Relevant bibliographies by topics / Lawsonia intracellularis

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
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Academic literature on the topic 'Lawsonia intracellularis'

Author: Grafiati
Published: 4 June 2021
Last updated: 20 February 2023

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Journal articles on the topic "Lawsonia intracellularis"

1

Nathues, H., R. Tegeler, and E. große Beilage. "Diagnostik in einem Mastschweinebestand mit Diarrhö." Tierärztliche Praxis Ausgabe G: Großtiere / Nutztiere 35, no. 02 (2007): 133–36. http://dx.doi.org/10.1055/s-0038-1624014.

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Zusammenfassung: Gegenstand und Ziel: In einem Mastbestand trat in einer Gruppe von etwa 200 Läuferschweinen bei 15–20% der Tiere Diarrhö auf. In einer ersten Untersuchung von zwei Analtupfern auf darmpathogene Infektionserreger konnte die Ätiologie nicht geklärt werden. Material und Methoden: Zehn Kotproben wurden mittels einer Multiplex-PCR auf spezifische Genomfragmente von Lawsonia intracellularis, Brachyspira (B.) hyodysenteriae und B. pilosicoli untersucht. Der Gehalt an Salmonella spp. und hämolysierenden Escherichia coli wurde mittels bakteriologischer Standardverfahren geprüft. Ergebnisse: In vier von zehn Kotproben konnte Lawsonia intracellularis nachgewiesen werden. Andere Enteritiserreger wurden nicht detektiert. Schlussfolgerung: Die Diarrhö im Mastbestand konnte auf eine Infektion mit Lawsonia intracellularis zurückgeführt werden. Klinische Relevanz: Zum Nachweis einer Infektionskrankheit in Herden muss stets eine ausreichende Anzahl Tiere untersucht werden. Für den Nachweis von Lawsonia intracellularis mittels PCR eignen sich Kot- und Schleimhautproben; Analtupfer sind ungeeignet.
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McCluskey, Jackie, Joanne Hannigan, Jennifer D. Harris, Brendan Wren, and David G. E. Smith. "LsaA, an Antigen Involved in Cell Attachment and Invasion, Is Expressed by Lawsonia intracellularis during Infection In Vitro and In Vivo." Infection and Immunity 70, no. 6 (June 2002): 2899–907. http://dx.doi.org/10.1128/iai.70.6.2899-2907.2002.

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ABSTRACT Lawsonia intracellularis has been identified recently as the etiological agent of proliferative enteropathies, which are characterized by intestinal epithelial hyperplasia and associated moderate immune responses. This disease complex has been reported in a broad range of animals, prevalently in pigs, and L. intracellularis has been linked with ulcerative colitis in humans. L. intracellularis is an obligate intracellular bacterium, and the pathogenic mechanisms used to cause disease are unknown. Using in vitro-grown organisms as a source of genomic DNA, we identified a Lawsonia gene which encodes a surface antigen, LsaA (for Lawsonia surface antigen), associated with attachment to and entry into cells. The deduced amino acid sequence of this protein showed some similarity to members of a novel protein family identified in a number of other bacterial pathogens but for which roles are not fully defined. Transcription of this gene was detected by reverse transcription-PCR in L. intracellularis grown in vitro in IEC18 cells and in bacteria present in ileal tissue from infected animals. Immunohistochemistry with specific monoclonal antibody and immunoblotting with sera from infected animals demonstrated that LsaA protein is synthesized by L. intracellularis during infection. Expression of this gene during infection in vitro and in vivo suggests that this surface antigen is involved during infection, and phenotypic analysis indicated a role during L. intracellularis attachment to and entry into intestinal epithelial cells
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Visscher, Christian, Jasmin Mischok, Saara Sander, Jutta Verspohl, Eva-Ursula Peitzmeier, Isabel von dem Busche, and Josef Kamphues. "Spread of an Experimental Salmonella Derby Infection in Antibiotic-Treated or Lawsonia intracellularis Vaccinated Piglets." Animals 8, no. 11 (November 12, 2018): 206. http://dx.doi.org/10.3390/ani8110206.

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Lawsonia intracellularis infections are a common reason for antibiotic treatment in pig production. Experimental studies in animals naturally infected with Lawsonia intracellularis comparing the course of an experimental Salmonella infection in piglets previously treated with tylosin or vaccinated against Lawsonia intracellularis are scarce. A total of 72 seven-week-old Salmonella-free pigs were taken from a herd with a Lawsonia intracellularis history in piglet rearing. The pigs were divided into two groups with three replicates each. Animals had either been previously treated with tylosin (10 mg/kg body weight) for seven days (AB+VAC−) or had been vaccinated as suckling pigs by drenching (Enterisol®Ileitis; AB−VAC+). Two animals per replicate were primarily infected with Salmonella Derby (1.04 × 108 colony-forming units per animal). The detection of Salmonella in faeces (p < 0.0001, odds ratio: 3.8364) and in the ileocaecal lymph nodes (p = 0.0295, odds ratio: 3.5043) was significantly more frequent in AB+VAC− animals. Overall, the odds ratio for detecting Salmonella in any substrate or organ was significantly higher in the AB+VAC− group animals (p = 0.0004, odds ratio: 5.9091). Treatment with tylosin can significantly increase the spread of a Salmonella infection, which is not observed after early Lawsonia intracellularis vaccination.
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HUSNÍK, R., J. KLIMEŠ, K. TOMANOVÁ, J. SMOLA, R. HALOUZKA, F. TICHÝ, and J. BRÁZDIL. "Lawsonia intracellularis in a dog with inflammatory bowel disease." Veterinární Medicína 48, No. 5 (March 30, 2012): 141–45. http://dx.doi.org/10.17221/5761-vetmed.

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A two-year-old male German short-haired pointer was presented with a 1.5-year history of intermittent small-bowel diarrhoea. Inflammatory bowel disease (chronic lymphocytic-plasmacytic gastritis, enteritis and colitis) was diagnosed on the basis of histological examination of biopsies obtained on repeated endoscopy and by exclusion of other possible causes. Warthin-Starry silver staining of stomach mucosa revealed the presence of gastric spiral organisms. The evidence of L. intracellularis was provided by a positive nested polymerase chain reaction in one biopsy of duodenal mucosa and in one rectal smear. In 5 blood sera collected over a period of 8 months the IgG antibodies to L. intracellularis were found by an indirect fluorescent antibody test. Treatment with oral prednisone led only to a temporary improvement.
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Ermolenko, A. N., E. E. Aуshpur, I. U. Mushtuk, V. V. Gumeniuk, V. V. Ukhovskyi, and V. V. Hlebeniuk. "Lawsonia intracellularis seroprevalence among the pig farm livestock of Ukraine." Theoretical and Applied Veterinary Medicine 8, no. 4 (2020): 261–64. http://dx.doi.org/10.32819/2020.84038.

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To study the seroprevalence of Lawsonia intracellularis among the pig livestock of Ukraine. The research was conducted in the laboratories of the Institute of Veterinary Medicine of NAAS and in pig farms. Were studied 653 samples of pigs’ serum of different age groups from17 pig farms with breeding stock of 40 456 sows. To study seroprevalence was used a test system for enzyme-linked immunosorbent assay based on monoclonal antibodies bioScreen Ileitis Antibody Test ELISA Svanovir®Lawsonia intracellularis (Boehringer Ingelheim Svanova). Serological tests have provided evidence that the pathogen was circulating in the herd. According to the research results, 46.4% of seropositive animals were found. Seropositive pigs were found in all studied farms, namely – among sows – 74.0%, replacement gilts – 79.6%, growing pigs – 2.5% and fattening pigs – 59.8%. The high level of seroprevalence among replacement gilts and sows of the 1st farrowing indicates the circulation of the pathogen in the herd, as well as the manifestation of morbidity in the fattening group, where was the highest rate of positive serum samples. From the 17 examined farms, only one farm was found where no seropositive animals were detected. According to our observations, proliferative enteropathy in pigs of Ukrainian pig farms has signs of chronic course, especially it is common in pig farms that import replacement gilts from abroad, where antibiotics are prohibited or restricted in donor farms. The results of serological tests allowed us to reconfirm that the spread of the disease is increasing in Ukrainian pig farms. Compared to 2015, the percentage of seropositive animals increased by almost 10%. The typical serological profile for Lawsonia intracellularis which is characteristic of our studied farms allowed us to conclude for which age groups of pigs and for which technological period of raising swine it is necessary to develop and implement measures for treatment and disease prevention. To control the disease, it is highly recommended to conduct constant monitoring of the farm’s well-being against ileitis (send, in addition to faecal samples for PCR testing, also serum for ELISA testing).
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Duhamel, G. E., E. C. Klein, R. O. Elder, and C. J. Gebhart. "Subclinical Proliferative Enteropathy in Sentinel Rabbits Associated with Lawsonia intracellularis." Veterinary Pathology 35, no. 4 (July 1998): 300–303. http://dx.doi.org/10.1177/030098589803500410.

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Light microscopic and ultrastructural changes of naturally acquired proliferative enteropathy were observed in two of three young sentinel New Zealand White rabbits. The etiologic agent, Lawsonia intracellularis, was demonstrated in the tissues using morphologic, immunohistochemical, and molecular methods. Proliferative enteropathy was associated with infection of villous and crypt enterocytes by intracellular organisms genotypically and antigenically related to L. intracellularis of various other animal species.
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Page, Allen E., Nathan M. Slovis, and David W. Horohov. "Lawsonia intracellularis and Equine Proliferative Enteropathy." Veterinary Clinics of North America: Equine Practice 30, no. 3 (December 2014): 641–58. http://dx.doi.org/10.1016/j.cveq.2014.08.001.

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Silva, Rodrigo Otávio Silveira, Michelle de Paula Gabardo, Juliana Saes Vilaça de Oliveira, Francisco Carlos Faria Lobato, and Roberto Maurício Carvalho Guedes. "Detection of Lawsonia intracellularis fecal shedding in dogs in Minas Gerais, Brazil." Ciência Rural 45, no. 9 (June 30, 2015): 1619–21. http://dx.doi.org/10.1590/0103-8478cr20141533.

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<p><bold>Lawsonia intracellularis</bold>is an obligate intracellular bacterium that is responsible for proliferative enteropathy, an enteric disease endemic in swine and common in foals. However, few studies have investigated this disease in dogs, and there are no reports of dogs infected with<bold> L. intracellularis</bold>in Latin America. The aim of this study was to evaluate the fecal shedding of<bold> L. intracellularis</bold>in diarrheic and non-diarrheic dogs in Minas Gerais, Brazil. A total of 58 dogs, 18 apparently healthy and 40 diarrheic, were examined in this study. DNA extracted from feces was analyzed using a nested PCR reaction to detect<bold> L. intracellularis.</bold>Three out of 40 (7.5%) diarrheic samples, all from 3-month-old puppies, were positive for<bold> L. intracellularis</bold>. These results highlight the need for additional studies to examine the role of this pathogen as a possible cause of enteric disease in dogs</p>
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C Rodríguez-Buenfil, Jorge, Mario J Alvarez-Fleites, and Rafael M Gómez-Medina. "Identificación de Lawsonia intracellularis en 20 granjas porcinas del estado de Yucatán." REVISTA BIOMÉDICA 11, no. 4 (October 1, 2000): 271–75. http://dx.doi.org/10.32776/revbiomed.v11i4.244.

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Introducción. La Enteropatía Proliferativa Porcina es una enfermedad emergente a nivel mundial, causada por una bacteria intracelular recientemente denominada Lawsonia intracellularis. Esta bacteria afecta a todos los cerdos observandose una mayor frecuencia en la población de engorda. En Yucatán no existe información sobre la presencia y distribución de esta enfermedad. Objetivo. Detectar la presencia de Lawsonia intracellularis en cerdos de engorda de granjas porcícolas del estado de Yucatán. Material y Métodos. Se trabajó un total de 20 granjas porcícolas las cuales fueron seleccionadas por conveniencia. Diez unidades de muestreo fueron seleccionadas de cada granja buscando la representatividad y considerando una prevalencia del 25% un nivel de confianza de 95% y una población promedio de 2000 animales. Cada unidad fue conformada recolectando heces de 4 animales de entre 30 y 90 Kg. La técnica diagnóstica que se utilizó en el laboratorio fue la de reacción en cadena de la polimerasa. Resultados. De un total de 20 granjas estudiadas 8 resultaron positivas a la presencia de Lawsonia intracellularis en la población de engorda. Esto representa un 40% de granjas infectadas con prevalencias ≥ al 25%. Con respecto al total de unidades de muestreo 23 (13%) resultaron positivas y 174 (87%) resultaron negativas. Se pudo observar una mayor frecuencia de granjas positivas con poblaciones de engorda mayor a 7000 cerdos de engorda. Conclusión. Se puede concluir que la bacteria Lawsonia intracellularis está presente en granjas porcinas del estado de Yucatán con prevalencias ≥ 25%.
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Szczotka-Bochniarz, Anna, Katarzyna Podgórska, Agnieszka Nowak, and Zygmunt Pejsak. "In vitro cultivation and immunostaining of Lawsonia intracellularis strains." Bulletin of the Veterinary Institute in Pulawy 57, no. 3 (September 1, 2013): 319–22. http://dx.doi.org/10.2478/bvip-2013-0055.

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Abstract The aim of the study was to implement in vitro cultivation of L. intracellularis strains using ATCC 55783 and vaccine strains, and McCoy cells (ATCC CRL-1696). The infection was monitored by daily observations under phase contrast microscope. Indirect immunostaining using monoclonal antibody was also performed. Large number of S-shaped, moving bacteria were found in the cell medium in cultures infected with ATCC 55783 and vaccine strain. Immunostaining revealed a high number of multiple cell-associated or intracellular red stained bacteria in the infected cultures. This study describes for the first time in vitro cultivation of L. intracellularis in Poland, which creates further perspective for more advanced research on this bacterium.
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Dissertations / Theses on the topic "Lawsonia intracellularis"

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Gomes, Nogueira Mariana. "Immunity of lawsonia intracellularis vaccination in pigs." Thesis, The University of Sydney, 2013. http://hdl.handle.net/2123/10421.

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ABSTRACT – Mariana Gomes Nogueira Lawsonia intracellularis is the causative agent of proliferative enteropathy (PE). PE is an important disease of weaner and grower pigs causing degrees of diarrhoea and negative effects on feed intake and weight gain. In-feed antibiotics are routinely used to control PE disease outbreaks. However, increasing restrictions to antibiotic use is being implemented in various pig producing countries. Therefore, alternatives to improve resistance while promoting growth performance are ideal. To limit the establishment of infection and increase profitability of pig production, disease prevention needs both nutritional and immunological strategies and well as effective sanitary measures. Since 2006, in the Australian market, a live attenuated Lawsonia intracellularis vaccine has been used to reduce clinical signs and PE lesions and reduce L. intracellularis shedding in faeces. However, the systemic and local immunological responses to a standard vaccine dose are poorly characterised. In the absence of proof that vaccinated pigs are protected from PE, veterinarians are unwilling to remove antibiotic medication for fear of acute haemorrhagic PE in adult pigs. Additionally, feeding strategies as alternatives to antibiotics, such as beta-glucan have been proposed as a possible option, but no study has investigated the effect of phytase on immune responses in growing pigs. The research in this thesis addresses studies to immune-based investigation of factors affecting the induction of immune responses following vaccination with L. intracellularis. Results revealed that the use of an oral standard and ten times dose of L. intracellularis vaccine protects pigs against PE disease by reducing lesions, shedding in faeces and clinical signs. However, intramuscular delivery also protected pigs against PE. Immunological responses to L. intracellularis vaccination, particularly IgG and cytokines response were observed after oral, intraperitoneal and intramuscular L. intracellularis vaccination. However these were highly variable, highlighting the difficulties in finding suitable biomarkers. The effect of adding S. cerevisae yeast beta-glucan and microbial phytase to weaner diet affected on mucosal and systemic L. intra
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Vu, Ngoc Quynh. "Studies on Lawsonia intracellularis infection in pigs." Thesis, The University of Sydney, 2004. https://hdl.handle.net/2123/28098.

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The porcine proliferative enteropathies (PPE) include a variety of pathological conditions commonly described as separate entities: these include proliferative haemorrhagic enteropathy (PHE), necrotic enteritis (NE), regional ileitis (RI) and porcine intestinal adenomatosis (PIA). All of these pathological conditions are characterised by development of proliferation of the intestinal mucosa, particularly in the distal small intestine and proximal large intestine and the presence of curved, rod-shaped, intracellular bacteria, Lawsonia intracellularz‘s, in the apical cytoplasm of the epithelial cells. PPE can occur in all swine production systems and involve pigs of all age groups, however the more severe clinical from, PHE that causes higher mortality, predominantly affects adult pigs. In the endemic herds, pigs can become infected with L. intracellularis at different time points and shed bacteria in their faeces. Although factors that may play a role in the transmission of L. intracellularis infection have not been identified, spreading of the infection between pigs through the ingestion of contaminated faeces is considered the most important mode of transmission. Using an oral challenge model, this thesis examined how the number of ingested L. intracellularz's could affect the establishment, temporal pattern of infection, clinical disease and immune response to the infection in pigs. Susceptible pigs of all ages exposed to L. intracellularis develop infection and an immune response. They shed L. intracellularis in their faeces and, as the bacterium can not be grown in any artificial medium, the recent development of polymerase chain reaction (PCR) allows the detection of a current infection with L. intracellularis in live pigs by detecting L. intracellularis DNA in the contaminated faeces. Development of immune response to L. intracellularis in infected pigs by production of specific immunoglobulins including IgG, IgM, and IgA in the serum can be detected by the indirect immunofluorescent antibody test (IFAT). One of the aims of this thesis was to investigate the extent of the immunity to L. intracellularis in protecting previously exposed animals from re-infection. In—feed administration of antibiotics is widely practiced in pig production to control many bacterial infections including L. intracellularis as well as for growth promotion. It is important to assess the effect of different groups of antibiotics at different concentrations on the disease pattern and on the development of immunity to re-infection in pigs. Optimising the selection and concentration of some antibacterials can help to reduce the amount and extent of drug use while maintaining a good health status of a pig herd and improving profitability of pig production. As a part of this thesis, an investigation was conducted to determine if L. intracellularis existed in the system of commercial pig farms in the North of Vietnam. Some piggery management factors which may be associated with the occurrence of L. intracellularis infection in these pig herds were also identified and analyzed. The laboratory techniques developed in Australia such as faecal PCR and IFAT appeared to work well and to be useful as tools for diagnosis and survey of L. intracellularis under Vietnamese conditions.
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Nathues, Heiko. "Untersuchungen zur Ausscheidung von Lawsonia intracellularis mittels molekularbiologischer Untersuchungsverfahren." [S.l.] : [s.n.], 2007. http://deposit.ddb.de/cgi-bin/dokserv?idn=985342978.

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Collins, Alison Marie. "A Study of the Lawsonia intracellularis-induced porcine proliferative enteropathies." University of Sydney. Veterinary Science, 2001. http://hdl.handle.net/2123/835.

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The porcine proliferative enteropathies (PPE) are a group of diseases ranging from intestinal adenomatosis (PIA), a chronic condition causing reduced growth rates in post weaning pigs, to the often fatal proliferative haemorrhagic enteropathy (PHE), resulting in intestinal haemorrhage. PHE predominantly occurs in older and heavier pigs than the chronic disease PIA. This thesis examined whether the age when susceptible pigs are infected affects the clinical response to L.intracellularis infection. The characteristic pathologic lesion of PPE is the abnormal proliferation of crypt epithelial cells in the ileum and colon. Closely associated with this proliferation is the presence of an obligately intracellular bacterium, Lawsonia intracellularis. Characterisation of L.intracellularis was performed in in-vitro co-cultures of L.intracellularis extracted from PHE-affected mucosa. The efficacy of antimicrobials to inhibit the growth of L.intracellularis in-vitro was evaluated and compared with isolates cultured in the United Kingdom. The results were analysed with respect to medication strategies currently used to control PPE in piggeries. PPE occurs in virtually all piggery management systems, including newly developed systems that are aimed at improving the herd health, such as segregated early weaning and multiple site production. PPE is currently controlled in Australia with the routine addition of antimicrobials in pig feed, in particular olaquindox. Recommendations to reduce the use of feed-based antibiotics in Australia require the development of alternate strategies to control diseases such as PPE. Sequential outbreaks of PHE reported in minimal disease herds suggested that pigs could develop immunity to disease. An experimental model of L.intracellularis infection was developed in this thesis to demonstrate that immunity to re-infection with L.intracellularis could be developed. Infection was monitored by detection of faecal shedding of L.intracellularis and serum IgG antibodies against L.intracellularis. Two in-feed antimicrobial strategies were analysed in this thesis for their ability to induce the development of immunity to L.intracellularis, while avoiding clinical signs of disease. The first strategy evaluated the use of low levels of in-feed antimicrobials to allow subclinical infection and the development of immunity. The second strategy evaluated the use of high levels of in-feed antimicrobials to terminate infection two weeks after exposure to L.intracellularis. Gaining a greater understanding of how L.intracellularis infection is spread both within and between piggeries will enable the development of management strategies to control the spread of infection. This thesis examined the possibility that other species in contact with pigs and piggeries such as rats, mice and birds may transmit infection to pigs. The transmission of infection between pigs via the faecal/oral route was also examined, as was the survival and infectivity of L.intracellularis over time. Ultimately this thesis aimed to understand the pattern of L.intracellularis infection and the survival and transmission of L.intracellularis in order to develop effective control measures for PPE, especially in minimal disease herds.
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Collins, Alison Marie. "A Study of the Lawsonia intracellularis-induced porcine proliferative enteropathies." Thesis, The University of Sydney, 2000. http://hdl.handle.net/2123/835.

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The porcine proliferative enteropathies (PPE) are a group of diseases ranging from intestinal adenomatosis (PIA), a chronic condition causing reduced growth rates in post weaning pigs, to the often fatal proliferative haemorrhagic enteropathy (PHE), resulting in intestinal haemorrhage. PHE predominantly occurs in older and heavier pigs than the chronic disease PIA. This thesis examined whether the age when susceptible pigs are infected affects the clinical response to L.intracellularis infection. The characteristic pathologic lesion of PPE is the abnormal proliferation of crypt epithelial cells in the ileum and colon. Closely associated with this proliferation is the presence of an obligately intracellular bacterium, Lawsonia intracellularis. Characterisation of L.intracellularis was performed in in-vitro co-cultures of L.intracellularis extracted from PHE-affected mucosa. The efficacy of antimicrobials to inhibit the growth of L.intracellularis in-vitro was evaluated and compared with isolates cultured in the United Kingdom. The results were analysed with respect to medication strategies currently used to control PPE in piggeries. PPE occurs in virtually all piggery management systems, including newly developed systems that are aimed at improving the herd health, such as segregated early weaning and multiple site production. PPE is currently controlled in Australia with the routine addition of antimicrobials in pig feed, in particular olaquindox. Recommendations to reduce the use of feed-based antibiotics in Australia require the development of alternate strategies to control diseases such as PPE. Sequential outbreaks of PHE reported in minimal disease herds suggested that pigs could develop immunity to disease. An experimental model of L.intracellularis infection was developed in this thesis to demonstrate that immunity to re-infection with L.intracellularis could be developed. Infection was monitored by detection of faecal shedding of L.intracellularis and serum IgG antibodies against L.intracellularis. Two in-feed antimicrobial strategies were analysed in this thesis for their ability to induce the development of immunity to L.intracellularis, while avoiding clinical signs of disease. The first strategy evaluated the use of low levels of in-feed antimicrobials to allow subclinical infection and the development of immunity. The second strategy evaluated the use of high levels of in-feed antimicrobials to terminate infection two weeks after exposure to L.intracellularis. Gaining a greater understanding of how L.intracellularis infection is spread both within and between piggeries will enable the development of management strategies to control the spread of infection. This thesis examined the possibility that other species in contact with pigs and piggeries such as rats, mice and birds may transmit infection to pigs. The transmission of infection between pigs via the faecal/oral route was also examined, as was the survival and infectivity of L.intracellularis over time. Ultimately this thesis aimed to understand the pattern of L.intracellularis infection and the survival and transmission of L.intracellularis in order to develop effective control measures for PPE, especially in minimal disease herds.
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Mitchell, Suzanne Christina. "Immune responses in a mouse model of Lawsonia intracellularis infection." Thesis, University of Edinburgh, 2002. http://hdl.handle.net/1842/29892.

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The aim of this thesis was to establish an infection system in the mouse and use this to examine the development of infection and lesions of proliferative enteropathy within the gastrointestinal tract. This mouse model was then used to assess the mucosal and serological responses generated by the host in response to inoculation with L. intracellularis. The infection system demonstrated that 129/Sv/Ev wild-type and isogenic interferon gamma receptor null (IFNγR-/-) mice developed characteristic lesions of proliferative enteropathy following oral challenge with a pure culture of L. intracellularis (approximately 5xl07 organisms). These were directly comparable to the lesions seen in natural disease with increased proliferation of infected enterocytes in the ileum and colon associated with the loss of goblet cells. Wild-type 129/Sv/Ev mice appeared capable of resolving or resisting infection with this obligate intracellular pathogen, with lesions of proliferative enteropathy detectable only at day 14 or 21 post inoculation, thereafter (days 28 and 35) there were none detectable. In comparison, IFNγR-/- mice were highly susceptible to disease and appeared unable to eliminate infection over the time course studied, indicating that a functional IFNγ receptor is necessary in the control of infection. This novel system is the first detailed description of proliferative enteropathy in mice and has demonstrated the usefulness of this model in the characterisation and analysis of important immunological events during infection with Lawsonia intracellularis.
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Collins, Alison Marie. "A study of the Lawsonia intracellularis-induced porcine proliferative enteropathies." Connect to full text, 2000. http://hdl.handle.net/2123/835.

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Thesis (Ph. D.)--University of Sydney, 2001.
Includes tables. Title from title screen (viewed Apr. 22, 2008). Submitted in fulfilment of the requirements for the degree of Doctor of Philosophy to the Dept. of Veterinary Clinical Sciences, Faculty of Veterinary Science. Degree awarded 2001; thesis submitted 2000. Includes bibliography. Also available in print form.
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McAllister, G. E. "Molecular and functional characterisation of the immunodominant antigens of the obligate intracellular pathogen Lawsonia intracellularis." Thesis, University of Edinburgh, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.657505.

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TlyA deletion mutants in H. pylori and B. hyodysenteriae are attenuated, suggesting that these proteins perform important roles during infection. LsaA (lawsonia surface antigen) the L. intracellularis orthologue, is expressed during infection in vitro and in vivo and suggest that this factor is involved during adherence and/or invasion of intestinal epithelial cells. The principal aim was to characterise function(s) of LsaA. Specifically the putative function as an adhesin was investigated further using a combination of biochemical and molecular approaches (including affinity purification and yeast 2-bybrid) to elucidate possible receptor(s). However, no consistent partner was evident therefore mammalian epithelial cell receptors could not be defined using this range of approaches. It is possible that LsaA’s role in adherence is adventitious – for example, it has been proposed that the TlyA family of proteins possess a regulatory role in bacterial colonisation as opposed to a direct involvement in bacterial adherence. The existence of two conserved putative functional domains S4RNA binding and methyltransferase motifs have been noted in all members of the TlyA family examined to date. These domains are found separately in several protein families known to be involved in gene regulation. Since no system has been developed for mutating genes in L. intracellularis the proteome of a TlyA deletion mutant of H. pylori was compared to its parent to further this potentially new and interesting function of TlyA family proteins. Notably, flagellin B and catalase were absent in the tlyA mutant. Since deletion of tlyA corresponds with changes in expression of several H. pylori genes, it can be concluded that reduced colonisation of H. pylori tlyA mutant is likely to be as a result of effects on expression of virulence genes rather than a direct role in adherence.
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Wenting, Sabine [Verfasser]. "Untersuchungen zur Prävalenz der Lawsonia intracellularis-Infektion bei Absetzferkeln / Sabine Wenting." Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2013. http://d-nb.info/1037887735/34.

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Gomes, Cleise Ribeiro. "Detecção de Lawsonia intracellularis em aves comerciais através da reação em cadeia pela polimerase (PCR)." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-25052007-130622/.

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A Lawsonia intracellularis é uma bactéria intracelular obrigatória que causa Enterite Proliferativa em vários animais, como suínos, cervos, ratos, hamsters, cobaios, coelhos, ovinos, eqüinos, raposas, cães, furões, primatas não humanos, emas e avestruzes. Atualmente não há relatos da ocorrência do agente ou dos sinais clínicos de sua infecção em aves comerciais (Gallus gallus domesticus). O presente estudo reporta a detecção através da reação em cadeia pela polimerase (PCR) de Lawsonia intracellularis em matrizes pesadas de diferentes idades provenientes de quatro linhagens de aves comerciais. Dentre os 100 suabes de fezes colhidos a partir de fragmentos intestinais, 34% foram positivos para a detecção do agente pela PCR. O agente foi encontrado com maior freqüência em aves de 80 semanas de idade apresentando ou não quadro diarréico. Os fragmentos intestinais das aves cujas fezes foram positivas para detecção de Lawsonia intracellularis foram submetidos ao exame histopatológico e na maioria das lesões analisadas foi observada enterite necrótica crônica proliferativa. Apesar das lesões serem sugestivas de infecção por Lawsonia intracellularis pela coloração de Warthin-Starry, a presença do patógeno no interior dos enterócitos ou células glandulares não foi observada. Esses resultados poderiam levar a novas pesquisas sobre a importância da Lawsonia intacellularis na avicultura do Brasil e do mundo.
Lawsonia intracellularis is an obligate intracellular bacterium responsible for proliferative enteritis in many animals, such as swine, deers, rats, hamsters, guinea pigs, rabbits, ovine, horses, foxes, dogs, ferrets, no human primats, emus and ostrichs. Currently there is no reports about the occurrence of this agent or clinical signs of its infection in chickens (Gallus gallus domesticus). The present study reports the detection of Lawsonia intracellularis at different ages of broiler breeder in four avian commercials lineages by Polymerase Chain Reaction (PCR). Of 100 faecal swabs collected from intestinal fragments, 34% were positive for Lawsonia intracellularis detection by PCR. The agent was more frequently found in chickens of 80 weeks of age presenting or not diarrhea. The intestinal fragments which faeces were positive for Lawsonia intracellularis PCR detection were submitted to histopathological exam and in the most analysed lesions were observed proliferative necrotic cronic enteritis. Although the lesions were suggestive of L. intracellularis infection by Warthin-Starry staining, the presence of the pathogen inside of enterocytes or gland cells was not observed. This results could lead to new researches about the mean of Lawsonia intacellularis in broiler industry of Brazil and the world.
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Books on the topic "Lawsonia intracellularis"

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Lawsonia intracellularis. Servet, 2019.

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Book chapters on the topic "Lawsonia intracellularis"

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Gebhart, C. J., and R. M. C. Guedes. "Lawsonia Intracellularis." In Pathogenesis of Bacterial Infections in Animals, 503–12. Oxford, UK: Wiley-Blackwell, 2010. http://dx.doi.org/10.1002/9780470958209.ch26.

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Gebhart, Connie J. "Lawsonia intracellularis." In Interpretation of Equine Laboratory Diagnostics, 185–89. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2017. http://dx.doi.org/10.1002/9781118922798.ch33.

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Pusterla, Nicola, Connie J. Gebhart, Jean-Pierre Lavoie, and Richard Drolet. "Lawsonia intracellularis." In Equine Infectious Diseases, 316–21. Elsevier, 2014. http://dx.doi.org/10.1016/b978-1-4557-0891-8.00034-8.

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Lavoie, Jean-Pierre, and Richard Drolet. "Lawsonia intracellularis." In Equine Infectious Diseases, 313–16. Elsevier, 2007. http://dx.doi.org/10.1016/b978-1-4160-2406-4.50041-7.

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LAVOIE, J. "Lawsonia intracellularis Proliferative Enteropathy." In Current Therapy in Equine Medicine, 164–66. Elsevier, 2003. http://dx.doi.org/10.1016/b978-0-7216-9540-2.50051-7.

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Frazer, Michele L. "Lawsonia intracellularis Infection and Proliferative Enteropathy." In Robinson's Current Therapy in Equine Medicine, 345–48. Elsevier, 2015. http://dx.doi.org/10.1016/b978-1-4557-4555-5.00079-0.

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Conference papers on the topic "Lawsonia intracellularis"

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Tomanova, Katarina, and Vladimir Celer. "Selection of recombinant antibodies against Lawsonia intracellularis." In Sixth International Symposium on the Epidemiology and Control of Foodborne Pathogens in Pork. Iowa State University, Digital Press, 2005. http://dx.doi.org/10.31274/safepork-180809-763.

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Nerem, J. "Lawsonia intracellularis vaccination as an alternative to dietary antimicrobial medication in finishing swine." In Eighth International Symposium on the Epidemiology and Control of Foodborne Pathogens in Pork. Iowa State University, Digital Press, 2009. http://dx.doi.org/10.31274/safepork-180809-836.

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Zhang, Min, Huifang Yin, Ailing Dai, Xiao hua, and Xiaoyan Yang. "Prokaryotic Expression and Purification of LscW Protein of Lawsonia intracellularis for biomedical engineering." In 2019 IEEE International Conference on Computation, Communication and Engineering (ICCCE). IEEE, 2019. http://dx.doi.org/10.1109/iccce48422.2019.9010773.

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Larsen, I., A. Mellerup, C. Zachariasen, S. Saxmose Nielsen, A. Folkesson, Ø. Angen, L. Christiansen, et al. "Effect of variation in oxytetracycline treatment of Lawsonia intracellularis diahrea in nursery pigs on treatment-efficacy and resistance development." In Fourth International Symposium on the Epidemiology and Control of Salmonella and Other Food Borne Pathogens in Pork. Iowa State University, Digital Press, 2015. http://dx.doi.org/10.31274/safepork-180809-262.

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Artuso Ponte, Valeria, Melanie Abley, Bayleygn Z. Molla, Gireesh Rajashekara, Prosper Boyaka, and Wondwossen A. Gebreyes. "Activity of Sangrovit® against Lawsonia intracellularis in grower pigs and its impact on gut physiology and host immunity." In Ninth International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork. Iowa State University, Digital Press, 2011. http://dx.doi.org/10.31274/safepork-180809-668.

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Leite, Fernando, Connie Gebhart, Randall Singer, and Richard Isaacson. "Vaccination against Lawsonia intracellularis decreases shedding of Salmonella enterica serovar typhimurium in co-infected pigs and changes the host gut microbiome." In Safe Pork 2015: Epidemiology and control of hazards in pork production chain. Iowa State University, Digital Press, 2017. http://dx.doi.org/10.31274/safepork-180809-367.

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Reports on the topic "Lawsonia intracellularis"

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Magtoto, Ronaldo L., John K. Johnson, and Sheela Ramamoorthy. Lawsonia intracellularis ELISA: A New Test at the ISU-VDL. Ames (Iowa): Iowa State University, January 2010. http://dx.doi.org/10.31274/ans_air-180814-645.

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