Dissertations / Theses on the topic 'KASP markers'

This investigation covers genetics of rust resistance in common wheat and durum wheat. Stripe rust resistance in AUS26615 was conferred by three QTL and these were temporarily named; QYr.sun-1B, QYr.sun-3D and QYr.sun-6B. QYr.sun-1B represented the previously named APR gene Yr29. The other two QTL appear to be new. The detection of QYr.sun-3D in late sown experiment points to its better expression at relatively higher temperature regimes. The all stage resistance gene, YrAW12, carried by AUS26674, was shown to be Yr34 based on its co-segregation with Yr34-linked marker sunKASP_112 and similar seedling expression. AUS26674/Avocet S RIL population was genotyped with Yr18 and Yr29 linked markers, and responses of RILs carrying different combination of these loci were compared. The RILs carrying combination of YrAW12, Yr18 and Yr29 produced adult plant responses equal to the parent AUS266674. Among the two gene combinations, YrAW12 and Yr18 combination, produced adult plant stripe rust score 3, lower than the score 4 exhibited by other two dual combinations). Four RILs that lacked YrAW12, Yr18 and Yr29 displayed stripe rust response scores 5 to 7 indicating the presence of an additional APR locus in AUS266674. Eight leaf rust genes (Lr1, Lr13, Lr16, Lr24, Lr26, Lr27+Lr31, Lr37 and Lr73) and 11 stem rust resistance genes (Sr2, Sr8a, Sr8b, Sr9b, Sr9g, Sr17, Sr23, Sr24, Sr30, Sr31 and Sr38) were postulated singly or in different combinations among a set 85 genotypes. Nine and five lines, respectively, appear to carry uncharacterised leaf rust and stem rust resistance. Adult plant leaf rust responses ranged from 2 to 6, while stem rust scores varied from 2 to 8. Genetic analysis of stem rust resistance in a durum landrace AUS26677 indicated involvement of a single resistance gene, temporarily named SrAW4. SrAW4 was located on chromosome 4B.

This investigation covered characterization of genetically diverse sources of resistance, identification of molecular markers linked with rust resistance genes and their validation across diverse backgrounds. Genetic analysis of leaf rust resistance using durum-specific pathotypes of the leaf rust pathogen revealed the presence of a single gene in Aus26579 and Aus26582. The resistance gene was mapped on chromosome 6BS. Markers sunKASP_60 and sun684 showed close associations with LrAW2. Lr61 was also mapped on chromosome 6BS and haplotype analysis using linked markers suggested that LrAW2 is likely to be Lr61. Aus26582 carried an additional leaf rust resistance gene that was effective against six Australian Pt pathotypes and was mapped on chromosome 3BL and the underlying locus was temporarily named LrAW3. Marker sun786 mapped 1.8 cM distal to LrAW3. As no other seedling leaf rust resistance gene was previously mapped on chromosome 3BL, LrAW3 was formally named Lr79. Markers sun711, sun712, sun725, sunKASP_109 and sun KASP_112 from chromosome 5AL co-segregated with Yr34. Yr48, also located on chromosome 5AL, was proved to be identical to Yr34 on the basis of allelism test, sequence information and haplotype analysis. Various genomic resources were utilized to identify a close genetic association between the marker sun180 and linked rust resistance genes Yr47 and Lr52 in a fine mapping study. The loci order of sun180-0.4 cM-Lr52-0.2 cM-Yr47 was deduced. Markers linked with LrAW2/Lr61 (sunKASP_60), Lr79 (sun786), Yr34/Yr48 (sun712) and linked resistance genes Yr47 and Lr52 (sun180) did not amplify resistance-linked alleles in any of the cultivars lacking these genes and thus demonstrated their robustness in marker-assisted pyramiding with other marker-tagged rust resistance genes to produce triple rust resistant cultivars.

Breeding for rust resistance in wheat relies on detailed understanding and availability of existing genetic diversity for rust resistance among global germplasm and knowledge of pathotypic variation among rust pathogens. This study involved assessment of genetic diversity for stem rust resistance in an international wheat nursery ZWB14, inheritance of stripe rust resistance in a common wheat landrace Aus27881 and molecular mapping of stripe rust resistance in genotype ZIZ13:69 imported from International Centre for Agricultural Research in the Dry Areas (ICARDA). All stage resistance (ASR) genes Sr8a, Sr17, Sr24, Sr30, Sr31 and Sr38 and adult plant resistance (APR) genes Sr2, Sr57 and Sr58 were present either singly or in different combinations in entries of ZWB14. Some entries were observed to carry additional uncharacterised resistance. Tri-genic inheritance of stripe rust resistance in Aus27881 was observed. Genotyping with Yr34 and Yr29 linked markers demonstrated the presence of these genes in Aus27881. Third locus that conditioned APR in Aus27881 appears to be new and needs further characterisation. Genotype ZIZ13:69 was demonstrated to carry ASR gene Yr1 and APR gene Yr29 based on greenhouse tests and marker genotyping, respectively. The targeted genotyping by sequencing (tGBS) assay of a set of RILs lacking Yr29 detected a new quantitative trait locus (QTL) on the long arm of chromosome 7D.

Master of Science
Department of Plant Pathology
John P. Fellers
Allan K. Fritz
Leaf rust is caused by Puccinia triticina and is one of the most widespread diseases of wheat worldwide. Breeding for resistance is one of the most effective methods of control. Lr16 is a leaf rust resistance gene that provides partial resistance at the seedling stage. One objective of this study was to use RNA-seq and in silico subtraction to develop new resistance gene analog (RGA) markers linked to Lr16. RNA was isolated from the susceptible wheat cultivar Thatcher (Tc) and the resistant Thatcher isolines TcLr10, TcLr16, and TcLr21. Using in silico subtraction, Tc isoline ESTs that did not align to the Tc reference were assembled into contigs and analyzed using BLAST. Primers were designed from 137 resistance gene analog sequences not found in Tc. A population of 260 F[subscript]2 lines derived from a cross between the rust-susceptible cultivar Chinese Spring (CS) and a Thatcher isoline containing Lr16 (TcLr16) was developed for mapping these markers. Two RGA markers XRGA266585 and XRGA22128 were identified that mapped 1.1 cM and 23.8 cM from Lr16, respectively. Three SSR markers Xwmc764, Xwmc661, and Xbarc35 mapped between these two RGA markers at distances of 4.1 cM, 10.7 cM, and 16.1 cM from Lr16, respectively. Another objective of this study was to use genotyping-by-sequencing (GBS) to develop single nucleotide polymorphism (SNP) markers closely linked to Lr16. DNA from 22 resistant and 22 susceptible F[subscript]2 plants from a cross between CS and TcLr16 was used for GBS analysis. A total of 39 Kompetitive Allele Specific PCR (KASP) markers were designed from SNPs identified using the UNEAK and Tassel pipelines. The KASP marker XSNP16_TP1456 mapped 0.7 cM proximal to Lr16 in a TcxTcLr16 population consisting of 129 F[subscript]2 plants. These results indicate that both techniques are viable methods to develop new molecular markers. RNA-seq and in silico subtraction were successfully used to develop two new RGA markers linked to Lr16, one of which was more closely linked than known SSR markers. GBS was also successfully used on an F[subscript]2 population to develop a KASP marker that is the most closely linked marker to Lr16 to date.

This investigation covered characterisation of various faba bean rust isolates by identifying a differential set in the host; identification of molecular markers linked with rust resistance genes Uvf-2 in Doza#12034 and Uvf-3 in Ac1655 and their validation across diverse backgrounds; and elucidation of the host-pathogen interactions of Uromyces viciae-fabae with faba bean, field pea (Pisum sativum), lentil (Lens culinaris), chickpea (Cicer arietinum), lupin (Lupinus albus and Lupinus angustifolius) and mungbean (Vigna radiata). The differential pathogenicity in the interaction of Vicia faba × Uromyces viciae-fabae led to the identification of nine faba bean rust pathotypes, characterised by the set of twelve genotypes (regarded as differential), and named 0-10, 0-46, 40-31, 40-55, 24-40, 63-53, 63-49, 55-63 and 63-63. This information will help faba bean breeders to carefully deploy rust resistance gene(s) which can effectively insight resistance against pathotypes of targeted environment. Genetic analysis, using pathotype 24-40, revealed monogenic inheritance of rust resistance in faba bean genotypes Doza#12034 and Ac1655, respectively. After genotyping Fiord/Doza#12034 and Fiord/Ac1655 RILs, two closely linked KASP markers KASP_Vf_0703 and KASP_Ac×F165 were mapped on chromosome III and V with Uvf-2 and Uvf-3, respectively and validated successfully in a set of local/exotic faba bean genotypes. These closely linked markers will allow breeders to implement markers assisted selection for both resistance genes. The histopathology of Australian U. viciae-fabae revealed a host range: both faba bean and field pea were competent hosts showing varietal differences to pathogen responses, with differential expression in resistance; lentil showed complete hypersensitive resistance by expressing cell death; and chickpea, lupin and mungbean appeared as non-hosts.

Migracijos reiškinys yra analizuojamas jau seniai. Iki Lietuvos narystės ES (ir po Lietuvos įstojimo į ES) paskelbta daug darbų migracijos tema. Ypač skaudi valstybei bei aktuali yra aukštąjį išsilavinimą turinčių žmonių emigracija, nes tokiu būdu yra ne tik prarandamos lėšos įdėtos į kvalifikuotos darbo jėgos paruošimą, bet ir prastėja valstybės teikiamų paslaugų kokybė, smunka vidutinis šalies kvalifikacijos lygis, o kartu ir šalies konkurencingumas tarptautinėje rinkoje. Darbo objektas – darbo rinkos politikos įtaka aukštos kvalifikacijos specialistų emigracijai. Pagrindiniai uždaviniai - išryškinti emigracijos esmę bei pasekmes; išskirti teorinius aukštos kvalifikacijos specialistams pritaikytus bei su darbo rinka susijusius emigracijos veiksnius; atskleisti Lietuvoje taikomą migracijos politiką; įvertinti aukštos kvalifikacijos specialistų emigracijos veiksnius Lietuvoje, didelį dėmesį skiriant darbo rinkos politikai; atlikti ketinimų emigruoti dėl darbo rinkos situacijos kiekybinį tyrimą bei sukurti jo rezultatus apibendrinantį modelį. Darbas susideda iš trijų pagrindinių dalių. Pirmojoje darbo dalyje išryškinama teoriniai aukštos kvalifikacijos specialistų migracijos aspektai, antrojoje darbo dalyje nagrinėjama statistiniai su emigracija susiję Lietuvos ir emigracijos tikslo šalių duomenys, trečiojoje darbo dalyje atliekamas empirinis tyrimas, siekiant įvertinti ketinimų emigruoti sąryšį su darbo rinkos situaciją. Pagal tyrimų rezultatus, pateikiamos išvados bei... [toliau žr. visą tekstą]
The subject of the Master work is emigration of highly qualified specialists. Emigration of highly qualified specialists is known for a long period of time, just now the scales of emigration is higher than ever. Economic recession is playing a big part in this, as all the countries are affected by recession at the different level. Due to migration of highly qualified specialists government looses money, which was spend in gaining the qualification, as well country looses tax payers and overall country looses competitiveness in international arena. The object of the study is the influence of labor market policy to the emigration of highly qualified specialists. The main tasks are to highlight the nature and consequences of emigration; to reveal theoretical immigration–related factors; to evaluate Lithuania’s migration policy; focusing on labor market policy to distinguish to high–qualified specialists orientated emigration factors; to perform a quantitative survey and create a model summarizing the results. Work structure: work consists of three main parts: in the first part is made an analysis of theoretical high qualified specialists emigration aspects, in the second part is made a statistical analysis of Lithuania’s and other countries migration related data and in third – an empirical research made to evaluate the influence of labor market factors to the emigration decision. Working volume: 73 pages, 23 tables, 26 figures and 83 references used: 55 Lithuanian and 28... [to full text]

A candidate gene approach was used to identify potential genetic markers associated with wool quality traits including mean fibre diameter (MFD), fibre diameter standard deviation (FDSD), coefficient of variation of fibre diameter (CVD), prickle factor, curvature, yellowness, brightness, staple strength, staple length, yield, greasy fleece weight (GFW) and clean fleece weight (CFW). Inheritance of potential genetic markers was studied in two half-sib Merino families and assessed for association with the wool quality traits. The sire for one of the half-sib families is referred to as MV144-58-00, and wool measurements from its progeny were taken at 12 (n = 131), 24 (n =128) and 36 (n = 37) months of age. The sire for the second half-sib family is referred to as Stoneyhurst, and wool measurements from its progeny (n = 35) were taken at 12 months of age. Genes that code for the keratin intermediate-filament proteins (KRTs) (KRT1.2, KRT2.10) and the keratin intermediate-filament-associated proteins (KAPs) (KAPl.1, KAPl.3, KAP3.2, KAP6.1, KAP 7, KAP8) were targeted for this investigation, along with the beta 3-adrenergic receptor (ADRB3) gene and microsatellites BfMS and OarFCB193. Polymerase chain reaction (PCR) was used to amplify specific DNA fragments from each locus and PCR- single strand conformational polymorphism (PCR-SSCP) analysis was used to detect polymorphism within the half-sib families for all the loci, except for the KAP1.1 gene, where length polymorphism was detected using agarose gel electrophoresis. Only the loci that were heterozygous for the sire (KAP1.1, KAP1.3, KRT1.2, ADRB3, KAP8) and hence were informative, were genotyped in the progeny. The total number of alleles observed at the KAP1.1, KAP1.3, KRT1.2, KAP8 and the ADRB3 loci were four, ten, six, five and six, respectively. Analysis of each of the informative loci revealed allelic associations with various wool traits. In the MV144-58-00 (genotypes KAP1.1 AB; KAP1.3 BD; KRT1.2 AB; ADRB3 CE) half-sib, inheritance of the KAP1.1 A allele was associated with a higher yield at 24 months of age (P = 0.037). This trend also observed at 36 months of age (P = 0.078). At 12 months of age, the KAP1.1 A allele tended to be associated with increased staple length (P = 0.08). At 36 months of age, the inheritance of the KAP1.1 B allele tended towards being associated with whiter wool (P = 0.080). The MV144-58-00 KAP1.3 D allele tended to be associated with increased yield at 24 and 36 months of age (P = 0.091 and 0.059, respectively), and with lower FDSD at 12 months of age (P = 0.055). The sire KAP1.3 B allele was associated with whiter wool colour at 36 months of age (P = 0.045). The inheritance of the MV144-58-00 KR T1.2 B allele was associated with or tended to be associated with a smaller FDSD (P = 0.040), an increase in staple strength (P = 0.025) and an increase in GFW (P = 0.069) at 12 months of age. At 24 months of age, the KR T1.2 B allele tended to be associated with increased yield (P = 0.057). At 36 months of age, the KRTl.2 A allele was associated with whiter wool (P = 0.019) and tended to be associated with increased crimp within the wool fibre (P = 0.089). In the Stoneyhurst (genotypes KAP1.1 BC; KAP1.3 CJ; KRT1.2 DE; ADRB3 CE) half-sib, inheritance of the KAP1.1 B allele was associated with longer staple length (P = 0.018) and a decrease in wool brightness (P = 0.039). In contrast, KAP1.1 C allele was associated with lowest staple length (P = 0.018) and brighter wool colour (P = 0.039). Associations observed with the inheritance of Stoneyhurst KAP 1.1 alleles were similar to the inheritance ofKAPl.3 alleles. Stoneyhurst KAP1.3 J allele was associated with longer staple length (P = 0.017) and a decrease in wool brightness (P = 0.010). In contrast, KAP1.3 C allele was associated with lowest staple length (P = 0.017) and brighter wool colour (P = 0.010). The Stoneyhurst KRT12 D allele was associated with longer staple length and a decrease in wool brightness (P = 0.033). In contrast, KRT1.2 E allele was associated with lowest staple length (P = 0.033) and brighter wool colour (P = 0.022). Sire alleles at the ADRB3 gene locus were associated with variation in staple strength (P = 0.025) for MV144-58-00's progeny, and with variation in yield (P = 0.023) for Stoneyhurst's progeny. The results obtained in this thesis are consistent with KAP1.1, KAP1.3 and KRT1.2 being clustered on one chromosome because both sires in this study passed on two major KAP1.1-KAP1.3-KRT1.2 haplotypes to their progeny, and the associations with wool traits were very similar for all the three loci. The major sire derived KAP1.1 – KAP1.3 - KRT1.2 haplotypes observed within the MV144-58-00 half-sib were: BBA (frequency of 43.4%; n = 43) and ADB (frequency of 44.4%; n = 44). Other minor haplotypes observed were: ADA (frequency of 4.0%; n = 4); BDA (frequency of 2.0%; n = 2); BBB (frequency of 3.0%; n = 3) and BDB (frequency of 3.0%; n = 3). In the Stoneyhurst half-sib, major sire-derived KAP 1.1 - KAP 1.3 - KR Tl.2 haplotypes observed were CCE (frequency of 53.1 %; n = 17) and BJD (frequency of 40.6%; n = 13). The minor haplotype BJE (frequency of 6.3%; n = 2) was also observed. Statistical analyses within the MVI44-58-00 half-sib showed that KAP1.1 AKAP1.3 D - KRT1.2 B haplotype was associated with increased yield (P = 0.023) and tended towards whiter wool colour (P = 0.059), smaller FDSD (P = 0.081) and stronger staple strength (P = 0.092). In the Stoneyhurst half-sib, the KAP1.1 B - KAP1.3 J - KRT1.2 D haplotype was associated with longer staple length (P = 0.010), while the KAP1.1 C - KAP1.3 C - KRT1.2 E haplotype showed a strong trend with increased wool brightness (P = 0.096). Result from this study indicated that the keratin genes on chromosome 11 are recombining relatively frequently at recombination "hotspots". A high rate of recombination among loci that impact on wool traits would make breeding for consistent wool quality very difficult. The results presented in this thesis suggest that genes coding for the KRTs and KAPs have the potential to impact on wool quality. KAP1.1, KAP1.3 and KRT1.2 could potentially be exploited in gene marker-assisted selection programmes within the wool industry to select for animals with increased staple length, 'increased staple strength, higher yield and brighter wool. This study was however limited to two half-sib families, and further investigation is required.

The ability to identify germplasm and select traits accurately is fundamental to successful plant breeding. Pedigrees and molecular markers facilitate these processes; however misleading experimental results can occur when incorrect relationships and/or cultivar names are recorded. Molecular markers can identify these inconsistencies, and with advances in genotyping technology these diagnostics can be done faster and more objectively. This study aimed to develop molecular marker assays and graphical genotyping methodologies for cultivar identification, seed purity assessment and trait selection in oat (Avena sativa L.). KBioscience’s Allele-Specific PCR (KASP™) and genotyping-by-sequencing (GBS) technologies were applied to a set of current Canadian oat cultivars to evaluate their utility for identifying cultivars and detecting intra-cultivar variation. Both KASP™ and GBS detected different extents of heterogeneity among a set of 160 seeds that originated from four seed sources of four cultivars. In both cases, the detected variation did not appear to be limited to a specific cultivar or seed source, reinforcing that all cultivars are heterogeneous. Graphical genotyping localized heterogeneity to specific chromosome regions, thereby distinguishing physical contamination from true genetic heterogeneity and heterozygosity. Pre-existing genotype data for 700 oat cultivars and breeding lines were also used to construct graphical genotypes for pedigree validation and discovery of potential sources for favourable quantitative trait loci (QTL) alleles. This methodology used historical QTLs and anchoring markers to identify 25 putative “high oil” allele carriers. The results from this study will provide diagnostic tools for cultivar identification and pedigree validation, in addition to meaningful information about existing heterogeneity and possible QTL locations in current cultivars.

De lagstadgade bestämmelser som reglerar upphovsrättens övergång återfinns i 3 kap. upphovsrättslagen. Från flera håll har kritiska röster väckts om att dessa bestämmelser behöver överses och moderniseras för att hänga med den kontinuerligt pågående utvecklingen av dels teknik men framför allt den upphovsrättsliga marknaden. I samband med detta har Upphovsrättsutredningen sammanställt ett SOU-förslag som benämnts Avtalad upphovsrätt. Där flerstående förslag till förändringar och tillägg utav de nuvarande bestämmelserna ges. Genom detta SOU-förslag har utredningen noggrant granskat och kommit med förslag till bestämmelser som behöver förändras eller införas. Grundtanken har ansetts vara att undersöka huruvida upphovsmännens ställning som avtalsparter bör stärkas på den upphovsrättsliga marknaden. Denna uppsats kommer att granska dessa förslag, sammanställa dem mot de nuvarande bestämmelserna och framför allt diskutera vilken betydelse dessa förslag har för det nuvarande rättsläget och upphovsmännens ställning.
The stipulations that regulate both the economical and non-profitable aspects of copyright transactions are to be found within the third chapter of the Swedish Copyright Law. Critics have claimed that the current regulations regarding copyright transactions are outdated. It has been pointed out that these regulations need to be updated as regarding to the development of the current technology and the changing copyright market. In accordance with these claims, the board investigating the Swedish Copyright Law - issued by the Swedish government – has presented a motion named Contracted copyright. This motion contains several changes and supplements to the current regulations in the third chapter of the Swedish Copyright Law. The board has through this motion investigated whether and to what extent the current regulations need to be changed in accordance with the current development of the copyright market. The general idea has been to investigate whether the copyright authors require an extended protection on the copyright market. Therefore the purpose of this essay has been to investigate these suggested changes to the current regulations. In order to determine whether they serve their suggested purpose and how they will affect the current legal position.

This doctoral thesis sets out to analyse the development of the unemployed movement in Sweden during the period 1920–34. The study is divided into two parts. The first is empirical and descriptive while the second is interpretive and explanatory, and seeks to examine why this phenomenon developed in the way it did. Mass unemployment in Sweden between the World Wars did not cause the same social tensions as in many other countries. This relative peace endured despite high and consistent unemployment and hard living conditions for the unemployed. These conditions served as sources for tensions present in the unemployed movement, and which some actors sought to take advantage of and even exacerbate. Andréasson argues that a major reason that society did not take a more radical turn in the period was that the reformist labour movement actively moderated these tensions. This was done by the Social Democratic Party (SAP) changing the environment of the unemployed organisations, for example by using local unemployment policy to polish off the rough edges of the national unemployment policy. More important was the crisis politics in the early 1930s that helped narrow the socio-economic gap between those who had and those who did not have a job. The Swedish Trade Union Confederation (LO) neutralised the movement of the unemployed by introducing changes within the unemployed movement itself, involving a variety of strategies. After 1933, the LO and SAP dominated and were able to direct the activities of most of the organisations that existed. Gaining control over the unemployed was as important for the LO and SAP as being able to exert control over other forces that might threaten to weaken their long-term strategies and aims. There was a conviction within the unemployed movement that mass unemployment was largely a consequence of technological developments in production. This argument had roots dating back to the early stages of industrialism in England when Luddites had attacked production machinery. The coalition of organisations of unemployed workers in Sweden during the 1920s and 1930s did not seriously consider engaging in machine-breaking activities. The movement’s criticism of technology did not extend into the Swedish model which envisioned the development of machinery as a way to prevent rising unemployment.
QC 20100628

Syfte och frågeställningar Syftet med uppsatsen är att få en förståelse för vad det är elever som söker till gymnasieskolan kan tänkas möta då de läser recensionstexter. Syftet är således att kartlägga recensionssystemets omfattning och innehåll kvantitativt; vad som tas upp i recensionstexterna, samt att undersöka hur recensionstexterna är konstruerade. Avslutningsvis syftar uppsatsen till att diskutera recensionssystemet i en större samhällskontext - som ett resultat av och en del i vår samtid. Vilka ämnen och teman förekommer i recensionstexter av gymnasieskolor samt hur frekvent är förekomsten?  Hur värderas (positivt, negativt eller neutralt) de olika temana i recensionerna?  På vilket sätt är recensionstexterna konstruerade och hur kan det tolkas? På vilket sätt framträder en skolmarknadsdiskurs i recensionstexterna? Metod De kvantitativa frågorna undersöks genom en innehållsanalys av slumpmässigt utvalda recensioner på gymnasieskolor i Stockholms län. De förekomna orden kvantifieras och delas in i teman, kategorieroch subkategorier. En bedömning görs även utifrån om de förekomna orden lyfts i en positiv, negativ eller neutral kontext. Den kvalitativa delen utgörs av en textanalys inspirerad av diskursanalysen. Av ett antal utvalda recensioner, baserat på dess innehållsliga relevans, analyseras dessa semantiskt med diskursanalytiska verktyg. I diskussions- och analysdelen behandlas resultatet utifrån det teoretiska ramverket och tidigare forskning.  Resultat Av innehållsanalysen framgår att de vanligast förekommande temana var allmänna värdeomdömen om skolan, att kommentera lärare samt skolans upplägg och utbildningens kvalité. Det är även vanligt att sociala aspekter så som atmosfär och gemenskap lyfts fram i recensionerna. Av den kvalitativa delen framgår det att recensenterna uttrycker sig på ett sätt som speglar skolmarknadsdiskursen. I somliga fall framgår tydligt hur recensenten anammat skolmarknadsdiskursen. Slutsats Resultatet indikerar att recensionssystemet riskerar att generera segregerande effekter. Framförallt utifrån den asymmetriska tillgången av information och att skola och identitet knyts allt närmare varandra. Detta förtydligas och ställs på sin spets i hur recensionerna är konstruerade och kan, i vissa fall, tänkas vara svårare att bearbeta då gemene elev tenderar att se recensenter som objektiva (till skillnad från övrig tillgänglig information).
Aim The aim with this study is to explore what kind of information pupils, searching for upper secondary school, might get from reading reviews of schools. The purpose is thus to quantify the scope and content of the review system quantitatively; what is mentioned in the review texts, and to examine how the review are textually constructed. Finally, the paper aims to discuss the review system in a larger social context - as a result of and a part of our time. What topics occur in reviews of upper secondary schools and how frequent is the occurrence? How are the different themes in the reviews valued (positive, negative or neutral)? In what way are the review texts constructed and how can it be interpreted? In what way does a school market discourse appear in the reviews? Method The quantitative questions are examined through a content analysis on randomly selected reviews of upper secondary schools in Stockholm County. The words in question are quantified and divided into themes, categories and subcategories. An assessment is also made based on whether the existing words are lifted in a positive, negative or neutral context. The qualitative part consists of a text analysis inspired by the discourse analysis. From a number of selected reviews, based on its substantive relevance, these are semantically analyzed with discourse analytics tools. In a merged discussion and analysis section, the result is treated on the basis of the theoretical framework and previous research. Results The content analysis shows that the most common themes were general value reviews of the school, commenting on teachers and the school's structure and the quality of the education. It is also common to mention social aspects such as atmosphere and cohesion. From the qualitative part it appears that the reviewers express themselves in a way that reflects the school market discourse. In some cases, it is clear that the reviewer has adopted the school market discourse.  Conclusion The result indicates that the review system risks generating segregating effects. Especially based on the asymmetric availability of information and the fact that school and identity are getting more connected. This is clarified and put on its tip in how the reviews are constructed and, in some cases, may be more difficult to process when the common student tends to see reviewers as objective (in contrast to other available sources of information).

Föreliggande studie undersöker hur tolv oberoende förlag upplever att det är att verka i enägarkoncentrerad bokbransch där de konkurrerar mot vertikalt integrerade mediekoncerner.Studiens fokus ligger på hur respondenterna upplever att den vertikala integrationen påverkarderas möjligheter till exponering på olika typer av plattformar, samt på hur detta formar deoberoende förlagens strategier för konkurrens. De två största förlagsgrupperna i Sverige år2021 är Bonnierförlagen och Norstedts Förlagsgrupp, som båda är del av ett störremediekonglomerat där ägandeskap av både ljudboksappar, fysisk- och internetbokhandelsamt dagspress förekommer. Med utgångspunkt i Pierre Bourdieus fältteori undersöker ochanalyserar rapporten förlagsverksamheternas förutsättningar att verka inom branschen samthur de olika aktörerna agerar och varför. Metoden utgår ifrån kvalitativ innehållsanalys därpersoner i ledande position intervjuas om deras upplevelse av vad det innebär att inte vara endel av ett mediekonglomerat när det kommer till exponering och vilka strategier de har för attkonkurrera mot dem. Resultatet visar att respondenterna ser breda kontaktnät och ekonomisktkapital som viktigt för att ha möjlighet till exponering samt att det kan vara svårt attkonkurrera om synlighet hos vertikalt integrerade distributörer. Respondenterna påverkas avde större mediekoncernernas ställning i branschen i strategierna för att konkurrera mot dem,där de bland annat fyller ut tomrum som de större koncernerna missat. Studiens slutsats är attde oberoende förlagen på olika sätt, beroende på storlek, påverkas av att verka i en branschdär dominerande aktörer är vertikalt integrerade. Det kan sammankopplas med liknandefenomen inom mediebranschen, såsom journalistbranschen.
The study researches the experience of an owner-focused publishing bransch for twelveindependent publishers in which they compete alongside heavily integrated media groups.The study focuses on how the integrated media groups affect the independent publishers intheir implemented strategies to remain competitive. As of the year of 2021, the two largestpublishing groups are Bonnier Publishers and Norstedts Publishing Group. Both are part of alarger media conglomerate that has ownership of audiobook applications, physical andweb-hosted book stores as well as daily press. Starting with Pierre Bourdieu's field theory, thereport investigates and analyses the publishers prerequisites in order to act and remaincompetitive within the industry, as well as how the publishers have to act and the reasons ofwhy. The method is based on qualitative and thorough content analysis where people in aleading position are interviewed about their experience of what it means not to be part of amedia conglomerate when it comes to exhibition and what strategies they have in order toremain competitive. The results show that the respondents see broad contact networks andfinancial capital as the most important pillars in order to have the opportunity for exposureand that it can be difficult to compete for visibility versus heavily integretaded distributors.The respondents are affected by the position the larger media groups hold within the industrybut are prone to utilize strategies that fill gaps that the larger groups have missed. The study'sconclusion is that the independent publishers are affected in different ways, depending ontheir size in an industry where dominant competitors are highly integrated. There are largesimilarities within the media- and journalist industries.

Borage (Borago officinalis) is an annual herb plant for culinary and medicinal uses. Due to a high level of gamma-linolenic acid (GLA) in its seed oil and the health-related benefits of GLA, borage is commercially cultivated. However, a herbicide-resistant variety has not yet been developed for effective weed management in borage farming. Thus, this thesis aimed to create, identify and characterize ethyl methanesulfonate (EMS) induced borage mutants for herbicide imidazolinone resistance. An EMS-mutagenized borage population was generated by using a series of concentrations of EMS to treat M1 seeds. After screening M2 borage plants with the herbicide, tolerant plants were selected, self-pollinated and grown to their maturity. The offsprings were subjected to herbicide screening again to confirm the phenotype, resulting in identification of two genetically stable imidazolinone-resistant lines. Two acetohydroxyacid synthase (AHAS) genes, AHAS1 and AHAS2, involved in the imidazolinone resistance were isolated and sequenced from both mutant (resistant) and wild type (susceptible) borage plants. Comparison of these AHAS sequences revealed that a single nucleotide substitution occurred in the AHAS1 resulting in an amino acid change from serine (S) in the susceptible plant to asparagine (N) in the first resistant line. The similar substitution was later found in the AHAS2 of the second resistant line. A KASP marker was developed for the AHAS1 mutation to differentiate the homozygous susceptible, homozygous and heterozygous resistant borage plants for the breeding purpose. The in vitro assay showed homozygous resistant borage containing the AHAS1 mutation could retain significantly higher AHAS activity than susceptible borage across different imazamox concentrations. The herbicide dose response test showed that the resistant line with the AHAS1 mutation was tolerant to four times the field applied concentration of the “Solo” herbicide.

Title page, abstract and table of contents only. The complete thesis in print form is available from the University of Adelaide Library.
Langdon 7D(7A) and 7D(7B) durum substitution lines were crossed with DBA-Aurora durum wheat to introgress a lutein esterification gene,TaGelp1, from chromosome 7D onto its homoeologues 7A and 7B. Genotyping-by-sequencing based on DNA samples from durum wheat and bread wheat revealed single nucleotide polymorphism (SNPs) among the group-7 chromosomes. Sixteen KASP markers were developed and to be able to differentiate among these chromosomes. Nine 7A-7D markers were used to characterise progeny populations to search for dissociation of molecular markers which may indicate chromosomal recombination. Evidence of possible 7A-7D recombination was found in a small number of progeny (less than 4%). Most of the putative marker dissociations were in the centromeric region but one plant was found to carry only a small distal fragment of 7DS including TaGelp1. The findings suggest crossing normal durum with Langdon 7D(7A) combined with KASP marker assistance can be applied as a method to introgress and assess genes from chromosome 7D onto its homoeologues without resorting to use of wheat with the Ph1 deletion.
Thesis (M.Bio.(PB)) -- University of Adelaide, Masters of Biotechnology (Plant Biotechnology), School of Agriculture, Food and Wine, 2016.

This paper consists of the translation and analysis of Chapters 1-15 of Book V of Historiarum adversus paganos libri VIII by Paulus Orosius. The analysis is based on a comprehensive commentary on linguistic and stylistic aspects of the texts examined, and on a thorough factual commentary. The linguistic commentary concentrates on the composition of the Fifth Book, on the construction of discourse by the means of particles and other discourse markers, as well as on the linguistic features and peculiarities distinctive for the author, his age and the genre of historiography (such as specifically Late Latin syntax, non-Classical vocabulary, rhetorical figures, tropes and other stylistic features). The factual commentary contains a detailed description of the events mentioned in the text and their historical context. Close attention is paid to the character of the author's narrative, his approach to the historical data, choice of the events that interest him most as well as the way in which the author portraits them as mutually connected. Among the questions important for the commentary are whether he follows the wider tradition of Roman historiography or if and in which way the author's own opinions and persuasions are reflected in his work. This section also comments on the relation between Orosius'...