Relevant bibliographies by topics / IPSC derivation

Academic literature on the topic 'IPSC derivation'

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Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "IPSC derivation"

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Tan, Yu-Ting, Lin Ye, Fei Xie, Ashley I. Beyer, Marcus O. Muench, Jiaming Wang, Zhu Chen, Han Liu, Sai-Juan Chen, and Yuet Wai Kan. "Respecifying human iPSC-derived blood cells into highly engraftable hematopoietic stem and progenitor cells with a single factor." Proceedings of the National Academy of Sciences 115, no. 9 (January 31, 2018): 2180–85. http://dx.doi.org/10.1073/pnas.1718446115.

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Derivation of human hematopoietic stem cells (HSCs) from induced pluripotent stem cells (iPSCs) offers considerable promise for cell therapy, disease modeling, and drug screening. However, efficient derivation of functional iPSC-derived HSCs with in vivo engraftability and multilineage potential remains challenging. Here, we demonstrate a tractable approach for respecifying iPSC-derived blood cells into highly engraftable hematopoietic stem and progenitor cells (HSPCs) through transient expression of a single transcription factor, MLL-AF4. These induced HSPCs (iHSPCs) derived from iPSCs are able to fully reconstitute the human hematopoietic system in the recipient mice without myeloid bias. iHSPCs are long-term engraftable, but they are also prone to leukemic transformation during the long-term engraftment period. On the contrary, primary HSPCs with the same induction sustain the long-term engraftment without leukemic transformation. These findings demonstrate the feasibility of activating the HSC network in human iPSC-derived blood cells through expression of a single factor and suggest iHSPCs are more genomically instable than primary HSPCs, which merits further attention.
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Miki, Toshio, Ludivina Vazquez, Lisa Yanuaria, Omar Lopez, Irving M. Garcia, Kazuo Ohashi, and Natalie S. Rodriguez. "Induced Pluripotent Stem Cell Derivation and Ex Vivo Gene Correction Using a Mucopolysaccharidosis Type 1 Disease Mouse Model." Stem Cells International 2019 (April 1, 2019): 1–10. http://dx.doi.org/10.1155/2019/6978303.

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Mucopolysaccharidosis type 1 (MPS-1), also known as Hurler’s disease, is a congenital metabolic disorder caused by a mutation in the alpha-L-iduronidase (IDUA) gene, which results in the loss of lysosomal enzyme function for the degradation of glycosaminoglycans. Here, we demonstrate the proof of concept of ex vivo gene editing therapy using induced pluripotent stem cell (iPSC) and CRISPR/Cas9 technologies with MPS-1 model mouse cell. Disease-affected iPSCs were generated from Idua knockout mouse embryonic fibroblasts, which carry a disrupting neomycin-resistant gene cassette (Neor) in exon VI of the Idua gene. Double guide RNAs were used to remove the Neor sequence, and various lengths of donor templates were used to reconstruct the exon VI sequence. A quantitative PCR-based screening method was used to identify Neor removal. The sequence restoration without any indel mutation was further confirmed by Sanger sequencing. After induced fibroblast differentiation, the gene-corrected iPSC-derived fibroblasts demonstrated Idua function equivalent to the wild-type iPSC-derived fibroblasts. The Idua-deficient cells were competent to be reprogrammed to iPSCs, and pluripotency was maintained through CRISPR/CAS9-mediated gene correction. These results support the concept of ex vivo gene editing therapy using iPSC and CRISPR/Cas9 technologies for MPS-1 patients.
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Lee, Hyelim, Hyeonjin Cha, and Ju Hyun Park. "Derivation of Cell-Engineered Nanovesicles from Human Induced Pluripotent Stem Cells and Their Protective Effect on the Senescence of Dermal Fibroblasts." International Journal of Molecular Sciences 21, no. 1 (January 5, 2020): 343. http://dx.doi.org/10.3390/ijms21010343.

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Stem cells secrete numerous paracrine factors, such as cytokines, growth factors, and extracellular vesicles. As a kind of extracellular vesicle (EV), exosomes produced in the endosomal compartment of eukaryotic cells have recently emerged as a biomedical material for regenerative medicine, because they contain many valuable contents that are derived from the host cells, and can stably deliver those contents to other recipient cells. Although we have previously demonstrated the beneficial effects of human induced potent stem cell-derived exosomes (iPSC-Exo) on the aging of skin fibroblasts, low production yield has remained an obstacle for clinical applications. In this study, we generated cell-engineered nanovesicles (CENVs) by serial extrusion of human iPSCs through membrane filters with diminishing pore sizes, and explored whether the iPSC-CENV ameliorates physiological alterations of human dermal fibroblasts (HDFs) that occur by natural senescence. The iPSC-CENV exhibited similar characteristics to the iPSC-Exo, while the production yield was drastically increased compared to that of iPSC-derived EVs, including exosomes. The proliferation and migration of both young and senescent HDFs were stimulated by the treatment with iPSC-CENVs. In addition, it was revealed that the iPSC-CNEV restored senescence-related alterations of gene expression. Treatment with iPSC-CENVs significantly reduced the activity of senescence-associated-β-galactosidase (SA-β-Gal) in senescent HDFs, as well as suppressing the elevated expression of p53 and p21, key factors involved in cell cycle arrest, apoptosis, and cellular senescence signaling pathways. Taken together, these results suggest that iPSC-CENV could provide an excellent alternative to iPSC-exo, and be exploited as a resource for the treatment of signs of skin aging.
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Yu, Yang, Xuechun Li, Yimei Li, Renyue Wei, Hai Li, Zhonghua Liu, and Yu Zhang. "Derivation and Characterization of Endothelial Cells from Porcine Induced Pluripotent Stem Cells." International Journal of Molecular Sciences 23, no. 13 (June 24, 2022): 7029. http://dx.doi.org/10.3390/ijms23137029.

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Although the study on the regulatory mechanism of endothelial differentiation from the perspective of development provides references for endothelial cell (EC) derivation from pluripotent stem cells, incomplete reprogramming and donor-specific epigenetic memory are still thought to be the obstacles of iPSCs for clinical application. Thus, it is necessary to establish a stable iPSC-EC induction system and investigate the regulatory mechanism of endothelial differentiation. Based on a single-layer culture system, we successfully obtained ECs from porcine iPSCs (piPSCs). In vitro, the derived piPSC-ECs formed microvessel-like structures along 3D gelatin scaffolds. Under pathological conditions, the piPSC-ECs functioned on hindlimb ischemia repair by promoting blood vessel formation. To elucidate the molecular events essential for endothelial differentiation in our model, genome-wide transcriptional profile analysis was conducted, and we found that during piPSC-EC derivation, the synthesis and secretion level of TGF-β as well as the phosphorylation level of Smad2/3 changed dynamically. TGF-β-Smad2/3 signaling activation promoted mesoderm formation and prevented endothelial differentiation. Understanding the regulatory mechanism of iPSC-EC derivation not only paves the way for further optimization, but also provides reference for establishing a cardiovascular drug screening platform and revealing the molecular mechanism of endothelial dysfunction.
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Assen, Lars, Annelien Bredenoord, Karin Jongsma, Marianna Tryfonidou, and Rosario Isasi. "iPS Cells: Don’t Forget about the Soft Impacts." Studia Universitatis Babeş-Bolyai Bioethica 66, Special Issue (September 9, 2021): 26–27. http://dx.doi.org/10.24193/subbbioethica.2021.spiss.08.

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"Induced pluripotent stem cells (iPSCs) have been praised for overcoming some of the ethical challenges of embryonic stem cell research, including oocyte donation for research and the destruction of human embryos. However, iPSC-research and iPSC-based interventions are not morally neutral alternatives and have their own ethical implications that are not fully understood yet. While there is some understanding of ethical issues surrounding the derivation, storage and use of human tissue, there is less understanding of how iPSC-research affects our society and morality. Consequentially, it is difficult to fully anticipate those implications. The notion of hard and soft impacts could benefit the understanding and anticipation of ethical implications of iPSC-research and interventions. Hard impacts are those direct physical and financial effects of iPSCs that are quantifiable and measurable. So-called soft impacts have a different focus. They consider how a technology or intervention affects our psychology, societal structures, morality and our behavior, hereby influencing the uptake, effects and evaluation of technology. So far, academic literature and researchers focus primarily on hard impacts of iPSC-research. Soft impacts are similarly important and therefore require more academic and regulatory attention. This talk focuses upon these understudied aspects of iPSC-research and technology. The goal is to show that for researchers and ethicists it is important to become aware of the soft impacts of iPSC-research and technology. This awareness could contribute to a broader understanding of the social value of stem cell research, anticipating ethical challenges of iPSC-research and in formulating new virtues for stem cell researchers. "
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Pinto, A., Y. Shamis, L. McDonnell, A. Chambon, M. Jung, J. Valls Cuevas, R. Chaffoo, M. Samberg, C. Sumen, and A. Terskikh. "Derivation of folliculogenic organoids from human iPSC." Cytotherapy 23, no. 5 (May 2021): S17. http://dx.doi.org/10.1016/s1465324921002711.

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Srimasorn, Sumitra, Matthias Kirsch, Susanne Hallmeyer-Ellgner, Dirk Lindemann, Alexander Storch, and Andreas Hermann. "Increased Neuronal Differentiation Efficiency in High Cell Density-Derived Induced Pluripotent Stem Cells." Stem Cells International 2019 (December 4, 2019): 1–8. http://dx.doi.org/10.1155/2019/2018784.

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Human pluripotent stem cells (hPSCs), including induced pluripotent stem cells (iPSCs), provide access to hard-to-obtain cells for studies under physiological and disease conditions. For the study of neurodegenerative diseases, especially sporadic cases where the “disease condition” might be restricted towards the neuroectodermal lineage, obtaining the affected neurons is important to help unravel the underlying molecular mechanism leading to the diseases. Although differentiation of iPSCs to neural lineage allows acquisition of cell types of interest, the technology suffers from low efficiency leading to low yield of neurons. Here, we investigated the potential of adult neuroprogenitor cells (aNPCs) for iPSC derivation and possible confounders such as cell density of infected NPCs on their subsequent neuronal differentiation potential from reprogrammed cells under isogenic conditions. Characterized hiPSCs of defined cell densities generated from aNPCs were subjected to neuronal differentiation on PA6 stromal cells. The results showed that hiPSC clones obtained from low seeding density (iPSC-aNPCLow) differentiated less efficiently compared to those from higher density (iPSC-aNPCHigh). Our findings might help to further improve the yield and quality of neurons for in vitro modelling of neurodegenerative diseases.
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Petkov, Stoyan, Ralf Dressel, Ignacio Rodriguez-Polo, and Rüdiger Behr. "Controlling the Switch from Neurogenesis to Pluripotency during Marmoset Monkey Somatic Cell Reprogramming with Self-Replicating mRNAs and Small Molecules." Cells 9, no. 11 (November 5, 2020): 2422. http://dx.doi.org/10.3390/cells9112422.

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Induced pluripotent stem cells (iPSCs) hold enormous potential for the development of cell-based therapies; however, the safety and efficacy of potential iPSC-based treatments need to be verified in relevant animal disease models before their application in the clinic. Here, we report the derivation of iPSCs from common marmoset monkeys (Callithrix jacchus) using self-replicating mRNA vectors based on the Venezuelan equine encephalitis virus (VEE-mRNAs). By transfection of marmoset fibroblasts with VEE-mRNAs carrying the human OCT4, KLF4, SOX2, and c-MYC and culture in the presence of small molecule inhibitors CHIR99021 and SB431542, we first established intermediate primary colonies with neural progenitor-like properties. In the second reprogramming step, we converted these colonies into transgene-free pluripotent stem cells by further culturing them with customized marmoset iPSC medium in feeder-free conditions. Our experiments revealed a novel paradigm for flexible reprogramming of somatic cells, where primary colonies obtained by a single VEE-mRNA transfection can be directed either toward the neural lineage or further reprogrammed to pluripotency. These results (1) will further enhance the role of the common marmoset as animal disease model for preclinical testing of iPSC-based therapies and (2) establish an in vitro system to experimentally address developmental signal transduction pathways in primates.
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Pouyanfard, Somayeh, Manuel Fierro, and Dan S. Kaufman. "Development of Chimeric Antigen Receptor-Expressing iPSC-Derived Macrophages with Improved Anti-Tumor Activity." Blood 138, Supplement 1 (November 5, 2021): 1693. http://dx.doi.org/10.1182/blood-2021-148687.

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Abstract Previous studies by our group demonstrate the ability to routinely derive hematopoietic and immune cells from human pluripotent stem cells. Here, we demonstrate the efficient derivation of macrophages from human induced pluripotent stem cells (iPSCs). These macrophages have phenotypic and genotypic characteristics similar to monocytes/macrophages isolated from human peripheral blood. We also demonstrate the ability to polarize these iPSC-derived macrophages (iPSC-Macs) to M1 and M2 populations. Specifically, M1 iPSC-Macs have pro-inflammatory characteristics including expression of CD40 and CD80 on the cell surface, produce increased amounts of TNF-a and IL-6 detected in the supernatant, as well have increased expression of inflammatory cytokines/chemokines (TNF-a, IL-6, IL-1b, IL-12, CCL2, CCL3 and TRAIL) and increased expression of matrix metalloproteases (MMPs). Function of these iPSC-Macs was initially assessed by phagocytosis of fluorescently-labeled beads. These studies demonstrated both the iPSC-M1 and M2 macrophages efficiently phagocytized these beads, and at similar amounts as their peripheral blood counterparts. Next, we tested the ability of the iPSC-Macs to phagocytize human tumor cells. Using A1847 ovarian tumor cells, we found while the iPSC-Macs alone had limited ability to phagocytize the tumor cells (9%), addition of either an anti-CD47 mAb (41%) or anti-EGFR (41%) lead to markedly increased phagocytosis, with the combination of the 2 antibodies being even better (55% phagocytosis). We then tested iPSC-Macs in vivo against luciferase (luc)-expressing A1847 ovarian cancer cells as a xenograft model in NSG-SGM3 mice that express human IL3, GM-CSF and SCF. Using bioluminescent imaging, we found that the combination of iPSC-Macs with both anti-CD47 and anti-EGFR demonstrated significantly improved anti-tumor activity, with median survival of 75 days, compared to 50-60 days for mice treated with only iPSC-Macs, only mAbs or with iPSC-Macs combined either single mAb. Next, we aimed to use the iPSC platform to produce iPSC-Macs engineered to express chimeric antigen receptors (CARs) to further improve their anti-tumor activity. Here, we developed and tested novel macrophage specific CARs that were stably expressed in undifferentiated iPSCs using transposon-mediated gene transfer, similar to our previous studies to derive iPSC-derived CAR-expressing NK cells that have now been translated into clinical trials. We used an anti-mesothelin (meso) scFv combined with 8 different CAR constructs with distinct intracellular signaling components. We found that the iPSC-Macs could express good levels of the CARs (iPSC-CarMacs). Function was again tested in vitro by phagocytosis of the Meso+ A1847 ovarian cancer cells. The iPSC-CarMacs with a Bai1 stimulatory domain consistently demonstrated the best activity in this assay system. We next tested the anti-meso-iPSC-CarMacs in vivo using the A1847 cells. Again, we demonstrate the iPSC-CarMacs combined with anti-CD47 mAb mediate significantly improved anti-tumor activity using this in vivo model compared to the non-CAR-iPSC-Macs + anti-CD47, p <0.005 (Figure). Survival studies are still ongoing. Together, these studies demonstrate that iPSCs can be used to routinely and efficiently derive macrophages with potent anti-tumor activity. Additionally, CARs that are optimized for macrophage-mediated activity can be expressed to generate iPSC-CarMacs that effectively kill tumor cells in vitro and in vivo. These iPSC-CarMacs provide another approach to provide a standardized, targeted, off-the-shelf cell therapy product that can be used to treat both hematological malignancies as well as diverse solid tumors. Figure 1 Figure 1. Disclosures Kaufman: Shoreline Biosciences: Consultancy, Current holder of stock options in a privately-held company, Membership on an entity's Board of Directors or advisory committees, Research Funding; Qihan Biotech: Consultancy, Current holder of stock options in a privately-held company; VisiCELL Medical: Consultancy, Current holder of stock options in a privately-held company.
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Schuening, Friedrich, Michail Zaboikin, Tatiana Zaboikina, and Narasimhachar Srinivasakumar. "Derivation of Hematopoietic Progenitor Cells From Vector-Free Human Induced Pluripotent Stem Cells." Blood 120, no. 21 (November 16, 2012): 4746. http://dx.doi.org/10.1182/blood.v120.21.4746.4746.

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Abstract Abstract 4746 Induced pluripotent stem cells (iPSCs), due to their self-renewal and differentiation capability, have tremendous potential in regenerative medicine. Differentiation of IPSCs in vitro to obtain sufficient number of hematopoietic stem cells (HSCs) and their progenitors (HPCs) from iPSCs for therapeutic purposes is a holy grail of cellular therapy. To this end, we are comparing different in vitro differentiation approaches for generation of HSCs/HPCs from IPSCs. We have generated iPSCs from human adult dermal fibroblasts using two different reprogramming methods: 1) Transduction with retroviral vectors encoding human Klf4, Oct3/4, Sox2 and cMyc or 2) Electroporation with Epstein–Barr virus (EBV) based episomal plasmid vectors encoding Klf4, Oct3/4, Sox2, L-Myc and p53 targeting shRNA. The transduced/electroporated cells were reprogrammed on SNL5 mouse feeder cells. Putative iPSC-like colonies were cloned and adapted to grow under feeder-free conditions on Matrigel (BD) in mTeSR1 (Stem Cell Technologies) medium. From over 30 individual clones isolated, six were further characterized for: 1) expression of pluripotency markers (Tra-1–60, SSEA-3, SSEA-4, Nanog and Oct3/4) by immunofluorescence; 2) endogenous and total mRNA expression by quantitative real-time reverse-transcriptase PCR (RT-qPCR) for Klf4, Oct3/4, Sox2 and cMyc to distinguish between cellular and vector derived expression of reprogramming factors; 3) RT-qPCR to determine expression of other markers of pluripotency such as Nanog and DNA methyl transferease; 4) karyotype analysis to determine chromosomal anomalies. The vector-free IPSC clones were also tested for residual integrated EBV plasmid DNA by qPCR. Trilineage differentiation ability of the clones was determined through embryoid body formation in suspension cultures, and subsequent staining of resulting embryoid bodies after adherence to gelatin coated dishes for makers of ectoderm, mesoderm and endoderm. HSCs/HPCs were obtained from IPSCs by 1) coculture with OP9 stromal cells, or 2) step-wise differentiation in feeder-free conditions on Matrigel under defined conditions in the presence of appropriate growth factors [Niwa A et al. PLoS One. (2011); 6(7):e22261.]. The resultant HSCs/HPCs were subjected to colony forming assays in semi-solid medium containing hematopoietic cytokines. Both erythroid and myelomonocytic colonies could be readily identified. The influence of ambient oxygen concentration on the HSC/HPC derivation procedure is being investigated. The results of these studies will be presented. Disclosures: No relevant conflicts of interest to declare.
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Dissertations / Theses on the topic "IPSC derivation"

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Spitzhorn, Lucas-Sebastian [Verfasser], and James [Gutachter] Adjaye. "Derivation, characterization and application of human primary stem cells, iPSCs, and iPSC-derived MSCs for regenerative medicine and disease modelling / Lucas-Sebastian Spitzhorn ; Gutachter: James Adjaye." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2020. http://d-nb.info/1203369867/34.

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Goto, Kazuya. "Simple Derivation of Spinal Motor Neurons from ESCs/iPSCs Using Sendai Virus Vectors." Kyoto University, 2017. http://hdl.handle.net/2433/226763.

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Yang, Chian. "Derivation of purified smooth muscle cells from mouse induced pluripotent stem (iPS) cells." Thesis, Boston University, 2013. https://hdl.handle.net/2144/12250.

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Thesis (M.S.)--Boston University
Cardiac and vascular disease syndromes and abnormalities have long been the leading causes of death in the United States, but the cause of congenital defects remain unclear due to the lack of appropriate model systems for scientific investigation. Moreover, the predominance of cardiovascular disease has stimulated scientists to focus on developing tissue-engineered blood vessels (TEBV) for vascular reconstruction and replacement. Major challenges remain in generating large amounts of epithelial cells (EC) and vascular smooth muscle cells (VSMC) for vascular reconstruction and in reducing the immunoresponse in patients after replacement. To address both issues of disease model generation and vascular tissue engineering, we can use induced pluripotent stem (iPS) cells discovered by Shinya Yamanaka in 2006: iPS cells generated from adult tissue and organs through the forced expression of two to four transcription factors are phenotypically indistinguishable from embryonic stem (ES) cells. First, by creating iPS from cardiovascular patients, we can generate patient-specific cell lines for scientific research investigation (i.e. elucidate molecular mechanisms and potential drug targets). Second, EC and VSMC derived from patient-specific iPS cell lines can be used for vascular reconstruction with cells of the patient's own genetic background, which should overcome many of the immunological limitations that currently impede vascular replacement (i.e. provide therapeutic interventions). The goal of this project is to study the differentiation capacity of iPS cells into smooth muscle cells (SMC). This project aims to develop a protocol that can maximize the derivation of purified smooth muscle cells from mouse induced pluripotent stem (iPS) cells through three linear developmental stages: induction of a posterior primitive- streak (PS) like population, formation of Flk1+ mesoderm, and induction of smooth muscle cells. Low dosage of Activin A and Wnt was used to differentiate iPS into a PS-like population, while the administration of BMP4 differentiates the cells to mesoderm via a posterior PS intermediate. Smooth muscle cells were induced from mesoderm by the addition of platelet-derived growth factor (PDGF-BB) and transforming growth factor b (TGF-β). The linear developmental progression from PS formation through mesoderm induction to smooth muscle cells were tracked by RT-qPCR and FACS for the expression of genes indicative of each individual stage, Flk1, and SMαA respectively. The results of this project can be used as a basis for in vitro derivation of purified mammalian smooth muscle cells from a mouse model system that can be further modified. Moreover, the differentiated SMCs can be further used in cell sheet construction as vascular patches for drug testing.
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Books on the topic "IPSC derivation"

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Nat, Roxana, and Andreas Eigentler. Cell Culture, iPS Cells and Neurodegenerative Diseases. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780190233563.003.0013.

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Somatic reprogramming technology, which enables the conversion of adult human non-neural cells into neurons, has progressed rapidly in recent years. The derivation of patient-specific induced pluripotent stem (iPS) cells has become routine. The inherent broad differentiation potential of iPS cells makes possible the generation of diverse types of human neurons. This constitutes a remarkable step in facilitating the development of more appropriate and comprehensive preclinical human disease models, as well as for high throughput drug screenings and cell therapy. This chapter reviews recent progress in the human iPS cell culture models related to common and rare NDDs, such as Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, amyotrophic lateral sclerosis, spinal muscular atrophy, and degenerative ataxias. It focuses on the pathophysiological features revealed in cell cultures, and the neuronal subtypes most affected in NDDs. The chapter discusses the validity, limitation, and improvements of this system in faithfully and reproducibly recapitulating disease pathology.
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Book chapters on the topic "IPSC derivation"

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Malik, Nasir, and Mahendra S. Rao. "A Review of the Methods for Human iPSC Derivation." In Methods in Molecular Biology, 23–33. Totowa, NJ: Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-348-0_3.

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Liang, Dongli, and Yuan Wang. "Male germ cell derivation from PSCs." In Recent Advances in iPSC-Derived Cell Types, 133–65. Elsevier, 2021. http://dx.doi.org/10.1016/b978-0-12-822230-0.00007-7.

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Meganathan, Kesavan, Ramachandran Prakasam, Komal Kaushik, Irene Antony, Gareth Chapman, and Kristen L. Kroll. "Derivation of cortical interneurons from human pluripotent stem cells to model neurodevelopmental disorders." In Phenotyping of Human iPSC-derived Neurons, 45–72. Elsevier, 2023. http://dx.doi.org/10.1016/b978-0-12-822277-5.00007-9.

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Konala, Vijay Bhaskar Reddy, Swapna Nandakumar, Harshini Surendran, and Rajarshi Pal. "Derivation of Induced Pluripotent Stem Cell (iPSC) Lines from Patient-Specific Peripheral Blood Mononuclear Cells (PBMC) Using Episomal Vectors." In Methods in Molecular Biology. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/7651_2021_385.

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Winder, Matthew L., and Ras Trokovic. "Induced pluripotent stem cell derivation from myoblasts." In Cell Sources for iPSCs, 37–55. Elsevier, 2021. http://dx.doi.org/10.1016/b978-0-12-822135-8.00009-4.

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Jamal, Mohamed, Asma Bashir, Mohamed Al-Sayegh, and George T. J. Huang. "Oral tissues as sources for induced pluripotent stem cell derivation and their applications for neural, craniofacial, and dental tissue regeneration." In Cell Sources for iPSCs, 71–106. Elsevier, 2021. http://dx.doi.org/10.1016/b978-0-12-822135-8.00007-0.

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Zhao, Qingguo, and Fei Liu. "Derivation and Characterization of Mesenchymal Stem Cells from iPS Cells." In Methods in Molecular Biology. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/7651_2020_327.

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Kim, Myung-Hoon. "Advances in Derivative Voltammetry - A Search for Diagnostic Criteria of Several Electrochemical Reaction Mechanisms." In Analytical Chemistry - Advancement, Perspectives and Applications. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.96409.

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New methods for analysis of current-potential curves in terms of their derivatives are presented for studying various types of electrode processes – such as simple electron transfer reactions (reversible, quasi-reversible, and irreversible electron transfer) as well as chemically coupled electron transfer reactions along with a diagnostic scheme for differentiating these various types of electrochemical reaction mechanisms. Expressions for first- and higher order derivatives are derived from theoretical analytical solutions for currents for the different types of electrode mechanisms. The derivative curves are analyzed in terms of various parameters which characterize peak shape or peak symmetry with an emphasis on the second derivatives with well-defined anodic and cathodic peaks. Second derivatives can yield, in a simpler manner, the symmetry ratios; i.e., a ratio of anodic to cathodic peak-currents (ipa/ipc), and a ratio of anodic to cathodic peak-widths (Wpa/wpc) and a ratio of anodic to cathodic peak potential differences (ΔEpa/ΔEpc) or a peak separation (Epa-Epc) are evaluated, and these ratio can be related to kinetic parameters associated with a particular types of electrode mechanisms. Peaks are found to be symmetrical for a simple reversible electron transfer process (Er). However, peaks become asymmetrical when the electron transfer become slower (namely, irreversible, Eirr) or e− transfer reaction is coupled with homogeneous chemical reactions such as a prior reaction (CEr) or a follower-up reaction (ECr). From measured values of such symmetry ratios above, one can gain insight to the nature of the electrochemical systems enabling us to determine various kinetic parameters associated with a system. A diagnostic criteria for assigning an electrode mechanism is devised based on the values of asymmetry parameters measured, which are unity for a simple reversible electron transfer process.
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Fleming, James R. "Introduction: Apprehending Climate Change." In Historical Perspectives on Climate Change. Oxford University Press, 1998. http://dx.doi.org/10.1093/oso/9780195078701.003.0005.

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Apprehensions have been multiplying rapidly that we are approaching a crisis in our relationship with nature, one that could have potentially catastrophic results for the sustainability of civilization and even the habitability of the planet. Much of the concern is rightfully focused on changes in the atmosphere caused by human activities. Only a century after the discovery of the stratosphere, only five decades after the invention of chlorofluorocarbons (CFCs), and only two decades after atmospheric chemists warned of the destructive nature of chlorine and other compounds, we fear that ozone in the stratosphere is being damaged by human activity. Only a century after the first models of the carbon cycle were developed, only three decades after regular CO2 measurements began at Mauna Loa Observatory, and only two decades after climate modelers first doubled the CO2 in a computerized atmosphere, we fear that the Earth may experience a sudden and possibly catastrophic warming caused by industrial pollution. These and other environmental problems were brought to our attention mainly by scientists and engineers, but the problems belong to us all. Recently, policy-oriented social scientists, public officials, and diplomats have turned their attention to the complex human dimensions of these issues. New interdisciplinary and multidisciplinary collaborations have arisen between scientists and policymakers to examine the extremely challenging issues raised by global change. There has been a rising tide of literature—scholarly works, new journals, textbooks, government documents, treaties, popular accounts—some quite innovative, others derivative and somewhat repetitious. This has resulted in growing public awareness of environmental issues, new understanding of global change science and policy, widespread concerns over environmental risks, and recently formulated plans to intervene in the global environment through various forms of social and behavioral engineering, and possibly geoengineering. Global change is now at the center of an international agenda to understand, predict, protect, and possibly control the global environment. The changing nature of global change—the historical dimension—has not received adequate attention. Most writing addresses current issues in either science or policy; much of it draws on a few authoritative scientific statements such as those by the Intergovernmental Panel on Climate Change (IPCC); almost none of it is informed by historical sensibility.
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Conference papers on the topic "IPSC derivation"

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Johare, Dzulfadly B., M. Mehmet Altunbay, KoKoKyi Kyi, and Raymond E. Poit. "Derivation of Residual Oil Profile for Enhanced Recovery." In International Petroleum Technology Conference. International Petroleum Technology Conference, 2013. http://dx.doi.org/10.2523/iptc-16435-ms.

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Cobanoglu, Mustafa. "A New Method of Pressure Analysis of Well-Test Data from a Well in a Multi-well Reservoir with no Flow Boundary Condition & New Diagnostic Plots to Differentiate Between the Boundary and Interference Effects." In International Petroleum Technology Conference. IPTC, 2021. http://dx.doi.org/10.2523/iptc-21373-ms.

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Abstract If the interference effect is not considered for well test interpretation, it could lead to wrong analyses especially in boundary identification. In addition, there are some case where interference effects might be hidden where it may not be obvious due to data uncertainty. Therefore, special diagnostics of the multi well interference models will be required to differentiate between the boundary and interference effects. In addition, there is no analytical method for a well in a multi-well reservoir with no flow boundary condition. In this paper a new method was developed to model Pressure Analysis of Well-Test Data from a Well in a Multi-well reservoir with no flow boundary condition. It covers; Derivation of the analytical model, based on the superposition principle, with and without "no flow" boundary condition; Modeling of various combination of testing & interfering well cases (i.e. testing well is on production or shut-in while interfering well is on production or shut-in) Modeling of various combinations of testing & interfering well rate cases (i.e. production or injection, rate variations) Modeling of various number of interfering well cases (i.e. location and well count) Investigation deeply on how to differentiate between the boundary and interference effects (or vice versa) and developing the special diagnostics to able to detect interference effect directly. Results are shown that 1) the multi-well interference effect with and without no flow boundary condition has huge impact on the well test interpretation and this effect might be interpreted as a boundary effect if interference is not considered. 2) Build-up (BU) behavior of testing well is depending on whether interfering well is shut-in or producing. If interfering well is producing, pressure derivative of BU curve is concave down and If the interfering wells are shutting in, pressure derivative of BU curve is concave up 3) the interfering well rate is affecting magnitude of impact on pressure derivative and the higher the rates, the bigger the response 4) the interfering well distance is affecting the timing of deviation on pressure derivative and the closer the distance, the quicker the response Study also concluded that there are 3 special characteristics, which only exists in interference cases, and which does not exist in boundary cases. Therefore, those characteristics can be used to differentiate between the interference and boundary effects. Those are 1) Pressure decrease or rise at the beginning of well testing 2) the drawn-down (DD) and BU pressure derivatives in Log-Log plot are different (i.e. when BU is concave up, DD is concave down or vice versa) in case of interfering well is on continuous production 3) The consecutive BU's (or DD's) pressure derivatives on Log-Log plot are different and changing over time
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Michopoulos, John G., and Athanasios P. Iliopoulos. "Modeling the Multiphysics Wrinkling Instability of Ionic Polymer Composite Plates for Artificial Muscle Applications." In ASME 2016 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2016. http://dx.doi.org/10.1115/detc2016-59620.

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In this paper we first present the derivation of the governing equations that describe the multiphysics behavior of Ionic Polymer Composite Plates (IPMC). This is done in a manner that accounts for their non-linear large deflection deformation under the influence of mechanical, electrical, thermal and multicomponent mass transport fields. We subsequently present numerical solutions of the system of these equations via the use of the finite element method for a case of a specific rectangular plate. Emphasis is given in identifying the multiphysics based wrinkling instability behavior that manifest near the corners of these plates due to multiphysics stimuli.
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Petersen, Henrik I., Hans P. Nytoft, Michael B. W. Fyhn, Nguyen T. Dau, Ha T. Huong, J. A. Bojesen-Koefoed, and Lars H. Nielsen. "Oil and Condensate Types In Cenozoic Basins Offshore Vietnam: Composition And Derivation." In International Petroleum Technology Conference. International Petroleum Technology Conference, 2011. http://dx.doi.org/10.2523/iptc-14383-ms.

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5

Boughner, Abe. "A New Approach to Gas Turbine Systems in the All Electric Warship Era." In ASME Turbo Expo 2003, collocated with the 2003 International Joint Power Generation Conference. ASMEDC, 2003. http://dx.doi.org/10.1115/gt2003-38666.

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This paper will focus on discussions of new concepts for integration of gas turbines into advanced warship designs. The advancement of the electric warship creates several revolutionary opportunities in ship design concepts. The Integrated Power System (IPS) combines propulsion and auxiliary loads such that any electric generator can supply any load including electric propulsion, combat and hotel loads with light areo derivative gas turbines serving as prime movers for power generation. A combination of small and large gas turbine generators are fitted so that the operator can match the on-line generation capacity to the demand thereby keeping the gas turbines loaded to efficient levels. The IPS, or All Electric Warship concept, although a matter of much interest and study, will not be discussed in this paper. This paper will discuss how the light aero engine coupled with the IPS concept allows greater flexibility in overall design of the machinery plant as well as several new concepts for gas turbine systems in warships.
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Wang, Hao, Jinjun Liu, and Dan Hou. "Derivation method of output impedance of DC-DC converters paralleled system with active current sharing control for system stability analysis." In 2010 International Power Electronics Conference (IPEC - Sapporo). IEEE, 2010. http://dx.doi.org/10.1109/ipec.2010.5543863.

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Ling, Kegang, and Jun He. "Equation for Oil and Gas Two-Phase Flow into Vertical Well - A Theoretical Derivation." In International Petroleum Technology Conference. International Petroleum Technology Conference, 2013. http://dx.doi.org/10.2523/iptc-16627-ms.

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Forin, F., K. J. Czogalla, H. Singer, M. Watzka, and J. Oldenburg. "Derivation of Patient Specific iPSCs and iHEP Cell Lines as a Tool to Investigate VKCFD2 in a Native Cellular Environment." In 63rd Annual Meeting of the Society of Thrombosis and Haemostasis Research. Georg Thieme Verlag KG, 2019. http://dx.doi.org/10.1055/s-0039-1680266.

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Malekizandi, M., Q. T. Le, A. Emsia, and F. Kuppers. "Generating 6thorder Gaussian derivative for UWBoF using directly modulated laser and accumulative chromatic dispersion of fiber." In 2015 IEEE Photonics Conference (IPC). IEEE, 2015. http://dx.doi.org/10.1109/ipcon.2015.7323638.

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Harris, J. "Derivation of Relative Permeability & Fractional Flow Behaviour from the Inversion of Saturation Logs in Horizontal Wells with Application to Water Shut-off and Predicting Volumetric Sweep Efficiency." In IPTC 2007: International Petroleum Technology Conference. European Association of Geoscientists & Engineers, 2007. http://dx.doi.org/10.3997/2214-4609-pdb.147.iptc11467.

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