Relevant bibliographies by topics / Integrative conjugative element / Journal articles

Journal articles on the topic 'Integrative conjugative element'

To see the other types of publications on this topic, follow the link: Integrative conjugative element.
Author: Grafiati
Published: 10 December 2022
Last updated: 14 March 2023

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Integrative conjugative element.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Iannelli, Francesco, Francesco Santoro, Marco R. Oggioni, and Gianni Pozzi. "Nucleotide Sequence Analysis of Integrative Conjugative Element Tn5253of Streptococcus pneumoniae." Antimicrobial Agents and Chemotherapy 58, no. 2 (December 2, 2013): 1235–39. http://dx.doi.org/10.1128/aac.01764-13.

Full text
Abstract:
ABSTRACTConjugative transposon Tn5253, an integrative conjugative element (ICE) ofStreptococcus pneumoniaecarrying thecatandtet(M) genes, was shown to be 64,528 bp in size and to contain 79 open reading frames, of which only 38 could be annotated. Two distinct genetic elements were found integrated into Tn5253: Tn5251(18,033 bp), of the Tn916-Tn1545family of ICEs, and Ωcat(pC194) (7,627 bp), which could not conjugate but was capable of intracellular mobility by excision, circularization, and integration by homologous recombination. The highest conjugation frequency of Tn5253was observed whenStreptococcus pyogeneswas the donor (6.7 × 10−3transconjugants/donor).
APA, Harvard, Vancouver, ISO, and other styles
2

Ringwald, Kenneth, and Jeffrey Gardner. "The Bacteroides thetaiotaomicron Protein Bacteroides Host Factor A Participates in Integration of the Integrative Conjugative Element CTnDOT into the Chromosome." Journal of Bacteriology 197, no. 8 (February 2, 2015): 1339–49. http://dx.doi.org/10.1128/jb.02198-14.

Full text
Abstract:
ABSTRACTCTnDOT is a conjugative transposon found inBacteroidesspecies. It encodes multiple antibiotic resistances and is stimulated to transfer by exposure to tetracycline. CTnDOT integration into the host chromosome requires IntDOT and a previously unknown host factor. We have identified a protein, designated BHFa (Bacteroideshost factor A), that participates in integrative recombination. BHFa is the first host factor identified for a site-specific recombination reaction in the CTnDOT family of integrative and conjugative elements. Based on the amino acid sequence of BHFa, the ability to bind specifically to 4 sites in theattDOTDNA, and its activity in the integration reaction, BHFa is a member of the IHF/HU family of nucleoid-associated proteins. Other DNA bending proteins that bind DNA nonspecifically can substitute for BHFa in the integration reaction.IMPORTANCEBacteroidesspecies are normal members of the human colonic microbiota. These species can harbor and spread self-transmissible genetic elements (integrative conjugative elements [ICEs]) that contain antibiotic resistance genes. This work describes the role of a protein, BHFa, and its importance in the integration reaction required for the element CTnDOT to persist inBacteroideshost cells.
APA, Harvard, Vancouver, ISO, and other styles
3

Lee, Catherine A., and Alan D. Grossman. "Identification of the Origin of Transfer (oriT) and DNA Relaxase Required for Conjugation of the Integrative and Conjugative Element ICEBs1 of Bacillus subtilis." Journal of Bacteriology 189, no. 20 (August 10, 2007): 7254–61. http://dx.doi.org/10.1128/jb.00932-07.

Full text
Abstract:
ABSTRACT Integrative and conjugative elements (ICEs), also known as conjugative transposons, are mobile genetic elements that can transfer from one bacterial cell to another by conjugation. ICEBs1 is integrated into the trnS-leu2 gene of Bacillus subtilis and is regulated by the SOS response and the RapI-PhrI cell-cell peptide signaling system. When B. subtilis senses DNA damage or high concentrations of potential mating partners that lack the element, ICEBs1 excises from the chromosome and can transfer to recipients. Bacterial conjugation usually requires a DNA relaxase that nicks an origin of transfer (oriT) on the conjugative element and initiates the 5′-to-3′ transfer of one strand of the element into recipient cells. The ICEBs1 ydcR (nicK) gene product is homologous to the pT181 family of plasmid DNA relaxases. We found that transfer of ICEBs1 requires nicK and identified a cis-acting oriT that is also required for transfer. Expression of nicK leads to nicking of ICEBs1 between a GC-rich inverted repeat in oriT, and NicK was the only ICEBs1 gene product needed for nicking. NicK likely mediates conjugation of ICEBs1 by nicking at oriT and facilitating the translocation of a single strand of ICEBs1 DNA through a transmembrane conjugation pore.
APA, Harvard, Vancouver, ISO, and other styles
4

Burrus, Vincent, Yvonne Roussel, Bernard Decaris, and Gérard Guédon. "Characterization of a Novel Integrative Element, ICESt1, in the Lactic Acid BacteriumStreptococcus thermophilus." Applied and Environmental Microbiology 66, no. 4 (April 1, 2000): 1749–53. http://dx.doi.org/10.1128/aem.66.4.1749-1753.2000.

Full text
Abstract:
ABSTRACT The 35.5-kb ICESt1 element of Streptococcus thermophilus CNRZ368 is bordered by a 27-bp repeat and integrated into the 3′ end of a gene encoding a putative fructose-1,6-biphosphate aldolase. This element encodes site-specific integrase and excisionase enzymes related to those of conjugative transposons Tn5276and Tn5252. The integrase was found to be involved in a site-specific excision of a circular form. ICESt1 also encodes putative conjugative transfer proteins related to those of the conjugative transposon Tn916. Therefore, ICESt1could be or could be derived from an integrative conjugative element.
APA, Harvard, Vancouver, ISO, and other styles
5

Belhocine, Kamila, Karen K. Yam, and Benoit Cousineau. "Conjugative Transfer of the Lactococcus lactis Chromosomal Sex Factor Promotes Dissemination of the Ll.LtrB Group II Intron." Journal of Bacteriology 187, no. 3 (February 1, 2005): 930–39. http://dx.doi.org/10.1128/jb.187.3.930-939.2005.

Full text
Abstract:
ABSTRACT The Ll.LtrB group II intron from the low-G+C gram-positive bacterium Lactococcus lactis was the first bacterial group II intron shown to splice and mobilize in vivo. This retroelement interrupts the relaxase gene (ltrB) of three L. lactis conjugative elements: plasmids pRS01 and pAH90 and the chromosomal sex factor. Conjugative transfer of a plasmid harboring a segment of the pRS01 conjugative plasmid including the Ll.LtrB intron allows dissemination of Ll.LtrB among L. lactis strains and lateral transfer of this retroelement from L. lactis to Enterococcus faecalis. Here we report the dissemination of the Ll.LtrB group II intron among L. lactis strains following conjugative transfer of the native chromosomally embedded L. lactis sex factor. We demonstrated that Ll.LtrB dissemination is highly variable and often more efficient from this integrative and conjugative element than from an engineered conjugative plasmid. Cotransfer among L. lactis strains of both Ll.LtrB-containing elements, the conjugative plasmid and the sex factor, was detected and shown to be synergistic. Moreover, following their concurrent transfer, both mobilizable elements supported the spread of their respective copies of the Ll.LtrB intron. Our findings explain the unusually high efficiency of Ll.LtrB mobility observed following conjugation of intron-containing plasmids.
APA, Harvard, Vancouver, ISO, and other styles
6

Berkmen, Melanie B., Catherine A. Lee, Emma-Kate Loveday, and Alan D. Grossman. "Polar Positioning of a Conjugation Protein from the Integrative and Conjugative Element ICEBs1 of Bacillus subtilis." Journal of Bacteriology 192, no. 1 (September 4, 2009): 38–45. http://dx.doi.org/10.1128/jb.00860-09.

Full text
Abstract:
ABSTRACT ICEBs1 is an integrative and conjugative element found in the chromosome of Bacillus subtilis. ICEBs1 encodes functions needed for its excision and transfer to recipient cells. We found that the ICEBs1 gene conE (formerly yddE) is required for conjugation and that conjugative transfer of ICEBs1 requires a conserved ATPase motif of ConE. ConE belongs to the HerA/FtsK superfamily of ATPases, which includes the well-characterized proteins FtsK, SpoIIIE, VirB4, and VirD4. We found that a ConE-GFP (green fluorescent protein) fusion associated with the membrane predominantly at the cell poles in ICEBs1 donor cells. At least one ICEBs1 product likely interacts with ConE to target it to the membrane and cell poles, as ConE-GFP was dispersed throughout the cytoplasm in a strain lacking ICEBs1. We also visualized the subcellular location of ICEBs1. When integrated in the chromosome, ICEBs1 was located near midcell along the length of the cell, a position characteristic of that chromosomal region. Following excision, ICEBs1 was more frequently found near a cell pole. Excision of ICEBs1 also caused altered positioning of at least one component of the replisome. Taken together, our findings indicate that ConE is a critical component of the ICEBs1 conjugation machinery, that conjugative transfer of ICEBs1 from B. subtilis likely initiates at a donor cell pole, and that ICEBs1 affects the subcellular position of the replisome.
APA, Harvard, Vancouver, ISO, and other styles
7

Smyth, Davida S., and D. Ashley Robinson. "Integrative and Sequence Characteristics of a Novel Genetic Element, ICE6013, in Staphylococcus aureus." Journal of Bacteriology 191, no. 19 (July 31, 2009): 5964–75. http://dx.doi.org/10.1128/jb.00352-09.

Full text
Abstract:
ABSTRACT A survey of chromosomal variation in the ST239 clonal group of methicillin-resistant Staphylococcus aureus (MRSA) revealed a novel genetic element, ICE6013. The element is 13,354 bp in length, excluding a 6,551-bp Tn552 insertion. ICE6013 is flanked by 3-bp direct repeats and is demarcated by 8-bp imperfect inverted repeats. The element was present in 6 of 15 genome-sequenced S. aureus strains, and it was detected using genetic markers in 19 of 44 diverse MRSA and methicillin-susceptible strains and in all 111 ST239 strains tested. Low integration site specificity was discerned. Multiple chromosomal copies and the presence of extrachromosomal circular forms of ICE6013 were detected in various strains. The circular forms included 3-bp coupling sequences, located between the 8-bp ends of the element, that corresponded to the 3-bp direct repeats flanking the chromosomal forms. ICE6013 is predicted to encode 15 open reading frames, including an IS30-like DDE transposase in place of a Tyr/Ser recombinase and homologs of gram-positive bacterial conjugation components. Further sequence analyses indicated that ICE6013 is more closely related to ICEBs1 from Bacillus subtilis than to the only other potential integrative conjugative element known from S. aureus, Tn5801. Evidence of recombination between ICE6013 elements is also presented. In summary, ICE6013 is the first member of a new family of active, integrative genetic elements that are widely dispersed within S. aureus strains.
APA, Harvard, Vancouver, ISO, and other styles
8

Heather, Zoe, Matthew T. G. Holden, Karen F. Steward, Julian Parkhill, Lijiang Song, Gregory L. Challis, Carl Robinson, Nicholas Davis-Poynter, and Andrew S. Waller. "A novel streptococcal integrative conjugative element involved in iron acquisition." Molecular Microbiology 70, no. 5 (December 2008): 1274–92. http://dx.doi.org/10.1111/j.1365-2958.2008.06481.x.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Taviani, Elisa, Christopher J. Grim, Jongsik Chun, Anwar Huq, and R. R. Colwell. "Genomic analysis of a novel integrative conjugative element inVibrio cholerae." FEBS Letters 583, no. 22 (October 20, 2009): 3630–36. http://dx.doi.org/10.1016/j.febslet.2009.10.041.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Del Grosso, Maria, Romina Camilli, Ermanno Rizzi, Alessandro Pietrelli, Gianluca De Bellis, and Annalisa Pantosti. "ICESpy009, a Conjugative Genetic Element Carryingmef(E) in Streptococcus pyogenes." Antimicrobial Agents and Chemotherapy 60, no. 7 (April 11, 2016): 3906–12. http://dx.doi.org/10.1128/aac.03082-15.

Full text
Abstract:
ABSTRACTEfflux-mediated macrolide resistance due tomef(E) andmel, carried by the mega element, is common inStreptococcus pneumoniae, for which it was originally characterized, but it is rare inStreptococcus pyogenes. InS. pyogenes, mega was previously found to be enclosed in Tn2009, a composite genetic element of the Tn916family containingtet(M) and conferring erythromycin and tetracycline resistance. In this study,S. pyogenesisolates containingmef(E), apparently not associated with other resistance determinants, were examined to characterize the genetic context of mega. By whole-genome sequencing of one isolate, MB56Spyo009, we identified a novel composite integrative and conjugative element (ICE) carrying mega, designated ICESpy009, belonging to the ICESa2603 family. ICESpy009 was 55 kb long, contained 61 putative open reading frames (ORFs), and was found to be integrated intohylA, a novel integration site for the ICESa2603 family. The modular organization of the ICE was similar to that of members of the ICESa2603 family carried by different streptococcal species. In addition, a novel cluster of accessory resistance genes was found inside a region that encloses mega. PCR mapping targeting ICESpy009 revealed the presence of a similar ICE in five other isolates under study. While in three isolates the integration site was the same as that of ICESpy009, in two isolates the ICE was integrated intorplL, the typical integration site of the ICESa2603 family. ICESpy009 was able to transfer macrolide resistance by conjugation to bothS. pyogenesandS. pneumoniae, showing the first evidence of the transferability of mega fromS. pyogenes.
APA, Harvard, Vancouver, ISO, and other styles
11

Libante, Virginie, Nazim Sarica, Abbas Mohamad Ali, Chloé Gapp, Anissa Oussalah, Gérard Guédon, Nathalie Leblond-Bourget, and Sophie Payot. "Mobilization of IMEs Integrated in the oriT of ICEs Involves Their Own Relaxase Belonging to the Rep-Trans Family of Proteins." Genes 11, no. 9 (August 26, 2020): 1004. http://dx.doi.org/10.3390/genes11091004.

Full text
Abstract:
Integrative mobilizable elements (IMEs) are widespread but very poorly studied integrated elements that can excise and hijack the transfer apparatus of co-resident conjugative elements to promote their own spreading. Sixty-four putative IMEs, harboring closely related mobilization and recombination modules, were found in 14 Streptococcus species and in Staphylococcus aureus. Fifty-three are integrated into the origin of transfer (oriT) of a host integrative conjugative element (ICE), encoding a MobT relaxase and belonging to three distant families: ICESt3, Tn916, and ICE6013. The others are integrated into an unrelated IME or in chromosomal sites. After labeling by an antibiotic resistance gene, the conjugative transfer of one of these IMEs (named IME_oriTs) and its host ICE was measured. Although the IME is integrated in an ICE, it does not transfer as a part of the host ICE (no cis-mobilization). The IME excises and transfers separately from the ICE (without impacting its transfer rate) using its own relaxase, distantly related to all known MobT relaxases, and integrates in the oriT of the ICE after transfer. Overall, IME_oriTs use MobT-encoding ICEs both as hosts and as helpers for conjugative transfer. As half of them carry lsa(C), they actively participate in the dissemination of lincosamide–streptogramin A–pleuromutilin resistance among Firmicutes.
APA, Harvard, Vancouver, ISO, and other styles
12

She, Q., B. Shen, and L. Chen. "Archaeal integrases and mechanisms of gene capture." Biochemical Society Transactions 32, no. 2 (April 1, 2004): 222–26. http://dx.doi.org/10.1042/bst0320222.

Full text
Abstract:
Archaeal integrases facilitate the formation of two distinctive types of integrated element within archaeal chromosomes: the SSV type and pNOB8 type. The former carries a smaller N-terminal and a larger C-terminal integrase gene fragment, and the latter an intact integrase gene. All integrated elements overlap tRNA genes that were target sites for integration. It has been demonstrated that SSV (Sulfolobus spindle virus) viruses, carrying an SSV-type integrase gene, and conjugative plasmids, carrying a pNOB8-type integrase, are integrative elements. Two mechanisms have been proposed for stably maintaining an integrated element within archaeal chromosomes. There is also evidence for changes having occurred in the captured integrated elements present in archaeal genomes. Thus we infer that site-specific integration constitutes an important mechanism for horizontal gene transfer and genome evolution.
APA, Harvard, Vancouver, ISO, and other styles
13

Roy, David, Kevin T. Huguet, Frédéric Grenier, and Vincent Burrus. "IncC conjugative plasmids and SXT/R391 elements repair double-strand breaks caused by CRISPR–Cas during conjugation." Nucleic Acids Research 48, no. 16 (June 18, 2020): 8815–27. http://dx.doi.org/10.1093/nar/gkaa518.

Full text
Abstract:
Abstract Bacteria have evolved defence mechanisms against bacteriophages. Restriction-modification systems provide innate immunity by degrading invading DNAs that lack proper methylation. CRISPR–Cas systems provide adaptive immunity by sampling the genome of past invaders and cutting the DNA of closely related DNA molecules. These barriers also restrict horizontal gene transfer mediated by conjugative plasmids. IncC conjugative plasmids are important contributors to the global dissemination of multidrug resistance among pathogenic bacteria infecting animals and humans. Here, we show that IncC conjugative plasmids are highly resilient to host defence systems during entry into a new host by conjugation. Using a TnSeq strategy, we uncover a conserved operon containing five genes (vcrx089–vcrx093) that confer a novel host defence evasion (hde) phenotype. We show that vcrx089–vcrx090 promote resistance against type I restriction-modification, whereas vcrx091–vcxr093 promote CRISPR–Cas evasion by repairing double-strand DNA breaks via recombination between short sequence repeats. vcrx091, vcrx092 and vcrx093 encode a single-strand binding protein, and a single-strand annealing recombinase and double-strand exonuclease related to Redβ and λExo of bacteriophage λ, respectively. Homologous genes of the integrative and conjugative element R391 also provide CRISPR–Cas evasion. Hence, the conserved hde operon considerably broadens the host range of large families of mobile elements spreading multidrug resistance.
APA, Harvard, Vancouver, ISO, and other styles
14

Roy Chowdhury, Piklu, Martin Scott, Paul Worden, Peter Huntington, Bernard Hudson, Thomas Karagiannis, Ian G. Charles, and Steven P. Djordjevic. "Genomic islands 1 and 2 play key roles in the evolution of extensively drug-resistant ST235 isolates of Pseudomonas aeruginosa." Open Biology 6, no. 3 (March 2016): 150175. http://dx.doi.org/10.1098/rsob.150175.

Full text
Abstract:
Pseudomonas aeruginosa are noscomially acquired, opportunistic pathogens that pose a major threat to the health of burns patients and the immunocompromised. We sequenced the genomes of P. aeruginosa isolates RNS_PA1, RNS_PA46 and RNS_PAE05, which displayed resistance to almost all frontline antibiotics, including gentamicin, piperacillin, timentin, meropenem, ceftazidime and colistin. We provide evidence that the isolates are representatives of P. aeruginosa sequence type (ST) 235 and carry Tn 6162 and Tn 6163 in genomic islands 1 (GI1) and 2 (GI2), respectively. GI1 disrupts the endA gene at precisely the same chromosomal location as in P. aeruginosa strain VR-143/97, of unknown ST, creating an identical CA direct repeat. The class 1 integron associated with Tn 6163 in GI2 carries a bla GES-5 – aacA4 – gcuE15 – aphA15 cassette array conferring resistance to carbapenems and aminoglycosides. GI2 is flanked by a 12 nt direct repeat motif, abuts a tRNA-gly gene, and encodes proteins with putative roles in integration, conjugative transfer as well as integrative conjugative element-specific proteins. This suggests that GI2 may have evolved from a novel integrative conjugative element. Our data provide further support to the hypothesis that genomic islands play an important role in de novo evolution of multiple antibiotic resistance phenotypes in P. aeruginosa .
APA, Harvard, Vancouver, ISO, and other styles
15

Calcutt, Michael J., Michelle S. Lewis, and Kim S. Wise. "Molecular Genetic Analysis of ICEF, an Integrative Conjugal Element That Is Present as a Repetitive Sequence in the Chromosome of Mycoplasma fermentans PG18." Journal of Bacteriology 184, no. 24 (December 15, 2002): 6929–41. http://dx.doi.org/10.1128/jb.184.24.6929-6941.2002.

Full text
Abstract:
ABSTRACT Mycoplasma genomes contain compact gene sets that approach the minimal complement necessary for life and reflect multiple evolutionary instances of genomic reduction. Lateral gene transfer may play a critical role in shaping the mobile gene pool in these organisms, yet complex mobile elements have not been reported within this genus. We describe here a large (∼23-kb) genetic element with unique features that is present in four copies in the Mycoplasma fermentans PG18 chromosome, accounting for approximately 8% of the genome. These novel elements, designated ICEF (integrative conjugal elements of M. fermentans), resemble conjugative, self-transmissible integrating elements (constins) in that circular, nonreplicative extrachromosomal forms occur in which the left and right termini of the integrated element are juxtaposed and separated by a coupling sequence derived from direct repeats flanking chromosomal copies of ICEF as a result of target site duplication. ICEF contain multiple similarly oriented open reading frames (ORFs), of which some have homology to products of known conjugation genes but others have no known counterparts. Surprisingly, unlike other constins, ICEF lack homologs of known integrases, transposases, or recombinases, suggesting that a novel enzyme may be employed for integration-excision. Skewed distribution and varied sites of chromosomal integration among M. fermentans isolates suggest a role for ICEF in promoting genomic and phenotypic variation in this species. Identification of homologs of terminal ICEF ORFs in two additional mycoplasma species indicates that ICEF is the prototype member of a family of ICE-related elements that may be widespread among pathogenic mycoplasmas infecting diverse vertebrate hosts.
APA, Harvard, Vancouver, ISO, and other styles
16

Leonetti, Cori T., Matt A. Hamada, Stephanie J. Laurer, Matthew P. Broulidakis, Kyle J. Swerdlow, Catherine A. Lee, Alan D. Grossman, and Melanie B. Berkmen. "Critical Components of the Conjugation Machinery of the Integrative and Conjugative Element ICEBs1of Bacillus subtilis." Journal of Bacteriology 197, no. 15 (May 26, 2015): 2558–67. http://dx.doi.org/10.1128/jb.00142-15.

Full text
Abstract:
ABSTRACTConjugation, or mating, plays a profound role in bacterial evolution by spreading genes that allow bacteria to adapt to and colonize new niches. ICEBs1, an integrative and conjugative element ofBacillus subtilis, can transfer itself and mobilize resident plasmids. DNA transfer is mediated by a type IV secretion system (T4SS). Characterized components of the ICEBs1T4SS include the conserved VirB4-like ATPase ConE, the bifunctional cell wall hydrolase CwlT, and the presumed VirD4-like coupling protein ConQ. A fusion of ConE to green fluorescent protein (GFP) localizes to the membrane preferentially at the cell poles. One or more ICEBs1proteins are required for ConE's localization at the membrane, as ConE lacks predicted transmembrane segments and ConE-GFP is found dispersed throughout the cytoplasm in cells lacking ICEBs1. Here, we analyzed five ICEBs1genes to determine if they are required for DNA transfer and/or ConE-GFP localization. We found thatconB,conC,conD, andconG, but notyddF, are required for both ICEBs1transfer and plasmid mobilization. All four required genes encode predicted integral membrane proteins.conBand, to some extent,conDwere required for localization of ConE-GFP to the membrane. Using an adenylate cyclase-based bacterial two-hybrid system, we found that ConE interacts with ConB. We propose a model in which the ICEBs1conjugation machinery is composed of ConB, ConC, ConD, ConE, ConG, CwlT, ConQ, and possibly other ICEBs1proteins, and that ConB interacts with ConE, helping to recruit and/or maintain ConE at the membrane.IMPORTANCEConjugation is a major form of horizontal gene transfer and has played a profound role in bacterial evolution by moving genes, including those involved in antibiotic resistance, metabolism, symbiosis, and infectious disease. During conjugation, DNA is transferred from cell to cell through the conjugation machinery, a type of secretion system. Relatively little is known about the conjugation machinery of Gram-positive bacteria. Here, we analyzed five genes of the integrative and conjugative element ICEBs1ofBacillus subtilis. Our research identifies four new components of the ICEBs1conjugation machinery (ConB, ConC, ConD, and ConG) and shows an interaction between ConB and ConE that is required for ConE to associate with the cell membrane.
APA, Harvard, Vancouver, ISO, and other styles
17

Johnson, Christopher M., and Alan D. Grossman. "The Composition of the Cell Envelope Affects Conjugation in Bacillus subtilis." Journal of Bacteriology 198, no. 8 (February 1, 2016): 1241–49. http://dx.doi.org/10.1128/jb.01044-15.

Full text
Abstract:
ABSTRACTConjugation in bacteria is the contact-dependent transfer of DNA from one cell to another via donor-encoded conjugation machinery. It is a major type of horizontal gene transfer between bacteria. Conjugation of the integrative and conjugative element ICEBs1intoBacillus subtilisis affected by the composition of phospholipids in the cell membranes of the donor and recipient. We found that reduction (or elimination) of lysyl-phosphatidylglycerol caused by loss ofmprFcaused a decrease in conjugation efficiency. Conversely, alterations that caused an increase in lysyl-phosphatidylglycerol, including loss ofugtPor overproduction ofmprF, caused an increase in conjugation efficiency. In addition, we found that mutations that alter production of other phospholipids, e.g., loss ofclsAandyfnI, also affected conjugation, apparently without substantively altering levels of lysyl-phosphatidylglycerol, indicating that there are multiple pathways by which changes to the cell envelope affect conjugation. We found that the contribution ofmprFto conjugation was affected by the chemical environment. Wild-type cells were generally more responsive to addition of anions that enhanced conjugation, whereasmprFmutant cells were more sensitive to combinations of anions that inhibited conjugation at pH 7. Our results indicate thatmprFand lysyl-phosphatidylglycerol allow cells to maintain relatively consistent conjugation efficiencies under a variety of ionic conditions.IMPORTANCEHorizontal gene transfer is a driving force in microbial evolution, enabling cells that receive DNA to acquire new genes and phenotypes. Conjugation, the contact-dependent transfer of DNA from a donor to a recipient by a donor-encoded secretion machine, is a prevalent type of horizontal gene transfer. Although critically important, it is not well understood how the recipient influences the success of conjugation. We found that the composition of phospholipids in the membranes of donors and recipients influences the success of transfer of the integrative and conjugative element ICEBs1inBacillus subtilis. Specifically, the presence of lysyl-phosphatidylglycerol enables relatively constant conjugation efficiencies in a range of diverse chemical environments.
APA, Harvard, Vancouver, ISO, and other styles
18

Giovanetti, Eleonora, Andrea Brenciani, Erika Tiberi, Alessandro Bacciaglia, and Pietro Emanuele Varaldo. "ICESp2905, theerm(TR)-tet(O) Element of Streptococcus pyogenes, Is Formed by Two Independent Integrative and Conjugative Elements." Antimicrobial Agents and Chemotherapy 56, no. 1 (October 10, 2011): 591–94. http://dx.doi.org/10.1128/aac.05352-11.

Full text
Abstract:
ABSTRACTIn ICESp2905, a widespreaderm(TR)- andtet(O)-carrying genetic element ofStreptococcus pyogenes, the two resistance determinants are contained in separate fragments inserted into a scaffold of clostridial origin. ICESp2905(∼65.6 kb) was transferable not only in its regular form but also in a defective form lacking theerm(TR) fragment (ICESp2906, ∼53.0 kb). Theerm(TR) fragment was also an independent integrative and conjugative element (ICE) (ICESp2907, ∼12.6 kb). ICESp2905thus results from one ICE (ICESp2907) being integrated into another (ICESp2906).
APA, Harvard, Vancouver, ISO, and other styles
19

Carraro, Nicolas, Virginie Libante, Catherine Morel, Florence Charron-Bourgoin, Pierre Leblond, and Gérard Guédon. "Plasmid-like replication of a minimal streptococcal integrative and conjugative element." Microbiology 162, no. 4 (April 1, 2016): 622–32. http://dx.doi.org/10.1099/mic.0.000219.

Full text
APA, Harvard, Vancouver, ISO, and other styles
20

Roche, David, Maud Fléchard, Nathalie Lallier, Maryline Répérant, Annie Brée, Géraldine Pascal, Catherine Schouler, and Pierre Germon. "ICEEc2, a New Integrative and Conjugative Element Belonging to the pKLC102/PAGI-2 Family, Identified in Escherichia coli Strain BEN374." Journal of Bacteriology 192, no. 19 (July 30, 2010): 5026–36. http://dx.doi.org/10.1128/jb.00609-10.

Full text
Abstract:
ABSTRACT The diversity of the Escherichia coli species is in part due to the large number of mobile genetic elements that are exchanged between strains. We report here the identification of a new integrative and conjugative element (ICE) of the pKLC102/PAGI-2 family located downstream of the tRNA gene pheU in the E. coli strain BEN374. Indeed, this new region, which we called ICEEc2, can be transferred by conjugation from strain BEN374 to the E. coli strain C600. We were also able to transfer this region into a Salmonella enterica serovar Typhimurium strain and into a Yersinia pseudotuberculosis strain. This transfer was then followed by the integration of ICEEc2 into the host chromosome downstream of a phe tRNA gene. Our data indicated that this transfer involved a set of three genes encoding DNA mobility enzymes and a type IV pilus encoded by genes present on ICEEc2. Given the wide distribution of members of this family, these mobile genetic elements are likely to play an important role in the diversification of bacteria.
APA, Harvard, Vancouver, ISO, and other styles
21

Han, Xiaoyan, Xiang-Dang Du, Luke Southey, Dieter M. Bulach, Torsten Seemann, Xu-Xia Yan, Trudi L. Bannam, and Julian I. Rood. "Functional Analysis of a Bacitracin Resistance Determinant Located on ICECp1, a Novel Tn916-Like Element from a Conjugative Plasmid in Clostridium perfringens." Antimicrobial Agents and Chemotherapy 59, no. 11 (August 17, 2015): 6855–65. http://dx.doi.org/10.1128/aac.01643-15.

Full text
Abstract:
ABSTRACTBacitracins are mixtures of structurally related cyclic polypeptides with antibiotic properties. They act by interfering with the biosynthesis of the bacterial cell wall. In this study, we analyzed an avian necrotic enteritis strain ofClostridium perfringensthat was resistant to bacitracin and produced NetB toxin. We identified a bacitracin resistance locus that resembled a bacitracin resistance determinant fromEnterococcus faecalis. It contained the structural genesbcrABDand a putative regulatory gene,bcrR. Mutagenesis studies provided evidence that bothbcrAandbcrBare essential for bacitracin resistance, and that evidence was supported by the results of experiments in which the introduction of both thebcrAandbcrBgenes into a bacitracin-susceptibleC. perfringensstrain was required to confer bacitracin resistance. The wild-type strain was shown to contain at least three large, putatively conjugative plasmids, and thebcrRABDlocus was localized to an 89.7-kb plasmid, pJIR4150. This plasmid was experimentally shown to be conjugative and was sequenced. The sequence revealed that it also carries atpeLtoxin gene and is related to the pCW3 family of conjugative antibiotic resistance and toxin plasmids fromC. perfringens. Thebcrgenes were located on a genetic element, ICECp1, which is related to the Tn916family of integrative conjugative elements (ICEs). ICECp1appears to be the first Tn916-like element shown to confer bacitracin resistance. In summary, we identified in a toxin-producingC. perfringensstrain a novel mobile bacitracin resistance element which was experimentally shown to be essential for bacitracin resistance and is carried by a putative ICE located on a conjugative plasmid.
APA, Harvard, Vancouver, ISO, and other styles
22

McKeithen-Mead, Saria A., and Alan D. Grossman. "Timing of integration into the chromosome is critical for the fitness of an integrative and conjugative element and its bacterial host." PLOS Genetics 19, no. 2 (February 13, 2023): e1010524. http://dx.doi.org/10.1371/journal.pgen.1010524.

Full text
Abstract:
Integrative and conjugative elements (ICEs) are major contributors to genome plasticity in bacteria. ICEs reside integrated in the chromosome of a host bacterium and are passively propagated during chromosome replication and cell division. When activated, ICEs excise from the chromosome and may be transferred through the ICE-encoded conjugation machinery into a recipient cell. Integration into the chromosome of the new host generates a stable transconjugant. Although integration into the chromosome of a new host is critical for the stable acquisition of ICEs, few studies have directly investigated the molecular events that occur in recipient cells during generation of a stable transconjugant. We found that integration of ICEBs1, an ICE of Bacillus subtilis, occurred several generations after initial transfer to a new host. Premature integration in new hosts led to cell death and hence decreased fitness of the ICE and transconjugants. Host lethality due to premature integration was caused by rolling circle replication that initiated in the integrated ICEBs1 and extended into the host chromosome, resulting in catastrophic genome instability. Our results demonstrate that the timing of integration of an ICE is linked to cessation of autonomous replication of the ICE, and that perturbing this linkage leads to a decrease in ICE and host fitness due to a loss of viability of transconjugants. Linking integration to cessation of autonomous replication appears to be a conserved regulatory scheme for mobile genetic elements that both replicate and integrate into the chromosome of their host.
APA, Harvard, Vancouver, ISO, and other styles
23

Marenda, Marc, Valérie Barbe, Géraldine Gourgues, Sophie Mangenot, Evelyne Sagne, and Christine Citti. "A New Integrative Conjugative Element Occurs in Mycoplasma agalactiae as Chromosomal and Free Circular Forms." Journal of Bacteriology 188, no. 11 (June 1, 2006): 4137–41. http://dx.doi.org/10.1128/jb.00114-06.

Full text
Abstract:
ABSTRACT An integrative conjugative element, ICEA, was characterized in Mycoplasma agalactiae strain 5632, in which it occurs as multiple chromosomal copies and as a free circular form. The distribution of ICEA sequences in M. agalactiae strains and their occurrence in Mycoplasma bovis suggest the spreading of the element within or between species.
APA, Harvard, Vancouver, ISO, and other styles
24

Mingoia, Marina, Eleonora Morici, Gianluca Morroni, Eleonora Giovanetti, Maria Del Grosso, Annalisa Pantosti, and Pietro E. Varaldo. "Tn5253Family Integrative and Conjugative Elements Carryingmef(I) andcatQDeterminants in Streptococcus pneumoniae and Streptococcus pyogenes." Antimicrobial Agents and Chemotherapy 58, no. 10 (July 28, 2014): 5886–93. http://dx.doi.org/10.1128/aac.03638-14.

Full text
Abstract:
ABSTRACTThe linkage between the macrolide efflux genemef(I) and the chloramphenicol inactivation genecatQwas first described inStreptococcus pneumoniae(strain Spn529), where the two genes are located in a module designated IQ element. Subsequently, two different defective IQ elements were detected inStreptococcus pyogenes(strains Spy029 and Spy005). The genetic elements carrying the three IQ elements were characterized, and all were found to be Tn5253family integrative and conjugative elements (ICEs). The ICE fromS. pneumoniae(ICESpn529IQ) was sequenced, whereas the ICEs fromS. pyogenes(ICESpy029IQ and ICESpy005IQ, the first Tn5253-like ICEs reported in this species) were characterized by PCR mapping, partial sequencing, and restriction analysis. ICESpn529IQ and ICESpy029IQ were found to share theintSp23FST81integrase gene and an identical Tn916fragment, whereas ICESpy005IQ hasint5252and lacks Tn916. All three ICEs were found to lack the linearized pC194 plasmid that is usually associated with Tn5253-like ICEs, and all displayed a single copy of a toxin-antitoxin operon that is typically contained in the direct repeats flanking the excisable pC194 region when this region is present. Two different insertion sites of the IQ elements were detected, one in ICESpn529IQ and ICESpy029IQ, and another in ICESpy005IQ. The chromosomal integration of the three ICEs was site specific, depending on the integrase (intSp23FST81orint5252). Only ICESpy005IQ was excised in circular form and transferred by conjugation. By transformation,mef(I) andcatQwere cotransferred at a high frequency fromS. pyogenesSpy005 and at very low frequencies fromS. pneumoniaeSpn529 andS. pyogenesSpy029.
APA, Harvard, Vancouver, ISO, and other styles
25

Sezonov, Guennadi, Christophe Possoz, Annick Friedmann, Jean-Luc Pernodet, and Michel Guérineau. "KorSA from the Streptomyces Integrative Element pSAM2 Is a Central Transcriptional Repressor: Target Genes and Binding Sites." Journal of Bacteriology 182, no. 5 (March 1, 2000): 1243–50. http://dx.doi.org/10.1128/jb.182.5.1243-1250.2000.

Full text
Abstract:
ABSTRACT pSAM2, a 10.9-kb mobile integrative genetic element fromStreptomyces ambofaciens, possesses, as do a majority ofStreptomyces conjugative plasmids, a kil-korsystem associated with its transfer. The kor function of pSAM2 was attributed to the korSA gene, but its direct role remained unclear. The present study was focused on the determination of the KorSA targets. It was shown that KorSA acts as a transcriptional repressor by binding to a conserved 17-nucleotide sequence found upstream of only two genes: its own gene, korSA, andpra, a gene positively controlling pSAM2 replication, integration, and excision. A unique feature of KorSA, compared to Kor proteins from other Streptomyces conjugative plasmids, is that it does not directly regulate pSAM2 transfer. KorSA does not bind to the pSAM2 genes coding for transfer and intramycelial spreading. Through the repression of pra, KorSA is able to negatively regulate pSAM2 functions activated by Pra and, consequently, to maintain pSAM2 integrated in the chromosome.
APA, Harvard, Vancouver, ISO, and other styles
26

Wozniak, Rachel A. F., and Matthew K. Waldor. "A Toxin–Antitoxin System Promotes the Maintenance of an Integrative Conjugative Element." PLoS Genetics 5, no. 3 (March 27, 2009): e1000439. http://dx.doi.org/10.1371/journal.pgen.1000439.

Full text
APA, Harvard, Vancouver, ISO, and other styles
27

Michael, G. B., K. Kadlec, M. T. Sweeney, E. Brzuszkiewicz, H. Liesegang, R. Daniel, R. W. Murray, J. L. Watts, and S. Schwarz. "ICEPmu1, an integrative conjugative element (ICE) of Pasteurella multocida: structure and transfer." Journal of Antimicrobial Chemotherapy 67, no. 1 (October 14, 2011): 91–100. http://dx.doi.org/10.1093/jac/dkr411.

Full text
APA, Harvard, Vancouver, ISO, and other styles
28

Auchtung, Jennifer M., Naira Aleksanyan, Artemisa Bulku, and Melanie B. Berkmen. "Biology of ICE Bs1 , an integrative and conjugative element in Bacillus subtilis." Plasmid 86 (July 2016): 14–25. http://dx.doi.org/10.1016/j.plasmid.2016.07.001.

Full text
APA, Harvard, Vancouver, ISO, and other styles
29

Aberkane, Salim, Fabrice Compain, Dominique Decré, Chloé Dupont, Chrislène Laurens, Marion Vittecoq, Alix Pantel, et al. "High Prevalence of SXT/R391-Related Integrative and Conjugative Elements CarryingblaCMY-2in Proteus mirabilis Isolates from Gulls in the South of France." Antimicrobial Agents and Chemotherapy 60, no. 2 (December 7, 2015): 1148–52. http://dx.doi.org/10.1128/aac.01654-15.

Full text
Abstract:
ABSTRACTThe genetic structures involved in the dissemination ofblaCMY-2carried byProteus mirabilisisolates recovered from different gull species in the South of France were characterized and compared to clinical isolates.blaCMY-2was identified inP. mirabilisisolates from 27/93 yellow-legged gulls and from 37/65 slender-billed gulls. It was carried by a conjugative SXT/R391-like integrative and conjugative element (ICE) in all avian strains and in 3/7 human strains. Two clinical isolates had the same genetic background as six avian isolates.
APA, Harvard, Vancouver, ISO, and other styles
30

Johnson, Christopher M., M. Michael Harden, and Alan D. Grossman. "Interactions between mobile genetic elements: An anti-phage gene in an integrative and conjugative element protects host cells from predation by a temperate bacteriophage." PLOS Genetics 18, no. 2 (February 14, 2022): e1010065. http://dx.doi.org/10.1371/journal.pgen.1010065.

Full text
Abstract:
Most bacterial genomes contain horizontally acquired and transmissible mobile genetic elements, including temperate bacteriophages and integrative and conjugative elements. Little is known about how these elements interact and co-evolved as parts of their host genomes. In many cases, it is not known what advantages, if any, these elements provide to their bacterial hosts. Most strains of Bacillus subtilis contain the temperate phage SPß and the integrative and conjugative element ICEBs1. Here we show that the presence of ICEBs1 in cells protects populations of B. subtilis from predation by SPß, likely providing selective pressure for the maintenance of ICEBs1 in B. subtilis. A single gene in ICEBs1 (yddK, now called spbK for SPß killing) was both necessary and sufficient for this protection. spbK inhibited production of SPß, during both activation of a lysogen and following de novo infection. We found that expression spbK, together with the SPß gene yonE constitutes an abortive infection system that leads to cell death. spbK encodes a TIR (Toll-interleukin-1 receptor)-domain protein with similarity to some plant antiviral proteins and animal innate immune signaling proteins. We postulate that many uncharacterized cargo genes in ICEs may confer selective advantage to cells by protecting against other mobile elements.
APA, Harvard, Vancouver, ISO, and other styles
31

Puymege, A., S. Bertin, S. Chuzeville, G. Guedon, and S. Payot. "Conjugative Transfer and cis-Mobilization of a Genomic Island by an Integrative and Conjugative Element of Streptococcus agalactiae." Journal of Bacteriology 195, no. 6 (December 28, 2012): 1142–51. http://dx.doi.org/10.1128/jb.02199-12.

Full text
APA, Harvard, Vancouver, ISO, and other styles
32

Bean, Emily L., Lisa K. McLellan, and Alan D. Grossman. "Activation of the integrative and conjugative element Tn916 causes growth arrest and death of host bacteria." PLOS Genetics 18, no. 10 (October 24, 2022): e1010467. http://dx.doi.org/10.1371/journal.pgen.1010467.

Full text
Abstract:
Integrative and conjugative elements (ICEs) serve as major drivers of bacterial evolution. These elements often confer some benefit to host cells, including antibiotic resistance, metabolic capabilities, or pathogenic determinants. ICEs can also have negative effects on host cells. Here, we investigated the effects of the ICE (conjugative transposon) Tn916 on host cells. Because Tn916 is active in a relatively small subpopulation of host cells, we developed a fluorescent reporter system for monitoring activation of Tn916 in single cells. Using this reporter, we found that cell division was arrested in cells of Bacillus subtilis and Enterococcus faecalis (a natural host for Tn916) that contained an activated (excised) Tn916. Furthermore, most of the cells with the activated Tn916 subsequently died. We also observed these phenotypes on the population level in B. subtilis utilizing a modified version of Tn916 that can be activated in the majority of cells. We identified two genes (orf17 and orf16) in Tn916 that were sufficient to cause growth defects in B. subtilis and identified a single gene, yqaR, that is in a defective phage (skin) in the B. subtilis chromosome that was required for this phenotype. These three genes were only partially responsible for the growth defect caused by Tn916, indicating that Tn916 possesses multiple mechanisms to affect growth and viability of host cells. These results highlight the complex relationships that conjugative elements have with their host cells and the interplay between mobile genetic elements.
APA, Harvard, Vancouver, ISO, and other styles
33

te Poele, Evelien M., Harm Kloosterman, Gerda I. Hessels, Henk Bolhuis, and Lubbert Dijkhuizen. "RepAM of the Amycolatopsis methanolica integrative element pMEA300 belongs to a novel class of replication initiator proteins." Microbiology 152, no. 10 (October 1, 2006): 2943–50. http://dx.doi.org/10.1099/mic.0.28746-0.

Full text
Abstract:
Accessory genetic elements, such as plasmids and integrative elements, are widespread amongst actinomycetes, but little is known about their functions and mode of replication. The conjugative element pMEA300 from Amycolatopsis methanolica is present mostly in an integrated state at a single specific site in the chromosome, but it can also replicate autonomously. Complete nucleotide sequencing, in combination with deletion studies, has revealed that orfB of pMEA300 is essential for autonomous replication in its host. In this study, it was shown that purified OrfB protein binds specifically to the 3′ end of its own coding sequence. Within this short sequence, a putative hairpin structure is located, which contains several direct and inverted repeats, and a nucleotide stretch that resembles the nicking site of the pC194 family of rolling circle replicating plasmids. Additional binding studies revealed that OrfB binds to an 8 bp inverted repeat that occurs three times within the hairpin structure. The data presented show that OrfB is the replication initiator (Rep) protein of pMEA300, and is therefore termed RepAM. Surprisingly, RepAM lacks significant sequence similarity with known prokaryotic Rep proteins, but it is highly similar to a number of yet uncharacterized ORFs that are located on integrative and conjugative elements of other actinomycetes. It is concluded that RepAM and its homologues are members of a novel class of Rep proteins.
APA, Harvard, Vancouver, ISO, and other styles
34

Sugimoto, Yuta, Fumito Maruyama, and Satoru Suzuki. "Draft Genome Sequence of a Shewanella halifaxensis Strain Isolated from the Intestine of Marine Red Seabream (Pagrus major), Which Includes an Integrative Conjugative Element with Macrolide Resistance Genes." Genome Announcements 6, no. 16 (April 19, 2018): e00297-18. http://dx.doi.org/10.1128/genomea.00297-18.

Full text
Abstract:
ABSTRACT Shewanella halifaxensis strain 6JANF4-E-4 was isolated from the intestine of a red seabream (Pagrus major). Here, we report the draft genome sequence of this bacterium, which includes an integrative conjugative element of the SXT/R391 family, where the macrolide resistance determinants mef(C) and mph(G) exist.
APA, Harvard, Vancouver, ISO, and other styles
35

Thomas, Jacob, Catherine A. Lee, and Alan D. Grossman. "A Conserved Helicase Processivity Factor Is Needed for Conjugation and Replication of an Integrative and Conjugative Element." PLoS Genetics 9, no. 1 (January 10, 2013): e1003198. http://dx.doi.org/10.1371/journal.pgen.1003198.

Full text
APA, Harvard, Vancouver, ISO, and other styles
36

Blesa, Alba, Ignacio Baquedano, Sandra González-de la Fuente, Mario Mencía, and José Berenguer. "Integrative and Conjugative Element ICETh1 Functions as a Pangenomic DNA Capture Module in Thermus thermophilus." Microorganisms 8, no. 12 (December 21, 2020): 2051. http://dx.doi.org/10.3390/microorganisms8122051.

Full text
Abstract:
Transjugation is an unconventional conjugation mechanism in Thermus thermophilus (Tth) that involves the active participation of both mating partners, encompassing a DNA secretion system (DSS) in the donor and an active natural competence apparatus (NCA) in the recipient cells. DSS is encoded within an integrative and conjugative element (ICETh1) in the strain Tth HB27, whereas the NCA is constitutively expressed in both mates. Previous experiments suggested the presence of multiple origins of transfer along the genome, which could generate genomic mosaicity among the progeny. Here, we designed transjugation experiments between two closely related strains of Tth with highly syntenic genomes, containing enough single nucleotide polymorphisms to allow precise parenthood analysis. Individual clones from the progeny were sequenced, revealing their origin as derivatives of our ICETh1-containing intended “donor” strain (HB27), which had acquired separate fragments from the genome of the ICETh1-free HB8 cells, which are our intended recipient. Due to the bidirectional nature of transjugation, only assays employing competence-defective HB27 derivatives as donors allowed the recovery of HB8-derived progeny. These results show a preference for a retrotransfer mechanism in transjugation in ICETh1-bearing strains, supporting an inter-strain gene-capture function for ICETh1. This function could benefit the donor-capable host by facilitating the acquisition of adaptive traits from external sources, ultimately increasing the open pangenome of Thermus, maximizing the potential repertoire of physiological and phenotypical traits related to adaptation and speciation.
APA, Harvard, Vancouver, ISO, and other styles
37

Podshivalova, M. V., Yu A. Kuzyutina, I. B. Zakharova, Ya A. Lopasteyskaya, and D. V. Viktorov. "Characteristics of antibiotic resistant strains of Vibrio cholerae carrying SXT type integrative conjugative elements." Epidemiology and Infectious Diseases 19, no. 3 (June 15, 2014): 34–39. http://dx.doi.org/10.17816/eid40820.

Full text
Abstract:
In the paper there is presented a characteristics of antibiotic-resistant strains of Vibrio cholerae, isolated in the Volgograd region during the period of 1980-2000. There were studied cultural and morphological properties of the isolates, their biochemical activity, resistance to antibiotics of different classes, there was performed the detection of virulence genes and sequences of transmissible SXT-element. There was demonstrated the presence of different types of SXT in the content of the genome of the examined strains - SXT MO10 element with cluster of the antibiotic resistance gene sulII-strB-dfr18, SXT ET element carrying the sequences sulII dfrA1, and not having a resistance gene to aminoglycosides strB, and SXT S element with deleted cluster of antibiotic resistance genes.
APA, Harvard, Vancouver, ISO, and other styles
38

Bean, Emily L., Calvin Herman, Mary E. Anderson, and Alan D. Grossman. "Biology and engineering of integrative and conjugative elements: Construction and analyses of hybrid ICEs reveal element functions that affect species-specific efficiencies." PLOS Genetics 18, no. 5 (May 18, 2022): e1009998. http://dx.doi.org/10.1371/journal.pgen.1009998.

Full text
Abstract:
Integrative and conjugative elements (ICEs) are mobile genetic elements that reside in a bacterial host chromosome and are prominent drivers of bacterial evolution. They are also powerful tools for genetic analyses and engineering. Transfer of an ICE to a new host involves many steps, including excision from the chromosome, DNA processing and replication, transfer across the envelope of the donor and recipient, processing of the DNA, and eventual integration into the chromosome of the new host (now a stable transconjugant). Interactions between an ICE and its host throughout the life cycle likely influence the efficiencies of acquisition by new hosts. Here, we investigated how different functional modules of two ICEs, Tn916 and ICEBs1, affect the transfer efficiencies into different host bacteria. We constructed hybrid elements that utilize the high-efficiency regulatory and excision modules of ICEBs1 and the conjugation genes of Tn916. These elements produced more transconjugants than Tn916, likely due to an increase in the number of cells expressing element genes and a corresponding increase in excision. We also found that several Tn916 and ICEBs1 components can substitute for one other. Using B. subtilis donors and three Enterococcus species as recipients, we found that different hybrid elements were more readily acquired by some species than others, demonstrating species-specific interactions in steps of the ICE life cycle. This work demonstrates that hybrid elements utilizing the efficient regulatory functions of ICEBs1 can be built to enable efficient transfer into and engineering of a variety of other species.
APA, Harvard, Vancouver, ISO, and other styles
39

Ramsay, Joshua P., Tahlia R. Bastholm, Callum J. Verdonk, Dinah D. Tambalo, John T. Sullivan, Liam K. Harold, Beatrice A. Panganiban, et al. "An epigenetic switch activates bacterial quorum sensing and horizontal transfer of an integrative and conjugative element." Nucleic Acids Research 50, no. 2 (December 14, 2021): 975–88. http://dx.doi.org/10.1093/nar/gkab1217.

Full text
Abstract:
Abstract Horizontal transfer of the integrative and conjugative element ICEMlSymR7A converts non-symbiotic Mesorhizobium spp. into nitrogen-fixing legume symbionts. Here, we discover subpopulations of Mesorhizobium japonicum R7A become epigenetically primed for quorum-sensing (QS) and QS-activated horizontal transfer. Isolated populations in this state termed R7A* maintained these phenotypes in laboratory culture but did not transfer the R7A* state to recipients of ICEMlSymR7A following conjugation. We previously demonstrated ICEMlSymR7A transfer and QS are repressed by the antiactivator QseM in R7A populations and that the adjacently-coded DNA-binding protein QseC represses qseM transcription. Here RNA-sequencing revealed qseM expression was repressed in R7A* cells and that RNA antisense to qseC was abundant in R7A but not R7A*. Deletion of the antisense-qseC promoter converted cells into an R7A*-like state. An adjacently coded QseC2 protein bound two operator sites and repressed antisense-qseC transcription. Plasmid overexpression of QseC2 stimulated the R7A* state, which persisted following curing of this plasmid. The epigenetic maintenance of the R7A* state required ICEMlSymR7A-encoded copies of both qseC and qseC2. Therefore, QseC and QseC2, together with their DNA-binding sites and overlapping promoters, form a stable epigenetic switch that establishes binary control over qseM transcription and primes a subpopulation of R7A cells for QS and horizontal transfer.
APA, Harvard, Vancouver, ISO, and other styles
40

Grohmann, Elisabeth. "Conjugative Transfer of the Integrative and Conjugative Element ICEBs1 from Bacillus subtilis Likely Initiates at the Donor Cell Pole." Journal of Bacteriology 192, no. 1 (October 23, 2009): 23–25. http://dx.doi.org/10.1128/jb.01305-09.

Full text
APA, Harvard, Vancouver, ISO, and other styles
41

Shang, Yanhong, Dexi Li, Wenbo Hao, Stefan Schwarz, Xinxin Shan, Bianzhi Liu, Su-Mei Zhang, Xin-Sheng Li, and Xiang-Dang Du. "A prophage and two ICESa2603-family integrative and conjugative elements (ICEs) carrying optrA in Streptococcus suis." Journal of Antimicrobial Chemotherapy 74, no. 10 (July 17, 2019): 2876–79. http://dx.doi.org/10.1093/jac/dkz309.

Full text
Abstract:
Abstract Objectives To investigate the presence and transfer of the oxazolidinone/phenicol resistance gene optrA and identify the genetic elements involved in the horizontal transfer of the optrA gene in Streptococcus suis. Methods A total of 237 S. suis isolates were screened for the presence of the optrA gene by PCR. Whole-genome DNA of three optrA-positive strains was completely sequenced using the Illumina MiSeq and Pacbio RSII platforms. MICs were determined by broth microdilution. Transferability of the optrA gene in S. suis was investigated by conjugation. The presence of circular intermediates was examined by inverse PCR. Results The optrA gene was present in 11.8% (28/237) of the S. suis strains. In three strains, the optrA gene was flanked by two copies of IS1216 elements in the same orientation, located either on a prophage or on ICESa2603-family integrative and conjugative elements (ICEs), including one tandem ICE. In one isolate, the optrA-carrying ICE transferred with a frequency of 2.1 × 10−8. After the transfer, the transconjugant displayed elevated MICs of the respective antimicrobial agents. Inverse PCRs revealed that circular intermediates of different sizes were formed in the three optrA-carrying strains, containing one copy of the IS1216E element and the optrA gene alone or in combination with other resistance genes. Conclusions A prophage and two ICESa2603-family ICEs (including one tandem ICE) associated with the optrA gene were identified in S. suis. The association of the optrA gene with the IS1216E elements and its location on either a prophage or ICEs will aid its horizontal transfer.
APA, Harvard, Vancouver, ISO, and other styles
42

Huguet-Tapia, Jose C., Dawn R. D. Bignell, and Rosemary Loria. "Characterization of the Integration and Modular Excision of the Integrative Conjugative Element PAISt in Streptomyces turgidiscabies Car8." PLoS ONE 9, no. 6 (June 13, 2014): e99345. http://dx.doi.org/10.1371/journal.pone.0099345.

Full text
APA, Harvard, Vancouver, ISO, and other styles
43

Durand, Romain, Florence Deschênes, and Vincent Burrus. "Genomic islands targeting dusA in Vibrio species are distantly related to Salmonella Genomic Island 1 and mobilizable by IncC conjugative plasmids." PLOS Genetics 17, no. 8 (August 20, 2021): e1009669. http://dx.doi.org/10.1371/journal.pgen.1009669.

Full text
Abstract:
Salmonella Genomic Island 1 (SGI1) and its variants are significant contributors to the spread of antibiotic resistance among Gammaproteobacteria. All known SGI1 variants integrate at the 3’ end of trmE, a gene coding for a tRNA modification enzyme. SGI1 variants are mobilized specifically by conjugative plasmids of the incompatibility groups A and C (IncA and IncC). Using a comparative genomics approach based on genes conserved among members of the SGI1 group, we identified diverse integrative elements distantly related to SGI1 in several species of Vibrio, Aeromonas, Salmonella, Pokkaliibacter, and Escherichia. Unlike SGI1, these elements target two alternative chromosomal loci, the 5’ end of dusA and the 3’ end of yicC. Although they share many features with SGI1, they lack antibiotic resistance genes and carry alternative integration/excision modules. Functional characterization of IMEVchUSA3, a dusA-specific integrative element, revealed promoters that respond to AcaCD, the master activator of IncC plasmid transfer genes. Quantitative PCR and mating assays confirmed that IMEVchUSA3 excises from the chromosome and is mobilized by an IncC helper plasmid from Vibrio cholerae to Escherichia coli. IMEVchUSA3 encodes the AcaC homolog SgaC that associates with AcaD to form a hybrid activator complex AcaD/SgaC essential for its excision and mobilization. We identified the dusA-specific recombination directionality factor RdfN required for the integrase-mediated excision of dusA-specific elements from the chromosome. Like xis in SGI1, rdfN is under the control of an AcaCD-responsive promoter. Although the integration of IMEVchUSA3 disrupts dusA, it provides a new promoter sequence and restores the reading frame of dusA for proper expression of the tRNA-dihydrouridine synthase A. Phylogenetic analysis of the conserved proteins encoded by SGI1-like elements targeting dusA, yicC, and trmE gives a fresh perspective on the possible origin of SGI1 and its variants.
APA, Harvard, Vancouver, ISO, and other styles
44

Dahl, Kristin H., and Arnfinn Sundsfjord. "Transferable vanB2 Tn5382-Containing Elements in Fecal Streptococcal Strains from Veal Calves." Antimicrobial Agents and Chemotherapy 47, no. 8 (August 2003): 2579–83. http://dx.doi.org/10.1128/aac.47.8.2579-2583.2003.

Full text
Abstract:
ABSTRACT Three vancomycin-resistant veal calf fecal streptococci, identified as Streptococcus gallolyticus (n = 2) and Streptococcus lutetiensis, were shown to harbor vanB2 Tn5382-like elements earlier described in enterococci. One S. gallolyticus strain had a 1,495-bp IS256-related element inserted in vanSB . The vanB2 Tn5382 element present in the plasmid-free S. lutetiensis strain was transferable to Enterococcus faecium BM4105-RF, Enterococcus faecalis JH2-2, and its recombination-deficient derivative, UV202. The transfer frequencies were comparable between recipient strains (from 1 × 10−7 to 7 × 10−6). All transconjugants acquired a vanB-containing chromosomal insert of approximately 100 kb, apparently by site-specific integration. Secondary transconjugants were not observed in intraspecies retransfer experiments. These observations are consistent with a conjugative, selftransmissible, integrative element that might be involved in the interspecies spread of vanB2 resistance determinants. Two JH2-2-derived transconjugants had also gained additional copies of large vanB-containing chromosomal fragments, a process that involves unexplained mechanisms that seems to require functional host cell-dependent recombination mechanisms.
APA, Harvard, Vancouver, ISO, and other styles
45

Haenni, Marisa, Estelle Saras, Stéphane Bertin, Pierre Leblond, Jean-Yves Madec, and Sophie Payot. "Diversity and Mobility of Integrative and Conjugative Elements in Bovine Isolates of Streptococcusagalactiae, S. dysgalactiae subsp. dysgalactiae, and S. uberis." Applied and Environmental Microbiology 76, no. 24 (October 15, 2010): 7957–65. http://dx.doi.org/10.1128/aem.00805-10.

Full text
Abstract:
ABSTRACT Bovine isolates of S treptococcus agalactiae (n = 76), S treptococcus dysgalactiae subsp. dysgalactiae (n = 32), and S treptococcus uberis (n = 101) were analyzed for the presence of different integrative and conjugative elements (ICEs) and their association with macrolide, lincosamide, and tetracycline resistance. The diversity of the isolates included in this study was demonstrated by multilocus sequence typing for S. agalactiae and pulsed-field gel electrophoresis for S. dysgalactiae and S. uberis. Most of the erythromycin-resistant strains carry an ermB gene. Five strains of S. uberis that are resistant to lincomycin but susceptible to erythromycin carry the lin(B) gene, and one has both linB and lnuD genes. In contrast to S. uberis, most of the S. agalactiae and S. dysgalactiae tetracycline-resistant isolates carry a tet(M) gene. A tet(S) gene was also detected in the three species. A Tn916-related element was detected in 30 to 50% of the tetracycline-resistant strains in the three species. Tetracycline resistance was successfully transferred by conjugation to an S. agalactiae strain. Most of the isolates carry an ICE integrated in the rplL gene. In addition, half of the S. agalactiae isolates have an ICE integrated in a tRNA lysine (tRNALys) gene. Such an element is also present in 20% of the isolates of S. dysgalactiae and S. uberis. A circular form of these ICEs was detected in all of the isolates tested, indicating that these genetic elements are mobile. These ICEs could thus also be a vehicle for horizontal gene transfer between streptococci of animal and/or human origin.
APA, Harvard, Vancouver, ISO, and other styles
46

Levicán, Gloria J., Assaf Katz, Jorge H. Valdés, Raquel Quatrini, David S. Holmes, and O. Orellana. "A 300 kpb Genome Segment, Including a Complete Set of tRNA Genes, is Dispensable for Acidithiobacillus Ferrooxidans." Advanced Materials Research 71-73 (May 2009): 187–90. http://dx.doi.org/10.4028/www.scientific.net/amr.71-73.187.

Full text
Abstract:
The genome sequences from two strains of the acidophilic, autotrophic, chemolithotrophic proteobacterium A. ferrooxidans are available from genome databases. Bioinformatic sequence comparison revealed the existence in one strain of a putative integrative conjugative element (ICE), containing an entire set of clustered tRNA genes. ICE is missing in the other strain, suggesting that this element as well as the tRNA genes cluster is dispensable for the bacterium. Bioinformatic predictions suggest that the tRNA genes cluster might mainly contribute to the translation of ICE encoded genes.
APA, Harvard, Vancouver, ISO, and other styles
47

Zamarro, María Teresa, Zaira Martín-Moldes, and Eduardo Díaz. "The ICEXTDofAzoarcussp. CIB, an integrative and conjugative element with aerobic and anaerobic catabolic properties." Environmental Microbiology 18, no. 12 (August 30, 2016): 5018–31. http://dx.doi.org/10.1111/1462-2920.13465.

Full text
APA, Harvard, Vancouver, ISO, and other styles
48

Miyazaki, Ryo, Marco Minoia, Nicolas Pradervand, Sandra Sulser, Friedrich Reinhard, and Jan Roelof van der Meer. "Cellular Variability of RpoS Expression Underlies Subpopulation Activation of an Integrative and Conjugative Element." PLoS Genetics 8, no. 7 (July 12, 2012): e1002818. http://dx.doi.org/10.1371/journal.pgen.1002818.

Full text
APA, Harvard, Vancouver, ISO, and other styles
49

Reinhard, F., and J. R. van der Meer. "Life History Analysis of Integrative and Conjugative Element Activation in Growing Microcolonies of Pseudomonas." Journal of Bacteriology 196, no. 7 (January 24, 2014): 1425–34. http://dx.doi.org/10.1128/jb.01333-13.

Full text
APA, Harvard, Vancouver, ISO, and other styles
50

Gaillard, Muriel, Tatiana Vallaeys, Frank Jörg Vorhölter, Marco Minoia, Christoph Werlen, Vladimir Sentchilo, Alfred Pühler, and Jan Roelof van der Meer. "The clc Element of Pseudomonas sp. Strain B13, a Genomic Island with Various Catabolic Properties." Journal of Bacteriology 188, no. 5 (March 1, 2006): 1999–2013. http://dx.doi.org/10.1128/jb.188.5.1999-2013.2006.

Full text
Abstract:
ABSTRACT Pseudomonas sp. strain B13 is a bacterium known to degrade chloroaromatic compounds. The properties to use 3- and 4-chlorocatechol are determined by a self-transferable DNA element, the clc element, which normally resides at two locations in the cell's chromosome. Here we report the complete nucleotide sequence of the clc element, demonstrating the unique catabolic properties while showing its relatedness to genomic islands and integrative and conjugative elements rather than to other known catabolic plasmids. As far as catabolic functions, the clc element harbored, in addition to the genes for chlorocatechol degradation, a complete functional operon for 2-aminophenol degradation and genes for a putative aromatic compound transport protein and for a multicomponent aromatic ring dioxygenase similar to anthranilate hydroxylase. The genes for catabolic functions were inducible under various conditions, suggesting a network of catabolic pathway induction. For about half of the open reading frames (ORFs) on the clc element, no clear functional prediction could be given, although some indications were found for functions that were similar to plasmid conjugation. The region in which these ORFs were situated displayed a high overall conservation of nucleotide sequence and gene order to genomic regions in other recently completed bacterial genomes or to other genomic islands. Most notably, except for two discrete regions, the clc element was almost 100% identical over the whole length to a chromosomal region in Burkholderia xenovorans LB400. This indicates the dynamic evolution of this type of element and the continued transition between elements with a more pathogenic character and those with catabolic properties.
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the bibliographies on the topic 'Integrative conjugative element' for other source types:
Dissertations / Theses
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography