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Rishi, Bikramjit, and Archit Kacker. "Kingfisher: Ultra Max re-positioning hurdle." Emerald Emerging Markets Case Studies 11, no. 2 (May 21, 2021): 1–19. http://dx.doi.org/10.1108/eemcs-09-2020-0322.
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Learning outcomes To appraise the product positioning in an emerging market. To recognize and discuss a positioning plan for a new product. To apply, describe the Kapferer brand identity prism along with different competition levels for Kingfisher Ultra Max. To deliberate the marketing mix for improving the sales of Kingfisher Ultra Max. Case overview/synopsis United Breweries Limited (UBL) was part of UB Group, which was a business conglomerate. United Breweries Holdings Limited or UB Group was headquartered at UB City, Bangalore, India. It dealt in many businesses, out of which UBL was one of them. Kingfisher Ultra Max was Kingfisher’s newest addition to the super-premium strong beer segment. It was a larger-based beer with 8% alcohol by volume content and was stronger in terms of alcohol content than Kingfisher Ultra, which was also from the super-premium segment. This brands positioning was such that it targeted the premium segment. The top management was considering a change in positioning for their Ultra Max brand. A research report submitted by a premier business school also recommended a change in positioning. The officials in the meeting are contemplating the two options for the shift in positioning; one is to make the change of positioning across India and the other is to make the change specific to some states. Complexity academic level The case is targeted at students of post-graduation and under-graduation programs in business administration, specializing in marketing management, brand management or marketing strategy. Also, the case study can be included as part of courses related to strategic management and competitive analysis. Supplementary materials Teaching Notes are available for educators only. Please contact your library to gain login details or email support@emeraldinsight.com to request teaching notes. Subject code CSS 8: Marketing. Supplementary materials Teaching Notes are available for educators only.
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Jazdzewska, Katarzyna. "‘Like a Married Woman’." Mnemosyne 68, no. 3 (April 24, 2015): 424–36. http://dx.doi.org/10.1163/1568525x-12301586.
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This contribution examines Plutarch’s depiction of the kingfisher in De sollertia animalium 982e-983e and argues that it presents the bird as embodiment of three uxorial virtues: love for husband, love for offspring, and care for household. While Plutarch clearly draws from the abundant store of Greek kingfisher-lore, his account explicates the moralizing potential of the kingfisher-exemplum in a manner unparalleled in extant earlier tradition. In his composition of the passage, Plutarch might have been inspired by the pseudo-Platonic dialogue Halcyon with which the kingfisher-passage in De sollertia animalium shares numerous remarkable resemblances.
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Agung Santosa, Rizki, Sugeng P. Harianto, and Nuning Nurcahyani. "Perbandingan Populasi Burung Cekakak (Halcyonidae) Di Lahan Basah Desa Sungai Luar Dan Lahan Basah Desa Kibang Pacing Kecamatan Menggala Timur Kabupaten Tulang Bawang." Jurnal Sylva Lestari 4, no. 2 (May 23, 2016): 79. http://dx.doi.org/10.23960/jsl2479-88.
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Wetland as one of natural habitat for wild animals in Indonesia have been changed a lot. Wetland at Sungai Luar and Kibang Pacing are the nature habitat for settler birds diversity and place to stop over for migratory birds. The purpose of this research is to know, the population of kingfisher (Halcyonidae) and population ratio at two research location. Method used in this research arethe IPA method (Indicates Pountuele d’Abudance – Abudance Index On the Point) and the distributing model. This observation was done by staying at the point count and making a note for every founded birds. For each community has three point count witheach 18 days observation. To calculate population ratio of the kingfisher (Halcyonidae) birds at two research locations ware used the formula of Yuniar (2007), that is P= X ± Se.t. The result showed that from the two locations have been founded just one species from Halcyonidae that is the white-throated kingfisher (Halcyon smyrnensis). The population have been founded in Sungai Luaras many 18 birds. The population in Kibang Pacingwetland as many 12 birds. Population ratio of White-throated kingfisher (Halcyon smyrnensi) in Sungai Luar and Kibang Pacing wetland are1.5:1 bird (60% :40%). Distribution model of the bird at two locations research is systematic. Keywords: halcyonidae, population, wetland, sungai luar, Kibang Pacing
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VAN DER VLIET, ROLAND E., ALICE CIBOIS, ANITA GAMAUF, and JUSTIN J. F. J. JANSEN. "Origins, identification and type status of two early specimens of Society Kingfisher Todiramphus veneratus (J. F. Gmelin, 1788) (Aves: Coraciiformes, Alcedinidae)." Zootaxa 4691, no. 3 (October 31, 2019): 250–60. http://dx.doi.org/10.11646/zootaxa.4691.3.5.
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We re-examined the putative type specimen of Society Kingfisher Todiramphus veneratus (J. F. Gmelin, 1788) in the Naturhistorisches Museum Wien (NMW 50.633) and conclude based on plumage that it represents the taxon from Moorea, T. veneratus youngi Sharpe, 1892, rather than nominate T. veneratus veneratus from Tahiti. X-rays reveal that it was prepared using techniques common in the late 18th century, and that its preparation style matches that of other specimens collected during Cook’s three voyages. NMW 50.633 has been assumed to be the one, or one of a number of, specimen(s) used by Latham to describe and illustrate his ‘Venerated Kingfisher’ (present-day Society Kingfisher), which was the basis of the later valid introduction of the name Alcedo venerata by J. F. Gmelin. However, whereas the description and an unpublished illustration in Latham’s archives agree closely with veneratus from Tahiti, NMW 50.633 appears to represent Moorea youngi. While this finding does not compromise the definition of Society Kingfisher veneratus, it leaves it without a safely identified type specimen. We also examined a Moorea specimen in the National Museums Liverpool (LIVCM D2366) that is almost as old as NMW 50.633, but which X-rays suggest had a different origin than NMW 50.633.
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Spence, Samantha, and Helge Kreutz. "The Kingfisher Field, Block 16/8a, UK North Sea." Geological Society, London, Memoirs 20, no. 1 (2003): 305–14. http://dx.doi.org/10.1144/gsl.mem.2003.020.01.26.
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AbstractThe Kingfisher Field is located in the South Viking Graben, Block 16/8a, with a minor extension into Block 16/8c. Block 16/8 was initially awarded in June 1970 to Shell and Esso, with the Kingfisher discovery well 16/8-1 spudded in 1972. The well tested high H2S oil at marginal rates from Upper Jurassic Brae Formation sandstones. Subsequent appraisal well 16/8a-4 (1984) tested gas/condensate from better quality Brae Formation sandstone reservoirs. This well also discovered the deeper Middle Jurassic Heather Formation sandstone gas/condensate accumulation at near-HPHT conditions. The Brae and Heather Formation sandstones contain stacked hydrocarbon accumulations in separate combinations of stratigraphic and structural traps. Production by natural aquifer drive commenced from a sub-sea satellite to Marathon's Brae B platform in 1997, initially from the Brae reservoirs. To date, three production wells have been completed and a fourth well is planned to be on stream in 2000. The Brae Formation sandstones at Kingfisher are interpreted as distal deposits of the Brae/Miller fan-apron system and range in quality from excellent to very poor across the field. The Heather Formation reservoir consists of medium quality sands deposited within a submarine incised valley. The most recent volumetric estimate (1998) for the total field predicts an ultimate recovery of 41.2 MMBBL of pipeline liquids and 280 BCF of dry export gas. Regional reservoir architecture and connectivity as well as hydrocarbon composition are key to understanding the production performance of the critical gas/condensate below dewpoint. Advances in sub-sea and horizontal drilling technology have enabled field development.
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Younes, A. M., G. O. Morrell, and A. B. Thompson. "USE OF COMPUTER GRAPHICS: THE WEST KINGFISH POST DEVELOPMENT RESERVOIR SIMULATION STUDY." APPEA Journal 26, no. 1 (1986): 447. http://dx.doi.org/10.1071/aj85038.
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The West Kingfish Field in the Gippsland Basin, offshore Victoria, has been developed from the West King-fish platform by Esso Australia Ltd (operator) and BHP Petroleum.The structure is an essentially separate, largely stratigraphic accumulation that forms the western flank of the Kingfish feature. A total of 19 development wells were drilled from the West Kingfish platform between October 1982 and May 1984. Information provided by these wells was used in a West Kingfish post-development geologic study and a reservoir simulation study.As a result of these studies the estimated recoverable oil volume has been increased 55 per cent to 27.0 stock tank gigalitres (170 million stock tank barrels). The studies also formed the technical basis for obtaining new oil classification of the P-1.1 reservoir which is the only sand body that has been found in the Gurnard Formation in the Kingfish area.The simulation study was accomplished with an extremely high level of efficiency due to the extensive and effective use of computer graphics technology in model construction, history matching and predictions.Computer graphics technology has also been used very effectively in presenting the simulation study results in an understandable way to audiences with various backgrounds. A portable microcomputer has been used to store hundreds of graphic displays which are projected with a large screen video projector.Presentations using this new display technology have been well received and have been very successful in conveying the results of a complex reservoir simulation study and in identifying future field development opportunities to audiences with various backgrounds.
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Clarke, TM, SK Whitmarsh, RG Dwyer, V. Udyawer, H. Pederson, and C. Huveneers. "Effects of shark tourism on the daily residency and movements of a non-focal pelagic teleost." Marine Ecology Progress Series 687 (April 7, 2022): 133–46. http://dx.doi.org/10.3354/meps13998.
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Marine wildlife tourism is increasing in popularity, with operations targeting a wide range of taxa globally. While previous studies have mostly focused on assessing the effects of provisioning from tourism on focal species, non-focal species that unintentionally feed on supplemental food sources have largely been overlooked. This study improves our understanding of the effects of shark-cage-diving tourism on the movements and behaviours of a non-focal pelagic fish. We used acoustic tracking to determine the effects of shark-cage-diving tourism on the residency and space use of 17 yellowtail kingfish Seriola lalandi at the Neptune Islands, South Australia. We revealed that while cage-diving did not affect the overall or weekly residency and space use of kingfish, daily time spent at the islands and location of kingfish was influenced by the presence of operators. Acoustic attractant did not affect kingfish behaviours, but operators using food-based attractants increased the average time spent at the Neptune Islands by ~27% (from 230.6 ± 6.8 to 293.8 ± 5.5 min). Kingfish were also observed closer to operators using food-based attractants (217 ± 4.82 m from vessel) compared to an acoustic attractant (412 ± 29.5 m from vessel). Our findings identify changes in the daily behaviour of kingfish at the Neptune Islands as a result of food-based attractants from shark-cage-diving, which demonstrates that non-focal large pelagic species can be affected by shark-diving tourism. These effects may lead to long-term effects on the physiological condition and energetic responses of these individuals.
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Abas, Mohd Firdaus Bin, Balbir Singh, Kamarul Arifin Ahmad, Eddie Yin Kwee Ng, Tabrej Khan, and Tamer A. Sebaey. "Dwarf Kingfisher-Inspired Bionic Flapping Wing and Its Aerodynamic Performance at Lowest Flight Speed." Biomimetics 7, no. 3 (August 29, 2022): 123. http://dx.doi.org/10.3390/biomimetics7030123.
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This paper aims to understand the aerodynamic performance of a bio-inspired flapping-wing model using the dwarf Kingfisher wing as the bionic reference. The paper demonstrates the numerical investigation of the Kingfisher-inspired flapping-wing followed by experimental validation to comprehend the results fully and examine the aerodynamic characteristics at a flight velocity of 4.4 m/s, with wingbeat frequencies of 11 Hz, 16 Hz, and 21 Hz, at various angles of rotation ranging from 0° to 20° for each stroke cycle. The motivation to study the performance at low speed is based on lift generation as a challenge at low speed as per quasi-steady theory. The temporal evolution of the mean force coefficients has been plotted for various angles of rotation. The results show amplification of the maximum value for the cycle average lift and drag coefficient as the rotation angle increases. The history of vertical force and the flow patterns around the wing is captured in a full cycle with asymmetric lift development in a single stroke cycle. It is observed from the results that the downstroke generates more lift force in magnitude compared to the upstroke. In addition to the rotation angle, lift asymmetry is also affected by wing–wake interaction. Experimental results reveal that there is a stable leading-edge vortex developed in the downstroke, which sheds during the upstroke. An optimum lift and thrust flapping flight can be achieved, with a lift coefficient of 3.45 at 12°. The experimental and parametric study results also reveal the importance of passive rotation in wings for aerodynamic performance and wing flexibility as an important factor for lift generation.
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Makhov, Alexander. "STOICISM AND ITS METAPHORS IN EARLY MODERN EMBLEM BOOKS." Odysseus. Man in History 29, no. 1 (September 20, 2021): 145–76. http://dx.doi.org/10.32608/1607-6184-2021-29-1-145-176.
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The moral and social doctrine of Stoicism, well known among Early Modern humanists, was popularized in the emblem books of the time. The tool of this popularization was the visual metaphor capable of conveying abstract ideas through concrete images. The main stoic notions (such as virtue, apatheia as a complete freedom from passions, constancy, patience, etc.) have found extremely diverse metaphorical equivalents in the visual language of emblems, where inanimate objects (e.g. rock, flint, anvil, tongs, cube, scales) as well as living creatures (kingfisher, turtledove, bear) could equally function as metaphors. Emblematics, being a kind of ars inveniendi, acted as a mechanism for inventing new metaphors to express old meanings. However, some traditional metaphors dating back to antiquity (for example, Plato’s comparison of the human soul to a chariot pulled by two horses – “reason” and “emotion”) were also rethought in the spirit of the Stoic doctrine.
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SKORACKI, MACIEJ, MARTIN HROMADA, and BOZENA SIKORA. "Quill mites of the family Syringophilidae (Acariformes: Prostigmata) parasitizing coraciiform birds (Aves: Coraciiformes)." Zootaxa 4802, no. 1 (June 22, 2020): 169–81. http://dx.doi.org/10.11646/zootaxa.4802.1.11.
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Three new species of the family Syringophilidae associated with birds of the pantropically distributed order Coraciiformes are described: two new species of the subfamily Picobiinae—Rafapicobia momoti sp. nov. ex the Rufous-capped Motmot Baryphthengus ruficapillus (Vieillot) (Momotidae) from Brazil, Rafapicobia brachypteraci sp. nov. ex the Rufous-headed Ground-Roller Atelornis crossleyi Sharpe (Brachypteraciidae) from Madagascar, and one new species of the subfamily Syringophilinae—Peristerophila coraciidus sp. nov. ex the Dollarbird Eurystomus orientalis (Linnaeus) (Coraciidae) from Papua New Guinea. Additionally, the Collared Kingfisher, Todiramphus chloris (Boddaert) (Alcedinidae), is recorded as a new host for Peristerophila meropis Skoracki et al., 2017. In all these host families, syringophilids are recorded for the first time. Moreover, our findings represent the first records of picobiines from coraciiform birds. Currently, the fauna of quill mites associated with coraciiforms includes six species grouped in the three genera: Rafapicobia Skoracki, 2011, Peristerophila Kethley, 1970, and Syringophilopsis Kethley, 1970. All these genera seem to be typically telluravian and have been also recorded from passeriform birds.
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Frommel, Andrea Y., Colin J. Brauner, Bridie J. M. Allan, Simon Nicol, Darren M. Parsons, Steve M. J. Pether, Alvin N. Setiawan, Neville Smith, and Philip L. Munday. "Organ health and development in larval kingfish are unaffected by ocean acidification and warming." PeerJ 7 (December 12, 2019): e8266. http://dx.doi.org/10.7717/peerj.8266.
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Anthropogenic CO2 emissions are causing global ocean warming and ocean acidification. The early life stages of some marine fish are vulnerable to elevated ocean temperatures and CO2 concentrations, with lowered survival and growth rates most frequently documented. Underlying these effects, damage to different organs has been found as a response to elevated CO2 in larvae of several species of marine fish, yet the combined effects of acidification and warming on organ health are unknown. Yellowtail kingfish, Seriola lalandi, a circumglobal subtropical pelagic fish of high commercial and recreational value, were reared from fertilization under control (21 °C) and elevated (25 °C) temperature conditions fully crossed with control (500 µatm) and elevated (1,000 µatm) pCO2 conditions. Larvae were sampled at 11 days and 21 days post hatch for histological analysis of the eye, gills, gut, liver, pancreas, kidney and liver. Previous work found elevated temperature, but not elevated CO2, significantly reduced larval kingfish survival while increasing growth and developmental rate. The current histological analysis aimed to determine whether there were additional sublethal effects on organ condition and development and whether underlying organ damage could be responsible for the documented effects of temperature on survivorship. While damage to different organs was found in a number of larvae, these effects were not related to temperature and/or CO2 treatment. We conclude that kingfish larvae are generally vulnerable during organogenesis of the digestive system in their early development, but that this will not be exacerbated by near-future ocean warming and acidification.
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Siepe, A. "Regeneration of Floodplain Biotopes on the Upper Rhine – The “Polder Altenheim” Case." Water Science and Technology 29, no. 3 (February 1, 1994): 281–88. http://dx.doi.org/10.2166/wst.1994.0121.
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The floodplain of the Upper Rhine and its biocoenoses have, through different river-regulatory activities over the last 175 years, undergone large scale degradation. At the same time flood protection for the downstream inhabitants has been greatly reduced. For reasons of flood protection, the “Polder Altenheim” in Baden-Württemberg, Germany southwest of Strasbourg, France, with so called retention flooding, was put into operation in 1987. The original floodplain had been diked for the previous 17 years, during which no flooding occurred. Since 1989 “ecological flooding” also is carried out. This has assisted in the regeneration of floodplain biotopes and promoted the floodplain biotic communities and the readaption of the bioceonosis to a regular flooding regime. The creation of new floodplain biotopes of early succession stages, particularly through geomorphodynamic processes, has followed the more than ten flood ocassions and typical biotic communities have colonised these sites. This will be presented together with selected examples of terrestrial and limnical species and communities. The following species and communities will be discussed: kingfisher Alcedo atthis, carabid communities (Coleoptera), the red alga Hildenbrandia rivularis (Rhodophyceae), the freshwater snail Theodoxus fluviatilis (Neritacea) and the freshwater bug Aphelocheirus aestivalis (Hydrocorisae).
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Munday, Philip L., Sue-Ann Watson, Darren M. Parsons, Alicia King, Neill G. Barr, Ian M. Mcleod, Bridie J. M. Allan, and Steve M. J. Pether. "Effects of elevated CO2 on early life history development of the yellowtail kingfish, Seriola lalandi, a large pelagic fish." ICES Journal of Marine Science 73, no. 3 (November 20, 2015): 641–49. http://dx.doi.org/10.1093/icesjms/fsv210.
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Abstract An increasing number of studies have examined the effects of elevated carbon dioxide (CO2) and ocean acidification on marine fish, yet little is known about the effects on large pelagic fish. We tested the effects of elevated CO2 on the early life history development and behaviour of yellowtail kingfish, Seriola lalandi. Eggs and larvae were reared in current day control (450 µatm) and two elevated CO2 treatments for a total of 6 d, from 12 h post-fertilization until 3 d post-hatching (dph). Elevated CO2 treatments matched projections for the open ocean by the year 2100 under RCP 8.5 (880 µatm CO2) and a higher level (1700 µatm CO2) relevant to upwelling zones where pelagic fish often spawn. There was no effect of elevated CO2 on survival to hatching or 3 dph. Oil globule diameter decreased with an increasing CO2 level, indicating potential effects of elevated CO2 on energy utilization of newly hatched larvae, but other morphometric traits did not differ among treatments. Contrary to expectations, there were no effects of elevated CO2 on larval behaviour. Activity level, startle response, and phototaxis did not differ among treatments. Our results contrast with findings for reef fish, where a wide range of sensory and behavioural effects have been reported. We hypothesize that the absence of behavioural effects in 3 dph yellowtail kingfish is due to the early developmental state of newly hatched pelagic fish. Behavioural effects of high CO2 may not occur until larvae commence branchial acid–base regulation when the gills develop; however, further studies are required to test this hypothesis. Our results suggest that the early stages of kingfish development are tolerant to rising CO2 levels in the ocean.
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Khot, Mayura. "A checklist of avifauna from Malgaon-Bagayat and Malvan towns of Sindhudurg District, Maharashtra, India." Journal of Threatened Taxa 8, no. 6 (June 26, 2016): 8909. http://dx.doi.org/10.11609/jott.1706.8.6.8909-8918.
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The present work was carried out in Malgaon-Bagayat (16009’04.35”N & 73033’04.7”E) and Malvan Taluka (16005’00”N & 73030’00”E) of Sindhudurg District. The paper represents the first document on birds as there are no published records from Sindhudurg District. The study was conducted for a period of two years to explore the avifauna as no records have been published yet from this area. A total of 101 species of birds belonging to 17 orders and 45 families and were recorded in the study areas inhabiting different types of habitats. The Order Passeriformes contributed the maximum species (44.9%) followed by Pelecaniformes (9.2%), Charadriiformes (8.2%) and Accipitriformes (6.1%). The species such as the Asian Paradise Flycatcher, Common Hoopoe, and Pied Kingfisher were observed only once in the study area. The common occurrence of the Near Threatened Malabar Pied Hornbill in Bagayat signifies the ornithological importance of this site. Long-term studies on distribution and abundance are required to prepare a conservation plan for avifauna in Sindhudurg District. The data generated can be considered as baseline data for future conservation and management of existing species.
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Ogwang, Tom, and Frank Vanclay. "Social Impacts of Land Acquisition for Oil and Gas Development in Uganda." Land 8, no. 7 (July 8, 2019): 109. http://dx.doi.org/10.3390/land8070109.
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Uganda’s oil and gas sector has transitioned from the exploration phase to the development phase in preparation for oil production (the operations phase). The extraction, processing, and distribution of oil require a great deal of infrastructure, which demands considerable acquisition of land from communities surrounding project sites. Here, we examine the social impacts of project land acquisition associated with oil production in the Albertine Graben region of Uganda. We specifically consider five major oil related projects that have or will displace people, and we discuss the consequences of this actual or future displacement on the lives and livelihoods of local people. The projects are: Tilenga; Kingfisher; the East African Crude Oil Pipeline; the Kabaale Industrial Park; and the Hoima–Kampala Petroleum Products Pipeline. Our findings reveal both positive and negative outcomes for local communities. People with qualifications have benefited or will benefit from the job opportunities arising from the projects and from the much-needed infrastructure (i.e., roads, health centres, airport) that has been or will be built. However, many people have been displaced, causing food insecurity, the disintegration of social and cultural cohesion, and reduced access to social services. The influx of immigrants has increased tensions because of increasing competition for jobs. Crime and social issues such as prostitution have also increased and are expected to increase.
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Blakey, Andrew, Eugenia Acosta, and Ryan Gariepy. "A Field Comparison of Methods and Data Results Between a Manual And Robotic Remote Sensed Survey of a Storm Water Management Pond in Kitchener, Ontario." GEOMATICA 68, no. 4 (December 2014): 345–54. http://dx.doi.org/10.5623/cig2014-408.
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Conventional shallow water bathymetric survey methods are time consuming, expensive, susceptible to human error, and often hazardous. An innovative end-to-end solution that improves water surveying by reduc ing these risks through the use of an unmanned bathymetric data collection system has been designed and built. This system combines a collection of pre- and post-processing routines with the capabilities of a purposebuilt unmanned surface vessel to measure and build accurate bathymetric datasets that are easy to integrate into any CAD or GIS database. Data collection involves the fusion of high-accuracy data from a single beam echosounder, GPS, and iner tial measurement unit (IMU) to generate sediment surface points at centimetre-level accuracy. Through use of an unmanned vessel, these surveys are repeatable without requiring any persons to be on or in the waterbody. This paper presents the results of a focused validation study conducted to illustrate that this platform gen er ates comparable data to a manual survey while greatly reducing survey time and improving safety. Results show a volumetric difference of 2.8% between the Kingfisher USV and manual survey data. Surveying duration was shorter at 42 minutes compared to 140 minutes. This survey was also reliably completed despite challenging environmental conditions.
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Bogožalec Košir, Alexandra, Dane Lužnik, Viktorija Tomič, and Mojca Milavec. "Evaluation of DNA Extraction Methods for Reliable Quantification of Acinetobacter baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa." Biosensors 13, no. 4 (April 6, 2023): 463. http://dx.doi.org/10.3390/bios13040463.
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Detection and quantification of DNA biomarkers relies heavily on the yield and quality of DNA obtained by extraction from different matrices. Although a large number of studies have compared the yields of different extraction methods, the repeatability and intermediate precision of these methods have been largely overlooked. In the present study, five extraction methods were evaluated, using digital PCR, to determine their efficiency in extracting DNA from three different Gram-negative bacteria in sputum samples. The performance of two automated methods (GXT NA and QuickPick genomic DNA extraction kit, using Arrow and KingFisher Duo automated systems, respectively), two manual kit-based methods (QIAamp DNA mini kit; DNeasy UltraClean microbial kit), and one manual non-kit method (CTAB), was assessed. While GXT NA extraction kit and the CTAB method have the highest DNA yield, they did not meet the strict criteria for repeatability, intermediate precision, and measurement uncertainty for all three studied bacteria. However, due to limited clinical samples, a compromise is necessary, and the GXT NA extraction kit was found to be the method of choice. The study also showed that dPCR allowed for accurate determination of extraction method repeatability, which can help standardize molecular diagnostic approaches. Additionally, the determination of absolute copy numbers facilitated the calculation of measurement uncertainty, which was found to be influenced by the DNA extraction method used.
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Djakic, A. W. "GEOTECHNICAL PROCESSES AND MANAGEMENT STRATEGIES FOR MATURE BASINS: A CASE STUDY OF THE GIPPSLAND BASIN." APPEA Journal 36, no. 1 (1996): 691. http://dx.doi.org/10.1071/aj95049.
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During the 1990s, exploitation and exploration processes in the Gippsland Basin have been expanded to cater to a surge in mature field redevelopment activity, and have supported continued activity in the more traditional functions of exploration and development of new resources.The first major field redevelopment project was initiated in 1991 on the West Kingfish Field. Since 1991, similar programs conducted on the Mackerel, Perch, Fortescue and Kingfish Fields have significantly mitigated oil production decline in the basin. The success of the field redevelopment programs has been attributed to the use of multi-disciplinary field teams, the use of high end geophysical interpretation technologies, and the ability of the field teams to integrate and synthesise often voluminous quantities of production and geotechnical information to identify drilling opportunities.Areas where we continue to improve field redevelopment geotechnical processes include decreasing data acquisition and analysis cycle time, and advancing the precision of geotechnical predictions.With over 30 years of intensive exploration activity in the Gippsland Basin placing it in a mature phase of development, focus on the risk management of exploration activities has increased. Focussed research efforts to both develop new geotechnical technologies and more accurately assess the limitations of currently utilised technologies have also been undertaken to ensure an economically viable exploitation and exploration strategy for the basin.The structure of geotechnical teams in the 1990s has seen production and exploration functions amalgamate to ensure that the benefits of operations synergies provided by the high level of platform drilling activity are fully realised. This is enabling the knowledge from intensive field drilling programs to be more readily applied to the evaluation of exploration opportunities. Field re-development drilling activities are at historically high levels and are predicted to remain so into the foreseeable future. Given the mature nature of exploration in the basin, future exploration activities will be undertaken within the defined limits of our technologies to ensure the opportunity to achieve continued economic success is maximised.
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Hunt, L. K., I. De Looze, M. Boquien, R. Nikutta, A. Rossi, S. Bianchi, D. A. Dale, et al. "Comprehensive comparison of models for spectral energy distributions from 0.1 μm to 1 mm of nearby star-forming galaxies." Astronomy & Astrophysics 621 (January 2019): A51. http://dx.doi.org/10.1051/0004-6361/201834212.
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We have fit the far-ultraviolet (FUV) to sub-millimeter (850 μm) spectral energy distributions (SEDs) of the 61 galaxies from the Key Insights on Nearby Galaxies: A Far-Infrared Survey with Herschel (KINGFISH). The fitting has been performed using three models: the Code for Investigating GALaxy Evolution (CIGALE), the GRAphite-SILicate approach (GRASIL), and the Multiwavelength Analysis of Galaxy PHYSical properties (MAGPHYS). We have analyzed the results of the three codes in terms of the SED shapes, and by comparing the derived quantities with simple “recipes” for stellar mass (Mstar), star-formation rate (SFR), dust mass (Mdust), and monochromatic luminosities. Although the algorithms rely on different assumptions for star-formation history, dust attenuation and dust reprocessing, they all well approximate the observed SEDs and are in generally good agreement for the associated quantities. However, the three codes show very different behavior in the mid-infrared regime: in the 5–10 μm region dominated by PAH emission, and also between 25 and 70 μm where there are no observational constraints for the KINGFISH sample. We find that different algorithms give discordant SFR estimates for galaxies with low specific SFR, and that the standard recipes for calculating FUV absorption overestimate the extinction compared to the SED-fitting results. Results also suggest that assuming a “standard” constant stellar mass-to-light ratio overestimates Mstar relative to the SED fitting, and we provide new SED-based formulations for estimating Mstar from WISE W1 (3.4 μm) luminosities and colors. From a principal component analysis of Mstar, SFR, Mdust, and O/H, we reproduce previous scaling relations among Mstar, SFR, and O/H, and find that Mdust can be predicted to within ∼0.3 dex using only Mstar and SFR.
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Kasimov, Vasilli, Michelle Wille, Subir Sarker, Yalun Dong, Renfu Shao, Clancy Hall, Dominique Potvin, et al. "Unexpected Pathogen Diversity Detected in Australian Avifauna Highlights Potential Biosecurity Challenges." Viruses 15, no. 1 (January 2, 2023): 143. http://dx.doi.org/10.3390/v15010143.
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Birds may act as hosts for numerous pathogens, including members of the family Chlamydiaceae, beak and feather disease virus (BFDV), avipoxviruses, Columbid alphaherpesvirus 1 (CoAHV1) and Psittacid alphaherpesvirus 1 (PsAHV1), all of which are a significant biosecurity concern in Australia. While Chlamydiaceae and BFDV have previously been detected in Australian avian taxa, the prevalence and host range of avipoxviruses, CoAHV1 and PsAHV1 in Australian birds remain undetermined. To better understand the occurrence of these pathogens, we screened 486 wild birds (kingfisher, parrot, pigeon and raptor species) presented to two wildlife hospitals between May 2019 and December 2021. Utilising various qPCR assays, we detected PsAHV1 for the first time in wild Australian birds (37/486; 7.61%), in addition to BFDV (163/468; 33.54%), Chlamydiaceae (98/468; 20.16%), avipoxviruses (46/486; 9.47%) and CoAHV1 (43/486; 8.85%). Phylogenetic analysis revealed that BFDV sequences detected from birds in this study cluster within two predominant superclades, infecting both psittacine and non-psittacine species. However, BFDV disease manifestation was only observed in psittacine species. All Avipoxvirus sequences clustered together and were identical to other global reference strains. Similarly, PsAHV1 sequences from this study were detected from a series of novel hosts (apart from psittacine species) and identical to sequences detected from Brazilian psittacine species, raising significant biosecurity concerns, particularly for endangered parrot recovery programs. Overall, these results highlight the high pathogen diversity in wild Australian birds, the ecology of these pathogens in potential natural reservoirs, and the spillover potential of these pathogens into novel host species in which these agents cause disease.
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Dettleff, Phillip, Javiera Rodríguez, Daniel Patiño-García, Renan Orellana, Rodrigo Castro, Sebastián Escobar-Aguirre, Ricardo Daniel Moreno, and Jaime Palomino. "Molecular Characterization of Embryos with Different Buoyancy Levels in the Yellowtail Kingfish (Seriola lalandi)." Animals 12, no. 6 (March 12, 2022): 720. http://dx.doi.org/10.3390/ani12060720.
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The buoyancy of eggs and embryos is associated with successful development in pelagic fish. Buoyancy is the result of oocyte hydration, which depends on the osmotic force exerted by free amino acids (FAA) generated by yolk proteolysis, and cathepsins are the main enzymes involved in this process. Seriola lalandi is a pelagic fish whose farming has been hampered by development failure that have been partially attributed to decreased buoyancy of embryos. Therefore, the aim of this study was to compare the mRNA expression and activity of cathepsins B, D, and L, as well as the FAA content in floating and low-floating embryos at different developmental stages. The chosen stages were eggs, morula, blastula, gastrula and 24 h embryos. Complementary assessments showed that there were no differences attributed to buoyancy status in embryo and oil droplet diameters, as well as the transcriptional status at any developmental stage. Cathepsin B did not show differences in mRNA expression or activity related to buoyancy at any stage. Cathepsin D displayed higher transcript and activity levels only in low-floating eggs compared with those floating. Cathepsin L showed higher expression in floating eggs and 24 h embryos compared with that of low-floating, but the activity of this enzyme was higher in floating eggs and morula. Total FAA content constantly decreased throughout development in floating embryos, but it was always higher than low-floating embryos until gastrula stage. In 24 h embryos floating and low-floating embryos share similar quantities of FAA. In summary, differences in the expression and activity of cathepsins between floating and low-floating embryos could be revealed at specific embryonic stages, suggesting different functions of these enzymes throughout development. Besides 24 h embryos, FAA content seems to be a decisive factor for buoyancy of embryos during early development of S. lalandi. Overall, considering the main role of cathepsins and FAA in buoyancy acquisition process and therefore in both embryo quality and viability, our study identifies good marker candidates to evaluate embryo quality in the farming of this species.
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Bhusal, Ashika, and Anjana Devkota. "Environmental variables and macrophytes of lakes of the Chitwan National Park, Central Nepal." Limnological Review 20, no. 3 (September 1, 2020): 135–44. http://dx.doi.org/10.2478/limre-2020-0014.
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AbstractWetlands are areas where water is the primary factor controlling the environment, associated plant and animal life. The present study has been conducted to investigate the environmental variables of water and to make a quantitative analysis of aquatic macrophytes. Five lakes, i.e. Tamor, Beeshazar, Kingfisher, Athaieshazar and Chepang of the Chitwan National Park were studied during the post-monsoon season. Four different sites were studied for each lake. 12 replica of water samples were collected from each site of the lakes and environmental variables of water i.e. pH, temperature, dissolved oxygen, total alkalinity, conductivity, total hardness, nitrate, inorganic phosphorus, total solid matter and free CO2 were analyzed. Macrophyte floras were collected during field sampling with the help of a 1m×1m light wooden quadrat and herbaria were prepared following the standard technique. Altogether 10 quadrats (five paired quadrats) were plotted in each site of the study area. Data obtained from water analysis and macrophytes were analyzed by SPSS statistics 20 and CANACO version 4.5. Higher values of nitrate and inorganic phosphorus were found in Beeshazar Lake and Tamor Lake, i.e. 4.41 ±0.52 mg dm–3 and 2.93 ±0.29 mg dm–3, respectively. In most of the lakes, macrophyte species, mostly Eichhornia crassipes was found to be dominant in terms of the importance value index (IVI), whereas in Tamor Lake, Spirodela polyrhiza was dominant. All the studied lakes were in the hypereutrophic category on the basis of nitrate and phosphorus concentration. Runoff of polluted water with agricultural fertilizers, pesticides and untreated industrial discharge as well as domestic wastes from the Khageri canal and excessive growth of invasive macrophytes are major threats to the studied lakes. The luxuriant growth of invasive macrophytes like Eichhornia crassipes also revealed the productive nature of the lakes and they can be taken as ecological indicators of organic pollution.
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Chan, W. L. "(P1-38) Emergency Department Preparedness for Training Management Plan towards Mass Casualty Incidents." Prehospital and Disaster Medicine 26, S1 (May 2011): s110—s111. http://dx.doi.org/10.1017/s1049023x11003700.
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It is important to equip emergency department (ED) staff with skills to manage mass casualty incidents (MCI) as disasters strike without warning. Our hospital, Tan Tock Seng Hospital, has been the national screening centre for severe acute respiratory distress syndrome (SARS) and H1N1 outbreaks in 2003 and 2009. Furthermore, our ED has managed casualties from mass food poisoning in the community. We would like to share our experiences in training our staff for MCI. For the ED to operate smoothly in a MCI, comprehensive training of staff during “peace” time is essential. We have a selected team of doctors and nurses as the department disaster workgroup. This team, together with the hospital emergency planning department, prepare the disaster protocols using an “all hazard approach concept” and aim to minimise variations between different protocols (Conventional, Infectious disease, Hazmat, Radioactive MCI). These protocols are updated regularly, with new information disseminated to all staff. Next, all staff must be well-versed in the protocols. New staffs undergo orientation programmes to familiarize them with the work processes. Regular audits are conducted to ensure that the quality is well-maintained. Additionally, training also occurs at the inter-departmental and national levels. There are regular activation exercises to test inter-departmental response to MCI and collaborations with Ministry of Health to conduct disaster exercises e.g. the biennial Kingfisher Exercise in preparation for radiation-related MCI. Such exercises improve communication and working relationships within the ED and with other departments. The camaraderie developed can act as a pillar of support during stressful times of MCI. Lastly, the ED staffs attend local and international courses and conferences to update ourselves on the latest training and knowledge in the handling of MCI. This allows us to share our ideas and to learn from our local and international counterparts, and helps better prepare ourselves.
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Sutter, Jessica, Daniel A. Dale, Karin Sandstrom, J. D. T. Smith, Alberto Bolatto, Mederic Boquien, Daniela Calzetti, et al. "The case for thermalization as a contributor to the [C ii] deficit." Monthly Notices of the Royal Astronomical Society 503, no. 1 (February 20, 2021): 911–19. http://dx.doi.org/10.1093/mnras/stab490.
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ABSTRACT The [C ii] deficit, which describes the observed decrease in the ratio of [C ii] 158 μm emission to continuum infrared emission in galaxies with high star formation surface densities, places a significant challenge to the interpretation of [C ii] detections from across the observable universe. In an attempt to further decode the cause of the [C ii] deficit, the [C ii] and dust continuum emission from 18 Local Volume galaxies has been split based on conditions within the interstellar medium where it originated. This is completed using the Key Insights in Nearby Galaxies: a Far-Infrared Survey with Herschel (KINGFISH) and Beyond the Peak (BtP) surveys and the wide-range of wavelength information, from UV to far-infrared emission lines, available for a selection of star-forming regions within these samples. By comparing these subdivided [C ii] emissions to isolated infrared emission and other properties, we find that the thermalization (collisional de-excitation) of the [C ii] line in H ii regions plays a significant role in the deficit observed in our sample.
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Escher, Claudia, Jakob Vowinckel, Karel Novy, Thomas Corwin, Tobias Treiber, Roland Bruderer, Lukas Reiter, Eike-Christin von Leitner, and Oliver Rinner. "Next generation proteomics in precision oncology: 1000s of proteome and phosphoproteome profiles of tumors and matching healthy tissues as meaningful layer in multi-omics database." Journal of Clinical Oncology 38, no. 15_suppl (May 20, 2020): e15672-e15672. http://dx.doi.org/10.1200/jco.2020.38.15_suppl.e15672.
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e15672 Background: The rise of precision oncology therapeutics requires deep understanding of all molecular mechanisms involved in cancer biology. IndivuType offers the world’s first multi-omics database for individualized cancer therapy, analyzing the highest quality cancer biospecimens to generate the most comprehensive dataset, including genomics (WGS), transcriptomics, proteomics, and clinical outcome information. Indivumed is committed to the quality of the IndivuType ecosystem starting with stringent SOP-driven sample collection combined with thorough validation of clinical information and data integrity. The availability of multi-omics data from the same tumor can provide a comprehensive molecular picture of cancer for a given patient. Protein expression and activation are directly related to cellular function and hence provide actionable information about druggable targets. Until recently, the proteomics technology could not match the scale of next-gen sequencing and consequently precision medicine has almost exclusively been based on gene level data. Here we present the first large-scale data set for protein expression and phosphorylation. Enabled by the data independent acquisition (DIA) workflow, a mass spectrometric method provided by Biognosys that obtains peptide fragmentation data in a highly parallelized way with high sensitivity, more than 7,000 proteins in the whole proteome (WP) and 20,000 phospho-peptides in the phospho-proteome (PP) workflow were profiled. Methods: Sample processing from 5 mg of tissue per sample was performed using liquid handling robot. Phospho-peptide enrichment was carried out with a Kingfisher Flex device and MagReSyn Ti-IMAC magnetic beads. DIA LC-MS/MS was performed on multiple platforms consisting of a Thermo Scientific Q Exactive HF-X mass spectrometer coupled to a Waters M-Class LC. Chromatography was operating at 5 µL/min, and separation was achieved using 45 min (WP) and 60 min (PP) gradients. Results: Several thousands of high-quality patient samples of various cancer types have been analyzed to date. The resulting proteome and phospho-proteome data has been integrated into the IndivuType database, thereby providing a solid foundation to advance our understanding of cancer. Conclusions: With the ongoing addition of more samples and associated deep and rich data, the platform could unravel key molecular events and is expected to transform knowledge into actionable treatments and personalized therapies.
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Ramos-Martínez, E., O. G. Morales-González, J. A. Ramírez, F. L. García-Carreño, and J. G. Montejano-Gaitán. "Determinación de la presencia y el origen del fenómeno modori en surimi de cinco especies de pescado del Golfo de México / Determination of the modori phenomenon and its origin in surimi of five fish species from the Gulf of Mexico." Food Science and Technology International 5, no. 5 (October 1999): 397–405. http://dx.doi.org/10.1177/108201329900500505.
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Modori is a phenomenon which occurs in fish gels only when they are incubated at 50-70 °C, and which induces a decrease in their mechanical properties. Modori has been associated with endogenous proteases, varying among fish species. Surimis were obtained from Atlantic croaker ( Micropogon undulatus) , barred grunt ( Conodon nobilis) , sole ( Cyclopsetta chittendeni) , northern kingfish ( Menticirrhus saxatilis) and striped searobin ( Prionotus evolans). The effect of gel incubation time on shear stress and shear strain at failure, soluble peptides and electrophoretic pattern was studied. Incubation times at 60 °C, of 0, 20 or 40 min were assayed. The modori phenomenon induced a significant decrease (25-50%) in the mechanical properties of the five species studied. This phenomenon was associated with the presence of active proteases at 60 °C, determined as an increase in concentration of soluble pep tides depending on the time of incubation. Proteases were partially characterized employing serine- and cysteine-protease inhibitors. The studies with SDS-PAGE showed that myosin is the main substrate of the proteases. Actin was not hydrolyzed. The employment of specific inhibitors showed that both serine- and cysteine-proteases were associated with the modori phenomenon in the species studied.
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Bozzolo, Arianna, Andrew L. Thomas, Jackie L. Harris, Connie Liu, Misha Kwasniewski, and R. Keith Striegler. "Performance of ‘Chambourcin’ Winegrape on 10 Different Root Systems in Southern Missouri, USA." HortTechnology 33, no. 3 (June 2023): 253–61. http://dx.doi.org/10.21273/horttech05163-22.
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In the midwestern United States, especially Missouri, winegrape (Vitis sp.) growers mostly plant interspecific hybrids, which are well adapted to the climate and pests of the region. ‘Chambourcin’ (an interspecific French-American hybrid) is one of the most widely planted winegrape cultivars in the area. It is usually grown as own-rooted (nongrafted) vines because the economic and horticultural benefits of grafting this cultivar to rootstocks have not been well developed. Further, few significant winegrape rootstock evaluations have been conducted in the midwestern United States, including evaluations of newer rootstocks developed and released by private and public breeding programs. The aim of this study was to assess the potential value of using rootstocks in ‘Chambourcin’ production in southern Missouri, with implications for the midwestern United States. Fruit yield, vine growth, and fruit composition metrics from ‘Chambourcin’ on 10 different root systems [own-rooted, and grafted to rootstocks ‘Couderc 3309’, ‘Couderc 1616’, ‘Paulsen 1103’, ‘Sélection Oppenheim 4’, ‘Millardet et de Grasset 420A’, ‘Millardet et de Grasset 101-14’, ‘Kingfisher’, ‘Matador’ (all Vitis sp.), and ‘Gloire de Montpellier’ riverbank grape (Vitis riparia)] in an experimental vineyard in southwest Missouri were compared. Following three establishment years (2008–10), data were collected across four growing and vintage seasons (2011–14). Yield components evaluated included total fruit production, clusters per vine, cluster weight, berry weight, weight of cane prunings, and crop load. Petiole mineral analysis was conducted in 2011, 2013, and 2014. Grape juice attributes measured were soluble solids concentration, juice pH, titratable acidity (TA), potassium (K), anthocyanins, tannins, phenolics, and organic acids. When simply comparing grafted vs. ungrafted vines, grafting generally induced higher plant vigor and a higher pH in the juice, whereas the other parameters did not differ. When the performances were compared among the 10 root systems, vines grafted to ‘Couderc 3309’ had higher yields compared with vines grafted to six other rootstocks and own-rooted vines. Grafting to ‘Millardet et de Grasset 101-14’ induced higher cluster weight compared with the other rootstocks. The ‘Millardet et de Grasset 420A’ rootstock promoted a higher pH and TA as well as a higher concentration of K in the juice, and ‘Paulsen 1103’ also promoted high pH, TA, and malic acid in the juice, and higher concentrations of phosphorous (P) and K in the petiole compared with most rootstocks. ‘Gloire de Montpellier’ induced a lower P content in the petiole and a higher tartaric/malic acid ratio. Rootstock use can strongly influence some vineyard production metrics as well as nutrient uptake and K levels in the juice (the latter further influencing juice pH). The results of this study provide insights into the complex viticultural and enological interactions resulting from the use of rootstocks in hybrid winegrape production in Missouri, USA.
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Perez-Egusquiza, Z., L. W. Liefting, and L. I. Ward. "First Report of Rose yellow vein virus in Rosa sp. in New Zealand." Plant Disease 97, no. 8 (August 2013): 1122. http://dx.doi.org/10.1094/pdis-10-12-0981-pdn.
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Rose is the top selling cut flower in New Zealand and is the most popular garden plant in the world. Several virus-like diseases have been described in roses, but the causal agents for many remain unknown. Most of the described viruses infecting rose belong to the genera Ilarvirus and Nepovirus. Only recently, a number of new viruses have been or are in the process of being characterized (1,2,3,4). In January 2011, 10 rose samples showing virus-like symptoms were collected from the Wanganui region on the North Island of New Zealand. Total nucleic acid was extracted from these samples using an InviMag Plant DNA Mini Kit (Invitek GmbH, Berlin, Germany) and a KingFisher mL workstation (Thermo Scientific, Waltham, MA). PCR and reverse transcription (RT)-PCR was conducted using specific primers for Arabis mosaic virus (ArMV), Cherry leaf roll virus, Prunus necrotic ringspot virus (PNRSV), Rosa rugosa leaf distortion virus, Rose spring dwarf associated virus, Rose yellow leaf virus, Rose yellow mosaic virus, Rose yellow vein virus (RYVV), and Strawberry latent ringspot virus. Samples were also tested using generic primers for carlavirus, potexvirus, potyvirus, tombusvirus, and phytoplasmas. Two samples (cvs. Pauls Himalayan Musk and Bloomfield) were positive for ArMV, four samples (cvs. Leda, Rosa Mundi, Charles de Mills, and Indica Major) were positive for PNRSV, and two samples (cvs. Leda and Zephirine Drouhin) were positive for RYVV. Samples were negative for all other tested viruses and phytoplasmas. RYVV was detected using two sets of primers (D. Mollov, personal communication) designed to amplify fragments of estimated sizes of 797 bp and 684 bp of the movement protein (MP) and coat protein (CP) genes of RYVV, respectively. RYVV amplicons were sequenced directly (GenBank Accession Nos. JX887423 to JX887426). A BLASTn search of the MP and CP fragments showed the highest nucleotide identity of 98% and 96 to 97%, respectively, with the type isolate of RYVV (JX028536). RYVV has been reported as the causal agent of a vein yellowing disease in rose (2). Symptoms observed in the ‘Leda’ sample infected with PNRSV and RYVV (vein yellowing and chlorotic mottle in the apex of leaves) were not typical of PNRSV, so they may be caused by RYVV. Symptoms in samples of cv. Zephirine Drouhin (curling of leaves and mottle), observed in both RYVV-positive and -negative samples, may not be associated with RYVV infection. This suggests that vein yellowing may be influenced by cultivar. RYVV has been reported in several rose cultivars, but only in the United States (2). To the best of our knowledge, this is the first report of RYVV infecting rose in New Zealand, where it is likely that the virus has been present for some time. The virus may have a much wider geographical distribution than that reported as the virus was only recently characterized (3). References: (1) B. Lockhart et al. Page 31 in: Program and Abstracts of The 12th International Symposium on Virus Diseases of Ornamental Plants, 2008. (2) D. Mollov et al. Phytopathology 99:S87, 2009. (3) D. Mollov et al. Arch Virol. 158:877, 2012. (4) N. Salem et al. Plant Dis. 92:508, 2008.
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Veerakone, S., L. W. Liefting, B. S. M. Lebas, and L. Ward. "First Report of Cherry leaf roll virus in Hydrangea macrophylla." Plant Disease 96, no. 3 (March 2012): 463. http://dx.doi.org/10.1094/pdis-08-11-0708.
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Hydrangea is a popular, summer flowering, ornamental shrub that is native to south and east Asia and North and South America, which is now cultivated throughout the world. Currently, 13 viruses belonging to eight genera have been reported in Hydrangea spp. (1). In April 2011, virus-like disease symptoms, including severe leaf deformation and chlorosis, were observed on two Hydrangea macrophylla ‘Sumiko’ plants from Australia being held in quarantine in New Zealand. Systemic symptoms of veinal necrosis, necrotic halo spots, and severe leaf deformation were observed on Nicotiana occidentalis ‘37B’ 7 days after inoculation with sap from the symptomatic hydrangea plants. Upon reinoculation with sap of symptomatic leaves from N. occidentalis, necrotic ringspots and tip necrosis, typical of nepovirus infection, were observed on leaves of N. tobacum and Chenopodium quinoa, respectively. Transmission electron microscopy of negatively stained sap from symptomatic leaves of N. occidentalis revealed the presence of isometric particles ~28 nm in diameter. Total nucleic acid was extracted from the symptomatic leaves of N. occidentalis with an InviMag Plant DNA Mini Kit (Invitek GmbH, Berlin, Germany) and a KingFisher mL workstation (Thermo Scientific, Waltham, MA). Reverse transcription (RT)-PCR using the reverse primer of Werner et al. (2) and a forward primer, 5′-CGGTGGAGATGCCGGTCCTA-3′ (this study), specific to the 3′-untranslated region (3′-UTR) of Cherry leaf roll virus(CLRV) produced an amplicon of ~1,150 bp from N. occidentalis. A consensus sequence of 1,140 bp generated from four clones of the PCR product (GenBank Accession No. JN418885) was 99 and 98% identical at the nucleotide level to a CLRV isolate from Rumex AGBC (GenBank No. AB168099) and Chinese chives (GenBank No. AB168098), respectively. N. occidentalis also tested positive for CLRV using polyclonal antiserum in a double antibody sandwich-ELISA (BIOREBA, Reinach, Switzerland). The presence of CLRV in the original samples and N. occidentalis was confirmed by direct sequencing of the 380-bp amplicons obtained by immunocapture RT-PCR using CLRV-specific primers (2) and the same antiserum. BLASTn analysis of these amplicons (data not submitted to GenBank) also showed 99% nucleotide identity to a New Zealand isolate from a Rubus sp. (GenBank No. AJ877162). The hydrangea plants were released from quarantine because the same strain of CLRV had previously been reported in New Zealand. To our knowledge, this is the first report of CLRV in hydrangea. CLRV is a seed and pollenborne nepovirus and can be transmitted mechanically and by grafting. Since hydrangeas are mainly vegetatively propagated and are less commonly grown from seeds, the natural spread of CLRV will depend on the movement of infected propagation material. It is unknown whether this virus causes reduction in flower quality in hydrangea as reported in other hosts but any impact on flower quality may be of economic significance in commercial nurseries. References: (1) M. Caballero et al. Plant Dis. 93:891, 2009. (2) R. Werner et al. Eur. J. For. Pathol. 27:309, 1997.
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Gilbert, Michael B., and Kathy A. Hill. "GIPPSLAND, A COMPOSITE BASIN-A CASE STUDY FROM THE OFFSHORE NORTHERN STRZELECKI TERRACE, GIPPSLAND BASIN, AUSTRALIA." APPEA Journal 34, no. 1 (1994): 495. http://dx.doi.org/10.1071/aj93040.
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Detailed interpretation of reflection seismic and well data from the northern Strzelecki Terrace constrain the effect of Southern Margin and Tasman Sea rifting on the evolution of the Gippsland Basin. A new model is proposed which divides the basin into two structurally distinct provinces (East and West Gippsland Basin), separated by a broad zone of accommodation which is referred to in this paper as the 'Kingfish/Tuna Transition Zone'. This zone is a distinct region across which structural styles change within the basin due to the interaction of extensional forces resulting from both Southern Margin and Tasman Sea rifting. No evidence has been found, however, for the existence of transfer zones within the northern margin of Gippsland Basin as previously suggested by other authors.The Gippsland Basin is observed to have a composite history; a younger 'Tasman Rift' Basin (a Tasman Sea aulacogen) overlying a regionally more extensive 'Strzelecki Basin' (the result of rifting along Australia's Southern Margin). Both basins have formed as half graben with opposing asymmetry. Re-evaluation of the Cretaceous palynology in conjunction with reflection seismic data from selected wells have enabled division of the Cretaceous section of the northern Strzelecki Terrace into three tectonically distinct sedimentary units: the Lower Strzelecki, Upper Strzelecki and Golden Beach Megasequences. The Lower Strzelecki Megasequence exhibits considerable thickening towards a south-bounding master fault, and is inferred to have been deposited during a phase of active rifting. It is separated from the overlying Upper Strzelecki Megasequence by a pronounced late Aptian age angular unconformity. The Upper Strzelecki Megasequence is a thick sedimentary unit which shows less syn-sedimentary faulting and is inferred to be deposited during a period of tectonic quiescence, possibly during a sag phase following active rifting. The Golden Beach Megasequence shows renewal of rifting with growth towards a north bounding fault system and is differentiated from the underlying Strzelecki Megasequences by a distinct change in seismic character across a subtle early Campanian age angular unconformity.
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Quemin, M. F., B. S. M. Lebas, S. Veerakone, S. J. Harper, G. R. G. Clover, and T. E. Dawson. "First Molecular Evidence of Citrus psorosis virus and Citrus viroid III from Citrus spp. in New Zealand." Plant Disease 95, no. 6 (June 2011): 775. http://dx.doi.org/10.1094/pdis-12-10-0881.
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In December 2008, a collection of Citrus spp. in Kerikeri, New Zealand was surveyed for virus and viroid diseases. Symptoms characteristic of virus or viroid infection were not observed other than Citrus tristeza virus (CTV)-associated stem pitting when examined with the bark removed. Total RNA was extracted from bark samples of 273 trees using RLT buffer (Qiagen Inc., Chatsworth, CA) on a KingFisher mL workstation (Thermo Scientific, Waltham, MA) and tested by reverse transcription (RT)-PCR). Samples from three trees, two from sweet orange, Citrus × sinensis (L.) Osbeck (pro sp.) (maxima × reticulate) and one from tangerine, Citrus reticulata Blanco, tested positive for Citrus psorosis virus (CPsV), and two samples, one each from lemon, Citrus × limon (L.) Burm. F. (pro sp.) (medica × aurantifolia) and sweet orange, tested positive for Citrus viroid III (CVd-III) using previously published primers and PCR cycling conditions (2,4) in a one-step RT-PCR system. The 20-μl RT-PCR reaction was done with Verso Reddymix reagents (Thermo Scientific) containing 250 nM of specific primers and 300 μg/μl of bovine serum albumin (Sigma-Aldrich, St. Louis, MO). The CVd-III genome was completed using specific internal primers (forward: 5′-AACGCAGAGAGGGAAAGGGAA-3′, reverse: 5′-TAGGGCTACTTCCCGTGGTC-3′) with the following cycling conditions: 50°C for 15 min, 94°C for 2 min, then 40 cycles of 94°C for 10 s, 57°C for 30 s, and 68°C for 30 s. The three CPsV amplicons of 419 bp from the RNA-dependent RNA polymerase gene (GenBank Accession Nos. GQ388241 to GQ388243) had 96 to 100% nucleotide identity to each other. A 276-bp (nt position 48 to 323) fragment of the 419-bp sequence was used for comparison with sequences available on GenBank. The three 276-bp CPsV sequences had 89 to 97% nucleotide identity to other CPsV available in GenBank at the time of the analysis. The CVd-III genomes of 291 bp (GenBank Accession Nos. HQ219183 and JF521494) are identical and showed 94 to 99% nucleotide identity to other CVd-III available in GenBank. The presence of CPsV was confirmed in the three samples by a CPsV-specific double-antibody sandwich-ELISA kit (Agritest S.r.l., Valenzano, Italy), while the presence of CVd-III was confirmed only in the lemon sample by r-PAGE (3). The concentration of the viroid in the sweet orange sample may have been below the detection limit of the test. The incidence of the diseases is probably low since CPsV and CVd-III were detected in only a few trees which were planted between 1998 and 2002 at Kerikeri from budwoods of unknown sources imported between the 1970s and 1990s. New Zealand's growing conditions generally do not favor viroid replication in plants, whereas the temperatures may be suitable for CPsV disease. However, symptom characteristics to CPsV and CVd-III have never been observed on the infected trees. This is most likely because of the presence of CTV in the trees (data not shown). CPsV symptoms were thought to have been observed in the 1950s in New Zealand (1) but the causal agent had not been identified. To our knowledge, this is the first molecular and serological evidence of CPsV and the first report of the presence of CVd-III in New Zealand. References: (1) W. A. Fletcher. Orchard. N. Z. 30:33, 1957. (2) T. Ito et al. J. Virol. Methods 106:235, 2002. (3) C. Jeffries and C. James. OEPP/EPPO Bull. 35:125, 2005. (4) S. Martin et al. J. Gen. Virol. 87:3097, 2006.
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Liefting, L. W., Z. Perez-Egusquiza, and G. R. G. Clover. "First Report of Helleborus net necrosis virus in Hellebore in New Zealand." Plant Disease 94, no. 4 (April 2010): 479. http://dx.doi.org/10.1094/pdis-94-4-0479b.
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Hellebores (Helleborus spp.) are widely grown in gardens for their winter and early spring flowers. They are extremely hardy and will grow easily in many different environments. In April 2009, black streaks on the leaves and stems were observed on approximately 3 to 5% of hellebores in a home garden in the Waikato Region, New Zealand. The symptoms appeared similar to those of ‘black death’, determined to be associated with a newly characterized carlavirus termed Helleborus net necrosis virus (HeNNV) (1). Transmission electron microscopy revealed the presence of typical carlavirus-like particles (slightly flexuous filaments ≈700 nm long) in crude sap extracts. Total nucleic acid was extracted separately from the leaves and stem from one of the symptomatic plants with an InviMag Plant DNA Mini Kit (Invitek GmbH, Berlin, Germany) and a KingFisher mL workstation (Thermo Scientific, Waltham, MA). One-step reverse transcription (RT)-PCR using carlavirus group-specific primers (Agdia Inc., Elkhart, IN) produced an amplicon of approximately 300 bp in both the leaf and stem samples. The PCR product was cloned into the pCR4-TOPO vector (Invitrogen, Carlsbad, CA) and three clones were sequenced. BLAST analysis of the consensus sequence (GenBank Accession No. GQ499837) showed the highest nucleotide identity (78%) to the replicase polyprotein genes of HeNNV strains I6 and G5 (GenBank Accession Nos. FJ196837 and FJ196836, respectively). A fragment of 376 bp was also amplified from the symptomatic plant by RT-PCR with primers HCV8484c and HCV8109, designed specifically to amplify the capsid protein genes and putative nucleic acid binding protein genes of HeNNV (1) and sequenced directly (GenBank Accession No. GQ499838). BLAST analysis showed the highest nucleotide identity (85%) with HeNNV strain G5 (GenBank Accession No. FJ196835) followed by 84% nucleotide identity with HeNNV strains H6 and I6 (GenBank Accession Nos. FJ196836 and FJ196837, respectively). Three asymptomatic hellebore plants purchased from a nursery tested negative by RT-PCR using the carlavirus group-specific and HeNNV-specific primers as described above. To our knowledge, this is the first report of HeNNV infecting hellebores in New Zealand. Hellebores are regularly imported into New Zealand as tissue cultures or nursery stock. The import requirement for acceptance of plants in tissue culture is visual inspection at the border. Nursery stock must be grown in a post-entry quarantine facility for 6 months during which time they are inspected for pests and diseases. The long latent period of HeNNV (1) and the recent discovery of the etiology of ‘black death’ could have resulted in accidental introduction of diseased plants. Knowledge of the causal agent of ‘black death’ is beneficial to growers who have already implemented cultural control strategies to reduce the spread of the disease. Most carlaviruses are aphid transmitted but no vector of HeNNV has been identified, and therefore, it is unknown if the virus spreads naturally in New Zealand. Reference: (1) K. C. Eastwell et al. Plant Dis. 93:332, 2009.
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Sepúlveda, Fabiola A., Luis A. Ñacari, and Maria Teresa González. "First Report of Blood Fluke Pathogens with Potential Risk for Emerging Yellowtail Kingfish (Seriola lalandi) Aquaculture on the Chilean Coast, with Descriptions of Two New Species of Paradeontacylix (Aporocotylidae)." Pathogens 10, no. 7 (July 6, 2021): 849. http://dx.doi.org/10.3390/pathogens10070849.
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Blood flukes are digeneans that infect wild and farmed fish that can cause a severe and potentially lethal disease in farmed fish. These parasites are undetectable in the larval stage based on macroscopic observations in the definitive host with the infection becoming evident when eggs accumulate in the branchial vessels. There are nine known species of the genus Paradeontacylix and seven exclusively parasitize Seriola spp. from several geographical areas. Seriola lalandi aquaculture farms are emerging at various localities in northern Chile. Here, we report, for the first time, two blood fluke species parasitizing S. lalandi in the Southeastern Pacific (Chile). In the laboratory, the gills and heart of fish were removed. The retained blood flukes were separated according to the infection site, fixed in 70% or 95% ethanol for taxonomic and molecular analysis, respectively. Morphometrical differences among the fluke species were evaluated with a principal component analysis (PCA) using proportional body measurements. Phylogenetic trees were constructed based on 28S rDNA, cox1 mDNA using Bayesian inference (BI), and maximum likelihood (ML). Based on morphology, morphometry, and molecular analyses, two new species are proposed: P. humboldti n. sp. from the gills and P. olivai n. sp. from the heart of S. lalandi. Both were clearly distinguished from other species of Paradeontacylix by a combination of morphologic features (posterior tegumental spines, testes arrangement, body size). The genetic distance (based on cox1) among species was >10%. P. humboldti n. sp. and P. olivai n. sp. are sister species (with a common ancestor) independent of P. godfreyi from S. lalandi in Australia. The newly identified parasites may pose a risk to farmed S. lalandi as aporocotylids have been the cause of diseases in farmed fish from other geographical areas. In addition, some cages of S. lalandi are currently maintained in an open circulating system, which could favor the transmission of these parasites (if involved hosts are present in the environment).
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Story, I. "THE AUSTRALIAN OIL INDUSTRY — TWO YEARS OF FLOOD BEFORE THE PERMANENT DROUGHT." APPEA Journal 26, no. 1 (1986): 106. http://dx.doi.org/10.1071/aj85012.
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World oil intensities have undergone a fundamental change since 1976, when gross domestic product growth outstripped oil consumption for the first time. World oil demand has fallen each year since 1979 irrespective of economic conditions.International supply management is the only way of containing oil price falls. In the longer term, non-OPEC production will peak by the end of this decade because there have been no major developments since the mid-1970's. Prices are likely to rise again as supply from non-OPEC countries falls, allowing OPEC to reassert market control.Australia has achieved 100 per cent self-sufficiency in crude oil, but a substantial increase in exploration activity is required if a major fall in self-sufficiency is to be averted after Bass Strait peaks in 1987. Browse Basin (Timor Sea) disappointments indicate that while there will be good production from Jabiru and perhaps Challis, these will not replace the declining Mackerel, Halibut, and Kingfish fields in Bass Strait.The Cooper/Eromanga Basin, while highly prospective, will never produce the quantities needed to replace Bass Strait. Jackson's reserves are estimated at about 45 million barrels. By comparison, Mackerel and Halibut together will produce 58 million barrels in 1985-86 alone.If oil is not found the balance of payments will suffer badly. Each percentage point drop in self sufficiency will cost Australia $85 million or nearly 0.5 per cent of exports. If domestic production falls to 470 000 barrels per day by 1990, imports of crude oil will cost $2 billion in 1985 dollars (assuming flat oil prices). Expressed in another way, 6 per cent of Australia's exports will be required to pay for the incremental drop in self-sufficiency by 1990.In 1984-85 the Government took in $4.26 billion from the crude oil levy, almost exclusively from Bass Strait. In 1985-86 the Government will receive $4.7 billion. This represents 8.7 per cent of Government taxation revenue, and 8 per cent of total Government receipts. By 1990, the levy from Bass Strait will fall by 45 per cent (assuming a constant oil price). By 1995 the revenue will be 90 per cent less than 1985-86, posing a major budgetary funding problem.
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Liefting, L. W., S. Veerakone, L. I. Ward, and G. R. G. Clover. "First Report of ‘Candidatus Phytoplasma australiense’ in Potato." Plant Disease 93, no. 9 (September 2009): 969. http://dx.doi.org/10.1094/pdis-93-9-0969a.
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In January of 2009, potato plants (Solanum tuberosum) from a commercial crop in the Waikato Region, New Zealand were observed to have symptoms of upward rolling and purpling of the leaves. The symptoms appeared similar to those of “zebra chip”, a disorder of potato recently found to be associated with ‘Candidatus Liberibacter solanacearum’ in New Zealand and the United States (4). Total DNA from the leaf midveins and tubers from one of the symptomatic plants was separately extracted with an InviMag Plant DNA Mini Kit (Invitek GmbH, Berlin, Germany) and a KingFisher mL workstation (Thermo Scientific, Waltham, MA). DNA extracted from leaf midveins and tubers tested negative for ‘Ca. L. solanacearum’ by nested-PCR using primer pair OA2/OI2c (4) followed by Lib16S01F/Lib16S01R (5′-TTCTACGGGATAACGCACGG-3′ and 5′-CGTCAGTATCAGGCCAGTGAG-3′), which amplifies a 580-bp region of the 16S rRNA gene. However, DNA extracted from the tuber tissue tested positive for phytoplasma by TaqMan real-time PCR (3). No phytoplasma was detected in the DNA extracted from leaf tissue. The 16S rRNA gene, 16S-23S rRNA intergenic spacer region, and part of the 23S rRNA gene of the phytoplasma were amplified with primers P1/P7 (1). The PCR product was cloned into the pCR 4-TOPO vector (Invitrogen, Carlsbad, CA) and sequenced (GenBank Accession No. FJ943262). BLAST analysis showed 100% identity to ‘Ca. Phytoplasma australiense’ (16SrXII, Stolbur group). A fragment of approximately 850-bp of the Tuf gene was also amplified (2) and sequenced directly (GenBank Accession No. FJ943263). BLAST analysis showed 100% identity to Tuf gene variant IX of ‘Ca. P. australiense’ (2). An additional 14 plants showing similar leaf symptoms and also production of aerial tubers were collected from seven different potato fields from the Auckland and Waikato regions. Total DNA from the leaf midveins, stem, and tubers were separately extracted from each of the plants. The samples were tested for phytoplasma by nested-PCR using primer pair R16F2/R16R2, followed by NGF/NGR (1), and tested for ‘Ca. L. solanacearum’ by nested-PCR as described above. Seven plants tested positive only for phytoplasma, three tested positive for only ‘Ca. L. solanacearum’, and four plants tested positive for both pathogens. The pathogens were most commonly detected in samples extracted from the stem with 9 and 5 of the 14 samples testing positive for phytoplasma and liberibacter, respectively. Six of each of the leaf and tuber samples tested positive for phytoplasma. Liberibacter was detected in one of the leaf samples and in four of the tuber samples. ‘Ca. P. australiense’ has only been reported from New Zealand and Australia. The only other known hosts of ‘Ca. P. australiense’ in New Zealand are strawberry and native plants belonging to the genera Cordyline, Coprosma, and Phormium (2). In Australia, ‘Ca. P. australiense’ is associated with Australian grapevine yellows and Papaya dieback (2). To our knowledge, this is the first report of ‘Ca. P. australiense’ infecting potato as well as the first report of phytoplasma and ‘Ca. L. solanacearum’ mixed infections in potato. References: (1) M. T. Andersen et al. Plant Pathol. 47:188, 1998. (2) M. T. Andersen et al. Phytopathology 96:838, 2006. (3) N. M. Christensen et al. Mol. Plant Microbe Interact. 17:1175, 2004. (4) L. W. Liefting et al. Plant Dis. 93:208, 2009.
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Cowley, R., and G. W. O'Brien. "IDENTIFICATION AND INTERPRETATION OF LEAKING HYDROCARBONS USING SEISMIC DATA:A COMPARATIVE MONTAGE OF EXAMPLES FROM THE MAJOR FIELDS IN AUSTRALIA'S NORTHWEST SHELF AND GIPPSLAND BASIN." APPEA Journal 40, no. 1 (2000): 119. http://dx.doi.org/10.1071/aj99008.
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An extensive volume of 3D seismic data over a number of oil and gas fields in Australia's North West Shelf and Gippsland Basin has been examined for evidence of the effects of hydrocarbon migration and/or leakage. For comparative purposes, 2D and 3D data have also been studied over a number of adjacent traps, including dry traps and partially to completely breached accumulations. Fields and traps investigated include Bayu-Undan, Jabiru, Skua, Swift and Tahbilk in the Bonaparte Basin, Cornea in the Browse Basin, North Rankin, Chinook, Macedon, Enfield and Zeewulf in the Carnarvon Basin, and Kingfish in the Gippsland Basin. The principal goal of the study is to provide representative case studies from known fields so that, in undrilled regions, the exploration uncertainties associated with the issues of hydrocarbon charge and trap integrity might be reduced.Direct indicators of hydrocarbon migration and/or leakage are relatively rare throughout the basins studied, though the discoveries themselves characteristically show seismic anomalies attributable to hydrocarbon leakage. The nature and intensity of these hydrocarbon-related seismic effects do, however, vary dramatically between the fields. Over traps such as Skua, Swift, Tahbilk and Macedon, they are intense, whereas over others, for example Chinook and North Rankin, they are quite subtle. Hydrocarbon-related diagenetic zones (HRDZs), which had been identified previously above the reservoir zones of leaky traps within the Bonaparte Basin, have also been recognised within the Browse, Carnarvon, Otway and Gippsland Basins. HRDZs are the most common leakage indicators found and are identified easily via a combination of high seismic amplitudes through the affected zone, time pull-up and degraded stack response of underlying reflectors. In some cases (the Skua and Macedon Fields), the HRDZs actually define the extent of the accumulations at depth.Anomalous, subtle to strong, seismic amplitude anomalies are associated with the majority of the major fields within the Carnarvon Basin. The strength and location of the anomalies are related to a complex interplay between trap type (in particular four-way dip-closed versus fault dependent), top seal capacity, fault seal integrity, and charge history. In some areas within the Carnarvon, Browse and Bonaparte Basins, shallow amplitude anomalies can be related directly to gas chimneys emanating from the reservoir zone itself. In other instances, the continuous migration of gas from the reservoir has produced an assortment of pockmarks, mounds and amplitude anomalies on the present day sea floor, which all provide evidence of hydrocarbon seepage. In the Browse Basin, strong evidence has been found that many of the modern carbonate banks and reefs in the region were initially located over hydrocarbon seeps on the palaeo-seafloor.The examples and processes presented demonstrate that the analysis of hydrocarbon leakage indicators on seismic data can help to better understand exploration risk and locate subtle hydrocarbon accumulations. In mature exploration provinces, this methodology may lead to the identification of subtle accumulations previously left undetected by more conventional methods. In frontier regions, it can help to identify the presence of a viable petroleum system, typically the principal exploration uncertainty in undrilled regions.
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Ramanchi, Radhika, Sunita Mehta, and Madhavi Vedera. "Equity research and valuation: Jet Airways." Emerald Emerging Markets Case Studies 7, no. 2 (June 5, 2017): 1–28. http://dx.doi.org/10.1108/eemcs-06-2016-0144.
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Subject area This case helps students to analyze non-financial and financial aspects of a company and observe quantitative and qualitative aspects of decisions and decide whether to invest or not and give suggestions to sell, buy or hold stocks. The case is expected to help the students understand and analyze the following points: the overall performance of the company and industry, how fundamental and technical analysis is applied to reach investment decisions, the areas where Jet Airways occupies the top position compared to peer group (competitor analysis), the company’s financial position and valuation with the help of tools and techniques and suggestions and observations to shareholders whether to buy/sell or hold shares. Study level/applicability This case can be used for MBA (Finance) students on equity research and valuation. Students are introduced to the fundamental procedures of equity research and analysis – evaluating sector desirability, financial modeling, equity valuation methods. To enhance research skills, students are required to acquire basic knowledge on macro and micro economic indicators. This case helps students to analyze non financial and financial aspects of a company and observe quantitative and qualitative aspects of decisions and decide whether to invest or not and give suggestions to sell, buy or hold stocks. Case overview Mr Rahul, a consultant in Karvey brokerage house was about to leave the office on the evening of March 24, 2015 when the phone rang. It was Mr Srirag, one of his clients and close friends who was passionate about investing in shares. Mr Rahul with his two decades of experience in monitoring and advising various investment plans has been continuously advising Srirag on different investments in shares. Srirag said “Rahul! You know that I bought many shares in Jet Airways. While studying the annual reports of Jet Airways 2014-2015 about its business profits and losses, I came across a January to March, 2013 business quarter analysis report that wrote about Jet Airways facing a net loss of 4.95 billion rupees due to over debt burden and interest costs. It also stated that the company sold a 24 per cent stake in 2013 to Etihad for 332$ million which is an Abu Dhabi based airline. The news said that the deal would help the company overcome financial challenges, raise cash, cut costs and gain access to the global flight network. I am worried about whether this deal would allow the company to continue its operations from India or not. I am also concerned about the downfall of Kingfisher, a major setback in the aviation industry in India that owes 8,000 crores to its employees, banks, airports, oil companies. I am worried that either my investment in Jet Airways might bring huge losses or the partnership with Etihad airways would result in the reduction of costs and due to joint sales efforts, sharing resources and network integration thereby leading to a valuable share price. Since your guidance has helped in many issues, I would like to know the present condition and future prospectus prevailing in Jet Airways”. With a lot of ambiguity in his mind, he asked Rahul to recommend if he should hold or sell the shares in Jet Airways. Expected learning outcomes The case is expected to help the students understand and analyze the following points: the overall performance of the company and industry, how fundamental and technical analysis is applied to reach investment decisions, the areas where Jet Airways occupies the top position compared to peer group (Competitor analysis), the company’s financial position and valuation with the help of tools and techniques and suggestions and observations to shareholders on whether to buy/sell or hold shares. Supplementary materials The link to the following videos to be sent to participants in advance to help them prepare for the class. www.youtube.com/watch?v=_3XJXTmILyk, Equity Research Presentation: Coca-Cola, www.youtube.com/watch?v=n5pEK_2uItg Write Equity Research Report, format, process, www.youtube.com/watch?v=mMLJccgiSTk Equity Valuation and Analysis-Part I. Subject code CSS 1: Accounting and Finance.
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Eliason, Chad M., Jenna M. McCullough, Shannon J. Hackett, and Michael J. Andersen. "Complex plumages spur rapid color diversification in kingfishers (Aves: Alcedinidae)." eLife 12 (April 21, 2023). http://dx.doi.org/10.7554/elife.83426.
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Colorful signals in nature provide some of the most stunning examples of rapid phenotypic evolution. Yet, studying color pattern evolution has been historically difficult owing to differences in perceptual ability of humans and analytical challenges with studying how complex color patterns evolve. Island systems provide a natural laboratory for testing hypotheses about the direction and magnitude of phenotypic change. A recent study found that plumage colors of island species are darker and less complex than continental species. Whether such shifts in plumage complexity are associated with increased rates of color evolution remains unknown. Here, we use geometric morphometric techniques to test the hypothesis that plumage complexity and insularity interact to influence color diversity in a species-rich clade of colorful birds—kingfishers (Aves: Alcedinidae). In particular, we test three predictions: (1) plumage complexity enhances interspecific rates of color evolution, (2) plumage complexity is lower on islands, and (3) rates of plumage color evolution are higher on islands. Our results show that more complex plumages result in more diverse colors among species and that island species have higher rates of color evolution. Importantly, we found that island species did not have more complex plumages than their continental relatives. Thus, complexity may be a key innovation that facilitates evolutionary response of individual color patches to distinct selection pressures on islands, rather than being a direct target of selection itself. This study demonstrates how a truly multivariate treatment of color data can reveal evolutionary patterns that might otherwise go unnoticed.
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Thacker, Tom, Philip J. Seddon, Yolanda van Heezik, and Gerald McCormack. "A myna problem: alien species no obstacle to recovery for the Mangaia kingfisher." Oryx, November 19, 2020, 1–6. http://dx.doi.org/10.1017/s0030605320000071.
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Abstract Endemic island species are of conservation interest as unique taxa, often with restricted populations, but many are data poor. The Mangaia kingfisher Todiramphus ruficollaris, known locally as the tanga‘eo, is endemic to the island of Mangaia in the Cook Islands, and categorized as Vulnerable on the IUCN Red List. The population size has not been estimated since 1996, despite concerns over habitat loss and competition with an invasive species, the common myna Acridotheres tristis. We provide new population estimates for both the tanga‘eo and the common myna, using the same methodology as previous estimates. During December 2018–February 2019 we surveyed with distance sampling along 73 line transects walked across the six habitat types on Mangaia. We estimate there are 4,106 tanga‘eo on Mangaia (95% CI 3,191–5,283), a dramatic 7–8 fold increase compared to the previous estimate of 393–764. We estimate there are 13,350 common myna (95% CI 10,998–16,206), a slight increase, although densities in the two most favoured habitats for myna have declined. There is no evidence that the common myna poses a threat to the viability of the tanga‘eo population, as the latter has increased despite a much larger population of common myna. Presumed declines in the tanga‘eo population in the past were probably a result of habitat loss as a result of the cultivation of pineapples Ananas comosus for export, an industry that collapsed in the 1980s. We recommend a review of the IUCN Red List status of the tanga‘eo.
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MÜLAZIMOĞLU, Melis. "ECO-POETICS OF CHARLES OLSON: AN ECOCRITICAL STUDY ON “THE KINGFISHERS”." HOMEROS, April 30, 2021. http://dx.doi.org/10.33390/homeros.4.2.02.
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This paper interprets Charles Olson’s poem, “The Kingfishers” (1949) through ecocritical lenses. Although not counted necessarily as an environmentalist, ecopoet or nature writer, many of Olson’s works can be scrutinized within the frame of Ecocriticism, which as a literary theory and critical approach emerging in the Western academia by the 1990s, is mainly defined as the “study of the relationship between the literature and the physical environment” (Glotfelty and Fromm, eds., 1996: xviii). Progressing towards an eco-centric universe rather than anthropocentric in alternative ways that will help continue interaction among species and lead to organic sustainability, Ecocriticism both as a method and practice aims to revitalize the literary conception and representation of human and non-human universes. On the other hand, what triggers Olson’s ecopoetics has been parallel to some of the basic tenets of Ecocriticism: Olson’s works demonstrate his critique of the Western logocentric thinking that undermines, exploits and silences nature as the non-human other. Moreover, his acknowledgement of the primordial cultures of Americas as well as his preoccupation with the fauna and flora of his hometown, Gloucester-Massachusetts serves as an example of “bio-regionalism” in connection with the larger spectrum. Thus, this paper handles the poem “The Kingfishers” with an ecocritical approach in trying to exemplify the ecological awareness in Charles Olson. The entanglement between verse and universe interpreted within Ecocritical discourse will be discussed in terms of subtitles such as “poetry as dwelling,” “bio-regionalism” and “the concept of interconnectedness and theory of rhizome” all of which render service to the ecocritical emphasis on the “sustainability of literature.”
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Gupta, Priyanka, Adarsh Anand, Yoshinobu Tamura, and Mangey Ram. "Risk-based reliability assessment and testing stop time based software system modeling." International Journal of Quality & Reliability Management, July 4, 2023. http://dx.doi.org/10.1108/ijqrm-06-2022-0186.
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PurposeThe ideology of this article is to study the performance concerns of SDN Controllers, with the help of developed SRGM and thereby obtain its optimal testing duration. The effect of undetected uncertainty in the parameter values have also been catered in the proposal.Design/methodology/approachThese uncertainties in the parameter values are studied as the risk of not meeting desired set of requirements, whose removal causes additional cost. Considering these two constructs as attributes of MAUT, the controller's optimal testing duration is obtained.FindingsThe article focuses towards obtaining the optimal duration for which the SDN Controllers must be tested. It was observed that the inculcation of risk-attribute has provided the higher utility value as compared to any other existing scenarios.Originality/valuePlenty of SRGM have been proposed in the literature which talks about the testing stop time determination problems. But, none of them have considered the impact of risk of not meeting the requirements (reliability) along with cost to obtain its testing stop time. Further, validation of the proposed model in presented with the help of two releases versions of SDN controller platform, ONOS, entitled as “Kingfisher” and “Loon” and has acquired promising results.
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Lim Kam Sian, Terry C. C., Gabriel Goncalves, Joel R. Steele, Tima Shamekhi, Liesl Bramberger, Dongbin Jin, Mohammad Shahbazy, et al. "SAPrIm, a semi-automated protocol for mid-throughput immunopeptidomics." Frontiers in Immunology 14 (June 2, 2023). http://dx.doi.org/10.3389/fimmu.2023.1107576.
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Human leukocyte antigen (HLA) molecules play a crucial role in directing adaptive immune responses based on the nature of their peptide ligands, collectively coined the immunopeptidome. As such, the study of HLA molecules has been of major interest in the development of cancer immunotherapies such as vaccines and T-cell therapies. Hence, a comprehensive understanding and profiling of the immunopeptidome is required to foster the growth of these personalised solutions. We herein describe SAPrIm, an Immunopeptidomics tool for the Mid-Throughput era. This is a semi-automated workflow involving the KingFisher platform to isolate immunopeptidomes using anti-HLA antibodies coupled to a hyper-porous magnetic protein A microbead, a variable window data independent acquisition (DIA) method and the ability to run up to 12 samples in parallel. Using this workflow, we were able to concordantly identify and quantify ~400 - 13000 unique peptides from 5e5 - 5e7 cells, respectively. Overall, we propose that the application of this workflow will be crucial for the future of immunopeptidome profiling, especially for mid-size cohorts and comparative immunopeptidomics studies.
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Clarke, Thomas M., Sasha K. Whitmarsh, Jenna L. Hounslow, Adrian C. Gleiss, Nicholas L. Payne, and Charlie Huveneers. "Using tri-axial accelerometer loggers to identify spawning behaviours of large pelagic fish." Movement Ecology 9, no. 1 (May 24, 2021). http://dx.doi.org/10.1186/s40462-021-00248-8.
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Abstract Background Tri-axial accelerometers have been used to remotely describe and identify in situ behaviours of a range of animals without requiring direct observations. Datasets collected from these accelerometers (i.e. acceleration, body position) are often large, requiring development of semi-automated analyses to classify behaviours. Marine fishes exhibit many “burst” behaviours with high amplitude accelerations that are difficult to interpret and differentiate. This has constrained the development of accurate automated techniques to identify different “burst” behaviours occurring naturally, where direct observations are not possible. Methods We trained a random forest machine learning algorithm based on 624 h of accelerometer data from six captive yellowtail kingfish during spawning periods. We identified five distinct behaviours (swim, feed, chafe, escape, and courtship), which were used to train the model based on 58 predictive variables. Results Overall accuracy of the model was 94%. Classification of each behavioural class was variable; F1 scores ranged from 0.48 (chafe) – 0.99 (swim). The model was subsequently applied to accelerometer data from eight free-ranging kingfish, and all behaviour classes described from captive fish were predicted by the model to occur, including 19 events of courtship behaviours ranging from 3 s to 108 min in duration. Conclusion Our findings provide a novel approach of applying a supervised machine learning model on free-ranging animals, which has previously been predominantly constrained to direct observations of behaviours and not predicted from an unseen dataset. Additionally, our findings identify typically ambiguous spawning and courtship behaviours of a large pelagic fish as they naturally occur.
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Kalsie, Anjala, and Ashima Arora. "Use of Altman’s Z score and Merton Model by Banks to Predict Bankruptcy in Indian Corporates." IMS Manthan (The Journal of Innovations) 11, no. 01 (June 28, 2016). http://dx.doi.org/10.18701/imsmanthan.v11i01.6873.
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There has been a recent increase in the financial distress in assets of the banking industry. Constant defaults by corporate houses have led banks reach gross NPA levels of 5%.One major reason for this rise can be improper risk assessment by banks while giving out loans and improper monitoring of their portfolio companies. And further Indian banks do not use the most robust risk assessment tools and fail in taking early warning action. The paper tries to use two popular models, namely the Altman Zscore model and the Merton Model to predict financial distress in companies which have been the top defaulters in the recent past.The paper tried to determine whether such models are effective in predicting financial distress and how much before the occurrence of the actual event. Financial ratios and other quantitative data from March 2009- March 2013 forms the sample for the study for Kingfisher Airlines, MoserBaer, Gammon India, Educomp Solutions and Deccan Chronicles. The study found Merton model to be a better indicator of financial distress (of companies) than Altman Z score model. However, none of the models were able to judge the possibility of default at the time of issuance of loan indicating at its limitation. Nevertheless, these models which essentially are data driven for assessing the credit risk must widely be used by banks more often, replacing the existing reliance on simple ratios and intuition method.
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Kulshrestha, Reeti, Arunaditya Sahay, and Subhanjan Sengupta. "IndiGo: Cruising in Market but Crashing Within." Asian Journal of Management Cases, April 22, 2021, 097282012199495. http://dx.doi.org/10.1177/0972820121994959.
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M. Damodaran,1 chairman IndiGo, has a lot on his plate, and the servings are not over yet. What happened on 26 April 2019, was just a precursor to what was eventually to follow. Aditya Ghosh,2 the longstanding director of InterGlobe3 for 10 years, resigned from his position, making way for Greg Taylor4 as president and chief executive officer (CEO). Rahul Bhatia5 became the interim CEO. This, ironically, happened when the airline had bagged in traffic rights to as many as 15 countries, including France, UK and Germany. The most ill-timed dispute between the two co-founders of IndiGo, Rakesh Gangwal6 and Rahul Bhatia, which had been brewing for about a year, came out in the open on 16 July 2019, at the most inopportune moment. Rakesh Gangwal alleged violations of corporate governance rule at IndiGo7 and requested the Securities and Exchange Board of India8 (SEBI) to intervene. The feud between the founders of InterGlobe Aviation Ltd. opened a can of worms, although Gangwal was not inclined to sell or raise his stakes. Analysts wondered about the timing of the complaints to SEBI: Why now? Will IndiGo be able to come out of this predicament or follow Kingfisher and Jet Airways’ footsteps?11 Will it be yet another episode of shallow vested interests? Will this lead to the downfall of IndiGo,12 or will it survive the turbulence and keep flying like a phoenix?
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Kim, Hankyu, Yongwon Mo, Chang-Yong Choi, Brenda C. McComb, and Matthew G. Betts. "Declines in Common and Migratory Breeding Landbird Species in South Korea Over the Past Two Decades." Frontiers in Ecology and Evolution 9 (March 29, 2021). http://dx.doi.org/10.3389/fevo.2021.627765.
Full textAbstract:
Population declines in terrestrial bird species have been reported across temperate regions in the world and are attributed to habitat loss, climate change, or other direct mortality sources. North American and European studies indicate that long-distance migrants, common species, and species associated with grasslands and agricultural lands are declining at the greatest rates. However, data from East Asia on avian population trends and associated drivers are extremely sparse. We modeled changes in occupancy of 52 common breeding landbird species in South Korea between 1997–2005 and 2013–2019. Thirty-eight percent of the species showed evidence of declines, and seven of these were declining severely (46–95%). Occupancy of Black-capped Kingfisher (Halcyon pileata) populations have dropped the most precipitously over the study period. Among declining species, long-distance migrants (9/20) and common species (14/20) showed more rapid declines than other groups. Declines of five species were associated with climate change, and two species appeared to be affected by land-cover change. However, causes of change in occupancy of other species (46/52) remains cryptic. Based on our results, we suggest an immediate re-evaluation of species’ conservation status and legal protection levels for seven severely declining species in South Korea, and a dedicated survey design and analysis effort for the continued monitoring landbird populations. Because many species exhibiting declines migrate from beyond national boundaries, international collaborations will be required to better quantify population trends across the full annual cycle, and to understand mechanisms for these declines.
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Yong, Ding Li, Wieland Heim, Sayam U. Chowdhury, Chang-Yong Choi, Pavel Ktitorov, Olga Kulikova, Alexander Kondratyev, et al. "The State of Migratory Landbirds in the East Asian Flyway: Distributions, Threats, and Conservation Needs." Frontiers in Ecology and Evolution 9 (April 13, 2021). http://dx.doi.org/10.3389/fevo.2021.613172.
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With nearly 400 migratory landbird species, the East Asian Flyway is the most diverse of the world’s flyways. This diversity is a consequence of the varied ecological niches provided by biomes ranging from broadleaf forests to arctic tundra and accentuated by complex biogeographic processes. The distribution and migration ecology of East Asian landbirds is still inadequately known, but a recent explosion in the number of studies tracking the migration of raptors, cuckoos, kingfishers and passerines has greatly increased our knowledge about the stopover and wintering ecology of many species, and the migratory routes that link northeast Eurasia and the Asian tropics. Yet the East Asian Flyway also supports the highest number of threatened species among flyways. Strong declines have been detected in buntings (Emberizidae) and other long-distance migrants. While the conservation of migratory landbirds in this region has largely focused on unsustainable hunting, there are other threats, such as habitat loss and increased agro-chemical use driven directly by land cover change and climate-related processes. Important knowledge gaps to be addressed include (1) threats affecting species in different parts of their annual cycle, (2) range-wide population trends, (3) ecological requirements and habitat use during the non-breeding season, and (4) the conservation status of critical wintering sites (including understudied farming landscapes, such as rice fields) and migration bottlenecks along the flyway.
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Kerwath, Sven, Rouvay Roodt-Wilding, Toufiek Samaai, Henning Winker, Wendy West, Sheroma Surajnarayan, Belinda Swart, et al. "Shallow seamounts represent speciation islands for circumglobal yellowtail Seriola lalandi." Scientific Reports 11, no. 1 (February 11, 2021). http://dx.doi.org/10.1038/s41598-021-82501-z.
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AbstractPhenotypic plasticity in life-history traits in response to heterogeneous environments has been observed in a number of fishes. Conversely, genetic structure has recently been detected in even the most wide ranging pelagic teleost fish and shark species with massive dispersal potential, putting into question previous expectations of panmixia. Shallow oceanic seamounts are known aggregation sites for pelagic species, but their role in genetic structuring of widely distributed species remains poorly understood. The yellowtail kingfish (Seriola lalandi), a commercially valuable, circumglobal, epipelagic fish species occurs in two genetically distinct Southern Hemisphere populations (South Pacific and southern Africa) with low levels of gene-flow between the regions. Two shallow oceanic seamounts exist in the ocean basins around southern Africa; Vema and Walters Shoal in the Atlantic and Indian oceans, respectively. We analysed rare samples from these remote locations and from the South African continental shelf to assess genetic structure and population connectivity inS. lalandiand investigated life-history traits by comparing diet, age, growth and maturation among the three sites. The results suggest that yellowtail from South Africa and the two seamounts are genetically and phenotypically distinct. Rather than mere feeding oases, we postulate that these seamounts represent islands of breeding populations with site-specific adaptations.
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Feisst, Debbie. "The Whole Truth by K. Pearson." Deakin Review of Children's Literature 2, no. 2 (October 9, 2012). http://dx.doi.org/10.20361/g2sw2h.
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Pearson, Kit. The Whole Truth. Toronto: Harper Collins, 2011. Print. Victoria, B.C.-based children’s author (and one-time children’s librarian!), Kit Pearson’s tale of family intrigue will have you hooked from the first line, ‘After it happened they were sent away.” A Governor General Award-winning author for Awake and Dreaming, Pearson won the 2012 Canadian Library Association’s Book of the Year for Children Award for The Whole Truth, which delightfully has been followed with a sequel, And Nothing But the Truth. The year is 1932, and, like many areas, Winnipeg is gripped by the Depression. Following a tragic incident which resulted in the drowning death of their father, nine-year-old Polly and her older sister Maud must make a long journey, first by train and then by ferry boat, to Kingfisher Island, a small island near Victoria, to live with their maternal grandmother whom the girls do not remember. Maud will attend boarding school in Victoria while Polly will go to the small island school. Before Maud leaves, however, she makes Polly swear a strict promise to keep a secret regarding their father. Polly’s daily struggles with her grief and loneliness are juxtaposed with her desire to be happy. Maud deals with her sadness by pouring her energies into her new friendships and all that comes with attending a boarding school: uniforms to buy, sporting events, and school plays. The girls’ well-respected and loving grandmother, along with their interesting extended family, provide a safe home, and Polly’s school offers new opportunities for friendship and adventure. But Polly is just a young girl, and keeping a secret weighs heavily on her. She copes by writing touching yet so very sad letters to her deceased father. As the shocking secret is revealed, Polly and Maud’s newfound happiness is threatened and the bounds of family loyalty are tested. I eagerly await the sequel to this novel and the next chapter in the lives of Polly and Maud. Highly recommended: 4 out of 4 stars Reviewer: Debbie Feisst Debbie is a Public Services Librarian at the H.T. Coutts Education Library at the University of Alberta. When not renovating, she enjoys travel, fitness and young adult fiction.
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Liu, Angela, Igor Pirozzi, Basseer M. Codabaccus, Frances Stephens, David S. Francis, Jesmond Sammut, and Mark A. Booth. "Effects of dietary choline on liver lipid composition, liver histology and plasma biochemistry of juvenile yellowtail kingfish (Seriola lalandi)." British Journal of Nutrition, September 18, 2020, 1–15. http://dx.doi.org/10.1017/s0007114520003669.
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Abstract Choline plays a crucial role in lipid metabolism for fish, and its deficiency in aquafeed has been linked to compromised health and growth performance. A 56-d experiment was conducted to examine the effects of dietary choline on lipid composition, histology and plasma biochemistry of yellowtail kingfish (Seriola lalandi; YTK; 156 g initial body weight). The dietary choline content ranged from 0·59 to 6·22 g/kg diet. 2-Amino-2-methyl-1-propanol (AMP) (3 g/kg) was added to diets, except for a control diet, to limit de novo choline synthesis. The results showed that the liver lipid content of YTK was similar among diets containing AMP and dominated by NEFA. In contrast, fish fed the control diet had significantly elevated liver TAG. Generally, the SFA, MUFA and PUFA content of liver lipid in fish fed diets containing AMP was not influenced by choline content. The SFA and MUFA content of liver lipid in fish fed the control diet was similar to other diets except for a decrease in PUFA. The linear relationship between lipid digestibility and plasma cholesterol was significant, otherwise most parameters were unaffected. When AMP is present, higher dietary choline reduced the severity of some hepatic lesions. The present study demonstrated that choline deficiency affects some plasma and liver histology parameters in juvenile YTK which might be useful fish health indicators. Importantly, the present study elucidated potential reasons for lower growth in choline-deficient YTK and increased the knowledge on choline metabolism in the fish.