence showed that the laminar flow also induced adhesin aggregation into surface amyloid nanodomains in Als5p-expressing cells. Inhibitory concentrations of the amyloid dyes thioflavin S and Congo red or a sequence-specific anti-amyloid peptide decreased binding and biofilm formation under flow. Shear-induced binding also led to formation of robust biofilms. There was less shear-activated increase in adhesion, thioflavin fluorescence, and biofilm formation in cells expressing the amyloid-impaired V326N-substituted Als5p. Similarly,S. cerevisiaecells expressing Flo1p or Flo11p flocculins also showed shear-dependent binding, amyloid formation, biofilm formation, and inhibition by anti-amyloid compounds. Together, these results show that laminar flow activated amyloid formation and led to enhanced adhesion of yeast cells to surfaces and to biofilm formation.

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Cho, Hyejoung, Hui Zheng, Qiaochu Sun, Shuhan Shi, YuZhu He, Kyuhyeon Ahn, Byunggook Kim, Hye-Eun Kim, and Okjoon Kim. "Development of Novel Photosensitizer Using the Buddleja officinalis Extract for Head and Neck Cancer." Evidence-Based Complementary and Alternative Medicine 2018 (June 20, 2018): 1–10. http://dx.doi.org/10.1155/2018/6917590.

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Photodynamic therapy (PDT) is generally safer and less invasive than conventional strategies for head and neck cancer treatment. However, currently available photosensitizers have low selectivity for tumor cells, and the burden and side effects are so great that research is needed to develop safe photosensitizers. In this study, it was confirmed that the Buddleja officinalis (BO) extract, used in the treatment of inflammation and vascular diseases, shows fluorescence when activated by LED light, and, based on this, we aimed to develop a new photosensitive agent suitable for PDT. MTT, Diff-Quick® staining, and DCF-DA were performed to measure the effects of treating head and neck cancer cells with BO extract and 625 nm LED light (BO-PDT). Cell cycle, TUNEL, and western blot assays, as well as acridine orange staining, were performed to explore the mechanism of BO-PDT-induced cell death. We found that when the BO extract was irradiated with 625 nm LED light, it showed sufficient fluorescence and stronger intracellular toxicity and ROS effect than the currently commercially available hematoporphyrin. BO-PDT resulted in a decrease of mTOR activity that was correlated with an increase in the levels of ATG5, beclin-1, and LC3-II, which interfere with the formation of autophagosomes. In addition, BO-PDT induced the activation of PARP and led to an increase in the expression of proapoptotic protein Bax and a decrease in the expression of the antiapoptotic protein Bcl-2. Moreover, BO-PDT has been shown to induce the autophagy pathway 4 h after treatment, while apoptosis was induced 16 h after treatment. Finally, we confirmed that BO-PDT caused cell death of head and neck cancer cells via the intrinsic pathway. Therefore, we suggest that BO extract can be used as a new photosensitizer in PDT of head and neck cancer.

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Sato, Kazuyuki, Akira Kawasaki, Yukiko Karuo, Atsushi Tarui, Kentaro Kawai, and Masaaki Omote. "Synthesis of new fluorescent molecules having an aggregation-induced emission property derived from 4-fluoroisoxazoles." Beilstein Journal of Organic Chemistry 16 (June 22, 2020): 1411–17. http://dx.doi.org/10.3762/bjoc.16.117.

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Fluorescent molecules based on a fluorinated isoxazole scaffold were synthesized and investigated for their photochemical properties. The introduction of a fluorine substituent into 3,5-diarylisoxazoles led to an increase of fluorescence intensity and exhibited a redshift in the emission intensity. α-Fluorinated boron ketoiminates (F-BKIs) were also synthesized via a ring-opening reaction of 4-fluoroisoxazoles and exhibited highly fluorescent luminescence and aggregation-induced emission (AIE), showing promise as a new fluorophore.

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Lima, Elessandro Váguino de, Cristina Pacheco-Soares, and Newton Soares da Silva. "Photobiomodulation assay of muscle cells C2C12 after irradiation with LED device." Research, Society and Development 11, no. 6 (May 2, 2022): e41511628884. http://dx.doi.org/10.33448/rsd-v11i6.28884.

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Introduction: One of the ways that have been observed to reduce musculoskeletal fatigue is the use of protocols for the application of light sources (photobiomodulation) such as low-intensity laser and LED (Light Emitting Diode). Work involving photobiomodulation has shown promising results in strength performance or reduction of muscle fatigue. At the cellular level, photobiomodulation can modulate fibroblasts proliferation, the fixation and synthesis of collagen and procollagen, promote angiogenesis and improving energy metabolism in mitochondria. Compared with laser devices, LED has several advantages, such as being smaller, lighter, lower cost, and easier for operation. Objective: The present work objective is to verify if irradiation with LED device (650 nm and 860 nm) in muscle cells C2C12 modify the viability, morphology and cytoskeleton components. Methodology: C2C12 cells line (ATCC CRL - 1772) were cultured in 25 cm2 bottles at 37ºC under 5% CO2 in DMEM. The cells were irradiated with the light-emitting diodes (LED) device, Sportllux Ultra that consists of 84 LEDs, each individual LED has 8 mW of power, emitting in 660±20 nm (42 LEDs) and 850±20 nm (42 LEDs), and covering an area (A) of 120 cm2. The power density of delivered light was 5,6 mW/cm2, and the exposure time was 10 minutes, totalizing the fluence of 3,4 J/cm2. Viability assay was performed where the cells were incubated with 100 µL of Crystal Violet (CV) solution and mitochondrial activity assay was evaluated by the colorimetric MTT assay. Nucleus (DAPI) and Cytoskeleton (Rhodamine Phalloidin) fluorescence assay was performed to study the cytoskeleton based on the change in the actin filaments. Results: Our results demonstrate that the synergism of LED irradiation (660nm and 850nm) induced the proliferation of C2C12 cells. The light-emitting diode (LED) device, Sportllux Ultra has a significant effect on C2C12 cell culture. Mitochondrial activity and cell viability showed a significative increase in their activities after irradiation. The microscopy fluorescence observations showed an alignment of cytoskeletal components of C2C12 cells after irradiation. Conclusion: The application of irradiation with the Sportlux Ultra LED device stimulated an increase of energy by mitochondrial activity assay, number of cells by cell viability assay and alignment of cytoskeleton components by fluorescence assay in C2C12 line cells. Our results suggest that organizated cytoskeletal actin filaments normally contribute to cell survival and that induced major cell changes in the cytoskeleton that result in cell shape change. These results suggest that the Sport Lux Ultra LED device can help in the repair of tissue injuries and to collaborate to increase of performance in athletes in a faster way.

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Wei, Kaihua, Bojian Chen, Zejian Li, Dongmei Chen, Guangyu Liu, Hongze Lin, and Baihua Zhang. "Classification of Tea Leaves Based on Fluorescence Imaging and Convolutional Neural Networks." Sensors 22, no. 20 (October 13, 2022): 7764. http://dx.doi.org/10.3390/s22207764.

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The development of the smartphone and computer vision technique provides customers with a convenient approach to identify tea species, as well as qualities. However, the prediction model may not behave robustly due to changes in illumination conditions. Fluorescence imaging can induce the fluorescence signal from typical components, and thus may improve the prediction accuracy. In this paper, a tea classification method based on fluorescence imaging and convolutional neural networks (CNN) is proposed. Ultra-violet (UV) LEDs with a central wavelength of 370 nm were utilized to induce the fluorescence of tea samples so that the fluorescence images could be captured. Five kinds of tea were included and pre-processed. Two CNN-based classification models, e.g., the VGG16 and ResNet-34, were utilized for model training. Images captured under the conventional fluorescent lamp were also tested for comparison. The results show that the accuracy of the classification model based on fluorescence images is better than those based on the white-light illumination images, and the performance of the VGG16 model is better than the ResNet-34 model in our case. The classification accuracy of fluorescence images reached 97.5%, which proves that the LED-induced fluorescence imaging technique is promising to use in our daily life.

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Gałęcki, Krystian, Agnieszka Kowalska-Baron, Katarzyna E. Nowak, Anna Gajda, and Beata Kolesińska. "Steady-State and Time-Resolved Fluorescence Study of Selected Tryptophan-Containing Peptides in an AOT Reverse Micelle Environment." International Journal of Molecular Sciences 24, no. 20 (October 22, 2023): 15438. http://dx.doi.org/10.3390/ijms242015438.

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The aim of this study was to demonstrate the utility of time-resolved fluorescence spectroscopy in the detection of subtle changes in the local microenvironment of a tryptophan chromophore in a confined and crowded medium of AOT reverse micelles, which mimic biological membranes and cell compartmentalization. For this purpose, fluorescence properties of L-tryptophan and several newly synthesized tryptophan-containing peptides in buffer and in an AOT reverse micelle medium were determined. It was shown that insertion of tryptophan and its short di- and tripeptides inside micelles led to evident changes in both the steady-state emission spectra and in fluorescence decay kinetics. The observed differences in spectral characteristics, such as a blue shift in the emission maxima, changes in the average fluorescence lifetime, and the appearance of environmental-dependent fluorescent species, showed the utility of time-resolved fluorescence spectroscopy as a sensitive tool for detecting subtle conformational modifications in tryptophan and its peptides induced by changes in polarity, viscosity, and specific interactions between chromophores and water molecules/polar groups/ions that occur inside reverse micelles.

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da Silva, Airon José, Clístenes Williams Araújo do Nascimento, Artur da Silva Gouveia-Neto, and Elias Arcanjo da Silva-Jr. "LED-Induced Chlorophyll Fluorescence Spectral Analysis for the Early Detection and Monitoring of Cadmium Toxicity in Maize Plants." Water, Air, & Soil Pollution 223, no. 6 (March 23, 2012): 3527–33. http://dx.doi.org/10.1007/s11270-012-1130-8.

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Liu, Junfei, Ying Chen, Weiliang Duan, Yu Si, Ting Chen, and Zhiyang Dong. "Quantification of brown tide algae using EGAF coding and DFF feature fusion based on LED-induced fluorescence spectroscopy." Microchemical Journal 200 (May 2024): 110441. http://dx.doi.org/10.1016/j.microc.2024.110441.

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