Academic literature on the topic 'Integrity-PCR'
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Journal articles on the topic "Integrity-PCR"
Perkin, L. C., B. Oppert, S. Duke, and C. P.-C. Suh. "Assessment of DNA Integrity From Trap-Captured Boll Weevil (Coleoptera: Curculionidae) for Use in a New PCR-Based Diagnostic Tool." Journal of Economic Entomology 114, no. 3 (April 22, 2021): 1321–28. http://dx.doi.org/10.1093/jee/toab073.
Full textDu, Xiongwei, Hongjie An, Bo Jin, Liangyu Meng, and Qingdai Liu. "Carbon Nanotubes Altering Specificity of Repeated PCR and DNA Integrity Properties." Journal of Nanoscience and Nanotechnology 14, no. 7 (July 1, 2014): 5547–51. http://dx.doi.org/10.1166/jnn.2014.8874.
Full textBergerová, E., Z. Godálová, and P. Siekel. "Combined effects of temperature, pressure and low pH on the amplification of DNA of plant derived foods." Czech Journal of Food Sciences 29, No. 4 (August 10, 2011): 337–45. http://dx.doi.org/10.17221/217/2010-cjfs.
Full textFrasquilho, Sonia G., Ignacio Sanchez, Changyoung Yoo, Laurent Antunes, Camille Bellora, and William Mathieson. "Do Tissues Fixed in a Non-crosslinking Fixative Require a Dedicated Formalin-free Processor?" Journal of Histochemistry & Cytochemistry 69, no. 6 (May 19, 2021): 389–405. http://dx.doi.org/10.1369/00221554211017859.
Full textRaja, Siva, Jesus Ching, Liqiang Xi, Steven J. Hughes, Ronald Chang, Wendy Wong, William McMillan, et al. "Technology for Automated, Rapid, and Quantitative PCR or Reverse Transcription-PCR Clinical Testing." Clinical Chemistry 51, no. 5 (May 1, 2005): 882–90. http://dx.doi.org/10.1373/clinchem.2004.046474.
Full textFleige, Simone, and Michael W. Pfaffl. "RNA integrity and the effect on the real-time qRT-PCR performance." Molecular Aspects of Medicine 27, no. 2-3 (April 2006): 126–39. http://dx.doi.org/10.1016/j.mam.2005.12.003.
Full textGorzelak-Pabis, Paulina, Emilia Luczak, Katarzyna Wojdan, Karolina Antosik, Maciej Borowiec, Marlena Broncel, and Maciej Chalubinski. "Endothelial integrity may be regulated by a specific antigen via an IgE-mediated mechanism." Postępy Higieny i Medycyny Doświadczalnej 71, no. 1 (March 2, 2017): 0. http://dx.doi.org/10.5604/01.3001.0010.3800.
Full textOakey, H. Jane. "A Universal Test to Determine the Integrity of RNA, and its Suitability for Reverse Transcription, in Animal Tissue Laboratory Specimens." Journal of Veterinary Diagnostic Investigation 19, no. 5 (September 2007): 459–64. http://dx.doi.org/10.1177/104063870701900501.
Full textCédile, Oriane, Sólja Remisdóttir Veyhe, Marcus Høy Hansen, Kjell Titlestad, and Charlotte Guldborg Nyvold. "Investigation of circulating DNA integrity after blood collection." BioTechniques 71, no. 5 (November 2021): 550–55. http://dx.doi.org/10.2144/btn-2020-0167.
Full textDidelot, Audrey, Steve K. Kotsopoulos, Audrey Lupo, Deniz Pekin, Xinyu Li, Ivan Atochin, Preethi Srinivasan, et al. "Multiplex Picoliter-Droplet Digital PCR for Quantitative Assessment of DNA Integrity in Clinical Samples." Clinical Chemistry 59, no. 5 (May 1, 2013): 815–23. http://dx.doi.org/10.1373/clinchem.2012.193409.
Full textDissertations / Theses on the topic "Integrity-PCR"
Garlan, Fanny. "Nouvelles méthodes de détection de l'ADN tumoral circulant par PCR digitale en gouttelettes : application au suivi des patients." Thesis, Sorbonne Paris Cité, 2016. http://www.theses.fr/2016USPCB108/document.
Full textCirculating tumor DNA (ctDNA) carries tumor-specific alterations that are detectable by minimally invasive sampling. It represents a highly pertinent marker for cancer monitoring during patients’ follow-up. CtDNA detection requires a highly sensitive and quantitative technique. In this context, this project focused on ctDNA quantification and monitoring by picoliter-droplet digital PCR. Thanks to the compartmentalization in millions of picoliter droplets, this tool allowed the detection of single DNA molecule with a sensitivity reaching 0.001%. Testing of ctDNA was performed through the evaluation of different potential biomarkers: specific mutations, ctDNA fragmentation, and hypermethylation of target sequences. On one hand, we observed in cancer patients that ctDNA is more fragmented than wild-type DNA, and, globally more fragmented than circulating DNA in healthy individuals. On the other hand, a strong correlation between percentages of hypermethylated and mutated DNA was observed during the follow-up of patients. Such results suggest the feasibility to precisely and quantitatively monitor ctDNA by the evaluation of hypermethylation as an alternative to the determination of mutational status. We have applied such ctDNA detection strategies in the context of two clinical studies. The PLACOL study, enrolling 82 metastatic colorectal cancer patients, allowed to highlight two prognostic factors: a ctDNA concentration threshold of 0.1 ng / mL, and the evaluation of ctDNA decreasing slope. In the second study, ctDNA was monitored in 11 melanoma patients in the context of a targeted therapy (vemurafenib). An inverse correlation between the concentrations of vemurafenib and ctDNA was demonstrated. These results suggest the clinical relevancy of ctDNA in advanced cancer patients, for the optimization of therapeutic management
Normand, Camille. "La rhinοpneumοnie équine : caractérisatiοn mοléculaire et cellulaire de l'herpèsvirus équin 4, un mοdèle d'étude pοur l'appοrt de cοnnaissances dans la pathοgénie de la maladie, la survie et l'intégrité du virus ainsi que l'identificatiοn de mοlécules antivirales." Electronic Thesis or Diss., Normandie, 2024. http://www.theses.fr/2024NORMC405.
Full textUntil 1981, equine herpesvirus 4 (EHV-4) and EHV-1, which cause rhinopneumonitis, were considered to be two subtypes of the same virus. Since then, it has been shown that EHV-4 is only marginally involved in abortions, and its involvement in the nervous form of the disease has not yet been demonstrated. This is probably why this virus is less studied than HVE-1. This thesis focused on three important aspects of rhinopneumonitis in horses in order to gain a better understanding of EHV 4 and to assess the value of using this virus as a model. By comparing two epizootics at two stud farms with different vaccination profiles and measuring EHV-4 excretion and viremia by qPCR, we were able to demonstrate the benefits of vaccination and the appearance of seroconversions. Although contamination occurs mainly through contact, the impact of the virus's survival in the environment needs to be studied. We have shown that EHV-4 can survive for at least 28 days at 4 °C in water and on various surfaces. We have also developed an integrity-PCR method to differentiate between infectious and non-infectious viruses. Finally, we have screened a number of compounds using RTCA and demonstrated the efficacy of several of them, including decitabine, for which we have carried out a preliminary study of the mode of action using transcriptomic analysis
Book chapters on the topic "Integrity-PCR"
Pinz, Sophia, Eva Doskocil, and Wolfgang Seufert. "Thermofluor-Based Analysis of Protein Integrity and Ligand Interactions." In Ribosome Biogenesis, 247–57. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2501-9_15.
Full text"Trusted Cloud Computing." In Detection and Mitigation of Insider Attacks in a Cloud Infrastructure, 12–27. IGI Global, 2019. http://dx.doi.org/10.4018/978-1-5225-7924-3.ch002.
Full textEmbleton, M. Jim. "Reverse transcriptase in situ PCR for RNA detection." In PCR3, 87–102. Oxford University PressOxford, 1997. http://dx.doi.org/10.1093/oso/9780199636327.003.0005.
Full textKrzyściak, Wirginia, Marta Szwajca, Paulina Karcz, Aleksander Turek, Natalia Śmierciak, Amira Bryll, Paulina Mazur, et al. "Myo-inositol’s Role in Understanding the Pain Perception in Patients with Schizophrenia." In New Approaches to the Management and Diagnosis of Schizophrenia [Working Title]. IntechOpen, 2024. http://dx.doi.org/10.5772/intechopen.1005244.
Full textPanwar, Gautam, Ankur Vashishtha, Navdeep Singh, and Salender Singh. "BACTERIAL LABORATORY DIAGNOSIS OF CLINICAL SAMPLES." In COMPENDIUM OF MEDICAL DIAGNOSTIC TECHNOLOGY. NOBLE SCIENCE PRESS, 2023. http://dx.doi.org/10.52458/9789388996846.nsp2023.eb.ch-07.
Full textReports on the topic "Integrity-PCR"
Qamhia, Issam, Erol Tutumluer, and Han Wang. Aggregate Subgrade Improvements Using Quarry By-products: A Field Investigation. Illinois Center for Transportation, June 2021. http://dx.doi.org/10.36501/0197-9191/21-017.
Full textCahaner, Avigdor, Sacit F. Bilgili, Orna Halevy, Roger J. Lien, and Kellye S. Joiner. effects of enhanced hypertrophy, reduced oxygen supply and heat load on breast meat yield and quality in broilers. United States Department of Agriculture, November 2014. http://dx.doi.org/10.32747/2014.7699855.bard.
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