Academic literature on the topic 'Intravital microscopy techniques'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Intravital microscopy techniques.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Journal articles on the topic "Intravital microscopy techniques"
Choi, Myunghwan, Sheldon J. J. Kwok, and Seok Hyun Yun. "In Vivo Fluorescence Microscopy: Lessons From Observing Cell Behavior in Their Native Environment." Physiology 30, no. 1 (January 2015): 40–49. http://dx.doi.org/10.1152/physiol.00019.2014.
Full textNorman, Keith. "Techniques: Intravital microscopy – a method for investigating disseminated intravascular coagulation?" Trends in Pharmacological Sciences 26, no. 6 (June 2005): 327–32. http://dx.doi.org/10.1016/j.tips.2005.04.002.
Full textJain, Rohit, Shweta Tikoo, and Wolfgang Weninger. "Recent advances in microscopic techniques for visualizing leukocytes in vivo." F1000Research 5 (May 19, 2016): 915. http://dx.doi.org/10.12688/f1000research.8127.1.
Full textCostanzo, Vincenzo, and Michele Costanzo. "Intravital Imaging with Two-Photon Microscopy: A Look into the Kidney." Photonics 9, no. 5 (April 27, 2022): 294. http://dx.doi.org/10.3390/photonics9050294.
Full textVinegoni, Claudio, Aaron D. Aguirre, Sungon Lee, and Ralph Weissleder. "Imaging the beating heart in the mouse using intravital microscopy techniques." Nature Protocols 10, no. 11 (October 22, 2015): 1802–19. http://dx.doi.org/10.1038/nprot.2015.119.
Full textHickey, Michael J., and Paul Kubes. "Use of Intra Vital Microscopy to Analyze Leukocyte Rolling and Adhesion In Vivo." Microscopy and Microanalysis 3, S2 (August 1997): 323–24. http://dx.doi.org/10.1017/s1431927600008503.
Full textTorres Filho, Ivo P., James Terner, Roland N. Pittman, Leonardo G. Somera, and Kevin R. Ward. "Hemoglobin oxygen saturation measurements using resonance Raman intravital microscopy." American Journal of Physiology-Heart and Circulatory Physiology 289, no. 1 (July 2005): H488—H495. http://dx.doi.org/10.1152/ajpheart.01171.2004.
Full textLindbom, Lennart, and Ellinor Kenne. "Imaging inflammatory plasma leakage in vivo." Thrombosis and Haemostasis 105, no. 05 (2011): 783–89. http://dx.doi.org/10.1160/th10-10-0635.
Full textHato, Takashi, Allon N. Friedman, Henry Mang, Zoya Plotkin, Shataakshi Dube, Gary D. Hutchins, Paul R. Territo, et al. "Novel application of complementary imaging techniques to examine in vivo glucose metabolism in the kidney." American Journal of Physiology-Renal Physiology 310, no. 8 (April 15, 2016): F717—F725. http://dx.doi.org/10.1152/ajprenal.00535.2015.
Full textANTONIOS, Tarek F. T., Fraser E. M. RATTRAY, Donald R. J. SINGER, Nirmala D. MARKANDU, Peter S. MORTIMER, and Graham A. MACGREGOR. "Maximization of skin capillaries during intravital video-microscopy in essential hypertension: comparison between venous congestion, reactive hyperaemia and core heat load tests." Clinical Science 97, no. 4 (September 16, 1999): 523–28. http://dx.doi.org/10.1042/cs0970523.
Full textDissertations / Theses on the topic "Intravital microscopy techniques"
Alaoui, Lasmaili Karima El. "Caractérisation au moyen d'outils mathématiques des effets vasculaires du bevacizumab à des fins d'optimisation des protocoles thérapeutiques dans le cas des tumeurs cérébrales." Thesis, Université de Lorraine, 2017. http://www.theses.fr/2017LORR0023/document.
Full textThe main aim of this work was to characterize the effects of the anti-VEGF Bevacizumab (Avastin) on the tumor vascular network, in vivo, over time, thanks to the skin fold chamber model on the nude mouse. Images of the vascular network obtained using intravital microscopy were analyzed par a dedicated image processing algorithm developed within our research team, allowing to highlight the morphological modifications induced by the treatment and to isolate discriminating parameters of the vascular "normalization", by comparison to healthy vascular networks. Le vascular "normalization" period detected with our tool was comforted by the analysis of the functionality of the blood vessels over time, in vivo and by an immunohistochemical analysis of the blood vessels and of the tumor tissue. In preliminary in vivo experiments, we tried to verify the hypothesis of the benefits of an anti-VEGF treatment prior to photodynamic therapy (PDT) on glioblastoma xenografts implanted subcutaneously or in the skin fold chamber. The efficacy of PDT is described as being dependent on tumor oxygenation and on the distribution of the photosensitizing agent within the tumor. In paralel to this work, we tried as a pluridisciplinary team to develop a mathematical model of the tumor response to bevacizumab using biological data obtained on the same in vivo model et that will allow in the future to simulate the response for different doses and different treatment durations, for the optimization of therapeutic protocols
DUSI, SILVIA. "Role of integrins in the trafficking of Th1 and Th17 cells in the central nervous system during experimental autoimmune encephalomyelitis." Doctoral thesis, 2017. http://hdl.handle.net/11562/961282.
Full textMultiple sclerosis (MS) is a chronic disabling autoimmune inflammatory demyelinating disease of the central nervous system (CNS). The migration of autoreactive T cells from the blood into the CNS and their reactivation through antigen presentation by local antigen presenting cells (APCs) represent critical events in the pathogenesis of MS and its animal model, the experimental autoimmune encephalomyelitis (EAE). The responses to potential antigens inside the CNS require long-range migration of cells, short-range communication and direct cell-cell contact with APCs. The main goal of this study was to investigate the role of L2 (LFA-1) and 4 (VLA-4 and 47) integrins in the migration and motility behavior of Th1 and Th17 cells, which represent key players in the induction of EAE, using intravital microscopy approaches. Intravital microscopy techniques allow the visualization of T cell migration and reactivation in the spinal cord (SC), which represents the main inflammation site during EAE. By using epifluorescence intravital microscopy (IVM) we first studied the roles of 4 and LFA-1 integrins in Th1 and Th17 cell adhesion in the pial vessels of spinal cord (SC) venules in mice immunized with MOG35-55 peptide during the preclinical phase, disease onset and chronic phase of disease. We used an EAE model by immunization of C57BL/6 mice with MOG35-55 peptide. MOG35-55-specific Th1 and Th17 cells were produced in vitro from 2D2 TCR transgenic mice, labeled with fluorescent dyes and intravenously injected in immunized mice before imaging. Our results underlined a selective role for LFA-1 integrin in Th1 cell recruitment in inflamed SC vessels during the early phases of the disease but not during the chronic phase. Moreover, blocking antibodies against the 4subunit, but not blockade of 47 integrin greatly inhibited rolling and firm adhesion of Th1 cells in the SC venules during all disease phases, suggesting that VLA-4 is the major molecule involved in Th1 cell adhesion in the SC venules during EAE. Interestingly, blockade of 47 integrin led to a significant reduction of firm adhesion in Th17 cells at the onset and chronic phase of EAE indicating a selective role of 47 integrin in the recruitment of Th17 cells in the inflamed CNS. Taking advantage of two-photon laser microscopy (TPLM) approach we next investigated the motility behavior of fluorescently labeled Th1 and Th17 cells within SC parenchyma during different disease phases. Our results showed a massive infiltration of Th1 and Th17 cells in the CNS parenchyma at disease peak, whereas the migration of these cells during other phases of disease was significantly lower. Furthermore, Th1 and Th17 cells displayed significant differences in the directional component, with Th1 cells moving faster in straight directions covering long distances deep in the SC parenchyma, whereas Th17 cells moved around in a specific volume of tissue in a stop and go mode. Notably, the blockade of LFA-1 integrin drastically affected the dynamics of Th1 cells leading to a reduction in velocity and interfering with their straight-line motility pattern. Moreover, Th17 cells displayed a drastic reduction of velocity in the presence of a blocking anti-LFA-1 antibody. The analysis of cellular morphology suggested that LFA-1 is actively involved in the cytoskeleton rearrangements necessary for T cell amoeboid migration inside the CNS, but had no role in the cytoskeleton dynamics in Th17 cells. Notably, 4integrins had no role in Th1 cells motility, but drastically reduced the dynamics of Th17 cells inside the SC parenchyma. To check the therapeutic relevance of our intravital microscopy findings, we performed intrathecal injection of anti-LFA-1 or anti-47 antibodies at disease onset and observed a significant inhibition of EAE progression in mice immunized with MOG35-55 peptide. Collectively, our data demonstrate that LFA-1 integrin differently controls intraparenchymal Th1 and Th17 cells dynamics, whereas 47 integrin is selectively involved in Th17 cell trafficking in the CNS during EAE. Furthermore, our results suggest that interfering with the molecular mechanisms controlling intraparenchymal dynamics of activated T cells may represent a new therapeutic strategy for CNS autoimmune diseases.
Corridon, Peter R. "Hydrodynamic delivery for the study, treatment and prevention of acute kidney injury." Thesis, 2014. http://hdl.handle.net/1805/4603.
Full textAdvancements in human genomics have simultaneously enhanced our basic understanding of the human body and ability to combat debilitating diseases. Historically, research has shown that there have been many hindrances to realizing this medicinal revolution. One hindrance, with particular regard to the kidney, has been our inability to effectively and routinely delivery genes to various loci, without inducing significant injury. However, we have recently developed a method using hydrodynamic fluid delivery that has shown substantial promise in addressing aforesaid issues. We optimized our approach and designed a method that utilizes retrograde renal vein injections to facilitate widespread and persistent plasmid and adenoviral based transgene expression in rat kidneys. Exogenous gene expression extended throughout the cortex and medulla, lasting over 1 month within comparable expression profiles, in various renal cell types without considerably impacting normal organ function. As a proof of its utility we by attempted to prevent ischemic acute kidney injury (AKI), which is a leading cause of morbidity and mortality across among global populations, by altering the mitochondrial proteome. Specifically, our hydrodynamic delivery process facilitated an upregulated expression of mitochondrial enzymes that have been suggested to provide mediation from renal ischemic injury. Remarkably, this protein upregulation significantly enhanced mitochondrial membrane potential activity, comparable to that observed from ischemic preconditioning, and provided protection against moderate ischemia-reperfusion injury, based on serum creatinine and histology analyses. Strikingly, we also determined that hydrodynamic delivery of isotonic fluid alone, given as long as 24 hours after AKI is induced, is similarly capable of blunting the extent of injury. Altogether, these results indicate the development of novel and exciting platform for the future study and management of renal injury.
Book chapters on the topic "Intravital microscopy techniques"
Turk, Madison, Jeff Biernaskie, Douglas J. Mahoney, and Craig N. Jenne. "Intravital Microscopy Techniques to Image Wound Healing in Mouse Skin." In Methods in Molecular Biology, 165–80. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2051-9_10.
Full textStein, Jens V. "Intravital Microscopy and In Vitro Flow Chamber: Techniques to Study Leukocyte Adhesion Under Flow and in Real Time." In Leukocyte Trafficking, 455–71. Weinheim, FRG: Wiley-VCH Verlag GmbH & Co. KGaA, 2006. http://dx.doi.org/10.1002/352760779x.ch21.
Full textR. Corridon, Peter. "Fluorescent Dextran Applications in Renal Intravital Microscopy." In Fluorescence Imaging - Recent Advances and Applications [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.107385.
Full textA. Matthay, Zachary, and Lucy Zumwinkle Kornblith. "Platelet Imaging." In Platelets. IntechOpen, 2020. http://dx.doi.org/10.5772/intechopen.91736.
Full textConference papers on the topic "Intravital microscopy techniques"
Piyawattanametha, W., H. Ra, M. J. Mandella, J. T. C. Liu, E. Gonzalez, R. Kaspar, G. S. Kino, O. Solgaard, and C. H. Contag. "In vivo Clinical and Intravital Imaging with MEMS based Dual-Axes Confocal Microscopes." In Novel Techniques in Microscopy. Washington, D.C.: OSA, 2009. http://dx.doi.org/10.1364/ntm.2009.nwc1.
Full textCheung, Anthony T. W. "Intravital microscopy: A laser and computer assisted approach." In ICALEO® ‘85: Proceedings of the Medicine and Biology; Optical Techniques for Measurement and Control; and Spectroscopy, Photochemistry and Scientific Measurement Conferences. Laser Institute of America, 1985. http://dx.doi.org/10.2351/1.5057690.
Full text