Relevant bibliographies by topics / Interference reflection microscopy (IRM)

Academic literature on the topic 'Interference reflection microscopy (IRM)'

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Published: 7 July 2024
Last updated: 7 July 2024

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Journal articles on the topic "Interference reflection microscopy (IRM)":

1

Verschueren, H. "Interference reflection microscopy in cell biology: methodology and applications." Journal of Cell Science 75, no. 1 (April 1, 1985): 279–301. http://dx.doi.org/10.1242/jcs.75.1.279.

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Since its introduction into cell biology by Curtis in 1964, interference reflection microscopy (IRM) has been used by an increasing number of researchers to study cell-substrate interactions in living cells in culture. With the use of antiflex objectives, high-contrast IRM images can now be readily obtained. From the different theories on image formation in IRM that have been put forward, it can be seen that a zero-order interference pattern is generated at high illuminating numerical aperture. This yields information on the closeness of contact between cell and substrate, with only minor perturbation by reflections from the dorsal cell surface. Therefore, the proper use of illuminating apertures is crucial. Nevertheless, IRM images have to be interpreted with caution, especially under thin cytoplasmic sheets. Quantitative IRM is possible only with a mathematical model for finite illuminating aperture interferometry and with an independent measurement of cell thickness for values up to 1 micron. IRM has been applied qualitatively to a large number of cell types, and it seems that there are two universal types of adhesion. Focal contacts are small regions of closest cell-substrate apposition, possibly of immediate contact, that are associated with the distal end of actin filament bundles. They are firm attachment structures that hold the cell in place and in its spread shape. Close contacts are broad areas of reduced cell-to-substrate distance. They are weaker but highly dynamic adhesions that sustain rapid movements of cells or cell parts over the substrate. Although a number of independent observations suggest that adhesion patterns of malignantly transformed cells differ from those of their normal counterparts, there is no simple correlation between malignancy in vivo and altered contact formation in vitro. The adhesion pattern seems to be determined by the locomotory state of the cells rather than by their tissue of origin. Finally, IRM can also be used to enhance contrast in images of fixed preparations.
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Valavanis, Dimitrios, Paolo Ciocci, Gabriel N. Meloni, Peter Morris, Jean-François Lemineur, Ian J. McPherson, Frédéric Kanoufi, and Patrick R. Unwin. "Hybrid scanning electrochemical cell microscopy-interference reflection microscopy (SECCM-IRM): tracking phase formation on surfaces in small volumes." Faraday Discussions 233 (2022): 122–48. http://dx.doi.org/10.1039/d1fd00063b.

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Valavanis, Dimitrios, Paolo Ciocci, Gabriel N. Meloni, Peter Morris, Jean-François Lemineur, Ian J. McPherson, Frédéric Kanoufi, and Patrick R. Unwin. "Hybrid scanning electrochemical cell microscopy-interference reflection microscopy (SECCM-IRM): tracking phase formation on surfaces in small volumes." Faraday Discussions 233 (2022): 122–48. http://dx.doi.org/10.1039/d1fd00063b.

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Zand, M. S., and G. Albrecht-Buehler. "Long-term observation of cultured cells by interference-reflection microscopy: near infrared illumination and Y-contrast image processing." Proceedings, annual meeting, Electron Microscopy Society of America 46 (1988): 70–71. http://dx.doi.org/10.1017/s0424820100102432.

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Analysis of dynamic changes in cell-substratum adhesion patterns during cell locomotion requires continuous, extended observation of single living cells. To date, interference-reflection microscopy (IRM) is the only method available to visualize cell -substratum adhesions in vitro. This method uses 1% of the incident illumination to produce an IRM image, and so far requires use of a high intensity visible light source (400 - 800 nm). However, light of this intensity and spectral range induces marked changes in fibroblast behavior, including cessation of locomotion. Therefore, we developed a method allowing continuous IRM observation of live cells for up to 8 hours, without any observable changes in normal cell behavior, using near infrared illumination (750-1100 nm). In addition, we use Y-contrast image processing of IRM images to create a 3-dimensional relief of the ventral cell surface.Single locomoting PTK1 cells were observed continuously in IRM with time lapse video recording for periods of up to 8 hours.
5

Lai, Quintin J., Stuart L. Cooper, and Ralph M. Albrecht. "Thrombus formation on artificial surfaces: Correlative microscopy." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 3 (August 12, 1990): 840–41. http://dx.doi.org/10.1017/s042482010016176x.

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Thrombus formation and embolization are significant problems for blood-contacting biomedical devices. Two major components of thrombi are blood platelets and the plasma protein, fibrinogen. Previous studies have examined interactions of platelets with polymer surfaces, fibrinogen with platelets, and platelets in suspension with spreading platelets attached to surfaces. Correlative microscopic techniques permit light microscopic observations of labeled living platelets, under static or flow conditions, followed by the observation of identical platelets by electron microscopy. Videoenhanced, differential interference contrast (DIC) light microscopy permits high-resolution, real-time imaging of live platelets and their interactions with surfaces. Interference reflection microscopy (IRM) provides information on the focal adhesion of platelets on surfaces. High voltage, transmission electron microscopy (HVEM) allows observation of platelet cytoskeletal structure of whole mount preparations. Low-voltage, high resolution, scanning electron microscopy allows observation of fine surface detail of platelets. Colloidal gold-labeled fibrinogen, used to identify the Gp Ilb/IIIa membrane receptor for fibrinogen, can be detected in all the above microscopies.
6

Todd, I., J. S. Mellor, and D. Gingell. "Mapping cell-glass contacts of Dictyostelium amoebae by total internal reflection aqueous fluorescence overcomes a basic ambiguity of interference reflection microscopy." Journal of Cell Science 89, no. 1 (January 1, 1988): 107–14. http://dx.doi.org/10.1242/jcs.89.1.107.

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The widespread ability of eukaryotic cells to produce thin cytoplasmic sheets or lamellae 100–200 nm thick can give rise to uncertainties in the interpretation of interference reflection microscopy (IRM) images when cell-substratum topography is the key interest. If allowed to spread upon a poly-L-lysine-coated surface, Dictyostelium discoideum amoebae typically form ultrathin lamellae of approximately equal to 100 nm thickness by cytoplasmic retraction. Whereas the cell body is grey, the lamellae appear very dark under IRM optics. These dark areas could be misinterpreted as stemming from a closer cell-substratum apposition beneath the lamellae than the cell body. This ambiguity can be avoided if the technique of total internal reflection aqueous fluorescence (TIRAF) is used in conjunction with a high refractive index glass (n = 1.83) as substratum. Contributions to the image generated by thin cytoplasm and also variable cytoplasmic refractive index are thereby minimized due to the extremely short range of the ‘illuminating’ evanescent wave. From our comparative IRM and TIRAF study of the ultrathin lamellae of Dictyostelium amoebae it is concluded that the cell-glass gap is relatively uniform beneath the entire cell. We briefly discuss the sensitivity of several cell types to TIRAF, the generation of ultrathin lamellae and the nature of the cell-glass gap.
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Richter, Ekkehard, Hermine Hitzler, Heiko Zimmermann, Rolf Hagedorn, and G�nter Fuhr. "Trace formation during locomotion of L929 mouse fibroblasts continuously recorded by interference reflection microscopy (IRM)." Cell Motility and the Cytoskeleton 47, no. 1 (2000): 38–47. http://dx.doi.org/10.1002/1097-0169(200009)47:1<38::aid-cm4>3.0.co;2-w.

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Singer, I. I., D. M. Kazazis, and S. Scott. "Scanning electron microscopy of focal contacts on the substratum attachment surface of fibroblasts adherent to fibronectin." Journal of Cell Science 93, no. 1 (May 1, 1989): 147–54. http://dx.doi.org/10.1242/jcs.93.1.147.

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We have examined the cell-to-substratum attachment surface of hamster fibroblasts with scanning EM, and describe the surface ultrastructure of focal contacts and microspikes during cellular attachment and spreading on fibronectin. Nil 8 fibroblasts were seeded onto fibronectin-coated glass coverslips in serum-free medium, fixed, and the fibroblast-fibronectin monolayer was separated from the glass and inverted for scanning electron microscopic (EM) analysis. Focal contact development was detected by interference reflection microscopy and correlated with the immunofluorescence microscopic distribution of fibronectin receptor antigens. The cell undersurface appeared smooth and featureless at 0.5 h when focal contacts were undetectable and fibronectin receptors were distributed diffusely. By 1–2 h, undersurface membrane impressions of focal contacts were detected with scanning EM; their size, shape and distribution matched that of focal contacts seen with interference reflection microscopy (IRM). These contacts had smooth external surfaces and were often arranged in chevron-shaped complexes. However, at 4–6 h, the surface texture of focal contacts became fibrous and the contact periphery was delineated with the orifices of membrane-associated vesicles. Development of this filamentous substructure is correlated with the maximum concentration of fibronectin receptors and fibronectin at focal contacts, suggesting that these molecules are involved in the maturation and stabilization of focal contacts.
9

Paddock, S. W. "Tandem scanning reflected-light microscopy of cell-substratum adhesions and stress fibres in Swiss 3T3 cells." Journal of Cell Science 93, no. 1 (May 1, 1989): 143–46. http://dx.doi.org/10.1242/jcs.93.1.143.

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This paper describes two applications of the tandem scanning reflected-light microscope (TSM) for the observation of the structure of individual cells growing in tissue culture. First, the TSM is used as an alternative to interference reflection microscopy (IRM) or total internal reflection aqueous fluorescence microscopy (TIRAF) to observe cell-substratum adhesions in unstained living cells growing on a glass coverslip. Second, the TSM is used to produce improved images of cellular structures in 3T3 cells stained with various protein dyes including Napthol Blue Black (NBB) and Coomassie Brilliant Blue (CBB). More specifically, close contacts and focal contacts are resolved in living 3T3 cells, and features of the nucleus, the cytoskeleton and extracellular matrix are resolved in both NBB- and CBB-stained cells. The focal contacts and associated stress fibres are clearly imaged in NBB-stained cells. The TSM is an improvement over conventional incident light microscopy because of the confocal image excludes information from out-of-focus regions of the cytoplasm, and, unlike the laser-based confocal microscope, the actual colour of the specimen is viewed directly with TSM in almost real-time.
10

Izzard, C. S. "Optical studies on the development of the focal contact." Proceedings, annual meeting, Electron Microscopy Society of America 46 (1988): 120–21. http://dx.doi.org/10.1017/s0424820100102687.

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The focal contact is a localized region of relatively strong adhesion formed between cultured fibroblasts and planar substrates. The contact can be visualized and its formation followed in the live cell by the use of high illuminating-numerical-aperture interference reflection microscopy (IRM). The focal contact is the site into which stress fibers, or bundles of microfilaments, insert at the plasma membrane via a patch of amorphous material, the adhesion plaque. Through the use of immunochemical staining, a number of proteins have been shown to be concentrated at the focal contact/adhesion plaque complex. However, little is known about the sequence of events at the structural and protein levels that result in formation of the focal contact/adhesion plaque complex. We demonstrated previously that the focal contact forms rapidly as a stable adhesion beneath the motile lamellipodium at the cell margin. Parallel IRM and differential interference contrast (DIC)observations further showed that the contact forms specifically beneath a rib-like structure in the lamellipodium, which therefore functions as a precursor of the focal contact.
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Journal articles

Dissertations / Theses on the topic "Interference reflection microscopy (IRM)":

1

Ullberg, Nathan. "Visibility and charge density imaging of 2-dimensional semiconductors and devices studied using optical microscopy techniques IRM and BALM." Electronic Thesis or Diss., université Paris-Saclay, 2023. http://www.theses.fr/2023UPAST219.

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La microscopie optique a joué un rôle déterminant dans la recherche sur les matériaux bidimensionnels (2D). En particulier, les phénomènes d'interférences dans des couches minces ont été exploités pour améliorer le contraste et la résolution verticale lors de l'observation des matériaux 2D et ce jusqu'à l'échelle sub-nanométrique, souvent par l'intermédiaire de résonateurs Fabry-Pérot (FP). Dans cette thèse, la microscopie IRM (interference reflection microscopy) et la microscopie BALM (backside absorbing layer microscopy), qui abritent tous deux des effets FP, sont développées et utilisées pour étudier la visibilité et les inhomogénéités topographiques du MoS₂ bidimensionnel. Les données expérimentales de contraste sont comparées à des simulations. Pour l'IRM, une configuration optimale est proposée en ajustant la longueur d'onde incidente et l'indice de réfraction du milieu supérieur, ce qui permet d'obtenir un contraste de ≈ 80%. Pour la technique BALM, les propriétés optiques sont mesurées à la fois pour la couche absorbante antireflet de Cr/Au nanométrique et pour une couche isolante supplémentaire d'AlOₓ. Pour la première fois, le spectre de contraste de ce système a été mesuré et simulé, ce qui a permis d'obtenir un contraste expérimental maximal de ≈ 79% pour le MoS₂ 2D. Des simulations supplémentaires de l'empilement optique sur une gamme variable d'ouvertures du diaphragme et d'épaisseurs des couches FP prévoient une optimisation possible des conditions BALM pour un contraste encore augmenté. D'autres paramètres ont également été pris en compte, notamment la focalisation en z, le bruit dû aux chemins optiques parasites, les traitements d'image, etc. En s'appuyant sur ces études de contraste, une technique permettant d'imager la densité de charge dans le MoS₂ 2D et d'autres cristaux de dichalcogénures de métaux de transition a été développée. Celle-ci exploite la dépendance de l'indice de réfraction complexe en fonction de la charge aux longueurs d'onde proches de celles des excitons. Des condensateurs et des transistors à effet de champ (FET) en MoS₂ ont été fabriqués et de multiples expériences in operando ont été réalisées. En mode IRM, une grille électrolytique a été utilisée. Cela a permis de visualiser les délais et les inhomogénéités de chargement dus aux résistances intra- et inter-feuillets du MoS₂ polycristallin. Pour les transistors à effet de champ en MoS₂ (de type Schottky) la compétition entre les tensions de drain et de grille pour le contrôle de la densité de charge locale dans le canal a été étudiée pour la première fois par microscopie optique. Des condensateurs en MoS₂ à l'état solide intégrés avec l'empilement antireflet en conditions BALM sont également présentés pour la première fois, expérimentalement et au travers de simulations. Un dispositif transistor à l'état solide préliminaire a enfin été réalisé, illustrant les mérites de combiner à l'avenir l'imagerie de charge et les mesures électriques. Ce travail sur les aspects de contraste amélioré et d'imagerie de charge vise à élargir le rôle et l'impact des techniques de microscopie optique dans le domaine des matériaux 2D
Optical microscopy has played an instrumental role in 2-dimensional (2D) materials research. In particular, the phenomenon of thin-film interference of light has been leveraged to improve contrast and vertical resolution of 2D materials down to the sub-nanometer scale, often via Fabry-Pérot (FP) thin-film resonators. In this thesis, interference reflection microscopy (IRM) and backside absorbing layer microscopy (BALM), both of which harbor FP effects, are developed and utilized to study visibility and topographic inhomogeneities of the 2D semiconductor MoS₂. Experimental contrast data are compared against Fresnel-based simulations of contrast. For IRM, an optimal configuration was found by tuning of incident wavelength and top medium refractive index, yielding ≈ 80% contrast. For BALM, the optical properties were measured for both the anti-reflective absorbing layer of nanometric Cr/Au, and an additional insulating AlOₓ layer, where for the first time the contrast spectrum for this system was acquired and simulated, yielding a maximum experimental contrast of ≈ 79% for 2D MoS₂. Simulations of the optical stack across a variable range of aperture stop diameters and FP layer thicknesses predict further improvement of BALM conditions for high-contrast MoS₂ visibility. Additional aspects including z-focus, optical noise, image post-processing, and others were also considered. Building on the visibility aspects, a charge density imaging capability for 2D MoS₂ and other transition metal dichalcogenide crystals was developed by leveraging the charge-dependent complex refractive index near the wavelengths of the excitons. Capacitors and field-effect transistors (FET) of MoS₂ were realized, with multiple in operando experiments performed in widefield at throughputs up to 4 fps. In IRM mode, a liquid electrolyte gate was used, where charging delays and inhomogeneities due to intra- and inter-flake resistances in polycrystalline MoS₂ are presented. For Schottky barrier MoS₂ FETs, the drain versus gate voltage competition for control of the local charge density in the channel was studied for the first time by optical microscopy. Solid-state MoS₂ capacitor devices integrated in a BALM optical stack are also presented for the first time, both by experiments and simulations. A preliminary solid-state FET device was realized, exemplifying the powerful idea of combining optical charge imaging with electrical characterization in tandem. This work on visibility and charge imaging aspects aims to widen the role and impact of optical microscopy techniques in the space of 2D materials research
2

Simmert, Steve, and Erik Schäffer. "Interference reflection microscopy to visualize sub-diffraction limited objects in 3D." Diffusion fundamentals 20 (2013) 75, S. 1, 2013. https://ul.qucosa.de/id/qucosa%3A13662.

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Simmert, Steve, and Erik Schäffer. "Interference reflection microscopy to visualize sub-diffraction limited objects in 3D." Universitätsbibliothek Leipzig, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-183633.

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Simmert, Steve [Verfasser], and Erik [Akademischer Betreuer] Schäffer. "Optical tweezers combined with interference reflection microscopy for quantitative trapping and 3D imaging / Steve Simmert ; Betreuer: Erik Schäffer." Tübingen : Universitätsbibliothek Tübingen, 2018. http://d-nb.info/1199268771/34.

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Book chapters on the topic "Interference reflection microscopy (IRM)":

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Kihm, Kenneth D. "Reflection Interference Contrast Microscopy (RICM)." In Near-Field Characterization of Micro/Nano-Scaled Fluid Flows, 119–30. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-20426-5_6.

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Rädler, J., and E. Sackmann. "Vesicle-Substrate Interaction Studied by Reflection Interference Contrast Microscopy." In Springer Proceedings in Physics, 158–61. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-84763-9_30.

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Curtis, A. S. G. "Interference Reflection Microscopy and Related Microscopies and Cell Adhesion." In Studying Cell Adhesion, 185–93. Berlin, Heidelberg: Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-662-03008-0_13.

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Abdelrahman, Ahmed, Ana-Sunčana Smith, and Kheya Sengupta. "Observing Membrane and Cell Adhesion via Reflection Interference Contrast Microscopy." In The Immune Synapse, 123–35. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3135-5_8.

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Schürch, S., F. Green, M. Schoel, and P. Gehr. "Adhesion of Pulmonary Macrophages to Langmuir-Blodgett Films, Investigated by Interference Reflection Microscopy." In Springer Series in Biophysics, 244–45. Berlin, Heidelberg: Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-73925-5_44.

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Fletcher, Madilyn. "The Application of Interference Reflection Microscopy to the Study of Bacterial Adhesion to Solid Surfaces." In Biodeterioration 7, 31–35. Dordrecht: Springer Netherlands, 1988. http://dx.doi.org/10.1007/978-94-009-1363-9_4.

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"IRM (interference-reflection microscopy)." In Encyclopedia of Genetics, Genomics, Proteomics and Informatics, 1035. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6754-9_8753.

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Ploem, J. S., F. A. Prins, and I. Cornelese-Ten Velde. "Reflection-contrast microscopy." In Light Microscopy in Biology, 275–310. Oxford University PressOxford, 1999. http://dx.doi.org/10.1093/oso/9780199636709.003.0007.

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Abstract Fauré-Fremiet (1) used reflected light in cell biology studies half a century ago. Curtis (2) and Izzard and Lochner (3) performed the first fundamental studies of interference images of living cells on glass surfaces using reflected light microscopy. Reflection images were obtained with scanning reflecting microscopy (4, 5). Reflected-light microscopy for the study of living cells (2, 3, 6, 7) has been described as interference reflection, reflection-interference contrast, surface-contrast, and surface-:reflection interference microscopy (8). Ploem (9, 10) investigated further optical methods to improve the image contrast in reflected-light microscopy. In collaboration with Leica (11-13), an improved microscope system was developed for reflected-light microscopy. It used an aperture diaphragm with a central stop (creating an annular aperture) in the epi-illumination light path at an aperture plane conjugate with the back focal (aperture) plane of the objective (14). This was combined with epipolarization microscopy using immersion objectives equipped with a quarter lambda plate in their front lens (15). New high aperture objectives were developed for high resolution biological microscopy. This optically improved version of reflected-light microscopy was named reflection-contrast micro scopy (RCM).
9

Weber, Igor. "[2] Reflection interference contrast microscopy." In Methods in Enzymology, 34–47. Elsevier, 2003. http://dx.doi.org/10.1016/s0076-6879(03)61004-9.

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Conference papers on the topic "Interference reflection microscopy (IRM)":

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Opas, Michal, and Michal Opas. "Biomedical Applications Of Interference Reflection Microscopy." In Interferometry '89, edited by Zbigniew Jaroszewicz, Maksymilian Pluta, Zbigniew Jaroszewicz, and Maksymilian Pluta. SPIE, 1990. http://dx.doi.org/10.1117/12.961294.

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Davies, Heather S., Natalia S. Baranova, Nouha El Amri, Liliane Coche-Guérente, Claude Verdier, Lionel Bureau, Ralf P. Richter, and Delphine Débarre. "Blood cell - vessel wall interactions probed by reflection interference contrast microscopy." In Advances in Microscopic Imaging, edited by Francesco S. Pavone, Emmanuel Beaurepaire, and Peter T. So. SPIE, 2019. http://dx.doi.org/10.1117/12.2527058.

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Lee, Byron S., and T. C. Strand. "Scanning Interference Microscopy for Surface Characterization." In Optical Fabrication and Testing. Washington, D.C.: Optica Publishing Group, 1988. http://dx.doi.org/10.1364/oft.1988.tha8.

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Recent work has introduced the concept of scanning interference microscopy which has 3-D resolution comparable to a confocal microscope (1). This is obtained by performing interference microscopy with spatially incoherent and broadband illumination. By scanning along the optical axis, one can measure the coherence function at each point in the image. This coherence function can be processed to obtain various pieces of information. The maximum of the envelope of the coherence function corresponds to the surface height. By Fourier processing of the fringes at each point, the spectral reflectivity can be measured for that point, determining both the magnitude and the phase of the reflection as a function of wavelength. Thus a tremendous amount of information is available, but at the cost of a high processing overhead. A PC based system which provides a low cost solution with good performance will be discussed, with trade-offs between range resolution, number of points processed, and precision versus the processing time and hardware requirements analyzed. Traditionally, interferometric techniques have not been applicable to optically rough surfaces or heterogenous surfaces. This talk will discuss how scanning interference microscopy can be applied to such surfaces.
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Kandel, Mikhail E., Catherine Best-Popescu, and Gabriel Popescu. "Reflection gradient light interference microscopy (epi-GLIM) for label-free imaging of bulk specimens (Conference Presentation)." In Quantitative Phase Imaging IV, edited by Gabriel Popescu and YongKeun Park. SPIE, 2018. http://dx.doi.org/10.1117/12.2294032.

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Lin, J. A., and W. T. Yeh. "A Grating Interferometer For Testing The Zone Plate." In Optical Fabrication and Testing. Washington, D.C.: Optica Publishing Group, 1988. http://dx.doi.org/10.1364/oft.1988.thb10.

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The zone plate is a diffraction optical element which images by interference, rather than by reflection or refraction. The applications of the zone plate include X-ray microscopy, focusing and coupling elements in opto-electronics, X-ray astronomy and etc. The focusing profile of the zone plate has been studied quite well. However, the wavefront aberration of the zone plate was less tested or evaluated by the interferometer1. We use a sinusoidal grating in the Ronchi interferometer to split the wavefront under test into three. The wavefront aberration for both amplitude and phase zone plate may be calculated by taking a few measurements from the three-beam interferograms. The accuracy of the test will be discussed. Since the grating is also a diffraction optical element, it may live together with the zone plate in the region of various radiations.
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Lee, Dooyoung, Karen P. Fong, Lawrence F. Brass, and Daniel A. Hammer. "Dynamic Spreading of Platelets on Collagen in Microchannels." In ASME 2009 7th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2009. http://dx.doi.org/10.1115/icnmm2009-82247.

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Platelets come contact collagen exposed on the subendothelial matrix at sites of vascular injury that triggers their activation and the formation of a hemostatic plug. Glycoprotein VI and integrin α2β1 are major collagen receptors on the platelet surface. Although the spreading of platelets on the collagen is important to function, it has been difficult to study the dynamics of spreading over a relevant time scale. Here we focus on the early stages of murine platelet spreading on collagen and/or fibrinogen under both static conditions and flow and then probe their dynamics by quantitative visualization using real-time reflection interference contrast microscopy. In this study, we found under static conditions the spreading area of platelets on collagen initially increased quickly by following the power law A ∼ t0.7 before slowing. Interestingly, we observed in microchannels under flow that single platelets as well as aggregates that were spread on the collagen contracted over time under shear. This contraction was not observed under static conditions in our system. This effect might help to maintain the hemostatic plug under the shear force. Future studies will be aimed at investigating the spatial-temporal cytoskeletal dynamics of platelets and the functions of collagen receptors on the platelets surface using platelets derived from genetically engineered mice.

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