Relevant bibliographies by topics / LAMP2a

Academic literature on the topic 'LAMP2a'

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Published: 6 July 2024

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Journal articles on the topic "LAMP2a":

1

Cuervo, A. M., and J. F. Dice. "Unique properties of lamp2a compared to other lamp2 isoforms." Journal of Cell Science 113, no. 24 (December 15, 2000): 4441–50. http://dx.doi.org/10.1242/jcs.113.24.4441.

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Lamp2a acts as a receptor in the lysosomal membrane for substrate proteins of chaperone-mediated autophagy. Using antibodies specific for the cytosolic tail of lamp2a and others recognizing all lamp2 isoforms, we found that in rat liver lamp2a represents 25% of lamp2s in the lysosome. We show that lamp2a levels in the lysosomal membrane in rat liver and fibroblasts in culture directly correlate with rates of chaperone-mediated autophagy in a variety of physiological and pathological conditions. The concentration of other lamp2s in the lysosomal membrane show no correlation under the same conditions. Furthermore, substrate proteins bind to lamp2a but not to other lamp2s. Four positively-charged amino acids uniquely present in the cytosolic tail of lamp2a are required for the binding of substrate proteins. Lamp2a also distributes to an unique subpopulation of perinuclear lysosomes in cultured fibroblasts in response to serum withdrawal, and lamp2a, more than other lamp2s, tends to multimerize. These characteristics may be important for lamp2a to act as a receptor for chaperone-mediated autophagy.
2

Fukushima, Masaya, Tatsuya Inoue, Takashi Miyai, and Ryo Obata. "Retinal dystrophy associated with Danon disease and pathogenic mechanism through LAMP2-mutated retinal pigment epithelium." European Journal of Ophthalmology 30, no. 3 (March 5, 2019): 570–78. http://dx.doi.org/10.1177/1120672119832183.

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Introduction: Lysosome-associated membrane protein 2 plays an important role in autophagy and lysosomal function and its mutation is responsible for pathogenesis of Danon disease, which can cause retinopathy, though its pathophysiological contribution to retinal dysfunction remains unclear. The purpose of our research is to report the first case of Japanese Danon disease retinopathy and to understand how LAMP2 dysfunction contributes to pathogenesis of retinopathy. Methods: One case underwent ophthalmic examination including slit-lamp exam, fundus imaging, visual field testing, and electroretinogram. In molecular biological study, relative messenger RNA expression levels of three splicing variants of Lamp2 or LAMP2 in wild type mouse retina and retinal pigment epithelium, human retinal pigment epithelium cell line adult retinal pigment epithelium-19 were quantified. LAMP2 was knocked down by small interfering RNA in adult retinal pigment epithelium-19 and its effect to LC3, an autophagy marker, was assessed by Western blotting. Intracellular localization of LAMP2 and LC3 in untreated and LAMP2-knocked-down adult retinal pigment epithelium-19 was analyzed by confocal microscopy. Results: Our case manifested cone dystrophy in both eyes. In mice, expression of Lamp2a and Lamp2b was significantly higher in retinal pigment epithelium than that in neural retina. Expression of Lamp2a and Lamp2b were significantly higher than that of Lamp2c in mouse retinal pigment epithelium. Adult retinal pigment epithelium-19 cells showed similar LAMP2 expression pattern to mouse retinal pigment epithelium. LAMP2 knockdown in adult retinal pigment epithelium-19 reduced LC3-II amount and the number and size of autophagosome. Discussion: We report a Japanese case of Danon disease retinopathy, and our study implies that LAMP2 plays an important role in autophagosome formation in retinal pigment epithelium.
3

Manso, Ana Maria, Sherin I. Hashem, Bradley C. Nelson, Emily Gault, Angel Soto-Hermida, Elizza Villarruel, Michela Brambatti, et al. "Systemic AAV9.LAMP2B injection reverses metabolic and physiologic multiorgan dysfunction in a murine model of Danon disease." Science Translational Medicine 12, no. 535 (March 18, 2020): eaax1744. http://dx.doi.org/10.1126/scitranslmed.aax1744.

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Danon disease (DD) is a rare X-linked autophagic vacuolar myopathy associated with multiorgan dysfunction, including the heart, skeletal muscle, and liver. There are no specific treatments, and most male patients die from advanced heart failure during the second or third decade of life. DD is caused by mutations in the lysosomal-associated membrane protein 2 (LAMP2) gene, a key mediator of autophagy. LAMP2 has three isoforms: LAMP2A, LAMP2B, and LAMP2C. LAMP2B is the predominant isoform expressed in cardiomyocytes. This study evaluates the efficacy of human LAMP2B gene transfer using a recombinant adeno-associated virus 9 carrying human LAMP2B (AAV9.LAMP2B) in a Lamp2 knockout (KO) mouse, a DD model. AAV9.LAMP2B was intravenously injected into 2- and 6-month-old Lamp2 KO male mice to assess efficacy in adolescent and adult phenotypes. Lamp2 KO mice receiving AAV9.LAMP2B demonstrated dose-dependent restoration of human LAMP2B protein in the heart, liver, and skeletal muscle tissue. Impaired autophagic flux, evidenced by increased LC3-II, was abrogated by LAMP2B gene transfer in all tissues in both cohorts. Cardiac function was also improved, and transaminases were reduced in AAV9.LAMP2B-treated KO mice, indicating favorable effects on the heart and liver. Survival was also higher in the older cohort receiving high vector doses. No anti-LAMP2 antibodies were detected in mice that received AAV9.LAMP2B. In summary, LAMP2B gene transfer improves metabolic and physiologic function in a DD murine model, suggesting that a similar therapeutic approach may be effective for treating patients with this highly morbid disease.
4

Auzmendi-Iriarte, Jaione, Maddalen Otaegi-Ugartemendia, Estefania Carrasco-Garcia, Mikel Azkargorta, Antonio Diaz, Ander Saenz-Antoñanzas, Joaquin Andrés Andermatten, et al. "Chaperone-Mediated Autophagy Controls Proteomic and Transcriptomic Pathways to Maintain Glioma Stem Cell Activity." Cancer Research 82, no. 7 (February 7, 2022): 1283–97. http://dx.doi.org/10.1158/0008-5472.can-21-2161.

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Abstract Chaperone-mediated autophagy (CMA) is a homeostatic process essential for the lysosomal degradation of a selected subset of the proteome. CMA activity directly depends on the levels of LAMP2A, a critical receptor for CMA substrate proteins at the lysosomal membrane. In glioblastoma (GBM), the most common and aggressive brain cancer in adulthood, high levels of LAMP2A in the tumor and tumor-associated pericytes have been linked to temozolomide resistance and tumor progression. However, the role of LAMP2A, and hence CMA, in any cancer stem cell type or in glioblastoma stem cells (GSC) remains unknown. In this work, we show that LAMP2A expression is enriched in patient-derived GSCs, and its depletion diminishes GSC-mediated tumorigenic activities. Conversely, overexpression of LAMP2A facilitates the acquisition of GSC properties. Proteomic and transcriptomic analysis of LAMP2A-depleted GSCs revealed reduced extracellular matrix interaction effectors in both analyses. Moreover, pathways related to mitochondrial metabolism and the immune system were differentially deregulated at the proteome level. Furthermore, clinical samples of GBM tissue presented overexpression of LAMP2, which correlated with advanced glioma grade and poor overall survival. In conclusion, we identified a novel role of CMA in directly regulating GSCs activity via multiple pathways at the proteome and transcriptome levels. Significance: A receptor of chaperone-mediated autophagy regulates glioblastoma stem cells and may serve as a potential biomarker for advanced tumor grade and poor survival in this disease.
5

Lescat, Laury, Vincent Véron, Brigitte Mourot, Sandrine Péron, Nathalie Chenais, Karine Dias, Natàlia Riera-Heredia, et al. "Chaperone-Mediated Autophagy in the Light of Evolution: Insight from Fish." Molecular Biology and Evolution 37, no. 10 (May 21, 2020): 2887–99. http://dx.doi.org/10.1093/molbev/msaa127.

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Abstract Chaperone-mediated autophagy (CMA) is a major pathway of lysosomal proteolysis recognized as a key player of the control of numerous cellular functions, and whose defects have been associated with several human pathologies. To date, this cellular function is presumed to be restricted to mammals and birds, due to the absence of an identifiable lysosome-associated membrane protein 2A (LAMP2A), a limiting and essential protein for CMA, in nontetrapod species. However, the recent identification of expressed sequences displaying high homology with mammalian LAMP2A in several fish species challenges that view and suggests that CMA likely appeared earlier during evolution than initially thought. In the present study, we provide a comprehensive picture of the evolutionary history of the LAMP2 gene in vertebrates and demonstrate that LAMP2 indeed appeared at the root of the vertebrate lineage. Using a fibroblast cell line from medaka fish (Oryzias latipes), we further show that the splice variant lamp2a controls, upon long-term starvation, the lysosomal accumulation of a fluorescent reporter commonly used to track CMA in mammalian cells. Finally, to address the physiological role of Lamp2a in fish, we generated knockout medaka for that specific splice variant, and found that these deficient fish exhibit severe alterations in carbohydrate and fat metabolisms, in consistency with existing data in mice deficient for CMA in liver. Altogether, our data provide the first evidence for a CMA-like pathway in fish and bring new perspectives on the use of complementary genetic models, such as zebrafish or medaka, for studying CMA in an evolutionary perspective.
6

Tang, Wanjun, Karrie Mei Yee Kiang, and Gilberto Ka Kit Leung. "Abstract A011: Enhancing chaperone-mediated autophagy to impede glioblastoma growth." Molecular Cancer Therapeutics 23, no. 6_Supplement (June 10, 2024): A011. http://dx.doi.org/10.1158/1538-8514.synthleth24-a011.

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Abstract Chaperone-mediated autophagy (CMA) is a selective form of autophagy that targets proteins with the KFERQ-like motif for lysosomal degradation. This process involves the binding of substrate proteins to the chaperone HSC70 and their subsequent transport to the receptor LAMP2A on the lysosome membrane for degradation. Previous studies have shown a significant increase in the expression level of LAMP2A and the CMA activity in glioblastoma (GBM). However, the comprehensive role of CMA in GBM pathogenesis remains unclear. In this study, we observed a progressive elevation in LAMP2A expression with increasing GBM cell confluence. Notably, inhibition of CMA through LAMP2A knockdown markedly promoted GBM growth in orthotopic mouse models, accompanied by upregulation of positive cell cycle regulators. Conversely, overexpression of LAMP2A delayed GBM cell growth and increased sub-G1 cell percentages. Treatment with CMA activators induced cell death in GBM cells while preserving normal astrocyte viability at equivalent concentrations. Our results suggest that the upregulated expression of LAMP2A in GBM cells may be influenced by the tumor microenvironment rather than being a primary driver of GBM aggressiveness. Furthermore, activating CMA could serve as a potential therapeutic strategy to inhibit GBM growth while safeguarding normal cell viability. Citation Format: Wanjun Tang, Karrie Mei Yee Kiang, Gilberto Ka Kit Leung. Enhancing chaperone-mediated autophagy to impede glioblastoma growth [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Expanding and Translating Cancer Synthetic Vulnerabilities; 2024 Jun 10-13; Montreal, Quebec, Canada. Philadelphia (PA): AACR; Mol Cancer Ther 2024;23(6 Suppl):Abstract nr A011.
7

Losmanová, Tereza, Félice A. Janser, Magali Humbert, Igor Tokarchuk, Anna M. Schläfli, Christina Neppl, Ralph A. Schmid, Mario P. Tschan, Rupert Langer, and Sabina Berezowska. "Chaperone-Mediated Autophagy Markers LAMP2A and HSC70 Are Independent Adverse Prognostic Markers in Primary Resected Squamous Cell Carcinomas of the Lung." Oxidative Medicine and Cellular Longevity 2020 (September 22, 2020): 1–12. http://dx.doi.org/10.1155/2020/8506572.

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LAMP2A and HSC70 are crucial players in chaperone-mediated autophagy (CMA), a targeted, lysosome-dependent protein degradation pathway. Elevated LAMP2A levels, indicative of increased CMA activity, are observed in several malignancies, and CMA downregulation may be exploited therapeutically. We evaluated the impact of LAMP2A and HSC70 in pulmonary squamous cell carcinomas (pSQCC). Antibodies were validated by knockdown and overexpression experiments using three different cell lines. Expression levels in tissue were analyzed by immunohistochemistry in a cohort of 336 consecutive pSQCC using tissue microarrays. There was no significant correlation between the two markers among each other and no association with pathological parameters (TNM categories, grading). However, both high LAMP2A and HSC70 expression were associated with worse outcome, including overall survival (OS; p=0.012 and p=0.001) and disease free survival (DFS; p=0.049 and p=0.036). In multivariate analysis, both markers and a combination of them were independent adverse prognostic factors for OS (LAMP2Ahigh: HR=2.059; p<0.001; HSC70high: HR=1.987; p<0.001; LAMP2Ahigh/HSC70high: HR=1.529; p<0.001) and DFS (LAMP2Ahigh: HR=1.709; p=0.004; HSC70high: HR=1.484; p=0.027; LAMP2Ahigh/HSC70high: HR=1.342, p<0.001). The negative prognostic impact of high LAMP2A and HSC70 and their variable expression in pSQCC may justify the use of these proteins as potential biomarkers for future CMA-inhibiting therapies.
8

Magnaeva, Alina S., Tat'yana I. Baranich, Dmitry N. Voronkov, Anna A. Gofman, Tat'yana S. Gulevskaya, Valeriya V. Glinkina, and Vladimir S. Sukhorukov. "IMMUNOHISTOCHEMICAL EVALUATION OF CHAPERONE-INDUCED AUTOPHAGY IN VARIOUS PARTS OF THE HUMAN BRAIN DURING AGING." Morphological newsletter 31, no. 1 (January 30, 2023): 27–33. http://dx.doi.org/10.20340/mv-mn.2023.31(1).724.

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Supporting of the functional pool of neurons during age-related brain involution is closely related to chaperone-mediated autophagy, the main function of which is to provide cellular proteostasis and utilize neurotoxic proteins. Impairment of this type of autophagy underlies the pathogenesis of many age-associated neurodegenerative diseases. In this aspect, it seems relevant to study the relationship between key markers of chaperone-mediated autophagy and their relative contribution to aging and neurodegeneration in order to determine pharmaceutical targets. The aim of the study was to determine the levels of expression of markers of chaperone-mediated autophagy - heat shock protein 70 (HSP70) and lysosome-associated membrane protein type 2A (LAMP2) in neurons of various areas of the human brain during aging. The study was performed on autopsy material of patients whose causes of death were not associated with neurological diseases. Preparations of the cortex of the precentral gyrus, striatum and hippocampus were studied in the group of cases of young age (35-45 years, n=5) and senile age (>75 years, n=10). Immunohistochemical staining was performed using primary antibodies to HSP70 and LAMP2. Optical density was assessed in the perikaryon of 150 large neurons in layer V of the cortex of the precentral gyrus, striatum, and pyramidal layer of the hippocampus. The results of the study demonstrated an increase in the expression of both HSP70 and LAMP2A in brain preparations from cases of senile age. The higher values of the optical density of HSP70 compared to LAMP2A in brain preparations from senile cases were probably due to dissociation between the stage of substrate binding and the stage of its translocation. It can be assumed that the binding of nonfunctioning proteins to HSP70 and cochaperones occurs faster than their movement into the lumen of lysosomes via LAMP2A. The results obtained suggest that the increasing of levels of markers of chaperone-mediated autophagy may be of a compensatory-adaptive nature and ensure the survival of neurons under conditions of chronic stress during physiological aging.
9

Kim, Jin-Wook, Feriel Mahiddine, and Geon Kim. "Leptin Modulates the Metastasis of Canine Inflammatory Mammary Adenocarcinoma Cells through Downregulation of Lysosomal Protective Protein Cathepsin A (CTSA)." International Journal of Molecular Sciences 21, no. 23 (November 25, 2020): 8963. http://dx.doi.org/10.3390/ijms21238963.

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Canine malignant mammary gland tumors present with a poor prognosis due to metastasis to other organs, such as lung and lymph node metastases. Unlike in human studies where obesity has been shown to increase the risk of breast cancer, this has not been well studied in veterinary science. In our preliminary study, we discovered that leptin downregulated cathepsin A, which is responsible for lysosomal-associated membrane protein 2a (LAMP2a) degradation. LAMP2a is a rate-limiting factor in chaperone-mediated autophagy and is highly active in malignant cancers. Therefore, in this study, alterations in metastatic capacity through cathepsin A by leptin, which are secreted at high levels in the blood of obese patients, were investigated. We used a canine inflammatory mammary gland adenocarcinoma (CHMp) cell line cultured with RPMI-1640 and 10% fetal bovine serum. The samples were then subjected to real-time polymerase chain reaction, Western blot, immunocytochemistry, and lysosome isolation to investigate and visualize the metastasis and chaperone-mediated autophagy-related proteins. Results showed that leptin downregulated cathepsin A expression at both transcript and protein levels, whereas LAMP2a, the rate-limiting factor of chaperone-mediated autophagy, was upregulated by inhibition of LAMP2a degradation. Furthermore, leptin promoted LAMP2a multimerization through the lysosomal mTORC2 (mTOR complex 2)/PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1)/AKT1 (Serine/threonine-protein kinase 1) pathway. These findings suggest that targeting leptin receptors can alleviate mammary gland cancer cell metastasis in dogs.
10

Sahu, Ranjit, Satwinder Singh, and Anne Davidson. "Statins induce microautophagy in RAW 264.7 cell line. (APP3P.107)." Journal of Immunology 192, no. 1_Supplement (May 1, 2014): 111.8. http://dx.doi.org/10.4049/jimmunol.192.supp.111.8.

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Abstract Autophagy is process by which cells recycle cellular components to overcome starvation. This process is constitutive in antigen presenting cells such as dendritic cells and macrophages. Among the various autophagy pathways, microautophagy (Mi) has a unique significance as it is implicated in the biogenesis of exosomes which are relevant to the design of therapeutics. Statins have been shown to up regulate macroautophagy and alleviate the effect of LPS apart from inhibiting cholesterol synthesis. However their role in microautophagy has not been reported. In the present study we investigated the effect of statins on microautophagy using the RAW264.7 cell line as a model system. Treatment with statins resulted in an up regulation of the genes for MA as well as Mi as determined by qPCR. Further studies showed that the levels of proteins TSG101 and VPS4 were increased during statin treatment. Pretreatment with statins led to a lowering of LAMP2A levels and decreased LAMP2A on the late endosomes, mimicking a LAMP2A knock down. Pretreatment with statins not only lowered the transcription of iNOS and TNF-alpha genes but also prevented the effect of LPS by decreasing initial levels of LAMP2A and increasing levels of TSG101. We propose that Mi can be a surrogate for Chaperone mediated autophagy (CMA) under conditions where the components of CMA are rendered non-functional due to disruption in normal cellular metabolism.
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Journal articles Dissertations / Theses Books

Dissertations / Theses on the topic "LAMP2a":

1

Lescat, Laury. "Caractérisation et étude du rôle de lamp2a chez les poissons." Thesis, Pau, 2019. http://www.theses.fr/2019PAUU3014.

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L’Autophagie médiée par les protéines chaperonnes (ou CMA pour Chaperone-Mediated Autophagy) est une voie majeure du catabolisme lysosomal considérée aujourd’hui comme un acteur central de contrôle de nombreuses fonctions cellulaires, et dont les défauts sont associés à plusieurs pathologies humaines, dont des maladies neurodégénératives, des cancers et des troubles du système immunitaire. Selon l’idée actuellement admise, cette fonction cellulaire n’existerait que chez les mammifères ou les oiseaux, qui seraient les seuls à exprimer la protéine LAMP2A, une protéine nécessaire au fonctionnement de la CMA. Or, récemment, nous avons pu mettre en évidence l’existence de séquences exprimées présentant une forte homologie avec LAMP2A de mammifères chez plusieurs espèces de poissons, remettant ainsi en question ce point de vue et suggérant que la CMA soit apparue beaucoup plus tôt au cours de l'évolution qu'on ne l'avait initialement cru. Dans cette thèse, nous retraçons l’histoire évolutive du gène LAMP2 chez les vertébrés. Nous démontrons que ce gène est apparu après la seconde duplication complète du génome survenue chez l'ancêtre commun des vertébrés il y a environ 500 millions d'années. En outre, en adaptant une méthode récemment décrite pour mesurer l’activité de la CMA dans des cellules de mammifères à une lignée de fibroblastes de medaka (Oryzias latipes), nous apportons la preuve de l’existence de cette fonction cellulaire chez cette espèce de poisson. Enfin, afin de caractériser le rôle physiologique de cette fonction chez les poissons, nous avons procédé à l’invalidation par crispR-cas9 de lamp2a chez le medaka. Les poissons générés présentaient de sévères perturbations du métabolisme intermédiaire, comme précédemment décrit chez des souris dont LAMP2A a été invalidée dans le foie. Dans l’ensemble, ces résultats démontrent clairement, et pour la toute première fois, qu’il existe bien une activité CMA fonctionnelle chez les poissons, et apportent ainsi de nouvelles perspectives dans ce domaine de recherche, notamment en autorisant l'utilisation de modèles génétiques complémentaires, tels que le poisson zèbre ou le medaka, pour faire avancer nos connaissances sur les mécanismes régissant cette fonction cellulaire
Chaperone-Mediated Autophagy (CMA) is a major pathway of lysosomal proteolysis recognized as a key player in the control of numerous cellular functions, and whose defects have been associated to several human pathologies, including neurodegenerative diseases, cancers and immune disorders. To date, this cellular function was presumed to be restricted to mammals and birds, due to the absence of an identifiable lysosome-associated membrane protein 2A (LAMP2A), a limiting and essential protein for CMA, in non-tetrapod species. However, we recently identified the existence of expressed sequences displaying high homology with the mammalian LAMP2A in several fish species, challenging that view and suggesting that CMA appeared much earlier during evolution than initially thought. In the present thesis, we first present new evidences about the evolutionary history of the gene LAMP2 in vertebrates. We demonstrate that LAMP2 appeared after the second whole genome duplication that occurred at the root of the vertebrate lineage approximately 500 million years ago. By using a fluorescent reporter previously used to track CMA in mammalian cells, we then revealed the existence of a CMA-like pathway in a fibroblast cell line of the fish medaka (Oryzias latipes). Finally, to address the physiological role of Lamp2a in fish, we generated, medaka knockout for the splice variant lamp2a, and found severe alterations in the intermediary metabolism, as previously demonstrated in mice deficient for CMA in liver. Altogether, our data provide the first evidence for a CMA-like pathway in fish and bring new perspectives on the use of complementary genetic models, such as zebrafish or medaka, for studying CMA in an evolutionary perspective
2

Ramos, dos Santos Ligia. "Évaluation de la contribution de la fonction du facteur de transcription de la parkine dans la régulation de l'alpha-synucléine dans la maladie de Parkinson." Electronic Thesis or Diss., Université Côte d'Azur, 2023. http://theses.univ-cotedazur.fr/2023COAZ6012.

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La maladie de Parkinson (MP) est une affection neurodégénérative liée à l'âge qui touche plus de 8 millions de personnes dans le monde. Elle peut être d'origine génétique ou sporadique. Elle se caractérise au niveau histopathologique par la présence de corps de Lewy et la perte de neurones dopaminergiques au niveau de la substantia nigra par compacta. Au fil des décennies, les traitements pharmacologiques ont contribué à une amélioration de la qualité de vie des patients, mais n'ont pas permis de les guérir. Pour établir un traitement curatif potentiel, il est important de comprendre les mécanismes moléculaires qui sous-tendent le dysfonctionnement des protéines clés liées à la MP, comme l'α-synucléine (α-syn) et la parkine. L'objectif principal de cette étude était donc d'examiner les mécanismes directs ou indirects de régulation de l'α-syn par la parkine dans la MP. Les objectifs spécifiques étaient les suivants : étudier l'impact de la parkine sur les régulations transcriptionnelles des gènes codant pour l'α-syn (SNCA) et la glucocérébrosidase 1 (GBA1) ; inspecter le rôle de la parkine sur le contrôle de la CMA («chaperonne-mediated autophagy»); évaluer la contribution de la GBA1 dans le contrôle de l'α-syn et de l'CMA par la parkine ; établir la capacité de la parkine à réguler l'α-syn, la GBA1 et la CMA in vivo et dans les cerveaux humains. Nos résultats montrent que la parkine contrôle la transcription des gènes SNCA et GBA1. Nous montrons que cette régulation transcriptionnelle est médiée par l'interaction de la parkine avec les promoteurs des gènes SNCA et GBA1. Nous démontrons que la parkine contrôle l'CMA par l'intermédiaire de GBA1. Des études in vivo basées sur le traitement au paraquat de souris témoins et knock-out pour la parkine corroborent l'interaction moléculaire entre la parkine et l'α-syn dans la MP sporadique. Enfin, l'analyse des nécropsies humaines indique une corrélation entre les niveaux de parkine et d'α-syn phosphorylée, ce qui renforce les données obtenues in vitro, ex-vivo et in vivo.En conclusion, nos résultats corroborent non seulement l'idée que la parkine est une protéine neuroprotectrice, mais délimitent également une nouvelle voie moléculaire dans laquelle le contrôle transcriptionnel de l'α-synucléine et du GBA1 par la parkine a un impact sur la réponse à l'AMC. En outre, nos données ont fourni des informations importantes sur la régulation d'un acteur pathologique clé de la maladie de Parkinson et, par conséquent, peuvent conduire à une meilleure gestion thérapeutique et diagnostique de la maladie à l'avenir
Parkinson's disease (PD) is an age-related neurodegenerative disorder that affects more than 8 million people worldwide. It may be of a genetic or sporadic origin. It is characterized at histopathological level by the presence of Lewy bodies and the loss of dopaminergic neurons at substantia nigra par compacta. Over decades, pharmacological treatments and physical therapies have contributed to an amelioration of health quality of life of patients, but did not allow the cure of the patients. To stablish a potential curative treatment, it is important to understand the molecular mechanisms underlying the dysfunction of key PD causative proteins, as α-synuclein (α -syn) and parkin. Thus, the main objective of this study was to examine the direct or indirect mechanisms of regulation of α-syn by parkin in PD. The specific objectives were to investigate the impact of parkin on the transcriptional regulations of the genes coding for α-syn (SNCA) and glucocerebrosidase 1 (GBA1); to inspect CMA control by parkin; evaluate the contribution of GBA1 in parkin-mediated α-syn and CMA control; to establish parkin capacity of regulating α-syn, GBA1 and CMA in vivo and human brains. Our results show that parkin controls the transcription of SNCA and GBA1 genes. We show that this transcriptional regulation is mediated by the interaction of parkin with the SNCA and GBA1 promoters. We demonstrate that parkin controls CMA via GBA1. In vivo studies based on paraquat treatment of control and parkin knockout mice, corroborate the interplay between parkin and α-syn in sporadic PD. Finally, the analysis of human PD necropsies indicates a correlation between parkin and phosphorylated α-syn levels reinforcing the data obtained in vitro, ex-vivo and in vivo. In conclusion, our results not only corroborate the notion that parkin is a neuroprotective protein, but also delineate a new molecular pathway in which parkin transcriptional control of α-synuclein and GBA1 impacts CMA response. Moreover, our data provided important insights on the regulation of a key pathological player in PD and by consequence may lead to a better therapeutic and diagnostic management of the disease in the future
3

Engquist, Elin. "BOEL-lampan : Utveckling av en lampa för visualisering av elförbrukning i hemmet." Thesis, Mälardalen University, School of Innovation, Design and Engineering, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-7442.

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Den här rapporten behandlar det examensarbete som har utförts på avancerad nivå av Elin Engquist på utbildningen Innovation och produktdesign vid Mälardalens högtskola. Arbetet har utförts under våren 2009 i samarbete med Interactive Institute's avdelning Energy Design i Eskilstuna. Examensarbetet har avsett produktutveckling av en artefakt för visualisering av elförbrukning i hemmet med anledning av den ständigt ökande konsumtionen av hushållsel. Artefakten har under arbetets gång döpts till BOEL-lampan och framtagandet av denna har skett som en del av forskningsprojektet Visuale som utförts vid Interactive Institue. Forskningsprojektet syftade till att göra konsumeter mer medvetna om sin elförbrukning och som en del av projektet behövde en forskningsstudie genomföras. I forskningsstudien var målet att ta reda på om konsumenters beteende gällande elanvändning kunde påverkas med hjälp av ett tävlingsmoment grannar emellan där en webbsida fanns tillgänglig med en personlig profil och en artefakt (BOEL-lampan) som placerad i fönstret kunde visa olika interaktiva färger. Färgerna som implementerades i tävlingen var rött för tappad placering, gult för samma placering och grönt för högre placering.  Några av de krav som ställdes på BOEL-lampan var att den skulle kunna visa de olika färgerna och att den skulle kunna uppdateras en gång om dagen då tävlingsmomentet byggde på en procentuell ökning eller minskning av föregående dags elförbrukning, vilket avgjorde tävlingsplaceringen för deltagarna nästkommande dag. Vidare behövde ändringen av färg på BOEL-lampan kunna ske trådlöst för att inget intrång i hushållen skulle behöva göras under tävlingens gång. Det fanns även krav på att lampan skulle vara så energieffektiv som möjligt, ha så liten miljöpåverkan som möjligt, samt att priset inte skulle vara allt för högt. En helhetsbild innehållande alla dessa delar behövdes och där kommersialisering i framtiden fanns i åtanke.

Utvecklingsarbetet med lampan delades in fyra olika delar: trådlös kommunikation, ljuskälla, material samt färg och form. Konceptframtagning skedde för de olika delarna där möjliga alternativ utvecklades och utvärderades. Slutligen skedde ett konceptval inom respektive del och utifrån detta togs lämpligaste tillverkningsmetod fram. För den trådlösa kommunikationen valdes ZigBee/XBee teknik då detta var det bästa alternativet med avseende på pris, energieffektivitet samt möjlighet till uppstartande av nätverk. Till ljuskälla valdes LED-dioder av RGB variant där varje BOEL-lampa innehöll tre RGB-dioder. Materialet som användes för kroppen på lampan var silikon med anledning av ljusspridning och formbarhet, och för toppen på lampan användes plast av formbarhetsskäl. Formen på BOEL-lampan var inspirerad av en droppe och gav lampan en avlång topp och ett runt klot till underdel. Färgen på toppen, och likaså sladden, vart svart då detta var en diskret färg som gick bra ihop med silikonets genomskinliga men något gulaktiga ton.

BOEL-lampan tillverkades manuellt i sex exemplar för forskningsstudien i verkstaden på Mälardalens högskola. Lamporna fungerade på så sätt som tänkt och kunde efter tillverkning sättas upp i testhushållen som medverkade i forskningsstudien. Då lamporna tillverkats upptäcktes vissa aspekter som skulle behöva ändras för framtiden. Silikonet var tungt och färgen på lampan syntes nästan inte alls dagtid, så valet av material och ljuskälla behöver ses över. Vidare är tillverkningsmetoden inte anpassad för serietillverkning, så nytt underlag för detta behöver tas fram. Det finns även utrymme för kostnadsminskningar och en mer noggrann analys av ingående delar behövs.

4

Johansson, Emma. "Tre lampor och ett ben : produktserie för Gotlands Lampfabrik." Thesis, Gotland University, School of the Humanities and Social Science, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:hgo:diva-413.

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Gotlands Lampfabrik är en nystartad verksamhet (2009) som drivs av Jonas Kling och Jane Odell centralt på Gotland. De producerar stilrena lampskärmar i ett ljust polymermaterial med papp på båda sidorna.

Produktionen är liten och görs för hand på plats i den egna fabriken i Väskinde.

Sortimentet består av 33 lampskärmar och i nuläget producerar inte Gotlands Lampfabrik sina egna lampben eller upphängningar utan köper in dem från fristående parter vilket medför att sortimentet kan uppfattas som spretigt och osammanhängande. Dagens sortiment saknar även vägglampor och ljuskällorna som används är glödlampor som från och med 2009 håller på att urfasas från den Europeiska marknaden efter ett beslut från EU.

Möjligheten att kunna välja lampor från samma serie till sitt hem är både efterfrågat samt kan ge en känsla av sammanhållen och genomtänkt interiör. Genom att göra en produktserie med tillhörande upphängning samt lampben skulle verksamheten kunna profilera sig bättre och få en mer tydlig produktidentitet.

I ett nära samarbete med Jonas på Gotlands Lampfabrik kom vi gemensamt överens om att skapa en ny produktserie med tydligt släktskap till varandra. I produktserien skulle en taklampa, en golvlampa samt en bordslampa ingå men efter undersökningar tog vi beslutet att byta ut bordslampan till en vägglampa då Gotlands Lampfabrik saknade denna armatur helt. För att tydligare visa på produktseriens tillhörighet till varandra valde jag även att utveckla ett ben till golvlampan. Då vi arbetat bort bordslampan och den möjliga tillhörande lampbenet fann jag det omöjligt att skapa en upphängning för vägglampan som synligt tillhörde resten av produktserien.

Projektet började med förstudier då jag gjorde en enkätundersökning, marknadsundersökning, trendspaning samt samlade in fakta som sedan legat till grund för arbetet. Efter sammanställning av fakta togs skisser och enklare modeller fram i papper för att presentera mina konceptförslag. Tillsammans med Jonas på Gotlands Lampfabrik diskuterade vi fram ett favoritförslag som resulterade i ytterligare ett koncept som vi båda kände oss nöjda med och sedan producerade jag serien i fabriken. Projektet resulterade i tre lampskärmar, en tak-, en vägg- och en golvlampa med tillhörande lampben för golvlampan. Lampbenet består av ett modulsystem med utbytbara delar där kunden själv får välja vilken form och material som just deras lampben ska ha. Samtliga lampor är försedda med tvinnad textilsladd för en mer exklusiv känsla och är framtagna i det vita plastlaminatet som Gotlands Lampfabrik redan använder. De har även en liten märkning på sladden som visar att den kommer från Gotlands Lampfabrik. Ljuskällan med överlägsna fördelar för projektet blev Philips MasterLED 7W vilket ger en behaglig belysning.


Gotlands Lampfabrik is a new business (2009) run by Jonas Kling and Jane Odell on Gotland in Sweden. They produce stylish lampshades in a bright polymer material with paper on both sides. Their production is small and made by hand in their own workshop in Väskinde with a current collection consisting of 33 lamps.

Gotlands Lampfabrik currently does not produce their own lamp fixtures or suspensions but instead buys them from other companies, as a result making the collection seem unclear and incoherent.

The current collection also lacks wall lamps and the light sources are outdated light-bulbs that are slowly being taken off the market after a decision from the EU.

The possibility to select well designed lamps from a series for your home are both in high-demand and can provide a sense of coherency and well though-out interior design for the consumer.

By making a new, carefully put together product series and lamp-foot, branding for Gotlands Lampfabrik would be easier and help their lamps have a more clear product identity.

In a close collaboration with Jonas we both agreed that I would create a new product series for Gotlands Lampfabrik with a distinct design relationship within the new series. The product series would include a hanging lamp, a floor lamp and a table lamp but after some discussion and studies we took the decision to replace the table lamp with a wall lamp since Gotlands Lampfabrik lacked this fixture in their collection.

The project started with a pilot study where I did market research, a survey and investigated trends within lighting and lamps. After I compiled these facts I made sketches and models and presented them for Gotlands Lampfabrik. Together with Jonas we discussed and picked our favourite concepts which was developed further. The final concept we both agreed on was produced by me in their workshop. The project resulted in three lamps and a modular system for the floor lamp fixture where consumers can select the shape and materials by themselves. All lamps are equipped with a textile cord for a more exclusive feel and are designed in the white plastic laminate that Gotlands Lampfabrik already use in production. The lamps also have a small label on the cord which shows that it comes from Gotlands Lampfabrik. The light source that had most benefits, and was well suited for this project was Philips MasterLED 7W.

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Upsal, Isaac. "Global Polarization of the Lamba/Anti-Lambda System in the STAR BES." The Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu152345697657853.

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Hermanns, Marcus Heinrich. "Licht und Lampen im westgriechischen Alltag Beleuchtungsgerät des 6.-3. Jhs. v. Chr. in Selinunt /." Rahden/Westf. : Leidorf, 2004. http://catalog.hathitrust.org/api/volumes/oclc/60520545.html.

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Forssman, Niklas. "Monte Carlo simulation study of the e+e- →Lambda Lamba-Bar reaction with the BESIII experiment." Thesis, Uppsala universitet, Kärnfysik, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-297851.

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Studying the reactions where electrons and positrons collide and annihilate so that hadrons can be formed from their energy is an excellent tool when we try to improve our understanding of the standard model. Hadrons are composite quark systems held together by the strong force. By doing precise measurements of the, so called, cross section of the hadron production that was generated during the annihilation one can obtain information about the electromagnetic form factors, GE and GM, which describe the inner electromagnetic structure of hadrons. This will give us a better understanding of the strong force and the standard model.During my bachelor degree project I have been using data from the BESIII detector located at the Beijing Electron-Positron Collider (BEPC-II) in China. Uppsala university has several scientists working with the BESIII experiment. My task was to do a quality assurance of previous results for the reaction e+e-→ Lambda Lambda-Bar at a center of mass energy of 2.396 GeV. During a major part of the project I have been working with Monte Carlo data. Generating the reactions was done with two generators, ConExc and PHSP. The generators was used for different means. I have analyzed the simulated data to find a method of filtering out the background noise in order to extract a clean signal. Dr Cui Li at the hadron physics group at Uppsala university have worked with several selection criteria to extract these signals. The total efficiency of Cui Li's analysis was 14%. For my analysis I also obtained total efficiency of 14%. This gave me confidence that my analysis have been implemented in a correct fashion and that my analysis now can be transferred over to real data. It is also reassuring for Cui Li and the rest of the group that her analysis has been verified by and independently implemented selection algorithm.
Att studera vad som händer vid reaktioner där elektroner och positroner kolliderar och annihilerar så att hadroner kan bildas ur energin kan vara till stor hjälp när vi vill förstå standardmodellen och dess krafter, i synnerhet den starka kraften, som kan studeras i sådana reaktioner. Genom att utföra precisa mätningar av tvärsnitt för hadronproduktion får man fram de elektromagnetiska formfaktorerna GE och GM som beskriver hadronernas inre struktur. Hadroner är sammansatta system av kvarkar och den starka kraften binder dessa kvarkar.\\Under mitt examensarbete har jag använt mig av data från detektorn BESIII som finns vid BEPC-II (Beijing Electron-Positron Collider) i Kina. Uppsala universitet har flera forskare som jobbar med BESIII experimentet. Målet var att kvalitetssäkra den tidigare analys som gjorts för reaktionen e+e- → Lambda Lambda-Bar vid 2.396 GeV. Jag började med att göra Monte Carlo-simuleringar. Reaktionerna har genererats med två olika generatorer, ConExc och PHSP. Dessa generatorer har använts till olika ändamål. De genererade partiklarnas färd genom detektorn har sedan simulerats. Då bildas data av samma typ som dem man får från experiment. Jag har analyserat dessa simulerade data för att hitta en metod som kan filtrera bort bakgrundsstörningar samtidigt som intressanta data sparas. Kriterier utarbetade av Dr. Cui Li har använts för att skapa denna metod. Min algortim gav en total effektivitet på 14%, vilket stämmer bra med den tidigare algoritmen som Cui Li skapade, även där var effektiviteten 14%. Detta ger förtroende för min algortim och den stärker även Cui Lis resultat.
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Puliyambalath, Naushad Pasha. "Lambda designs for lambda less than 60." The Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1253556251.

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Weisz, Iván. "Lambda-Designs with Small Lambda are Type-1 /." The Ohio State University, 1995. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487929230740028.

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Burkhardt, Mario. "Cykel Lampa : Processbeskrivning för mitt kandidatexamensarbete." Thesis, Linnéuniversitetet, Institutionen för design (DE), 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-36687.

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Med examensarbetet vill jag undersöka med ett holistiskt synsätt på hela livscykeln av ljuset vilka krav en modern användare har av ett cykelljus och hur dessa anspråk kan åstadkommas.
 För att skapa ett cykelljus som fungerar för en modern cykelanvändare utifrån deras krav och behov samt tar hänsyn till alla stakeholders förväntningar. Först gör jag en grundlig research på historiska cykelbelysningar och vad som finns idag, jag kommer att skapa ett ITK och göra Trendanalyser samt Brain- storming, Scenario och Personas. Sedan kommer jag att laborera med Ljust- ester innan jag går över till formgivningen där jag kommer att använda mig av 3D Modeller. Under hela processen kommer jag att skriva och uppdatera min Workbook.
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Books on the topic "LAMP2a":

1

Švantner, Ján. Lampa. Bratislava: Smena, 1986.

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Kužel, Dušan. Lampa. Bratislava: Slovenský spisovatel̕, 1991.

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Švantner, Ján. Lampa. Bratislava: Smena, 1986.

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Kužel, Dušan. Lampa. Bratislava: Slovenský spisovatel̓, 1991.

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Smiljanić, Dobroslav. Uronjena lampa. Beograd: Prosveta, 1998.

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Serebrova, Galina. Malinovai͡a lampa. Sankt-Peterburg: Gelikon pli͡us, 2009.

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Libedinskai︠a︡, L. Zelena︠i︡a lampa. Moskva: AST, 2012.

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Łsova, Dar £i Ła Dont. Lampa razyskivaet Alladina. Moskva: E KSMO, 2007.

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Slepakova, Nonna. Lampa: Kniga stikhov. Sankt Peterburg: Sovetskiĭ pisatelʹ, 1992.

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Ti͡urin, Aleksandr. Volshebnai͡a lampa genseka. Sankt-Peterburg: Lanʹ, 1995.

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Book chapters on the topic "LAMP2a":

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Kalamida, D., A. Giatromanolaki, and M. I. Koukourakis. "Characterization of the “Autophagic Flux” in Prostate Cancer Tissue Biopsies by LC3A/LAMP2a Immunofluorescence and Confocal Microscopy." In Methods in Molecular Biology, 555–60. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-8873-0_36.

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Losmanová, Tereza, Mario P. Tschan, José A. Galván, and Sabina Berezowska. "Immunohistochemical Detection of the Chaperone-Mediated Autophagy Markers LAMP2A and HSPA8 in Formalin-Fixed and Paraffin-Embedded Tissues." In Methods in Molecular Biology, 141–47. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2675-7_11.

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Gooch, Jan W. "Lambda Zero ($$\lambda $$o)." In Encyclopedic Dictionary of Polymers, 418. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_6750.

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Gooch, Jan W. "Lamina." In Encyclopedic Dictionary of Polymers, 418. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_6757.

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Baldi, Mario, and Yoram Ofek. "Lambda and Sub-Lambda Switching." In Handbook of Computer Networks, 767–82. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2011. http://dx.doi.org/10.1002/9781118256053.ch48.

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Miranda, Guilherme Henrique Santos, Alexsandro Oliveira Alexandrino, Carla Negri Lintzmayer, and Zanoni Dias. "Sorting $$\lambda $$-Permutations by $$\lambda $$-Operations." In Advances in Bioinformatics and Computational Biology, 1–13. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-030-01722-4_1.

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Horton, Ivor. "Lambda Expressions." In Beginning C++, 271–85. Berkeley, CA: Apress, 2014. http://dx.doi.org/10.1007/978-1-4842-0007-0_9.

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Sarcar, Vaskaran. "Lambda Expressions." In Getting Started with Advanced C#, 71–99. Berkeley, CA: Apress, 2020. http://dx.doi.org/10.1007/978-1-4842-5934-4_3.

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Sharan, Kishori. "Lambda Expressions." In Java Language Features, 169–222. Berkeley, CA: Apress, 2018. http://dx.doi.org/10.1007/978-1-4842-3348-1_5.

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Olsson, Mikael. "Lambda Expressions." In Java Quick Syntax Reference, 105–8. Berkeley, CA: Apress, 2018. http://dx.doi.org/10.1007/978-1-4842-3441-9_23.

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Conference papers on the topic "LAMP2a":

1

Lall, Pradeep, Peter Sakalaukus, and Lynn Davis. "An Investigation of Catastrophic Failure in Solid-State Lamps Exposed to Harsh Environment Operational Conditions." In ASME 2015 International Technical Conference and Exhibition on Packaging and Integration of Electronic and Photonic Microsystems collocated with the ASME 2015 13th International Conference on Nanochannels, Microchannels, and Minichannels. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/ipack2015-48257.

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Today’s lighting technology is steadily becoming more energy efficient and less toxic to the environment since the passing of the Energy Independence and Security Act of 2007 (EISA) [1]. EISA has mandated a higher energy efficiency standard for lighting products and the phase out of the common incandescent lamp. This has led lighting manufacturers to pursue solid-state lighting (SSL) technologies for consumer lighting applications. However, two major roadblocks are hindering the transition process to SSL lamps: cost and quality. In order to cut cost, manufactures are moving towards cheaper packaging materials and a variety of package architecture construction techniques which may potentially erode the quality of the lamp and reduce its survivability in everyday applications. Typically, SSL lamps are given product lifetimes of over twenty years based off of the IES TM-21-11 lighting standard which does not include moisture effects or large operational temperatures [2]. A group of recently released off-the-shelf lamps have undergone a steady-state temperature humidity bias life test of 85°C/85%RH (85/85) to investigate the reliability in harsh environment applications. The lack of accelerated test methods for lamps to assess reliability prior to introduction into the marketplace does not exist in literature. There is a need for SSL physics based models for the assessment and prediction of a lamp’s lifetime which is being spearheaded by the DOE [3]. In order to be fully accepted in the marketplace, SSL lamps must be able to perform similarly to incandescent lamps in these environments, as well as live up to the lifetime claims of manufacturers. A lamp’s package architecture must be designed with performance factors in mind, as well as address some of the known and published package related failure mechanisms, such as carbonization of the encapsulant material, delamination, encapsulant yellowing, lens cracking, and phosphor thermal quenching [4]. Each failure mechanism produces the similar failure mode of lumen degradation predominately due to two contributing factors: high junction temperature and moisture ingress. The current state-of-the-art has focused on individual areas of the lamp, such as the LED chip, substrate material, electrical driver design and thermal management techniques. [5] – [16] Looking at the lamp as a whole is a novel approach and has not been seen before in literature. This work followed the JEDEC standard JESD22-A101C of 85/85 with a one hour interval of applied voltage followed by a one hour interval of no applied voltage [17]. This test was performed continuously for each SSL lamp until it became nonoperational, i.e. did not turn on. Periodically, photometric measurements were taken following the IES LM-79-08 standard at room temperature using an integrating sphere, a spectrometer, and lighting software. The overall health of the SSL lamps was assed using the relative luminous flux (RLF), correlated color temperature (CCT) and the color difference (Δu′v′) using the Euclidean distance of the CIE 1976 color space coordinates. Finally, a Weibull analysis was completed to compare the characteristic lifetime of the SSL lamp to the actual rated lifetime. An important result from this work shows that the rated lifetime does not come close to the actual lifetime when the SSL lamps are used in a harsh humid environment which is fairly common in outdoor applications across the U.S. Also, the photometric results are presented for the entire lifetime of each SSL lamp under test.
2

Saakes, Daniel. "Big lampan lamps." In Proceeding of the seventh ACM conference. New York, New York, USA: ACM Press, 2009. http://dx.doi.org/10.1145/1640233.1640322.

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Joshy, Hanna C., Mansoor Ali K, Rameez Z, Sarath S, and Ramaswamy K. P. "A Suitability Study of Using Crushed Fluorescent Lamp Waste as Fine Aggregates." In International Web Conference in Civil Engineering for a Sustainable Planet. AIJR Publisher, 2021. http://dx.doi.org/10.21467/proceedings.112.42.

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Fluorescent lamp wastes are one of the discarded electrical devices. These lamp wastes pose serious health concerns, and require extreme care in its disposal to avoid any adverse impacts. Also, with the widespread use of energy efficient LED lamps for lighting purposes, environmental concerns related to disposal of used mercury containing fluorescent lamps have become progressively important. This paper explores the possibility of replacement of fine aggregates in mortar with different proportions of fluorescent lamp wastes in order to find the optimum percentage of replacement to get better properties. Fluorescent lamp wastes were collected and crushed to the grading of fine aggregates. Compressive strength and bulk density of mortar cubes with 0%, 25%, 50%, 75% and 100% replacement of fine aggregates with fluorescent lamp wastes were evaluated after 7 days of initial curing. The compressive strength and bulk density of mortar was found to be decreasing with increasing percentage of lamp waste added. The replacement of fine aggregate by 25% lamp waste has greater strength compared to other proportions. Bulk density was found to be lesser at higher replacement levels and can be tried in producing light weight mortar. The utilization of lamp waste in mortar/concrete is a noble attempt in reducing the negative impact of lamp wastes on the environment
4

Goodman, Douglas S. "Fiber-optic illuminators for microscopy." In OSA Annual Meeting. Washington, D.C.: Optica Publishing Group, 1987. http://dx.doi.org/10.1364/oam.1987.wg6.

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Many illumination problems in microscopy can be solved easily and economically with fiber optics. Modes of illumination not provided by the designers of a microscope can be added. A modular illumination system involving a number of microscopes and sources can be assembled. Various types of illumination can be used simultaneously, e.g., bright field and dark field, transmitted and reflected. Realignment on changing lamps is simplified, since it is merely necessary to align the lamp relative to the fiber input end. Older microscopes with small lamps can be upgraded by using a fiber bundle terminated at the lamp filament location.
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Ishizuka, Masaru, Shinji Nakagawa, and Katsuhiro Koizumi. "Practical Study of Application of Thermal Network Method to Thermal Design of Electronic Equipment: An Example for the Thermal Design of a Compact Self-Ballasted Fluorescent Lamp." In ASME 2006 International Mechanical Engineering Congress and Exposition. ASMEDC, 2006. http://dx.doi.org/10.1115/imece2006-13105.

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Thermal design is one of the most important issues in the development of compact self—ballasted fluorescent lamps as the demand for small yet powerful lamps is mounting. This paper proposes a simulation method that is based on a thermal network model for which a set of equations are developed. Some of the coefficients of the thermal network equations were determined experimentally using a simulated model lamp. The calculated temperatures are in good agreement with the measured temperatures. The work illustrates the usefulness of the proposed methodology in the design of compact self—ballasted fluorescent lamps.
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Remillard, J. T., M. A. Marinelli, T. Fohl, and D. A. O'Neil. "Diode laser illuminated automotive brake lamp using a linear fanout diffractive optical element." In Diffractive Optics and Micro-Optics. Washington, D.C.: Optica Publishing Group, 1998. http://dx.doi.org/10.1364/domo.1998.dwa.5.

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Marinelli and Remillard discussed the design and fabrication of diode laser illuminated automotive signal lamps.1 The optics are made from thin (~5 mm) plastic sheets that manipulate light using reflection and refraction from edges, surfaces, and shaped cut-outs. The resulting lamps can be well under 1 inch in thickness providing functional and styling advantages not attainable with conventional automotive lighting systems. Functionally, the sheet optic consists of a section that receives, divides, and expands the laser light (the manifold), and a section that directs it out of the lamp into the appropriate beam pattern (the kicker). The kicker is the visible part of the lamp optic, and the most efficient use of space is realized by maximizing the ratio of kicker to manifold area. Here we describe the design and fabrication of a thin, laser illuminated brake lamp that uses a diffractive optical element (DOE) at the entrance of the manifold. The DOE provides a compact way of uniformly spreading the light exiting the fiber, and allows for smaller manifolds than those discussed in the previous work.
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Usman, Asad A., and Mohammad Usman. "Determination of Geometric Distortions in Automotive Lamps Using Non-Linear Parametric Estimations." In ASME 2002 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2002. http://dx.doi.org/10.1115/detc2002/dac-34071.

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In automotive lamps, an ideal paraboloid is the reflector shape of choice when lens optics is utilized. However, geometric distortions occur among manufactured automotive lamps. This paper discusses the effects of geometric distortions on spread, packing, and gradient of reflected light from automotive lamps. Relevant legal requirements set by Federal Motor Vehicle Safety Standard on the performance of automotive lamps are also discussed. A new parametric mathematical model is developed to represent the geometry of an ideal lamp reflector. A non-linear parametric estimation problem is formulated using the Box-Kanemasu modification of the Gauss method. An application of methodology is also presented in this paper. The results show significant distortions of paraboloidal reflector with respect to the ideal design-intent reflector geometry. The numerically calculated deviations of focal point, focal length and paraboloidal axis from the ideally designed reflector necessitate improvements in the tooling and the manufacturing process for better dimensional control.
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Lall, Pradeep, Junchao Wei, and Peter Sakalaukus. "Fault-Mode Classification of Solid State Luminaires Using Bayesian Probabilistic Models." In ASME 2014 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2014. http://dx.doi.org/10.1115/imece2014-39523.

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A new method has been developed for assessment of the onset of degradation in solid state luminaires to classify failure mechanisms by using metrics beyond lumen degradation that are currently used for identification of failure. Luminous Flux output, Correlated Color Temperature Data on Philips LED Lamps has been gathered under 85°C/85%RH till lamp failure. The acquired data has been used in conjunction with Bayesian Probabilistic Models to identify luminaires with onset of degradation much prior to failure through identification of decision boundaries between lamps with accrued damage and lamps beyond the failure threshold in the feature space. In addition luminaires with different failure modes have been classified separately from healthy pristine luminaires. It is expected that, the new test technique will allow the development of failure distributions without testing till L70 life for the manifestation of failure.
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Tavkari, Devang, and Paul Davidson. "Thermal Characterization of Xenon Flash Lamp System for Automated Fiber Placement." In ASME 2024 Aerospace Structures, Structural Dynamics, and Materials Conference. American Society of Mechanical Engineers, 2024. http://dx.doi.org/10.1115/ssdm2024-121589.

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Abstract Carbon fiber Thermoplastic pre-preg tape laydown and consolidation used with Automated Fiber Placement (AFP) required high temperatures to melt the matrix. There are a few methods that can reach the desired temperature. Hot-gas torch, laser heating, and arc-flash lamp-based heating systems are the most popular among them. Among the three, arc-flash lamp-based heating provides the most versatility regarding heating range and controllability. The lamp pulses light of high radiation intensity to heat the tow and substrate material during laydown rapidly. The irradiance output by the lamp is controlled by three process parameters, namely, (1) pulse width, (2) pulsing frequency, and (3) voltage. Further control can be achieved by changing the distance between the flash lamp and the tows. Therefore, the irradiance output from the lamp can be widely varied by changing the process and geometric parameters. This study aims to characterize the irradiance output from a xenon arc-flash heating system and its control parameters. The Xenon flash lamp’s irradiance response was used to map the irradiance profile on the tow and substrate within an Automated Fiber Placement machine. The impact of the AFP control parameters like roller compaction, tow layup speed, and positioning of xenon flash lamp on the irradiance profile of the tow and substrate were also examined in this study.
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Harter, D. J., Y. B. Band, H. Samelson, and E. P. Ippen. "Stabilization and Passive Mode Locking of CW Alexandrite Lasers." In Instabilities and Dynamics of Lasers and Nonlinear Optical Systems. Washington, D.C.: Optica Publishing Group, 1985. http://dx.doi.org/10.1364/idlnos.1985.fc3.

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Alexandrite is a solid state laser material which lases in the range of 700-800 nm. Alexandrite has been lased continuously by pumping with a krypton ion laser1 and by pumping with xenon and mercury arc lamps2. When alexandrite is pumped with the krypton ion laser, the output is very stable and, when the pump source is removed, the output damps through population oscillations which can be well described by rate equations3. However, when alexandrite is pumped by the xenon or mercury arc lamps, the output is very noisy at certain pumping levels. Experimental data showing this noisy operation will be presented and sources of this noise will be discussed.

Reports on the topic "LAMP2a":

1

Matsumura, Akihiko. Spectroscopy of 28Al(Lambda), 12B(Lambda), 7He(Lambda) by the (e,e'K+) Reaction. Office of Scientific and Technical Information (OSTI), January 2009. http://dx.doi.org/10.2172/992499.

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MacKay, Waldo, Steve Peggs, Chris Saltmarsh, Todd Satogata, Fady Harfoush, Jim Holt, Leo Michelotti, et al. Lambda Manual. Office of Scientific and Technical Information (OSTI), October 1993. http://dx.doi.org/10.2172/1119409.

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Witte, Grimley, and Thorson. PR-015-12606-R01 Thermal Irradiance Effects on Ultrasonic Meter Performance at Low Flow Rates. Chantilly, Virginia: Pipeline Research Council International, Inc. (PRCI), October 2013. http://dx.doi.org/10.55274/r0010011.

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This study was focused on determining the effects of thermal radiant energy on ultrasonic meter accuracy at flow rates less than 10% of meter capacity. A straight run ultrasonic meter installation consistent with the recommendations of AGA Report Number 9 was assembled, and arrays of heat lamps were mounted above the meter run. The heat lamp arrays were energized in banks in order to simulate different modes of shading of the meter run assembly to determine the influence of solar radiation on flow measurement at low flow rates. Observed flow measurement errors were evaluated by analysis of ultrasonic meter diagnostic log data and through various temperature measurements on the pipe surface and measurements taken at the pipe centerline. The effectiveness of using a stepped thermowell compared to a bare RTD was also evaluated in these tests.
4

Jessop, Colin P. lambda anti-lambda Production in Two-Photon Interactions at CLEO. Office of Scientific and Technical Information (OSTI), May 2003. http://dx.doi.org/10.2172/813097.

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Bodmer, A. R., Q. N. Usmani, and M. Sami. {Lambda} single-particle energies. Office of Scientific and Technical Information (OSTI), August 1995. http://dx.doi.org/10.2172/166465.

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Gourlay, Stephen Alan. Inclusive Production and Polarization of Lambda / anti-Lambda in Hadron Proton Interactions. Office of Scientific and Technical Information (OSTI), January 1985. http://dx.doi.org/10.2172/1433244.

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Gattoni and Olsen. PR-179-10211-R01 Advanced Control Techniques and Sensors for Gas Engines with NSCR. Chantilly, Virginia: Pipeline Research Council International, Inc. (PRCI), July 2012. http://dx.doi.org/10.55274/r0010987.

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High exhaust emissions reduction efficiencies from a spark ignited (SI) internal combustion engine utilizing an NSCR catalyst system requires complex fuel control strategies. The allowable equivalence ratio operating range is very narrow where NSCR systems achieve high exhaust emissions reduction efficiencies of multiple species. Current fuel control technologies utilizing lambda sensor feedback are reported to be unable to sustain these demands for extended operation periods and when transients are introduced. Lambda sensor accuracy is the critical issue with current fuel controllers. The goal of this project was to develop a minimization control algorithm utilizing a Nitrogen Oxide (NOx) sensor installed downstream of the NSCR catalyst system for feedback air/fuel ratio control. When the engine is operated lean, NOx is produced and the NOx sensor responds accordingly. When the engine is operated rich, the NSCR catalyst system produces Ammonia. The NOx sensor has a cross sensitivity to Ammonia and responds as though it has been exposed to NOx. This behavior provides an opportunity for a unique control strategy that allows lambda sensor calibration to be ignored. Testing was performed on a 100kW rated Cummins-Onan generator set that was reconfigured to operate utilizing an electronic gas carburetor (EGC2) with lambda sensor feedback and high reduction efficiency NSCR catalyst system. The control algorithm was programmed utilizing a Labview interface that communicated with the electronic gas carburetor where the fuel trim adjustment was physically made. Improvement under steady state operation was observed. The system was also evaluated during load and fuel composition transients.
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Heredia-De La Cruz, Ivan. Measurement of the $\Lambda^0_b$ lifetime in the exclusive decay $\Lambda^0_b \rightarrow J/\psi \Lambda^0$ with the \D0~detector. Office of Scientific and Technical Information (OSTI), June 2012. http://dx.doi.org/10.2172/1209535.

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9

Staniszewski, Jeffrey M., and Travis A. Bogetti. LAMPAT and LAMPATNL User's Manual. Fort Belvoir, VA: Defense Technical Information Center, September 2012. http://dx.doi.org/10.21236/ada570411.

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Artemov, S. N. Proof Polynomials vs. lambda-terms. Fort Belvoir, VA: Defense Technical Information Center, March 1998. http://dx.doi.org/10.21236/ada344395.

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To the bibliography