Academic literature on the topic 'Keywords: HaCaT'

In this study, the effects of black vinegar (BA) and niacinamide on lipopolysaccharide (LPS)-treated human keratinocytes, HaCaT cells, were investigated. First of all, BA and niacinamide have no cytotoxicity in HaCaT cells even at high concentrations. LPS treatment triggers the phosphorylation of p38 mitogen-activated protein kinases (MAPK) and the expression of inflammatory enzymes, iNOS and COX-2. In contrast, BA and niacinamide weakened the expression of LPS-induced COX-2 and iNOS. Based on the results, we concluded that BA and niacinamide have effective anti-inflammatory properties in HaCaT cells. Therefore, BA and niacinamide may be used as new alternative treatments for inflammatory skin diseases. Keywords: Black vinegar, HaCaT cell, Inflammation, Keratinocytes, Liposaccharides, Niacinamide

Purpose: To determine the effect of miR-485-5p on keratinocyte proliferation and migration.Methods: Human primary keratinocytes (HaCaT cells) were treated with different concentrations of transforming growth factor-β1 (TGF)-β1. miR-485-5p expression levels were determined using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). MTT (3-[4,5-dimethylthiazol-2- yl]-2,5 diphenyl tetrazolium bromide) and wound healing assays were performed to investigate the regulatory effects of miR-485-5p on cell viability and migration of HaCaT cells. Downstream target gene expression of miR-485-5p was determined using a luciferase activity assay.Results: In HaCaT cells, miR-485-5p was time- and dose-dependently downregulated by TGF-β1 treatment (p < 0.05). Forced expression of miR-485-5p decreased cell viability and migration of HaCaT cells (p < 0.05). Knockdown of miR-485-5p enhanced HaCaT cell viability and migration. Integrin subunit alpha-5 (ITGA5) was predicted and verified to be a downstream target of miR-485-5p in HaCaT cells. Overexpression of ITGA5 attenuated the miR-485-5p-induced decrease of HaCaT cell viability and migration (p < 0.05).Conclusion: MiR-485-5p reduces cell proliferation and migration of keratinocytes through the regulation of ITGA5. This mechanism provides a potential therapeutic strategy for skin wound healing. Keywords: ITGA5, Keratinocyte, Cell migration, MiR-485-5p, Cell proliferation, Wound healing

Ultraviolet (UV) irradiation generates reactive oxygen species (ROS) in cells, which induces sunburn cell formation, melanoma, photoaging, and skin cancer. This study examines the anti-photodamage effects of kudzu root vinegar and adenosine in UVB-exposed human keratinocytes (HaCaT cells). UVB significantly decreased HaCaT cell viability, whereas kudzu root vinegar and adenosine did not exhibit cytotoxic effects and increased the viability of HaCaT cells. To investigate the protective effects of kudzu root vinegar and adenosine on UVB-induced oxidative stress in HaCaT cells, ROS, matrix metalloproteinases (MMPs), and mitogen-activated protein kinase (MAPK) were analyzed. UVB-induced treatment reduced the activity of antioxidant enzymes; however, kudzu root vinegar and adenosine increased their activity. These results indicated that kudzu root vinegar and adenosine exert cytoprotective activity against UVB-induced oxidative stress in HaCaT cells. Moreover, they suppressed the UVB-induced downregulation of MMPs and inhibited the phosphorylation of MAPK induced by UVB-irradiation. Therefore, kudzu root vinegar and adenosine offer anti-oxidative effects, via lowering ROS production, suppressing JNK activation, and downregulating expression of MMPs. Our findings suggest that kudzu root vinegar and adenosine have potential application in preventing skin damage owing to UVB exposure. Keywords: reactive oxygen species (ROS), HaCaT cell, UVB, skin damage, anti-aging

Background: Pectin is a heterogeneous polysaccharide mainly present in citrus fruits and has different biological activities.Objective: High molecular weight Citrus Pectin and modified citrus pectin (MCP) were tested for their cytotoxic, anti-proliferative, and anti-oxidant activity.Methods: The cytotoxicity of pectin was studied against HaCaT cell line (human keratinocyte cell line) using Trypan blue method and LDH-cytotoxicity assay. Anti-proliferative activity was assayed using a WST-1 proliferation kit. Antioxidant activity was determined using the DPPH scavenging assay.Results: MCP and Pectin both reduced the viability of HaCaT cells in a dose dependent manner; however MCP was found to be more cytotoxic than high molecular weight citrus pectin since it had a lower IC50 (300ug/ul). At non-cytotoxic concentrations, the viability of cells decreased with increase of concentration of MCP as determined by the WST-1. MCP exhibited a higher antioxidant effect than pectin (SC50 at a concentration range between 2 and 4mg/ml).Conclusion: This study suggests that MCP exhibits a stronger cytotoxic and anti-proliferative effect on HaCaT cell line than pectin. The most probable explanation of this observation is the different effect due to the variable molecular weight and exposed side-chains of MCP and high molecular weight citrus pectin. Keywords: Cytotoxic, Anti-proliferative, Pectin, MCP, HaCaT cell line

Earthworm, Eutyphoeus gammiei, homogenate (EGH) was screened for wound healing activity on human keratinocyte cell line, HaCat, by cell proliferation and migration assays. The maximum proliferation and migration of keratinocyte cells were observed at the dose of 25μg/ml. As cell proliferation and migration are key factors for wound healing, the study clearly suggests the potential role of earthworm species Eutyphoeus gammiei on wound healing. Keywords: Eutyphoeus gammiei, Keratinocyte, MTT assay, scratch assay.

Cage amides and imides bearing bicyclo[2.2.1]- and bicyclo[2.2.2]-subunits were synthesized and evaluated both for antimicrobial activity toward five key ESKAPE pathogenic bacteria: one Gram‐positive bacteria methicillin‐resistant Staphylococcus aureus (ATCC 43300), four Gram‐negative bacteria Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 700603), Acinetobacter baumannii (ATCC 19606) and Pseudomonas aeruginosa (ATCC 27853) and for antifungal activity towards pathogenic fungal strains Candida albicans (ATCC 90028) and Cryptococcus neoformans var. Grubii (H99; ATCC 208821). Compound VP-4539 with bicyclo[2.2.2]octene motif demonstrated the highest cytotoxic activity towards C. neoformans, while human keratinocytes of HaCaT line, murine fibroblasts of Balb/c 3T3 line and mitogen-activated lymphocytes of peripheral human blood were found to be tolerant to its action. VP-4539 compound did not intercalate into salmon sperm DNA indicating that its cytotoxicity is not related to intercalation into nucleic acid. Keywords: antifungal, antimicrobial, bicyclo[2.2.1]heptane, bicyclo[2.2.2]octene, cytotoxicity, DNA intercalation, human keratinocytes, lymphocytes, сage compounds

Leishmaniasis is a neglected tropical disease that requires timely and inexpensive treatment. For this purpose, a nanoemulsion with a polyene macrolide antibiotic, or amphotericin B (NE-AmB), was developed. This study quantified the amount of drug permeated and retained in intact and lacerated human skin, simulating cutaneous leishmaniasis (CL) processes. Toxicity in macrophage and keratinocyte cell lines, activity against promastigotes and amastigotes of Leishmania tropica, in vivo irritant activity, and histological evidence was evaluated. Results. The amount of drug retained in intact and damaged skin was 750.18 ± 5.43 and 567.97 ± 8.64 µg/g/cm2, respectively. There was no permeation. No apparent toxic effect was observed in HaCaT cell lines. The IC50 of NE-AmB found for promastigotes and amastigotes was 0.26 ± 0.09 and 0.37 ± 0.05 µg/mL, respectively. NE without AmB did show antiparasitic activity. The formulation showed lower IC50 values on both parasite stages than the AmB solution. There was no skin irritation, and histology showed skin improvement with treatment. We suggest that this NE-AmB may be a candidate for in vivo studies in CL patients. Keywords. Leishmaniasis, Amphotericin B, ex vivo permeation studies, in vitro cytotoxicity, in vitro leishmanicidal activity, Draize test, histology.

An optimized enzymatic extraction using protamexTM was established for polysaccharides from Liparis tesellatus eggs (PLE). Total sugars, sulfated glycosaminoglycans, uronic acids, and hexosamines content of PLE were 43.2%, 9.0%, 23.9%, and 1.73%, respectively. Superoxide radical (SOx*) scavenging activity and 2,2-diphenyl-1-picrylhydrazyl (DPPH*) scavenging activity of PLE was tested to proof its antioxidant properties. Results on SOx* scavenging activity revealed that PLE has antioxidant activity although it’s not surpass the activity of Ascorbic acid as standard. DPPH* scavenging activity showed that PLE has activity increasing at a concentration-dependent manner. However, at 1.25 mg/ml, the anti-DPPH activities of PLE lowered, possibly due to the pro-oxidant action of the sample. PLE also has effects on NO production of RAW 264.7 cells, suggestive of their possible immunostimulating potential in the human body. On human keratinocyte (HaCaT cell line), HT29 and AGS cells lines, PLE was proofed has no toxicity and showed proliferative effects. This effect showed that PLE could have potency as wound healing promoter. An inference can be made that, PLE has indispensable antioxidant potencies as well as cell proliferating activities on specific cell lines which are deemed necessary in the field of medicine and their utilization as functional food supplements. Keywords: Antioxidant, Fish eggs, Immunostimulator, Liparis tessellatus, Polysaccharides

This research aims to find out the budgeting process of the National Amil Zakat Agency (LAZNAS) Nurul Hayat. The research data was obtained through the Nurul Hayat Foundation. The method used is a qualitative approach with a descriptive case study method. Data collection techniques using direct interviews. Interviews were conducted with the Director of Finance, Director of Operations, Director of Empowerment, and Director of ZIS. The analysis technique uses data collection, data reduction, data presentation, and drawing conclusions. The results of this study indicate that LAZNAS Nurul Hayat is an institution that uses a mixed method; the budgeting process includes the stages of preparation, budget ratification, implementation, and evaluation reporting. The budget system used is in accordance with a non-profit legal entity in the form of a foundation as stipulated in Law Number 28 of 2004.Keywords: budgeting process, non-profit, Nurul Hayat

This study aims to analyze the strategy of utilizing productive zakat in the Zakat Institution of Nurul Hayat. Every zakat institution needs an appropriate and efficient strategy in managing zakat funds so that every mustahiq can be transformed into muzakki. This study uses a qualitative approach with case study research methods. The technique of collecting data uses direct interviews, documentation and observation. To analyze the data, this paper uses the SWOT Matrix and then uses QSPM to identify a new strategy. Then to find out the validity of the data, researchers used triangulation techniques by matching the findings of primary data and secondary data. The results obtained that through the SWOT analysis obtained four strategies and through the QSPM analysis the following results are obtained: SO strategy, namely the expansion of the program in collaboration with Dukcapil/Depkop to reach mustahiq who are entitled to power through zakat has a score of 3,735; WO's strategy is to form an R&D division with a score of 6.085; The ST strategy of introducing digital e-commerce technology to Mustahiq has a score of 6.085; The WT strategy of selecting mustahiq has a score of 2.715 Keywords: LAZ, Zakat, SWOT, QSPM

Rhus coriaria L. (R. coriaria), Arctium lappa L. (A. lappa) and Echium amoenum Fisch. (E. amoenum) are medicinal herbs with extensive traditional uses, covering anti-inflammatory skin therapy. The present research aims to investigate the anti-inflammatory effects of different extracts of R. coriaria fruit, A. lappa roots and the E. amoenum flowers in human keratinocytes (HaCaT cells), evaluating extracts prepared using different techniques. Firstly, the herbal extracts were prepared using different methods including cold extraction, maceration, and decoction. The extraction solvents used were water, ethanol, ethanol-water (50:50), acetone, ethyl acetate, and dichloromethane. In the first phase of this study the extracts of E. amoenum, A. lappa and the polar (water (WRC), ethanol-water (EWRC), ethanol (ERC) and macerated ethanol (mERC)) extracts of R. coriaria were tested. The cytotoxicity and IL-8 release using, respectively, MTT and ELISA assays, were performed after 6 hours of TNF-α (10 ng/mL) treatment. Based on these results, the mERC and the EWRC were selected as the active extracts and assessed for NF-κB driven transcription, following identical treatment conditions. A non-toxic extract of A. lappa (EWAL) was also tested for its effect on NF-κB signaling. According to the preliminary data obtained, R. coriaria showed the most consistent and promising effects in inhibition of lL-8 and NF-κB signaling.Therefore, starting from this phase of the research the main focus of the study was on this herb and selectively mERC and the EWRC as the active extracts. The challenged cells by TNF-α, under treatment conditions with mERC and EWRC, were analysed for ICAM-1, VEGF, and MMP-9 releases, as well as NF-κB translocation, by ELISA assays. In addition, both active extracts were investigated for the inflammation induced by UVB (40 mJ/cm2) and were chemically profiled through HPLC-UV-DAD analysis. In the second phase of this research, the lipophilic extracts of R. coriaria (ARC, EARC and DCMRC) were evaluated using the cytotoxicity and IL-8 release assays in TNF-α or PMA-treated cells. Based on these results, active extracts were measured for their effects on NF-κB driven transcription. Among the evaluated herbs, only R. coriaria extracts achieved anti-IL-8 activity. Although all the polar extracts of this plant inhibited the TNF-α-induced IL-8 release, just mERC and EWRC suppressed NF-κB activation, ICAM-1, and MMP-9 secretion. EWRC showed higher inhibition on ICAM-1 and MMP-9 with IC50s of 1.76 ± 0.24 and 1.24 ± 0.33 µg/mL, respectively (mean ± s.d.). On the contrary, mERC significantly decreased VEGF levels whereas EWRC did not show any effect. The HPLC-UV profile of the extracts revealed higher amount of anthocyanins in EWRC in comparison with mERC. mERC, which showed lower amount of anthocyanins than EWRC, blocked both UVB-induced IL-8 release (with IC50 of 7.61 μg/mL) and translocation of NF-κB (at 10 μg/mL), measured by ELISA assay. The R. coriaria acetone (ARC) and ethyl acetate (EARC) extracts significantly inhibited the IL-8 release (at 25 μg/mL), as well as NF-κB driven transcription (at 50 μg/mL), when induced by TNF-α. The dichloromethane extract of this herb failed to show IL-8- inhibitory activity against TNF-α. ARC (with IC50s of 25.77 μg/mL ) and mERC (at 50 μg/mL) showed a significant effect in inhibiting the PMA induced IL-8 release. ARC modulated PMA-induced NF-κB signaling with IC50 of 27.82 μg/mL. Our results suggest the potential positive effect of R. coriaria fruit extracts, mostly mERC, as preventive agent in the treatment of keratinocyte inflammation through their inhibitory effect on the production of skin pro-inflammatory mediators. Our findings on the promising inhibitory effects of R. coriria on NF-κB signaling seem to confirm the traditional use of this herb as a remedy to treat skin inflammatory conditions. Its use as skin anti-inflammatory agent deserves further investigation.

ABSTRACT Background: Anemia is very common in developing countries and is one of the most common complications of serious diseases. Based on the results of screening the Hb levels student at islamic junior high school Miftahul Hayat in 2018, it was found that 37 students had anemia. This study aimed to determine the effectiveness of cookies produced from moringa oleifera leaves on haemoglobin level in students at Islamic Junior High school Miftahul Hayat, Serang, Banten. Subjects and Method: This was a quasi-experimental. A sample of 37 students of MTS Miftahul Hayat, Serang, Banten and randomly allocated into two groups: intervention and control groups. The dependent variable was an increase in hemoglobin value. The independent variable was anemic student. The data were collected from checklist and analyzed using paired t- test. Results: The results of this study were obtained by respondents as many as 33 people because during the research process 4 people did not consume cookies produced from Moringa Oleifera leaves routinely for 14 days because they were sick so they were excluded from the study sample. Hb level before consume consume cookies produced from Moringa Oleifera leaves (Mean = 10.5 gr/ dl; SD= 0.56; p< 0.001). Hb level after consume cookies produced from Moringa Oleifera leaves (Mean= 13 gr/ dl; SD= 0.85). The difference in Hb levels (Mean = 2.5; SD = 0.88; p <0.001), and it was statically significant (p< 0.001) Conclusion: Consume cookies produced from Moringa Oleifera leaves is effective to improve haemoglobin levels in adolescent. Keywords: anemia, teenager, moringa oleifera leaves cookies Correspondence: Dewi Indah Sari. School of Health Polytechnics, Banten. Jl.Dr.Sitanala Kec.Neglasari Kota Tangerang. Email: dewi.indah@poltekkesbanten.ac.id Mobile:+6281906061400 DOI: https://doi.org/10.26911/the7thicph.05.28