Dissertations / Theses on the topic 'Lates Calcarifer'
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Griffiths, Neil R. "Gill disease in barramundi (Lates calcarifer)." Thesis, Griffiths, Neil R. (2009) Gill disease in barramundi (Lates calcarifer). Masters by Research thesis, Murdoch University, 2009. https://researchrepository.murdoch.edu.au/id/eprint/2434/.
Full textGibson-Kueh, Susan. "Diseases of Asian seabass (or barramundi), Lates calcarifer Bloch." Thesis, Gibson-Kueh, Susan (2012) Diseases of Asian seabass (or barramundi), Lates calcarifer Bloch. PhD thesis, Murdoch University, 2012. https://researchrepository.murdoch.edu.au/id/eprint/14817/.
Full textMarshall, Carina Rynn Ecremen. "Evolutionary genetics of barramundi (Lates calcarifer) in the Australian region." Thesis, Marshall, Carina Rynn Ecremen (2005) Evolutionary genetics of barramundi (Lates calcarifer) in the Australian region. PhD thesis, Murdoch University, 2005. https://researchrepository.murdoch.edu.au/id/eprint/181/.
Full textMarshall, Carina Rynn Ecremen. "Evolutionary genetics of barramundi (Lates calcarifer) in the Australian region." Marshall, Carina Rynn Ecremen (2005) Evolutionary genetics of barramundi (Lates calcarifer) in the Australian region. PhD thesis, Murdoch University, 2005. http://researchrepository.murdoch.edu.au/181/.
Full textcom, cmarshall@tobob, and Carina Rynn Ecremen Marshall. "Evolutionary Genetics of Barramundi (Lates Calcarifer)in the Australian Region." Murdoch University, 2005. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20050421.134447.
Full textBromage, Erin. "The humoral immune response of Lates calcarifer to Streptococcus iniae." Thesis, Townsville, Qld, 2004. https://researchonline.jcu.edu.au/1007/1/01front.pdf.
Full textBromage, Erin. "The humoral immune response of Lates calcarifer to Streptococcus iniae." Townsville, Qld, 2004. http://eprints.jcu.edu.au/1007/1/01front.pdf.
Full textSiddik, Muhammad Abu Bakar. "Physiological Responses of Juvenile Barramundi (Lates calcarifer) Fed Processed Animal Protein Diets." Thesis, Curtin University, 2018. http://hdl.handle.net/20.500.11937/75651.
Full textYounus, Zakhariya Sona. "Effects of pre and post freezing treatments on barramundi (Lates calcarifer, Bloch) fillet quality." Thesis, Curtin University, 2014. http://hdl.handle.net/20.500.11937/1653.
Full textRussell, David John. "Some aspects of the biology of the Barramundi, Lates calcarifer (Bloch) in Eastern Queensland." Thesis, Queensland University of Technology, 1990. https://eprints.qut.edu.au/35966/1/35966_Russell_1990.pdf.
Full textVo, Binh Van. "Physiological responses of juvenile barramundi, Lates calcarifer (Bloch, 1790) when fed bioprocessed plant base diets." Thesis, Curtin University, 2017. http://hdl.handle.net/20.500.11937/59626.
Full textKinhult, Anne. "Barramundi (Lates calcarifer) IGF-I : characterization of cDNA, genomic sequences, and regulation of mRNA expression." Thesis, Queensland University of Technology, 1996.
Find full textEtzion, Asaf. "The Effect of probiotics on the innate immune system and disease resistance of Barramundi, Lates calcarifer /." Sde Boker [Israel] : Ben-Gurion University of the Negev, 2008. http://aranne5.lib.ad.bgu.ac.il/others/EtzionAsaf.pdf.
Full textChiew, Ivan Kar Mun. "Resistant starch from underutilised legumes as prebiotic and its effect on the growth of Danio rerio and Lates calcarifer." Thesis, University of Nottingham, 2018. http://eprints.nottingham.ac.uk/51752/.
Full textChaklader, Md Reaz. "Supplementing insect meal and fish protein hydrolysates in barramundi, Lates calcarifer diet improves the inclusion efficiency of poultry by-product meal: a physiological approach." Thesis, Curtin University, 2021. http://hdl.handle.net/20.500.11937/86668.
Full textGuiguen, Yann. "Approches morphologique, histologique et endocrinienne des cycles reproducteurs et de l'inversion sexuelle chez un poisson hermaphrodite protandre : le loup tropical, lates calcarifer, introduit en elevage en polynesie francaise." Rennes 1, 1992. http://www.theses.fr/1992REN10133.
Full textDegger, Brian. "Fish insulin-like growth factors : their role in growth from a functional perspective." Thesis, Queensland University of Technology, 2001.
Find full textRoe, Brett, and b. roe@cqu edu au. "Ecologically Engineered Primary Production in Central Queensland, Australia - Integrated Fish and Crayfish Culture, Constructed Wetlands, Floral Hydroponics, and Industrial Wastewater." Central Queensland University. Sciences, 2005. http://library-resources.cqu.edu.au./thesis/adt-QCQU/public/adt-QCQU20080717.092551.
Full textTruong, Binh. "High pressure processing of Barramundi fish (Lates calcarifer)." Thesis, 2017. http://hdl.handle.net/1959.13/1354648.
Full textHigh pressure processing (HPP) is well-known as an innovative processing technology that can extend shelf-life and improve physicochemical properties of fish muscle during storage. However, research for all potential HPP applications on barramundi fish has not been done before. In this study, HPP was applied prior to conventional freezing to improve physicochemical properties of barramundi muscle and to abate the adverse effects of freezing on barramundi muscle during frozen storage. HPP was also used with or without the combination of chitosan coating to extend the shelf-life of barramundi during chilled storage. HPP was also employed to produce barramundi gels, especially, to reduce salt concentration in these gels. For raw barramundi muscle stored in frozen condition at - 18 °C, application of HPP at 150 MPa and initial temperature of 4 °C for 3 min prior to freezing resulted in barramundi fillet with higher hardness and springiness, lower drip loss and stable pH compared to non-pressurised samples during frozen storage for up to 18 weeks. HPP under these conditions also did not induce cooked appearance and lipid oxidation of barramundi fillet, two major drawbacks in pressurised fish muscle. Thus, HPP prior to freezing may be a good option for barramundi processors. Before chilled storage at 4 ± 1 °C, raw barramundi muscle was treated under 3 different conditions including pressurisation (HPP), chitosan coating and pressurisation (HC) and chitosan coating (CHI). Barramundi samples were investigated on day 1, 8, 15 and 23 during chilled storage. HPP at 200 MPa and 4 ± 1 °C for 3 min significantly improved texture profile, pH, drip loss and TVB-N of barramundi muscle as compared to CHI and control treatment. However, HPP did not delay microbial growth compared to CHI and control treatment. Application of HC treatment significantly hampered the development of TVB-N, microbial growth and improved texture and drip loss of barramundi muscle compared to control, HPP and CHI treatment. However, HC treatment resulted in a cooked appearance and acceleration of lipid oxidation, two major drawbacks in pressurised fish muscle. Pressurisation significantly decreased the activity of protease, but did not reduce the activity of LOX. Microscopic images also showed that the microstructures of pressurised samples were better preserved than the cracked microstructure of unpressurised samples. In this study, HPP was found to be the most favourable treatment for improving the quality of barramundi muscles stored in chilled condition for up to 23 days. For the application of HPP to produce barramundi gels, barramundi minced muscles with 1% and 2% added salt were pressurised at 300, 400 and 500 MPa at ≤ 10 °C and 50 °C for 10 min. Pressure induced barramundi gels (PG) exhibited a lower gel strength and poorer texture such as hardness and springiness as compared to conventional heat induced gels. Comparable water holding capacity to heat induced gels was only obtained at a salt concentration of 2% and at pressures ≥ 400 MPa. SEM images showed a compact network with smoother surface of barramundi minced muscle with 2% added salt and HPP at ≥ 400 MPa as compared to conventional heat induced gels. Gelling properties of barramundi minced muscle with 1.5% and 2 % added salt were assessed after HPP at 300, 400 and 500 MPa at 4 °C (initial temperature) for 10 min and subsequent cooking at 90 °C for 30 min. Whiteness, gel forming ability, water holding capacity, hardness and springiness of the barramundi gels increased as applied pressure and salt concentration increased. At 2% salt concentration, HPP resulted in barramundi gels with higher gel strength and smoother texture as compared to conventional heat induced gels (0.1 MPa, 90 °C for 30). At a reduced salt concentration (1.5%) and HPP at ≥ 400 MPa, the quality (gel strength, water holding capacity, hardness and springiness) of pressurised, cooked gels are comparable to those heat-induced gels with 2% added salt, but the microstructure is smoother. Scanning electron microscope images of pressurised, cooked gels showed a compact network with smoother surface than those of heat-only induced gels. Thus, application of HPP prior to cooking could be an effective method to enable reduced salt concentration in barramundi gels. Barramundi minced muscle with 1% and 2% added salt was gelled by different combinations of pressurisation (300, 400 and 500 MPa at 4 °C for 10 min), cooking (0.1 MPa, 90°C for 30 min) and setting (0.1 MPa, 50 °C for 2 h) to improve their mechanical properties and lower the amount of salt added to barramundi gels. At the low salt concentration of 1%, pressurisation prior to cooking (P - C) treatment resulted in barramundi gels with comparable mechanical properties and water holding capacity to those of conventional heat induced (HI) gels with 2% added salt. At a salt concentration of 2%, pressurisation prior to setting (P - S) and P - C gels exhibited higher mechanical properties and water holding capacity than HI gels. Scanning electron microscope images showed a smooth and dense microstructure of P - C and P - S gels, whereas the microstructure of HI gels is rough and less compact. P - S and P - C treatment can result in higher mechanical and functional properties of barramundi gels at conventional salt concentration (2%) compared to HI gels. P - C treatment can lower the salt concentration added to barramundi gels to 1% which is very significant for health-conscious consumers. To produce a shelf-stable barramundi product by high pressure thermal sterilisation (HPTS), barramundi muscle in a brine was treated at 600 MPa and three different temperatures (90, 110 and 120 °C) for 5 min. Barramundi muscle retorted at Fo = 3.38 was used as control. HPTS at 600 MPa and 110 °C and 120 °C, for 5 min, respectively, produced stable barramundi products, which were stored at room temperature and tested for up to 1 year. Hardness and springiness, of HPTS sterilised barramundi samples were enhanced (i.e. increased) compared to retorted samples. Gumminess, chewiness and cohesiveness of HPTS sterilised barramundi muscle were also similar to retorted samples and did not decrease as processing temperature increased. TBA and pH was also similar in all treatments, except for a significant increase of pH of samples treated at 600 MPa and 90 °C for 5 min after 3 months of storage. In general, HPTS could be a feasible option to produce a sterilised barramundi product with better overall quality and is recommended for more research on other HPTS barramundi products.
Tsai, Ching-Yi, and 蔡靜宜. "Application of fish vaccine on giant seaperch , Lates calcarifer." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/23130401593484456808.
Full text國立屏東科技大學
水產養殖系
92
In recent years, the infection disease caused by neuron necrosis virus (NNV) and grouper iridovirus (GIV) dozen of important aquaculture industries in the world. The species affected include fish, amphibian and reptile, and the habitat covered from temperate zone to tropical zone and from seawater to freshwater. To prevent and treat this virus infections are therefore top urgent issues right now. In this study, we prepared formalin inactivated vaccines for yellow grouper neuron necrosis virus (YGNNV) and grouper iridovirus (GIV). The result showed, after injection in combinations of various vaccines treatments injected GIV, YGNNV, and YGNNV+GIV vaccines, respectively the specific immunoreaction of treated seaperch’s tissue fluid were detected by the enzyme-linked immunosorbent assay (ELISA). Soaking in inoculated vaccine three times, the tested fry exhibited non-specific immune response. While soaking in same vaccine twice and injecting it one, the fry showed obvious specific immune response (p<0.05). After three months of inoculated vaccine, the fry injected with YGNNV+GIV treatment has the best survival rate during YGNNV and GIV virus challenge test. This fact implies that mixing vaccines (YGNNV+GIV) possess not only the specific immunoreaction but non-specific protective effects. For the accumulative mortality in field trials, we found that inoculated vaccine injection has a better protective and higher safety effects than soaking one.
Chang, Meng-Chia, and 張孟嘉. "Physiological stress responses in fasting Asian seabass (Lates calcarifer)." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/2zd33m.
Full text國立中山大學
海洋科學系研究所
107
Fasting is a common process in aquaculture industry. Fasting was thought to influence physiological performance and homeostasis of fishes. To clarify and evaluate physiological changes through peroids of time in fish subjected to fasting is crucial for aquacultural practice. In this study, the Asian seabass (Lates calcarifer) were fasted for 1, 4 and 7 days to evaluate the physiological responses of fish. The secondary stress responses were examined, including hepatosomatic index (HSI), liver glycogen, serum glucose/total protein, serum osmolarity, concentrations of sodium and chloride ions and the expression of heat shock protein 70 (HSP70). The results showed that HSI, liver glycogen content and HSP70 expression, and serum total protein decreased significantly after fasting for 4 and 7 days (p<0.05). However, no change in serum glucose, [Na+] and [Cl-] was found. Our results revealed that fasting stress in 7 days disturbs multiple physiological responses of Asian seabass and provide related information for evaluating the effect of fasting stress on aquaculture fish species.
Shing, Wu Ming, and 吳明賢. "Study of infectious liver necrosis virus on barramundi (Lates calcarifer)." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/79574977207934129250.
Full text輔仁大學
生命科學系碩士班
92
The first case of suspected viral infection of the giant seaperch (Lates calcarifer, Bloch ) fry, which caused 50-90% mortality, was reported in Thailand in 1970, and cases of viral infections of the cultured giant seaperch followed in Australia and Southeastern Asia. The first virus infection in farmed giant seaperch was reported in Taiwan in 1993 and cases occurred continuously. In 2001, the farmed giant seaperch in south Taiwan appeared poor appetite, ataxia and death. We sampled and dissected some ill fish and found extensive hemorrhagic areas and swollen on the anterior liver, which contained lots of cavities histologically. Under the transmission electronic microscope, there were 160-200 nm hexahedral viral particles, whose electronic compact center was 100-120 nm, and 10 to 20 particles aggregated in tissues. We homogenized the hepatic tissue of ill fish, removed the pellet by centrifugation, filtrated the supernatant through 0.22 m filters, and obtained the fish tissue extract. As we injected the tissue extract into healthy giant seaperch, cavities in hepatic tissues were observed. We tried to replicated the giant seaperch virus from the established grouper cell lines, however, they were not susceptible to the virus containing hepatic extract, and we had to establish giant seaperch cell lines for virus replication. We cultured cells from brain, heart, liver, kidney, spleen and muscle, and obtained four types of giant seaperch brain (sp b) cells, three flat and one spindle, and kidney (giant seaperch kidney, sp k) cells, swim bladder (giant seaperch swim bladder, sp b) cells, as well as muscle (giant seaperch muscle, sp m) cells, which appeared spindle. Characterization of cells revealed that cell lines sp b-7 and sp b-2 showed the best growth rate as cultured in the medium containing 15% fetal bovine serum and incubated at 36 °C. Chromosome analysis of cell line sp sb-2 showed that its chromosome number was 40 to 46. The susceptibility study of giant seaperch cell lines to virus from the liver extract of ill fish showed that sp sb cells appeared cytopathic effect (CPE) as incubated with 101- to 103-fold dilutions of virus. However, no significant CPE observed in other giant seaperch cell lines. To detect virus from the tissue extracts of ill fish and cell extracts which showed CPE by PCR, we designed primers referred to the frog virus 3 (FV3) major capsid protein, however, no PCR product was amplified. It is suggested that this giant seaperch virus is not FV3 related or the virus amount in the tissue extract is not sufficient for PCR amplification.
Chen, Jian-Chih, and 陳建誌. "Preparation and Functionality of Collagen Peptide from Silverperch (Lates calcarifer)." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/28422035806417199746.
Full text國立高雄海洋科技大學
水產食品科學研究所
99
The purpose of this study is to establish the process conditions for preparing collagen peptide from seaperch scale and to study its functionality on calcium-binding (Ca-B) activity. Powder samples from fish scale of seaperch were treated with alcalase, flavourzyme, papain, trypsin, and protamex. The collagen peptides obtained were examined and compared with their Ca-B activity. Among them, peptides from fish scales treated with alcalase, flavourzyme, papain showed higher Ca-B activity, and were selected in the following study. The seaperch scale was then treated with two enzymes system, including alcalase plus flavourzyme and papain plus flavourzyme and alcalase plus papain. Collagen peptide from fish scale treated with alcalase plus flavourzyme showed higher Ca-B activity. The optimal conditions in two enzymes system were listed as follows : concentration of fish scale, 6%; temperature 50 oC; pH 7.5; reaction time, 2 h; alcalase used, 8%, flavourzyme, 6%. Collagen peptides obtained from the above two enzymes system were purified with hydroxyapatite column separation and Q FF column chromatography. Two fractions named Hpt-1, Hpt-2 were obtained from hydroxyapatite column separation, Hpt-2 showed higher Ca-B activity than that of Hpt-1. Hpt-2 was then purified twice with Q FF column chromatography. Three fractions of QFF-1, QFF-2, QFF-3 were found in the chromatogram. Among them, QFF-2 showed the highest Ca-B activity. The QFF-2 was examined with HPLC and showed only one peak in the chromatogram. Analysis on the sequence of amino acid in QFF-2 is being investigated.
Xue, Yi-Jing, and 薛宜靜. "Determining the Anesthetic Dose of Tricaine Methanesulfonate Used in Sea Bass (Lates calcarifer) and Residue Test in Sea Bass (Lates calcarifer) and Grouper (Epinephelus malabaricus)." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/d8k266.
Full text國立嘉義大學
農業科學博士學位學程
106
Although tricaine methanesulfonate (MS-222) is often used to tranquilize fish, the guidelines for its use in sea bass, a brackish species, have not been established. The aim of the study reported here was to establish the tranquilizing concentration of MS-222, based on the time required for MS-222 residue elimination and withdrawal. Thirty-six fish (6/group) were immersed in different concentrations of MS-222 (0, 30, 50, 60, 70 and 90 μg/mL) to evaluate the fish physiological behavior. Optimal sedation concentration is 30 μg/mL, and optimal anesthetic concentration is 90 μg/mL. After 200 fish were anesthetized at 90 μg/mL, the fish achieved a healthy recovery within 72 h after the administration of saline. The 10 fish in the control group were subject to the same treatment without anesthesia, 3 out of 10 died. After 108 fish (54/group) were immersed in 30 or 60 μg/mL of MS-222, the sedated fish were healthy during and after the 8 h of transport. However, all the 10 fish in the control group died within 3 days. By high performance liquid chromatography, the residue of MS-222 in sea bass was assessed. In the skinned muscle and liver, the elimination half-life was 5.54 and 5.27 h (30 μg/mL) and 8.72 and 7.15 (60 μg/mL), respectively, and the withdrawal time was at least 4.5 days at 30 μg/mL and 7.5 days at 60 μg/mL. By liquid chromatography tandem-mass spectrometry, the residue of MS-222 in grouper was assessed. In the 30 μg/mL group, the skinned muscle not detect at fifth day, and the liver at day 21 detected 0.005 µg/g. In the 60 μg/mL group, the skinned muscle not detected at day 14, and the liver at day 21 detected 0.007 µg/g.
Matthews, Sue Janet. "The regulation of insulin-like growth factors in barramundi, Lates calcarifer." Thesis, 1997. https://researchonline.jcu.edu.au/33782/1/33782-matthews-1992-thesis.pdf.
Full textBudd, Alyssa. "Epigenetic effects of temperature on sex change in barramundi, Lates calcarifer." Thesis, 2020. https://researchonline.jcu.edu.au/65689/1/JCU_65689_budd_thesis_2020.pdf.
Full textHSIEH, HUI-SHU, and 謝慧淑. "Preparation of peptide-Calcium complexes with hydrolysates of Perch(Lates calcarifer) scale." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/98533053322040456677.
Full text國立高雄海洋科技大學
水產食品科學研究所
105
The objective of present work is to study the process conditions for preparing peptidechelated calciumcomplexes (Peptide-Ca)from collagen peptide of perch scale. Collagen peptides were first obtained from perch scale treated with alcalase plus flavourzyme at 50 oC, pH 7.5 for 2 hrs.The optimal conditions for preparing Peptide-Cawere listed as follows: reaction temperature 50 oC at pH 7.5 for 2 hrs.; ratio of peptide to calcium chloride, 2: 1 and calcium chelating activity was 68.4 ± 0.41%. The obtained Peptide-Ca showed good resistance to the treatment of artificial digestive fluid.Methanol and ethanol showed good extraction efficiency in the purification of Peptide-Ca. Main chemical bond inPeptide-Ca was found to be COO− and calcium ion which was identified by Fourier Transform Infrared Spectrometer (FTIR). Among the amino acids of scale collagen pepdide, proline showed the highest efficiency in chelating calcium ion, followed by glycine and hydroxyproline. Hdrolysates from perch scale, tilapia scale and horseshoe shell, all showed good calcium ion chelating activity, the highest was that from horseshoe shell, followed by perch scale and tilapia scale. Moreover,hydrolysates of perch scale also exhibited other metal ion chelating activity, the highest one was Zn2+, followed by Fe2+, Ca2+, and Cr3+, Mg2+.
Collins, Geoffrey M. "Phenotypic drivers of hypoxia tolerance in a tropical diadromous fish (Lates calcarifer)." Thesis, 2016. https://researchonline.jcu.edu.au/48866/1/48866-collins-2016-thesis.pdf.
Full textHuang, Chia-Chi, and 黃佳琪. "The study of CpG motifs induced cytokines production in sea bass (Lates calcarifer)." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/83873593317259750124.
Full text嘉南藥理科技大學
生物科技系暨研究所
96
The effects of unmethylated CpG oligodexynucleotides (ODNs) on the mammalian immune system are relatively well studied. CpG ODNs have been shown to induce a variety of different immune responses in fish including IFN ??? induction along with antiviral activity, macrophage activation, cytokine production and activation of other immune-related genes and cell proliferation. CpG ODNs can indirectly inducing cellular migration in vitro and enhancing serum lysozyme activity in vivo. Sea bass (Lates calcarifer), a common economical aquaculture species in south Taiwan, was chosen as the objective in this study to investigate cytokines induced by CpG ODNs. Previous studies showed that CpG ODNs can induce trout head kidney cells to produce proliferation inducing factors (PIFs), and then result in cell proliferation. However, B cells depletion did not affect the responses of trout head kidney cells to CpG ODNs stimulation. These results indicate that neither the source of the proliferation-inducing factors or the proliferating responder cells are B cells. The aims of our study are to find the source of PIFs and their target cells. Furthermore, we would try to purify and identify of the proliferation-inducing factors. Our results showed PIFs were proteins secreted by CpG ODNs stimulated phagocytes, and PIFs induced a subpopulation of lymphocyte to proliferate. Besides, phagocytes were supposed to act as accessory cells in this response.
Gamage, Kumudu Radampola. "The effect of nutrition on reproductive parameters in male barramundi, Lates calcarifer (Bloch)." Thesis, 2001. https://researchonline.jcu.edu.au/33768/1/33768-gamage-2001-thesis.pdf.
Full text"Effects of sublethal nitrite concentrations on the metabolism of the sea bass, Lates calcarifer." Chinese University of Hong Kong, 1989. http://library.cuhk.edu.hk/record=b5886165.
Full textTseng, Tzu-Yuan, and 曾梓淵. "Studies on the Protective Effectiveness of Streptococcus iniae Killed Bacterial Vaccine in Lates calcarifer." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/67508152880864133910.
Full text國立高雄海洋科技大學
水產養殖研究所
102
Currently, Streptococcus iniae (S. iniae) has become global aquaculture pathogen. The pathogen will infect fish and cause serious death and economy cost. This is a big threat to aquaculture. Those infected fish would have following symptoms: erratic swimming, darkening of the skin, exphthalmia, cornel opacity, ascites, peritonitiss, enlargement of spleen, petchiae around the anus etc. This study is mainly for prepare Streptococcus iniae killed bacterial vaccine to injective and oral immune for Lates Calcarifer. Injection of immune are S. iniae killed bacteria vaccine, intraperitoneal injection at an average weight of 100g of Lates calcarife. Injection concentrations are 1×107, 1×108, 1×109 CFU/250μl/fish for three times. Oral immune as dead bacteria mixes granular feed, concentrations are 1×108, 1×109, 1×1010 CFU/ml/g, continuous feeding of 4 weeks. After immunization use 1×103 CFU/250μl/fish LD50 to challenge. Pre-immune, immune 2-week, 4-week, after challenge and 18-week take blood , applied ELISA, SDS-PAGE and Western Blot to antibody reaction.The results show for injection of immune concentrations in 1×108, 1×109 CFU/250μl RPS are 100%, in 1×107 is 80%, and oral immune RPS ranged from 20% to 40%. This means these two immunizations are effective. Although they both have been tested the existence of antibody, the consequence shows that injective immunization is working better. The results of ELISA reaction reaches peak in the third time of enhancing immunization. After use SDS-PAGE, with Lates calcarife serum speculate 72kDa and 25kDa. Thus, we could speculate 7、26、40、45、55 and 85kDa are important source of immune in western blot. Based on this experiment, we could use Streptococcus iniae killed bacterial vaccine on injective immune to be a direction of future development.
Chang, Jia-Rong, and 張家榮. "Establishment of a continuous cell line derived from barramundi (Lates calcarifer ) and its applications." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/52636999813684799231.
Full text國立宜蘭大學
生物技術研究所碩士班
95
Lates calcarifer (bloch) belongs to the Lates genus, Centropomidae family, Perciformes order and Osteichthyes class. This tropical and sub-tropical fish is located in the coastal brackish water throughout Okinawa to the Indian Ocean. Lates calcarifer is massively cultured in Southeastern Asia and Southern Taiwan. Nervous Necrosis Virus (NNV), a member of the Betanodaviridae, is an icosahedral, non-enveloped RNA virus 25-30 nm in diameter. NNV mainly infects juveniles, destroying the nerves of the brain, leading to abnormal swimming and high mortality of infected fish, causing devastating economic impact to the aquaculture industry. Since the larvae of Lates calcarifier are highly susceptible to NNV, this research study developed primary and continuous cell lines from the liver, kidney, and the gas bladder of bloch. The liver and kidney cells were propagated over 90 passages while the gas bladder cell line was propagated over 150 passages, maintained in 5% FBS, L-15 medium, and grew quickly at both 28°C and 32°C. Epinephelus lanceolatus NNV was first used to test these different cell lines for susceptibility and results showed that Lates calcarifer gas bladder (LCGB) was highly susceptible. Then, wild type Plectropomus leopardus Nervous Necrosis Virus (PLNNV) was replicated in LCGB and the viral titer was 107 TCID50/mL. Large quantities of PLNNV was grown in LCGB, collected and purified, serving as the antigen for anti-NNV monoclonal antibodies production in BALB/c mice. After limiting dilution and screening, 3 different hybridomas secreting monoclonal antibodies against NNV were successfully produced. The 3 monoclonal antibodies were confirmed by Western blot and IPMA against purified NNV. Subsequently, cloning of PLNNV capsid gene and expression of recombinant proteins in E. coli was done in pQE30 vector and M15 host. A Western blot of the monoclonal antibodies against the recombinant protein proved all 3 monoclonal antibodies produced in this study were against the NNV capsid protein. These monoclonal antibodies are important tools to have for further researches on Nervous Necrosis Virus.
Ngo, Diu Thi. "Evaluation of canola meal as an aquafeed ingredient for barramundi (Asian seabass; Lates calcarifer)." Thesis, 2014. https://researchonline.jcu.edu.au/41354/1/41354-ngo-2014-thesis.pdf.
Full textHsu, Shu-Ting, and 許淑婷. "Cloning and tissue expression of peroxisome proliferator-activated receptor α in Asian seabass, Lates calcarifer." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/45010093003193643172.
Full text國立嘉義大學
水生生物科學系研究所
97
Peroxisome proliferator activated receptors (PPARs) are a family of hormone receptors implicated in a collection of fundamental biological processes, mainly related to lipid metabolism regulation. Three subtypes of PPAR, termed α, β and γ have been identified in several vertebrate species. The aim of present study was to �� clone the PPARα gene and �� examine the gene expression of PPARα in various tissues and the related with body composition in different body size of Asian seabass (Lates calcarifer). �� A 256-bp cDNA fragment of PPARα was cloned from Asian seabass by reverse transcription polymerase chain reaction (RT-PCR) using specific oligonucleotide primers which were designed according to known evolutionary conserved sequences of certain PPARα domains, available at NCBI. A comparison between the correspondent PPARα cDNA sequences of the Japanese seaperch (Lateolabrax japonicus), red seabream (Pagrus major), Japanese pufferfish (Takifugu rubripes), house mouse (Mus musculus) and human (Homo sapiens) revealed 93%, 92%, 87%, 79% and 77% identity, respectively. �� Expression of PPARα gene in various tissues and different body size ranged from 3.34 g to 8.03 g and 618 g of Asian seabass were analyzed. Expression of PPARα gene in 3.34 g to 8.03 g of juvenile Asian seabass was detected in the liver, spleen, heart, kidney, brain, pyloric caeca, intestine, dorsal muscle and gill. It showed abundant in the pyloric caeca and intestine, and at a barely detectable level in spleen and gill. Expression of PPARα gene in marketing size (618 g) of Asian seabass was detected in the liver, spleen, heart, head kidney, trunk kidney, brain, pyloric caeca, intestine, dorsal muscle, ventral muscle and gill. It showed abundant in trunk kidney, spleen, heart and brain, and at a lower level in liver, dorsal muscle, ventral muscle and gill. Proximate analyses showed that a negative correlation between the crude lipid level, hepatosomatic index and body weight was found (p<0.05). In conclusion, the 256 bp cDNA fragment of PPARα in Lates calcarifer has been cloned. The highest expression of PPARα gene was found in pyloric caeca and intestine in juvenile Lates calcarifer with body weight of 3.34 g to 8.03 g. It showed abundant in spleen, heart, trunk kidney and brain in marketing size (618 g) of Lates calcarifer.
Hu, Hsiulong, and 胡秀龍. "The economical benefit analysis of the commercial specification of Giant seaperch (Lates calcarifer) aquaculture industry." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/50134602815436227920.
Full text國立高雄海洋科技大學
漁業生產與管理研究所
99
This study aims to discuss the current Taiwan sea bass aquaculture production costs and market specifications of management efficiency, and carry out the hypothetical analysis to investigate the market specification as 0.6 kg/individual , 1.2 kg/individual and 1.8 kg/individual of selling points in order to provide farmers more practical farming operation concept.The research in 2009-2010 found that production cost increase significant was in feed expenditure, other costs (cost of water and electricity, personnel, fish breeding cost) were no different. Statistical results on operating in market specifications, the cost variance can be classified into three points as follows: 1.Results of cost imputs analysis: feed and the personnel cost will be due to feeding 1.8 kg/individual specification and has significantly differences, but remaining cost is no significantly differences (p>0.05). It explains that culture process in the cost of water and electricity and the fish breeding costs, 0.6 kg/individual, 1.2 kg/individual and 1.8 kg/individual of specifications has no significantly differences, and has not show different whatever in culture time elongated of process. 2. Results of the profit ratio analysis: market specification as 0.6 kg/individual and 1.8 kg/individual o seedlings have significant differences in fish breeding cost, the remaining personnel, feed, and water and electricity of benefits have no significant difference (p>0.05). 3. Results of productivity analysis: fish breeding productivity has significant different in market specifications (p<0.05).In the other words the market specifications on the fish breeding production sold in which 1.8 kg/individual is higher than that of 1.2 kg/individual and 0.6 kg/individual, and that of 1.2 kg/individual is higher than of 0.6 kg/individual while there is no significant difference in personnel, feed, and water and electricity productivity (p >0.05). Secondly on the analysis of the hypothetical, when 1.8 kg/individual fixed profit at $ 81.25/kg, we discuss the fish breeding profit ratio, total expenditure ratio and net profit, the results of the analysis are as follows: 1. In fish breeding profit ratio results , the 0.6 kg/individual and 1.2 kg/individual could be sold $ 98 and $ 115.5, respectively and has no difference in price statistics. 2. In total expenditure ratio results, the 0.6 kg/individual and 1.2 kg/individual could be sold $ 82 and $ 85.2, respectively and has no difference in price statistics. 3 .In net profir results, the 0.6 kg/individual and 1.2 kg/individual could be sold $ 103.3 and $ 126.4, respectively and has no difference in price statistics.
Athauda, A. R. Saman Bandara. "Effect of culture environmental conditions on sex inversion of Asian seabass (barramundi), Lates calcarifer (Bloch)." Thesis, 2014. https://researchonline.jcu.edu.au/45404/1/45404-athauda-2014-thesis.pdf.
Full textHuang, Ying-Chang, and 黃盈昌. "Analysis the Lates calcarifer growth rate applied replacement of fish meal by various fermented soybean meal." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/88449419291595683781.
Full text國立高雄海洋科技大學
水產養殖研究所
101
The study was aimed to find out analysis the Lates calcarifer growth rate applied replacement of fish meal by various fermented soybean meal. We are going to divide into two stages of experiments and use the fermentation feed which are fermented soybean meal 1, fermented soybean meal 2 and fermented soybean meal 3 to replacement of fish meal. The first phase were divided into A, B and control groups, a total of three groups, four repeat, control group feed commercial formula, and A, B group fermented soybean meal 1 and fermented soybean meal 2 replace 20% fishmeal in commercial formulations. The second phase were divided into groups A, B, C and control group, a total of four groups, three repeated,where A, B and control group renewal of the first stage of the experiment, C group fish out by the control component and replaced 20% fermented soybean meal 3 of the commercial formulations fishmeal feeding trials. Feeding time at every stage of each six weeks, and random sampling once every two weeks, weighed weight, feed for six weeks, the experimental data, we also use the SPSS software to be the experimental data. According to the statistical, the result display the A, B group compare with control group, gain weight, feed intake and feed conversion rate haven’t significantly different(p>0.05). However, compare with A and control group, the specific growth rate are significantly different (p<0.05), C group haven’t significantly different too. The second stage, the result display the A, B, C group compare with control group, gain weight and feed conversion rate haven’t significantly different(p>0.05). But A, B and C group compare with control group, those specific growth rate and feed intake are significantly different(p<0.05). After testing, in measuring Viscerosomatic index and the length of the intestinal microvillus. Viscerosomatic index A, B, Cgroup compare with control group have significantly different (p<0.05), and A, B, C group between have significantly different, index B group> C group> A group> control group; the length of the intestinal microvillus especially the preceding part of m icrovillus bigger than posterior segment microvillus, preceding part of microvillus A, B, C group compare with control group have significantly different (p<0.05), and A, B, C group between have significantly different, length of B group> A group> C group> control group, posterior segment microvillus A, B, C group compare with control group haven’t significantly different(p>0.05). Spine bone analysis, zinc and phosphorus content is normal in the Lates calcarifer . The above results feed fermented soybean meal to replace 20% fishmeal is no effect on the growth of Lates calcarifer.
Nankervis, Leo. "Quantitative and qualitative aspects of the protein nutrition of barramundi (Lates calcarifer) larvae fed formulated foods." Thesis, 2005. https://researchonline.jcu.edu.au/1317/1/01front.pdf.
Full textNankervis, Leo. "Quantitative and qualitative aspects of the protein nutrition of barramundi (Lates calcarifer) larvae fed formulated foods /." 2005. http://eprints.jcu.edu.au/1317.
Full textMuirhead, Elisabeth Knowles. "Polybrominated diphenyl ethers: levels in Townsville sediments, depuration and (anti-)estrogenic effects in Barramundi (Lates calcarifer)." Thesis, 2008. https://researchonline.jcu.edu.au/4778/1/Thesis_front.pdf.
Full textMarc, Adrien François. "Development of advanced reproductive techniques to characterize fertility and accelerate selective breeding in barramundi (Lates calcarifer)." Thesis, 2021. https://researchonline.jcu.edu.au/74221/1/JCU_74221_Marc_2021_thesis.pdf.
Full textKuo, Chi-Wei, and 郭紀偉. "Pharmacokinetic and Residual Time Study of Oxolinic Acid in the Sea Fish Trachinotus blochii and Lates calcarifer." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/61487831831032376306.
Full text國立臺灣大學
獸醫學研究所
99
The sea water used for fish culture instead of fresh water in the west-south sea shore of Taiwan was very common. Owing to the land fish pond was not wide, the aquaculture in high density caused the high risks for suffering several diseases. In order to prevent the diseases of fish, not only improving the cultivating environments and techniques, but also tried to use various chemical therapy drugs in clinical use. Taiwan is located in the subtropical area, the water temperature was so often warm that the fish always suffered from viral, bacterial infected diseases, others such as parasite, protozoa, alga and mould were also infestation in fish. An experimental trial was performed to determine the half life of oxolinic acid in sea fish Trachinotus blochii and Lates calcarifer of pharmacokinetic and residue studies. A single dose pharmacokinetic study of oxolinic acid was applied by oral administration at the dose of 60 mg/kg b.w./day. A high performance liquid chromatographic method based on fluorescence detection was developed for determination of oxolinic acid in fish blood, tissue samples. Oxolinic acid in sea fish Trachinotus blochii of peak concentration (Cmax) was obtained directly in the concentration time profile; the residue levels in blood, muscle, liver, and kidney samples after 0.5 h depletion were at the maximum concentration. The Cmax in blood was determined to be 0.43 mg/mL and the half life was calculated to be 50.58 h. The Cmax in muscle was determined to be 1.43 mg/g and the half life was calculated to be 25.76 h. The Cmax in liver was determined to be 1.42 mg/g and the half life was calculated to be 45 h. The Cmax in kidney was determined to be 3.14 mg/g and the half life was calculated to be 25.76 h; Oxolinic acid in sea fish Lates calcarifer of Cmax was obtained directly in the concentration time profile; the residue levels in blood, muscle, liver, and kidney samples after 1 h depletion were at the maximum concentration. The Cmax in blood was determined to be 1.55 mg/mL and the half life was calculated to be 170.69 h. The Cmax in muscle was determined to be 2.55 mg/g and the half life was calculated to be 84.51 h. The Cmax in liver was determined to be 8.18 mg/g and the half life was calculated to be 106.61 h. The Cmax in kidney was determined to be 4.72 mg/g and the half life was calculated to be 111.77 h. An experimental trial was performed to establish an adequate depletion period of oxolinic acid in sea fish Trachinotus blochii and Lates calcarifer of pharmacokinetic and residue studies. The pharmacokinetics of oxolinic acid was applied by oral administration at the dose of 60 mg/kg b.w./day, and was given at a daily dose for five consecutive days. A high performance liquid chromatographic method based on fluorescence detection was developed for determination of oxolinic acid in fish blood, muscle, liver and kidney. Data showed that the average residue levels in sea fish Trachinotus blochii of blood, muscle and kidney decreased to 2.74 ng/mL, 13.82 ng/g, and 21.53 ng/g after 24 days post dosing, respectively; with one exception at 195.01 ng/g in liver tissue. The present study provided data on the depletion of oxolinic acid residues in sea fish Trachinotus blochii after oral administration of five doses of the drug. The residue levels after 24 days depletion decreased to below the maximum residue limits (MRLs) after last oral treatment. At the dosage of 60 mg/kg b.w./day, we suggested that the recommended withdraw period was 36 days; Data also showed that the average residue levels in Lates calcarifer of blood muscle, liver and kidney decreased to MRLs after 2, 12, 36, and 36 days post dosing, respectively. The present study provided data on the depletion of oxolinic acid residues in Lates calcarifer after oral administration of five doses of the drug. The residue levels after 12 days depletion decreased to below the MRLs after last oral treatment. At the dosage of 60 mg/kg b.w./day, we suggested that the recommended withdraw period was 18 days.
Chen, Yen-Chun, and 陳讌君. "Characterization of apoptosis induced by grouper iridovirus in two newly established cell lines from Barramundi (Lates calcarifer)." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/01425744912026635231.
Full text國立宜蘭大學
生物技術研究所碩士班
96
This study demonstares the induction of apoptosis in BM (barramundi muscle) and BSB (barramundi swim bladder) cell lines, by grouper iridovirus (GIV) infection. The apoptosis was detected and confirmed through various methods. At MOI of 10, cytopathic effect (CPE) characterized as cell shrinkage and rounding was first observed in both GIV-infected BM and BSB cells at as early as 45 min pi (post-infection). GIV infection appeared to progress more rapidly in BM cells than in BSB cell as suggested by the faster development of CPE in the infected BM cells. Cell viability assay also showed a stronger inhibitory effect on the BM (26.6%) and BSB (54.8%) cells by GIV at 4 hpi. The DNA fragmentation, Annexin V and Hoechst 33258 assays were performed at 45 min pi. The chromatin nick could be observed by TUNEL assay at 2 hpi. The above results demonstrate that BM and BSB cell lines can be triggered apoptosis by GIV. The heat-inactivated GIV and UV-inactivated GIV were used to infect cells. The results showed only UV-inactivated can induce apoptosis. Caspase-3, -8, and -9 activities in the BM- and BSB-infected cells were early activated at 30 min pi., and as the infection goes along, caspase-3, -9 had the maxima activities at 45 min pi. However, caspase-8 had the maxima activity in comparison to the mock-infected cells at 1 hpi. GIV-induce apoptosis was inhibited by a pan-caspase inhibitor, z-VAD-fmk, indicating a caspase-activation pathway. The above results showed that GIV can induce BM and BSB cells apoptosis and the apoptosis pathway is caspase-activation dependent pathway.
Chen, Chin-Wen, and 陳瑾玟. "Study On The Genes Expression of Barramundi (Lates calcarifer)Muscle Cell Line Induced By Grouper Iridovirus Infection." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/12272147415756602881.
Full text國立宜蘭大學
生物技術研究所碩士班
96
By definition, viruses are unable to replicate within a host cell. Virus infection can trigger events that lead to apoptosis, however some viruses are known to encode gene products or induce host gene products that block apoptosis and use the host-cell macro-molecular machinery and energy supplies to replicate. In previous studies, it was suggested that GIV could induced apoptosis in barramundi muscle cells via caspase-3, -8, -9 activation pathway. In this study, a proteomic method was applied to identify alterations in protein expression profile in barramundi muscle cells and being an indicator of apoptosis after GIV infection. From this screening, 7 novel proteins were identfied, including Initiation factor 4A 1B (eIF4A1B), Proteasome 20S, ADP-ribosylation factor 1 (Arf-1), Ribose 5 phosphate isomerase(RPIA), Tyrosine Hydroxylase(TH), RAN binding protein 1, and Phosphoglycerate mutase 1(PGAM1), were overexpressed in GIV-infected barramundi muscle cells. In addition, we also determined these genes expression levels by real-time PCR. The results showed that except Initiation factor 4A 1B (eIF4A1B)but, ADP-ribosylation factor 1(Arf-1), Ribose 5 phosphate isomerase (RPIA)and Tyrosine Hydroxylase (TH)were up regulated conpared to the mock-infected cells. However, the Phosphoglycerate mutase 1(PGAM1) expression only rises slightly is heightened in comparison to the mock-infected cells. Summarized our results indicated that GIV could induce host genes expression level, by GIV infection and supports viral, and replication virus-infected cells of premature death. Our results provide an information for understanding of GIV viral replcation in the host cells and possible mechenisms apoptosis regulatory systems.
Wei, Song, and 宋瑋. "Study on the Application of Nucleotides Product on Development of Low-fish Meal Feed in Lates calcarifer." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/81396198931847258018.
Full text國立高雄海洋科技大學
水產養殖研究所
102
This study assessed the influence of adding commercially available nucleotides and various ratios of soybean meal to replace fishmeal as feed on the growth rates and survival of Lates calcarifer. The experiment involved 6 groups, which included fish that were fed basic feed, feed that comprised soybean meal that replaced 40% or 80% of fishmeal, feed that was supplemented with commercially available nucleotide 0.2% Vannagen, 0.1% Vannagen, or 0.05% Nutrafito Plus, or generic, commercially available sea bass feed. The addition of appropriate nucleotide amounts was expected to reduce the amount of fishmeal necessary, thereby lowering feed costs. The results indicated that the addition of soybean meal and commercially available nucleotides did not substantially increase the rates of specific growth and weight gain. However, the superoxide dismutase immunoreactivity was higher in the feed that included commercially available nucleotides than those that did not (p <0 .05). This result showed that the fish that were given feed supplemented with nucleotides demonstrated an improved ability to remove free radicals, thereby enhancing the immunoreactivity and increasing the survival rate of sea bass. Numerous studies have indicated that adding nucleotide to feed can elevate the immunoreactivity of fishery products, and this study presented similar results. Among the feeds used in the experiment, the costs of the feeds without nucleotides were the lowest. However, because the prices of fishmeal are increasing, adding nucleotides to feeds can reduce costs in the future. Nevertheless, the appropriate amount of nucleotides to be added must be further verified by conducting further experiments.
Chern, Tzuoo-Jenq, and 陳佐政. "Effect of thermal stress and chlorine residue on the physiological response of the giant seaperch, Lates calcarifer." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/91783672007479786169.
Full text國立屏東科技大學
水產養殖系
92
This research discusses the acclimation responses and physiological change, focused on the energetics, of the giant seaperch, Lates calcarifer, under different thermal stress and remnant chlorine concentration environment. The tolerance and adaptive response under this dual adverse circumstance, becomes the basis of environmental impact evaluation. In gentle warming pressure, giant seaperch dies in the water temperature 42~42.4 ℃; In sublethal tolerance experiment, giant seaperch is really resistant to the hypochlorous acid pressure below the water temperature 34 ℃. High survival rate was obtained under chlorine concentration up to 2 mg/ l, but the rate obviously decline when the water temperature is 38 ℃. In blood biochemistry analysis, there are no significant difference of blood glucose change physiologically under water temperature 25~34 ℃. For 38 ℃ group, it is extremely obvious high in blood glucose concentration. The blood lactic acid quantity has rises under the different water temperature, also degree of the rise is becomes the relevance change scope with the temperature. Water temperature and chlorine residue, both contribute the stress for giant seaperch but in different mechanism. The water temperature has created a physiological reaction hyperglycemia, but the chlorine residue actually has the suppression effect on energy metabolism, also its suppression is positive proportional to the remnant chlorine concentration. Changes in blood lactic acid concentration appears is the early time of stress, also proportional to the intensity of stimuli, including thermal elevation and chlorine residue increasing. The concentration of blood lactic acid is suitable for being stress response index as its sensitivity to stressors. In muscle, difference and change of glucose and glycogen content did not show any significant varience within short time. But the lactic acid accumulation in muscle, reveal the physilogical stress caused by the existence of chlorine residue, which indicated energy supply by anaerobic metabolism while giant seaperch is in urgency or burst muscular motion. In liver, however, nearly contains no lactic acid and it is not feasible for monitoring the physiological response of stress reaction. Liver glycogen content demonstrated the chlorine residue suppression on energy metabolism, but under the identical chlorine concentration, the glycogen content change did not reflect the different thermal treatments, no statistic significance obtained. Consumption of energy for physiological regulation resulted in glucose concentration declined and immediately recovered in liver, explained the energy source comes from glycolysis or fatty acid oxidation but glycogenolysis, which changed the glucose content.
Newton, James Raymond. "Investigating the genetics of thermal tolerance and adaptation to temperature amongst populations of Australian barramundi (Lates calcarifer)." Thesis, 2013. https://researchonline.jcu.edu.au/41078/1/41078-newton-2013-thesis.pdf.
Full textLi, Yao-Lin, and 李曜伶. "Strain Variation in Nocardia seriolae Fish Isolates and Immune Response of Asian Seabass ( Lates calcarifer) to Nocardia seriolae." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/81208517337322571253.
Full text國立屏東科技大學
水產養殖系所
98
Nocardia seriolae is the causative agent of nocardiosis in cultured fish and causes high mortality in Taiwan and Japan. In this study, N. seriolae was isolated from 14 species of fish in Taiwan and Japan during 1970 to 2009, and Fourty-one N. seriolae strains were identified by 16Sr DNA gene, hsp gene and rpoβ gene analysis. The variations of 16S rDNA, hsp and rpoβ gene of N. seriolae were 0.2% ( 5 Taiwan strains ), 0.2~0.7% ( 6 Taiwan strains, 2 Japanese strains)、0.2~0.7% (6 Taiwan strains, 3 Japanese strains) isolated from ten fish species from 1986 to 2007, respectively. Further study evaluates the in vitro activity of antimicrobial agents against 89 strains of N. seriolae (67 Taiwan strains and 22 Japanese strains). The 50 and 90% MICs values for 89 strains of N. seriolae of the antimicrobial agents used in this study, florfenicol, lincomycin, doxycyclin, oxytetracycline, and kitasamycin were the most active antimicrobial agents against the isolates tested, followed by erythromycin and spiramycin. Furthermore, in an attempt to develop a vaccine against N. seriolae 961113, microfluidizer antigen (MF), and microfluidizer antigen mixed with ISA 763A adjuvant were injected into Asian seabass, Lates calcarifer. Fish were challenged with N. seriolae 961113 and 980401-2 after immunized 4 weeks and 14 week, respectively. RPS values of MF and MF-ISA 763A group challenged by N. seriolae 961113 at 4 weeks after immunization were at 67% and 83%, respectively. However, RPS values of MF and MF-ISA 763A group challenged by 980401-2 strain at 14 week after immunization were at 0% and 67%. A immunostimulatory but not protective in Asian seabass was observed in immunized group challenged by N. seriolae 980401-2 and 961113 strain at 4 weeks and 14 weeks, respectively. These results suggest that cross protective immune responses against N. seriolae are induced in Asian seabass.
Trujillo, González Alejandro. "Behaviour, histopathology and immunobiology: interactions between the ectoparasite Neobenedenia (Monogenea: Capsalidae) and its host, Lates calcarifer (Perciformes: Latidae)." Thesis, 2015. https://researchonline.jcu.edu.au/43749/1/43749-trujillo-gonzalez-2015-thesis.pdf.
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