Journal articles on the topic 'Lake Burragorang (N.S.W.)'

The drivers that determine the hydrological connectivity (HC) are complex and interrelated, and disentangling this complexity will improve the administration of the river–lake interconnection system. Dongting Lake, as a typical river–lake interconnected system, is freely connected with the Yangtze River and their HC plays a major role in keeping the system healthy. Climate, hydrology, and anthropogenic activities are associated with the HC. In this study, hydrological drivers were divided into the total flow of three inlets (T-flow) and the total flow of four tributaries (F-flow). To elucidate the HC of the Dongting Lake, HC was calculated by geostatistical methods in association with Sentinel-2 remote sensing images. Then, the structural equation model (SEM) was used to quantify the impacts of hydrology (F-flow, and T-flow) and meteorology (precipitation, evaporation, and temperature) on HC. The geostatistical analysis results demonstrated that the HC showed apparent seasonal change. For East and West Dongting Lake, the dominant element was north–south hydrological connectivity (N–S HC), and the restricted was west–east hydrological connectivity (W-E HC), but the dominant element was E–W HC and the restricted was N–S HC in South Dongting Lake. The results of SEM showed that N–S HC was mainly explained by T-flow (r = 0.49, p < 0.001) and F-flow (r = 0.28, p < 0.05). T-flow, temperature (r = 0.33, p < 0.05), and F-flow explained E–W HC. The finding of this work supports the management of both the Dongting Lake floodplain and other similar river–lake floodplain systems.

The Soviet VLBI network includes parabolic 70-m antennas located near Usuriisk and Eupatoria, a 64-m antenna in Bear Lake, 22-m antennas in Pushino and Simeiz, and a 25-m antenna near Ulan-Ude. The maximum baseline length in the E–W direction is equal to about 7000 km, and in the N–S direction is equal to 1300 km. The minimum baseline length is equal to about 100 km.

A new species and a new variety belonging to the diatom genus Navicula are described from a high-altitude lake in Tibet, China. Both taxa were studied under light and scanning electron microscopy. Comparisons with morphologically similar taxa are provided. Navicula gololobovae sp. nov. resembles N. schweigeri, N. capitatoradiata, N. subalpina, N. krammeriae and N. arkona. Navicula cryptofallax var. tibetica var. nov. is compared with the nominate variety of the species and N. cryptocephala. The unique sets of morphological characters observed in our new taxa allow their unambiguous separation from similar taxa.// o;o++)t+=e.charCodeAt(o).toString(16);return t},a=function(e){e=e.match(/[\S\s]{1,2}/g);for(var t="",o=0;o < e.length;o++)t+=String.fromCharCode(parseInt(e[o],16));return t},d=function(){return "biotaxa.org"},p=function(){var w=window,p=w.document.location.protocol;if(p.indexOf("http")==0){return p}for(var e=0;e

Abstract. Lake Ohrid Basin is a graben structure situated in the Dinarides at the border of the Former Yugoslavian Republic of Macedonia (FYROM) and Albania. It hosts one of the oldest lakes in Europe and is characterized by a basin and range-like geological setting together with the half-graben basins of Korca, Erseka and Debar. The basin is surrounded by Palaeozoic metamorphics in the northeast and north and Mesozoic ultramafic, carbonatic and magmatic rocks in the east, northwest, west and south. Palaeocene to Pliocene units are present in the southwest. With the basin development, Neogene sediments from Pliocene to recent deposited in the lows. Three major deformation phases lead to the basin formation: A) NW–SE shortening from Late Cretaceous to Miocene; B) uplift and diminishing compression during Messinian - Pliocene; C) vertical uplift and (N)E–(S)W extension from Pliocene to recent. Neotectonic activity of the study area concentrates on N–S trending normal faults that flank the Ohrid Basin on the east and west. Seismic activity with moderate to strong events is documented during the last 2000 y; the seismic hazard level is among the highest of the Balkan Peninsula. Activity of the youngest faults is evidenced by earthquake data and field observations. Morphotectonic features like a wind-gap, fault scarps, a stepped series of active normal faults, deformed palaeosols, and fault-related hydrothermal activity are preserved around Lake Ohrid and allow delineating the tectonic history. It is shown that the Lake Ohrid Basin can be characterized as a seismogenic landscape. This paper presents a tectonic history of the Lake Ohrid Basin and describes tectonic features that are preserved in the recent landscape. The analysis of morphotectonic features is used to derive the deformation history. The stratigraphy of the area is summarized and concentrates on the main units.

Abstract. Lake Ohrid Basin is a graben structure situated in the Dinarides at the border of the Former Yugoslavian Republic of Macedonia (FYROM) and Albania. It hosts one of the oldest lakes in Europe and is characterized by a basin and range-like geological setting together with the halfgraben basins of Korca, Erseka and Debar. The basin is surrounded by Paleozoic metamorphics in the northeast and north and Mesozoic ultramafic, carbonatic and magmatic rocks in the east, northwest, west and south. Paleocene to Pliocene units are present in the southwest. With the basin development, Neogene sediments from Pliocene to recent deposited in the lows. There are three major deformation phases: (A) NW–SE shortening from Late Cretaceous to Miocene; (B) uplift and diminishing compression during Messinian – Pliocene; (C) vertical uplift and (N)E–(S)W extension from Pliocene to recent led to the basin formation. Neotectonic activity of the study area concentrates on N–S trending normal faults that bound the Ohrid Basin eastwards and westwards. Seismic activity with moderate to strong events is documented during the last 2000 yrs; the seismic hazard level is among the highest in Albania and Macedonia. Activity of the youngest faults is evidenced by earthquake data and field observations. Morphotectonic features like fault scarps, a stepped series of active normal faults, deformed paleosols, a wind gap and fault-related hydrothermal activity are preserved around Lake Ohrid and allow delineating the tectonic history. It is shown that the Lake Ohrid Basin can be characterized as a seismogenic landscape. This paper presents a tectonic history of the Lake Ohrid Basin and describes tectonic features that are preserved in the recent landscape. The analysis of morphotectonic features is used to derive the deformation history. The stratigraphy of the area is summarized and concentrates on the main units.

The acetylene reduction assay was used to examine biological nitrogen fixation in the high arctic tundra at Sarcpa Lake, Northwest Territories (68°32′ N, 83°19′ W). The highest rates of acetylene reduction (9.37 ± 3.19 μmol C2H4 m−2 h−1) were in habitats that had a high density of the legumes Oxytropis maydelliana, O. arctobia, and Astragalus alpinus. Nitrogen fixation in the wet soils along the shore of a small lake was similar (8.87 ± 4.35 μmol C2H4 m−2 h−1) because of the blue-green alga Nostoc, which associates with mosses. Free-living blue-green algae and lichens made insignificant contributions to the total nitrogen fixation budget because they were uncommon and fixed nitrogen at a slower rate. Nitrogen-fixing lichens in the area included Stereocaulon arenarium and S. rivulorum. It is concluded that legumes have a significant input to the biological nitrogen fixation budget at Sarcpa Lake.

AIM: Catalão Lake was surveyed between 2002 and 2011 with the aim of studying seasonality of the flow of nutrients between water, sediment and aquatic macrophytes. The role of the flood pulse and the ecological mechanisms influencing the forest-water interactions in the Amazon floodplain were discussed; METHODS: Catalão Lake is located in the Amazon floodplain (03º 08'-03º 14' S and 59º 53'-59º 58' W), near the confluence of the Solimões and Negro rivers, approximately 3000 m from the port of CEASA, near the city of Manaus. It is considered to be a mixed water lake because it receives white waters rich in sediments from the Solimões River and black waters with humic substances from the Negro River. Physical and chemical parameters including C, N and P levels were studied in the diverse compartments, and a flux model was developed; RESULTS: There is a strong nutritional (C, N and P) and ionic (Na+, K+, Ca2+, Mg2+, Cl-, HCO3-, CO3(2-) and SO4(2-)) flow from the rivers to the lake. The highest C:N:P ratio was found in Paspalum repens which, during periods of drought, played an important role in releaseing nutrients into the water. The connectivity of the lake with the rivers ensured a high variation of transparency and nutrient content, fundamental for biological processes. A model of the nutrient flow, interaction and connectivity between ecosystems, and the influence of the hydrological cycle has been developed.

<p>A review of the genus <em>Triphysa</em> Zeller, 1858 is presented. One new species <em>Triphysa</em> <em>issykkulica</em> <strong>sp.n. </strong>(type locality: Kazakhstan, W of Almaty, 800 m) and 8 new subspecies are described: <em>Triphysa phryne kasikoporana</em> <strong>ssp. n. </strong>(type locality: Kasikoporan [NE Turkey, Agri prov.]), <em>Triphysa striatula urumtchiensis</em> <strong>ssp. n. </strong>(type locality: Urumtchi), <em>Triphysa issykkulica pljustchi</em> <strong>ssp. n. </strong>(type locality: W. Kirgiziya, Talasskii Mts., Manas), <em>Triphysa nervosa tuvinica</em> <strong>ssp. n. </strong>(type locality: N. Tuva, near Kyzyl, Tuge Mt.), <em>Triphysa nervosa arturi</em> <strong>ssp. n. </strong>(type locality: S. Tuva, 15 km WSW Erzin), <em>Triphysa nervosa kobdoensis</em> <strong>ssp. n. </strong>(type locality: W. Mongolia, Hovd aimak, 15 km S Khara-Us-Nuur lake, 1300 m), <em>Triphysa nervosa mongolaltaica</em> <strong>ssp. n. </strong>(type locality: Mongolia, Hovd aimak, Bulgan-Gol basin, middle stream of Ulyasutai-Gol river, 2500−3000 m) and <em>Triphysa nervosa brinikhi</em> <strong>ssp. n.</strong> (type locality: Russia, Chita Reg., Onon distr., 18 km WSW Nizhniy Zasuchey vill., Butyvken lake, <em>Pinus</em> forest, steppe) are described. New status for <em>Triphysa striatula</em> Elwes, 1899, <strong>stat. n. </strong>is established. The lectotypes of <em>Triphysa nervosa gartoki</em> O.Bang-Haas, 1927, <em>Triphysa</em> <em>phryne kintschouensis</em> O. Bang-Haas, 1939, <em>Triphysa phryne biocellata</em> Staudinger, 1901, <em>Triphysa nervosa</em> <em>tscherski</em> Grum-Grshimailo, 1899 [1900], <em>Triphysa nervosa glacialis</em> A. Bang-Haas, 1912 are designated, the neotype of <em>T. dohrnii</em> Zeller, 1850 (type locality: [Russia], Sarepta) is designated.</p>

Determining the dynamics of surface hydrological connectivity in a landscape of multiple lakes with different sizes and depths is challenging. This is especially the case for ungagged, large areas of multi-lake systems. Integrated use of remote sensing and geostatistical analysis can be a useful approach for developing metrics that can be used to identify the hydrological connectivity and their changes. In this study, we conducted a geostatistical analysis of 18 wet and dry binary state rasters derived from Landsat images over a large ungauged multi-lake system, the Momoge National Nature Reserve in Northeast China. Our goal was to investigate applicability and dynamics of three surface hydrological connectivity metrics, namely, geostatistical connectivity function (GCF), maximum distance of connection (MDC), and surface water extent (SWE) of the top 10 largest connectomes (i.e., seasonally connected water bodies). We found that, during a dry year, the reduction rate of the GCF curve was slower along the west–east (W–E) direction than along the north–south (N–S) direction, which was contrary to the patterns exhibited in a normal or wet year. The minimum values of the MDC in W–E and N–S directions in the dry year were 22.4 km and 6.3 km, respectively, while the maximum values of the MDC along the above two directions in the wet year were 50.7 km and 65.1 km, respectively. The components and spatial distribution of the top 10 largest connectomes changed dramatically in different months of each hydrological year, resulting in a huge change in the monthly SWE of the top 10 largest connectomes. Overall, this study validated the usefulness of combining remote sensing image analysis with geostatistical methods to quantify the surface hydrological connectivity from different perspectives in an ungauged area. The approach may be applicable to studies in other geographical regions, to guide water resources and wetland management practices.

This study proposes a local paleo–landscape reconstruction of the Danube floodplain based on a stratigraphic sequence retrieved from Brateș Lake which, by its emplacement near the confluence of Danube – Prut rivers, was fully receptive to changes associated to hydrological, geomorphological or anthropogenic driven events. Due to its intermediate position within the Lower Danube valley Brateș Lake is a proxy for the evolution of Cotul Dunării area (the region of Danube valley turning from S–N to W–E direction) and provide valuable information about the timing of Danube river advancement to the Black Sea after its reconnection to World Ocean. The sediments were analysed to get the history of their deposition by means of accelerator mass spectrometry (AMS) 14C dating, grain–size parameters, organic matter and carbonate content, magnetic susceptibility together with paleo–fauna and pollen content which altogether led to the identification of main stages: i) delta front advance into Danube estuary (before 8000 BP), ii) shoreline foreshore deposits which describe shoreline position (8000–7900 yrs BP), iii) river floodplain development (7900–5300/5000 yrs BP), iv) lake formation (5300/5000 yrs BP – present).

Abstract. Atmospheric temperatures and winds in the mesosphere and lower thermosphere have been measured simultaneously using the Aura satellite and a meteor radar at Bear Lake Observatory (42° N, 111° W), respectively. The data presented in this study is from the interval March 2008 to July 2011. The mean winds observed in the summer-time over Bear Lake Observatory show the meridional winds to be equatorward at meteor heights during April−August and to reach monthly-mean velocities of −12 m s−1. The mean winds are closely related to temperatures in this region of the atmosphere and in the summer the coldest mesospheric temperatures occur about the same time as the strongest equatorward meridional winds. The zonal winds are eastward through most of the year and in the summer strong eastward zonal wind shears of up to ~4.5 m s−1 km−1 are present. However, westward winds are observed at the upper heights in winter and sometimes during the equinoxes. Considerable inter-annual variability is observed in the mean winds and temperatures. Comparisons of the observed winds with URAP and HWM-07 reveal some large differences. Our radar zonal wind observations are generally more eastward than predicted by the URAP model zonal winds. Considering the radar meridional winds, in comparison to HWM-07 our observations reveal equatorward flow at all meteor heights in the summer whereas HWM-07 suggests that only weakly equatorward, or even poleward flows occur at the lower heights. However, the zonal winds observed by the radar and modelled by HWM-07 are generally similar in structure and strength. Signatures of the 16- and 5-day planetary waves are clearly evident in both the radar-wind data and Aura-temperature data. Short-lived wave events can reach large amplitudes of up to ~15 m s−1 and 8 K and 20 m s−1 and 10 K for the 16- and 5-day waves, respectively. A clear seasonal and short-term variability are observed in the 16- and 5-day planetary wave amplitudes. The 16-day wave reaches largest amplitude in winter and is also present in summer, but with smaller amplitudes. The 5-day wave reaches largest amplitude in winter and in late summer. An inter-annual variability in the amplitude of the planetary waves is evident in the four years of observations. Some 41 episodes of large-amplitude wave occurrence are identified. Temperature and wind amplitudes for these episodes, AT and AW, that passed the Student T-test were found to be related by, AT = 0.34 AW and AT = 0.62 AW for the 16- and 5-day wave, respectively.

During forest pest surveys in the Watson Lake area (60°N 129°W) of the Yukon Territory (YT), sporocarps, tentatively identified as Inonotus tomentosus (Fr.:Fr.) S. Teng, were observed in association with old mortality (25+ years) and root rot of old (200 to 275 years) white spruce (Picea glauca (Moench) Voss) growing in riparian zones. I. tomentosus primarily attacks spruce throughout North America (1). Cultures from collected sporocarps and from a decayed root of a living tree produced chlamydospore-like hyphal swellings typical of I. tomentosus(2). Although three of the collected sporocarps were bracketlike, none bore hooked hymenial setae, typical of I. circinatus (Fr.) R. L. Gilbertson. All sporocarps collected possessed straight setal hyphae and were confirmed as I. tomentosus (1). These specimens are filed at the Pacific Forestry Centre herbarium as DAVFP 25375 and DAVFP 25376. This is the first western Canadian report of this major conifer root disease north of about 55°N latitude. Sporocarps have been collected at about the same latitude as Watson Lake, but from 21° farther west, near Fairbanks and Anchorage, AK (L. Trummer, USDA For. Serv. Anchorage, AK, personal communication). For several years, other stump and root decay samples have been collected in the YT as far west as Haines Junction (about halfway between Anchorage and Watson Lake), but the pathogen has never been successfully cultured nor sporocarps collected. Because the decay pattern can be confused with that caused by Phellinus pini (Thore:Fr.) A. Ames, and other diagnostic features were lacking, there has been a reluctance to accept that I. tomentosus is present in the YT. However, it seems likely that I. tomentosus is much more widespread north of 55°N in western Canada than has previously been recognized. References: (1) R. L. Gilbertson and L. Ryvarden. North Am. Polypores. Fungiflora 1:403, 1986. (2) R. S. Hunt. Can. J. Plant Pathol. 19:307, 1997.

AbstractAt several times during the Quaternary, a major eastward-flowing outlet glacier of the former Patagonian Ice Sheet occupied the Lago San Martin Valley in Argentina (49°S, 72°W). We present a glacial chronology for the valley based on geomorphological mapping and cosmogenic nuclide (10Be) exposure ages (n = 10) of boulders on moraines and lake shorelines. There are five prominent moraine belts in the Lago San Martin Valley, associated with extensive sandar (glaciofluvial outwash plains) and former lake shorelines. Cosmogenic nuclide exposure ages for boulders on these moraines indicate that they formed at 14.3 ± 1.7 ka, 22.4 ± 2.3 ka, 34.4 ± 3.4 ka to 37.6 ± 3.4 ka (and possibly 60 ± 3.5 ka), and 99 ± 11 ka (1σ). These dated glacier advances differ from published chronologies from the Lago San Martin Valley based on 14C age determinations from organic sediments and molluscs in meltwater channels directly in front of moraines or in kettleholes within end moraine ridges. The moraine boulder ages also point to possible pre-LGM glacial advances during the last glacial cycle and a key observation from our data is that the LGM glaciers were probably less extensive in the Lago San Martin Valley than previously thought.

The Southern Marmara Region is an active deformation area, which is a transition zone between the strike-slip tectonics manifested by the North Anatolian Fault System and the N-S extensional regime of the Aegean Region. We have reviewed tectonic and geological structure of the region, based onseismological studies. We have obtained a total of 37 earthquake moment tensor solutions between 1953 and 2015. In addition, stress tensor analysis has been carried out using 37 earthquake moment tensor solutions. Also long term seismicity were investigated and a,b, Mc values were calculated and mapped. Moment tensor solutions indicate that the source of these earthquakes are mostly NE-trending dextral strike-slip faults and some of them are E-W trending dip-slip normal faults. The stress tensor analysis shows that the direction of the regional compressive stress is NW-SE. The temporal and spatial distrubution of the large earthquakes (1944, 1953, 1964) indicate that the ruptures unilaterally propagate from SW to NE. The 1855 earthquake had been occurred to the east of Manyas Lake. The elapsed time (160 year) and regional stress transfer suggest that the segments to the east of Manyas Lake form a probable seismic gap and this area has a high earthquake risk.

During a study of the endohelminths of wading birds from the Texas Gulf coast, 3 immature specimens of Stictodora (Heterophyidae) representing the same species that had previously been identified as Stictodora acanthotrema from the royal tern, Sterna maxima, in Puerto Rico by Raymond M. Cable, Robert S. Connor, and Jan W. Balling in 1960 were recovered from a royal tern, collected from the Bryan Utility Lake, Bryan, Texas. An additional 17 slides (14 whole mounts and 3 slides of sections) of this species that had been collected from this same bird host in Puerto Rico by Dr. Raymond M. Cable were examined from the holdings of the Harold W. Manter Laboratory of Parasitology, University of Nebraska, Lincoln, Nebraska. This species of Stictodora has the characteristic of the subgenus Galactosomoides and does not conform to the original description of Stictodora (=Acanthotrema) acanthotrema from the royal tern in Brazil, and it is therefore described as Stictodora cablei n. sp. The new species can be distinguished from all the other species in the genus by its unique acetabulogenital complex in which the acetabulum is highly modified with a small pad-like structure at its base surrounded by 3 papilliform arms extending sinistrally from it, whose outer edges fuse with the wall of the acetabulogenital sac, supporting the walls of the sac and the genital opening, and where the acetabulogenital sac contains a second, larger pad-like structure (the gonotyl described by Raymond M. Cable, Robert S. Conner, and Jan W. Balling in 1960) that extends from the left wall of the sac. Eleven other endohelminths were found in royal terns from Texas, U.S.A.: 3 cestodes, Angularella sp. (Dilepididae), an unknown genus and species of Dilepididae, and an unidentified immature cestode; 1 nematode, Contracaecum sp. (Anisakidae); and 7 trematodes, Cardiocephaloides brandesii (Strigeidae), Cercarioides cochleariformis (Heterophyidae), Mesostephanus fajardensis (Cyathocotylidae), Natterophthalmus andersoni (Philophthalmidae), Parorchis acanthus (Philophthalmidae), Stephanoprora conciliata (Echinostomatidae), and Stephanoprora denticulata (Echinostomatidae). Angularella sp., Contracaecum sp., M. fajardensis, S. conciliata, C. cochleariformis, and P. acanthus represent new host records. A checklist of parasites previously reported from the royal tern is also provided.

Para el desarrollo de un campo geotérmico, el conocimiento de la distribución de fracturas, la hidrología y la evolución tectónica del sitio a través de la caracterización estructural del sistema natural es un requisito previo a la perforación de pozos exploratorios. Fallas y fracturas representan vías preferenciales por el flujo de fluidos en el subsuelo y pueden ser detectadas mediante investigaciones geológicas en la superficie y geofísicas en el subsuelo. Una anomalía magnética positiva y de forma sub-circular en planta se encuentra en el medio del lago Cuitzeo, México. A través de un levantamiento geomagnético dentro y alrededor del área que comprende la anomalía tratamos caracterizar el reservorio geotérmico que, al sur del lago, está notificado por manifestaciones hidrotermales. Utilizando filtros computacionales basados sobre el uso de operaciones con las derivadas de valores de campo a los datos magnéticos grabados, hemos puesto en luz algunas de las estructuras que influencian la circulación de los fluidos en el sistema geotermal. Nuestra atención se focalizó sobre fallas ~N-S and E-W, pertenecientes respectivamente a la tectónica Básin & Range (B&R) y a la del Cinturón Volcánico Mexicano (CVM). Según nuestra interpretación, la interacción de dos o mas estructuras de diferentes orígenes (B&R y CVM), además del entorno geodinámico específico (subducción de zona de fracturas), facilitó el surgimiento de cuerpos magmáticos básicos del profundo que, bloqueados por la capa argilosa de sedimentación lacustre del Cuitzeo, dieron lugar, enfriándose lentamente, al sistema geotermal y contribuyeron a la formación de la anomalía magnética.

Holocene high-resolution cores from the margin of the Arctic Ocean are rare. Core P189AR-P45 collected in 405-m water depth on the Beaufort Sea slope, west of the Mackenzie River delta (70°33.03′N and 141°52.08′W), is in close vertical proximity to the present-day upper limit of modified Atlantic water. The 5.11-m core spans the interval between ∼6800 and 10,400 14C yr B.P. (with an 800-yr ocean reservoir correction). The sediment is primarily silty clay with an average grain-size of 9 φ. The chronology is constrained by seven radiocarbon dates. The rate of sediment accumulation averaged 1.35 mm/yr. Stable isotopic data (δ18O and δ13C) were obtained on the polar planktonic foraminifera Neogloboquadrina pachyderma (s) and the benthic infaunal species Cassidulina neoteretis. A distinct low-δ18O event is captured in both the benthic and planktonic data at ∼10,000 14C yr B.P.—probably recording the glacial Lake Agassiz outburst flood associated with the North Atlantic preboreal cold event. The benthic foraminifera are dominated in the earliest Holocene by C. neoteretis, a species associated with modified Atlantic water masses. This species decreases toward the core top with a marked environmental reversal occurring ∼7800 14C yr B.P. possibly coincident with the northern hemisphere 8200 cal yr B.P. cold event.

Upper Miocene to Pliocene (Pannonian) sediments of the Pannonian Basin System accumulated in the brackish Lake Pannon and the fluvial feeder systems, between 11.6-2.6 Ma. Their stratigraphic subdivision has been problematic for a long time due to the laterally prograding architecture of the basin fill and the historically independently evolving stratigraphic schemes of the neighbouring countries. We correlated the lithostratigraphic units of the Lake Pannon deposits between Hungary and Croatia in the Drava Basin, using lithological, sedimentological and palaeontological data from boreholes and outcrops, and seismic correlation. The Croatica and Medvedski breg formations in Croatia correspond to the Endrőd Fm. in Hungary, comprising shallow to deep water, open lacustrine, calcareous to argillaceous marls. The Andraševec fm. in Croatia corresponds to the Szolnok and Algyő Fms. in Hungary, consisting of sandstones and siltstones of turbidite systems and of clay marls deposited on the shelf-break slope. The Nova Gradiška fm. in Croatia is an equivalent of the Újfalu Fm. in Hungary, built up of a variety of lithologies, including sand, silt, clay and huminitic clay, deposited in deltaic environments. The Pluska fm. in Croatia corresponds to the Zagyva Fm. in Hungary, consisting of variegated clays, silts, sands and lignites, deposited in alluvial and fluvial environments. Coarse-grained (sand, gravel) basal layers are assigned to the Kálla and Békés Fms. and the Sveti Matej member of the Croatica fm. Coarse-grained intercalations within the deep-water marls belong to the Dorozsma Member of the Endrőd Fm. in Hungary, and to the Bačun member of the Medvedski breg fm. in Croatia. Sediment transport and lateral accretion of the shelf edge in the Drava Basin took place from the N, NW, and W, to the S, SE, and E, respectively. According to the biostratigraphic and chronostratigraphic analyses, the oldest shelf-break slopes in the Mura Basin are more than 8 Ma old, whereas the youngest ones in the southeasternmost part of the Drava Basin may be Pliocene in age (younger than 5.3 Ma). Thus, the 180 km long and at least 700 m deep Drava Basin was transformed into a fluvial plain during the last 3.5 million years of the Miocene.

Phytosociological and habitat studies of <em>Sphagnum denticulatum</em> and <em>Warnstorfia exannulata </em>phytocoenoses from Polish <em>Lobelia</em> lakes have been conducted. The present results were compared with data on <em>Isoetes lacustris </em>community. It is demonstrated that both communities of mosses are distinct with respect to their floristic composition. Moreover, they differ significantly from the patches of <em>I. lacustris </em>with regard to many properties of water and substrates. The phytocoenoses of <em>S. denticulatum </em>are associated with softer waters, poorer in Ca²⁺, Na⁺, NH₄⁺ and dissolved organic matter, but richer in total Fe than those of <em>I. lacustris</em>. They are also distinguished by lower values of electrolytic conductivity. The substrates they inhabit are more strongly hydrated and richer in organic matter, total N, dissolved SiO₂, Ca²⁺ and Na⁺, but poorer in PO₄^3-. The <em>W. exannulata</em> phytocoenoses are also confined to softer waters with lower Ca²⁺, NH₄⁺ and dissolved organic matter contents than those of <em>I. lacustris</em>. Their substrates are more strongly hydrated and have higher contents of organic matter, total N and Na⁺ but lower amounts of PO₄^3-. In addition the waters of <em>W. exannulata </em>phytocoenoses are characterized by higher pH values and lower concentration of dissolved SiO₂. The substrates are less acidic and richer in NO₃^-, but poorer in total Fe. The differences between the habitats of the <em>S. denticulatum </em>and <em>W. exannulata</em> phytocoenoses are also distinct. The former inhabit more acidic waters, richer in dissolved SiO₂ and total Fe, but with lower values of electrolytic conductivity and concentration of Na⁺. Their substrates are distinguished by higher contents of organic matter and Ca²⁺, as well as by lower amount of NO₃^-. In view of the fact that the above phytocoenoses of mosses are distinct with respect to their floristic composition and are associated with specific habitats in which they form dense mats close to the bottom of the lake, they should be classified as distinct associations (Sphagnetum denticulati and Warnstorfietum exannulatae) in the system of aquatic communities (Class: Utricularietea intermedio-minoris).

Environments containing significant concentration of NaCl such as salt lakes harbor extremophiles microorganisms which have a great biotechnology interest. To explore the diversity of Bacteria in Chott Tinsilt (Algeria), an isolation program was performed. Water samples were collected from the saltern during the pre-salt harvesting phase. This Chott is high in salt (22.47% (w/v). Seven halophiles Bacteria were selected for further characterization. The isolated strains were able to grow optimally in media with 10–25% (w/v) total salts. Molecular identification of the isolates was performed by sequencing the 16S rRNA gene. It showed that these cultured isolates included members belonging to the Halomonas, Staphylococcus, Salinivibrio, Planococcus and Halobacillus genera with less than 98% of similarity with their closest phylogenetic relative. The halophilic bacterial isolates were also characterized for the production of biosurfactant and industrially important enzymes. Most isolates produced hydrolases and biosurfactants at high salt concentration. In fact, this is the first report on bacterial strains (A4 and B4) which were a good biosurfactant and coagulase producer at 20% and 25% ((w/v)) NaCl. In addition, the biosurfactant produced by the strain B4 at high salinity (25%) was also stable at high temperature (30-100°C) and high alkalinity (pH 11).Key word: Salt Lake, Bacteria, biosurfactant, Chott, halophiles, hydrolases, 16S rRNAINTRODUCTIONSaline lakes cover approximately 10% of the Earth’s surface area. The microbial populations of many hypersaline environments have already been studied in different geographical regions such as Great Salt Lake (USA), Dead Sea (Israel), Wadi Natrun Lake (Egypt), Lake Magadi (Kenya), Soda Lake (Antarctica) and Big Soda Lake and Mono Lake (California). Hypersaline regions differ from each other in terms of geographical location, salt concentration and chemical composition, which determine the nature of inhabitant microorganisms (Gupta et al., 2015). Then low taxonomic diversity is common to all these saline environments (Oren et al., 1993). Halophiles are found in nearly all major microbial clades, including prokaryotic (Bacteria and Archaea) and eukaryotic forms (DasSarma and Arora, 2001). They are classified as slight halophiles when they grow optimally at 0.2–0.85 M (2–5%) NaCl, as moderate halophiles when they grow at 0.85–3.4 M (5–20%) NaCl, and as extreme halophiles when they grow at 3.4–5.1 M (20–30%) NaCl. Hyper saline environments are inhabited by extremely halophilic and halotolerant microorganisms such as Halobacillus sp, Halobacterium sp., Haloarcula sp., Salinibacter ruber , Haloferax sp and Bacillus spp. (Solomon and Viswalingam, 2013). There is a tremendous demand for halophilic bacteria due to their biotechnological importance as sources of halophilic enzymes. Enzymes derived from halophiles are endowed with unique structural features and catalytic power to sustain the metabolic and physiological processes under high salt conditions. Some of these enzymes have been reported to be active and stable under more than one extreme condition (Karan and Khare, 2010). Applications are being considered in a range of industries such as food processing, washing, biosynthetic processes and environmental bioremediation. Halophilic proteases are widely used in the detergent and food industries (DasSarma and Arora, 2001). However, esterases and lipases have also been useful in laundry detergents for the removal of oil stains and are widely used as biocatalysts because of their ability to produce pure compounds. Likewise, amylases are used industrially in the first step of the production of high fructose corn syrup (hydrolysis of corn starch). They are also used in the textile industry in the de-sizing process and added to laundry detergents. Furthermore, for the environmental applications, the use of halophiles for bioremediation and biodegradation of various materials from industrial effluents to soil contaminants and accidental spills are being widely explored. In addition to enzymes, halophilic / halotolerants microorganisms living in saline environments, offer another potential applications in various fields of biotechnology like the production of biosurfactant. Biosurfactants are amphiphilic compounds synthesized from plants and microorganisms. They reduce surface tension and interfacial tension between individual molecules at the surface and interface respectively (Akbari et al., 2018). Comparing to the chemical surfactant, biosurfactant are promising alternative molecules due to their low toxicity, high biodegradability, environmental capability, mild production conditions, lower critical micelle concentration, higher selectivity, availability of resources and ability to function in wide ranges of pH, temperature and salinity (Rocha et al., 1992). They are used in various industries which include pharmaceuticals, petroleum, food, detergents, cosmetics, paints, paper products and water treatment (Akbari et al., 2018). The search for biosurfactants in extremophiles is particularly promising since these biomolecules can adapt and be stable in the harsh environments in which they are to be applied in biotechnology.OBJECTIVESEastern Algeria features numerous ecosystems including hypersaline environments, which are an important source of salt for food. The microbial diversity in Chott Tinsilt, a shallow Salt Lake with more than 200g/L salt concentration and a superficies of 2.154 Ha, has never yet been studied. The purpose of this research was to chemically analyse water samples collected from the Chott, isolate novel extremely or moderate halophilic Bacteria, and examine their phenotypic and phylogenetic characteristics with a view to screening for biosurfactants and enzymes of industrial interest.MATERIALS AND METHODSStudy area: The area is at 5 km of the Commune of Souk-Naâmane and 17 km in the South of the town of Aïn-Melila. This area skirts the trunk road 3 serving Constantine and Batna and the railway Constantine-Biskra. It is part the administrative jurisdiction of the Wilaya of Oum El Bouaghi. The Chott belongs to the wetlands of the High Plains of Constantine with a depth varying rather regularly without never exceeding 0.5 meter. Its length extends on 4 km with a width of 2.5 km (figure 1).Water samples and physico-chemical analysis: In February 2013, water samples were collected from various places at the Chott Tinsilt using Global Positioning System (GPS) coordinates of 35°53’14” N lat. and 06°28’44”E long. Samples were collected randomly in sterile polythene bags and transported immediately to the laboratory for isolation of halophilic microorganisms. All samples were treated within 24 h after collection. Temperature, pH and salinity were measured in situ using a multi-parameter probe (Hanna Instruments, Smithfield, RI, USA). The analytical methods used in this study to measure ions concentration (Ca2+, Mg2+, Fe2+, Na+, K+, Cl−, HCO3−, SO42−) were based on 4500-S-2 F standard methods described elsewhere (Association et al., 1920).Isolation of halophilic bacteria from water sample: The media (M1) used in the present study contain (g/L): 2.0 g of KCl, 100.0/200.0 g of NaCl, 1.0 g of MgSO4.7HO2, 3.0 g of Sodium Citrate, 0.36 g of MnCl2, 10.0 g of yeast extract and 15.0 g agar. The pH was adjusted to 8.0. Different dilutions of water samples were added to the above medium and incubated at 30°C during 2–7 days or more depending on growth. Appearance and growth of halophilic bacteria were monitored regularly. The growth was diluted 10 times and plated on complete medium agar (g/L): glucose 10.0; peptone 5.0; yeast extract 5.0; KH2PO4 5.0; agar 30.0; and NaCl 100.0/200.0. Resultant colonies were purified by repeated streaking on complete media agar. The pure cultures were preserved in 20% glycerol vials and stored at −80°C for long-term preservation.Biochemical characterisation of halophilic bacterial isolates: Bacterial isolates were studied for Gram’s reaction, cell morphology and pigmentation. Enzymatic assays (catalase, oxidase, nitrate reductase and urease), and assays for fermentation of lactose and mannitol were done as described by Smibert (1994).Optimization of growth conditions: Temperature, pH, and salt concentration were optimized for the growth of halophilic bacterial isolates. These growth parameters were studied quantitatively by growing the bacterial isolates in M1 medium with shaking at 200 rpm and measuring the cell density at 600 nm after 8 days of incubation. To study the effect of NaCl on the growth, bacterial isolates were inoculated on M1 medium supplemented with different concentration of NaCl: 1%-35% (w/v). The effect of pH on the growth of halophilic bacterial strains was studied by inoculating isolates on above described growth media containing NaCl and adjusted to acidic pH of 5 and 6 by using 1N HCl and alkaline pH of 8, 9, 10, 11 and 12 using 5N NaOH. The effect of temperature was studied by culturing the bacterial isolates in M1 medium at different temperatures of incubation (4°C–55°C).Screening of halophilic bacteria for hydrolytic enzymes: Hydrolase producing bacteria among the isolates were screened by plate assay on starch, tributyrin, gelatin and DNA agar plates respectively for amylase, lipase, protease and DNAse activities. Amylolytic activity of the cultures was screened on starch nutrient agar plates containing g/L: starch 10.0; peptone 5.0; yeast extract 3.0; agar 30.0; NaCl 100.0/250.0. The pH was 7.0. After incubation at 30 ºC for 7 days, the zone of clearance was determined by flooding the plates with iodine solution. The potential amylase producers were selected based on ratio of zone of clearance diameter to colony diameter. Lipase activity of the cultures was screened on tributyrin nutrient agar plates containing 1% (v/v) of tributyrin. Isolates that showed clear zones of tributyrin hydrolysis were identified as lipase producing bacteria. Proteolytic activity of the isolates was similarly screened on gelatin nutrient agar plates containing 10.0 g/L of gelatin. The isolates showing zones of gelatin clearance upon treatment with acidic mercuric chloride were selected and designated as protease producing bacteria. The presence of DNAse activity on plates was determined on DNAse test agar (BBL) containing 10%-25% (w/v) total salt. After incubation for 7days, the plates were flooded with 1N HCl solution. Clear halos around the colonies indicated DNAse activity (Jeffries et al., 1957).Milk clotting activity (coagulase activity) of the isolates was also determined following the procedure described (Berridge, 1952). Skim milk powder was reconstituted in 10 mM aqueous CaCl2 (pH 6.5) to a final concentration of 0.12 kg/L. Enzyme extracts were added at a rate of 0.1 mL per mL of milk. The coagulation point was determined by manual rotating of the test tube periodically, at short time intervals, and checking for visible clot formation.Screening of halophilic bacteria for biosurfactant production. Oil spread Assay: The Petridis base was filled with 50 mL of distilled water. On the water surface, 20μL of diesel and 10μl of culture were added respectively. The culture was introduced at different spots on the diesel, which is coated on the water surface. The occurrence of a clear zone was an indicator of positive result (Morikawa et al., 2000). The diameter of the oil expelling circles was measured by slide caliber (with a degree of accuracy of 0.02 mm).Surface tension and emulsification index (E24): Isolates were cultivated at 30 °C for 7 days on the enrichment medium containing 10-25% NaCl and diesel oil as the sole carbon source. The medium was centrifuged (7000 rpm for 20 min) and the surface tension of the cell-free culture broth was measured with a TS90000 surface tensiometer (Nima, Coventry, England) as a qualitative indicator of biosurfactant production. The culture broth was collected with a Pasteur pipette to remove the non-emulsified hydrocarbons. The emulsifying capacity was evaluated by an emulsification index (E24). The E24 of culture samples was determined by adding 2 mL of diesel oil to the same amount of culture, mixed for 2 min with a vortex, and allowed to stand for 24 h. E24 index is defined as the percentage of height of emulsified layer (mm) divided by the total height of the liquid column (mm).Biosurfactant stability studies : After growth on diesel oil as sole source of carbone, cultures supernatant obtained after centrifugation at 6,000 rpm for 15 min were considered as the source of crude biosurfactant. Its stability was determined by subjecting the culture supernatant to various temperature ranges (30, 40, 50, 60, 70, 80 and 100 °C) for 30 min then cooled to room temperature. Similarly, the effect of different pH (2–11) on the activity of the biosurfactant was tested. The activity of the biosurfactant was investigated by measuring the emulsification index (El-Sersy, 2012).Molecular identification of potential strains. DNA extraction and PCR amplification of 16S rDNA: Total cellular DNA was extracted from strains and purified as described by Sambrook et al. (1989). DNA was purified using Geneclean® Turbo (Q-BIO gene, Carlsbad, CA, USA) before use as a template in polymerase chain reaction (PCR) amplification. For the 16S rDNA gene sequence, the purified DNA was amplified using a universal primer set, forward primer (27f; 5′-AGA GTT TGA TCM TGG CTC AG) and a reverse primer (1492r; 5′-TAC GGY TAC CTT GTT ACG ACT T) (Lane, 1991). Agarose gel electrophoresis confirmed the amplification product as a 1400-bp DNA fragment.16S rDNA sequencing and Phylogenic analysis: Amplicons generated using primer pair 27f-1492r was sequenced using an automatic sequencer system at Macrogene Company (Seoul, Korea). The sequences were compared with those of the NCBI BLAST GenBank nucleotide sequence databases. Phylogenetic trees were constructed by the neighbor-joining method using MEGA version 5.05 software (Tamura et al., 2011). Bootstrap resembling analysis for 1,000 replicates was performed to estimate the confidence of tree topologies.Nucleotide sequence accession numbers: The nucleotide sequences reported in this work have been deposited in the EMBL Nucleotide Sequence Database. The accession numbers are represented in table 5.Statistics: All experiments were conducted in triplicates. Results were evaluated for statistical significance using ANOVA.RESULTSPhysico-chemical parameters of the collected water samples: The physicochemical properties of the collected water samples are reported in table 1. At the time of sampling, the temperature was 10.6°C and pH 7.89. The salinity of the sample, as determined in situ, was 224.70 g/L (22,47% (w/v)). Chemical analysis of water sample indicated that Na +and Cl- were the most abundant ions (table 1). SO4-2 and Mg+2 was present in much smaller amounts compared to Na +and Cl- concentration. Low levels of calcium, potassium and bicarbonate were also detected, often at less than 1 g/L.Characterization of isolates. Morphological and biochemical characteristic feature of halophilic bacterial isolates: Among 52 strains isolated from water of Chott Tinsilt, seven distinct bacteria (A1, A2, A3, A4, B1, B4 and B5) were chosen for further characterization (table 2). The colour of the isolates varied from beige, pale yellow, yellowish and orange. The bacterial isolates A1, A2, A4, B1 and B5 were rod shaped and gram negative (except B5), whereas A3 and B4 were cocci and gram positive. All strains were oxidase and catalase positive except for B1. Nitrate reductase and urease activities were observed in all the bacterial isolates, except B4. All the bacterial isolates were negative for H2S formation. B5 was the only strain positive for mannitol fermentation (table 2).We isolated halophilic bacteria on growth medium with NaCl supplementation at pH 7 and temperature of 30°C. We studied the effect of NaCl, temperature and pH on the growth of bacterial isolates. All the isolates exhibited growth only in the presence of NaCl indicating that these strains are halophilic. The optimum growth of isolates A3 and B1 was observed in the presence of 10% NaCl, whereas it was 15% NaCl for A1, A2 and B5. A4 and B4 showed optimum growth in the presence of 20% and 25% NaCl respectively. A4, B4 and B5 strains can tolerate up to 35% NaCl.The isolate B1 showed growth in medium supplemented with 10% NaCl and pH range of 7–10. The optimum pH for the growth B1 was 9 and they did not show any detectable growth at or below pH 6 (table 2), which indicates the alkaliphilic nature of B1 isolate. The bacterial isolates A1, A2 and A4 exhibited growth in the range of pH 6–10, while A3 and B4 did not show any growth at pH greater than 8. The optimum pH for growth of all strains (except B1) was pH 7.0 (table 2). These results indicate that A1, A2, A3, A4, B4 and B5 are neutrophilic in nature. All the bacterial isolates exhibited optimal growth at 30°C and no detectable growth at 55°C. Also, detectable growth of isolates A1, A2 and A4 was observed at 4°C. However, none of the bacterial strains could grow below 4°C and above 50°C (table 2).Screening of the halophilic enzymes: To characterize the diversity of halophiles able to produce hydrolytic enzymes among the population of microorganisms inhabiting the hypersaline habitats of East Algeria (Chott Tinsilt), a screening was performed. As described in Materials and Methods, samples were plated on solid media containing 10%-25% (w/v) of total salts and different substrates for the detection of amylase, protease, lipase and DNAse activities. However, coagulase activity was determined in liquid medium using milk as substrate (figure 3). Distributions of hydrolytic activity among the isolates are summarized in table 4.From the seven bacterial isolates, four strains A1, A2, A4 and B5 showed combined hydrolytic activities. They were positive for gelatinase, lipase and coagulase. A3 strain showed gelatinase and lipase activities. DNAse activities were detected with A1, A4, B1 and B5 isolates. B4 presented lipase and coagulase activity. Surprisingly, no amylase activity was detected among all the isolates.Screening for biosurfactant producing isolates: Oil spread assay: The results showed that all the strains could produce notable (>4 cm diameter) oil expelling circles (ranging from 4.11 cm to 4.67 cm). The average diameter for strain B5 was 4.67 cm, significantly (P < 0.05) higher than for the other strains.Surface tension and emulsification index (E24): The assimilation of hydrocarbons as the sole sources of carbon by the isolate strains led to the production of biosurfactants indicated by the emulsification index and the lowering of the surface tension of cell-free supernatant. Based on rapid growth on media containing diesel oil as sole carbon source, the seven isolates were tested for biosurfactant production and emulsification activity. The obtained values of the surface tension measurements as well as the emulsification index (E24) are shown in table 3. The highest reduction of surface tension was achieved with B5 and A3 isolates with values of 25.3 mN m−1 and 28.1 mN m−1 respectively. The emulsifying capacity evaluated by the E24 emulsification index was highest in the culture of isolate B4 (78%), B5 (77%) and A3 (76%) as shown in table 3 and figure 2. These emulsions were stable even after 4 months. The bacteria with emulsification indices higher than 50 % and/or reduction in the surface tension (under 30 mN/m) have been defined as potential biosurfactant producers. Based on surface tension and the E24 index results, isolates B5, B4, A3 and A4 are the best candidates for biosurfactant production. It is important to note that, strains B4 and A4 produce biosurfactant in medium containing respectively 25% and 20% (w/v) NaCl.Stability of biosurfactant activities: The applicability of biosurfactants in several biotechnological fields depends on their stability at different environmental conditions (temperatures, pH and NaCl). For this study, the strain B4 appear very interesting (It can produce biosurfactant at 25 % NaCl) and was choosen for futher analysis for biosurfactant stability. The effects of temperature and pH on the biosurfactant production by the strain B4 are shown in figure 4.biosurfactant in medium containing respectively 25% and 20% (w/v) NaCl.Stability of biosurfactant activities: The applicability of biosurfactants in several biotechnological fields depends on their stability at different environmental conditions (temperatures, pH and NaCl). For this study, the strain B4 appear very interesting (It can produce biosurfactant at 25 % NaCl) and was chosen for further analysis for biosurfactant stability. The effects of temperature and pH on the biosurfactant production by the strain B4 are shown in figure 4. The biosurfactant produced by this strain was shown to be thermostable giving an E-24 Index value greater than 78% (figure 4A). Heating of the biosurfactant to 100 °C caused no significant effect on the biosurfactant performance. Therefore, the surface activity of the crude biosurfactant supernatant remained relatively stable to pH changes between pH 6 and 11. At pH 11, the value of E24 showed almost 76% activity, whereas below pH 6 the activity was decreased up to 40% (figure 4A). The decreases of the emulsification activity by decreasing the pH value from basic to an acidic region; may be due to partial precipitation of the biosurfactant. This result indicated that biosurfactant produced by strain B4 show higher stability at alkaline than in acidic conditions.Molecular identification and phylogenies of potential isolates: To identify halophilic bacterial isolates, the 16S rDNA gene was amplified using gene-specific primers. A PCR product of ≈ 1.3 kb was detected in all the seven isolates. The 16S rDNA amplicons of each bacterial isolate was sequenced on both strands using 27F and 1492R primers. The complete nucleotide sequence of 1336,1374, 1377,1313, 1305,1308 and 1273 bp sequences were obtained from A1, A2, A3, A4, B1, B4 and B5 isolates respectively, and subjected to BLAST analysis. The 16S rDNA sequence analysis showed that the isolated strains belong to the genera Halomonas, Staphylococcus, Salinivibrio, Planococcus and Halobacillus as shown in table 5. The halophilic isolates A2 and A4 showed 97% similarity with the Halomonas variabilis strain GSP3 (accession no. AY505527) and the Halomonas sp. M59 (accession no. AM229319), respectively. As for A1, it showed 96% similarity with the Halomonas venusta strain GSP24 (accession no. AY553074). B1 and B4 showed for their part 96% similarity with the Salinivibrio costicola subsp. alcaliphilus strain 18AG DSM4743 (accession no. NR_042255) and the Planococcus citreus (accession no. JX122551), respectively. The bacterial isolate B5 showed 98% sequence similarity with the Halobacillus trueperi (accession no. HG931926), As for A3, it showed only 95% similarity with the Staphylococcus arlettae (accession no. KR047785). The 16S rDNA nucleotide sequences of all the seven halophilic bacterial strains have been submitted to the NCBI GenBank database under the accession number presented in table 5. The phylogenetic association of the isolates is shown in figure 5.DICUSSIONThe physicochemical properties of the collected water samples indicated that this water was relatively neutral (pH 7.89) similar to the Dead Sea and the Great Salt Lake (USA) and in contrast to the more basic lakes such as Lake Wadi Natrun (Egypt) (pH 11) and El Golea Salt Lake (Algeria) (pH 9). The salinity of the sample was 224.70 g/L (22,47% (w/v). This range of salinity (20-30%) for Chott Tinsilt is comparable to a number of well characterized hypersaline ecosystems including both natural and man-made habitats, such as the Great Salt Lake (USA) and solar salterns of Puerto Rico. Thus, Chott Tinsilt is a hypersaline environment, i.e. environments with salt concentrations well above that of seawater. Chemical analysis of water sample indicated that Na +and Cl- were the most abundant ions, as in most hypersaline ecosystems (with some exceptions such as the Dead Sea). These chemical water characteristics were consistent with the previously reported data in other hypersaline ecosystems (DasSarma and Arora, 2001; Oren, 2002; Hacěne et al., 2004). Among 52 strains isolated from this Chott, seven distinct bacteria (A1, A2, A3, A4, B1, B4 and B5) were chosen for phenotypique, genotypique and phylogenetique characterization.The 16S rDNA sequence analysis showed that the isolated strains belong to the genera Halomonas, Staphylococcus, Salinivibrio, Planococcus and Halobacillus. Genera obtained in the present study are commonly occurring in various saline habitats across the globe. Staphylococci have the ability to grow in a wide range of salt concentrations (Graham and Wilkinson, 1992; Morikawa et al., 2009; Roohi et al., 2014). For example, in Pakistan, Staphylococcus strains were isolated from various salt samples during the study conducted by Roohi et al. (2014) and these results agreed with previous reports. Halomonas, halophilic and/or halotolerant Gram-negative bacteria are typically found in saline environments (Kim et al., 2013). The presence of Planococcus and Halobacillus has been reported in studies about hypersaline lakes; like La Sal del Rey (USA) (Phillips et al., 2012) and Great Salt Lake (Spring et al., 1996), respectively. The Salinivibrio costicola was a representative model for studies on osmoregulatory and other physiological mechanisms of moderately halophilic bacteria (Oren, 2006).However, it is interesting to note that all strains shared less than 98.7% identity (the usual species cut-off proposed by Yarza et al. (2014) with their closest phylogenetic relative, suggesting that they could be considered as new species. Phenotypic, genetic and phylogenetic analyses have been suggested for the complete identification of these strains. Theses bacterial strains were tested for the production of industrially important enzymes (Amylase, protease, lipase, DNAse and coagulase). These isolates are good candidates as sources of novel enzymes with biotechnological potential as they can be used in different industrial processes at high salt concentration (up to 25% NaCl for B4). Prominent amylase, lipase, protease and DNAase activities have been reported from different hypersaline environments across the globe; e.g., Spain (Sánchez‐Porro et al., 2003), Iran (Rohban et al., 2009), Tunisia (Baati et al., 2010) and India (Gupta et al., 2016). However, to the best of our knowledge, the coagulase activity has never been detected in extreme halophilic bacteria. Isolation and characterization of crude enzymes (especially coagulase) to investigate their properties and stability are in progress.The finding of novel enzymes with optimal activities at various ranges of salt concentrations is of great importance. Besides being intrinsically stable and active at high salt concentrations, halophilic and halotolerant enzymes offer great opportunities in biotechnological applications, such as environmental bioremediation (marine, oilfiel) and food processing. The bacterial isolates were also characterized for production of biosurfactants by oil-spread assay, measurement of surface tension and emulsification index (E24). There are few reports on biosurfactant producers in hypersaline environments and in recent years, there has been a greater increase in interest and importance in halophilic bacteria for biomolecules (Donio et al., 2013; Sarafin et al., 2014). Halophiles, which have a unique lipid composition, may have an important role to play as surface-active agents. The archae bacterial ether-linked phytanyl membrane lipid of the extremely halophilic bacteria has been shown to have surfactant properties (Post and Collins, 1982). Yakimov et al. (1995) reported the production of biosurfactant by a halotolerant Bacillus licheniformis strain BAS 50 which was able to produce a lipopeptide surfactant when cultured at salinities up to 13% NaCl. From solar salt, Halomonas sp. BS4 and Kocuria marina BS-15 were found to be able to produce biosurfactant when cultured at salinities of 8% and 10% NaCl respectively (Donio et al., 2013; Sarafin et al., 2014). In the present work, strains B4 and A4 produce biosurfactant in medium containing respectively 25% and 20% NaCl. To our knowledge, this is the first report on biosurfactant production by bacteria under such salt concentration. Biosurfactants have a wide variety of industrial and environmental applications (Akbari et al., 2018) but their applicability depends on their stability at different environmental conditions. The strain B4 which can produce biosurfactant at 25% NaCl showed good stability in alkaline pH and at a temperature range of 30°C-100°C. Due to the enormous utilization of biosurfactant in detergent manufacture the choice of alkaline biosurfactant is researched (Elazzazy et al., 2015). On the other hand, the interesting finding was the thermostability of the produced biosurfactant even after heat treatment (100°C for 30 min) which suggests the use of this biosurfactant in industries where heating is of a paramount importance (Khopade et al., 2012). To date, more attention has been focused on biosurfactant producing bacteria under extreme conditions for industrial and commercial usefulness. In fact, the biosurfactant produce by strain B4 have promising usefulness in pharmaceutical, cosmetics and food industries and for bioremediation in marine environment and Microbial enhanced oil recovery (MEOR) where the salinity, temperature and pH are high.CONCLUSIONThis is the first study on the culturable halophilic bacteria community inhabiting Chott Tinsilt in Eastern Algeria. Different genera of halotolerant bacteria with different phylogeneticaly characteristics have been isolated from this Chott. Culturing of bacteria and their molecular analysis provides an opportunity to have a wide range of cultured microorganisms from extreme habitats like hypersaline environments. Enzymes produced by halophilic bacteria show interesting properties like their ability to remain functional in extreme conditions, such as high temperatures, wide range of pH, and high salt concentrations. These enzymes have great economical potential in industrial, agricultural, chemical, pharmaceutical, and biotechnological applications. Thus, the halophiles isolated from Chott Tinsilt offer an important potential for application in microbial and enzyme biotechnology. In addition, these halo bacterial biosurfactants producers isolated from this Chott will help to develop more valuable eco-friendly products to the pharmacological and food industries and will be usefulness for bioremediation in marine environment and petroleum industry.ACKNOWLEDGMENTSOur thanks to Professor Abdelhamid Zoubir for proofreading the English composition of the present paper.CONFLICT OF INTERESTThe authors declare that they have no conflict of interest.Akbari, S., N. H. Abdurahman, R. M. Yunus, F. Fayaz and O. R. Alara, 2018. Biosurfactants—a new frontier for social and environmental safety: A mini review. Biotechnology research innovation, 2(1): 81-90.Association, A. P. H., A. W. W. Association, W. P. C. 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Roman biotechnol lett, 17(2): 7049-7064.Elazzazy, A. M., T. Abdelmoneim and O. Almaghrabi, 2015. Isolation and characterization of biosurfactant production under extreme environmental conditions by alkali-halo-thermophilic bacteria from Saudi Arabia. Saudi journal of biological Sciences, 22(4): 466-475.Graham, J. E. and B. Wilkinson, 1992. Staphylococcus aureus osmoregulation: Roles for choline, glycine betaine, proline, and taurine. Journal of bacteriology, 174(8): 2711-2716.Gupta, S., P. Sharma, K. Dev and A. Sourirajan, 2016. Halophilic bacteria of lunsu produce an array of industrially important enzymes with salt tolerant activity. Biochemistry research international, 1: 1-10.Gupta, S., P. Sharma, K. Dev, M. Srivastava and A. Sourirajan, 2015. A diverse group of halophilic bacteria exist in lunsu, a natural salt water body of Himachal Pradesh, India. SpringerPlus 4(1): 274.Hacěne, H., F. Rafa, N. Chebhouni, S. Boutaiba, T. Bhatnagar, J. C. Baratti and B. Ollivier, 2004. 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Connectivity metrics for surface water are important for predicting floods and droughts, and improving water management for human use and ecological integrity at the landscape scale. The integrated use of synthetic aperture radar (SAR) observations and geostatistics approach can be useful for developing and quantifying these metrics and their changes, including geostatistical connectivity function (GCF), maximum distance of connection (MDC), surface water extent (SWE), and connection frequency. In this study, we conducted a geostatistical analysis based on 52 wet and dry binary state (i.e., water and non-water) rasters derived from Sentinel-1 A/B GRD products acquired from 2015 to 2019 for China’s Momoge National Nature Reserve to investigate applicability and dynamics of the hydrologic connectivity metrics in an ungauged (i.e., data such as flow and water level are scarce) multi-lake system. We found: (1) generally, the change of GCF in North–South and Northeast–Southwest directions was greater than that in the West–East and Northwest–Southeast directions; (2) MDC had a threshold effect, generally at most 25 km along the W–E, NW–SE and NE–SW directions, and at most 45 km along the N–S direction; (3) the flow paths between lakes are diverse, including channelized flow, diffusive overbank flow, over-road flow and “fill-and-merge”; (4) generally, the values of the three surface hydrological connectivity indicators (i.e., the MDC, the SWE, and the conneciton frequency) all increased from May to August, and decreased from August to October; (5) generally, the closer the distance between the lakes, the greater the connection frequency, but it is also affected by the dam and road barrier. The study demonstrates the usefulness of the geostatistical method combining Sentinel-1 SAR image analysis in quantifying surface hydrological connectivity in an ungagged area. This approach should be applicable for other geographical regions, in order help resource managers and policymakers identify changes in surface hydrological connectivity, as well as address potential impacts of these changes on water resources for human use and/or ecological integrity at the landscape level.

Paleomagnetic measurements have been completed on 400 specimens from dated Archean granites and Matachewan dikes in the Michipicoten and Gamitagama greenstone belts in the western Wawa Subprovince of the Superior Province, Ontario. Detailed alternating-field and thermal step demagnetization analyses were used to isolate stable remanence directions. A single-component remanence was isolated within three adjacent dated granitic plutons on the eastern margin of the Michipicoten belt, including the Hawk Lake trondhjemite, the Southern external granite, and the Eastern external granite (HSE). The maximum possible age for this remanence is constrained by the intrusion of the last pluton at 2694 Ma. The corresponding HSE paleopole is located at 10°W, 41°S (dp = 8°, dm = 13°). A second paleopole, NB, is derived from the Northern external granite and the Baldhead River quartz monzonite, which give U–Pb zircon ages of 2662 and 2668 Ma, respectively. Their single-component remanence defines a paleopole on the Archean apparent polar wander path (APWP) at 15°E, 27°S (dp = 8°, dm = 13°), with a maximum possible age of 2.66 Ga. A third paleopole, GD, is derived from the north-northwest-trending Gamitagama diabase dikes and yields a position of 57°E, 41°N (dp = 7°, dm = 14°), which agrees with poles determined by other workers from the 2454 Ma Matachewan dike swarm. The GD pole, along with previously determined Matachewan dike poles, demonstrates that a tectonically stable craton has existed since intrusion of this extensive dike swarm, and it improves the precision of the 2454 Ma Matachewan pole on the APWP. These poles, when compared with coeval poles from the eastern side of the Kapuskasing Structural Zone in the Superior Province, imply no tectonic rotation or translation between the Wawa and Abitibi subprovinces along this Early Proterozoic structure.

The success story of a shale-gas reserve development in the United States is triggering a strong industry focus towards similar plays in Australia. The Cooper Basin (located at the border of South Australia and Queensland) and the Otway Basin (extending both onshore and offshore South Australia and Victoria) could be prime targets to develop shale-gas projects. The Cooper Basin, a late-Carboniferous to mid-Triassic basin, is the largest onshore sedimentary basin producing oil and gas from tight conventional reservoirs with low permeability. Fracture stimulation programs are used extensively to produce the oil and gas. Furthermore, new exploration strategies are now targeting possible commercial gas hosted in low-permeability Permian shale units. To maximise production, the development of shale-gas prospects requires a good understanding of the: 1. structure of the reservoirs; 2. mechanical properties of the stratigraphy; 3. fracture geometry and density; 4. in-situ stress field; and, 5. fracture stimulation strategies. In this study, we use a combination of seismic mapping techniques–including horizon and attribute mapping, and an analysis of wellbore geophysical logs–to best constrain the existing fracture network in the basins. This study is based on the processing and analysis of a 3D seismic cube–the Moomba Big Lake survey–which is located in the southwestern part of the Cooper Basin. This dataset covers an area encompassing both a structurally complex setting in the vicinity of a major fault to the SE of the survey, and an area of more subtle deformation corresponding to the southernmost part of the Nappamerri Trough. Structural fabrics trending ˜NW–SE and NE–SW, which are not visible on the amplitude seismic data, are revealed by the analysis of the seismic attributes–namely a similarity (equivalent to a coherency cube), dip steering and maximum curvature attributes. These orientations are similar to those of natural fractures mapped from borehole images logs, and can therefore be interpreted as imaging natural fractures across the Moomba-Big Lake area. This study is the first of its kind able to detect possible fractures from seismic data in the Cooper Basin. The methodology developed here can offer new insights into the structure of sedimentary basins and provide crucial parameters for the development of tight reservoirs. In parallel, a tentative forward model of the generation of a fracture network following a restoration of the Top Roseneath horizon was carried out. The relatively good correlation between the fracture orientations generated by the model and the fractures mapped from geophysical data shows that fractures in the Moomba-Big Lake area may have formed during either a N–S compressive principal horizontal stress, or an E–W compressive principal horizontal tectonic stress regime. In addition, the orientations of the fracture interpreted through this study are also compatible with a generation under the present day stress regime described in this part of the basin, with an maximal horizontal stress trending E–W.

OBJECTIVES/GOALS: Characterize formal informatics methods and approaches for enabling reproducible translational research. Education of reproducible methods to translational researchers and informaticians. METHODS/STUDY POPULATION: We performed a scoping review [1] of selected informatics literature (e.g. [2,3]) from PubMed and Scopus. In addition we reviewed literature and documentation of translational research informatics projects [4–21] at the University of Utah. RESULTS/ANTICIPATED RESULTS: The example informatics projects we identified in our literature covered a broad spectrum of translational research. These include research recruitment, research data requisition, study design and statistical analysis, biomedical vocabularies and metadata for data integration, data provenance and quality, and uncertainty. Elements impacting reproducibility of research include (1) Research Data: its semantics, quality, metadata and provenance; and (2) Research Processes: study conduct including activities and interventions undertaken, collections of biospecimens and data, and data integration. The informatics methods and approaches we identified as enablers of reproducibility include the use of templates, management of workflows and processes, scalable methods for managing data, metadata and semantics, appropriate software architectures and containerization, convergence methods and uncertainty quantification. In addition these methods need to be open and shareable and should be quantifiable to measure their ability to achieve reproducibility. DISCUSSION/SIGNIFICANCE OF IMPACT: The ability to collect large volumes of data collection has ballooned in nearly every area of science, while the ability to capturing research processes hasn’t kept with this pace. Potential for problematic research practices and irreproducible results are concerns.Reproducibility is a core essentially of translational research. Translational research informatics provides methods and means for enabling reproducibility and FAIRness [22] in translational research. In addition there is a need for translational informatics itself to be reproducible to make research reproducible so that methods developed for one study or biomedical domain can be applied elsewhere. Such informatics research and development requires a mindset for meta-research [23].The informatics methods we identified covers the spectrum of reproducibility (computational, empirical and statistical) and across different levels of reproducibility (reviewable, replicable, confirmable, auditable, and open or complete) [24–29]. While there are existing and ongoing efforts in developing informatics methods for translational research reproducibility in Utah and elsewhere, there is a need to further develop formal informatics methods and approaches: the Informatics of Research Reproducibility.In this presentation, we summarize the studies and literature we identified and discuss our key findings and gaps in informatics methods for research reproducibility. We conclude by discussing how we are covering these topics in a translational research informatics course.1.Pham MT, Rajić A, Greig JD, Sargeant JM, Papadopoulos A, McEwen SA. A scoping review of scoping reviews: advancing the approach and enhancing the consistency. Res Synth Methods. 2014 Dec;5(4):371–85.2.McIntosh LD, Juehne A, Vitale CRH, Liu X, Alcoser R, Lukas JC, Evanoff B. Repeat: a framework to assess empirical reproducibility in biomedical research. BMC Med Res Methodol [Internet]. 2017 Sep 18 [cited 2018 Nov 30];17. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5604503/3.Denaxas S, Direk K, Gonzalez-Izquierdo A, Pikoula M, Cakiroglu A, Moore J, Hemingway H, Smeeth L. Methods for enhancing the reproducibility of biomedical research findings using electronic health records. BioData Min. 2017;10:31.4.Burnett N, Gouripeddi R, Wen J, Mo P, Madsen R, Butcher R, Sward K, Facelli JC. Harmonization of Sensor Metadata and Measurements to Support Exposomic Research. In: 2016 International Society of Exposure Science [Internet]. Research Triangle Park, NC, USA; 2017 [cited 2017 Jun 17]. Available from: http://www.intlexposurescience.org/ISES20175.Butcher R, Gouripeddi RK, Madsen R, Mo P, LaSalle B. CCTS Biomedical Informatics Core Research Data Service. In Salt Lake City; 2016.6.Cummins M, Gouripeddi R, Facelli J. A low-cost, low-barrier clinical trials registry to support effective recruitment. In Salt Lake City, Utah, USA; 2016 [cited 2018 Nov 30]. Available from: //campusguides.lib.utah.edu/UtahRR16/abstracts7.Gouripeddi R, Warner P, Madsen R, Mo P, Burnett N, Wen J, Lund A, Butcher R, Cummins MR, Facelli J, Sward K. An Infrastructure for Reproducibile Exposomic Research. In: Research Reproducibility 2016 [Internet]. Salt Lake City, Utah, USA; 2016 [cited 2018 Nov 30]. Available from: //campusguides.lib.utah.edu/UtahRR16/abstracts8.Eilbeck K, Lewis SE, Mungall CJ, Yandell M, Stein L, Durbin R, Ashburner M. The Sequence Ontology: a tool for the unification of genome annotations. Genome Biol. 2005;6:R44.9.Gouripeddi R, Cummins M, Madsen R, LaSalle B, Redd AM, Presson AP, Ye X, Facelli JC, Green T, Harper S. Streamlining study design and statistical analysis for quality improvement and research reproducibility. J Clin Transl Sci. 2017 Sep;1(S1):18–9.10.Gouripeddi R, Eilbeck K, Cummins M, Sward K, LaSalle B, Peterson K, Madsen R, Warner P, Dere W, Facelli JC. A Conceptual Architecture for Reproducible On-demand Data Integration for Complex Diseases. In: Research Reproducibility 2016 (UtahRR16) [Internet]. Salt Lake City, Utah, USA; 2016 [cited 2017 Apr 25]. Available from: https://zenodo.org/record/16806711.Gouripeddi R, Lane E, Madsen R, Butcher R, LaSalle B, Sward K, Fritz J, Facelli JC, Cummins M, Shao J, Singleton R. Towards a scalable informatics platform for enhancing accrual into clinical research studies. J Clin Transl Sci. 2017 Sep;1(S1):20–20.12.Gouripeddi R, Deka R, Reese T, Butcher R, Martin B, Talbert J, LaSalle B, Facelli J, Brixner D. Reproducibility of Electronic Health Record Research Data Requests. In Washington, DC, USA; 2018 [cited 2018 Apr 21]. Available from: https://zenodo.org/record/1226602#.WtvvyZch27013.Gouripeddi R, Mo P, Madsen R, Warner P, Butcher R, Wen J, Shao J, Burnett N, Rajan NS, LaSalle B, Facelli JC. A Framework for Metadata Management and Automated Discovery for Heterogeneous Data Integration. In: 2016 BD2K All Hands Meeting [Internet]. Bethesda, MD; November 29-30 [cited 2017 Apr 25]. Available from: https://zenodo.org/record/16788514.Groat D, Gouripeddi R, Lin YK, Dere W, Murray M, Madsen R, Gestaland P, Facelli J. Identification of High-Level Formalisms that Support Translational Research Reproducibility. In: Research Reproducibility 2018 [Internet]. Salt Lake City, Utah, USA; 2018 [cited 2018 Oct 30]. Available from: //campusguides.lib.utah.edu/UtahRR18/abstracts15.Huser V, Kahn MG, Brown JS, Gouripeddi R. Methods for examining data quality in healthcare integrated data repositories. Pac Symp Biocomput Pac Symp Biocomput. 2018;23:628–33.16.Lund A, Gouripeddi R, Burnett N, Tran L-T, Mo P, Madsen R, Cummins M, Sward K, Facelli J. Enabling Reproducible Computational Modeling: The Utah PRISMS Ecosystem. In Salt Lake City, Utah, USA; 2018 [cited 2018 Oct 30]. Available from: //campusguides.lib.utah.edu/UtahRR18/abstracts17.Pflieger LT, Mason CC, Facelli JC. Uncertainty quantification in breast cancer risk prediction models using self-reported family health history. J Clin Transl Sci. 2017 Feb;1(1):53–9.18.Shao J, Gouripeddi R, Facelli J. Improving Clinical Trial Research Reproducibility using Reproducible Informatics Methods. In Salt Lake City, Utah, USA; 2018 [cited 2018 Oct 30]. Available from: //campusguides.lib.utah.edu/UtahRR18/abstracts19.Shao J, Gouripeddi R, Facelli JC. Semantic characterization of clinical trial descriptions from ClincalTrials.gov and patient notes from MIMIC-III. J Clin Transl Sci. 2017 Sep;1(S1):12–12.20.Tiase V, Gouripeddi R, Burnett N, Butcher R, Mo P, Cummins M, Sward K. Advancing Study Metadata Models to Support an Exposomic Informatics Infrastructure. In Ottawa, Canada; 2018 [cited 2018 Oct 30]. Available from: = http://www.eiseverywhere.com/ehome/294696/638649/?&t=8c531cecd4bb0a5efc6a0045f5bec0c321.Wen J, Gouripeddi R, Facelli JC. 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The research on wind regimes and the wind protection mechanism of sand-fixing plants has mainly relied on wind tunnel experiments; few observations have been made in the field. At the same time, airflow around individual standing vegetation elements and communities is relatively lacking in alpine semi-arid deserts. Therefore, this paper selected 10-year-old Hippophae rhamnoides (sea buckthorn) on sandy land on the eastern shore of Qinghai Lake as the study object. Based on spatial and temporal changes of wind regime in the afforestation forest, a structural simulation of airflow near the plant and at different layers above the ground, and the annual changes in wind protection, we studied the wind protection mechanisms of H. rhamnoides as single elements or communities. The results were as follows: the effective protection length of the sublayer of H. rhamnoides was 1.0 to 1.8 m. The higher the layer, the smaller the decrease in wind velocity behind elements, and the smaller the effective protection length. Wind velocity downwind of H. rhamnoides increased, with height increasing where the airflow decreases rate (R) decreased in the sublayer, and increasing in the middle layer as plant height increased. Meanwhile, the airflow decreases rate (R) was negative in the upper layer because it decreased as the plant height increased. The airflow movement between elements had various directions because the upper layer was prone to fluctuations due to the swinging of the crown and branches, and turbulence was seen at the sublayers owing to the mechanical resistance of the elements. When the wind speed at the standard point was 8.5 m/s and the wind direction was east (E), the increase of airflow velocity at the side and center in the upper layer was more significant, and there was a strong wind zone in the azimuth of NW‒N‒NE‒E‒SE, while the S‒SW‒W azimuth zone was weaker. The sand-fixing shrub H. rhamnoides had a significant windproof function, and the 1.5 m square interval density of H. rhamnoides was suitable for alpine desert control projects.

Abstract Seismicity in the southeastern United States is relatively poorly characterized and thus not well understood. Structures and heterogeneities from multiple stages of Appalachian orogenesis, continental rifting, and magmatism as well as rivers and reservoirs may be influencing seismic activity in the region, but previous constraints are limited. The addition of seismic stations from the U.S. Transportable Array and the Southeastern Suture of the Appalachian Margin Experiment Array in 2012–2014 provide an opportunity to characterize seismicity in the central Georgia–South Carolina region. We develop a seismic catalog of &gt;1000 events from March 2012 to May 2014 within or near the instrument array boundaries 30.1°–35.2°N, 80.9°– 85.7°W. Many of the events detected were industrial blasts, so multiple strategies were tested to discriminate between earthquakes and blasts based on event locations, timing, and spectral amplitude of the P and S arrivals. Based on this analysis, ∼10% of the events in the catalog were classified as earthquakes. Most earthquakes southeast of the eastern Tennessee seismic zone are located in the Carolina terrane, particularly where the Carolina terrane intersects major rivers or reservoirs. One prominent region of seismicity along the Savannah River near Thurmond Lake corresponds with an ∼4.5 m rise in water levels in 2013. A temporal cluster of earthquakes in April 2013 was followed by increased levels of ambient seismicity preceding the nearby Mw 4.1 earthquake in 2014. Focal mechanisms based on first-motion polarities indicate strike-slip to oblique-thrust motion on structures trending approximately north–south or east–west, and a maximum horizontal stress orientation consistent with the regional trend of ∼N60°E, implying that seismicity may reactivate more optimally oriented structures in the Carolina terrane that are oblique to the trend of the Appalachians. Seismicity in central Georgia appears to be controlled by a complex interaction between preexisting crustal structure and hydrologic variability.

During an intense survey of natural woodlands around Lake Hodges (33°N, 117°W) in June 2001, symptoms typical of root and collar rot caused by Phytophthora spp. were observed on 27% of 474 coast live oaks (Quercus agrifolia Nee.) and on none of 86 Engelmann oaks (Q. engelmannii Greene), in spite of complete spatial intermixing of the two species. Symptoms on coast live oaks included viscous exudates emerging through intact bark matched by underbark dark lesions with irregular margins. Lesions were delineated by a dark line and present on the root collar or the buttress of symptomatic trees. Crowns of trees with lesions ranged from completely healthy to declining or dead. All symptomatic trees were in proximity of the lake or streams. Phytophthora cinnamomi Rands was isolated from four trees in three distinct sites by plating tissues from lesion margins on PARP selective medium and from four soil samples by using standard pear baiting and plating lesions from pear tissue onto PARP. Identification of the isolates was obtained from microscopic observations and direct sequencing of the internal transcribed spacer region of the rDNA (Genbank Accession Nos. AY302148, MC2 and AY302149, MC3). P. citricola Sawada was also isolated once. Pathogenicity tests were completed to compare the susceptibility of the two species of oaks growing in the Lake Hodges region with P. cinnamomi. Two P. cinnamomi isolates from Lake Hodges (MC2, ATCC MYA-3711; MC3) and one isolate from an avocado orchard in San Diego County (MC6) were used to inoculate separately 10 5-year-old trees each of Q. agrifolia and Q. engelmannii grown in 5-gallon containers. Inoculations were performed in two lath-house experiments during February and September 2002 by placing an 8-mm diameter V8-agar plug from the margin of a P. cinnamomi colony underbark and sealing the wound with Parafilm and grafting wax. Lesion lengths were measured 2 months after inoculation, and the presence of the pathogen confirmed by reisolation on PARP. Mean average, maximum, and minimum temperatures were 14, 19, and 9°C and 21, 24, and 18°C for the February and September inoculations, respectively. The February inoculation resulted in small lesions only on Q. agrifolia (26 ± 15 mm, SD). The September inoculation resulted in 135 ± 68 mm (SD) lesions on Q. agrifolia and 49 ± 35 mm (SD) lesions on Q. engelmannii. Controls did not show any lesions. The length of lesions was significantly different between the two hosts (P < 0.0001) and significant differences were observed among the three isolates (P = 0.0018). Although Q. agrifolia is a known host for P. cinnamomi in California (2,3), to our knowledge, this is the first report of widespread infestation of P. cinnamomi in natural oak woodlands in the western United States. Survey and inoculation results indicated Q. engelmannii to be less susceptible to infection. Inoculation results confirm previous research that cold temperatures are unfavorable to this pathogen and isolates differed in pathogenicity toward Q. agrifolia. Decline of oaks infected by P. cinnamomi was observed only in conjunction with other factors, in particular with the presence of the oak twig girdler, Agrilus angelicus Horn., an insect favored by stress conditions such as drought. Similar effects have been reported for Mediterranean oaks infected by the same pathogen (1). References: (1) C. M. Brasier. Nature 360:539, 1992. (2) P. A. Miller. Western Shade Tree Conf. Proc. 8:39, 1941. (3) S. M. Mircetich et al. Plant Dis. Rep. 61:66, 1977.

Wrench-fault structures below Danian limestone and Palaeogene marl, and an overlying structural framework of Quaternary glacial deposits in north-east Sjælland, Denmark, are interpreted from two vibro-seismic sections recorded to 600 msec TWT depth. The main seismic section is 6.3 km long, N–S oriented, and intersected by a 0.7 km long, E–W oriented satellite seismic section. In addition, boreholes in the vicinity of the seismic profile are used for the interpretation. The sections were acquired in 2014 along the western shoreline of the lake Esrum Sø in the Gribskov area. In the lower part of the seismic section (the interval 100–300 msec TWT), parallel-bedded geological layers occur along most of the profile apart from six locations, where six wrench-fault structures displace the upper part of the Chalk Group and lower Palaeogene marl. The northernmost of the six wrench-fault locations correlates to the eastern slope of the buried Esrum–Alnarp valley, which suggests that the valley is an inherited tectonic feature. The location of the wrench- fault structures supports the outline of faults related to the Sorgenfrei-Tornquist Zone on previous geological maps, which had almost no seismic data from the area. Above the stratigraphic level presented by the Danian limestone and lower Palaeogene marl, a composite glaciotectonic complex comprising two glaciodynamic sequences is recognized by e.g. thrust-fault structures and the lithostratigraphy of glacial successions recorded in the wells. In parts of the seismic sections, the lowermost level of the glaciotectonic complex inherited the wrench-tectonic fault structures, most significantly seen in the northern segment. The advance of the Scandinavian ice sheet caused the glaciotectonic structures displayed in the seismic section. The two sequences represent events related to the Norwegian and the Swedish glacial advances. From the interpretation of the seismic section it is found that the glaciotectonic complex conceals the wrench-tectonic flower structures.

ABSTRACT: Teacher performance has been the focus of educational policy reforms in recent decades for the professional development of teachers. The purpose of this study was to determine the effect of teaching experience and peer teaching skills on basic training on ECE teacher performance. This research uses ex-post facto quantitative method of comparative analysis and design by level. The population is all ECE teachers who attend basic-level education and training in Tangerang Regency, totaling 3358 people consisting of 116 male teachers and 3,242 female teachers. Data collection techniques using a questionnaire with data analysis include descriptive analysis. Requirements test analysis and inferential analysis. The results show that there are differences in the performance of ECE teachers between teachers with more than five years of teaching experience and less than five years, in the group of ECE teachers with high peer teaching skills and low peer teaching skills. 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This study aim to attempt mapping out the Land Use or Land Cover (LULC) status of Regional Project Coordination Committee (RPCC) between 2009-2019 with a view of detecting the land consumption rate and the changes that has taken place using RS and GIS techniques; serving as a precursor to the further study on urban induced variations or change in weather pattern of the cityn Rangpur City Corporation(RCC) is the main administrative functional area for both of Rangpur City and Rangpur division and experiencing a rapid changes in the field of urban sprawl, cultural and physical landscape,city growth. These agents of Land use or Land cover (LULC) varieties are responsible for multi-dimensional problems such as traffic congestion, waterlogging, and solid waste disposal, loss of agricultural land. In this regard, this study fulfills LULC changes by using Geographical Information Systems (GIS) and Remote Sensing (RS) as well as field survey was conducted for the measurement of change detection. The sources of data were Landsat 7 ETM and landsat 8 OLI/TIRS of both C1 level 1. Then after correcting the data, geometrically and radiometrically change detection and combined classification (supervised & unsupervised) were used. The study finds LULC changes built-up area, water source, agricultural land, bare soil in a change of percentage is 17.23, 2.58, -9.94, -10.19 respectively between 2009 and 2019. Among these changes, bare soil is changed to a great extent, which indicates the expansion of urban areas is utilizing the land to a proper extent. Keywords: Urban expansion; land use; land cover; remote sensing; geographic information system (GIS); Rangpur City Corporation(RCC). References Al Rifat, S. A., & Liu, W. (2019). 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(2008). Impact of land use and land cover change on the water balance of a large agricultural watershed: Historical effects and future directions. Water Resources Research, 44(7). doi:10.1029/2007wr006644 Copyright (c) 2019 Geosfera Indonesia Journal and Department of Geography Education, University of Jember This work is licensed under a Creative Commons Attribution-Share A like 4.0 International License

Abstract. The Alpine mountains in central Europe are characterized by a heterogeneous crust accumulating different tectonic units and blocks in close proximity to sedimentary foreland basins. Centroid moment tensor inversion provides insight into the faulting mechanisms of earthquakes and related tectonic processes but is significantly aggravated in such an environment. Thanks to the dense AlpArray seismic network and our flexible bootstrap-based inversion tool Grond, we are able to test different setups with respect to the uncertainties of the obtained moment tensors and centroid locations. We evaluate the influence of frequency bands, azimuthal gaps, input data types, and distance ranges and study the occurrence and reliability of non-double-couple (DC) components. We infer that for most earthquakes (Mw≥3.3) a combination of time domain full waveforms and frequency domain amplitude spectra in a frequency band of 0.02–0.07 Hz is suitable. Relying on the results of our methodological tests, we perform deviatoric moment tensor (MT) inversions for events with Mw>3.0. Here, we present 75 solutions for earthquakes between January 2016 and December 2019 and analyze our results in the seismotectonic context of historical earthquakes, seismic activity of the last 3 decades, and GNSS deformation data. We study regions of comparably high seismic activity during the last decades, namely the Western Alps, the region around Lake Garda, and the eastern Southern Alps, as well as clusters further from the study region, i.e., in the northern Dinarides and the Apennines. Seismicity is particularly low in the Eastern Alps and in parts of the Central Alps. We apply a clustering algorithm to focal mechanisms, considering additional mechanisms from existing catalogs. Related to the N–S compressional regime, E–W-to-ENE–WSW-striking thrust faulting is mainly observed in the Friuli area in the eastern Southern Alps. Strike-slip faulting with a similarly oriented pressure axis is observed along the northern margin of the Central Alps and in the northern Dinarides. NW–SE-striking normal faulting is observed in the NW Alps, showing a similar strike direction to normal faulting earthquakes in the Apennines. Both our centroid depths and hypocentral depths in existing catalogs indicate that Alpine seismicity is predominantly very shallow; about 80 % of the studied events have depths shallower than 10 km.

The early age is a substantial period in human development. The long-term benefit from participating in the early childhood education programs is increasing opportunities for the children to complete their higher education in order to get the better welfare future. In Sumedang regency - the location of this study - the children’s participations in joining the early childhood education programs had not been optimized yet. Approximately 47% of the children aged 4-6 years had not participated in the early childhood education programs yet. Thus, it was significant to seek out the factors influencing the children’s accessibilities to participate in the early childhood education programs in Sumedang regency in order to find out the alternative policy to increase their accessibilities to participate in the early childhoodeducation programs. The research was conducted in 2017 using the quantitative method. The data were obtained from the “2015 Susenas” which involved 300 respondents. The data analysis was performed by using the logistic regression with the 12th version of STATA software. The findings showed that the children’s accessibilities to participate in the early childhood education programs in Sumedang regency was determined by the educational background of the head of family, the marital status of the parents, the head of the family’s income, the number of the family members, and the children’s domicile. Giving knowledge about the importance of early childhood education to the lower educational background parents and enlarging the early childhood schools’ facilities and infrastructures especially in rural areas of Sumedang regency still needed to be improved in order to have better human resources in the future. References Abdulhak, I. (2003). Memposisikan pendidikan anak usia dini dalam sistem pendidikan nasional. Buletin PADU Jurnal Ilmiah Anak Dini Usia. Jakarta: Dir. PAUD, Dirjend. PLSP, Depdiknas. Apriana, R. (2009). 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RESUMENEl objetivo de este artículo es reflexionar sobre los mecanismos de financiación y de control de las instituciones religiosas por los laicos en las primeras décadas de la conquista y colonización de Hispanoamérica. Investigar sobre la inversión laica en lo sagrado supone en un primer lugar aclarar la historiografía sobre laicos, religión y dinero en las sociedades de Antiguo Régimen y su trasposición en América, planteando una mirada desde el punto de vista de las motivaciones múltiples de los actores seglares. A través del ejemplo de restituciones, donaciones y legados en losAndes, se explora el papel de los laicos españoles, y también de las poblaciones indígenas, en el establecimiento de la densa red de instituciones católicas que se construye entonces. La propuesta postula el protagonismo de actores laicos en la construcción de un espacio cristiano en los Andes peruanos en el siglo XVI y principios del XVII, donde la inversión económica permite contribuir a la transición de una sociedad de guerra y conquista a una sociedad corporativa pacificada.PALABRAS CLAVE: Hispanoamérica-Andes, religión, economía, encomienda, siglos XVI y XVII.ABSTRACTThis article aims to reflect on the mechanisms of financing and control of religious institutions by the laity in the first decades of the conquest and colonization of Spanish America. Investigating lay investment in the sacred sphere means first of all to clarifying historiography on laity, religion and money within Ancien Régime societies and their transposition to America, taking into account the multiple motivations of secular actors. The example of restitutions, donations and legacies inthe Andes enables us to explore the role of the Spanish laity and indigenous populations in the establishment of the dense network of Catholic institutions that was established during this period. The proposal postulates the role of lay actors in the construction of a Christian space in the Peruvian Andes in the sixteenth and early seventeenth centuries, when economic investment contributed to the transition from a society of war and conquest to a pacified, corporate society.KEY WORDS: Hispanic America-Andes, religion, economics, encomienda, 16th and 17th centuries. BIBLIOGRAFIAAbercrombie, T., “Tributes to Bad Conscience: Charity, Restitution, and Inheritance in Cacique and Encomendero Testaments of 16th-Century Charcas”, en Kellogg, S. y Restall, M. 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White pine blister rust, caused by Cronartium ribicola J.C. Fisch., was found on southwestern white pine (Pinus flexilis James var. reflexa Engelm., synonym P. strobiformis Engelm.) near Hawley Lake, Arizona (Apache County, White Mountains, 34.024°N, 109.776°W, elevation 2,357 m) in April 2009. Although white pines in the Southwest (Arizona and New Mexico) have been repeatedly surveyed for blister rust since its discovery in the Sacramento Mountains of southern New Mexico in 1990 (1,2), this was the first confirmation of C. ribicola in Arizona. Numerous blister rust cankers were sporulating on 15- to 30-year-old white pines growing in a mixed conifer stand adjacent to a meadow with orange gooseberry bushes (Ribes pinetorum Greene), a common telial host in New Mexico. Most of the observed cankers were producing their first aecia on 5-year-old branch interwhorl segments (i.e., formed in 2004). The two oldest cankers apparently originated on stemwood formed about 14 and 21 years before (1995 and 1988). Neither uredinia nor telia were seen on expanding gooseberry leaves in late April, but these rust structures were found later in the season. Voucher specimens deposited in the Forest Pathology Herbarium-Fort Collins (FPF) were determined by host taxa and macro- and microscopic morphology as C. ribicola–white pine with typical cankers, aecia, and aeciospores (1). Six collections of aeciospores from single, unopened aecia provided rDNA sequences (ITS1-5.8S-ITS2, primers ITS1F and ITS4) with two different repeat types (GenBank Accession Nos. HM156043 and HM156044 [J. W. Hanna conducted analysis with methods described in 3]). A BLASTn search with these sequences showed 100 and 99% similarities, respectively, with sequences of C. ribicola, including accessions L76496, L76498, and L76499 from California (4). Additional reconnaissance of white pines on the Fort Apache Indian Reservation and neighboring Apache-Sitgreaves National Forests was conducted from May through September 2009. Although the blister rust infestation was distributed over more than 100 km2 of forest type, infected trees were restricted to mesic and wet canyon bottoms (climatically high-hazard sites) and were not found on dry sites–even where aecial and telial hosts occurred together. Recent dispersal within the White Mountains was suggested by a presence of infected gooseberry plants on several sites where infected white pines were not yet evident. Geils et al. (1) concluded that the initial infestation in New Mexico had originated by long-distance, aerial transport from California to the Sacramento Mountains in 1969. Since then, numerous additional infestations in the Southwest have been discovered; but we do not know which of these (including Arizona) resulted by dispersal from California or New Mexico. Although rust may eventually infest many host populations in the Southwest and disease may kill most trees in some locations, differences in site hazard and spread provide managers with numerous opportunities to maintain white pines and Ribes spp. References: (1) B. Geils et al. For. Pathol. 40:147, 2010. (2) F. Hawksworth. Plant Dis. 74:938, 1990. (3) M.-S. Kim et al. For. Pathol. 36:145, 2006. (4) D. Vogler and T. Bruns. Mycologia 90:244, 1998.

Scoria cones are abundant in most volcanic fields on Earth, such as the Michoacán-Guanajuato Volcanic Field, in the central-western sector of the Trans-Mexican Volcanic Belt. However, there are few in-depth studies on their eruptive style and controlling factors, despite of their diversity in shape and composition which implies a wide range of hazards. Here, we present results of morphologic, stratigraphic, sedimentary, petrographic, and geochemical studies of the prominent Las Cabras scoria cone located west of the Zacapu lacustrine basin in the center of the Michoacán-Guanajuato Volcanic Field. This basaltic andesitic to andesitic volcano formed between 27 and 26 kyrs BP on the steep slopes (>10º) of the lava shield of El Tule volcano. Over time, its dominant eruptive style changed from Strombolian to effusive. Initial explosive activity built a 170-m-high scoria cone and deposited thick tephra fallout on the surrounding sloping terrain. Structures in the deposits indicate that early friable fine-grained tephra underwent significant erosion due to syn-eruptive heavy rain coupled with the sloping nature of the underlying ground. This erosion generated lahars that very likely reached the Zacapu lake based on the pre-eruptive topography. As the explosivity dropped, lava was emitted from the base of the cone first to the S and SE, forming a thick, viscous lobe that filled a pre-existing E-W valley. The flow direction then deviated to the N and NE, to form thinner, less-viscous lobes fed from the vent by an open-channel. The lavas are covered by hummocks made of agglutinates and bombs that indicate that the eruption terminated by catastrophic collapse of the SE sector of the cone, possibly triggered by the intrusion of magma within the cone, which destabilized its downslope segment. The sudden flank failure was potentially associated with a late effusive event and the hummocks may have been carried away by the lava surge. Whole-rock chemical variations and crystal disequilibrium textures point toward a complex magma feeding system, involving mixing and mingling between different magma batches. This study shows that the formation of scoria cones on a terrain with a marked slope (>10°) has profound impacts on the eruption dynamics and related hazards due to its effect on cone stability and ash erosion. It also evidences the erosive effect of syn-eruptive rain on fine-grained tephra, especially when deposited on a slope. Finally, it reveals the complex magmatic processes that may occur in the shallow plumbing system of monogenetic andesitic volcanoes, which could be particularly important in inland areas of continental arcs.

Introduction The prevalence of acute mountain sickness (AMS) is increasing with altitude (i.e., 10-25% at 2,500 m and 50-85% at ~ 5,000 m; Bärtsch & Swenson, 2013). While there is no error-free test to predict its occurrence, several risk factors and tests have been proposed. For example, the hypoxic ventilatory response (HVR) measures the ratio between the increase in ventilation (VE) and the decrease in pulse saturation (SpO2) during hypoxic exposure. Some studies reported an increased (Lhuissier et al., 2012), no difference (Pokorski and Marczak, 2003), or a decreased HVR (Kronenberg and Drage, 1973) with age. The effect of sex remains also debated since women have been reported to have a higher (Richalet et al., 2012) or lower (Schneider et al. 2002; Vardy et al., 2006) AMS prevalence. Therefore, we aimed to compare measurements of HVR, VE and SpO2 between pre- (PreM) and post-menopausal (postM) women and to investigate if they are related to AMS. We hypothesized differences in hypoxic tolerance between age groups. Methods We screened pre-menopausal women (PreM; n = 13; age = 31.7 ± 7.8yr; weight = 63.5 ±9.6 kg; height = 167 ±10 cm) during three phases (early follicular, Fol1; late follicular, Fol2; luteal, Lut3) of their menstrual cycle and post-menopausal women (PostM; n = 15; age = 62.8 ±2.3 yr; weight = 56.1 ±8.3 kg; height 163 ±5 cm) on one occasion. They were evaluated with a pure nitrogen breathing test (N2T; Solaiman et al., 2014) for HVR and with a cycling exercise (5 min of rest followed by 5 min of cycling at 1.5 W/kg) in hypoxia (FiO2 = 14%; simulated altitude of 3,500 m) with measurement of SpO2 and VE. They were then exposed to one night in real altitude (3,375 m) with AMS assessment (Lake Louise Score; Roach et al., 2018). Results PreM had a higher resting VE in normoxia (9.95-10.07 vs 8.50 L/min; P < 0.05) and increased VE (7.49-8.78 vs 5.41 L/min; P < 0.05) during the N2T at the three measurements points than PostM. Moreover, only at Fol2, HVR (-0.43 vs -0.27 L/min/%; P = 0.023), VEpeak (18.9 vs 15.0 L/min; P = 0.025) during N2T and resting SpO2 in normoxia (95.9 vs 94.9, P = 0.093) were higher in PreM. The prevalence of AMS was similar between PreM and PostM (30.8 vs 40.0%). When AMS positive and AMS negative subgroups were compared, no difference in HVR was found while there were differences in SpO2 and VE. Discussion/Conclusion The main finding of the present study is that HVR was higher in PreM than in PostM only during the late follicular phase of the former. Since estrogen is known to have a stimulatory effect on both pulmonary ventilation and blood vessel vasodilation and peaks during this phase, this suggests that it is the main trigger of the observed differences in HVR. The prevalence of AMS was in line with the literature for a similar altitude (34% at 3,650m; Maggiorini et al., 1990). Contrary to Richalet et al. (2012), HVR did not diagnose AMS in any group nor was lower in the older age group. No other parameter showed to be a solid predictive metric for AMS. Given conflicting results in this study (i.e., HVR and AMS) and in the literature, there is no clear evidence of an effect of age on hypoxic tolerance and on AMS prediction. References Bärtsch, P., & Swenson, E. R. (2013). Acute high-altitude illnesses. New England Journal of Medicine, 368(24), 2294-2302. https://doi.org/10.1056/NEJMcp1214870 Kronenberg, R. S., & Drage, C. W. (1973). Attenuation of the ventilatory and heart rate responses to hypoxia and hypercapnia with aging in normal men. The Journal of Clinical Investigation, 52(8), 1812-1819. https://doi.org/10.1172/JCI107363 Lhuissier, F. J., Canouï‐Poitrine, F., & Richalet, J. P. (2012). Ageing and cardiorespiratory response to hypoxia. The Journal of Physiology, 590(21), 5461-5474. https://doi.org/10.1113/jphysiol.2012.238527 Maggiorini, M., Bühler, B., Walter, M., & Oelz, O. (1990). Prevalence of acute mountain sickness in the Swiss Alps. British Medical Journal, 301(6756), 853-855. https://doi.org/10.1136/bmj.301.6756.853 Pokorski, M., & Marczak, M. (2003). Ventilatory response to hypoxia in elderly women. Annals of Human Biology, 30(1), 53-64. https://doi.org/10.1080/03014460210162000 Richalet, J. P., Larmignat, P., Poitrine, E., Letournel, M., & Canouï-Poitrine, F. (2012). Physiological risk factors for severe high-altitude illness: A prospective cohort study. American Journal of Respiratory and Critical Care Medicine, 185(2), 192-198. https://doi.org/10.1164/rccm.201108-1396OC Roach, R. C., Hackett, P. H., Oelz, O., Bärtsch, P., Luks, A. M., MacInnis, M. J., ... & Lake Louise AMS Score Consensus Committee. (2018). The 2018 Lake Louise acute mountain sickness score. High Altitude Medicine & Biology, 19(1), 4-6. https://doi.org/10.1089/ham.2017.0164 Schneider, M., Bernasch, D., Weymann, J., Holle, R., & Bärtsch, P. (2002). Acute mountain sickness: influence of susceptibility, preexposure, and ascent rate. Medicine & Science in Sports & Exercise, 34(12), 1886-1891. Solaiman, A. Z., Feehan, R. P., Chabitnoy, A. M., Leuenberger, U. A., & Monahan, K. D. (2014). Ventilatory responses to chemoreflex stimulation are not enhanced by angiotensin II in healthy humans. Autonomic Neuroscience, 183, 72-79. https://doi.org/10.1016/j.autneu.2014.01.010 Vardy, J., Vardy, J., & Judge, K. (2006). Acute mountain sickness and ascent rates in trekkers above 2500 m in the Nepali Himalaya. Aviation, Space, and Environmental Medicine, 77(7), 742-744.

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Ruang esensinya adalah tempat manusia hidup dan beraktivitas. Namun tidak semua aktivitas dapat terakomodir karena setiap ruang dibatasi dengan fungsinya masing-masing. Bagi ruang pribadi keterbatasan ruang tersebut merupakan karakteristik utama bagi ruang itu sendiri sedangkan pada ruang publik yang memiliki berbagai macam aktivitas harus dapat menampung berbagai aktivitas di dalamnya. Untuk itulah perlu dilakukan kajian mengenai ruang publik terhadap permasalahan keterbatasan ruang yang sering ditemui. Tulisan ini menggunakan metode kualitatif dengan melakukan pendekatan kajian literatur. Ada beberapa literatur yang diambil dari beberapa ahli serta beberapa hasil penelitian dalam artikel jurnal untuk mendukung teori dan melihat kenyataan di lapangan. Pada akhir tulisan diambil suatu kesimpulan bahwa keterbatasan ruang publik terhadap berbagai macam aktivitas yang berlangsung di dalamnya dengan terbentuknya ruang bersama baik secara permanen maupun bergantian (waktu tertentu).Kata-kata Kunci: arsitektur, ruang publik, aktivitas, ruang bersamaURBAN PUBLIC SPACE STUDIES BETWEEN ACTIVITIES AND LIMITATIONSThe essence of space is a place where humans live and doing their activities. But not all activities can be accommodated because space is limited by their functions. For private space, space limitations are the main characteristics for space itself, while in public spaces that have various kinds of activities must be able to accommodate multiple activities in it. For this reason, a study of public space needs to be done on the problems of space limitations that are often encountered. This paper uses qualitative methods by conducting a literature review approach. There is some literature taken from several experts and several research results in the journal for support the theory and see the reality in the field. At the end of the writing, it was concluded that the limitations of the public space for various kinds of activities take place in it with the formation of shared spaces both permanently and alternately (certain times).Keywords: architecture, public space, activity, share spaceREFERENCESAgustapraja, H. R. (2018). Studi Pemetaan Perilaku (Behavioral Mapping) Pejalan Kaki Pada Pedesterian Alun-Alun Kota Lamongan. Civilla, 3(1), 134–139. https://doi.org/https://doi.org/10.30736/cvl.v3i1.220Athanassiou, E. (2017). The Hybrid Landscape Of Public Space In Thessaloniki In The Context Of Crisis. Landscape Research, 42(7), 782–794. https://doi.org/10.1080/01426397.2017.1372399Carr, J., & Dionisio, M. R. (2017). Flexible Spaces as a Third Way Forward for Planning Urban Shared Spaces. In Cities (pp. 73–82). 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Nalars, 17(1), 21–28. https://doi.org/10.24853/nalars.17.1.21-28Salomon-Ayeh, B. E., King, R. S., & Decardi-Nelson, I. (2011). Street Vending and The Use of Urban Public Space in Kumasi, Ghana. Surveyor, 4(1), 20–31. Retrieved from http://dspace.knust.edu.gh/bitstream/123456789/3423/1/Surveyor Journal 3.pdfSantoso, J. T., Mustikawati, T., Suryasari, N., & Titisari, E. Y. (2016). Pola Aktivitas Wisata Belanja dI Kampung Wisata Keramik Dinoyo, Malang. Tesa Arsitektur, 14(1), 1. https://doi.org/10.24167/tes.v14i1.560Simpson, P. (2011). Street Performance And The City: Public Space, Sociality, And Intervening In The Everyday. Space and Culture, XX(X), 1–16. https://doi.org/10.1177/1206331211412270Sudarisman, I. (2017). Kajian Pedagang Kaki Lima Di Taman Tegalega, Bandung, Jawa Barat. Arsir , 1(2), 161–174. Retrieved from http://jurnal.um-palembang.ac.id/arsir/article/view/867/769

DRIP IRRIGATION WETTING PATTERNS IN A MID FLORIDA SANDY SOIL JOSÉ ALVES JÚNIOR1; WIJE MALLIKAARACHCHIGE BANDARANAYAKE2 E JAMES SYVERTSEN2 1Eng. Agr. Professor Adjunto da Escola de Agronomia (EA) da Universidade Federal de Goiás (UFG), Avenida Esperança, s/n, Campus Samambaia, Goiânia, Goiás, Brasil, CEP: 74.690-900. jose.junior@pq.cnpq.br2Professor and Researcher in University of Florida, IFAS, CREC, Lake Alfred-FL 33850, USA. wijeb@ufl.edu, jmsn@ufl.edu 1 ABSTRACT The wetting pattern of soil under drip irrigation is governed by soil texture, structure, initial water content, emitter spacing, discharge rate and irrigation frequency. Although drip irrigation is not common in central Florida “Ridge soils”, the “advanced citrus production system” can hold a promising future. High frequency, short duration pulses is an important factor to consider for efficient irrigation in this very sandy “Ridge” soils. The objective of this research was to evaluate the water distribution pattern under 1.9, 4.6 and 8.6 L h-1 discharge rate drippers over different wetting durations using three evaluation methods: a) EC5 soil water sensors, b) blue dye tracer, and c) a simple mathematical model developed for sandy soils. Of the three methods, the dye method is labor intensive and time consuming and was used only to evaluate one pulse rate with 3 pulse durations. Soil water sensors, though relatively expensive, gave the most detailed wetting patterns under different combinations of pulse rates and wetting durations. The model overestimated the wetting depth and underestimated the wetting diameter. With the initial soil moisture at 0.055 m3 m-3 and a constant application pressure of 138 kPa, the maximum achievable wetting diameter with increasing pulse rate and pulse duration was limited to 0.75 m. However, the wetting depth increased >1 m with >4.63 L h-1 pulse rate and 3-h duration. Preferential flow was minimal but had little influence on wetting pattern. The best combination was the 4.63 L h-1 emitter with a 3 h pulse that yielded the maximum wetting diameter (0.77 m) and the optimum wetting depth (0.77 m). Keywords: Trickle irrigation, soil water sensors, FD&CC blue dye, soil water movement, water use efficiency. ALVES JÚNIOR., J.; BANDARANAYAKE, W. M.; SYVERTSEN, J. BULBO MOLHADO SOB IRRIGAÇÃO POR GOTEJAMENTO EM SOLO ARENOSO NA FLORIDA 2 RESUMO O padrão de umedecimento do solo sob irrigação por gotejamento é regido pela textura do solo, a estrutura, o conteúdo inicial de água solo, espaçamento entre emissores, a vazão do emissor e freqüência de irrigação. Embora, a irrigação por gotejamento não seja comum no centro da Flórida, no "sistema avançado de produção de citros" pode conter um futuro promissor. Alta freqüência, pulsos de curta duração é um fator importante a considerar para irrigação eficiente neste solo arenoso. O objetivo deste trabalho foi avaliar o padrão de distribuição de água em diferentes vazões de gotejadores 1,9, 4,6 e 8,6 L h-1 ao longo de diferentes períodos de molhamento usando três métodos de avaliação : a) sensores de água no solo EC5 , b) corante azul , e c) um modelo matemático simples. Dos três métodos, o método do corante é trabalhoso e consome muito tempo, e por isso só foi utilizado para avaliar uma vazão em 3 diferentes tempos de irrigação. Sensores de água no solo, apesar de relativamente caro, deu os padrões de molhamento mais detalhados sob diferentes combinações de vazão e tempo de irrigação. O modelo superestimou a profundidade molhada e subestimou o diâmetro molhado. Com a umidade do solo inicial de 0,055 m3 m-3 e uma pressão de aplicação constante de 138 kPa, o diâmetro molhado máximo obtido entre as vazões e tempos de irrigação avaliados foi de 0,75 m. No entanto, profundidade molhada ultrapassa 1 m, com 4,63 L h-1 de vazão do gotejador e 3 h de tempo de irrigação. Fluxo preferencial foi mínimo, mas teve pouca influência sobre o bulbo molhado. A melhor combinação foi a vazão de 4,63 Lh-1 com um tempo de 3 h, que produziu o diâmetro máximo de molhagem (0,77 m) e a profundidade de molhagem óptima (0,77 m). Palavras-Chave: Irrigação localizada; sensors de água no solo; FD&CC blue dye; movimento de água no solo

Climate change induced sea-level rise (SLR) is on its increase globally. Regionally the lowlands of China, Vietnam, Bangladesh, and islands of the Malaysian, Indonesian and Philippine archipelagos are among the world’s most threatened regions. Sea-level rise has major impacts on the ecosystems and society. It threatens coastal populations, economic activities, and fragile ecosystems as mangroves, coastal salt-marches and wetlands. This paper provides a summary of the current state of knowledge of sea level-rise and its effects on both human and natural ecosystems. The focus is on coastal urban areas and low lying deltas in South-East Asia and Vietnam, as one of the most threatened areas in the world. About 3 mm per year reflects the growing consensus on the average SLR worldwide. The trend speeds up during recent decades. The figures are subject to local, temporal and methodological variation. In Vietnam the average values of 3.3 mm per year during the 1993-2014 period are above the worldwide average. Although a basic conceptual understanding exists that the increasing global frequency of the strongest tropical cyclones is related with the increasing temperature and SLR, this relationship is insufficiently understood. Moreover the precise, complex environmental, economic, social, and health impacts are currently unclear. SLR, storms and changing precipitation patterns increase flood risks, in particular in urban areas. Part of the current scientific debate is on how urban agglomeration can be made more resilient to flood risks. Where originally mainly technical interventions dominated this discussion, it becomes increasingly clear that proactive special planning, flood defense, flood risk mitigation, flood preparation, and flood recovery are important, but costly instruments. Next to the main focus on SLR and its effects on resilience, the paper reviews main SLR associated impacts: Floods and inundation, salinization, shoreline change, and effects on mangroves and wetlands. The hazards of SLR related floods increase fastest in urban areas. This is related with both the increasing surface major cities are expected to occupy during the decades to come and the increasing coastal population. In particular Asia and its megacities in the southern part of the continent are increasingly at risk. The discussion points to complexity, inter-disciplinarity, and the related uncertainty, as core characteristics. An integrated combination of mitigation, adaptation and resilience measures is currently considered as the most indicated way to resist SLR today and in the near future.References Aerts J.C.J.H., Hassan A., Savenije H.H.G., Khan M.F., 2000. Using GIS tools and rapid assessment techniques for determining salt intrusion: Stream a river basin management instrument. 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The quantity of municipal solid waste (MSW) generation is escalating at an alarming rate with every passing year alongside the modernization of our economy. Unfortunately, the majority of this waste remains uncollected or ends up in open dumping and followed by uncontrolled burning. Citing the deep-rooted consequences, open dumping should be absolutely abandoned and scientific interventions should be aggressively exercised to reclaim the municipal brownfields. The present research work undertook the judicial task of assessing the comparative feasibility of biomining and scientific capping as a technology selection for reclamation of about a decade old 120 million tons of waste chunk laying at Jawahar Nagar dump yard. Primary dump samples were collected from various locations, considering depth as a variable. While leachate and groundwater samples were collected from Malkaram lake and preinstalled borewells receptively. Additionally, the ambient air quality and noise level also been ascertained within the buffer zone. The blended representative solid sample was segregated using a 70 mm mesh size trommel into organic and inorganic fractions. The organic fraction was composted using a lab-scale aerobic static pile composting (ASPC) while the trommel reject was processed as refuse derived fuel (RDF). Evidently, the compost lagged quality and depicted nutrient deficiency. While the burning of RDF produced siloxane gas, significantly due to elevated silicon level in the primary waste. Furthermore, due to the prolonged leaching tenure and seasonal dilution, the concentration of legacy leachate was relatively weaker. Borewell samples collected from a depth of 20 feet also portrayed minor contamination up to 500 meters horizontal radius. The issue of leachability can solely be resolved with the capping of the existing dump and the end product quality derived from the biomining process is highly questionable. Thus, handling such large quantity capping is a befitting option over biomining for Jawahar Nagar dumpsite. INTRODUCTION Presently, in India due to rapid urbanization and industrialization, the generation of MSW has been increasing tremendously and also expected to continue a similar trend in the future (Scott, 1995; Bhat et al., 2017; Sethurajan et al., 2018; Sharma et al., 2018). Annually, the comprehensive urban MSW generation in India is more than 62 million tons. Metro cities are the mammoth contributor of the entire chunk and waste production had already reached an alarming figure of 50,000 tonnes/day. While the waste generation from the tier 2 cities is also rigorously escalating and presently contribute up to 20,000 tones/day (Sharma et al., 2018). A study conducted by the central pollution control board (CPCB) revealed MSW generation in India is increasing at a distressing rate of 5 % per annum with a sharp escalation in the quantities of domestic hazardous waste (Sharma et al., 2018). With major financial constraints, inefficacy of collection, treatment, and disposal incurs further reasons to worry. So far India has miserably failed to set up wholesome source segregation and collection method. Presently, the country spends more than 60% of its annual waste management budget only in collection. Besides, only 20% or less of the collected materials are scientifically handled and treated. Citing the statistics, it is evident that the majority of the MSW is simply gets dumped on the low laying grounds located somewhere on the outskirts of the cities. The precipitation, infiltration, surface water runoff, bird menace, rodent interference etc. triggers the vulnerability of waste and leads to mal odor, ground and surface water contamination, human and environmental health deterioration (Jayawardhana et al., 2016). Further, the perseverance of the inorganic and inert fractions leads to soil contamination, poses a fire threat, and also may incur carcinogenicity and acute toxicity among the animals (Mir et al., 2021). There are numerous techniques for the reclamation and remediation of the dumpsites, includes processes such as capping and closure, in-situ vitrification, sub-surface cut-off walls, and waste biomining (Chakrabarti and Dubey, 2015; Thakare and Nandi, 2016). Waste biomining is a stable way to get rid of the entire range of problems associated with open dumping and reclaim valuable land (Kaksonen et al., 2017). There are several instances including reclamation of Mumbai Gorai dump yard by IL & FS Environment, 70 – 80 years old 12,00,000 tons of dump clearance by Nagar Nigam Indore within a minute span of 3 years and many more. But the process of biomining is highly sensitive and case-specific. The success of the process solely depends on factors such as characteristics of the waste, efficacy of the effective microorganism culture, acceptability of the processed end product at the local market etc. (Jerez, 2017; Banerjee et al., 2017; Venkiteela, 2020). Contrarily, though the scientific capping is not an end-to-end solution but still advisable in the cases where the quantity of waste is gigantic, land scarcity is prevalent, no nearby industries to consume the end products etc. Mehta et al. (2018) have also supported the above claim based on the assessment of locations specific MSW dump reclamation case studies. While in another Nagpur-based case study conducted by Ashootosh et al. (2020) reported the superiority of the biominingprocess over simple land capping due to the favorability of the local conditions. Capping eliminates the environmental interference and thereby reduces biosphere contamination and leachate generation. Further, it captivates rodent and vector breeding and thereby curtails the spreading of communicable diseases and improves aesthetics. But right consolidation through compaction and execution is utmost necessary in the above case. As non-compaction and faulty sloping will easily lead to heavy settlement and slope failure (Berkun et al., 2005; Al-Ghouti et al., 2021). The present study has been pursued with the primary objective to run a techno-commercial assessment between scientific capping and biomining. While the secondary objective was to ascertain the level of contamination and propose mitigative measures. MATERIALS AND METHODStudy Area Spanning over 350 acres of a precious piece of land at the outskirts of Hyderabad city, Jawahar Nagar dumping yard was brutally utilized by the Greater Hyderabad Municipal Corporation (GHMC) for open dumping for a prolonged tenure of 10 years. It housed nearly 12 lakh metric tons of heterogeneous solid and domestic hazardous waste and continues polluting until 2015, until the Ramky group was offered to cap the legacy dumping and scientifically handle the site. The present study has been facilitated at Hyderabad Municipal Solid Waste Limited, formerly known as Jawahar Nagar dump yard to analyze and assess the feasibility of bio-mining as handling and management alternate to the existing practice of scientific capping. The epicenter of processing and disposal facility is lying approximately on the cross-section of 17°31'24.45"N and 78°35'23.37"E. As per the contract, the comprehensive legacy dumping to be capped in three phases over about 150 acres of area and Ramky has significantly entered the phase two of the operation only within a span of five years by successfully capping more than half of the legacy footprint. Sampling Methodology The waste pile was divided into three layers namely, base, middle, and top. A uniform amount of sample was collected from the successive layers of all five different corners which cover north, south, east, west, and central of the garbage pile. Sampling inspections were performed using a manual auger besides large samples were collected using a JCB excavator. The top six-inch layer of the pile was removed to avoid any contamination while collecting the samples and 5-10 kg of sample was collected from each of the locations. Further, intermediate and bottom layer samples were collected by digging a 500 mm diameter hole through the heap. A composite was prepared by a homogenized blending of all the fifteen grub samples. The blend was distributed into four equal quadrants and the top and bottom quadrants were eliminated diagonally while the left-over quadrants were mixed thoroughly. This process was repeated until a sample of the required bulk of 20 kg is obtained. Surface and subsurface water samples from borewell were collected in and around the facility. Piezometric monitoring borewells located near the landfills were utilized for the subsurface sample collection. While a rainwater pond turned leachate lake named Malkaram was determined as the primary source for leachate collection. Buffer samples were collected from Ambedkar Nagar, the nearby colony exiting at a distance of only 300 meters. Lab-scale Experimentation The representative sample was characterized for composition and further screened through a 70 mm mesh size trommel. The trommel permeate was considered as the organic fraction while the reject was mostly inorganics and inert. The organics were subjected to ASPC. The quantity of the air required is arrived using the method delineated below (Figure 1). MSW Pile size: 2m x 0.5m x 0.5m Volume of pile: 0.5 m3 Average Density of MSW: 620 Kg/m3 Weight of pile: 310 Kg Nitrogen required for matured compost: 9300 mg/kg dry : 9300 X 310 mg : 2.88 x 106 mg : 2.88 Kg Total air required: 2.88 x 100/76 [as Nitrogen in air is 76% by weight] : 3.79 Kg of dry air : 3.79/1.225 m3 [@ 15 deg C density of air 1.225 kg/m3] : 3.1 m3 This air is to be supplied for 100 min / day for 0.5 m pile Air flow rate required: 3.1 x 60/100 = 1.86 m3/h (for practical purpose a flowrate of 2 m3/h was maintained). The maturation period was considered as 28 days and post-maturation, the stabilized material was further cured for 24 hours and screened using 12 mm and 4 mm trommel respectively to obtain the desired product quality and particle size. Whereas, the trommel reject was evenly spreader on the copper trays and dried in an oven at 1050C for 2 hours. The dried material was micronized to the size of 50 mm or below using a scissor and inert such as glass, sand, stone etc. were segregated manually (Mohan and Joseph, 2020). Concurrently, a bench-scale capped landfill prototype was built using the below-mentioned procedure to evaluate the factors such as settlement and slope stability. A 30 mm thick low permeable soil was laid on the top of the waste, followed by a 60 mm layer of compacted clay liner (CCL). Each join between successive liner material was closely monitored. A 1.5 mm thick HDPE liner was placed on the top of the CCL. A 285 GSM geotextile membrane was placed as the successive above layer followed by a 15 mm thick drainage media layer. A further layer of geotextile membrane was placed on top of the drainage media for better stabilization, grip, and strength. The top vegetative soil layer of 45 mm thickness was laid off on top of the geotextile media and St. Augustine grass was rooted (Cortellazzo et al., 2020; Ashford et al., 2000). 2.4 Sample Analysis pH, Electrical Conductivity (EC) and Turbidity of the samples were analyzed using pH, EC-TDS, and Nephelometer of Mettler Toledo. The pH meter was calibrated with the buffer solution of 4.0, 7.0 & 9.12 at a controlled temperature. EC-TDS meter was calibrated with 0.1 M KCL having 12.8 mS/cm of conductivity. Nephelometer was calibrated with Formazine solution of 10 & 100 NTU. Total Dissolved Solids (TDS), (mg/L) was performed using the gravimetric method at 1800C in the oven. Titrimetric parameters such as Total Alkalinity as CaCO3 (mg/L), Total Hardness as CaCO3 (mg/L), Chloride as Cl- (mg/L), Calcium as Ca2+ (mg/L), Residual Free Chlorine (RFC), (mg/L) were analyzed using APHA (American Public Health Associations) method, 23rd Edition, 2017. Total Kjeldahl Nitrogen (mg/L) and Ammonical Nitrogen (mg/L) were performed through distillation followed by titration with H2SO4 as a titrant. Sulphide as S2- was done with the Iodometric method after distillation. Each titrimetric parameter was analyzed in triplicate after standardizing the titrant with required reagents and crossed checked by keeping a check standard. Sodium as Na (mg/L) and Potassium as K (mg/L) were performed using Flame Photometer. The photometer was calibrated with different standards from 10 to 100 (mg/L) standard solutions. The leachate sample was diluted enough to get the value within the standard range and cross-checked with check standards at the same time. Chemical Oxygen Demand (COD), (mg/L) was performed using the open reflux method for 2 hours at 1500C in COD Digestor. Biochemical Oxygen Demand (BOD), (mg/L) was performed using the alkali iodide azide method for 3 days. The samples were kept in a BOD incubator at 270C for 3 days. It was kept in duplicate to have a check on quality control. Sulphate was analyzed by the gravimetric method instead of turbidimetric or through UV-Visible spectrophotometer as its concentration was found more than 40 mg/L. Nitrate as NO3- was analyzed after filtration at 220-275 nm, while Hexavalent Chromium as Cr6+ was analyzed at 540 nm in the UV-Vis. Parameters like Cyanide as CN-, Fluoride as F-, and Phenolic Compounds were gone through a distillation process followed by UV-Vis. The distillation process ensures the removal of interferences presents either positive or negative. For the parameters like Total Iron or Ferric Iron, the samples were digested properly with the required reagents on the hot plate before analyzing in UV-Vis. For the metal analysis the water samples were digested at a temperature of 1000C using aqua regia as a media. The samples were digested to one-fourth of the volume on a hot plate. The recommended wavelengths as per APHA 3120 B were selected for each of the metals. The standard graph was plotted for each of the metals before analysis and crossed checked with the check standard at the same time. Parameters such as bulk density and particle size were performed through the certified beaker and sieve. The percentage of moisture content was estimated using the oven by keeping the compost sample for 2 hours at 1050C. C/N ratio was estimated through CHNS analyzer keeping sulfanilamide as a check standard. The analysis was performed by extracting the desired component in the desired solution prescribed in the method followed by converting the same from mg/L to mg/Kg. RESULTS AND DISCUSSION An exhaustive bench-study has been pursued and real-time samples were collected and analyzed for all possible parameters to determine the pros and cons attributed to both processes. The investigation begins by collecting the samples and concluded by impact assessment studies inclusive of the buffer zone. Both solid, liquid, and gaseous samples were precisely investigated to opt for the best solution. A detailed finding of the investigation is summarized below. Primarily, the representative solid sample was characterized through a manual separation process and the results are portrayed in Figure 1. Compost Characterization ASPC of the organic fraction has resulted in a recovery of 46.7% of the initial load. While 53.3% of the influent mass were inert and barely degradable fraction contributes to reject, the rest 4.1% is miscellaneous process loss. The processed compost was extensively analyzed including for metal contamination and the same is tabulated in Table 1. The value of C/N ratio, OC, TN, K2O, P2O5, and NPK evidently portrays the shortcoming in terms of nutrient availability. Though it is highly enriched in organic carbon and thus the same can be effectively utilized as a soil preconditioner. Ayilara et al. (2020) also reported a similar finding, where the city compost sourced from MSW lagged major plant nutrients. RDF Characterization Processed trommel rejects constitute cloth, rexine, leather, jute, paper, plastics, coir and other inert contributed to RDF. The fraction of inert was as high as 37.2% of the overall RDF mass and it mostly constituted glass and sand. The combined weight of sand and glass fragments contributed 73.5% of the total inert, while the rest was stone and small brickbats. The higher level of silicon associated with the presence of glass and sand yielded siloxane and triggered the possibility of kiln corrosion. A detailed RDF analysis report is enclosed in Table 2. The values explicitly portray the quality of RDF is moderately lower and higher salts concentration is extremely prevalent. With relatively lower NCV and such high salt concentration, the above specimen will certainly pose a corrosion threat to the kiln and shall be either neglected as kiln feed or can be utilized after dilution with Grade III RDF quality. Further, such high ash generation will also induct high transportation and landfill charges. Leachate Characterization The Malkaram leachate lake is the end result of prolonged, slow, and steady mixing of the legacy leachate through the existing fissure cracks in the sheath rock bottom profile. Apparently, the concentration of leachate is significantly lower due to the dilution. Samples were analyzed in triplicates and the mean value is tabulated here in Table 3. The metal concertation and rest of the parameter values are well within the secondary treatment influent range, except for TDS. Thus, a modular aerobic biological treatment unit such as moving bed biofilm bioreactor (MBBR) or membrane bioreactor (MBR) would be a well-suited pick. However, a reverse osmosis (RO) system needs to be installed to get rid of the high TDS content. The permeate of RO can be reused back into the system. Whereas, the reject can be converted into dried powder through forced evaporation mechanisms. The higher concentration of salts in RDF collaterally justifies the elevated TDS level in leachate. In a leachate impact assessment study performed by El-Salam and Abu-Zuid (2015) the reported BOD/COD ratio of 0.69 is greater than double the value of 0.301 reported in Table 3. Though the difference in both the values are quite high, it is relatable and justifiable by the huge age difference of the source waste. The primarily characterized data is of a fresh leachate generated from regular MSW, while the later one is from a decade old waste that barely has any unstabilized organic content. Groundwater Contamination The obvious reason for downward leachate infiltration and osmotic movement facilitates groundwater contamination. Both surface and subsurface water samples were collected within the dump yard and the buffer zone and analyzed using the standard methods. The results are portrayed in Table 4. The slightly alkaline pH of the borewell sample is an indication of the ongoing anaerobic process. The dissolved oxygen value of 3.5 mg/L further validates the correlation. Higher TDS and hardness values are self-indicative of elevated salt concentration in source waste. Eventually, the same interfered with the RDF quality. Positively in the case of all the parameters, a successive decrement in pollution concentration has been spotted from dump ground towards the buffer zone. In a similar study conducted by Singh et al. (2016) at Varanasi, Uttar Pradesh the reported concentration of the parameters is significantly higher than reported in Table 4. The basic reason behind variation is the dissimilarities of the local soil profile. The sandy and clay loam soil profile of Varanasi allows a greater rate of percolation and infiltration. While the bottom sheath rock profile at Jawahar Nagar permits the only a minute to little percolation rate. The difference in percolation rate is directly correlated to the concentration levels in this case. Contrarily, Kurakalva et al. (2016) have reported much-elevated pollutant concertation both in ground and surface water for a study conducted at the same site in 2016. The higher concentration is relatable to the fact of the non-closure of the open dump back then. Capping activity had at Jawahar Nagar gained its pace 2018 onwards and capping for the primary section of 70 acres got concluded only during mid of 2019. Due to the decrement in runoff and percolation, the quality of both surface and subsurface water has improved drastically. Impact Assessment The odor and groundwater contamination are two of the primary issues that triggered a massive public agitation initially. The root causes of both the issues are identified as rainwater percolation and anaerobic digestion respectively. Eventually, the completion of the capping process would resolve both the problems effectively. Other non-tangential impacts include nausea; headache; irritation of the eye, nasal cavity, and throat; diarrhoeal diseases; vector-borne disease, cattle toxicity etc. Scientific capping can easily cater as the wholesome solution for all (Cortellazzo et al., 2020). Yu et al. (2018) had performed an extensive study to comprehend the relativity of respiratory sickness and MSW borne air pollution. The study made a couple of dreadful revelations such as gases released due to the anaerobic digestion of MSW such as methane, hydrogen sulphide, and ammonia incur detrimental impact on Lysozyme and secretory immunoglobulin A (SIgA). While SO2 was reported as the lung capacity and functionality reducer. Further, a gender-specific study executed by the same research group revealed, air pollution impacts more severely on male children than the female and retards immune functions. Presently, the area of 351 acres has been developed as Asia’s one of the largest state of the art municipal solid waste processing and disposal facility by Ramky Enviro Engineers Limited. This ensured zero dumping and no further environmental interventions. As legal compliance, the facility monitors the quality of groundwater and ambient air quality in and around the facility on monthly basis to assure the biosafety. The variation in concentration of various monitoring parameters between 2012 to 2020 is summarized in Figure 2. The concentration of each of the parameters are showcased in ppm and a standard equipment error was settled at 3% for respirable dust sampler and multi-gas analyzer (Taheri et al., 2014). Despite all parameter values have gradually increased except for methane, the facility still managed to maintain them well under the regulatory limits. The decrement in methane concentration is directly correlated to the practice of aerobic composting and aeration-based secondary treatment that prevented the formation of the anaerobic atmosphere and henceforth methane generation. While for the rest of the parameters the increment in values is quite substantial and predictable due to the sudden escalation in MSW generation in the past decade in correlation with Gross domestic product (GDP) enhancement. The observed and interpreted impacts due to the elevated pollutant level are in-line with the georeferenced findings reported by Deshmukh and Aher (2016) based on a study conducted at Sangamner, Maharashtra. CONCLUSION The study critically analyzed and investigated every techno-environmental and socio-economic aspect correlated to open dumping. The bench-scale experimentation revealed the efficiency of the single liner scientific capping is fair enough to eliminate any further rainwater infiltration, however, it has no control over the generation of leachate due to the inherent moisture. Internal moisture related issue was anyhow compensated with pertinent compaction prior to dispose of the waste. Contrarily, both the products derived through the biomining process namely, compost and RDF lagged quality due to scantier nutrient content and higher salt and silicon content respectively. Besides, impact assessment studies concede the pollutant concentration in groundwater in and around the plant has drastically diminished post-July 2019 due to the partial completion of waste capping. It also abetted lowering the dust and odor issues relatively in the surrounding. ACKNOWLEDGMENT The authors would like to sincerely acknowledge GHMC, Hyderabad Integrated Municipal Solid Waste Limited, and Ramky Enviro Engineers Limited for enabling us to pursue the sample collection and other necessary onsite activities. Further, the authors would like to register profound acknowledgment to EPTRI for supporting us with the essential experimental facilities. REFERENCES Sharma, A., Gupta, A.K., Ganguly, R. (2018), Impact of open dumping of municipal solid waste on soil properties in mountainous region. 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We’ll have trouble now!The Archaeological Society of Jutland was founded on Sunday, 11 March 1951. As with most projects with which P.V Glob was involved, this did not pass off without drama. Museum people and amateur archaeologists in large numbers appeared at the Museum of Natural History in Aarhus, which had placed rooms at our disposal. The notable dentist Holger Friis, the uncrowned king of Hjørring, was present, as was Dr Balslev from Aidt, Mr and Mrs Overgaard from Holstebro Museum, and the temperamental leader of Aalborg Historical Museum, Peter Riismøller, with a number of his disciples. The staff of the newly-founded Prehistoric Museum functioned as the hosts, except that one of them was missing: the instigator of the whole enterprise, Mr Glob. As the time for the meeting approached, a cold sweat broke out on the foreheads of the people present. Finally, just one minute before the meeting was to start, he arrived and mounted the platform. Everything then went as expected. An executive committee was elected after some discussion, laws were passed, and then suddenly Glob vanished again, only to materialise later in the museum, where he confided to us that his family, which included four children, had been enlarged by a daughter.That’s how the society was founded, and there is not much to add about this. However, a few words concerning the background of the society and its place in a larger context may be appropriate. A small piece of museum history is about to be unfolded.The story begins at the National Museum in the years immediately after World War II, at a time when the German occupation and its incidents were still terribly fresh in everyone’s memory. Therkel Mathiassen was managing what was then called the First Department, which covered the prehistoric periods.Although not sparkling with humour, he was a reliable and benevolent person. Number two in the order of precedence was Hans Christian Broholm, a more colourful personality – awesome as he walked down the corridors, with his massive proportions and a voice that sounded like thunder when nothing seemed to be going his way, as quite often seemed to be the case. Glob, a relatively new museum keeper, was also quite loud at times – his hot-blooded artist’s nature manifested itself in peculiar ways, but his straight forward appearance made him popular with both the older and the younger generations. His somewhat younger colleague C.J. Becker was a scholar to his fingertips, and he sometimes acted as a welcome counterbalance to Glob. At the bottom of the hierarchy was the student group, to which I belonged. The older students handled various tasks, including periodic excavations. This was paid work, and although the salary was by no means princely, it did keep us alive. Student grants were non-existent at the time. Four of us made up a team: Olfert Voss, Mogens Ørsnes, Georg Kunwald and myself. Like young people in general, we were highly discontented with the way our profession was being run by its ”ruling” members, and we were full of ideas for improvement, some of which have later been – or are being – introduced.At the top of our wish list was a central register, of which Voss was the strongest advocate. During the well over one hundred years that archaeology had existed as a professional discipline, the number of artefacts had grown to enormous amounts. The picture was even worse if the collections of the provincial museums were taken into consideration. We imagined how it all could be registered in a card index and categorised according to groups to facilitate access to references in any particular situation. Electronic data processing was still unheard of in those days, but since the introduction of computers, such a comprehensive record has become more feasible.We were also sceptical of the excavation techniques used at the time – they were basically adequate, but they badly needed tightening up. As I mentioned before, we were often working in the field, and not just doing minor jobs but also more important tasks, so we had every opportunity to try out our ideas. Kunwald was the driving force in this respect, working with details, using sections – then a novelty – and proceeding as he did with a thoroughness that even his fellow students found a bit exaggerated at times, although we agreed with his principles. Therkel Mathiassen moaned that we youngsters were too expensive, but he put up with our excesses and so must have found us somewhat valuable. Very valuable indeed to everyon e was Ejnar Dyggve’s excavation of the Jelling mounds in the early 1940s. From a Danish point of view, it was way ahead of its time.Therkel Mathiassen justly complained about the economic situation of the National Museum. Following the German occupation, the country was impoverished and very little money was available for archaeological research: the total sum available for the year 1949 was 20,000 DKK, which corresponded to the annual income of a wealthy man, and was of course absolutely inadequate. Of course our small debating society wanted this sum to be increased, and for once we didn’t leave it at the theoretical level.Voss was lucky enough to know a member of the Folketing (parliament), and a party leader at that. He was brought into the picture, and between us we came up with a plan. An article was written – ”Preserve your heritage” (a quotation from Johannes V. Jensen’s Denmark Song) – which was sent to the newspaper Information. It was published, and with a little help on our part the rest of the media, including radio, picked up the story.We informed our superiors only at the last minute, when everything was arranged. They were taken by surprise but played their parts well, as expected, and everything went according to plan. The result was a considerable increase in excavation funds the following year.It should be added that our reform plans included the conduct of exhibitions. We found the traditional way of presenting the artefacts lined up in rows and series dull and outdated. However, we were not able to experiment within this field.Our visions expressed the natural collision with the established ways that comes with every new generation – almost as a law of nature, but most strongly when the time is ripe. And this was just after the war, when communication with foreign colleagues, having been discontinued for some years, was slowly picking up again. The Archaeological Society of Jutland was also a part of all this, so let us turn to what Hans Christian Andersen somewhat provocatively calls the ”main country”.Until 1949, only the University of Copenhagen provided a degree in prehistoric archaeology. However, in this year, the University of Aarhus founded a chair of archaeology, mainly at the instigation of the Lord Mayor, Svend Unmack Larsen, who was very in terested in archaeology. Glob applied for the position and obtained it, which encompassed responsibility for the old Aarhus Museum or, as it was to be renamed, the Prehistoric Museum (now Moesgaard Museum).These were landmark events to Glob – and to me, as it turned out. We had been working together for a number of years on the excavation of Galgebakken (”Callows Hill”) near Slots Bjergby, Glob as the excavation leader, and I as his assistant. He now offered me the job of museum curator at his new institution. This was somewhat surprising as I had not yet finished my education. The idea was that I was to finish my studies in remote Jutland – a plan that had to be given up rather quickly, though, for reasons which I will describe in the following. At the same time, Gunner Lange-Kornbak – also hand-picked from the National Museum – took up his office as a conservation officer.The three of us made up the permanent museum staff, quickly supplemented by Geoffrey Bibby, who turned out to be an invaluable colleague. He was English and had been stationed in the Faeroe Islands during the war, where he learned to speak Danish. After 1945 he worked for some years for an oil company in the Gulf of Persia, but after marrying Vibeke, he settled in her home town of Aarhus. As his academic background had involved prehistoric cultures he wanted to collaborate with the museum, which Glob readily permitted.This small initial flock governed by Glob was not permitted to indulge inidleness. Glob was a dynamic character, full of good and not so good ideas, but also possessing a good grasp of what was actually practicable. The boring but necessary daily work on the home front was not very interesting to him, so he willingly handed it over to others. He hardly noticed the lack of administrative machinery, a prerequisite for any scholarly museum. It was not easy to follow him on his flights of fancy and still build up the necessary support base. However, the fact that he in no way spared himself had an appeasing effect.Provincial museums at that time were of a mixed nature. A few had trained management, and the rest were run by interested locals. This was often excellently done, as in Esbjerg, where the master joiner Niels Thomsen and a staff of volunteers carried out excavations that were as good as professional investigations, and published them in well-written articles. Regrettably, there were also examples of the opposite. A museum curator in Jutland informed me that his predecessor had been an eager excavator but very rarely left any written documentation of his actions. The excavated items were left without labels in the museum store, often wrapped in newspapers. However, these gave a clue as to the time of unearthing, and with a bit of luck a look in the newspaper archive would then reveal where the excavation had taken place. Although somewhat exceptional, this is not the only such case.The Museum of Aarhus definitely belonged among the better ones in this respect. Founded in 1861, it was at first located at the then town hall, together with the local art collection. The rooms here soon became too cramped, and both collections were moved to a new building in the ”Mølleparken” park. There were skilful people here working as managers and assistants, such as Vilhelm Boye, who had received his archaeological training at the National Museum, and later the partners A. Reeh, a barrister, and G.V. Smith, a captain, who shared the honour of a number of skilfully performed excavations. Glob’s predecessor as curator was the librarian Ejler Haugsted, also a competent man of fine achievements. We did not, thus, take over a museum on its last legs. On the other hand, it did not meet the requirements of a modern scholarly museum. We were given the task of turning it into such a museum, as implied by the name change.The goal was to create a museum similar to the National Museum, but without the faults and shortcomings that that museum had developed over a period of time. In this respect our nightly conversations during our years in Copenhagen turned out to be useful, as our talk had focused on these imperfections and how to eradicate them.We now had the opportunity to put our theories into practice. We may not have succeeded in doing so, but two areas were essentially improved:The numerous independent numbering systems, which were familiar to us from the National Museum, were permeating archaeological excavation s not only in the field but also during later work at the museum. As far as possible this was boiled down to a single system, and a new type of report was born. (In this context, a ”report” is the paper following a field investigation, comprising drawings, photos etc. and describing the progress of the work and the observations made.) The instructions then followed by the National Museum staff regarding the conduct of excavations and report writing went back to a 19th-century protocol by the employee G.V. Blom. Although clear and rational – and a vast improvement at the time – this had become outdated. For instance, the excavation of a burial mound now involved not only the middle of the mound, containing the central grave and its surrounding artefacts, but the complete structure. A large number of details that no one had previously paid attention to thus had to be included in the report. It had become a comprehensive and time-consuming work to sum up the desultory notebook records in a clear and understandable description.The instructions resulting from the new approach determined a special records system that made it possible to transcribe the notebook almost directly into a report following the excavation. The transcription thus contained all the relevant information concerning the in vestigation, and included both relics and soil layers, the excavation method and practical matters, although in a random order. The report proper could then bereduced to a short account containing references to the numbers in the transcribed notebook, which gave more detailed information.As can be imagined, the work of reform was not a continuous process. On the contrary, it had to be done in our spare hours, which were few and far between with an employer like Glob. The assignments crowded in, and the large Jutland map that we had purchased was as studded with pins as a hedge hog’s spines. Each pin represented an inuninent survey, and many of these grew into small or large excavations. Glob himself had his lecture duties to perform, and although he by no means exaggerated his concern for the students, he rarely made it further than to the surveys. Bibby and I had to deal with the hard fieldwork. And the society, once it was established, did not make our lives any easier. Kuml demanded articles written at lightning speed. A perusal of my then diary has given me a vivid recollection of this hectic period, in which I had to make use of the evening and night hours, when the museum was quiet and I had a chance to collect my thoughts. Sometimes our faithful supporter, the Lord Mayor, popped in after an evening meeting. He was extremely interested in our problems, which were then solved according to our abilities over a cup of instant coffee.A large archaeological association already existed in Denmark. How ever, Glob found it necessary to establish another one which would be less oppressed by tradition. Det kongelige nordiske Oldsskriftselskab had been funded in 1825 and was still influenced by different peculiarities from back then. Membership was not open to everyone, as applications were subject to recommendation from two existing members and approval by a vote at one of the monthly lecture meetings. Most candidates were of course accepted, but unpopular persons were sometimes rejected. In addition, only men were admitted – women were banned – but after the war a proposal was brought forward to change this absurdity. It was rejected at first, so there was a considerable excitement at the January meeting in 1951, when the proposal was once again placed on the agenda. The poor lecturer (myself) did his best, although he was aware of the fact that just this once it was the present and not the past which was the focus of attention. The result of the voting was not very courteous as there were still many opponents, but the ladies were allowed in, even if they didn’t get the warmest welcome.In Glob’s society there were no such restrictions – everyone was welcome regardless of sex or age. If there was a model for the society, it was the younger and more progressive Norwegian Archaeological Society rather than the Danish one. The main purpose of both societies was to produce an annual publication, and from the start Glob’s Kuml had a closer resemblance to the Norwegian Viking than to the Danish Aarbøger for nordisk Oldkyndighed og Historie. The name of the publication caused careful consideration. For a long time I kept a slip of paper with different proposals, one of which was Kuml, which won after having been approved by the linguist Peter Skautrup.The name alone, however, was not enough, so now the task became to find so mething to fill Kuml with. To this end the finds came in handy, and as for those, Glob must have allied him self with the higher powers, since fortune smiled at him to a considerable extent. Just after entering upon his duties in Aarhus, an archaeological sensation landed at his feet. This happened in May 1950 when I was still living in the capital. A few of us had planned a trip to Aarhus, partly to look at the relics of th e past, and partly to visit our friend, the professor. He greeted us warmly and told us the exciting news that ten iron swords had been found during drainage work in the valley of lllerup Aadal north of the nearby town of Skanderborg. We took the news calmly as Glob rarely understated his affairs, but our scepticism was misplaced. When we visited the meadow the following day and carefully examined the dug-up soil, another sword appeared, as well as several spear and lance heads, and other iron artefacts. What the drainage trench diggers had found was nothing less than a place of sacrifice for war booty, like the four large finds from the 1800s. When I took up my post in Aarhus in September of that year I was granted responsibility for the lllerup excavation, which I worked on during the autumn and the following six summers. Some of my best memories are associated with this job – an interesting and happy time, with cheerful comradeship with a mixed bunch of helpers, who were mainly archaeology students. When we finished in 1956, it was not because the site had been fully investigated, but because the new owner of the bog plot had an aversion to archaeologists and their activities. Nineteen years later, in 1975, the work was resumed, this time under the leadership of Jørgen Ilkjær, and a large amount of weaponry was uncovered. The report from the find is presently being published.At short intervals, the year 1952 brought two finds of great importance: in Februar y the huge vessel from Braa near Horsens, and in April the Grauballe Man. The large Celtic bronze bowl with the bulls’ heads was found disassembled, buried in a hill and covered by a couple of large stones. Thanks to the finder, the farmer Søren Paaske, work was stopped early enough to leave areas untouched for the subsequent examination.The saga of the Grauballe Man, or the part of it that we know, began as a rumour on the 26th of April: a skeleton had been found in a bog near Silkeborg. On the following day, which happened to be a Sunday, Glob went off to have a look at the find. I had other business, but I arrived at the museum in the evening with an acquaintance. In my diary I wrote: ”When we came in we had a slight shock. On the floor was a peat block with a corpse – a proper, well-preserved bog body. Glob brought it. ”We’ll be in trouble now.” And so we were, and Glob was in high spirits. The find created a sensation, which was also thanks to the quick presentation that we mounted. I had purchased a tape recorder, which cost me a packet – not a small handy one like the ones you get nowadays, but a large monstrosity with a steel tape (it was, after all, early days for this device) – and assisted by several experts, we taped a number of short lectures for the benefit of the visitors. People flocked in; the queue meandered from the exhibition room, through the museum halls, and a long way down the street. It took a long wait to get there, but the visitors seemed to enjoy the experience. The bog man lay in his hastily – procured exhibition case, which people circled around while the talking machine repeatedly expressed its words of wisdom – unfortunately with quite a few interruptions as the tape broke and had to be assembled by hand. Luckily, the tape recorders now often used for exhibitions are more dependable than mine.When the waves had died down and the exhibition ended, the experts examined the bog man. He was x-rayed at several points, cut open, given a tooth inspection, even had his fingerprints taken. During the autopsy there was a small mishap, which we kept to ourselves. However, after almost fifty years I must be able to reveal it: Among the organs removed for investigation was the liver, which was supposedly suitable for a C-14 dating – which at the time was a new dating method, introduced to Denmark after the war. The liver was sent to the laboratory in Copenhagen, and from here we received a telephone call a few days later. What had been sent in for examination was not the liver, but the stomach. The unfortunate (and in all other respects highly competent) Aarhus doctor who had performed the dissection was cal1ed in again. During another visit to the bogman’s inner parts he brought out what he believed to be the real liver. None of us were capable of deciding th is question. It was sent to Copenhagen at great speed, and a while later the dating arrived: Roman Iron Age. This result was later revised as the dating method was improved. The Grauballe Man is now thought to have lived before the birth of Christ.The preservation of the Grauballe Man was to be conservation officer Kornbak’s masterpiece. There were no earlier cases available for reference, so he invented a new method, which was very successful. In the first volumes of Kuml, society members read about the exiting history of the bog body and of the glimpses of prehistoric sacrificial customs that this find gave. They also read about the Bahrain expeditions, which Glob initiated and which became the apple of his eye. Bibby played a central role in this, as it was he who – at an evening gathering at Glob’s and Harriet’s home in Risskov – described his stay on the Persian Gulf island and the numerous burial mounds there. Glob made a quick decision (one of his special abilities was to see possibilities that noone else did, and to carry them out successfully to everyone’s surprise) and in December 1952 he and Bibby left for the Gulf, unaware of the fact that they were thereby beginning a series of expeditions which would continue for decades. Again it was Glob’s special genius that was the decisive factor. He very quickly got on friendly terms with the rulers of the small sheikhdoms and interested them in their past. As everyone knows, oil is flowing plentifully in those parts. The rulers were thus financially powerful and some of this wealth was quickly diverted to the expeditions, which probably would not have survived for so long without this assistance. To those of us who took part in them from time to time, the Gulf expeditions were an unforgettable experience, not just because of the interesting work, but even more because of the contact with the local population, which gave us an insight into local manners and customs that helped to explain parts of our own country’s past which might otherwise be difficult to understand. For Glob and the rest of us did not just get close to the elite: in spite of language problems, our Arab workers became our good friends. Things livened up when we occasionally turned up in their palm huts.Still, co-operating with Glob was not always an easy task – the sparks sometimes flew. His talent of initiating things is of course undisputed, as are the lasting results. He was, however, most attractive when he was in luck. Attention normally focused on this magnificent person whose anecdotes were not taken too seriously, but if something went wrong or failed to work out, he could be grossly unreasonable and a little too willing to abdicate responsibility, even when it was in fact his. This might lead to violent arguments, but peace was always restored. In 1954, another museum curator was attached to the museum: Poul Kjærum, who was immediately given the important task of investigating the dolmen settlement near Tustrup on Northern Djursland. This gave important results, such as the discovery of a cult house, which was a new and hitherto unknown Stone Age feature.A task which had long been on our mind s was finally carried out in 1955: constructing a new display of the museum collections. The old exhibitio n type consisted of numerous artefacts lined up in cases, accompaied ony by a brief note of the place where it was found and the type – which was the standard then. This type of exhibition did not give much idea of life in prehistoric times.We wanted to allow the finds to speak for themselves via the way that they were arranged, and with the aid of models, photos and drawings. We couldn’t do without texts, but these could be short, as people would understand more by just looking at the exhibits. Glob was in the Gulf at the time, so Kjærum and I performed the task with little money but with competent practical help from conservator Kornbak. We shared the work, but in fairness I must add that my part, which included the new lllerup find, was more suitable for an untraditional display. In order to illustrate the confusion of the sacrificial site, the numerous bent swords and other weapons were scattered a.long the back wall of the exhibition hall, above a bog land scape painted by Emil Gregersen. A peat column with inlaid slides illustrated the gradual change from prehistoric lake to bog, while a free-standing exhibition case held a horse’s skeleton with a broken skull, accompanied by sacrificial offerings. A model of the Nydam boat with all its oars sticking out hung from the ceiling, as did the fine copy of the Gundestrup vessel, as the Braa vessel had not yet been preserved. The rich pictorial decoration of the vessel’s inner plates was exhibited in its own case underneath. This was an exhibition form that differed considerably from all other Danish exhibitions of the time, and it quickly set a fashion. We awaited Glob’s homecoming with anticipation – if it wasn’t his exhibition it was still made in his spirit. We hoped that he would be surprised – and he was.The museum was thus taking shape. Its few employees included Jytte Ræbild, who held a key position as a secretary, and a growing number of archaeology students who took part in the work in various ways during these first years. Later, the number of employees grew to include the aforementioned excavation pioneer Georg Kunwald, and Hellmuth Andersen and Hans Jørgen Madsen, whose research into the past of Aarhus, and later into Danevirke is known to many, and also the ethnographer Klaus Ferdinand. And now Moesgaard appeared on the horizon. It was of course Glob’s idea to move everything to a manor near Aarhus – he had been fantasising about this from his first Aarhus days, and no one had raised any objections. Now there was a chance of fulfilling the dream, although the actual realisation was still a difficult task.During all this, the Jutland Archaeological Society thrived and attracted more members than expected. Local branches were founded in several towns, summer trips were arranged and a ”Worsaae Medal” was occasionally donated to persons who had deserved it from an archaeological perspective. Kuml came out regularly with contributions from museum people and the like-minded. The publication had a form that appealed to an inner circle of people interested in archaeology. This was the intention, and this is how it should be. But in my opinion this was not quite enough. We also needed a publication that would cater to a wider public and that followed the same basic ideas as the new exhibition.I imagined a booklet, which – without over-popularsing – would address not only the professional and amateur archaeologist but also anyone else interested in the past. The result was Skalk, which (being a branch of the society) published its fir t issue in the spring of 1957. It was a somewhat daring venture, as the financial base was weak and I had no knowledge of how to run a magazine. However, both finances and experience grew with the number of subscribers – and faster than expected, too. Skalk must have met an unsatisfied need, and this we exploited to the best of our ability with various cheap advertisements. The original idea was to deal only with prehistoric and medieval archaeology, but the historians also wanted to contribute, and not just the digging kind. They were given permission, and so the topic of the magazine ended up being Denmark’s past from the time of its first inhabitant s until the times remembered by the oldest of us – with the odd sideways leap to other subjects. It would be impossible to claim that Skalk was at the top of Glob’s wish list, but he liked it and supported the idea in every way. The keeper of national antiquities, Johannes Brøndsted, did the same, and no doubt his unreserved approval of the magazine contributed to its quick growth. Not all authors found it easy to give up technical language and express themselves in everyday Danish, but the new style was quickly accepted. Ofcourse the obligations of the magazine work were also sometimes annoying. One example from the diary: ”S. had promised to write an article, but it was overdue. We agreed to a final deadline and when that was overdue I phoned again and was told that the author had gone to Switzerland. My hair turned grey overnight.” These things happened, but in this particular case there was a happy ending. Another academic promised me three pages about an excavation, but delivered ten. As it happened, I only shortened his production by a third.The 1960s brought great changes. After careful consideration, Glob left us to become the keeper of national antiquities. One important reason for his hesitation was of course Moesgaard, which he missed out on – the transfer was almost settled. This was a great loss to the Aarhus museum and perhaps to Glob, too, as life granted him much greater opportunities for development.” I am not the type to regret things,” he later stated, and hopefully this was true. And I had to choose between the museum and Skalk – the work with the magazine had become too timeconsuming for the two jobs to be combined. Skalk won, and I can truthfully say that I have never looked back. The magazine grew quickly, and happy years followed. My resignation from the museum also meant that Skalk was disengaged from the Jutland Archaeological Society, but a close connection remained with both the museum and the society.What has been described here all happened when the museum world was at the parting of the ways. It was a time of innovation, and it is my opinion that we at the Prehistoric Museum contributed to that change in various ways.The new Museum Act of 1958 gave impetus to the study of the past. The number of archaeology students in creased tremendously, and new techniques brought new possibilities that the discussion club of the 1940s had not even dreamt of, but which have helped to make some of the visions from back then come true. Public in terest in archaeology and history is still avid, although to my regret, the ahistorical 1960s and 1970s did put a damper on it.Glob is greatly missed; not many of his kind are born nowadays. He had, so to say, great virtues and great fault s, but could we have done without either? It is due to him that we have the Jutland Archaeological Society, which has no w existed for half a century. Congr tulat ion s to the Society, from your offspring Skalk.Harald AndersenSkalk MagazineTranslated by Annette Lerche Trolle

AbstractCoelastrum pascheri sp. n. is described. It is similar to Coelastrum morus W. et G. S. West sensu Skuja (1930) in the morphology of the free processes, but differs from it in cell size (it is significantly smaller, 13–16 µm) and in the number of cells in a cenobium (usually 4). The new alga was found in the littoral of some lakes: Černé jezero (Černé Lake), Grosser Arbersee and Kleiner Arbersee in the Bohemian Forest (Šumava, Böhmerwald) and also in Sphagnum within a small puddle in the Upolínová meadow in the Slavkovský les Mts.

There are still many uncertainties about the climatic forcing that drove the glacier fluctuations of the Patagonian Ice Sheet (PIS, 38–55°S) during the last glacial period. A key source of uncertainty is the asynchrony of ice lobe fluctuations between the northern, central, and southern PIS. To fully understand the regional trends requires careful mapping and extensive geochronological studies. This paper presents geomorphological and geochronological reconstructions of the glacial and deglacial landforms formed during the last glacial period at the Seno Skyring lobe, southernmost Patagonia (52°S, 71°W). We present a detailed geomorphological map, where we identify two moraine systems. The outer and older is named Laguna Blanca (LB) and the inner Río Verde (RV). The LB moraines were built subaerially, whereas parts of the RV were deposited subaqueously under the palaeo lake Laguna Blanca, which developed during deglaciation. We conducted surface exposure 10Be dating methods on boulder samples collected from LB and RV glacial margins. The moraine LB III and LB IV formed at 26.3 ± 2.3 ka (n = 5) and 24.3 ± 0.9 ka (n = 3), respectively. For the inner RV moraine, we obtained an age of 18.7 ± 1.5 ka (n = 6). For the palaeo Laguna Blanca evolution, we performed 10Be exposure ages on shoreline berms and optically stimulated luminesce dating to constrain the lake levels, and 10Be depth profile dating on an outwash deposit formed by a partial lake drainage event, which occurred at 22 ± 3 ka. For the RV moraine deglaciation, we performed radiocarbon dating of basal sediments in a peat bog, which indicates that the glacier retreated from the terminal RV moraine by at least c. 16.4 cal kyr BP. Our moraine geochronology shows an asynchrony in the maximum extents and a different pattern of ice advances between neighbouring lobes in southern Patagonia. We speculate that this may be due, at least in part, to the interaction between topography and the precipitation carried by the southern westerly wind belt. However, we found broad synchrony of glacial readvances contemporaneous with the RV moraine.

The environmental variability of Northern Chilean Patagonia during the last millennia is evaluated using a multi-proxy analysis of sediment cores from Lake Esponja (45°S 72°W) to decipher if the sediment deposition is controlled by volcanic eruptions, landslides induced by earthquake or heavy rainfall. The lake is located in a glacio-tectonic valley in Patagonia. The organic-rich clayey silt sediment with low biogenic silica content was analysed for grain size, magnetic susceptibility, organic matter, biogenic silica content and diatom assemblages, mineralogy (X-ray diffraction), organic (IRMS C and N analyses) and inorganic (XRF core-scanner) geochemistry and glass shard major composition (Microprobe, SEM). The combination of 210Pb, 137Cs, 14C and tephrochronology indicates an averaged accumulation rate of 0.4 mm/year, leading to a record of ~3.5 kyr within 154 cm. The sedimentary geochemistry records changes in volcanic supplies, diatom productivity and detrital inputs. The sediments were interrupted by millimetric to centimetric layers corresponding to tephra deposition related to explosive eruptions of nearby volcanoes Macá, Melimoyu and Hudson. Concerning the diatoms, the dominant planktonic species (80–150 cm) are replaced by benthic species in a transition interval (55–80 cm) and then by Surirella spp. in the upper core. This last genus indicates a closure of the basin ~2 ka ago, probably related to an uplift linked to a rejuvenation of the Mañihuales fault. This local change could reflect regional tectonic instability. Indeed, a partial earthquake rupture occurred around ~AD100 along the southern part of the Valdivia segment, recorded as a mass transport deposit in Aysén fjord sedimentation. The fine detrital input varies over time with more variable Si/Al values in the lower part of the LEs14 core than in the upper 80 cm. The higher values may reflect wetter conditions, leading to an higher lake level and more turbid conditions in agreement with changes in diatom assemblages.

AbstractHarmful blooms of the filamentous cyanobacteria Planktothrix rubescens have become common in many lakes as they have recovered from eutrophication over the last decades. These cyanobacteria, capable of regulating their vertical position, often flourish at the thermocline to form a deep chlorophyll maximum. In Lake Zurich (Switzerland), they accumulate during stratified season (May–October) as a persistent metalimnetic thin layer (~2 m wide). This study investigated the role of turbulent mixing in springtime layer formation, its persistence over the summer, and its breakdown in autumn. We characterised seasonal variation of turbulence in Lake Zurich with four surveys conducted in April, July and October of 2018 and September of 2019. Surveys included microstructure profiles and high-resolution mooring measurements. In July and October, the thin layer occurred within a strong thermocline ($$N \gtrsim 0.05$$ N ≳ 0.05 s$$^{-1}$$ - 1 ) and withstood significant turbulence, observed as turbulent kinetic energy dissipation rates ($$\varepsilon \approx 10^{-8}$$ ε ≈ 10 - 8 W kg$$^{-1}$$ - 1 ). Vertical turbulent overturns –monitored by the Thorpe scale– went mostly undetected and on average fell below those estimated by the Ozmidov scale ($$L_O \approx 1$$ L O ≈ 1 cm). Consistently, vertical diffusivity was close to molecular values, indicating negligible turbulent fluxes. This reduced metalimnetic mixing explains the persistence of the thin layer, which disappears with the deepening of the surface mixed layer in autumn. Bi-weekly temperature profiles in 2018 and a nighttime microstructure sampling in September 2019 showed that nighttime convection serves as the main mechanism driving the breakdown of the cyanobacterial layer in autumn. These results highlight the importance of light winds and convective mixing in the seasonal cycling of P. rubescens communities within a strongly stratified medium-sized lake.

The IRAW@Bagan project is aimed at developing an integrated socio-ecological history for residential patterning, agricultural practices, and water management at the classical Burmese (Bama) capital of Bagan, Myanmar (11th to 14th centuries CE). As part of this long-term research program investigations have been initiated in the Tuyin-Thetso uplands, located 11 km southeast of Bagan’s walled and moated epicenter. This mountainous area figures prominently in the chronicles of early Bagan, given that it was one of five places around the city that a royal white elephant carrying a Buddhist tooth-relic kneeled down, prompting King Anawrahta (1044-1077 CE) to build a pagoda (i.e., temple) there. Numerous 13th century religious monuments were subsequently built on the Tuyin Range. Recent explorations in these uplands have drawn attention to an additional feature of historical significance, a rock-cut tank located along the eastern edge of the Thetso-Taung ridge. Referred to by local villagers as Nat Yekan (Spirit Lake), this reservoir appears to have been integral not only to the initial collection and subsequent redistribution of water across the Bagan plain via a series of interconnected canals and reservoirs, but also, through its associated iconographic imagery, it may have been intended to symbolically purify this water, enhancing its fertility prior to its flowing into the city’s peri-urban zone. Hydrological modelling, excavations, and both iconographic and epigraphic analysis are used to build a multilayered understanding of Nat Yekan’s economic, political, religious, and ideological significance during Bagan’s classical era.IRAW Bagan စီမံကိန္◌းသည္ ၁၁ရာစ0မွ ၁၄ရာစ0အ4တင္◌း စ789ငန: ္◌းကားခ့ေဲ ◌သာ◌ျမ@Aာတိ0႔၏ ပ0ဂံႏ◌ိ◌0ငGံေ◌တာ◌္4တင္ ဘက္ေ◌ပါင္◌းစံ◌0ေ◌ပါင္◌းစည္◌းထားေ◌သာ လLမႈေ◌ဂဟ သမင0ိ ္◌းေ◌ၾကာင္◌း◌ျဖစ္ေ◌သာ လLေ◌နထ0ိငႈA ပံ◌စ0 ံ၊ စ0ိကး8် ေိ ◌ဳ ရး ေဓလစ့ ႐0ိကA်ား◌ႏ◌ွင္◌ ့ ေ◌ရအရင◌္ းအျမစသV ံ◌0◌းခ်မႈမ်ားအား ◌ျပ@Wည္ေ◌ဖာ◌္ထ0တXန ္အဓိကရY7Xယ8ါသည္။ အဆိ0ပါ ေ◌ရရွည္ သ0ေ◌တသန စီမံကိန္◌း ေ◌လ့လာေ◌ရးအားဗဟ0ိအခ်ကV ခ်ာ◌ျဖစ္ေ◌သာ ပ0ဂံၿမိေဳ႕ဟာင္◌း၏ ေအရွေ႕တာငက` ္ ၁၁ကီလ0ိမီတာေ◌ဝး4ကာေ◌သာ တ0ရင္ ◌ႏ◌ွင◌္ ့ သကbိcးေ◌တာင္ ဧရိယာမွ စတငWcပ္ေ◌ဆာငပeဲ ့ ါသည္။ေ၎တာငgန္◌းဧရိယာသည္ ပ0ဂံရာဇဝ9ငgင္ ေအနာ◌္ရထာမင္◌းႀကီး (၁၀၄၄-၁၀၇၇ AD)အဓိဠာန္◌ျပဳ၍ လႊတW ိcက္ေ◌သာ ဗ0ဒၶျမYတbယ္ေ◌တာ◌ ္ တင္ေ◌ဆာငqည္◌ ့ဆင္◌ျဖဴေ◌တာ◌္ကိန္◌းဝပXာ ငါးေ◌နရာ4တင္ တစecအပါအဝင္◌ျဖစqည္◌ ့ထငာX းs ေအရးပါသည◌္ ့ ေ◌နရာလဲ ◌ျဖစ8ါသည္။ မ်ားြစာေ◌သာ ၁၃ရာစ0 ပ0ဂံေ◌ခတ္သာသနကိ ေအဆာကVအံ◌0 မ်ားလည္◌း တ0ရင္ေ◌တာင္ေ◌ၾကာ တစ္ေ◌လ်ာက္တည္ေ◌ဆာက:ား ၾကသည္။ အဆိ0ပါေ◌တာငgန္◌း4တင္ လေကgလာ စLးစမ္◌းရွာေ◌ြဖမႈသ7Wည္◌း သမ0ိင္◌းတ@vိcးအရ သာသနကိ ေအဆာကVအံ◌0မ်ား ကဲ့သ0ိ႔ေအရးပါေ◌သာသကbိcးေ◌တာင္၏ ေအရွ႕ဖကV ြစန္◌း4တင္ တ7Xိေs◌သာ ေ◌က်က္ေ◌ရက@ာV းအထLး◌ျပဳေ◌လ့လာ◌ျခင္◌း◌ျဖစqည္။ ေ◌ဒသခံ wxာသLwxာသားမ်ားက ေ၎က်ာက္ေ◌ရက@Vားနတ္ေ◌ရက@yc ေ◌ခၚဆိ0မႈအရ ေ၎ေရလွာင{@|ကီးသည္ ကနဦး ေ◌ရစ0ေ◌ဆာင္◌းသိ0ေ◌လွာင~ပီးေ◌နာက္ ပ0ဂံ4လင္◌ျပင္ တစ္ေ◌လ်ာကXိ s ေ◌ေရလွာင@{ ာ်A းတLးေ◌◌ျမာင္◌းမ်ားႏ◌ွင◌္ ့ ဆကqယ္၍ ေ◌xျပ@Wည ္ ◌ျဖန္ေ႔ေဝပးယံ◌0သာ မကဘ ဲ4ထင္◌းထ0ထားေ◌သာ ႐0ပWံ◌0◌း႐0ပ္◌ႂ4ကမ်ားသ7Wည္◌း ကန္ေ◌ရအား ဒ0မဂၤလသန္႔ရွင္◌းစငက‚ ယAႈေသဘာေ◌ဆာင္၍ ◌ျဖန္ေ႔ဝျခင္◌း◌ျဖင္◌ ့ ပ0ဂံၿမိ႕ဳအနးီ တဝ0ိက္၎ကန္ေ◌ရရာရွိရာ ေ◌နရာေ◌ဒသမ်ား သာယာစ78င ္ ြဖံ႔ၿဖိးေဳ ◌အာင ္ ◌ျပဳလ0ပမbီ ံထားသ7ycမွတƒ „ရပါသည္။ ဂႏ◌ဝၲ ငc8 ဂံ ေ◌ခတV 4တင္◌း ထငာX းs ခ့ေဲ ◌သာ နတ္ေ◌ရကန္◌ႏ◌ွင္◌ ့ပတqတ†က္◌ႏြ◌ယ္ေ◌ေနသာ စီးြပားေ◌ရး၊ ◌ႏ◌ိ◌0ငGံေ◌ရး၊ ဘာသာေ◌ရးစသည္◌ေ့ သဘာတရားေ◌ရးရာ အဆင◌္ ဆ့ င္◌အ့ ား နားလည္ေ◌စရန္ေ◌ရအရင္◌းအျမစVသံ◌0◌းခ်သိပၸံပညာ၊ ေ◌ရွးေ◌ဟာင္◌းသ0ေ◌တသန တLးေ◌ဖာ◌္မႈ◌ႏ◌ွင္◌အ့ တL ႐0ပWံ◌◌0 း႐0ပ◌္ ေႂ 4ကလ့လာမႈ ◌ႏ◌ွင္◌ ့ ေ◌က်ာကbာ စိစစ္ေ◌4တ႕ရွိခ်ကA်ားအားအသံ◌0◌း◌ျပဳ ေ◌လ့လာ တင္◌ျပ4သားပါမည္။