Academic literature on the topic 'Intra-osseous defect'

Seven intra-articular fractures in five patients with partial bone loss at the PIP joint were reconstructed using a graft of costal cartilage. In all cases there were total or partial cartilagenous defects of the proximal phalangeal side of the joint. Early treatment of two joints, using only costal cartilage grafts, resulted in bony ankylosis due to necrosis of the grafted cartilage. In five joints the grafted cartilage included osseous portions using the costo-osteochondral junction, leading to an average range of movement of 64° with satisfactory clinical results. The technique is a useful alternative to other forms of arthroplasty or arthrodesis, and can provide satisfactory functional results when there is a partial defect of the head of the proximal phalanx.

AIM: This study aimed to evaluate and to compare the regenerative power of simvastatin, Demineralized Freeze-Dried Bone Allograft (DFDBA) allograft, platelets rich Fibrin (PRF), and a combination of these materials radiographically and histologically in the intra-bony periodontal defects in white New Zealand rabbits. MATERIALS AND METHODS: This study was conducted on 54 defects in 27 adult male rabbits (n = 27) which were divided into three groups according to the follow-up preplanned scheduled for 1, 2, and 3 weeks. The selected materials were induced as following: A=DFDBA, B=Simvastatin, C= PRF, D=A+C, E=B+C, and F=negative (control group). The intra-bony periodontal defects were induced as the form of one osseous wall defect of 10 mm height, 4 mm depth between the first and the second molars. Then, samples were prepared for histological evaluation. Radiographic assessment was done using computed tomography radiography which was carried at different time intervals as the following baseline, 1, 2, and 3 weeks later. Statistical analysis was performed using ANOVA. RESULTS: After evaluating the results, macroanatomy, radiographically, and histologically, it is thus confirmed that DFDBA allograft combined with PRF create the best bone regenerative results, followed by DFDBA, Simvastatin, simvastatin+ PRF, control group, and finally PRF. CONCLUSION: All of the materials examined in this study showed different percentage in terms of bone density and bone regenerative effects. However, the best results for bone density of the DFDBA + PRF group were recorded after 3 weeks. Thus, the study concludes that a combination of DFDBA + PRF reflects the best properties of both materials in terms of bone density results of the defect. Such results are particularly significant for the selection of bone regeneration materials, and generally, for periodontal regeneration.

ntroduction: Periodontitis is a multifactorial disease, which, when not adequately treated, is followed with progressive attachment loss which leads to tooth mobility and eventually tooth loss. Periodontal regenerative surgery aims to regenerate and reconstruct the lost periodontal tissue. Regeneration with novabone putty has shown to be effective in reducing probing pocket depth, gain in clinical attachment level and increase in horizontal bone level. Aim: This interventional clinical trial was to evaluate the osseous regenerative potential of a calcium phosphosilicate bioactive glass NOVABONE TM in the treatment of horizontal bone defects. Materials and method: A total of 20 sites with horizontal bone defect was treated with open flap debridement with intra marrow penetration and novabone putty. Statistical analysis: Plaque index, gingival index and radiographic determination were analysed by paired t test. Probing pocket depth and clinical attachment levels were analysed by Wilcoxon Signed Ranks Test. Result: There was significant reduction seen in plaque index, gingival index, probing pocket depth and radiographic crestal level when compared between baseline and 12 months respectively (2.48 ± 0.44, 2.92 ± 0.39, 6.80 ± 0.89, 8.29 ± 0.87) and (1.89 ± 0.42, 1.92± 0.51, 3.00± 0.67, 5.48 ± 0.89) and significant gain in clinical attachment level from 4.40 ± 0.96 to 1.20 ± 0.91. Conclusion: The present study showed novabone putty significantly improved the clinical parameters in horizontal bone defects.

SummaryAutologous bone marrow plays an increasing role in the treatment of bone, cartilage and tendon healing disorders. Cell-based therapies display promising results in the support of local regeneration, especially therapies using intra-operative one-step treatments with autologous progenitor cells. In the present study, bone marrow-derived cells were concentrated in a point-of-care device and investigated for their mesenchymal stem cell (MSC) characteristics and their osteogenic potential.Bone marrow was harvested from the iliac crest of 16 minipigs. The mononucleated cells (MNC) were concentrated by gradient density centrifugation, cultivated, characterized by flow cytometry and stimulated into osteoblasts, adipocytes, and chondrocytes. Cell differentiation was investigated by histological and immunohistological staining of relevant lineage markers. The proliferation capacity was determined via colony forming units of fibroblast and of osteogenic alkaline-phosphatase-positive-cells.The MNC could be enriched 3.5-fold in nucleated cell concentrate in comparison to bone marrow. Flow cytometry analysis revealed a positive signal for the MSC markers. Cells could be differentiated into the three lines confirming the MSC character. The cellular osteogenic potential correlated significantly with the percentage of newly formed bone in vivo in a porcine metaphyseal long-bone defect model.This study demonstrates that bone marrow concentrate from minipigs display cells with MSC character and their osteogenic differentiation potential can be used for osseous defect repair in autologous transplantations.

Treatment of Calcaneus fractures needs improvement of modern methods. The purpose of the work is to reveal the structural and functional advantages of the results of surgical treatment of patients with intra-articular fractures of the calcaneus with the use of bone plastic. Material and methods. Case histories of 51 patients treated surgically at the clinical bases of the Department of General Surgery between 2014–2019, retrospectively studied for impressionable intraatricular fractures of 53 calcaneus bones with displacement. The average age of trauma victims was 34.7 ± 1.6 years (26 to 51 years), women - 9 (17.64%), men - 42 (82.35%). All patients operated by open reduction and osteosynthesis (group A; n = 10; 19.6%), and in combination with an osteoplasty by autograft from the iliac crest, biocermic implant (group B; n = 37; 80.4%). Results. At 18 months, excellent results (100–90 points for AOFAS) were achieved in 14 (41.6% - group A; 58.4% - group B) patients, good (89–80 points for AOFAS) in 23 (42, 8% - group A; 57.2% - group B) patients, satisfactory (79-70 points for AOFAS) - in 10 (60.0% - group A; 40.0% - group B) patients, unsatisfactory (less from 70 AOFAS points) - in 4 (75.0% - group A; 25.0% - group B) patients. Conclusions. The use of a bio-ceramic bone implant has proven to be a promising direction for osteoplastic interventions with good deformation resistance in the long term. Replacement of bone defect with an auto-osseous graft at the reference osteoplasty in separate terms showed higher bone density compared to none, but less than when using a bioceramic filler Post-operative deformity of the calcaneus testifies to the difficulties of treatment of this pathology, which requires further search for the most optimal materials for replacement of the bone cavity in order to achieve the possibility of early mobilization, preservation of the anatomic structure of the calcaneus, congruence of joint surfaces, prevention of complications.

Hill-Sachs lesions were first described in 1940 as grooved defect in the posterior aspect of the humeral head associated with traumatic anterior glenohumeral dislocation. The reported incidence of Hill-Sachs lesions following traumatic anterior instability events ranges from 60% to 90%. Despite recognition of the Hill-Sachs lesion for more than 75 years, most shoulder stabilization procedures have focused on repairing or augmenting the torn or deficient anterior soft tissues and glenoid bone in hopes of preventing engagement of the posterior humeral head defect. To help identify lesions that are important causes of instability, both Palmer and Widen and Burkhart and De Beer described the ‘‘engaging’’ Hill-Sachs lesion, which refers to one that engages the rim of the glenoid when the shoulder is physiologically abducted and externally rotated. Engaging Hill-Sachs lesions lead to recurrent instability, and a high rate of failure when treated with arthroscopic Bankart repair alone. There are two methods to assess the Hill-Sachs lesion related with surgical decision making for concomitant remplissage procedure. One method is dynamic examination. During arthroscopic surgery, the relative relationship between the Hill-Sachs lesion and the glenoid can be assessed. The important thing is that this dynamic examination should be performed after the Bankart repair. The disadvantage of this method is that there is a risk of damaging the repair during the dynamic examination. The second method is to use the ‘glenoid track’ concept. The width of the glenoid track, defined as the distance between the medial margin of the glenoid track and the medial margin of the footprint of the rotator cuff was 83% of the glenoid width when the arm was at 90° of abduction in live shoulders. Our institution use en face views of both glenoids and the posterior view of the involved humeral head on 3D CT. First, we measure the width of the intact glenoid and calculate 83% of the glenoid width(0.83D). Then, this 83% value (0.83D) is applied to the involved glenoid en face view. If there is a bony defect of the glenoid, the defect width ‘d’ needs to be subtracted from the 83% value (0.83D) to obtain the true width of the glenoid track (0.83D - d). We apply this width (0.83D - d) to the posterior view of the humeral head. If the medial margin of the Hill-Sachs le stays within the glenoid track, there is no risk that this Hill-Sachs lesion engages with the anterior rim of the glenoid. If the Hill-Sachs lesion extends more medially over the medial margin of the glenoid track, there is a risk of engagement and dislocation. The former used to be called ‘on-track HSL’ and the latter ‘off-track Hill-Sachs lesion’. Based on the on-track/off-track concept, treatment strategy is as follows. For shoulders with on-track Hill-Sachs lesion and glenoid bone loss of < 25%, Bankart repair alone is sufficient. With on-track Hill-Sachs lesion and glenoid bone loss of ≥ 25%, the glenoid bone loss needs to be fixed, for example by the Latarjet procedure. With off-track Hill-Sachs lesion and the glenoid bone loss of < 25%, Bankart repair plus remplissage is needed. In addressing recurrent anterior shoulder instability, surgical decision making for additional remplissage procedure is inevitable. There are two methods: one is glenoid track method which can be employed in preoperative evaluation. The other is assessment of engaged Hill-Sachs lesion during arthroscopic evaluation. Which one do you prefer? References Hill, H.A. and M.D. Sachs, The grooved defect of the humeral head: a frequently unrecognized complication of dislocations of the shoulder joint. Radiology, 1940. 35(6): p. 690-700. Burkhart, S.S. and J.F. De Beer, Traumatic glenohumeral bone defects and their relationship to failure of arthroscopic Bankart repairs: significance of the inverted-pear glenoid and the humeral engaging Hill-Sachs lesion. Arthroscopy: The Journal of Arthroscopic & Related Surgery, 2000. 16(7): p. 677-694. Flatow, E.L. and J.J. Warner, Instability of the shoulder: Complex problems and failed repairs: Part 1. Relevant biomechanics, multidirectional instability, and severe loss of glenoid and humeral bone. Journal of Bone and Joint Surgery, 1998. 80(1): p. 122. Lynch, J.R., et al., Treatment of osseous defects associated with anterior shoulder instability. Journal of shoulder and elbow surgery, 2009. 18(2): p. 317-328. Buza, J.A., 3 rd, et al., Arthroscopic Hill-Sachs remplissage: a systematic review. J Bone Joint Surg Am, 2014. 96(7): p. 549-55. Spatschil, A., et al., Posttraumatic anterior-inferior instability of the shoulder: arthroscopic findings and clinical correlations. Archives of orthopaedic and trauma surgery, 2006. 126(4): p. 217-222. Yiannakopoulos, C.K., E. Mataragas, and E. Antonogiannakis, A comparison of the spectrum of intra-articular lesions in acute and chronic anterior shoulder instability. Arthroscopy: The Journal of Arthroscopic & Related Surgery, 2007. 23(9): p. 985-990. Itoi, E., ‘On-track’ and ‘off-track’ shoulder lesions. EFORT Open Rev, 2017. 2(8): p. 343-351. Parke, C., et al. Arthroscopic remplissage for humeral defect in anterior shoulder instability: is it needed. in 39th annual meeting of Japan Shoulder Society, Tokyo. 2012. Yamamoto, N., et al., Contact between the glenoid and the humeral head in abduction, external rotation, and horizontal extension: a new concept of glenoid track. Journal of shoulder and elbow surgery, 2007. 16(5): p. 649-656. Omori, Y., et al., Measurement of the glenoid track in vivo as investigated by 3-dimensional motion analysis using open MRI. The American journal of sports medicine, 2014. 42(6): p. 1290-1295. Di Giacomo, G., E. Itoi, and S.S. Burkhart, Evolving concept of bipolar bone loss and the Hill-Sachs lesion: from “engaging/non-engaging” lesion to ”on-track/off-track” lesion. Arthroscopy: The Journal of Arthroscopic & Related Surgery, 2014. 30(1): p. 90-98. Locher, J., et al., Hill-Sachs off-track lesions as risk factor for recurrence of instability after arthroscopic Bankart repair. Arthroscopy: The Journal of Arthroscopic & Related Surgery, 2016. 32(10): p. 1993-1999. Shaha, J.S., et al., Clinical validation of the glenoid track concept in anterior glenohumeral instability. JBJS, 2016. 98(22): p. 1918-1923. Yamamoto, N., et al., The stabilizing mechanism of the Latarjet procedure: a cadaveric study. JBJS, 2013. 95(15): p. 1390-1397. Connolly, J., Humeral head defects associated with shoulder dislocation: their diagnostic and surgical significance. Instr. Course Lect., 1972. 2: p. 210-218. Purchase, R.J., et al., Hill-Sachs “remplissage”: an arthroscopic solution for the engaging Hill-Sachs lesion. Arthroscopy: The Journal of Arthroscopic & Related Surgery, 2008. 24(6): p. 723-726. Boileau, P., et al., Arthroscopic Hill-Sachs Remplissage with Bankart Repair: Strategy and Technique. JBJS Essent Surg Tech, 2014. 4(1): p. e4.

Dear Editor, Reconstruction of mandibular defects resulting from ablative surgery for benign and malignant tumors remains a reconstructive challenge. For the past decade, the fibular free flap has been the workhorse for large mandibular defects because of its length, versatility, and ability to be harvested with a skin paddle for soft tissue closure. Although its success rate has continuously improved to almost 95%, donor site morbidity remains a matter of concern.1,2 Bone grafts are already widely used in dental surgery but only as fillers for chipped or marginal defects and not for large segmental mandibular defects. We present a new technique of reconstructing segmental mandibular defects using bone grafts combined with autologous platelet-rich fibrin (PRF), a biomaterial derived intra-operatively from the patient that incorporates leukocytes, platelets, growth factors, and a wide range of glycoproteins in a dense fibrin matrix. Moreover, we describe the essential role of PRF in bone healing and regeneration that offers an invaluable reconstructive option that is free of donor site morbidity without sacrificing the main goal of reconstruction in restoring both form and function. Keywords: Mandibular reconstruction; segmental mandibular defect; bone graft; autologous Platelet-Rich Fibrin (PRF) MATERIALS AND METHODS Subject and Indications A 23-year-old male underwent reconstruction with allogenic bone graft in combination with autologous platelet-rich fibrin (PRF) for a large segmental angle to parasymphyseal mandibular defect. (Figure 1) As in this example, the authors’ technique for segmental mandibular defect reconstruction using bone grafts with PRF was best performed as a second stage procedure following tumor ablation to prevent contamination from oral cavity secretions. As with any elective procedure, a thorough review of the medical history, control of systemic disease and informed consent were necessary. Patients with contraindications for fibular free flaps, such as history of peripheral vascular disease, unfavorable imaging of the lower extremity, venous insufficiency, and anomalous lower extremity vasculature may benefit from this technique.3 Patients who had failed mandibular reconstruction with other methods such as those reconstructed with single alloplastic material, titanium plate, non-vascularized autologous bone graft, or free flaps are likewise candidates for this option. Procedure A submandibular incision was made and a sub-platysmal flap was raised to expose the entire length of the mandibular defect. Apart from the preservation of vital structures in the area, it was important not to violate the oral mucosa to prevent contamination of the reconstruction site. A cortico-cancellous allogenic bone graft (Maxgraft®, Botiss Medical AG , Berlin, Germany) was fitted to the mandibular defect and anchored with bicortical screws (2.5; 2.8 mm, lengths 14-20 mm) to a pre-bended 2.5 mm reconstruction titanium plate (Modus Reco 2.5, Medartis, Hochbergerstrasse Basel Switzerland). The remaining gaps between the inlaid grafts were filled with the remaining cancellous bone and biomimetic composite materials. (Figure 2) Although allogenic bone grafts with cortical and cancellous components are recommended for mandibular ramus and condylar reconstruction as these regions are composed of nearly 100% cortical bone, xenogenic (Cerabone®, Botiss Medical AG , Berlin, Germany) or combined alloplastic material- (Maxresorb®, Botiss Medical AG , Berlin, Germany) bone grafts may be used in other regions. (Figure 3) Meanwhile, venous blood was simultaneously drawn from the patient and placed in a 10 cc glass collecting tube for single centrifuge processing using a PC-O2 centrifuge (PC-O2, Process, Nice, France). The specific centrifuge processes 8 uncoated tubes using a standard protocol specially manufactured for processing PRF using 33° tube angulation at 2700 RPM’s, soft spin for 12 minutes.4 (Figure 4) At the end of the centrifugation process, three distinct fractions of blood components were produced where the intermediate fraction composed of dense PRF clot was used. The other blood components separated by the centrifugation process- serum or platelet-poor plasma (PPP) and red blood cell concentrates, were respectively situated in the superficial and bottom layers of the collecting tube. The PRF clots were then transferred to a PRF processing box (PRF Box®, Process, Nice, France) to prepare standardized membranes and harvest the PRF exudates in a sterile environment.5 (Figure 5) Collagen material of native pericardium (GTR/GBR) membrane (Jason® membrane, Botiss Medical AG, Berlin, Germany) was placed underneath the graft recipient site and the processed PRF membranes were layered over the graft recipient site to stimulate osteoblastic differentiation and neoangiogenesis.6 The entire recipient site was then enveloped with the collagen material, mechanically securing the autologous PRF in contact with the bone grafts. (Figure 6) This established a membrane barrier for guided bone regeneration (GBR) and guided tissue regeneration (GTR) by preventing growth of undesired cells inside the neomandible and allowing osteogenesis and angiogenesis.7 Commercially available collagen biomaterials vary from native collagen membrane to enhanced Ca/P collagen composite materials such as the 3D-stable collagen graft (Mucoderm®, Botiss Medical AG, Berlin, Germany) with larger available sizes for long mandibular defect coverage. The skin was closed in the usual manner and the patient was initially maintained on a liquid diet, progressing to a soft diet over 2-4 weeks. Plain panoramic radiographs after a week confirmed proper alignment of the bone grafts and monthly radiographic series was recommended for the first 6 months after reconstruction. RESULTS Monthly panoramic radiographs for the first 6 months after reconstruction showed absence of bone resorption. A 3D reconstruction CT imaging of the mandible was done after 10 months for placement of three osteo-integrated dental implants. (Figure 7) Bone biopsies were also taken in conjunction with placement of dental implants, and sent to the University of Bonn, Germany for histologic evaluation. (Figure 8) The trichrome-stained specimens showed new mineralized tissues consisting of woven bone characterized by high numbers of distributed osteocytes and irregularly arranged fiber bundles within the new bone matrix confirming bone regeneration. (Figure 9) The latest panoramic radiograph of the patient at 26 months after surgery showed absence of gaps between the bone grafts and their junction with the normal mandible, evincing complete bone regeneration and a successful mandibular reconstruction. (Figure 10) A total of 14 cases of segmental mandibular defects have been reconstructed by the authors using the particular technique from January 2011 to February 2013 with 100% success rate and will be reported as a series in the near future. DISCUSSION Advancements in mandibular reconstruction have continued to develop over the past decades. The use of alloplastic materials like titanium plating implants for repairing mandibular defects provided patients with rapid rigid mandibular restoration but was limited by numerous complications such as infection, plate extrusion, and subsequent failure. Recently, the concept of distraction osteogenesis, involving bone distraction with an external mechanical device and progressive lengthening of the bone to allow a gap of new bone during the consolidation phase has also been used for mandibular reconstruction but has been limited by poor scar formation, delayed return to function, and inadequate formation of desired bone length.8 The advent of microvascular reconstructive surgery enabled the transfer of vascularized osseous flaps with the most commonly used fibular free flap showing superior results over non-vascularized bone transfer and better quality of life outcome. However, it did not remain free of donor-site morbidities.9 Tissue engineering led to the development and use of bone grafts that hold promise for the future of head and neck repair.10 Numerous clinical studies demonstrated the utility of tissue engineering in developing bone grafts for mandibular defect reconstruction.11 Such have already been widely used over the past decades in oro-maxillary and dental reconstruction, including the recombinant bone morphogenetic protein (rhBMP-2) that is now used with great success in cleft palate repair, alveolar ridge augmentation, and sinus lift procedures.12 Autogenous bone grafts derived from the patient work through osteogenesis, osteoinduction and osteoconduction. However, such are not recommended because apart from enabling a donor site morbidity-free technique, they are best harvested as microvascular flaps. Allogenic bone grafts on the other hand are cadaveric processed grafts that may be cortical, trabecular, or combined in composition and have both osteoconductive and osteinductive properties. Xenografts or processed animal bone graft are a subgroup of the synthetically manufactured alloplasts known to form new bones from their osteoconductive activities. The use of autologous PRF is already widely used in combination with bone grafts for dental surgeries but not for large mandibular defects.13 Our reconstructive technique using bone grafts for large segmental mandibular defects emphasizes the important role of PRF with its intrinsic factors and leukocyte contents that release high amounts of growth factors such as TGBß1, PDGF-AB, VEGF and matrix glycoproteins.14 Collagen membrane used to envelop the entire recipient site creates a membrane barrier to prevent the growth of soft tissues and allow angiogenesis within the neomandible.15 Fascia lata may be used as a membrane barrier but defeats the authors’ goal of an absolute donor site morbidity-free procedure. Overall, this particular technique along with gentle tissue handling and avoidance of oral cavity contamination for reconstructing large mandibular defects has been found to enhance bone regeneration capable for osteo-integrated dental implantation. Generally, the harvesting and processing of autologous PRF is simple and inexpensive. Its use with bone grafts is a good substitute for segmental mandibular reconstruction in patients with contraindications to free flap procedures or in cases where patients simply wish to be free from any donor-site morbidity. However, this technique is limited to defects secondary to trauma and ablation of benign conditions as bone regeneration is expected in approximately 6 to 9 months. Mandibular defects following resection of malignant oral neoplasms are still best reconstructed with fibular free flaps as radiation therapy is warranted at the soonest possible time. Histologic validation of bone regeneration and osteoblastic activity index for the 13 other cases performed by the authors using this particular technique necessitates bone research centers that are capable of advanced bone analysis, and none are locally-available at this time. Meanwhile, this technique of combining autologous PRF in bone grafting remains an innovative and invaluable option for mandibular reconstruction today.

Introduction. The primary goal of bone regeneration procedures with application of various regenerative biologic agents and biomaterials is to facilitate the formation of periodontal tissues lost as a result of periodontitis. Objective. The aim of the study was to compare clinical outcome of the guided tissue regeneration (GTR) treatment with the use of ?-tricalcium phosphate and with bovine bone matrix in human deep intra-osseous defects. Methods. Twenty-one systemically healthy subjects with moderate to advanced periodontitis, between 30 and 56 years of age, 11 females and 10 males, were selected. Patients having two similar inter-proximal defects with pocket probing depths following initial therapy greater than 5 mm were recruited for the study. Experimental sites were grafted with pure ?-tricalcium phosphate biomaterial (Cerasorb?) and a biomembrane, while control sites were treated with bovine-bone hydroxiapatite xenograft (Bio-oss?) and a biomembrane. Immediately before surgery and 12 months after surgery, pocket probing depth (PPD), epithelial attachment level (EAL) and gingival recession (GR) were evaluated. Results. In the experimental group PPD amounted to 6.76?0.83 mm before surgery, and decreased significantly to 2.67?0.48 mm 12 months following surgery, while in the control group PPD significantly decreased from 7.14?0.65 mm presurgically to 2.85?0.57 mm postsurgically. After one year, EAL gain was 2.76?0.99 mm in the experimental group, and 3.24?0.16 mm in the control group. After twelve months postoperatively GR amounted to 1.33?0.79 mm in the experimental group and to 1.05?0.80 mm in the control group. No statistically significant differences for PPD reduction, EAL gain and GR increase were detected between the groups. Conclusion. Results from the present study indicate that GTR treatment of deep intra-osseous defects with Bio-oss? and Cerasorb? resulted in clinically and statistically significant improvement of EAL gain and PPD reduction. A GR was slightly increased, with no statistical significance.

Background: Plasma Rich in Growth Factors (PRGF) is a mixture of autologous proteins and growth factors, prepared from a certain volume of platelet-rich plasma obtained from a small volume of blood, which does not contain leukocytes. Aim: The aim of the study was to evaluate the clinical and radiological efficacy of Plasma rich in growth factors (PRGF) in adjunct to open flap debridement (OFD) and intra-marrow penetration (IMP) compared to OFD and IMP alone for management of periodontal intra-osseous defects in periodontitis patients. Material and methods: Twenty patients with forty contra-lateral sites presenting with >5mm pocket depth and >3mm of intra-bony defect component were recruited in this double blind, split-mouth randomized controlled trial. The experimental site was surgically treated with PRGF in adjunct to OFD and IMP; and the control sites were treated with OFD and IMP alone. The clinical parameters like site specific plaque index (SSPI), site specific gingival index (SSGI), probing pocket depth (PPD), relative attachment level (RAL), gingival marginal level (GML), site-specific bleeding on probing (SSBOP) were recorded at baseline, 3, 6 and 9 months. The radiological parameters like intra-bony defect depth (IBDD), intra-bony defect area (IBDA) and percentage intra-bony defect area fill (%IBDAF) was recorded at baseline, 6 and 9 months. The patient reported outcomes on swelling, bleeding and level of pain in the area treated were also assessed at day 1 to day7. Results: No significant difference was observed for PI, GI and PPD. A Significant favorable improvement in GML was observed in PRGF treated group at all time points, suggesting continuous vertical creeping of the free gingival margin in the PRGF group at 3, 6 and 9 months. The clinical attachment gain (CAG) was significantly higher in the PRGF group at 3 months (p=0.005) and borderline significance at 6 months (p=0.067). The linear radiographic bone gain, i.e, change in IBDD, was significantly higher in the PRGF treated group at 6 months (p=0.02). At 6 months, the PRGF was significantly superior to the OFD (50% Vs 15%) in the number of sites that achieved CAG by 1.5 mm and linear radiographic bone gain of 1.0 mm. At 9 months, the PRGF was border-line significance than the OFD (83% Vs 50%) in the number of sites that achieved CAG by 1.5 mm and linear radiographic bone gain of 1.0 mm. Conclusion: PRGF was found to beneficial in terms of improved clinical attachment gain and radiographic linear bone gain, when used in adjunct to OFD and IMP for management of periodontal intra-osseous defects.