To see the other types of publications on this topic, follow the link: IVF; In Vitro Fertilization.
Journal articles on the topic 'IVF; In Vitro Fertilization'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 journal articles for your research on the topic 'IVF; In Vitro Fertilization.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.
1
Miles, Harriet L., Paul L. Hofman, John Peek, Mark Harris, Dyanne Wilson, Elizabeth M. Robinson, Peter D. Gluckman, and Wayne S. Cutfield. "In Vitro Fertilization Improves Childhood Growth and Metabolism." Journal of Clinical Endocrinology & Metabolism 92, no. 9 (September 1, 2007): 3441–45. http://dx.doi.org/10.1210/jc.2006-2465.
Full textAbstract:
Abstract Background: There is limited information regarding the long-term outcome of children born after in vitro fertilization (IVF), although an increase in rare imprinted gene disorders such as Beckwith-Wiedemann syndrome has been reported. Methods: We recruited healthy, prepubertal children born at term after singleton pregnancy. The children in the study group were conceived using IVF with fresh embryo transfer, whereas controls were naturally conceived. Anthropometric measurements, bone age, dual-energy x-ray absorptiometry, fasting serum glucose, insulin, lipid profile, IGF-I and -II, and IGF-binding proteins 1, 2, and 3 were performed. Results: There were 69 IVF children aged 5.9 ± 0.2 yr and 71 control children aged 6.9 yr. IVF children were taller than controls when corrected for parents’ heights (height sd score of 1.05 ± 0.1 vs. 0.51 ± 0.11, P = 0.001) with higher levels of serum IGF-II (850 ± 24 vs. 773 ± 24 μg/liter, P = 0.03), higher IGF-I to IGF-binding protein 3 ratio (P = 0.04), and a trend toward higher IGF-I (105 ± 4 vs. 92 ± 4 μg/liter, P = 0.06). IVF children had higher high-density lipoprotein (1.67 ± 0.04 mmol/liter vs. 1.53 ± 0.04 mmol/liter, P = 0.02), lower triglycerides (0.65 ± 0.04 mmol/liter vs. 0.78 ± 0.04 mmol/liter, P = 0.02), and a lower total to high-density lipoprotein cholesterol ratio (2.58 vs. 2.86, P = 0.01). There were no differences in body composition. Conclusions: IVF children are taller with higher IGF-I and IGF-II levels and have a slightly more favorable lipid profile. We speculate that IVF results in epigenetic change through altered methylation of genes involved in growth and metabolism. IVF programs should consider long-term longitudinal follow-up of IVF offspring.
2
Buitendijk, Simone E. "CHILDREN AFTER IN VITRO FERTILIZATION." International Journal of Technology Assessment in Health Care 15, no. 1 (January 1999): 52–65. http://dx.doi.org/10.1017/s0266462399015160.
Full textAbstract:
This paper provides an overview of the effects of in vitro fertilization (IVF) on the children born from it. One of the main problems with IVF to date remains the high incidence of multiple pregnancies, which carry an inherent higher risk of preterm delivery and, therefore, of increased morbidity and mortality in newborns. Further, singleton pregnancies and twin pregnancies from IVF compared to control singleton or twin pregnancies appear to be at higher risk of preterm birth and low birth weight. Whether this is an effect of the procedure per se or is related to maternal factors, or a combination of both, remains to be studied. The risk of congenital malformations does not, with the available data, seem to be elevated. As of now, it remains unclear whether embryo freezing is a safe procedure. Psychomotor development of children born through IVF does not seem to be disturbed. Until further and more extensive studies are conducted, it remains unclear whether IVF poses long-term risks for the children.
3
Wiesing, Urban. "In Vitro Fertilization: Regulations in Germany." Cambridge Quarterly of Healthcare Ethics 2, no. 3 (1993): 321–26. http://dx.doi.org/10.1017/s0963180100004321.
Full textAbstract:
In Germany — as probably worldwide — in vitro fertilization (IVF) has provoked disapproval, fears, and dread, but it also raises hope and gives feelings of pride and satisfaction in a new scientific achievement. Critics look for convincing argu- ments that could ban IVF completely or at least restrict it considerably. Some of the most important arguments are outlined below.The main aspect of IVF that was new to society was that conception could take place outside the female body. Although this fact has made a deep impression on us, no ethical relevance has been attributed to it. One must look further for arguments against IVF.
4
Kolb, Bradford Alan, and Richard J. Paulson. "Unstimulated in vitro fertilization revisited." Reproductive Medicine Review 5, no. 2 (July 1996): 129–38. http://dx.doi.org/10.1017/s0962279900001289.
Full textAbstract:
The first attempts at in vitro fertilization (IVF) of human oocytes were performed during cycles utilizing human menopausal gonadotropin (hMG) and human chorionic gonadotropin (hCG). These early cycles resulted in a successful conception, which unfortunately ended as a tubal gestation. The birth of Louise Brown in 1978, the first successful IVF birth, was actually achieved following fertilization during a spontaneous cycle in which ovulation was triggered with endogenous luteinizing hormone (LH).However, due to the greater margin for error afforded by larger numbers of follicles, the practice of IVF rapidly evolved towards the use of controlled ovarian hyperstimulation (COH) to achieve higher pregnancy rates. It is easy to understand why this approach evolved. Oocyte harvesting was accomplished primarily by laparoscopy. Since oocyte yield per follicle was less than 100% and fertilization rates were limited, the relatively traumatic follicle aspiration process was more likely to result in embryo transfer if a greater number of follicles was present.
5
Källén, Bengt, Orvar Finnström, Karl-Gösta Nygren, and Petra Otterblad Olausson. "In vitro fertilization (IVF) in Sweden: infant outcome after different IVF fertilization methods." Fertility and Sterility 84, no. 3 (September 2005): 611–17. http://dx.doi.org/10.1016/j.fertnstert.2005.02.038.
Full text
6
Johnson, Martin H. "A short history of in vitro fertilization (IVF)." International Journal of Developmental Biology 63, no. 3-4-5 (2019): 83–92. http://dx.doi.org/10.1387/ijdb.180364mj.
Full textAbstract:
The history of in vitro fertilization (IVF) in humans from the early attempts in the USA through to the first definitive achievement of IVF by Edwards, Steptoe and Purdy (1969-1978), and the brief period of innovative IVF achievements to Melbourne, Australia, cut short by the passage of restrictive legislation (1979-1984) is recorded. A summary of the key achievements since the mid 1980s is then given. The shameful connotations of engaging in IVF of those esrly days is contrasted with its wide acceptance today, in which IVF is setting the norms for modern families.
7
Khan, Haroon Latif, Yousaf Latif Khan, Rameen Makhdoom, and Abdul Rahman Khawaja. "IN VITRO FERTILIZATION." Professional Medical Journal 23, no. 09 (September 10, 2016): 1138–44. http://dx.doi.org/10.29309/tpmj/2016.23.09.1711.
Full textAbstract:
Various ovarian reserve tests were developed to estimate the ovarian reserve andpredict about the outcome in subfertile females undergoing evaluation for assisted reproduction.FSH and AMH levels are considered to be good ovarian reserve indicators along with antralfollicle count. Objectives: To explore relationship of AMH and FSH in patients undergoing IVFwith respect to ovarian reserve and outcome of the treatment. Study Design: Prospective cohort.Study Period: 1st January 2015 to 31st December 2015. Place of study: Lahore Institute ofFertility and Endocrinology, Hameed Latif Hospital, Lahore Material and Methods: In 346 IVF/ICSI patients after anthropometric measurements and transvaginal ultrasound antral folliclecount were assessed in each ovary. For the hormone measurements blood samples were takenduring the early follicular phase of menstrual cycle. Clinical pregnancy was also visualizedthrough transvaginal ultrasound. Results: From the 346 IVF/ICSI patients 89 (25.79%) clinicalpregnancies resulted. The mean age in pregnant group was 32.89 ± 2.99 years and in nonpregnantgroup was 33.62 ±4.36. Mean FSH and AMH in pregnant group was 6.38 ±2.38,3.27 ±1.86 and in non- pregnant group was 7.54±3.76, 2.72 ± 1.82 respectively. Age andFSH are significantly associated with each other (p-vale = 0.000) and mostly patients had FSHbelow 9(mIU/mL). Age and AMH are significantly associated with each other (p-vale = 0.000)and mostly patients had AMH above 1.5 (ng/mL). Conclusions: Better pregnancy rate wasassociated with FSH below than 9 (mIU/mL) and AMH above 1.5 (ng/mL).
8
Heitman, Elizabeth. "SOCIAL AND ETHICAL ASPECTS OF IN VITRO FERTILIZATION." International Journal of Technology Assessment in Health Care 15, no. 1 (January 1999): 22–35. http://dx.doi.org/10.1017/s0266462399015184.
Full textAbstract:
In vitro fertilization (IVF) stands out as one of the contemporary period's most extraordinary technologies, and its social and ethical consequences among the most far reaching. Despite its uncertain effectiveness and medical consequences, IVF has contributed significantly to the medicalization of infertility and the increasingly imperative character of reproductive technology. New developments in IVF, particularly oocyte donation, have created new definitions of treatable infertility and new social needs for IVF; when the technology does not result in pregnancy or healthy babies, these developments have created profound new disappointments. IVF and the commodification of the extracorporeal embryo have also confused the social meaning and legal definition of parenthood. Ultimately the relationship between prospective parents, infertility specialists, and the embryos that they create is a highly ambiguous one. This ambiguity is likely to be a long-term characteristic of efforts to develop, use, and assess assisted reproductive technologies.
9
Bolukbas, Filiz F., Cengiz Bolukbas, Hatice Y. Balaban, Cem Aygun, Seyda Ignak, Emine Ergul, Mehtap Yazicioglu, and Suat S. Ersahin. "Intrahepatic Cholestasis of Pregnancy: Spontaneous vs in vitro Fertilization." Euroasian Journal of Hepato-Gastroenterology 7, no. 2 (2017): 126–29. http://dx.doi.org/10.5005/jp-journals-10018-1232.
Full textAbstract:
ABSTRACT Aim Intrahepatic cholestasis of pregnancy (ICP) is the most common liver disease in pregnancy. Although it was shown that multiple pregnancy and hormone therapies increase the risk of ICP, there is limited information that compared spontaneous fertilization and in vitro fertilization (IVF) from the aspect of developing ICP. In our study, we investigated the potential relationship between ICP and IVF/spontaneous pregnancy. Materials and methods We reviewed the records (between June 2007 and December 2014) of pregnancies with ICP who were referred to gastroenterology clinics in three different hospitals. Fifty-nine pregnancies (43 spontaneous fertilization, 16 IVF) with ICP were analyzed from the aspect of age, fertilization type, multiple/singleton pregnancy, delivery week, and biochemical results. Results We found that serum bile acid levels were higher in the IVF group than the spontaneous fertilization group (32.8 ± 20 vs 19.6 ± 19 μmol/L; p < 0.05). There was a significant inverse correlation between serum bile acid levels and gestational age (r = −0.42, p < 0.01) in the whole group. There was no difference between IVF and spontaneous fertilization groups in term of age, onset time of symptoms, serum alanine aminotransferase (ALT), alkaline phosphatase (ALP), total and direct bilirubin levels, prothrombin time (PT), international normalized ratio (INR), and platelet count. Conclusion Our results suggest that the serum bile acid levels are higher in IVF than in spontaneous pregnancies with ICP, but its clinical implications are not clear. Further prospective studies with large number of ICP cases are needed to clarify the effect of IVF on ICP. How to cite this article Bolukbas FF, Bolukbas C, Balaban HY, Aygun C, Ignak S, Ergul E, Yazicioglu M, Ersahin SS. Intrahepatic Cholestasis of Pregnancy: Spontaneous vs in vitro Fertilization. Euroasian J Hepato-Gastroenterol 2017;7(2):126-129.
10
Tarabrin, Roman. "Orthodox Perspectives on In Vitro Fertilization in Russia." Christian bioethics: Non-Ecumenical Studies in Medical Morality 26, no. 2 (April 16, 2020): 177–204. http://dx.doi.org/10.1093/cb/cbaa004.
Full textAbstract:
Abstract The views on in vitro fertilization (IVF) within Russian Orthodox Christian society are diverse. One reason for that variation is the ambiguity found in “The Basis of the Social Concept,” the document issued in 2000 by the Russian Orthodox Church and considered to be the primary guidelines for determining the Church’s stance on bioethics. This essay explores how the treatment of infertility reconciles with the Orthodox Christian faith and what methods of medical assistance for infertility may be appropriate for Orthodox Christians. The focus here is on IVF because it is among the most widely used methods to overcome childlessness, and the permissibility of IVF is the object of disagreement among Orthodox. The article defines criteria that can help to discern what is absolutely wrong and must be avoided from what only falls short of the mark, but not very far, for Orthodox Christians. If treatment of the underlying causes of infertility has failed or promises no hope and a husband and a wife do not feel able to carry the Cross of infertility, then from pastoral dispensation they might be blessed to use ethically acceptable variants of IVF. IVF has many variants that are different in their spiritual influence on a person. Orthodox Christians pursuing IVF should seek spiritual guidance and a blessing to pursue IVF. They must not form more embryos than will be transferred in the same cycle. Freezing, discarding, or reduction of embryos is forbidden. Infertile couples ought to use only their reproductive cells. The use of donor gametes is unacceptable. Any embryo formed ought to be transferred into the wife’s womb, and the use of surrogates is impermissible. Only a husband and wife who are able to maintain their marital union and where the wife is still of childbearing age should be blessed to use IVF.
11
Thapa, Seema, and Yun Seok Heo. "Microfluidic technology for in vitro fertilization (IVF)." JMST Advances 1, no. 1-2 (May 7, 2019): 1–11. http://dx.doi.org/10.1007/s42791-019-0011-3.
Full text
12
Vaknin-Dembinsky, Adi, Yuval Bdolah, Dimitrios Karussis, Guy Rosenthal, Panayiota Petrou, Yakov Fellig, Oded Abramsky, and Alexander Lossos. "Tumefactive demyelination following in vitro fertilization (IVF)." Journal of the Neurological Sciences 348, no. 1-2 (January 2015): 256–58. http://dx.doi.org/10.1016/j.jns.2014.11.016.
Full text
13
Bourrit, B., R. Martin-Du Pan, D. Benchouk, M. Biondo, and E. Stiksa. "Psychotic reaction after in vitro fertilization (IVF)." Journal of In Vitro Fertilization and Embryo Transfer 5, no. 2 (April 1988): 114. http://dx.doi.org/10.1007/bf01130670.
Full text
14
Winston, R. M. L. "Tubal surgery or in vitro fertilization (IVF)?" Journal of Assisted Reproduction and Genetics 9, no. 4 (August 1992): 309–11. http://dx.doi.org/10.1007/bf01203950.
Full text
15
McQueen, D. B., E. C. Feinberg, S. M. Lee, A. Schufreider, and M. Uhler. "Racial disparities in in vitro fertilization (IVF)." Fertility and Sterility 102, no. 3 (September 2014): e276. http://dx.doi.org/10.1016/j.fertnstert.2014.07.938.
Full text
16
Tehraninejad, Ensieh Shahrokh, Noushin Khazei, Elnaz Ayati, Ali Movafegh, and Omid Azimaraghi. "EFFECT OF VAGINAL SILDENAFIL ON IN VITRO FERTILIZATION SUCCESS RATES IN WOMEN WITH PREVIOUS FAILED IN VITRO FERTILIZATION ATTEMPTS." Asian Journal of Pharmaceutical and Clinical Research 11, no. 6 (June 7, 2018): 486. http://dx.doi.org/10.22159/ajpcr.2018.v11i6.25645.
Full textAbstract:
Objectives: Endometrial thickness of <9 mm is a predictor of in vitro fertilization (IVF) failure, although neither pregnancy rates nor the pregnancy outcomes are dependent on the endometrial thickness alone. The impact that uterine artery blood flow has on endometrial growth is dependent on nitric oxide which concentrations could be altered by halting a cyclic guanosine monophosphate-mediated pathway with a phosphodiesterase type 5 selective inhibitor such as sildenafil.Methods: In this clinical trial, 72 patients aged below 45 years which have had at least two earlier failed IVF attempts were randomly split into two groups each consisting of 36 patients. Both groups were started on a long IVF protocol. The case group was also administered 100 mg vaginal sildenafil suppositories daily, starting on day 3 of menstruation which was continued until human chorionic gonadotropin administration. Endometrial thickness was measured using ultrasonography in both groups plus pregnancy rates were assessed in both groups.Results: The mean age of the patients in Group A who received sildenafil; in this clinical trial, 72 patients aged below 45 years which have had at least two previous failed IVF attempts were randomly split into two groups each consisting of 36 patients was 33.8±4.8 in contrast to Group B (control group) with the mean age of 33.8±4.8. Mean endometrial thickness of 8.6±0.1 mm was recorded in Group B compared to 9.0±0.7 mm in Group A (p=0.03). Of all the 36 participants who received sildenafil citrate during the IVF cycle, 12 (33.3%) patients had successful pregnancies while 24 (66.7%) failed to get pregnant. In the control group, out of the 36 participants, 10 (27.8%) patients got pregnant while 26 (72.2%) failed the cycle (p=0.9).Conclusion: This study showed that although using vaginal sildenafil during the IVF cycle does improve endometrial thickness before implantation, this does not necessarily lead to higher pregnancy rates.
17
Lapshtaeva, A. V., I. V. Sychev, and L. N. Goncharova. "Laboratory predictors of pregnancy in vitro fertilization." Russian Clinical Laboratory Diagnostics 66, no. 5 (May 23, 2021): 291–96. http://dx.doi.org/10.51620/0869-2084-2021-66-5-291-296.
Full textAbstract:
Identification of factors determining both of favorable and unfavorable outcome of IVF will increase the effectiveness of this method and optimize infertility treatment. The aim of the research is to analyze the correlation between serum IL-1α concentration, its gene rs1800587 (C/T) genotype carrier and thyroid-stimulating hormone (TSH), thyroid hormones (triiodothyronine (T3) and tetraiodothyronine (T4)), and evaluate the prognostic significance of their combinations in women with tube-peritoneal infertility under the IVF program. 120 patients with tube-peritoneal infertility who applied for an IVF program were examined. Depending on the outcome of the procedure, 2 groups of patients were allocated: 1 group - 40 women who had a pregnancy after IVF, 2 group - 80 patients who did not have a pregnancy. The content of IL-1α, TSH, T3, T4 was determined in blood by ELISA. Genotyping was performed on the rs1800587 (C/T) polymorphic marker of the IL-1α gene. TSH, T3, T4 were within the norm for both groups. In our study, women with a TSH concentration of 0.23 to 1.7 nmol/L had a chance of a favorable IVF outcome 1.4 times higher than with other TSH levels (p = 0.042901); with a T3 level of 1.0 to 1.8 nmol/L had a chance of becoming pregnant 5.7 times higher than with other levels of T3 (p = 0.00002). For T4 concentration, the confidence test was not achieved (p = 0.068505). The individual indicators of IL-1α, TSH, T3 and carrier of the genotype of the gene IL-1α at the preconceptive stage have lower diagnostic value than their combined combination. Three combinations have maximum predictive value: a combination of the T/T genotype of the IL-1α gene and the TSH level of 0.23 to 1.7 nmol/l - OR = 8.1 (p = 0.000048); combination of IL-1α of 28.7 to 85.1 pg/ml, T/T gene genotype IL-1α and TSH level of 0.23 to 1.7 nmol/l - OR = 8.1 (p = 0.000048); combination of IL-1α of 28.7 to 85.1 pg/ml, T/T gene genotype IL-1α, TSH level of 0.23 to 1.7 nmol/l and T3 level of 1.0 to 1.8 nmol/l - OR = 8.1 (p = 0.000146). Thus, proposed new prognostic markers of IVF program effectiveness.
18
Elm, Laura. "Embryo Mortality and In Vitro Fertilization." Ethics & Medics 43, no. 4 (2018): 3–4. http://dx.doi.org/10.5840/em20184347.
Full textAbstract:
This essay hopes to convey the problematic framework of outcomes for assisted reproductive technologies (ARTs) as they are published today, reframe the logic to focus on human embryo mortality, and quantify a population of human beings generally not accounted for in publicly available in-vitro fertilization data. This analysis, conducted using the CDC’s 2015 National Summary data in conjunction with a retrospective study conducted by one of the nation’s largest ART delivery systems, is one of the first attempts to estimate pre-transfer embryo mortality in IVF. This analysis focuses on fresh non-donor cycles only, that is, rounds of IVF treatment that include immediate transfer of at least one embryo and in which the embryos transferred were engendered using the recipient’s own eggs.
19
Sotskiy, P. O., L. G. Gevorkyan, O. L. Sotskaya, and M. D. Safaryan. "Drug resistant miliary tuberculosis after in vitro fertilization." Tuberculosis and Lung Diseases 99, no. 5 (June 11, 2021): 57–62. http://dx.doi.org/10.21292/2075-1230-2021-99-5-57-62.
Full textAbstract:
The article describes a clinical case of drug resistant miliary tuberculosis in an immunocompetent woman with a multifetal pregnancy after in vitro fertilization (IVF). Difficulties in diagnosing miliary tuberculosis were due to the late detection of the initial focus of infection, that was endometrial tuberculosis, which generalized after IVF. The rare association of isthmic nodose salpingitis and a borderline ovarian tumor (serous ovarian cystadenoma) was a factor complicating the detection of tuberculosis.Conclusion: In an IVF program with bilateral fallopian tube occlusion, tuberculosis should be considered as a possible cause of this pathology. Endometrial tuberculosis is associated with a risk of repeated implantation failure and generalization of the disease.
20
Doronicheva, D. A., and N. S. Stuleva. "Key milestones in the history of in vitro fertilization." Obstetrics, Gynecology and Reproduction 14, no. 2 (July 26, 2020): 246–51. http://dx.doi.org/10.17749/2313-7347/ob.gyn.rep.2020.115.
Full textAbstract:
Here, we discuss essential milestones in developing and establishing method in vitro fertilization (IVF). Moreover, we also outline most prominent breakthroughs and discoveries performed in diverse time, each of which brought mankind closer to emergence of the first successful results after applying IVF in human. In addition, we also consider a history of IVF introduced in Russia as well as current issues requiring to be solved in the nearest future.
21
WILLIAMS, LINDA S. "Adoption Actions and Attitudes of Couples Seeking In Vitro Fertilization." Journal of Family Issues 13, no. 1 (March 1992): 99–113. http://dx.doi.org/10.1177/019251392013001007.
Full textAbstract:
This exploratory study examines the adoption actions and attitudes of 16 childless women, and the husbands of 14 of these women, who applied for or underwent in vitro fertilization (IVF) in Ontario, Canada, between 1984 and 1987. Interview data reveal that IVF and adoption were sought concurrently by most of these couples and that wives were more in favor of adoption than were husbands. The author concludes that the continued importance of the biological tie between parent and child plus the current lack of “adoptable” babies will ensure that the demand for IVF remains high. However, unless the success rate of IVF increases dramatically, IVF couples will also continue to seek adoption.
22
Bloise, Enrrico, Wingka Lin, Xiaowei Liu, Rhodel Simbulan, Kevin S. Kolahi, Felice Petraglia, Emin Maltepe, Annemarie Donjacour, and Paolo Rinaudo. "Impaired Placental Nutrient Transport in Mice Generated by in Vitro Fertilization." Endocrinology 153, no. 7 (May 4, 2012): 3457–67. http://dx.doi.org/10.1210/en.2011-1921.
Full textAbstract:
More than 4.5 million children have been conceived by in vitro fertilization (IVF). Interestingly, singleton IVF offspring born at term have an increased incidence of low birth weight. The mechanism responsible for the lower birth weight is unknown, but alterations in placental function are possible. Hence, the goal of our study was to examine placental growth and function in mice generated in vivo or in vitro. To assess placental function, blastocysts were generated by IVF or produced by natural mating (control group); both IVF and control blastocysts were transferred to pseudopregnant recipients. Placental weights did not differ at embryonic d 15.5 (E15.5) but were increased at E18.5 in the IVF group (25.4%, P < 0.001) compared with control. Proliferation was increased in IVF placentae, whereas overall placental gross morphology and apoptosis were not affected. Both fetal weights (16.4% lower at E15.5 and 8.8% lower at E18.5, P < 0.05) and fetal to placental ratios were lower (P < 0.001) in the IVF compared with the control group at both time points, whereas birth weights did not differ. At E18.5, the mRNA for selected glucose, system A amino acid transporters, and imprinted genes were down-regulated in IVF placentae. GLUT3 protein level was decreased in the IVF group (P < 0.05). Importantly, intrajugular injections of 14C-methyl-d-glucose or 14C-MeAIB tracers (n = 6 litters per group) showed that placental transport of glucose and amino acids were 24.8% (not significant) and 58.1% (P < 0.05) lower in the IVF group. Fetal accumulation of glucose was not different, but amino acid accumulation was significantly (36 %) lower in IVF fetuses (P < 0.05). We conclude that IVF alters both fetal and placental growth and, importantly, decreases placental transport efficiency in mice conceived by IVF.
23
Zheng, Cuihong, Xiaoyang Wan, Xiaohu Xu, Mingmin Zhang, Guangying Huang, and Wei Wang. "General Issues in Clinical Research of Acupuncture and In Vitro Fertilization." Evidence-Based Complementary and Alternative Medicine 2020 (April 13, 2020): 1–5. http://dx.doi.org/10.1155/2020/3460641.
Full textAbstract:
In vitro fertilization-embryo transfer (IVF-ET), a well-developed technology, provides the last possibility or hope for infertile women. However, the live birth rate per IVF cycle is still not satisfactory. Acupuncture is a frequently used adjunctive therapy drawing wide attention on improvement of IVF. Although so many randomized controlled trials have been evaluating the effect of acupuncture on IVF in the past 20 years, the exact efficacy of acupuncture on IVF is still controversy mainly because of no consensus on placebo control and acupuncture scheme. This paper focused on the general issues in clinical research of acupuncture and IVF and gave some suggestions for future directions.
24
Hill, Micah J., Steve Hong, and John L. Frattarelli. "Body Mass Index Impacts In Vitro Fertilization Stimulation." ISRN Obstetrics and Gynecology 2011 (October 27, 2011): 1–5. http://dx.doi.org/10.5402/2011/929251.
Full textAbstract:
The objective of the study was to prospectively determine if body mass index (BMI) is predictive of live birth rates in patients undergoing IVF. The prospective study enrolled 117 infertility patients with the primary outcome measure being IVF success rates. Mean BMI did not differ between patients with successful outcomes and those without successful outcomes. There was a significant positive correlation between BMI and the number of stimulated follicles (r=0.19, P<.05). A significant negative correlation between BMI and ampules of gonadotropins used (r=−0.25, P<.01) and between BMI and days of stimulation (r=−0.19, P<.05) was noted. These data demonstrate that women with an elevated BMI produce more follicles, stimulate quicker, and require less gonadotropins during IVF. However, BMI did not have a significant effect on pregnancy outcome rates.
25
Sakka, Sophia D., Ariadne Malamitsi-Puchner, Dimitrios Loutradis, George P. Chrousos, and Christina Kanaka-Gantenbein. "Euthyroid Hyperthyrotropinemia in Children Born after in Vitro Fertilization." Journal of Clinical Endocrinology & Metabolism 94, no. 4 (April 1, 2009): 1338–41. http://dx.doi.org/10.1210/jc.2008-1624.
Full textAbstract:
Abstract Context: Assisted reproduction techniques are now commonly used. Although classic in vitro fertilization (IVF) started almost 30 yr ago, few long-term systematic prospective studies of children conceived with assisted reproduction have been performed. Objective: Our objective was to investigate thyroid function in children conceived after IVF vs. naturally conceived controls. Populations and Methods: A total of 106 children conceived after classic IVF and 68 naturally conceived controls, aged 4–14 yr, were studied. All children were thoroughly examined, and serum T3, T4, TSH, anti-thyroid peroxidase, and anti-thyroglobulin antibodies were measured. A second TSH determination and a thyroid ultrasound were performed for TSH higher than 5 μIU/ml, and children were considered to have persistent hyperthyrotropinemia, if the TSH elevation was confirmed. Results: Seven IVF children but none of the controls had persistent elevations of circulating TSH, suggesting euthyroid hyperthyrotropinemia or subclinical primary hypothyroidism (P = 0.044). TSH was significantly higher in the IVF group than in controls (P = 0.046), whereas no significant differences in the concentrations of T3 or T4 were observed. None of the children had detectable circulating antithyroid antibodies in either group. Conclusions: A significant elevation of serum TSH compatible with a mild TSH resistance of the thyroid were found in IVF children compared with controls. This was not due to the presence of antithyroid autoantibodies. We suggest that this might represent a slight epigenetic developmental abnormality related to the preimplantation manipulation of the embryo. Further studies are needed to confirm these findings and to better determine their etiopathogenesis and clinical significance.
26
Pongsuthirak, Pallop. "The effect of insemination methods on in vitro maturation outcomes." Clinical and Experimental Reproductive Medicine 47, no. 2 (June 1, 2020): 130–34. http://dx.doi.org/10.5653/cerm.2019.03300.
Full textAbstract:
Objective: The aim of this study was to compare the effects of conventional insemination (<i>in vitro</i> fertilization [IVF]) and intracytoplasmic sperm injection (ICSI) on the fertilization, developmental competence, implantation potential, and clinical pregnancy rate of embryos derived from <i>in vitro</i> matured oocytes of patients with polycystic ovary syndrome (PCOS).Methods: A prospective study was carried out among 38 PCOS patients who had undergone <i>in vitro</i> maturation (IVM) treatment. In total, 828 immature oocytes were collected from 42 cycles and randomly assigned for insemination by IVF (416 oocytes) or ICSI (412 oocytes). After fertilization, the embryos were cultured until the blastocyst stage and single embryos were transferred after endometrial preparation and under ultrasound guidance.Results: No significant differences were found in the maturation rate (78.1% vs. 72.6% for IVF and ICSI insemination, respectively; <i>p</i>= 0.076), fertilization rate (59.4% vs. 66.9% for IVF and ICSI insemination, respectively; <i>p</i>= 0.063), or the formation of good-quality blastocysts (40.9% vs. 46.5% for IVF and ICSI insemination, respectively; <i>p</i>= 0.314). Implantation and clinical pregnancy also did not show significant differences.Conclusion: There was a comparable yield of <i>in vitro</i> matured oocytes derived from PCOS patients in terms of fertilization, blastocyst formation, implantation rate, and clinical pregnancy between IVF and ICSI insemination. These findings provide valuable insights for choosing assisted reproductive treatment in women with PCOS, as IVM offers promising outcomes and is less invasive and less costly.
27
Overall, Christine. "Access to In Vitro Fertilization: Costs, Care and Consent." Dialogue 30, no. 3 (1991): 383–98. http://dx.doi.org/10.1017/s0012217300011732.
Full textAbstract:
What would be a genuinely caring approach to the provision of procedures of so-called artificial reproduction such as in vitro fertilization (IVF)? What are appropriate and justified social policies with respect to attempting to enable infertile persons to have offspring? These urgent questions have provoked significant disagreements among theologians, sociologists, healthcare providers, philosophers and even — or especially — among feminists. In the existing literature and in developing social policy, three different kinds of answers can be discerned. (1) Some have suggested that access to IVF should be provided as a matter of right. (2) Some existing social policies and practices imply that access to IVF is a privilege. (3) Some theorists have argued that, because of its alleged violation of family values and marital security, or because of its risks, costs, and low success rate, IVF should not be available at all. After evaluating each of these views, I shall offer a feminist alternative, describing what I think would constitute the caring provision of in vitro fertilization.
28
Van Wissen, Barbara, Claude Eisenberg, Pascale Debey, Gilles Pennehouat, Jacques Auger, and Ondine Bomsel-Helmreich. "In vitro DNA fluorescence after in vitro fertilization (IVF) failure." Journal of Assisted Reproduction and Genetics 9, no. 6 (December 1992): 564–71. http://dx.doi.org/10.1007/bf01204255.
Full text
29
Serita, Y., C. Kubota, and T. Kojima. "221 EFFECT OF ROCKING ON IN VITRO PRODUCTION OF BOVINE EMBRYOS." Reproduction, Fertility and Development 21, no. 1 (2009): 209. http://dx.doi.org/10.1071/rdv21n1ab221.
Full textAbstract:
This study tested whether embryo development yield using in vitro fertilization (IVF) could be improved by rocking cultures. Bovine ovaries were obtained at a slaughterhouse and transported to the laboratory within 6 h. Cumulus–oocyte complexes were collected and 20–25 were transferred in 100-μL drops of TCM-199 containing 10% fetal bovine serum and antibiotics under paraffin oil. Maturation was for 20–24 h at 38.5°C under 5% CO2 and 95% air in a humid atmosphere (IVM). In vitro fertilization was carried out for 6 h using frozen–thawed sperm from a single bull in modified Brackett and Oliphant (BO) medium. Presumptive zygotes were cultured in CR1aa supplemented with 10 mg mL–1 of BSA or 5% FBS for 9 d at 38.5°C under 5% CO2, 5% O2, and 90% N2 in a humid atmosphere (IVC). Rocking was performed to a height of 6 cm every 7 s using a Profile Rocker (New Brunswick Scientific Co., Edison, NJ, USA) in an incubator. Dishes were placed at a 15-cm distance from the fulcrum of the rocker. The conventional method (no rocking) served as a control, and every experiment was replicated 3 times. For Experiment 1, the effect of the period of rocking on developmental competence was examined when COC or zygotes were subjected to rocking for IVM, IVF, or IVC (IVM-move, IVF-move, and IVC-move). There were no significant differences in rates of oocyte maturation, cleavage, and development for IVM-move v. the control, or for rate of development between IVC-move and the control. However, the rate of fertilization for IVF-move was higher than that of the control (88.9 v. 67.5%; P < 0.01), and the rate of development was higher for IVF-move than for the control (39.0 v. 25.7%; P < 0.05). For Experiment 2, the effect of rocking frequency during IVF on development was determined. The IVF cultures were rocked every 7, 3.5, and 1.5 s (IVF-1move, IVF-2move, IVF-3move). The rates of cleavage on IVF-1move, IVF-2move, IVF-3move, and the control were 74.3, 69.8, 68.8, and 60.4%, and the rates of development were 39.0, 48.3, 26.2, and 25.7%, respectively. The rates of development on IVF-1move and IVF-2move were significantly different from the control and IVF-3move (P < 0.01). These results showed that rocking during IVF improved fertilization and embryo yield, and that frequency of rocking affected embryo development.
30
Weinerman, Rachel. "In vitro fertilization (IVF): Where are we now?" Birth Defects Research 110, no. 8 (May 1, 2018): 623–24. http://dx.doi.org/10.1002/bdr2.1227.
Full text
31
Pieters, M. H. E. C., J. P. M. Geraedts, J. C. M. Dumoulin, J. L. H. Evers, M. Bras, F. H. A. C. Kornips, and P. P. C. A. Menheere. "Cytogenetic analysis of in vitro fertilization (IVF) failures." Human Genetics 81, no. 4 (March 1989): 367–70. http://dx.doi.org/10.1007/bf00283693.
Full text
32
Yovich, John L., and J. G. Grudzinskas. "Semantics and acronyms in in vitro fertilization (IVF)." Journal of In Vitro Fertilization and Embryo Transfer 4, no. 5 (October 1987): 296–97. http://dx.doi.org/10.1007/bf01555210.
Full text
33
Costantini-Ferrando, M. F., M. Joseph-Sohan, E. Grill, E. Rauch, and S. D. Spandorfer. "Does stress affect in vitro fertilization (IVF) outcome?" Fertility and Sterility 106, no. 3 (September 2016): e61. http://dx.doi.org/10.1016/j.fertnstert.2016.07.1078.
Full text
34
Kalashnikov, S. A. "Specific characteristics of multiple pregnancies after in vitro fertilization." Voprosy ginekologii, akušerstva i perinatologii 19, no. 5 (2020): 114–23. http://dx.doi.org/10.20953/1726-1678-2020-5-114-123.
Full textAbstract:
Multiple pregnancy is currently considered an important social and research problem, because it is often associated with a number of complications, such as premature birth and extremely low birth weight. Multiple pregnancies are facilitated by late reproductive age of the mother and in vitro fertilization (IVF) with the transfer of several embryos. In this review, we analyze specific characteristics of multiple pregnancies after IVF. Key words: twins, neonatal outcomes, in vitro fertilization
35
Islam, Sharmin, Rusli Bin Nordin, and Hanapi Bin Mohd. "Ethical considerations on in vitro fertilization technologies in Bangladesh." Bangladesh Journal of Medical Science 12, no. 2 (May 13, 2013): 121–28. http://dx.doi.org/10.3329/bjms.v12i2.14938.
Full textAbstract:
This paper aims to address ethical issues related to the assisted reproductive technology method in vitro fertilization (IVF) from the Islamic Shar?ah. This review reflects that the Islamic ethical viewpoint IVF is rational, comprehensive, and aims at the greatest good of mankind. Bangladesh Journal of Medical Science Vol. 12 No. 02 April13 Page 121-128 DOI: http://dx.doi.org/10.3329/bjms.v12i2.14938
36
Lerner-Geva, L., E. Geva, J. B. Lessing, A. Chetrit, and A. Amit. "The possible association between in vitro fertilization treatments and cancer development." International Journal of Gynecologic Cancer 13, no. 1 (2003): 23–27. http://dx.doi.org/10.1136/ijgc-00009577-200301000-00004.
Full textAbstract:
The objective of this paper is to assess whether ovarian hyperstimulation and in vitro fertilization (IVF) are associated with increased risk of cancer development, using an historical cohort analysis of infertile women who attended the IVF unit, Lis Maternity Hospital Tel Aviv Medical Center, Tel Aviv, Israel. One thousand and 82 women participated in the IVF treatment program between 1984 and 1992. Cancer incidence rates were determined through the National Cancer Registry and were compared to the expected rates with respect to appropriate age and continent of birth. Twenty-one cases of cancer were observed as compared to 11 that were expected (SIR 1.91; 95% CI 1.18–2.91). When cancer cases that were diagnosed within one year of the IVF treatment were excluded from the analysis (SIR = 1.46; 95% CI 0.83–2.36), no significant excess risk of cancer was noted. We conclude that in this cohort of infertile women, the higher than expected cancer rate could not be attributed to IVF treatments. Special attention should be made to women who may be diagnosed with cancer during or shortly after IVF treatment.
37
Zhang, M., X. W. Liang, Y. Q. Lu, and K. H. Lu. "353 IN VITRO FERTILIZATION WITH FLOW-SORTED BUFFALO SPERM." Reproduction, Fertility and Development 18, no. 2 (2006): 283. http://dx.doi.org/10.1071/rdv18n2ab353.
Full textAbstract:
Flow cytometrically sorted X and Y sperm have been successfully used for IVF and the production of offspring in cattle and sheep (Maxwell et al. 2004 Anim. Reprod. Sci. 82–83, 79–95).The objective of this study was to test the feasibility of flow sorted buffalo sperm used in IVF systems and to establish a suitable IVF system for sorted buffalo sperm. Oocytes were aspirated from 2–6 mm follicles on the buffalo ovaries from a slaughterhouse and matured for 22–24 h in a 1-mL dish containing TCM199 + 10% OCS + 3% BFF (bovine folliciular fluid) + hormones at 38.5°C, 5% CO2 in air with maximum humidity. Semen was sorted by a flow-sorter (Clontech, Mountain View, CA, USA) into X- and Y-chromosome bearing sperm at 90% accuracy and stored at 4°C for later use with IVF. Sorted sperm were prepared for IVF by centrifugation (700g, 20 min) through a Percoll gradient (90%:45%), and washed (centrifugation at 700g, 5 min) in mTALP-BSA. For IVF, groups of 10–15 oocytes were transferred to 40-μL drops of mTALP-BSA and incubated with motile sperm at a concentration of 2 × 106 sperm mL−1 in each fertilization drop for 8–10 h under mineral oil. Presumptive zygotes were cultured until Day 8 in 25-µL drops of TCM–199 supplemented with 0.33 mM pyruvate and 10% NCS with granulosa cells at 38.5°C under 5% CO2 in air. Cleavage and blastocyst rates per oocyte insemination were recorded on Day 2 and Days 6–8 after insemination, respectively. Data were analyzed by ANOVA procedures with replicates and treatments in the model. There were significant differences in cleavage rate (42.23% vs. 52.28%) and blastocyst rate (20.62% vs. 27.67%) between sorted and unsorted sperm, respectively (Table 1). There were no significant differences in the proportions of blastocyst development rates on Days 6, 7, or 8 after insemination with sorted and unsorted sperm. The results indicate that sorted buffalo sperm from two bulls have been successfully used in an IVF system to produce sex-controlled embryos. Table 1. Cleavage and blastocyst rates with different sperm types This research was supported by grants from the National Natural Science Foundation of China (30360073) and the Guangxi Department of Science and Technology (0330004–13). The authors (M. Z. and X.W. L.) contributed equally to this work.
38
Abdelgelel, Gehan S., Shadia H. Muhsib, Mona H. Abdelaal, and Randa M. Ibrahim. "Effect of Nursing Guidelines on Coping of Infertile Couples Undergoing In Vitro Fertilization." Evidence-Based Nursing Research 2, no. 2 (May 12, 2020): 11. http://dx.doi.org/10.47104/ebnrojs3.v2i2.126.
Full textAbstract:
Context: Infertility is defined as not being able to conceive after one year of unprotected sex. In vitro fertilization (IVF) is a process of fertilization where an ovum is combined with sperm outside the body, in vitro. In vitro fertilization (IVF) is psychologically and emotionally stressful. Coping strategies are needed to master, tolerate, reduce, or minimize stressful events.
Aim: This study aimed to evaluate the effect of nursing guidelines on coping of infertile couples' undergoing In Vitro Fertilization.
Methods: A quasi-experimental research design was utilized to achieve the aim of this study. This study conducted at the assisted reproductive technology unit of Ain shams Maternity University Hospital on a convenient sample of 98 couples undergoing fertility treatments. Two tools were used for data collection; the first tool was a structured interviewing questionnaire to assess the couple's socio-demographic data, obstetric history, the couple's knowledge regarding in vitro fertilization. The second tool was ways of coping scale (WQS) to assess coping strategies among the infertile couple.
Results: There is no statistically significant difference between couples in both groups according to their knowledge and their coping strategies to IVF before the implementation of nursing guidelines (p> 0.05). In contrast, there is a highly statistically significant improvement in knowledge and coping strategies of couples on the study group compared to control group couples after implementation of nursing guidelines(p<0.001).
Conclusion: The finding of the current study supported the hypothesis, which stated that the infertile couples who will expose to the nursing guidelines, will exhibit improved coping strategies to IVF compared to the controls. The study recommended the application of nursing guidelines at the IVF unit of Ain Shams Maternity University Hospital and other settings for IVF treatment as routine care to improve infertile couples' coping strategies.
39
Corabian, Paula, and David Hailey. "THE EFFICACY AND ADVERSE EFFECTS OF IN VITRO FERTILIZATION AND EMBRYO TRANSFER." International Journal of Technology Assessment in Health Care 15, no. 1 (January 1999): 66–85. http://dx.doi.org/10.1017/s0266462399015172.
Full textAbstract:
This paper examines the current status of in vitro fertilization and embryo transfer (IVF-ET) as a treatment for various types of infertility. We reviewed studies on the efficacy and safety of IVF-ET and intracytoplasmic sperm injection (ICSI) plus IVF-ET, compared with conventional treatment or no treatment for various infertility diagnoses. Material retrieved included English language publications between 1992 and January 1997 that reported the results of prospective controlled clinical trials, cohort studies, and retrospective comparative studies with large series, and reviews presenting risks, complications, and longer-term health consequences associated with IVF-ET and ICSI. No adequate prospective comparative studies of sufficient power on the use of IVF-ET for specific infertility diagnoses have been reported to date. Most of the published reports concerning results with IVF-ET as a treatment of infertility have been based upon small, uncontrolled studies, with various methodological weaknesses. Reported results are not directly comparable. There are few follow-up data on outcomes after pregnancy is established or on long-term health consequences of the use of IVF-ET on mothers and their babies. IVF-ET has diffused widely without comprehensive assessment of its efficacy and safety. The available evidence supports its use only for severe bilateral tubal occlusion. For other diagnoses of infertility the evidence is limited and does not establish whether IVF-ET is effective. Long-term, well-designed, prospective clinical trials are required to determine when and for what indications IVF-ET is effective and what its health effects are on both mothers and their babies.
40
Trninic-Pjevic, Aleksandra, Vesna Kopitovic, Sonja Pop-Trajkovic, Artur Bjelica, Irena Bujas, Dunja Tabs, Djordje Ilic, and Dragan Stajic. "Effect of hysteroscopic examination on the outcome of in vitro fertilization." Vojnosanitetski pregled 68, no. 6 (2011): 476–80. http://dx.doi.org/10.2298/vsp1106476t.
Full textAbstract:
Bacground/Aim. Implantation failure after embryo transfer is one of the main problems of in vitro fartilization (IVF) and intrauterine pathologies can lead to unsuccessful outcome. The aim of this study was to determine if hysteroscopic examination of uterine cavity and consequent treatment of intrauterine lesions prior to IVF could improve the pregnancy rate in women under 38. Methods. This study included 480 patients under 38, who had undergone IVF or IVF\ICSI - embryo transfer cycles, in which one or more good quality embryos were transferred. By transvaginal sonography performed within the past 2 months, the uterus was found normal in all the patients enrolled in our IVF unit. The patients were divided into three groups: group A - with no hysteroscopic evaluation and no pathology, group B - with hysteroscopy but no pathology, and group C - with abnormal hysteroscopy finding and corresponding treatment. Results. The obtained results revaled no difference in the mean age, duration of infertility, number of mature oocytes in either group (p > 0.05). Clinical pregnancy rates in the groups A, B and C were 36.9%, 58.75% and 32.7%, respectively, and delivery rates were 27.5%, 48.7% and 25.7%, respectively. There was a statistically significant difference among the groups concerning pregnancy and delivery rates. Conclusion. Considering the results of this study we could conclude that hysteroscopy, as a routine examination, should be performed before the first IVF-ET cycle in all patients thereby reducing the failures and then the costs of IVF-ET.
41
Xue, Lin-Tao, Rui-Xue Wang, Bing He, Wei-Ying Mo, Li Huang, Shi-Kai Wang, Xian-Bao Mao, Jun-Ping Cheng, Yue-Yue Huang, and RuiZhi Liu. "Effect of sperm DNA fragmentation on clinical outcomes for Chinese couples undergoing in vitro fertilization or intracytoplasmic sperm injection." Journal of International Medical Research 44, no. 6 (November 11, 2016): 1283–91. http://dx.doi.org/10.1177/0300060516664240.
Full textAbstract:
Objective To investigate the effect of sperm DNA fragmentation on the fertilization rate, embryo development and pregnancy outcome of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in a cohort of Chinese couples. Methods Infertile couples that had undergone assisted reproductive technology at our centre between January 2011 and December 2013 were included in this retrospective study. Fractions of prepared sperm samples were evaluated for sperm DNA fragmentation on the day of oocyte recovery. Results Of the 550 couples selected, 415 had undergone IVF and 135 ICSI. Sperm DNA fragmentation rate was significantly negatively correlated with the fertilization rate in the ICSI cycles but not the IVF cycles. No association was found between sperm DNA fragmentation and cleavage rate or good quality embryo formation rates in IVF or ICSI cycles. Receiver operating characteristic (ROC) curve analysis showed that the sperm DNA fragmentation rate was a statistically significant prognostic indicator of the clinical fertilization rate in ICSI cycles; a rate > 22.3% was associated with a lower fertilization rate following ICSI compared with a rate ≤ 22.3%. Conclusions High values of sperm DNA fragmentation were associated with a low fertilization rate following ICSI but were not associated with alterations in pregnancy or live birth rates in either ICSI or IVF in this cohort of Chinese couples.
42
Afaf T, Elnashar, and Sabry Mohamed. "Chronic endometritis in in vitro fertilization failure patients." Clinical Journal of Obstetrics and Gynecology 3, no. 2 (December 1, 2020): 175–81. http://dx.doi.org/10.29328/journal.cjog.1001073.
Full textAbstract:
Introduction: Chronic endometritis (CE) is a common cause of infertility in asymptomatic patients and its diagnosis and treatments improved assisted reproduction technique outcome in most of the specialized centers. Diagnosis of CE in endometrial biopsy by Hematoxylin and Eosin (H&E) stain is hard to identify chronic inflammatory cells from the stroma and the use of plasma cells-specific stains is helpful. Aim of the work: Evaluation of the use of CD138 in the identification of plasma cells in endometrial biopsy of patients with previous IVF trial failure. Material and methods: Hysteroscopic and curettage endometrial biopsies from fifty-five females with previous IVF trial failure were stained with H&E and CD138 immunostaining for detection of plasma cells. Results: Plasma cells were identified in 52.7% of cases by H&E and in 6/55 by CD138 immunostaining. CD138 is more sensitive in detecting plasma cells in endometrial biopsy than H&E stain. There was a significant statistical correlation between CE and abnormal uterine bleeding, abortion and primary infertility (p > 0.5). Conclusion: Diagnosis of CE is helpful in infertility patients with IVF trial failure to improve the outcome of the maneuver. CD138 is more sensitive for plasma cells specially in endometrial biopsies than H&E.
43
Afaf T, Elnashar, and Sabry Mohamed. "Chronic endometritis in in vitro fertilization failure patients." Clinical Journal of Obstetrics and Gynecology 3, no. 2 (December 1, 2020): 175–81. http://dx.doi.org/10.29328/journal.cjog.1001073.
Full textAbstract:
Introduction: Chronic endometritis (CE) is a common cause of infertility in asymptomatic patients and its diagnosis and treatments improved assisted reproduction technique outcome in most of the specialized centers. Diagnosis of CE in endometrial biopsy by Hematoxylin and Eosin (H&E) stain is hard to identify chronic inflammatory cells from the stroma and the use of plasma cells-specific stains is helpful. Aim of the work: Evaluation of the use of CD138 in the identification of plasma cells in endometrial biopsy of patients with previous IVF trial failure. Material and methods: Hysteroscopic and curettage endometrial biopsies from fifty-five females with previous IVF trial failure were stained with H&E and CD138 immunostaining for detection of plasma cells. Results: Plasma cells were identified in 52.7% of cases by H&E and in 6/55 by CD138 immunostaining. CD138 is more sensitive in detecting plasma cells in endometrial biopsy than H&E stain. There was a significant statistical correlation between CE and abnormal uterine bleeding, abortion and primary infertility (p > 0.5). Conclusion: Diagnosis of CE is helpful in infertility patients with IVF trial failure to improve the outcome of the maneuver. CD138 is more sensitive for plasma cells specially in endometrial biopsies than H&E.
44
Murphy, L. A., N. Resetkova, K. C. Humm, K. Maas, A. S. Penzias, D. Sakkas, and B. Lannon. "Birthweight outcomes for minimum stimulation in vitro fertilization (MIVF) versus conventional in vitro fertilization (IVF)." Fertility and Sterility 107, no. 3 (March 2017): e29. http://dx.doi.org/10.1016/j.fertnstert.2017.02.053.
Full text
45
Vural, Fisun, Birol Vural, and Yiğit Çakıroğlu. "The Role of Overweight and Obesity in In Vitro Fertilization Outcomes of Poor Ovarian Responders." BioMed Research International 2015 (2015): 1–5. http://dx.doi.org/10.1155/2015/781543.
Full textAbstract:
Objective. Obesity is a worldwide concern with detrimental health effects including decreased fecundity. However, obesity’s impact on in vitro fertilization (IVF) is inconclusive and there is little data concerning poor ovarian responders (POR). This study explored the effects of overweight and obesity on IVF outcomes of POR.Design. We retrospectively evaluated 188 POR undergoing IVF cycles.Methods. Patients were categorized into three groups. Group 1 was normal weight POR (18.5–24.9 kg/m2,n=96); Group 2 was overweight POR (25.0–29.9 kg/m2,n=52); and Group 3 was obese POR (≥30.0 kg/m2,n=40). Main measured outcomes included IVF outcomes.Results. The oocyte maturity, total gonadotropin dose-duration, and cycle cancellation rates were similar. Obese women had significantly decreased LH levels. LH < 4 mIU/mL had a sensitivity (62%) and a specificity (86%) for IVF failure (AUC: 0.71). Fertilization rates of obese subjects were significantly lower than normal and overweight subjects (p=0.04). Obese women’s clinical pregnancy rates were significantly lower (15%) than normal weight women (33.3%,p=0.01).Conclusions. Despite similar counts of recruited mature oocytes, obese POR women had decreased fertilization and clinical pregnancy rates. Obesity rather than overweight significantly decreased IVF outcomes in POR.
46
Tremoleda, J. L., T. A. E. Stout, B. M. Gadella, and B. Colenbrander. "286SPERM-OOCYTE INTERACTION DURING IN VITRO FERTILIZATION IN THE HORSE." Reproduction, Fertility and Development 16, no. 2 (2004): 263. http://dx.doi.org/10.1071/rdv16n1ab286.
Full textAbstract:
In vitro fertilization (IVF) has proven to be a surprisingly unsuccessful way of producing horse embryos. The aim of this study was to investigate the interaction between sperm and the cumulus oocyte complex (COC) during IVF. In experiment 1, three IVF conditions were tested: (A) COCs recovered from slaughtered mares were categorized with respect to cumulus morphology (C: compact, n=86, or E: expanded, n=55) and matured in TCM199 containing 0.01IU/mL porcine FSH and equine LH (IVM); after IVM, the oocytes were denuded and those with a visible polar body were incubated with sperm (IVF) in the presence or absence of 150ng/mL progesterone (P4) to induce the acrosome reaction (AR); (B) IVM oocytes from C-COCS were denuded (n=52) or not (n=67) before IVF in the presence of P4;; (C) in vivo-matured oocytes (n=15) recovered by transvaginal ultrasound-guided aspiration from preovulatory follicles 32h after the donor mare was treated with hCG, were fertilized in vitro in the presence of P4. In all cases, IVF was performed with frozen-thawed, Percoll-selected sperm from a single stallion, at a final concentration of 1×106spermatozoa/ml in fertil-TALP for 20h (Parrish et al., 1988 Biol. Reprod. 38, 1171–1180). In experiment 2, the possibility that semen cryopreservation or stallion critically influenced IVF was examined by incubating denuded IVM oocytes with fresh or frozen/thawed sperm from the same (fresh;; n=17 for both C- and E-COCs and frozen-thawed; n=12 and 21 for C and E-COCs, respectively) or one other stallion (Fresh;; n=12 and 19 and frozen-thawed; n=12 and 19 for C and E-COCs, respectively), in the presence of P4 for 20h. In both experiments, the resulting sperm-oocyte complexes were fixed, permeabilized and labelled with fluorescein-conjugated peanut agglutinin (EY Laboratores, San Mateo, CA, USA) and ethidium homodimer (Molecular Probes, Eugene, OR, USA) to stain the acrosomal membrane and DNA, respectively, so that membrane status and position of the sperm within the oocyte investments could be detected by confocal laser scanning microscopy. The total number of sperm bound per oocyte was compared between treatments using one-way ANOVA with pair-wise multiple comparison (Bonferroni t-test). Despite binding to the zona pellucida (ZP), neither fresh nor frozen/thawed sperm from either stallion acrosome-reacted or penetrated any oocytes, irrespective of cumulus morphology at the onset of IVM, denudation prior to IVF or the presence of P4. However, more sperm bound to the ZP of cumulus-denuded IVM oocytes (65±32 and 62±28 [mean±sd] for C and E-COCs, respectively), than cumulus-intact IVM (5±4) or in vivo-matured oocytes (23±17: P<0.001). None of the other factors investigated affected bound sperm numbers. In all cases, ZP-bound sperm failed to AR in the classical fashion, and all oocytes remained arrested at the MII stage. In summary, fertilization failed because sperm did not acrosome-react after binding to the ZP. It is concluded that failure to adequately activate stallion sperm is an important obstacle to successful IVF in horses.
47
Kakatsaki, D., G. Vaslamatzis, M. Chatziandreou, K. Anastasiadi, U. Dafni, C. Tzavara, K. Stefanidis, D. Loutradis, and A. Antsaklis. "Alexithymia is Positively Associated with the Outcome of in vitro Fertilization (IVF) Treatment." Psychological Reports 105, no. 2 (October 2009): 522–32. http://dx.doi.org/10.2466/pr0.105.2.522-532.
Full textAbstract:
The goal was to examine prospectively the relation of psychological factors to the outcome after in vitro fertilization (IVF) in a sample of Greek women. Previous studies investigating the relation of IVF outcome to anxiety and depression have yielded contradictory results; other psychological dimensions have not been studied. 81 women who followed an IVF program during a 6-mo. period were prospectively evaluated. Demographic and gynecological data were assessed and women completed questionnaires measuring anxiety, depression, narcissistic traits, over-dependency, self-esteem, hostility, and alexithymia. Multiple logistic regression analysis showed that successful IVF outcome correlated with more alexithymic characteristics and younger age. Correlation between alexithymia and successful IVF outcome is demonstrated. This might be explained by the “operational” nature of IVF techniques, to which alexithymic individuals would respond better.
48
Blank, Robert H. "Assisted Reproduction and Reproductive Rights: The Case of In Vitro Fertilization." Politics and the Life Sciences 16, no. 2 (September 1997): 279–88. http://dx.doi.org/10.1017/s0730938400024849.
Full textAbstract:
In vitro fertilization (IVF) and other assisted reproduction technologies (ARTs) have become widely accepted as therapy for a wide array of fertility problems and accompanied by the rapid expansion of clinics that provide a full range of ARTs. Although these technologies undoubtedly offer benefits for some individuals, they raise important questions over reproductive rights to safe and effective treatment as well as access. This article analyzes current data concerning the safety, effectiveness, and cost of IVF. It concludes that IVF and related techniques have been transformed too rapidly and easily from experimental to therapy status, despite evidence that suggests considerable caution is warranted. Unfortunately, the widespread diffusion of IVF has preceded rather than followed firm evidence of its value in extending the reproductive rights of women and couples. Resources might better be directed toward prevention of fertility problems and discovering the causes of infertility.
49
Sinaga, Dina Elizabeth, and Marlina Setiawati Mahajudin. "Couple Therapy pada Pasangan Infertil yang Melakukan In Vitro Fertilization (IVF)." Jurnal Psikiatri Surabaya 6, no. 1 (June 8, 2017): 12. http://dx.doi.org/10.20473/jps.v6i1.19105.
Full textAbstract:
Infertilitas adalah suatu kondisi dimana perempuan dan laki-laki berhubungan seksual secara rutin minimal 1 tahun atau lebih tanpa menjadi hamil.Kondisi ini dapat menimbulkan berbagai reaksi emosi yang dapat mempengaruhi hubungan suami istri.Ada beberapa penanganan yang dapat dilakukan, baik secara fisik maupun psikologis.Secara fisik dapat dilakukan In Vitro Fertilization (IVF).Prosedur IVF ternyata menimbulkan beban psikologis pada pasangan, sehingga diperlukan intervensi psikologis, yang salah satunya dengan couple therapy.Couple therapy bertujuan untuk membantu pasangan memperoleh pengertian yang lebih baik tentang diri mereka sendiri dan pasangannya, serta menolong pasangan untuk mengungkapkan reaksi emosi mereka dengan komunikasi yang baik dan penerimaan terhadap kondisi pasangannya.Ada beberapa pendekatan couple therapy yang dapat dilakukan, diantaranya Emotionally Focused Therapy (EFT) dan Solution Focused Therapy (SFT).Diharapkan dengan dilakukannya intervensi couple therapy sebelum, selama dan setelah prosedur IVF ini, dapat meningkatkan keberhasilan kehamilan sekaligus mencegah kekecewaan dan masalah psikologis akibat kegagalan prosedur IVF.
50
Smith, S. L., L. Y. Sung, R. Page, B. Henderson, F. Du, R. E. Everts, T. Nedambale, et al. "6 EXPRESSION PROFILING OF SINGLE BOVINE EMBRYOS REVEALS SIGNIFICANT EFFECTS OF IN VITRO MATURATION, FERTILIZATION AND CULTURE." Reproduction, Fertility and Development 18, no. 2 (2006): 111. http://dx.doi.org/10.1071/rdv18n2ab6.
Full textAbstract:
Cattle and sheep embryos transferred after in vitro production are often afflicted by large offspring syndrome (LOS), which has been correlated with the presence of serum and/or cell co-culture. Previous research indicates that post-fertilization culture affects blastocyst quality and gene expression, and in vitro oocyte maturation and fertilization impact developmental competence. To dissect the effects of in vitro maturation, fertilization, and culture, we compared the expression profiles of single bovine blastocysts generated by: (1) in vitro maturation, fertilization and culture (IVF, n = 15); (2) in vivo maturation, in vivo fertilization, and in vitro culture (IVD, n = 14); and (3) in vivo maturation, fertilization, and development (AI, n = 14). For in vitro culture, the embryos were cultured for 2 days in CR1aa medium with bovine serum albumin (BSA) and then transferred to CR1aa with 10% fetal bovine serum (FBS) with cumulus cells until Day 7, at which time the embryos were vitrified. IVD zygotes were surgically collected from two superovulated Holstein donor cows 24 h post-insemination and cultured in the same system. To conduct expression profiling, total RNA was isolated from individual thawed embryos. The RNA was subjected to three rounds of amplification utilizing a previously adapted and validated T7 linear amplification protocol. Amplified RNA from each embryo and from a standard reference was indirectly labeled with Cy3 or Cy5 by dye swap and hybridized to a custom bovine cDNA microarray containing ~6300 unique genes. After Loess normalization, an ANOVA model (GeneSpring 6.1 and SAS 9.0) was used to identify differentially expressed genes. The P-values were adjusted for multiple comparisons using the false discovery rate approach, and a e2-fold differential criterion was applied. A subset of the differentially expressed genes was verified by real-time RT-PCR. The blastocyst rates for IVF and IVD embryos were 37% and 75%, respectively. There were 305, 365, and 200 genes differentially expressed between the AI and IVD, the IVF and IVD, and the AI and IVF comparisons, respectively. Interestingly, 44 differentially expressed genes were identified between the AI embryos and both the IVF and the IVD embryos, making these potential candidates for LOS. There were 61 genes differentially expressed between the IVF embryos and the AI and IVD embryos. The Gene Ontology categories 'RNA processing' and 'RNA binding' were over-represented among the genes that were down-regulated in the IVF embryos, indicating an effect of in vitro oocyte maturation/fertilization on embryonic gene expression. This work was supported by USDA grants to X.Y., H.A.L., and X.C.T.