Relevant bibliographies by topics / JFHF

Academic literature on the topic 'JFHF'

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Published: 27 July 2024
Last updated: 28 July 2024

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Journal articles on the topic "JFHF"

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Borawski, Jason, Philip Troke, Xiaoling Puyang, Veronica Gibaja, ShanChaun Zhao, Craig Mickanin, Juliet Leighton-Davies, et al. "Class III Phosphatidylinositol 4-Kinase Alpha and Beta Are Novel Host Factor Regulators of Hepatitis C Virus Replication." Journal of Virology 83, no. 19 (July 15, 2009): 10058–74. http://dx.doi.org/10.1128/jvi.02418-08.

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ABSTRACT Host factor pathways are known to be essential for hepatitis C virus (HCV) infection and replication in human liver cells. To search for novel host factor proteins required for HCV replication, we screened a subgenomic genotype 1b replicon cell line (Luc-1b) with a kinome and druggable collection of 20,779 siRNAs. We identified and validated several enzymes required for HCV replication, including class III phosphatidylinositol 4-kinases (PI4KA and PI4KB), carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), and mevalonate (diphospho) decarboxylase. Knockdown of PI4KA could inhibit the replication and/or HCV RNA levels of the two subgenomic genotype 1b clones (SG-1b and Luc-1b), two subgenomic genotype 1a clones (SG-1a and Luc-1a), JFH-1 genotype 2a infectious virus (JFH1-2a), and the genomic genotype 1a (FL-1a) replicon. In contrast, PI4KB knockdown inhibited replication and/or HCV RNA levels of Luc-1b, SG-1b, and Luc-1a replicons. The small molecule inhibitor, PIK93, was found to block subgenomic genotype 1b (Luc-1b), subgenomic genotype 1a (Luc-1a), and genomic genotype 2a (JFH1-2a) infectious virus replication in the nanomolar range. PIK93 was characterized by using quantitative chemical proteomics and in vitro biochemical assays to demonstrate PIK93 is a bone fide PI4KA and PI4KB inhibitor. Our data demonstrate that genetic or pharmacological modulation of PI4KA and PI4KB inhibits multiple genotypes of HCV and represents a novel druggable class of therapeutic targets for HCV infection.
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Apriyanto, Dadan Ramadhan, Sri Hartati, and Beti Ernawati Dewi. "Anti-Hepatitis C virus activity of Garcinia lattissima Miq. stem barks methanol exctract." Berkala Penelitian Hayati 29, no. 3 (December 18, 2023): 95–98. http://dx.doi.org/10.23869/bphjbr.29.3.20233.

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Herbal medicine treatment for Hepatitis C Virus (HCV) infection is a promising alternative to common medical HCV treatments because it has low unwanted side effects and production costs. This study aimed to evaluate the methanol extract of Garcinia lattissima stem barks as an antiviral compound against strain JFH1 (HCV) genotype 2a. Garcinia lattissima stem bark methanolic extract (GL-SB) was added to Huh7it-1 cells infected with JFHI. Anti HCV activity was determined by Focus-forming unit (FFU) assay and the cytotoxicity activity was analyzed using the 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay. The GL-SB showed its efficacy as the anti-HCV agent with a 50% cytotoxicity concentration (CC50) of 34.2 µg/mL and a 50% effective concentration (EC50) of 4.7 µg/mL. The co-addition and post-infection phases of GL-SB showed an anti-HCV activity, according to a time-of-addition investigation. These findings imply that GL-SB might be a promising candidate for add-on therapy in the treatment of HCV infections.
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Sam, Sung Ting, Omar Sabbar Dahham, Pei Gie Gan, N. Z. Noimam, Jingi Y. Kuan, and Abdulkader M. Alakrach. "Studies on Tensile Properties of Compatibilized and Uncompatibilized Low-Density Polyethylene/Jackfruit Seed Flour (LDPE/JFSF) Blends at Different JFSF Content." Solid State Phenomena 264 (September 2017): 120–23. http://dx.doi.org/10.4028/www.scientific.net/ssp.264.120.

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Currently, natural fillers seem to be the suitable materials in polymer industry, which have emerged as a viable and abundant replacement for the relatively high-cost and non-renewable conventional fillers. However, the direct introduction of natural fillers into polymer matrix could effect negatively on some properties. Therefore, the aim of this work is to evaluate the influence of jackfruit seed flour (JFSF) (before and after compatibilization) on the tensile properties of (LDPE/JFSF) blends. Different JFSF content (5, 10, 15 and 20 wt.%) with (63-100 𝜇𝑚) particle size were prepared in this work. Twin-screw extruder at 150°C and 50rpm screw speed followed by hot-compress machine at 150°C and 10MPa pressure were used respectively to produce (LDPE/JFSF) blends. Adipic acid (AA) solution was added as a compatibilizer into all blends equally (25wt% AA into 75wt% JFSf). The changes of tensile and morphological properties were investigated. Results shown decreasing on tensile strength and elongation at break of LDPE/JFSF and LDPE/JFSF/AA as JFSF increased. In contrast, Young’s modulus increased up to 10 wt.% of JFSF and then decreased. However, the addition of Adipic acid, particularly for JFSF 5wt.% has improved the tensile properties of LDPE/JFSF blends. The SEM micrographs showed the agglomeration at high JFSF content (20 wt%) which in turn effected negatively on the tensile properties. However, the blends show homogeneous surfaces as AA added.
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Valente, Mauro, Rodolfo Figueroa, and Jorge E. Fernández. "Editorial SARX-JFMF 2018." Applied Radiation and Isotopes 157 (March 2020): 109006. http://dx.doi.org/10.1016/j.apradiso.2019.109006.

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Simister, Philip, Melanie Schmitt, Matthis Geitmann, Oliver Wicht, U. Helena Danielson, Rahel Klein, Stéphane Bressanelli, and Volker Lohmann. "Structural and Functional Analysis of Hepatitis C Virus Strain JFH1 Polymerase." Journal of Virology 83, no. 22 (September 9, 2009): 11926–39. http://dx.doi.org/10.1128/jvi.01008-09.

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ABSTRACT The hepatitis C virus (HCV) isolate JFH1 represents the only cloned wild-type sequence capable of efficient replication in cell culture, as well as in chimpanzees. Previous reports have pointed to the viral polymerase NS5B as a major determinant for efficient replication of this isolate. To understand the underlying mechanisms, we expressed and purified NS5B of JFH1 and of the closely related isolate J6, which replicates below the limit of detection in cell culture. The JFH1 enzyme exhibited a 5- to 10-fold-higher specific activity in vitro, consistent with the polymerase activity itself contributing to efficient replication of JFH1. The higher in vitro activity of the JFH1 enzyme was not due to increased RNA binding, elongation rate, or processivity of the polymerase but to higher initiation efficiency. By using homopolymeric and heteropolymeric templates, we found that purified JFH1 NS5B was significantly more efficient in de novo initiation of RNA synthesis than the J6 counterpart, particularly at low GTP concentrations, probably representing an important prerequisite for the rapid replication kinetics of JFH1. Furthermore, we solved the crystal structure of JFH1 NS5B, which displays a very closed conformation that is expected to facilitate de novo initiation. Structural analysis shows that this closed conformation is stabilized by a sprinkle of substitutions that together promote extra hydrophobic interactions between the subdomains “thumb” and “fingers.” These analyses provide deeper insights into the initiation of HCV RNA synthesis and might help to establish more efficient cell culture models for HCV using alternative isolates.
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Bungyoku, Yasuaki, Ikuo Shoji, Tatsuhiko Makine, Tetsuya Adachi, Kazumi Hayashida, Motoko Nagano-Fujii, Yoshi-Hiro Ide, Lin Deng, and Hak Hotta. "Efficient production of infectious hepatitis C virus with adaptive mutations in cultured hepatoma cells." Journal of General Virology 90, no. 7 (July 1, 2009): 1681–91. http://dx.doi.org/10.1099/vir.0.010983-0.

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Robust production of infectious hepatitis C virus (HCV) in cell culture was realized by using the JFH1 strain and the homologous chimeric J6/JFH1 strain in Huh-7.5 cells, a highly HCV-permissive subclone of Huh-7 cells. In this study, we aimed to establish a more efficient HCV-production system and to gain some insight into the adaptation mechanisms of efficient HCV production. By serial passaging of J6/JFH1-infected Huh-7.5 cells, we obtained culture-adapted J6/JFH1 variants, designated P-27, P-38 and P-47. Sequence analyses revealed that the adaptive mutant viruses P-27, P-38 and P-47 possessed eight mutations [four in E2, two in NS2, one in NS5A and one in NS5B), 10 mutations [two additional mutations in the 5′-untranslated region (5′-UTR) and core] and 11 mutations (three additional mutations in 5′-UTR, core and NS5B), respectively. We introduced amino acid substitutions into the wild-type J6/JFH1 clone, generated recombinant viruses with adaptive mutations and analysed their infectivity and ability to produce infectious viruses. The viruses with the adaptive mutations exhibited higher expression of HCV proteins than did the wild type in Huh-7.5 cells. Moreover, we provide evidence suggesting that the mutation N534H in the E2 glycoprotein of the mutant viruses conferred an advantage at the entry level. We thus demonstrate that an efficient HCV-production system could be obtained by introducing adaptive mutations into the J6/JFH1 genome. The J6/JFH1-derived mutant viruses presented here would be a good tool for producing HCV particles with enhanced infectivity and for studying the molecular mechanism of HCV entry.
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Hartati, Sri, Chie Aoki, Muhammad Hanafi, Marissa Angelina, Pratiwi Soedarmono, and Hak Hotta. "Antiviral effect of Archidendron pauciflorum leaves extract to hepatitis C virus: An in vitro study in JFH-1 strain." Medical Journal of Indonesia 27, no. 1 (May 8, 2018): 12–8. http://dx.doi.org/10.13181/mji.v27i1.2189.

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Background: Hepatitis C virus (HCV) is a leading cause of chronic liver diseases. Drug resistance to the regimen is also increasing. Hence, there is a need for new anti-HCV agents that are less toxic and more efficacious. The aim of this study is to evaluate the possibility of A. pauciflorum extracts can be a antiviral drug.Methods: Huh-7it cells were infected with the HCV genotype 2a strain JFH-I in the presence of methanol extracts of Archidenron pauciflorum. The methanol extract further partition used n-hexane, ethyl acetate, n-butanol, and water showed in which butanol extracts exerted the strongest IC50 (6.3 g/ml). Further, the butanol fraction was fractionated and yielded into 13 fractions.Results: The methanol extract of the leaves of A. pauciflorum exhibited concentration dependent inhibition against the JFH1 strain of HCV genotype 2a with an IC50 is 72.5 μg/ml. The butanol fraction exhibited the highest anti-HCV activity with an IC50 is 6.3 μg/ml. The butanol fraction was fractionated which yielded 13 fractions. Fractions 5 and 13 exhibited high anti-HCV activities with IC50 is 5.0 μg/ml and 8.5 μg/ml and a time-of-addition study demonstrated that fraction 5 inhibited viral infection at the post-entry step, whereas fraction 13 primarily inhibited the viral entry step.Conclusion: The extract A. pauciflorum can be used as a herbal-based antiviral drug.
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Upata, Maytamart, Thanyaporn Siriwoharn, Sakunkhun Makkhun, Suthasinee Yarnpakdee, Joe M. Regenstein, and Sutee Wangtueai. "Tyrosinase Inhibitory and Antioxidant Activity of Enzymatic Protein Hydrolysate from Jellyfish (Lobonema smithii)." Foods 11, no. 4 (February 21, 2022): 615. http://dx.doi.org/10.3390/foods11040615.

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The optimization of antioxidant and anti-tyrosinase activity during jellyfish hydrolysate preparation was studied using a response surface methodology (RSM) with a face-centered composite design. The influence of the hydrolysis duration and the enzyme concentration on the IC50 of the DPPH and ABTS radical scavenging activity, ferric-reducing antioxidant power (FRAP), the degree of hydrolysis (DH), yield, and the IC50 value of tyrosinase inhibitory activity were determined. The optimum conditions for the production of jellyfish hydrolysate using alcalase (JFAH), flavourzyme (JFFH), or papain (JFPH) were achieved at hydrolysis times of 360, 345, or 360 min, respectively, and at an enzyme concentration of 5.0%. JFFH had the highest antioxidant and tyrosinase inhibitory activity. JFAH, JFFH, and JFPH concentrations of 2.5 mg/mL resulted in HaCaT cells (IC80) having a survival rate of 80%. The amino acid profile of JFFH contained about 43% hydrophobic and 57% hydrophilic amino acids, comprising Gly, Cys, Glx, Asx, which were dominant. The isolation of a peptide fraction from JFFH was carried out using ultrafiltration membranes (10, 3, and 1 kDa) and gel filtration chromatography. Fraction-III (1–3 kDa) showed the highest antioxidative and tyrosinase inhibitory activity.
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Liu, Shuanghu, Ren Chen, and Curt H. Hagedorn. "Direct visualization of hepatitis C virus-infected Huh7.5 cells with a high titre of infectious chimeric JFH1-EGFP reporter virus in three-dimensional Matrigel cell cultures." Journal of General Virology 95, no. 2 (February 1, 2014): 423–33. http://dx.doi.org/10.1099/vir.0.055772-0.

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Identification of the hepatitis C virus (HCV) JFH1 isolate enabled the development of infectious HCV cell culture systems. However, the relatively low virus titres and instability of some chimeric JFH1 reporter viruses restricts some uses of this system. We describe a higher-titre JFH1-EGFP reporter virus where the NS5A V3 region was replaced with the EGFP gene and adapted by serial passage in Huh7.5 cells. Six adaptive mutants were identified: one each in E2, P7 and NS4B, plus three in the NS5A region. These adaptive mutants increased the reporter virus titres to 1×106 immunofluorescent focus-forming units ml−1, which is the highest titre of JFH1-EGFP reporter virus reported to our knowledge. This chimeric virus did not lose EGFP expression following 40 days of passage and it can be used to test the activity of HCV antivirals by measuring EGFP fluorescence in 96-well plates. Moreover, this reporter virus allows living infected Huh7.5 cells in Matrigel three-dimensional (3D) cultures to be visualized and produces infectious viral particles in these 3D cultures. The chimeric NS5A-EGFP infectious JFH1 reporter virus described should enable new studies of the HCV life cycle in 3D cell cultures and will be useful in identifying antivirals that interfere with HCV release or entry.
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Ishii, Naoto, Koichi Watashi, Takayuki Hishiki, Kaku Goto, Daisuke Inoue, Makoto Hijikata, Takaji Wakita, Nobuyuki Kato, and Kunitada Shimotohno. "Diverse Effects of Cyclosporine on Hepatitis C Virus Strain Replication." Journal of Virology 80, no. 9 (May 1, 2006): 4510–20. http://dx.doi.org/10.1128/jvi.80.9.4510-4520.2006.

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ABSTRACT Recently, a production system for infectious particles of hepatitis C virus (HCV) utilizing the genotype 2a JFH1 strain has been developed. This strain has a high capacity for replication in the cells. Cyclosporine (CsA) has a suppressive effect on HCV replication. In this report, we characterize the anti-HCV effect of CsA. We observe that the presence of viral structural proteins does not influence the anti-HCV activity of CsA. Among HCV strains, the replication of genotype 1b replicons was strongly suppressed by treatment with CsA. In contrast, JFH1 replication was less sensitive to CsA and its analog, NIM811. Replication of JFH1 did not require the cellular replication cofactor, cyclophilin B (CyPB). CyPB stimulated the RNA binding activity of NS5B in the genotype 1b replicon but not the genotype 2a JFH1 strain. These findings provide an insight into the mechanisms of diversity governing virus-cell interactions and in the sensitivity of these strains to antiviral agents.
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Dissertations / Theses on the topic "JFHF"

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Günther, Emanuel. "Entwicklung eines JPEG-Dateianalysators." Hochschule für Technik und Wirtschaft, 2021. https://htw-dresden.qucosa.de/id/qucosa%3A76129.

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Die Arbeit befasst sich mit der Verbesserung und Erweiterung eines bestehenden Softwareprojektes zum Streaming von JPEG-Bildern per RTP. In diesem Projekt werden JPEG-Bilder aus MJPEG-Dateien von einem Server zum Client übertragen. Um eine fehlerfreie Übertragung zu gewährleisten, soll zuvor die Eignung der Dateien für eine solche geprüft werden. Die entsprechenden Anforderungen finden sich in RFC 2435, welcher die Übertragung von JPEG-Bildern per RTP standardisiert. Zur Automatisierung der Überprüfung wurde diese in einem eigenen Programm implementiert. Weitere Verbesserungen wurden hinsichtlich der Ausführbarkeit des Projektes auf verschiedener Hardware getroffen. So wurden interne Algorithmen verbessert, um auch auf schwächerer Hardware einen flüssigen Ablauf zu ermöglichen. Außerdem wurde die Kompatibilität der RTSP-Implementierung im Projekt mit jener im VLC Media Player hergestellt. Zuletzt wurde das Softwareprojekt hinsichtlich der Verschlüsselung der Übertragung erweitert. Die Grundlage dafür legt die Anlayse von Anforderungen an die Verschlüsselung von Mediendaten. Es wurden zwei verschiedene Verfahren betrachtet und implementiert: Zum einen das weitverbreitete SRTP-Protokoll, zum anderen eine eigene JPEG-Verschlüsselung. Anschließend wurde die Komplexität der Entwicklung eines Verschlüsselungsverfahrens gezeigt, indem das selbst ent wickelte Verfahren durch einen Ersetzungsangriff gebrochen wurde.
This work deals with the improvement and extension of an existing software project for streaming JPEG images via RTP. In the project JPEG images are read in from MJPEG files and transmitted from a server to a client. To guarantee a faultless transmission the fitness of the files for transmission should be checked. The corresponding requirements can be found in RFC 2435 in which the transmission of JPEG images via RTP is standardized. An automation of this verification is realized in an own program. Further improvements are done in regard to the executability of the project on different hardware. Internal algorithms are improved to get a smooth execution even on weaker hardware. Additionally the compatibility of the implementation of RTSP in the project with that in the VLC Media Player is established. Finally the software project is extended in terms of the encryption of the transmission. The requirements of media data encryption are analyzed and used as the base for the following considerations. There are two operations which were examined and implemented: On the one hand the SRTP protocoll which is widely used. On the other hand an own JPEG encryption. Following that the complexity of developing an own encryption method is shown by breaking the developed JPEG encryption with a replacement attack.
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Andersson, Mikael, and Per Karlström. "Parallel JPEG Processing with a Hardware Accelerated DSP Processor." Thesis, Linköping University, Department of Electrical Engineering, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-2615.

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This thesis describes the design of fast JPEG processing accelerators for a DSP processor.

Certain computation tasks are moved from the DSP processor to hardware accelerators. The accelerators are slave co processing machines and are controlled via a new instruction set. The clock cycle and power consumption is reduced by utilizing the custom built hardware. The hardware can perform the tasks in fewer clock cycles and several tasks can run in parallel. This will reduce the total number of clock cycles needed.

First a decoder and an encoder were implemented in DSP assembler. The cycle consumption of the parts was measured and from this the hardware/software partitioning was done. Behavioral models of the accelerators were then written in C++ and the assembly code was modified to work with the new hardware. Finally, the accelerators were implemented using Verilog.

Extension of the accelerator instructions was given following a custom design flow.

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Čermák, Pavel. "Systém pro správu sbírek fotografií." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2014. http://www.nusl.cz/ntk/nusl-236037.

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This thesis deals with the management of digital photos by metadata contained in the photos. The thesis describes the structure of the formats JFIF, TIFF, RAW and EXIF format for storing metadata into photos. In the next part of this thesis is described the design and implementation of a simple photo management application. The main functionality of the application is focused on bulk editing EXIF metadata in the photos. In the conclusion of this thesis there's a proof of results and discussion about further extension options.
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Braga, Ana Claudia Silva [UNESP]. "Regulação da expressão de proteínas de choque térmico pelo vírus da hepatite C." Universidade Estadual Paulista (UNESP), 2017. http://hdl.handle.net/11449/151515.

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Submitted by ANA CLAUDIA SILVA BRAGA null (anabragga@gmail.com) on 2017-08-31T16:15:53Z No. of bitstreams: 1 Tese Doutorado Ana Claudia Silva Braga.pdf: 4552779 bytes, checksum: 456de4a6fbfd60347292d8755d6c8c47 (MD5)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
O vírus da hepatite C (HCV) causa a doença da Hepatite C e estima-se que cerca de 3% da população mundial esteja infectada com o vírus. A infecção por HCV promove a alteração na expressão de várias proteínas celulares. Estudos têm demonstrado que muitas proteínas de choque térmico (HSPs) possuem um perfil de expressão alterado na presença do vírus e algumas HSPs interagem diretamente com proteínas do HCV. Assim, o presente estudo teve como objetivo avaliar in vitro os níveis de expressão de proteínas de choque térmico na presença e ausência de HCV. Com este propósito, células de hepatoma humano Huh7.5 e células Huh7.5 infectadas com o vírus (HCV JFH-1) foram submetidas à extração de RNA e síntese de cDNA. A expressão diferencial de 84 HSPs e chaperonas foi avaliada por qPCR Array. Os resultados demonstram que cinco genes apresentaram expressão aumentada (em Log2 2), enquanto outros cinco apresentaram expressão reduzida. Para validar estes resultados os 10 genes diferencialmente expressos foram testados por qPCR em três modelos celulares para o HCV: células contendo replicon subgenômico do HCV (SGR-JFH-1), células infectadas com JFH-1 (ambos do genótipo 2a) e células contendo o replicon subgenômico S52 (genótipo 3). O gene HSPB8 mostrou expressão aumentada nos três modelos testados, condizente com os resultados obtidos por qPCR Array. Em seguida, promovemos o silenciamento de HSPB8 e foi observado um aumento na replicação viral. Em contraste, quando aumentamos a expressão de HSPB8, o HCV teve uma diminuição na taxa de replicação. O mesmo procedimento foi adotado para o gene DNAJC5B, validado no modelo viral genótipo 3, e o HCV mostrou padrão de replicação semelhante ao observado para o gene anterior. Esses resultados sugerem que HSPB8 pode atuar como um fator intracelular contra a replicação do vírus da hepatite C e DNAJC5B apresenta a mesma função, mas específico para o genótipo 3. Também avaliamos interações diretas com proteínas do HCV e os resultados demonstraram uma interação física entre a proteína NS4B de HCV e HSPB8. Esses resultados podem contribuir para uma melhor compreensão dos mecanismos envolvidos na replicação do HCV.
Hepatitis C virus (HCV) causes Hepatitis C disease and it is estimated that about 3% of world population are infected with the virus. HCV infection promotes alteration in the expression of several cellular proteins. Studies have shown that many heat shock proteins (HSPs) have an altered expression profile in the presence of the virus and some HSPs interact directly with HCV proteins. Thus, the present study aimed to evaluate in vitro the expression levels of heat shock proteins in the presence and absence of HCV. With this purpose, human hepatoma Huh7.5 cells and Huh7.5 cells infected with the virus (HCV JFH-1) were subjected to RNA extraction and cDNA synthesis. The differential expression of 84 HSPs and chaperones was assessed by qPCR Array. The results demonstrate that five genes showed increased expression (over Log2 2), while five other presented reduced expression. To validate these results, the 10 differentially expressed genes were tested by real-time PCR in three different HCV cell culture models: subgenomic HCV replicon cells (SGR-JFH-1), JFH-1 infected cells (both genotype 2a) and subgenomic S52 cells (genotype 3). The HSPB8 gene showed increased expression in all of three tested models, consistent with qPCR Array results. Then we promoted the silencing of HSPB8 and observed an increase in viral replication. In contrast, when we increased an expression of HSPB8, HCV had a decrease in replication rate. The same procedure was adopted for the DNAJC5B, validated in the viral model genotype 3, and HCV showed replication pattern similar to that observed for the previous gene. These results suggest that HSPB8 may act as an intracellular factor against hepatitis C virus replication and DNAJC5B have the same function, but genotype 3 specific. We also evaluated direct interactions with HCV proteins and the results demonstrated a physical interaction between the HCV NS4B protein with HSPB8. These results can contribute for a better understanding of the mechanisms involved in HCV replication.
FAPESP: 2013/17253-9
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Delgrange, David. "Etude de la multiplication de la souche JFH-1 du virus de l'hépatite C (VHC) en cellules Huh-7 : adaptation et sélection de mutations permettant une production rapide et massive du VHC, localisation subcellulaire de protéines du VHC, mise en évidence de clones cellulaires résistants à l'infection par le VHC." Lille 2, 2007. http://www.theses.fr/2007LIL2S011.

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Notre étude sur le virus de l'hépatite C (VHC) de génotype 2a a initialement été entreprise pour pallier le problème lié à la production virale du VHC. Ayant obtenu des titres comparables à ceux publiés, nous avons cherché à développer une approche expérimentale nous permettant de les améliorer. Pour cela, nous avons effectué des infections successives de cellules Huh-7 naïves. Nous avons montré, dans un premier temps, qu'il était possible d'obtenir des cellules Huh-7 chroniquement infectées pendant plusieurs mois. La localisation subcellulaire des protéines structurales, telles que la protéine de la capside et les glycoprotéines E1 et E2, ou de la protéine non structurale NS3 a alors été réexaminée dans le contexte d'un cycle infectieux du VHC. L'analyse de la localisation subcellulaire des protéines structurales du VHC, en immunofluorescence en microscopie confocale, a confirmé que les glycoprotéines E1 et E2 sont maintenues dans le réticulum endoplasmique des cellules infectées. Cependant, et contrairement à d'autres études, ces glycoprotéines ne s'accumulent pas dans les autres compartiments intracellulaires ou à la surface cellulaire. L'association entre la protéine de la capside et les gouttelettes lipidiques a également été confirmée. Cependant, contrairement à certaines études précédentes, la protéine C n'a pas été trouvée dans le noyau de la cellule ou en association avec des mitochondries. De façon surprenante, nous n'avons pas observé de colocalisation entre les hétérodimères E1E2 et la protéine C en cellules infectées. Par ailleurs, nous avons observé une colocalisation partielle entre la protéine de la capside et la protéine NS3, et lorsque les gouttelettes lipidiques sont révélées la protéine NS3 se retrouve autour de ces gouttelettes. Ainsi, la localisation subcellulaire des protéines structurales du VHC a pu être étudiée pour la première fois dans le contexte d'un cycle infectieux. Dans un second temps, nous avons cherché à définir si des changements dans le génome viral du clone JFH-1 pouvaient expliquer l'augmentation des titres infectieux que nous avions obtenu au cours des infections successives des cellules Huh-7. Le séquençage de l'ARN du VHC nous a permis de déterminer qu'une mutation majeure, N534K, était apparue dans la séquence codant la glycoprotéine E2 du virus. De manière intéressante, cette mutation empêche la glycosylation d'un des sites de N-glycosylation présent sur la glycoprotéine E2. En outre, lorsque cette mutation est directement introduite dans la séquence du JFH-1, elle facilite l'infection des cellules Huh-7 naïves. Dans une deuxième approche, et fort de nos résultats obtenus dans une étude portant sur des virus chimériques 1a-2a du VHC, nous avons mis en évidence que la sécrétion des particules virales du VHC de génotype 2a pouvait être améliorée par la substitution de deux acides aminés (F172C et P173S) localisés dans la séquence codant la protéine de la capside du VHC. L'insertion de ces variations (F172C, P173S et N534K) dans le génome du VHC de génotype 2a permet de produire des titres infectieux très conséquents. Ainsi, la substitution de certains acides aminés dans la séquence codant les protéines structurales permet de produire des titres infectieux du VHC importants et indépendemment de la lignée cellulaire Huh-7 utilisée. Lors de l'étude des mutants hautement productifs du clone JFH-1, nous avons observé un phénomène de cytotoxicité important sur la lignée cellulaire Huh-7. Bien que ce phénomène ne soit pas encore expliqué, nous avons été en mesure de sélectionner plusieurs clones cellulaires résistants à cet effet cytopathique. L'analyse de ces clones a montré qu'ils étaient résistants à l'infection par le VHC. Cette résistance est le résultat de la perte d'expression d'un récépteur majeur pour l'infection du VHC, la molécule de surface CD81. L'étude de ces clones cellulaires nous a permis de confirmer le rôle primordial que joue CD81 dans l'infection par le VHC.
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Mohajerani, Seyed Amir. "Immortalized human hepatocyte, an alternate model for the study of the propagation of HCV in vivo and in vitro." Master's thesis, 2010. http://hdl.handle.net/10048/1639.

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The chimeric Alb-uPA SCID mouse that has been transplanted with human hepatocytes is a model to facilitate in vivo study of HCV. We explored further development of the model by using repopulation with immortalized human hepatocytes (IHH) in place of primary human hepatocyte (PHH) transplantation to support HCV infection. In vitro HCV studies typically utilize a human hepatoma cell line (Huh7) and rely on transfection with transcribed genomic RNA derived from a unique HCV strain (JFH1). Unfortunately, this system has not been successful in support of infection with serum-derived HCV (HCVser). IHH may offer an alternative since their differentiation status remains close to that of PHH. IHH transfected with HCV RNA (H77 or JFH1) or infected with HCVser showed stable intracellular and supernatant HCV RNA by real-time RT-PCR. IHH showed intracellular HCV NS3 proteins. HCV transfected or infected IHH secrete infectious HCVcc for in vivo and vitro.
Experimental Surgery
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Books on the topic "JFHF"

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Tso, Bendi, and Marnyi Gyatso. Shépa: The Tibetan Oral Tradition in Choné. Open Book Publishers, 2023.

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Folktales of Mayotte, an African Island. Open Book Publishers, 2023.

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Book chapters on the topic "JFHF"

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Mohamad, Kamaruddin Malik, Tutut Herawan, and Mustafa Mat Deris. "Dual-Byte-Marker Algorithm for Detecting JFIF Header." In Communications in Computer and Information Science, 17–26. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-13365-7_3.

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Verma, A. K., C. Patvardhan, and C. Vasantha Lakshmi. "Robust Color Image Watermarking Scheme Using JFIF -YCbCr Color Space in Wavelet Domain." In Wireless Networks and Computational Intelligence, 187–92. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-31686-9_21.

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"JFIF (JPEG File Interchange Format)." In Encyclopedia of Multimedia, 377. Boston, MA: Springer US, 2008. http://dx.doi.org/10.1007/978-0-387-78414-4_97.

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Jesus Delfino, Kelly. "Implantação de políticas públicas para pessoas com Transtorno do Espectro Autista no Brasil." In Educação Especial e Inclusiva múltiplos olhares em interlocução. V&V Editora, 2024. http://dx.doi.org/10.47247/jfh/6063.028.4.9.

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Roberto Barbosa, Paulo, Luciano José Barbosa, and Jozeildo Kleberson Barbosa. "O direito do aluno surdo à educação: a classe hospitalar como um ambiente humanizador." In Educação Especial e Inclusiva múltiplos olhares em interlocução. V&V Editora, 2024. http://dx.doi.org/10.47247/jfh/6063.028.4.2.

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Gomes Ferreira, Elaine. "Relação entre o cerebelo e o Transtorno do Espectro Autista: uma revisão narrativa." In Educação Especial e Inclusiva múltiplos olhares em interlocução. V&V Editora, 2024. http://dx.doi.org/10.47247/jfh/6063.028.4.3.

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Paes Gonçalves Nogueira, Simone. "Desenho Universal para a Aprendizagem: uma proposta para o ensino do sistema de numeração decimal nos anos iniciais do Ensino Fundamental." In Educação Especial e Inclusiva múltiplos olhares em interlocução. V&V Editora, 2024. http://dx.doi.org/10.47247/jfh/6063.028.4.4.

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Fantato Hayakawa, Juliana. "Fala, linguagem e a interação de alunos especiais com professores em sala de aula." In Educação Especial e Inclusiva múltiplos olhares em interlocução. V&V Editora, 2024. http://dx.doi.org/10.47247/jfh/6063.028.4.7.

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Maria Donadeli, Marianna. "A formação dos professores da Prefeitura Municipal de São Paulo com base nos cursos ofertados pelo CEFAI-DRE São Miguel." In Educação Especial e Inclusiva múltiplos olhares em interlocução. V&V Editora, 2024. http://dx.doi.org/10.47247/jfh/6063.028.4.5.

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Fantato Hayakawa, Juliana. "Posfácio – educação inclusiva." In Educação Especial e Inclusiva múltiplos olhares em interlocução. V&V Editora, 2024. http://dx.doi.org/10.47247/jfh/6063.028.4.10.

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Conference papers on the topic "JFHF"

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Mohamad, Kamaruddin Malik, and Mustafa Mat Deris. "Single-byte-marker for Detecting JPEG JFIF Header Using FORIMAGE-JPEG." In 2009 Fifth International Joint Conference on INC, IMS and IDC. IEEE, 2009. http://dx.doi.org/10.1109/ncm.2009.22.

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Sim-Hui Tee and Albert Quek. "Identifying hsa-miR-122 target sites in HCV isolate JFH-1." In 2013 IEEE Business Engineering and Industrial Applications Colloquium (BEIAC). IEEE, 2013. http://dx.doi.org/10.1109/beiac.2013.6560259.

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Luo, Yilin, and Yu Sun. "An Intelligent and Interactive Gaming System to Promote Environment Awareness using Context-Based Storying." In 2nd International Conference on Machine Learning Techniques and NLP (MLNLP 2021). Academy and Industry Research Collaboration Center (AIRCC), 2021. http://dx.doi.org/10.5121/csit.2021.111414.

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Since a child, I loved to play video games, especially platform games such as Metal Slug™, Mega Man™, etc.. Therefore, I was inspired to design my own platform game; with this paper, I have the opportunity to introduce our platform game, which is a “JFF Game” we developed using Unity and Visual Studio 2019.
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Chin, Hou-Man, Darko Zibar, Nitin Jain, Tobias Gehring, and Ulrik L. Andersen. "Phase Compensation for Continuous Variable Quantum Key Distribution." In CLEO: Applications and Technology. Washington, D.C.: OSA, 2019. http://dx.doi.org/10.1364/cleo_at.2019.jf2f.1.

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Liu, Dianjing, Yixuan Tan, Erfan Khoram, and Zongfu Yu. "Training deep neural networks for the inverse design of nanophotonic structures." In CLEO: Applications and Technology. Washington, D.C.: OSA, 2019. http://dx.doi.org/10.1364/cleo_at.2019.jf2f.4.

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Hamerly, Ryan, Alex Sludds, Liane Bernstein, Marin Soljačić, and Dirk Englund. "Large-Scale Optical Neural-Network Accelerators based on Coherent Detection." In CLEO: Applications and Technology. Washington, D.C.: OSA, 2019. http://dx.doi.org/10.1364/cleo_at.2019.jf2f.5.

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Hughes, Tyler W., Momchil Minkov, Ian A. D. Williamson, Yu Shi, and Shanhui Fan. "Training of Photonic Neural Networks through In Situ Backpropagation." In CLEO: Applications and Technology. Washington, D.C.: OSA, 2019. http://dx.doi.org/10.1364/cleo_at.2019.jf3f.2.

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Cowan, Laura V., Ashley Lyons, Guillem Carles, James Babington, Andy Wood, and Andrew R. Harvey. "Combined multi-aperture 3D panoramic thermal imaging and single-photon ranging." In Computational Optical Sensing and Imaging. Washington, D.C.: OSA, 2020. http://dx.doi.org/10.1364/cosi.2020.jf2f.2.

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Hauser, Jonathan, Michael A. Golub, Amir Averbuch, Menachem Nathan, Valery A. Zheludev, and Michael Kagan. "Dual-camera snapshot spectral imaging with pupil-domain binary optical diffuser and Compressed Sensing algorithms." In Computational Optical Sensing and Imaging. Washington, D.C.: OSA, 2020. http://dx.doi.org/10.1364/cosi.2020.jf2f.3.

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Monakhova, Kristina, Kyrollos Yanny, and Laura Waller. "Snapshot hyperspectral imaging using a random phase mask and spectral filter array." In Computational Optical Sensing and Imaging. Washington, D.C.: OSA, 2020. http://dx.doi.org/10.1364/cosi.2020.jf2f.4.

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