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Journal articles on the topic "Kim Cascone"

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Mora Medina, Paula. "La ubicuidad digital en la contemporaneidad. Una reflexión sobre el arte postdigital." Revista Eviterna, no. 9 (March 21, 2021): 63–78. http://dx.doi.org/10.24310/eviternare.vi9.11157.

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Tomando como punto de partida el proceso de ubicuidad digital contemporáneo y sus consecuencias políticas, sociales y económicas; el presente trabajo propone una definición de “arte postdigital”. Ésta, más que revisar las consecuencias de dicha situación en el campo de la cultura, tiene como objetivo convertirse en espacio de análisis y reflexión ante las consecuencias sociales, políticas y económicas de la presente situación, marcada por el denominado “capitalismo de plataformas”, una propuesta de Nick Srnicek para definir un sistema capitalista fundamentado en el tráfico de datos. Con este objetivo, se expone, en primer lugar, un análisis de la construcción del término a través de figuras como Kim Cascone o Florian Cramer. A continuación, y con el objeto de seguir analizando el uso del término, se presenta un estudio de la inclusión de éste en el campo institucional de las artes, observando cómo la mayoría de las aportaciones se han acogido a las mencionadas figuras y, por tanto, a una noción de “postdigital” que analiza, únicamente, las consecuencias de la ubicuidad digital en la cultura. Por último, y con el fin de conformar nuestra propuesta de “arte postdigital”, se presenta una clasificación de las prácticas artísticas que responden a nuestra propia definición. Esta clasificación responde a tres temáticas: la materialidad de las herramientas digitales, el análisis y uso del error de las tecnologías y, por último, la identidad.
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Zhuang, Yibo, Fei Zhao, Jing Liang, Xu Deng, Yue Zhang, Guixia Ding, Aihua Zhang, Zhanjun Jia, and Songming Huang. "Activation of COX-2/mPGES-1/PGE2 Cascade via NLRP3 Inflammasome Contributes to Albumin-Induced Proximal Tubule Cell Injury." Cellular Physiology and Biochemistry 42, no. 2 (2017): 797–807. http://dx.doi.org/10.1159/000478070.

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Background/Aims: The activation of NOD-like receptor family, pyrin domain containing3 (NLRP3) inflammasome has been shown to be positively correlated with the severity of proteinuria in chronic kidney disease (CKD) patients. Prostaglandin E2 (PGE2), an important inflammatory mediator, is also involved in various kidney injuries. The aim of the present study was to investigate the involvement of NLRP3 inflammasome and PGE2 synthetic pathway in albumin-induced renal tubular injury. Methods: Murine proximal tubular cells (mPTCs) were treated with albumin to induce cell injury. NLRP3 siRNA and specific COX-2 inhibitor NS398 were used to define their roles in mediating albumin-induced mPTC injury or the activation of COX-2/mPGES-1/PGE2 cascade. Results: In mPCTs, inhibition of NLRP3 by a small interfering RNA (siRNA) blocked albumin-induced kidney injury molecule 1 (KIM-1) upregulation, inflammatory response, and cell apoptosis. Albumin markedly activated cyclooxygenase-2 (COX-2)/ microsomal prostaglandin E synthase-1 (mPGES-1)/PGE2 pathway in this cell line, an effect largely abolished by NLRP3 silencing at both mRNA and protein levels. More interestingly, blockade of COX-2 using a specific COX-2 inhibitor NS398 markedly inhibited the upregulation of KIM-1 and inflammatory cytokines, and attenuated cell apoptosis in line with blunted PGE2 release following albumin treatment. Conclusions: The findings suggest that COX-2/mPGES-1/PGE2 axis could be activated by albumin in the proximal tubular cells via a NLRP3 inflammasome-mediated mechanism and could thus contribute to proteinuria-related renal tubular cell injury.
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Poletaev, Gennady, Darya Novoselova, Mikhail D. Starostenkov, Vladimir Tsellermaer, and Viktor Kovalenko. "The Study of Inhibition of Atom-Atom Collisions Cascades by Ni-Al (100) Interphase Boundary." Key Engineering Materials 685 (February 2016): 8–12. http://dx.doi.org/10.4028/www.scientific.net/kem.685.8.

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The interaction of atom-atom collisions cascades with Ni-Al interphase boundary was studied by the method of molecular dynamics. It was shown that the interphase boundary partially absorbs the cascade energy. The degree of energy absorption of the cascade by the interphase boundary increases with the growth of the structural imperfection of the boundary and density of the misfit dislocations, and also with increase of distance between the boundary and the place of the cascade initiation.
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Kupchishin, Anatoliy I., Alexander A. Kupchishin, Natalia A. Voronova, and Viktor M. Lisitsyn. "Cascade Probability Method and its Relationship with Boltzmann Equations." Key Engineering Materials 712 (September 2016): 123–29. http://dx.doi.org/10.4028/www.scientific.net/kem.712.123.

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The paper suggests and develops a cascade probability method (in particular, for flows of plasma particles), the essence of which is to obtain and apply cascade probability functions (CPF) to cases of different particles. CPF is the probability of particles formed at a certain depth h' reaching a certain depth h after i collisions. The work has considered the interaction of particles with matter; yielded the general solution for cascade probability method and a particular solution for the case of a collided particle not changing its direction and the flow rate not depending on time.
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Kupchishin, Anatoliy I., Nataliya A. Voronova, Tatyana A. Shmygaleva, and Alexander A. Kupchishin. "Computer Simulation of Vacancy Clusters Distribution by Depth in Molybdenum Irradiated by Alpha Particles." Key Engineering Materials 781 (September 2018): 3–7. http://dx.doi.org/10.4028/www.scientific.net/kem.781.3.

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Computer simulation of vacancy clusters distribution by depth in molybdenum irradiated by alpha particles within Cascade probability method has been carried out. Cascade probability functions have been calculated taking into account energy losses for alpha particles in molybdenum and depending on number of interactions and depth of particles penetration. Microhardness comparison of calculations and modified experimental data show their satisfactory fit.
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Liu, Chuanyu, Armin Köhl, and Detlef Stammer. "Adjoint-Based Estimation of Eddy-Induced Tracer Mixing Parameters in the Global Ocean." Journal of Physical Oceanography 42, no. 7 (July 1, 2012): 1186–206. http://dx.doi.org/10.1175/jpo-d-11-0162.1.

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Abstract Using the German Estimating the Circulation and Climate of the Ocean (GECCO) synthesis framework, four separate eddy tracer mixing coefficients are adjusted jointly with external forcing fields, such as to reduce a global misfit between the model simulations and ocean observations over a single 10-yr period and weighted by uncertainties. The suite of the adjusted eddy tracer mixing coefficients includes the vertical diffusivity kz, the along-isopycnal surface diffusivity kredi, the isopycnal layer thickness diffusivity kgm, and the along-iso-thickness advection coefficient kgmskew. Large and geographically varying adjustments are found in all four parameters, which all together lead to an additional 10% reduction of the total cost function, as compared to using only surface flux parameters. However, their relative contribution to the cost reduction varies from 1% to 50% among the four coefficients, with the adjusted kgm contributing most. Regionally, the estimated kgm ranges from less than −800 to about 2500 m2 s−1. Largest adjustments in kgm reside in the vicinity of large isopycnal slopes and support a mixing length hypothesis; they also likewise support the hypothesis of a critical layer enhancement and high potential density gradient suppression. In a few occasions, resulting negative net kgm values can be found in the core of main currents, suggesting the potential for an inverse energy cascade transfer there. Large adjustments of kredi and kgmskew are found in the vicinity of isopycnal slopes. The adjustments of kz in the tropical thermoclines suggest deficiencies of the mixed layer parameterization.
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Takahashi, Akiyuki, Kotoko Hirose, Naoki Soneda, and Masanori Kikuchi. "Molecular Dynamics Simulation of Displacement Cascade in α-Zr." Key Engineering Materials 306-308 (March 2006): 923–28. http://dx.doi.org/10.4028/www.scientific.net/kem.306-308.923.

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In this work, the point defect production in α-Zr at 600K by displacement cascade with Primary Knock-on Atom (PKA) energy up to 20KeV has been investigated by molecular dynamics (MD) simulations. Especially, the influence of subcascades formation on the point defect and cluster production in 20keV cascades has been investigated in detail. The subcascade formation was seen in 36 cases of 20keV cascade simulations out of 50 cases. As the damaged areas are divided into many small areas, the maximum size of point defect cluster tends to decrease when the displacement cascade is settled. On the other hand, subcascades formation has not had an influence on the number of Frenkel pairs in final state of the 20keV cascades comparatively.
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Xie, Yu Dong, Yan Jun Liu, and Yong Wang. "Design of Cascade-Smith Hybrid Control Structure for Pressure Control." Key Engineering Materials 419-420 (October 2009): 797–800. http://dx.doi.org/10.4028/www.scientific.net/kem.419-420.797.

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A new hybrid-control structure for the compensation of the volume-delay negative effect in the mixed-gas pressure control is designed. The volume-delay mechanism is discussed. A Smith predictor is used in the primary loop of a cascade control system where the secondary loop has a negligible delay while the primary loop has a significant and variant delay. To deal with the sensitivity of Smith predictor to modeling error, the gas pipe model for the Smith predictor can be updated by measuring the gas pressure and choosing the model characteristics through a lookup table. The results show that the proposed control structure brings together the best merits of a cascade control and the Smith predictor structure, and it can provide a better performance than the traditional cascade control.
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Grempler, Martina. "Ensemblebearbeitungen in der Opera buffa an den Wiener Theatern der 1760er Jahre." Die Musikforschung 65, no. 2 (September 22, 2021): 127–45. http://dx.doi.org/10.52412/mf.2012.h2.150.

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Der Beitrag behandelt an verschiedenen Beispielen (v.a. Niccolò Piccinni "La buona figliuola", "Le contadine bizzarre" sowie "Lo sposo burlato", Antonio Sacchini "La contadina in corte", Giuseppe Scolari "La cascina") die Bearbeitungspraxis am Wiener Burg- und Kärntnertortheater zur Zeit von Kaiserin Maria Theresia. Unter der Direktion des Opernkapellmeisters Florian Leopold Gassmann, der sich auf ein besonders hochklassiges Sängerkollektiv stützen konnte, kam es dort zu einer überdurchschnittlich intensiven Revision nicht nur von Arien, sondern auch von Ensembles. Dabei zeigen sich verschiedene Möglichkeiten des Vorgehens von der Hinzufügung oder Streichung einzelner Stimmen über die Einarbeitung von neuen musikalischen Abschnitten bis hin zur vollständigen Neukomposition eines Finaltextes.
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Voronova, Nataliya A., Anatoliy I. Kupchishin, Alexander A. Kupchishin, Akmaral A. Kuatbayeva, and Tatyana A. Shmygaleva. "Computer Modeling of Depth Distribution of Vacancy Nanoclusters in Ion-Irradiated Materials." Key Engineering Materials 769 (April 2018): 358–63. http://dx.doi.org/10.4028/www.scientific.net/kem.769.358.

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In this work, the calculation algorithms of cascade and probability functions and vacancy nanoclusters concentration were developed and their calculations for various incident particles in silicon and iron were made.
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Dissertations / Theses on the topic "Kim Cascone"

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Kelly, Caleb, and n/a. "Cracked and Broken Media in 20th and 21st Century Music and Sound." University of Canberra. Creative Communication, 2007. http://erl.canberra.edu.au./public/adt-AUC20070601.135617.

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From the mid 20th century into the 21st, artists and musicians manipulated, cracked and broke audio media technologies to produce novel, unique and indeterminate sounds and performances. Artists such as John Cage, Nam June Paik, Milian Kn��k, Christian Marclay, Yasunao Tone, Oval and Otomo Yoshihide pulled apart the technologies of music playback, both the playback devices � phonographs and CD players � and the recorded media � vinyl records and Compact Discs. Based in the sound expansion of the 20th century musical avant garde, this practice connects the interdisciplinary Fluxus movement with late 20th century sound art and experimental electronic music. Cracked and broken media techniques play a significant role in 20th century music and sound, and continue to be productive into the 21st. The primary contribution of this thesis is to provide a novel and detailed historical account of these practices. In addition it considers theoretical approaches to this work. After considering approaches through critiques of recording media, and concepts of noise, this thesis proposes novel theorisations focusing on materiality and the everyday. Ultimately it proposes that these practices can be read as precursors to contemporary new media, as music and sound art cracked open the fixed structures of �old media� technologies for their own creative purposes.
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Foster, Loren Lee. "Characterization of the Airborne Particulates Generated by a Spray Polyurethane Foam Insulation Kit." Scholar Commons, 2014. https://scholarcommons.usf.edu/etd/5420.

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Spray Polyurethane Foam insulation (SPF) kits are currently being marketed and sold to do-it-yourselfers to meet various insulating needs. Like commercial SPF systems, the primary health concern with SPF kits is user overexposure to the isocyanates during product application. The potential health risk associated with SPF applications is driven by several factors including (but not limited to): the toxicity of isocyanates; the potentially high exposure intensity; the quantity of isocyanates used in the process; the enclosed nature of the environment in which the product could be applied; the potentially high exposure duration/frequency; and the limited availability of control measures to reduce agent intensity (e.g., personal protective equipment, dilution ventilation). To better understand the potential hazards associated with the use of SPF kits, the current study was designed to provide an initial characterization of user exposure to airborne particulate during the application process. Specifically, the study would aim to answer the following: * What is the particle size distribution of the aerosol a SPF kit user is exposed to during application? * What is the airborne particle mass concentration a SPF kit user is exposed to during application? To answer these questions, a single commercially available SPF kit was selected for use and a mock residential environment was constructed to support repeated applications of SPF. Size-selective and total dust air sampling were conducted during the applications to determine the particle size distribution and mass concentration of aerosols generated by the selected kit. The particle size distributions developed from the size selective sampling results showed the presence of airborne particulate capable of penetration to the gas exchange regions of the respiratory tract. The average mass median diameter and geometric standard deviation of the particle size distributions were 4.6 µm and 2.7 respectively. The total dust sampling results showed mean airborne concentrations of 10.40 mg/m3. Based on the sampling results the study, personal air monitoring is needed to assess the degree of user exposure to methylene diphenyl diisocyanate (MDI) and to provide information for the selection of exposure control methods.
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Book chapters on the topic "Kim Cascone"

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de Moura Oliveira, P. B., John D. Hedengren, and José Boaventura-Cunha. "Bridging Theory to Practice: Feedforward and Cascade Control with TCLab Arduino Kit." In Lecture Notes in Electrical Engineering, 23–32. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-58653-9_3.

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Brooks, Lisa. "Prologue: Caskoak, the Place of Peace." In Our Beloved Kin. Yale University Press, 2018. http://dx.doi.org/10.12987/yale/9780300196733.003.0002.

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This Prologue opens at Caskoak, or Casco Bay, in Wabanaki territory, as English and French explorers, merchants, and settlers are entering Native space. This brief opening offers a unique view of a seemingly familiar scene, by showing a snapshot of the encounter between the English explorer Christopher Levett and the Wabanaki “Queen” of Casco Bay. This brief chapter introduces Native American women leaders and their relationships to diplomacy, agriculture, and early deeds.
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Brooks, Lisa. "The Northern Front: Beyond Replacement Narratives." In Our Beloved Kin. Yale University Press, 2018. http://dx.doi.org/10.12987/yale/9780300196733.003.0013.

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This book concludes where it opened, on the Northern Front of Wabanaki, where the war, and the negotiation of peace, continued long past the summer of 1676. This chapter focuses on a wave of raids that began on the Wabanaki coast, on the same day that Metacom was killed, as well as the efforts of multiple Penacook and Abenaki leaders to end the conflict through diplomacy. It highlights the complex context behind the “surprisal” at Cocheco, involving New Hampshire trader Richard Waldron, and explores the response of Indigenous leaders on the Kennebec River. The chapter concludes with the little-known treaties of Pemaquid and Casco Bay in 1677 and 1678, exploring a wide map of Indigenous communication, diplomacy, and alliance. This wider map also enables acknowledgment of the role of the colonies of New York and New France in both diplomacy and war.
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Alexander, Earl B., Roger G. Coleman, Todd Keeler-Wolfe, and Susan P. Harrison. "Northern Cascade-Fraser River, Domain 7." In Serpentine Geoecology of Western North America. Oxford University Press, 2007. http://dx.doi.org/10.1093/oso/9780195165081.003.0025.

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The Northern Cascade–Fraser River domain conforms to the Northern Cascade Mountains physiographic province in northwestern Washington and southern British Columbia, the San Juan Islands between the southern tip of Vancouver Island and the Northern Cascade Mountains, and much of the Interior Plateau province of British Columbia. The thread that connects these areas is the north–south Straight Creek–Fraser River fault system that runs through the Northern Cascade Mountains and northward along the Fraser River. The localities of domain 7 are along faults that branch off from this major fault system. The Northern Cascade Mountains are indeed mountainous, and the Interior Plateau of British Columbia is an area of dissected plateaus and scattered mountains. The Fraser River flows northwest in the Rocky Mountain Trench, which separates the North American craton on the northeast from accreted terranes on the southwest; then it turns around the northwest end of the Cariboo Mountains to the Interior Plateau. In the Interior Plateau, the Fraser River flows from Prince George south about 500 km to the Northern Cascade Mountains before turning westward toward the Pacific Coast. The northern part of domain 7 is in that part of the Fraser River basin, including tributaries northwest of Prince George, which is in the Interior Plateau province. Low, hilly terrain dominates the San Juan Islands. All of these areas in domain 7, except the Ingalls complex on southeast margin of the Northern Cascade Mountains, were covered by the Cordilleran ice sheet during the last stage of the Pleistocene glaciation, leaving <15 ka years for soil development on the current ground surfaces. Although alpine glaciers formed in the southeastern margin of the Northern Cascade Mountains, they did not cover all of the soils, allowing some of them longer time for development. Elevations in domain 7 range from sea level on San Juan Islands to mostly in the 600–1500 m range on the Interior Plateau of British Columbia, and up to 4392 m on Mt. Rainier in the Northern Cascade Mountains.
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Taber, Douglass F. "Alkaloid Synthesis: Indolizidine 223AB (Cha), Lepadiformine (Kim), Kainic Acid (Fukuyama), Gephyrotoxin (Smith), Premarineosin A (Reynolds)." In Organic Synthesis. Oxford University Press, 2017. http://dx.doi.org/10.1093/oso/9780190646165.003.0059.

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Jin Kun Cha of Wayne State University prepared (Org. Lett. 2014, 16, 6208) the allene 1 by SN2′ coupling of a cyclopropanol with a propargylic tosylate. Silver-mediated cyclization converted 1 into 2, that was reduced with diimide to the Dendrobates alka­loid indolizidine 223AB 3. Sanghee Kim of Seoul National University observed (Chem. Eur. J. 2014, 20, 17433) high diastereoselectivity in the Ireland–Claisen rearrangement of 4 to 5. The acid 5 was the key intermediate for the synthesis of the tunicate alkaloid lepadiformine 6. Tohru Fukuyama of Nagoya University also used (Eur. J. Org. Chem. 2014, 4823) an ester enolate Claisen rearrangement to set the relative and absolute configuration of 7. Pd-catalyzed cyclization then led to 8, that was carried on to the excitatory amino acid receptor agonist kainic acid 9. Gephyrotoxin 12 was so named because it incorporates structural elements from two different classes of the Dendrobates alkaloids. Martin D. Smith of the University of Oxford envisioned (Angew. Chem. Int. Ed. 2014, 53, 13826) the cascade cyclization of deprotected 10 to give, after reduction, the ketone 11. Zhen Yang of the Peking University Shenzhen Graduate School showed (Chem. Eur. J. 2014, 20, 12881) that the Rh carbene derived from 13 readily cyclized to an imine. The facial selectivity of the addition of the Grignard reagent 14 to that imine depended on the temperature of the reaction. At room temperature, 15 was formed. At low temperature, the other diastereomer predominated. Ring-closing metathesis was used for the elaboration of 15 to the Stemona alkaloid tuberostemospiroline 16. Kevin A. Reynolds of Portland State University prepared (J. Org. Chem. 2014, 79, 11674) 19 by condensation of the pyrrole 17 with the aldehyde 18. The biosyn­thetic enzyme, that they had overexpressed, oxidized 19 to the antimalarial alkaloid permarineosin A 20.
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Graham, Alan. "Late Cretaceous through Early Eocene North American Vegetational History: 70-50 Ma." In Late Cretaceous and Cenozoic History of North American Vegetation (North of Mexico). Oxford University Press, 1999. http://dx.doi.org/10.1093/oso/9780195113426.003.0008.

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At the end of the Cretaceous the Appalachian Mountains had undergone 180 m.y. of erosion since their principal uplift in the Middle Pennsylvanian through the Late Permian (300-250 Ma), but they were higher and more rugged than at present and provided a somewhat more diverse vertically zoned array of habitats. In contrast, the Rocky Mountains were only ~1 km above sea level at 65 Ma; the Coast Ranges, Sierra Nevada, and Cascade Mountains would not attain substantial heights until late in the Tertiary. Computer models in the NM mode, which simulate conditions in western North America in the Late Cretaceous, show a nearly continuous westerly jet stream with relatively small amplitude between the troughs (lowpressure systems) and ridges (high-pressure systems). The present north-south seasonal meandering of the jet stream was also less. Thus, in the models precipitation and temperatures were more uniform throughout the year and there was less regional differentiation in climate. CO2 concentrations during the Cretaceous are estimated to have been 4-8 times to 10-12 times higher than at present. With a 2-5°C warming for each doubling of CO2, this provides part of the explanation for the higher MAT documented for the Late Cretaceous and Early Tertiary. High CO2 concentrations near the end of the Cretaceous may have been a holdover from earlier intense volcanism in the South Pacific that began to subside at ~ 100 Ma. The term epeirogeny refers to vertical motions of the Earth’s crust, and these movements affect the ocean floor, as well as the continents. There was a 50% increase in the production of ocean crust in the Middle Cretaceous compared to earlier times, as represented by the early Aptian Ontong Java Plateau, the Earth’s largest oceanic plateau, now submerged over 2 km off the Solomon Islands. A sense of the magnitude of this structure can be gained by comparing its volume with that of the surface-exposed Deccan Traps of India (66 Ma). The latter are ~1 km thick and have a volume of 1.5 x 106 km3. The Ontong Java Plateau is ~36 km thick and has a volume of 50 x 106 km3.
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Alexander, Earl B., Roger G. Coleman, Todd Keeler-Wolfe, and Susan P. Harrison. "Klamath Mountains, Domain 5." In Serpentine Geoecology of Western North America. Oxford University Press, 2007. http://dx.doi.org/10.1093/oso/9780195165081.003.0023.

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Domain 5 has mountains on the west aligned in broad arcs; in the eastern Klamath Mountains this arcuate pattern is not as evident. The outer arc curves from the triple junction of the California Coast Ranges, Great Valley, and Klamath Mountains in western Tehama County around through Del Norte County, where it is within a few kilometers of the Pacific Ocean, to Douglas County in Oregon. This western edge of the Klamath Mountains marks a boundary with the California Coast Ranges. The eastern edge of the Klamath Mountains forms a boundary with the Cascade Mountains. The southern, or southeast, boundary is where sediments of the Great Valley lap over the Klamath Mountains in Shasta and western Tehama counties. Serpentine rocks are more extensive in the Klamath Mountains than in any other domain or physiographic province in North America. Through the middle of the Tertiary, the Klamath Mountains were eroded to a nearly level plain called the Klamath peneplain (Diller 1902). According to Diller, the Klamath peneplain and the submerged coastal area that had been accumulating sediments were uplifted slightly during the Miocene, and erosion reduced the northern California Coast Ranges to a nearly level plain which he called the Bellsprings peneplain, noting that it is practically continuous with the Klamath peneplain. Subsequently discovered sediments of the Weaverville formation were deposited in a depression, or graben, in the Klamath Mountains during the Oligocene, indicating that uplift of the Klamath peneplain must have begun during the Paleogene, before the Miocene. Uplift was intermittent, allowing time for the erosion of broad valleys in less resistant rocks between episodes of uplift. Concordant summits, or mountains with summits of nearly equal elevation, are the evidence that led Diller (1902) to suspect a former peneplain. Although the mountain summits in any particular area are nearly equal or subequal, there is a general increase in summit elevation from the coast inland to 2.5–2.7 km. The altitude of Mt. Eddy on the eastern edge of the Klamath Mountains is 2751 m and that of Mt.
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Conference papers on the topic "Kim Cascone"

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Li, Ching-Wen, Pao-Hsin Yen, and Gou-Jen Wang. "A Cascade Microfluidic Device for High Quality Mitochondria Extraction." In ASME 2015 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/detc2015-46117.

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Recent researches have demonstrated that cells ingest mitochondrial by endocytosis to repair cell damage. This new mitochondrial therapy approach can be used for curing particular disease of neuropathy related diseases. Hence the obtainment of high quality and healthy mitochondria play an important role in mitochondrial based disease therapy. In this study, we propose a cascade microfluidic device for green extraction of healthy mitochondria. The geometry of the device was designed using the commercially available COMSOL package. Soft lithography process was than conducted to realize the device of PDMS. We used C2-GFP cells to demonstrate the efficiency of the proposed cascade microfluidic device. The total protein assay kit (complex I-V) was conducted to examine the extractive protein and the SDS page (Tom 20) was used for measuring the activity of the extracted mitochondria. Experimental results illustrate that the complex I-V expression of the extracted mitochondria by the proposed device is much higher than that of the extracted mitochondria by conventional kit. Furthermore, the results of the Tom 20 expression also demonstrate that our device is able to extract more amounts of mitochondria than the conventional kit.
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