Academic literature on the topic 'ITS-RFLP'
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Journal articles on the topic "ITS-RFLP"
Lanoot, Benjamin, Marc Vancanneyt, Bart Hoste, Katrien Vandemeulebroecke, Margo C. Cnockaert, Peter Dawyndt, Zhiheng Liu, Ying Huang, and Jean Swings. "Grouping of streptomycetes using 16S-ITS RFLP fingerprinting." Research in Microbiology 156, no. 5-6 (June 2005): 755–62. http://dx.doi.org/10.1016/j.resmic.2005.01.017.
Full textKOFFI, YAO FULGENCE, CAMELIA DIGUTA, MIREILLE ALLOUE-BORAUD, LOUIS BAN KOFFI, MARCELLIN DJE, EVELINA GHERGHINA, and FLORENTINA MATEI. "PCR-ITS-RFLP identification of pineapple spoilage fungi." Romanian Biotechnological Letters 24, no. 3 (June 20, 2019): 418–24. http://dx.doi.org/10.25083/rbl/24.3/418.424.
Full textMidgley, David J., Susan M. Chambers, and John W. G. Cairney. "Spatial distribution of fungal endophyte genotypes in a Woollsia pungens (Ericaceae) root system." Australian Journal of Botany 50, no. 5 (2002): 559. http://dx.doi.org/10.1071/bt02020.
Full textDuttweiler, K. B., G. Y. Sun, J. C. Batzer, T. C. Harrington, and M. L. Gleason. "An RFLP-Based Technique for Identifying Fungi in the Sooty Blotch and Flyspeck Complex on Apple." Plant Disease 92, no. 5 (May 2008): 794–99. http://dx.doi.org/10.1094/pdis-92-5-0794.
Full textHazlianda, Cut, Kamaliah Muis, and Isma Lubis. "A Comparative Study of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism and Fungal Culture for the Evaluation of Fungal Species in Patients with Tinea Cruris." Open Access Macedonian Journal of Medical Sciences 5, no. 7 (November 21, 2017): 844–47. http://dx.doi.org/10.3889/oamjms.2017.197.
Full textBurgermeister, Wolfgang, Helen Braasch, Kai Metge, Jianfeng Gu, Thomas Schröder, and Elvira Woldt. "ITS-RFLP analysis, an efficient tool for differentiation of Bursaphelenchus species." Nematology 11, no. 5 (2009): 649–68. http://dx.doi.org/10.1163/156854108/399182.
Full textWang, Qin, and Liang-Dong Guo. "Ectomycorrhizal community composition of Pinus tabulaeformis assessed by ITS-RFLP and ITS sequences." Botany 88, no. 6 (June 2010): 590–95. http://dx.doi.org/10.1139/b10-023.
Full textWurzburger, Nina, Martin I. Bidartondo, and Caroline S. Bledsoe. "Characterization of Pinus ectomycorrhizas from mixed conifer and pygmy forests using morphotyping and molecular methods." Canadian Journal of Botany 79, no. 10 (October 1, 2001): 1211–16. http://dx.doi.org/10.1139/b01-079.
Full textDiao, Ying, Xian-Ming Lin, Chao-Lin Liao, Chun-Zi Tang, Zhong-Jian Chen, and Zhong-Li Hu. "Authentication ofPanax ginsengfrom its Adulterants by PCR-RFLP and ARMS." Planta Medica 75, no. 05 (February 2, 2009): 557–60. http://dx.doi.org/10.1055/s-0029-1185321.
Full textViaud, Muriel, Aymeric Pasquier, and Yves Brygoo. "Diversity of soil fungi studied by PCR-RFLP of ITS." Mycological Research 104, no. 9 (September 2000): 1027–32. http://dx.doi.org/10.1017/s0953756200002835.
Full textDissertations / Theses on the topic "ITS-RFLP"
Anderson, Ian C., of Western Sydney Nepean University, of Science Engineering and Technology Faculty, and School of Science. "Inter- and intraspecific variation in Pisolithus from central and eastern mainland Australia." THESIS_FST_SS_Anderson_I.xml, 2000. http://handle.uws.edu.au:8081/1959.7/237.
Full textDoctor of Philosophy (PhD)
Diguta, Filofteia Camelia. "Ecologie des moisissures présentes sur les baies de raisin." Phd thesis, Université de Bourgogne, 2010. http://tel.archives-ouvertes.fr/tel-00597399.
Full textKeriuscia, Gokul Jarishma. "Eukaryotic diversity of miers valley hypoliths." Thesis, University of the Western Cape, 2012. http://hdl.handle.net/11394/4031.
Full textThe extreme conditions of Antarctic desert soils render this environment selective towards a diverse range of psychrotrophic microbial communities. Cracks and fissures in translucent quartz rocks permit an adequate amount of penetrating light, sufficient water and nutrients to support cryptic microbial development. Hypolithons colonizing the ventral surface of these quartz rocks have been classified into three types: cyanobacterial dominated (Type I),moss dominated (Type II) and lichenized (Type III) communities. Eukaryotic microbial communities were reported to represent only a minor fraction of Antarctic communities. In this study, culture independent techniques (DGGE, T-RFLP and clone library construction) were employed to determine the profile of the dominant eukaryotes, fungi and microalgae present in the three different hypolithic communities. The 18S rRNA gene (Euk for eukaryotes), internal transcribed spacer (ITS for fungi) and microalgal specific regions of the 18S rRNA gene, were the phylogenetic markers targeted for PCR amplification from hypolith metagenomic DNA. Results suggest that the three hypolith types are characterized by different eukaryotic, fungal and microalgal communities, as implied by nMDS analysis of the DGGE and T-RFLP profiles. Sequence analysis indicates close affiliation to members of Amoebozoa, Alveolata, Rhizaria (general eukaryote), Ascomycota (fungal) and Streptophyta (microalgal). Many of these clones may represent novel species. This study demonstrates that Dry Valley hypolithons harbour higher eukaryote diversity than previously recognised.Each hypolithon is colonized by specialized microbial communities with possible keystone species. The ecological role of the detected microorganisms in the hypolith environment is also theorized, and a trophic hierarchy postulated.
Diguta, Camelia Filofteia. "Ecologie des moisissures présentes sur les baies de raisin." Thesis, Dijon, 2010. http://www.theses.fr/2010DIJOS045/document.
Full textMicrobial population of grapes is important from a technological point of view because it determines the quality of wine. But few studies have focused on fungal populations of grapes. A better knowledge of the fungal diversity on grapes, particularly as concerns species responsible for wine defects, may help efforts to control their development.We report the development of a PCR ITS-RFLP method as a fast and easy technique for identifying species of fungal genera present on grapes. By this methode, 41 different fungal species among 43 studied species belonging to 11 genera were characterized at the species level. Only P. thomii remained indistinguishable from P. glabrum. Using this PCR-ITS-RFLP, 96.3% strains tested could be differentiated to the species level with only four enzymes and 41.5% only with two enzymes. Moreover this work has contributed to the enriching of the database of fungal ITS sequences.Thus 199 isolated strain were on grapes in Burgundy vineyard were chacacterized at species level indepdantly of the genus by this method. P. spinolusum was the most frequently isolated species of Penicillium in Burgundy for 2008 vintage. Paralelly, the quantification of Botrytis cinerea, used as model, was developped by qPCR. The assay contained an internal amplification control to compensate for variations in DNA extraction and the various compounds from grapes, had high efficiency and the limit of detection was estimated to be 6.3 pg DNA (corresponding to 540 spores). This method was applied to assess the effects of various treatment strategies against Botrytis in the vineyard and demonstrates the importance of the prophylactic method
Saraiva, Greice Kelle Viegas. "Diversidade genética de leveduras isoladas indústria de leite da Zona da Mata Mineira por RAPD e PCR-RFLP da região ITS do rDNA." Universidade Federal de Viçosa, 2002. http://www.locus.ufv.br/handle/123456789/10643.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
A diversidade genética de vinte e sete isolados de leveduras coletadas em laticínios, utilizando como referências dos gêneros Kluyveromyces e Debaryomyces, foi averiguada por meio de RAPD (Randomly Amplified Polymorphic DNA). A amplificação resultou em um total de oitenta e oito fragmentos polimórfico de DNA, utilizando treze oligonucleotídeos decâmeros aleatórios. As distâncias genéticas variaram de 6,5 a 71%, gerando na análise gráfica, cinco grupos geneticamente divergentes. Nas avaliações da região ITS do rDNA foi observado um polimorfismo de tamanho que variou de 380 a 710 pb. A análise de agrupamento utilizando valores da distância genética resultou na formação de oito grupos, sugerindo a existência de pelo menos oito espécies de leveduras. Na análise por PCR-RFLP da região ITS do rDNA, os produtos das amplificações foram hidrolisados com diferentes endonucleases de restrição evidenciando o padrão polimórfico, e os valores das distâncias genéticas foram utilizados para o agrupamento, resultando na formação de quinze grupos. Os agrupamentos obtidos com os marcadores moleculares possibilitaram a diferenciação genética dos isolados. Os resultados sugerem que quatro dos vinte e sete isolados pertencem à espécie Kluyveromyces lactis.
The genetic diversity of twenty-seven yeasts collected at dairies, was evaluated by RAPD using Kluyveromyces and Debaryomyces reference genera. Amplification using thirteen random oligonucleotides resulted in eighty-eight DNA polymorphic fragments. The Genetic distances varied from 6,5 to 71%, and generated a Dendrogram with five different genetic groups. The amplification of the ITS 18SrDNA region from the yeast resulted in DNA fragments with length polymorphism (380 and 710 bp). The clustering analysis using the genetic distance value produced eight groups, reflecting at least eight yeasts species. The amplification products of the rDNA ITS region using PCR-RFLP analyses were cleaved with different restriction endonucleases showing polymorphic patterns. The values of the genetic distances were used for the clustering resulted in fifteen groups. The clusters obtained using the molecular markers showed genetic difference between you isolates. The results suggest that four of the twenty-seven isolates can be identified as the yeast Kluyveromyces lactis.
Guérin, Alexis. "Les Lactaires à lait rouge : mycorhization controlée des pins et caractérisation moléculaire : Application à l'étude de la compétence écologique et de la compétitivité d'isolats de lactarius deliciosus." Montpellier, ENSA, 1998. http://www.theses.fr/1998ENSA0004.
Full textAnderson, Ian C. "Inter- and intraspecific variation in Pisolithus from central and eastern mainland Australia." Thesis, View thesis, 2000. http://hdl.handle.net/1959.7/uws:237.
Full textMarques, Cálita Pollyanna. "Padronização da análise dos produtos da PCR/RFLP que amplifica os genes Ribossomal Internal transcribed spacer (ITS) e Glucose-6- phosphate dehydrogenase (G6PD) para identificação de Leishmania spp. em gel de poliacrilamida." Universidade Federal de Goiás, 2017. http://repositorio.bc.ufg.br/tede/handle/tede/7789.
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Fundação de Amparo à Pesquisa do Estado de Goiás - FAPEG
American Cutaneous Leishmaniasis (LTA) is caused by protozoa of the genus Leishmania and it attacks the skin and/or mucous membranes. It is an endemic zoonosis in Brazil being notified in all the Regions. There is a relation between the species of parasite and the clinical manifestations, contributing to the diagnostic complexity, which up to the present, did not exist species-specific diagnosis. In the present work, the proposal was to standardize the analysis of the PCR/RFLP products to the ITS and G6PD genes in polyacrylamide gel. In order to standardize PCR/RFLP analysis on the ITS and G6PD genes, DNA from the reference Leishmania spp. Sample was used and 5%, 6%, 7% 8% and 10% polyacrylamide were tested. The protocol design considered the size of the amplicons generated by the PCR / RFLP, correlating the concentration of the polyacrylamide. To standardize the electrophoresis time the migration of the dyes used in the DNA sample buffer was monitored. To validate the technique, we selected 521 samples from Tocantins patients fixed on slides and 43 samples of parasite isolates stored in Leishbank. The samples from the patients from Tocantins were analyzed by PCR/RFLP to the kDNA and were selected to those positive for the validation. The concentration of the matrix for analysis of the PCR and RFLP products to the ITS gene was standardized in 6% and 8%, respectively, and distinguished the species L. (L.) amazonensis, but the species L. (V.) braziliensis, L. (V.) guyanensis and L. (V.) lainsoni were not distinguished among themselves, occurring the same between L. (V.) naiffi and L. (V.) shawi. The concentration of the matrix for analysis of the PCR products to the G6PD gene was standardized in 6% and distinguished L. (V.) braziliensis from the others. The validation of the polyacrylamide gel analysis of the PCR products to the ITS target from samples of patients fixed in slides was not performed, since of the 256 samples analyzed only 4 amplified. The validation of the analysis of the products of the PCR to the G6PD target in polyacrylamide gel from 48 samples fixed in slides characterized 81% as L. (V.) braziliensis and, characterized 63% of the isolates of parasites as L. (V.) braziliensis. The PCR/RFLP analyzes performed to discriminate Leishmania spp. are complementary and thus contribute to the species-specific diagnosis of LTA.
A Leishmaniose Tegumentar Americana (LTA) é causada por protozoários do gênero Leishmania e acomete pele e/ou mucosas. É uma zoonose endêmica no Brasil sendo notificada em todas as Regiões. Há uma relação entre a espécie de parasito e as manifestações clínicas, contribuindo à complexidade diagnóstica, que até o presente, não existi o diagnóstico espécie-específico. No presente trabalho, a proposta foi padronizar a análise dos produtos da PCR/RFLP aos genes ITS e G6PD em gel de poliacrilamida. Para padronizar as análises dos produtos da PCR/RFLP aos genes ITS e G6PD, foram utilizadas DNA da amostra de Leishmania spp., de referência, sendo testada a poliacrilamida na concentração de 5%, 6%, 7% 8% e 10%. O delineamento do protocolo considerou o tamanho dos amplicons gerados pela PCR/RFLP, correlacionando-o a concentração da poliacrilamida. Para padronizar o tempo da eletroforese foi monitorada a migração dos corantes usados no tampão da amostra de DNA. Para validar a técnica, foram selecionadas 521 amostras de pacientes tocantinenses fixadas em lâminas e 43 amostras de isolados de parasitos armazenados no Leishbank. As amostras dos pacientes tocantinenses foram analisadas pela PCR/RFLP ao kDNA e foram selecionadas àquelas positivas para a validação. A concentração da matriz para analise dos produtos da PCR e RFLP ao gene ITS ficou padronizada respectivamente em 6% e 8% e distinguiu a espécie L.(L.) amazonensis, porém as espécies L. (V.) braziliensis, L. (V.) guyanensis e L. (V.) lainsoni não foram distinguidas entre si, ocorrendo o mesmo entre L. (V.) naiffi e L. (V.) shawi. A concentração da matriz para analise dos produtos da PCR ao gene G6PD ficou padronizada em 6% e distinguiu L. (V.) braziliensis das demais. A validação da análise em gel de poliacrilamida dos produtos da PCR ao alvo ITS a partir de amostras de pacientes fixadas em lâminas não ser realizada, pois das 256 amostras analisadas somente 4 amplificaram. A validação da análise dos produtos da PCR ao alvo G6PD em gel de poliacrilamida a partir de 48 amostras fixadas em lâminas caracterizou 81% como L. (V.) braziliensis e, caracterizou 63% dos isolados de parasitos como L. (V.) braziliensis. As análises pela PCR/RFLP realizadas para discriminar Leishmania spp. se complementam e, assim, contribuem para o diagnóstico espécie-específico da LTA.
Yamoah, Emmanuel. "A model system using insects to vector Fusarium tumidum for biological control of gorse (Ulex europaeus)." Phd thesis, Lincoln University. Bio-Protection and Ecology Division, 2007. http://theses.lincoln.ac.nz/public/adt-NZLIU20080131.114607/.
Full textKolenda, Magdalena. "The polymorphism in GDF8 gene and its association with meat performance in the chosen sheep breeds." Rozprawa doktorska, Uniwersytet Technologiczno-Przyrodniczy w Bydgoszczy, 2016. http://dlibra.utp.edu.pl/Content/937.
Full textBooks on the topic "ITS-RFLP"
Hering, Olaf. Charakterisierung und Differenzierung bei Fusarium Link mittels RAPD und ITS-RFLP. Berlin: Parey, 1997.
Find full textBook chapters on the topic "ITS-RFLP"
Rousseaux, Sandrine, and Michèle Guilloux-Bénatier. "PCR ITS-RFLP for Penicillium Species and Other Genera." In Methods in Molecular Biology, 321–33. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6707-0_21.
Full textCroft, J. H., V. Bhattacherjee, and K. E. Chapman. "RFLP Analysis of Nuclear and Mitochondrial DNA and its Use in Aspergillus Systematics." In Modern Concepts in Penicillium and Aspergillus Classification, 309–20. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4899-3579-3_28.
Full textMaccaferri, Marco, Martina Bruschi, and Roberto Tuberosa. "Sequence-Based Marker Assisted Selection in Wheat." In Wheat Improvement, 513–38. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-90673-3_28.
Full textKrause, D., R. Szibor, W. Kuchheuser, and R. Brückner. "The Practical Significance of Human Genetic RFLP-Systems in Paternity Testing." In DNA — Technology and Its Forensic Application, 153–56. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-76632-9_20.
Full textScheithauer, R., and H. J. Weisser. "DNA Extraction and RFLP Analysis of Bloodstains on a Variety of Textiles — Investigation of Various Extraction Procedures." In DNA — Technology and Its Forensic Application, 181–86. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-76632-9_26.
Full text"Appendix IV. Protocols For Identification Of Cyst Nematode Species With PCR-ITS-RFLP Using AB28 And TW81 Primers." In Systematics of Cyst Nematodes (Nematoda: Heteroderinae), Part A, 299–301. BRILL, 2010. http://dx.doi.org/10.1163/ej.9789004162259.i-352.72.
Full textSana, Sadia, Naheed Akhter, Fozia Amjum, Samreen Gul Khan, and Muhammad Akram. "Genetic Diversity in Almond (Prunus dulcis)." In Prunus - Recent Advances [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.99249.
Full textBudakva, Ye, K. Pochernyaev, S. Korinnyi, and M. Povod. "The use of mitochondrial genome polymorphism to establish pro-maternal breeds in the final hybrids of pigs." In Technological innovation engineering manufacturing agricultural complex and zoology (1st ed ), 34–41. Primedia eLaunch LLC, 2020. http://dx.doi.org/10.36074/ti:emacaz.ed-1.03.
Full textConference papers on the topic "ITS-RFLP"
Sharma, Vineeta, Pallavi Singhal, Anoop Kumar, V. G. Ramachandran, Shukla Das, and Mausumi Bharadwaj. "Association of TNF-α–rs 281865419 polymorphism with reproductive tract infections in Indian population." In 16th Annual International Conference RGCON. Thieme Medical and Scientific Publishers Private Ltd., 2016. http://dx.doi.org/10.1055/s-0039-1685357.
Full textSharma, Vineeta, Pallavi Singhal, Anoop Kumar, V. G. Ramachandran, Shukla Das, and Mausumi Bharadwaj. "Association of TNF-α rs-281865419 polymorphism with reproductive tract infections in Indian population." In 16th Annual International Conference RGCON. Thieme Medical and Scientific Publishers Private Ltd., 2016. http://dx.doi.org/10.1055/s-0039-1685270.
Full textReports on the topic "ITS-RFLP"
Dubcovsky, Jorge, Tzion Fahima, and Ann Blechl. Positional cloning of a gene responsible for high grain protein content in tetraploid wheat. United States Department of Agriculture, September 2003. http://dx.doi.org/10.32747/2003.7695875.bard.
Full textFeldman, Moshe, Eitan Millet, Calvin O. Qualset, and Patrick E. McGuire. Mapping and Tagging by DNA Markers of Wild Emmer Alleles that Improve Quantitative Traits in Common Wheat. United States Department of Agriculture, February 2001. http://dx.doi.org/10.32747/2001.7573081.bard.
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