Dissertations / Theses on the topic 'Insect-plant relationships'
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Crosswhite, F. S., and C. D. Crosswhite. "Editorial - Insect-Plant Relationships." University of Arizona (Tucson, AZ), 1990. http://hdl.handle.net/10150/609111.
Full textGroen, Simon Cornelis. "Manipulation of plant-insect interactions by insect-borne plant viruses." Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.648187.
Full textGuillet, Gabriel. "Ecophysiological importance of phototoxins in plant-insect relationships." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq26120.pdf.
Full textHunt, Matthew. "Effects of environmental change on endophyte-plant-insect relationships." Thesis, University of Oxford, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.275301.
Full textBarrett, Kerry Louise. "Effects of nitrogen deposition on plant/insect herbivore relationships." Thesis, University of Newcastle Upon Tyne, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307936.
Full textLaxton, Emma. "Relationship between leaf traits, insect communities and resource availability." Thesis, Electronic version, 2005. http://hdl.handle.net/1959.14/483.
Full textBibliography: p. 178-203.
Introduction -- Study sites -- Leaf characteristics and resource availability -- Insect herbivory and resource availability -- Insect communities and resource availability -- Influence of resource availability on recovery from herbivory -- Conclusions.
This project used the resource availability hypothesis (Coley et al., 1985) as a framework for investigating the relationship between resource availability (as defined by soil nutrients), leaf traits, insect herbivore damage and insect community structure. According to the hypothesis, plants from low resource environments should be better-defended, have longer leaf lifespans and slower growth rates than plants from higher resource environments. Higher resource plant species are expected to suffer higher levels of herbivory and recover faster from herbivory than low resource plant species (Coley et al. 1985). A corollary to this hypothesis is that plants from higher resource sites should support greater densities of insect herbivores than low resource species. Comparisons between high and low resource sites were made in terms of: (i) leaf traits of mature and immature leaves; (ii) phenology of leaf maturation; (iii) herbivore damage in the field and laboratory; (iv) diversity and abundance of herbivorous insect fauna; and (v) ability to recover from herbivory.
Mode of access: World Wide Web.
243 p. ill., maps
Carey, David Brennan. "Factors determining host plant range in two lycaenid butterflies." Diss., The University of Arizona, 1992. http://hdl.handle.net/10150/185907.
Full textMorrison, Peter D. S. "Host plant variation and population limitation of two introduced insects." Thesis, University of British Columbia, 1986. http://hdl.handle.net/2429/27464.
Full textScience, Faculty of
Zoology, Department of
Graduate
Helson, Julie Elizabeth. "Tropical host plant-insect relationships as guides to medicinally-active plants." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=98723.
Full textSteffan, Shawn Alan. "Biodiversity and fear ecology the cascading effects of species richness and nontrophic interactions /." Pullman, Wash. : Washington State University, 2009. http://www.dissertations.wsu.edu/Dissertations/Spring2009/s_steffan_041709.pdf.
Full textHersch, Erika I. "Evidence for hybridization between three Indian paintbrush species : ecological implications and evolutionary scenarios /." view abstract or download file of text, 2007. http://proquest.umi.com/pqdweb?did=1432774901&sid=1&Fmt=2&clientId=11238&RQT=309&VName=PQD.
Full textTypescript. Includes vita and abstract. Includes bibliographical references (leaves 201-241). Also available for download via the World Wide Web; free to University of Oregon users.
Coetzee, J. H. (Jacobus Hendrik). "Arthropod communities of Proteaceae with special emphasis on plant-insect interactions." Thesis, Stellenbosch : Stellenbosch University, 1989. http://hdl.handle.net/10019.1/66615.
Full textENGLISH ABSTRACT: Arthropod communities of five proteaceous species viz. Protea repens. P. neriifolia. P.cynaroides, Leucospermum cordifolium and Leucadendron /aureolum were studied. A faunal list of the insects was compiled according to the feeding guilds of insects. Six phytophagous guilds were recognized: flower visitors, thrips, endophages, ectophages, sap-suckers, and ants. The guild composition, species packing and seasonal distribution for the free-IMng insects, spiders and other arthropods were investigated on the different plant species. The ants, flower visitors and sap-sucking species found on the plant species, were very similar, but the number of leaf chewing species common to all the plant species was low, suggesting that some species were monophagous. Insect abundance increased during the wet winter months when the plants were in flower. Of the total number of herbivores collected, flower visitors represented 69,5%, leaf feeders 14,5% and sap-suckers 16,1 %. The chewers constituted 60,1% of the total herbivore. biomass. Leaf damage increased with age ; young leaves were practically free of herbivore damage, while older leaves were stm acceptable as food. The degree of insect damage differed amongst the plant species varying from 2% to 15%. The following leaf characteristics were investigated to determine the factors involved in defence against insect herbivory : total phenolic content, protein precipitating ability, cyanogenesis, nitrogen content, leaf toughness, woodiness and fibre content lt appears that the plant species use different mechanisms to protect their leaves. The infructescences of Protea repens were exploited by the larvae of four Coleoptera and four Lepidoptera species. The community structure of these phytophagous insects was determined largely by negative interactions. Various strategies are followed (e.g. niche segregation in time and space) to lessen interspecific competition.
AFRIKAANSE OPSOMMING: 'n Studie van die arthropoocl gemeenskap op vyf proteasoorte, naamlik Protea repens. P. neriffolia. P. cynaroides. Leucospennum cordffolium en Leucadendron laureolum is gedoen. 'n Faunalys van insekte volgens hul voedingsgDdes is saamgestel. Die insekte is in ses fitofage gDdes verdeel naamlik blombesoekers, blaaspootjies, endovoeders, ektovoeders, sapsuiers en miere. Die samestelling van die gDdes, spesie-rangskikking en seisoenale verspreiding is bepaal vir die vrylewende insekte, spinnekoppe en ander arthropode. Die miere, blombesoekers en sapsuierspesies wat op die plantsoorte gevind is, is byna dieselfde, maar verslalle in die blaarvoederspesies is gevind wat 'n aanduiding is dat sommige spesies monofaag mag wees. Gedurende die nat wintermaande toon die insekgetalle 'n toename. Blombesoekers verteenwoordig 69,5%, blaarvoeders 14,5% en sapsuiers 16,1% van die aantal insekherbivore wat versamel is. Die kouers vorm 60,1% van die totale herbivoor biomassa Soos die blare verouder, neem die skade op die blare toe; jong blare toon byna geen skade nie, terwyl ouer blare steeds geskik is vir insekvoedsel. Die insekskade op die blare wissel by die verslallende plante tussen 2% en 15%. Om te bepaal watter blaareienskappe moontlik 'n rol kan speel in blaarverdediging teen insekte is die volgende ondersoek: totale fenoliese inhoud, die vermoe om proteiene te presipiteer, sianogenese. stikstofinhoud, blaartaaiheid, houtagtigheid en veselinhoud. Dit wil voorkom asof die plante verskillende meganismes gebruik om die blare te beskerm. Saadkoppe van Protea repens word deur vier Coleoptera en vier Lepidoptera spesies benut Die gemeenskapstruktuur van die boarders word tot 'n groat mate bepaal deur negatiewe interaksies. Verskeie strategiee word gevolg (bv. nissegregasie in ~ en ruimte) om kompetisie tussen spesies te verminder.
Mercader, Rodrigo J. "Species ranges, host selection, and hybridization how increased hybridization is leading to host use divergence in a polyphagous sibling species pair /." Diss., Connect to online resource - MSU authorized users, 2008.
Find full textTitle from PDF t.p. (viewed on July 17, 2009) Includes bibliographical references (p. 115-126). Also issued in print.
Lorraine, Debrah F. "Oxidation of plant allelochemicals by phytophagous sucking insects." Title page, table of contents and summary only, 1995. http://web4.library.adelaide.edu.au/theses/09PH/09phl876.pdf.
Full textGryj-Rubenstein, Ellen Orli. "Conflicting forces shaping reproductive strategies of plants : florivory and pollination /." Thesis, Connect to this title online; UW restricted, 1999. http://hdl.handle.net/1773/5126.
Full textLandi, Lucia. "Phytoplasma-associated grapevine yellow diseases and their relationships with their plant and insect host." Doctoral thesis, Università Politecnica delle Marche, 2009. http://hdl.handle.net/11566/242361.
Full textMcNett, Gabriel Dion. "Noise and signal transmission properties as agents of selection in the vibrational communication environment." Diss., Columbia, Mo. : University of Missouri-Columbia, 2007. http://hdl.handle.net/10355/4677.
Full textThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on February 25, 2008) Vita. Includes bibliographical references.
Siekmann, Gitta. "Food foraging in adult parasitoid Cotesia rubecula : how sugar sources contribute to survival and reproduction." Title page, contents and abstract only, 2002. http://web4.library.adelaide.edu.au/theses/09PH/09phs5715.pdf.
Full textRoss, Sally Jane. "The phytophagous insect community on the Veld Fig, Ficus Burtt-Davyi Hutch." Thesis, Rhodes University, 1994. http://hdl.handle.net/10962/d1005425.
Full textSimpson, Katrina Lynn Scott. "Interactions between aphids and their host plants under drought stress." Thesis, University of Edinburgh, 2013. http://hdl.handle.net/1842/8294.
Full textHigton, Roger Newell. "Studies in gall induction with special reference to the pontania-salix system." Thesis, University of Oxford, 1991. http://ora.ox.ac.uk/objects/uuid:d8b9b0c4-8734-46b4-a5b6-f8dd01290954.
Full textClark, Maxwell Maitland. "The host-searching behaviour of coccophagus atratus compere (Aphalinidae: hymenoptera)." Thesis, Rhodes University, 1985. http://hdl.handle.net/10962/d1004923.
Full textTurna, Michael T. "Extraction of a phagostimulant and classification of the feeding recognition template for larvae of the moth Malacosoma americanum." Diss., Online access via UMI:, 2008.
Find full textUnderwood, Angela Marie Spano Folkerts Debbie R. "Larvae of Sarcophagidae (Insecta Diptera) and their relationship with the pitcher plants (Sarraceniaceae: Sarracenia) of southeastern U.S. bogs /." Auburn, Ala, 2009. http://hdl.handle.net/10415/1889.
Full textMajorczyk, Alexis M. "Chemical Defense Mechanisms of Arabidopsis thaliana Against Insect Herbivory: The Role of Glucosinolate Hydrolysis Products." Bowling Green, Ohio : Bowling Green State University, 2009. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=bgsu1249513273.
Full textRebe, Makhosandile. "Lepidoptera stem borers on cultivated and wild host plants : implications of insect-plant interactions for pest management." Thesis, University of Pretoria, 2002. http://hdl.handle.net/2263/28637.
Full textPuterbaugh, Mary Norris. "Alpine plant-ant interactions /." free to MU campus, to others for purchase, 1997. http://wwwlib.umi.com/cr/mo/fullcit?p9841329.
Full textMay, Bronwen. "Investigations into insect-induced plant responses of water hyacinth (Eichhornia crassipes (Mart.) Solms-Laub.) (Pontederiaceae)." Thesis, Rhodes University, 2015. http://hdl.handle.net/10962/d1018906.
Full textBallantyne, Gavin. "Ants as flower visitors : floral ant-repellence and the impact of ant scent-marks on pollinator behaviour." Thesis, University of St Andrews, 2011. http://hdl.handle.net/10023/2535.
Full textCurrano, Ellen Diane. "Variations in insect herbivory on angiosperm leaves through the late Paleocene and early Eocene in the Bighorn Basin, Wyoming, USA /." View online, 2008. http://etda.libraries.psu.edu/theses/approved/WorldWideFiles/ETD-2863/Thesis_Currano_final.pdf.
Full textHasenbank, Marc. "Egg laying on patchy resources and the importance of spatial scale : a thesis submitted to the Victoria University of Wellington in fulfilment of the requirements for the degree of Doctor of Philosophy in Ecology & Biodiversity /." ResearchArchive@Victoria e-Thesis, 2010. http://hdl.handle.net/10063/1152.
Full textAkbulut, Süleyman. "Effect of Bursaphelenchus xylophilus (Nematod:Aphelenchoididae) fourth stage dispersal of juveniles and log seasonality on life processes of Monochamus carolinensis (Coleoptera:Cerambycidae) /." free to MU campus, to others for purchase, 1998. http://wwwlib.umi.com/cr/mo/fullcit?p9901211.
Full textHannunen, Salla Ekbom Barbara. "Trivial movements and redistribution of polyphagous insect herbivores in heterogeneous vegetation /." Uppsala : Swedish University of Agricultural Sciences, 2003. http://diss-epsilon.slu.se/archive/00000167/.
Full textThesis documentation sheet inserted. Includes appendix of four papers and manuscripts, two co-authored with Barbara Ekbom. Includes bibliographical references. Also partially issued electronically via World Wide Web in PDF format; online version lacks appendix of papers.
Zhang, Qing-He. "Olfactory recognition and behavioural avoidance of angiosperm non-host volatiles by conifer bark beetles /." Alnarp : Swedish Univ. of Agricultural Sciences (Sveriges lntbruksuniv.), 2001. http://epsilon.slu.se/avh/2001/91-576-5789-0.pdf.
Full textCunnold, Helen Elizabeth. "Distinguishing pollination from visitation : the value of a pollinator effectiveness and pollinator importance network." Thesis, University of St Andrews, 2018. http://hdl.handle.net/10023/16121.
Full textBai, Xiaodong. "Insect transmitted plant pathogenic mollicutes, Spiroplasma kunkelii and aster yellows witches' broom phytoplasma from structural genomics to functional genomics /." Connect to this title online, 2004. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1101752677.
Full textTitle from first page of PDF file. Document formatted into pages; contains xvii, 232 p.; also includes graphics (some col.). Includes bibliographical references (p. 206-232).
Stout, Benjamin Mortimer. "Effects of forest disturbance on shredder production in headwater streams." Diss., Virginia Tech, 1990. http://hdl.handle.net/10919/39796.
Full textBinder, Bradley Fletcher. "The chemistry and biology of insect growth regulators from plants in the genus Nama (Hydrophyllaceae)." Diss., The University of Arizona, 1989. http://hdl.handle.net/10150/184795.
Full textMuwanga-Zake, Johnnie Wycliffe Frank. "Insect herbivores associated with Senecio pterophorus and Senecio inaequidens at Butterworth, South Africa." Thesis, Rhodes University, 1995. http://hdl.handle.net/10962/d1005402.
Full textBottcher, Claudia. "Interações entre Pachycondyla striata e Odontomachus chelifer (Formicidae, Ponerinae) e diasporos em tres fisionomias florestais da Mata Atlantica." [s.n.], 2006. http://repositorio.unicamp.br/jspui/handle/REPOSIP/316275.
Full textDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: O presente estudo investigou a variação espacial nas interações envolvendo os poneríneos Pachycondyla striata e Odontomachus chelifer e diásporos não-mirmecocóricos. O estudo foi desenvolvido em três formações florestais contínuas na Floresta Atlântica: Mata de Restinga (2-3 m acima do nível do mar), Floresta de Planície (5-30 m acima do nível do mar) e Floresta de Encosta (200-250 m acima do nível do mar) no Parque Estadual da Ilha do Cardoso, Cananéia, SP. Investiguei como tais interações variam espacialmente comparando os padrões das interações formiga-diásporo, e o estabelecimento, crescimento e sobrevivência inicial de plântulas nas nossas três áreas de estudo. Possíveis causas delineando tais padrões foram examinadas (quantidade de serapilheira, penetrabilidade do solo, biomassa de frutos e artrópodes, abundância e preferência dos poneríneos). Operárias solitárias de P. striata e O. chelifer transportam diásporos para seus ninhos de maneira similar nas três áreas de estudo. Contudo, diásporos ricos em lipídeos são preferencialmente removidos por ambas as espécies. A baixa disponibilidade de artrópodes na serapilheira pode ter afetado o padrão observado, pois os poneríneos são primariamente carnívoros. Plântulas e juvenis foram mais freqüentes perto dos ninhos dessas formigas que em áreas controle sem tais ninhos em mata de restinga e em floresta de planície. O recrutamento das plântulas em ninhos de P. striata e O. chelifer foi maior que em áreas controle, sem ninho, em mata de restinga e floresta de planície, mas o mesmo não ocorreu em floresta de encosta. Este padrão pode estar relacionado às propriedades e penetrabilidade do solo, diferente para cada uma das áreas estudadas. Embora as interações entre P. striata e O. chelifer e diásporos não-mirmecocóricos ocorreram nos três ambientes estudados e envolvam uma variedade de espécies de plantas, há diferenças importantes entre as áreas. Os resultados indicaram que a interação formiga-diásporo varia no espaço com importantes conseqüências para as plantas envolvidas
Abstract: The present study investigated the spatial variation in interactions involving the ponerine ants Pachycondyla striata and Odontomachus chelifer and nonmyrmecochorous diaspores. The study was carried out at the three continuous forests formations in the Brazilian Atlantic forest: Restinga forest (2-3 m above sea level), Lowland forest (05-30 m above sea level), Hillside forest (200-250 m above sea forest) at Parque Estadual da Ilha do Cardoso, Cananéia, SP. We examined how these interactions vary spatially by comparing the patterns of selected ant-diaspore interactions and establishment, growth and early survival of seedlings occurring at our three main study areas. Possible causes underlying such patterns were examined (leaf litter, soil penetrability, fruits and arthropods biomass, ponerines abundance and preference). Individual workers of P. striata and O. chelifer transport single diaspores to their nest by similar way across the three study areas. However the lipid rich diaspores were preferentially removed by both ponerine species. The low availability of leaf litter arthropods may have affected the observed pattern whereas the ponerines are primarily carnivorous. Seedlings and juveniles were more frequent close to ponerine nests than in control areas without such nests in restinga and lowland forests. The seedling recruitment in nests of P. striata and O. chelifer was greater than in control areas in restinga and lowland forests but not in hillside forest. Seedlings grew better only in nests of O. chelifer in lowland forest. This pattern may be related to soil properties and penetrability that are different between the areas. Although the interactions between P. striata and O. chelifer and nonmyrmecochorous diaspores occur at the three study areas and involve a variety of plant species at all places, there are important differences between the sites. The results indicate that ant-seed interaction vary in space with important consequences for the plants involved.
Mestrado
Mestre em Ecologia
Chorbadjian, Rodrigo A. "Phenotypic variation in host quality of pines for the European pine sawfly (Neodiprion sertifer)." Columbus, Ohio : Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1230736665.
Full textHunter, Alison F. (Alison Fiona). "Synchrony with host leaf emergence as a component of population dynamics in lepidopteran folivores." Thesis, McGill University, 1991. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=70287.
Full textPelletier, Georges. "Plants and arthropods associated with orthopteroids in abandoned fields of Southern Quebec." Thesis, McGill University, 1986. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=65377.
Full textKersch, Monica Frank. "Poda experimental de Chromolaena pungens (Asteraceae) e seus efeitos multitroficos." [s.n.], 2007. http://repositorio.unicamp.br/jspui/handle/REPOSIP/316239.
Full textDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: Perturbações naturais ou antrópicas podem induzir mudanças fenotípicas nas plantas, que por sua vez podem influenciar herbívoros, predadores e polinizadores. Fatores abióticos, tais como nutrientes também podem influenciar a recuperação das plantas. Neste estudo investigamos o efeito de poda experimental em artrópodos associados a plantas rebrotadas em populações naturais de Chromolaena pungens (Asteraceae: Eupatoriae), uma planta subarbustiva, perene e nativa de cerrado. Os objetivos deste trabalho foram avaliar como o rebrote após corte raso ou poda parcial e o suprimento de nutrientes influenciam: (i) o crescimento vegetativo, (ii) a reprodução e os caracteres florais, (iii) a abundância de galhas, insetos folívoros, insetos sugadores e seus predadores; (iv) a comunidade de insetos endófagos associados e a incidência de seus parasitóides. O experimento seguiu um desenho de dois fatores em blocos, onde foram manipulados corte (controle, corte parcial e corte raso) e nutrientes (sem adição de nutrientes e com adição de nutrientes). Plantas cortadas na base foram menores, produziram menos folhas e capítulos, tiveram sua fenologia retardada em um mês em comparação com plantas controle ou de corte parcial. No entanto, a taxa de crescimento, a área foliar e o tamanho dos capítulos foram maiores em plantas submetidas a corte raso. Tais plantas sofreram maiores níveis de herbivoria foliar e destruição de sementes. Além disso, a abundância, a riqueza, a diversidade de espécies de endófagos associados a capítulos de plantas cortadas foram maiores do que em plantas controle ou parcialmente cortadas. Em conseqüência, a composição de espécies endófagas diferiu entre plantas submetidas a diferentes tipos de corte. Por isso, os resultados deste estudo são relevantes para o entendimento de interações inseto-planta em ambientes que sofrem perturbações naturais ou antrópicas recorrentes
Abstract: Natural or human-induced disturbances may promote plant phenotypic modifications, developmental and phenological changes which, in turn, may influence herbivores, predators and pollinators. Abiotic factors, especially nutrient availability, may also influence plant recovery. In this study, we evaluated plant regrowth and arthropod responses to experimental clipping plants in natural populations of Chromolaena pungens (Asteraceae: Eupatoriae), a perennial, native shrub in the Brazilian Cerrado. In particular, we assess how resprouting after severe or partial damage and nutrients influence: (i) vegetative regrowth; (ii) reproductive effort and floral traits; (iii) the abundance of galling, leaf-chewing and sap-sucking insects and their predaceous arthropods; (iv) endophagous insects and their parasitoids. The experiment followed a two-factor randomized block design, respectivelly clipping (control, partial clipping and basal clipping) and nutrients (no nutrient added and with nutrients added). Basally clipped plants were smaller, produced fewer leaves and flowerheads than partially clipped and control plants, moreover heir floral phenology was delayed by one month. However, the relative elongation rate, leaf area and flowerhead size were greater in basally clipped plants. These plants also suffered higher leaf herbivory and seed destruction. Moreover, the abundance, species richness and abundance of flowerhead endophages were higher in basally clipped plants than in the other treatments. Not surprisingly, species composition differed among clipping treatments. For this reason, these findings contribute to the knowledge of insect-plant interactions in recurringly disturbed habitats
Mestrado
Ecologia
Mestre em Ecologia
Robertson, Hamish Gibson. "The ecology of Cactoblastis cactorum (Berg) (Lepidoptera : phycitidae) in relation to its effectiveness as a biological control agent of prickly pear and jointed cactus in South Africa." Thesis, Rhodes University, 1985. http://hdl.handle.net/10962/d1005356.
Full textZachariades, Costas. "Complex interactions involving the Cape fig, Ficus sur Forsskål, and its associated insects." Thesis, Rhodes University, 1995. http://hdl.handle.net/10962/d1005338.
Full textDe, Sibio Paula Roberta [UNESP]. "Efeitos tipo bottom-up e top-down em um sistema tritrófico formado por Ricinus communis L. (Euphorbiaceae), Spodoptera frugiperda (Lepidoptera: Noctuidae) e seus inimigos naturais." Universidade Estadual Paulista (UNESP), 2012. http://hdl.handle.net/11449/104006.
Full textCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Not available
Oliveira, Caio Fernando Ramalho de 1987. "Aspectos funcionais e estruturais do inibidor de tripsina de Entada acaciifolia." [s.n.], 2011. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314778.
Full textDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: Diversas pesquisas buscam candidatos para o controle alternativo de pragas agrícolas, uma vez conhecidos os malefícios oriundos do emprego de inseticidas químicos nas lavouras. Neste trabalho, um inibidor de tripsina isolado de sementes de Entada acaciifolia (Mimosoideae) - (EATI) foi purificado e caracterizado sob o ponto de vista funcional e estrutural. O inibidor mostrou-se estável a variações térmicas e de pH, alem de apresentar sua atividade inibitória inalterada tanto na forma oxidada quanto na forma reduzida com 100 mM de DTT. Quando submetido a cromatografia liquida de alta eficiência em coluna C-18, EATI foi resolvido em 4 picos, indicando a presença de 4 isoformas, sendo que a isoforma majoritária apresentou uma massa acurada de 19.725 Daltons, revelada através de espectrometria de massa MALDI-TOF. Outras características como a estequiometria de inibição, a constante de inibição (Ki), a analise da composição global de aminoácidos e o sequenciamento N-terminal permitiram classificar EATI como membro da família Kunitz de inibidores de serinoprotease. Estudos de dicroísmo circular revelaram um alto conteúdo de fohas-? e estruturas não ordenadas alem da aparente ausência de ?-helices, padrão comum a esta classe de proteínas. A incubação em 1 mM de DTT resultou em perturbações em sua cadeia polipeptídica, corroborando os ensaios in vitro. EATI foi resistente a desnaturação por uréia, característica relatada para outros inibidores vegetais. O inibidor apresentou uma interessante atividade inibitória in vitro contra as proteases de diversos insetos-praga. De acordo com estes resultados, apontamos EATI como uma ferramenta promissora no combate a pragas agrícolas
Abstract: Several studies seeking alternative candidates for the control of agricultural pests, once known harm from the use of chemical insecticides in crops. In this work, a trypsin inhibitor isolated from Entada acaciifolia seeds (Mimosoideae) - (EATI) was purified and characterized from the point of view unctional and structural. The inhibitor was stable to thermal heat and pH range, in addition to its inhibitory activity unchanged in both the oxidized and in reduced form with 100 mM DTT. When subjected to high performance liquid chromatography on C-18 column, EATI was resolved in four peaks, indicating the presence of four isoforms, with the major isoform showing an accurate mass of 19,725 Daltons, as revealed by MALDI-TOF mass spectrometry. Other characteristic such as stoichiometry of inhibition, the dissociation constant (Ki), the analysis of global amino acid composition and N-terminal sequencing allowed to classify EATI as member of the Kunitz family of serine protease inhibitors. Circular dichroism studies revealed a high content of ?- sheets and unordered structures beyond the apparent absence of ?-helix, typical of this class of proteins. Incubation in 1 mM DTT resulted in small perturbations in their polypeptide chain, corroborate the in vitro assays. EATI was resistant to denaturation by urea, a characteristic reported for other plant inhibitors. The inhibitor presented an interesting inhibitory activity in vitro against the proteases of several insect pests. According to these results, we focused EATI as a promising tool in the fight against agricultural pests
Mestrado
Bioquimica
Mestre em Biologia Funcional e Molecular
De, Sibio Paula Roberta. "Efeitos tipo bottom-up e top-down em um sistema tritrófico formado por Ricinus communis L. (Euphorbiaceae), Spodoptera frugiperda (Lepidoptera: Noctuidae) e seus inimigos naturais /." Botucatu, 2012. http://hdl.handle.net/11449/104006.
Full textBanca: Wesley Augusto Conde Godoy
Banca: Lucas Del Bianco Faria
Banca: Julianne Milleo
Banca: Carlos Frederico Wilcken
Resumo: Não disponível
Abstract: Not available
Doutor
Pereira, Marcela Fernandes. "Custos e beneficios da visita de formigas aos nectarios extraflorais de Crotalaria pallida (Fabaceae)." [s.n.], 2008. http://repositorio.unicamp.br/jspui/handle/REPOSIP/316372.
Full textDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: Formigas podem exercer um efeito positivo ou negativo na aptidão das plantas com as quais inferagem, dependendo de suas relações com herbívoros, polinizadores e dispersores de sementes. Uma notável característica das interações entre formigas e plantas é a alta freqüência com a qual essas interações são mutualísticas. O suposto beneficio dessas interações é que a aptidão da planta pode ser aumentada indiretamente, já que uma redução na taxa de herbivoria significa uma maior produção de sementes, ou diretamente, através de redução da predação de sementes. Mas será que as plantas realmente se beneficiam da presença dos insetos que atraem?Apesar de alguns estudos terem encontrado beneficios para a planta, há aqueles que indicam o oposto, como os casos em que polinizadores e dispersores de sémentes evitam flores e frutosdevido à presença de formigas. O legado evolutivo das interações entre plantas, herbívoros e formigas é o aparecimento de novas estruturas em plantas. Crotalaria pallida é uma planta muito visitada por formigas devido à presença de nectários extraflorais (NEFs), glândulas produtoras de açúcar sem relação direta com polinização. Superficialmente, pode parecer que as conseqüências para a planta das suas relações com seus herbívoros sejam necessariamente contrárias às conseqüências para o herbívoro. Por outro lado, uma observação mais.cautélosa revela que alguns mecanismos que produzem efeitos negativos no herbívoro não levam automaticamente a um beneficio da planta. Sendo assim, este estudo visa avaliar a relação custo-beneficio da visitação por formigas em C. pallida através de sua aptidão na presença e ausência de formigas, assim como de observações de campo de interações destas plantas com vespas. As taxas de ganho de biomassa e de herbivoria, assim como a produção de flores, vagens e sementes, bem como o peso das sementes não diferiram estatisticamente entre indivíduos de C. pallida com e sem formigas. Por outro lado, o ataque às vagens foi significativamente menor nos indivíduos de C. allida em que as formigas foram excluídas, e a sobrevivência destas plantas foi maior, em comparação com as plantas que foram visitadas normalmente por formigas. Um experimento de campo indicou que a predação de larvas não parece ser influenciada pela presença de vespas, mas apenas por formigas. Por outro lado, durante observações de campo todas as vespas que visitaram racemos de C. pallida foram influenciadas pela Presença de formigas, sendo que algumas reduziram seu tempo de visitação e outras deixaram o racemo após o encontro com formigas. Assim, os resultados obtidos através dos experimentos e observações de campo sugerem que as formigas não afetam a aptidão de C. pallida, pelo menos não nas condições encontradas na área de estudo deste trabalho
Abstract: Ants may have a positive or negative effect on the fitness of the plants with which they interact, depending on its relationship with herbivores, pollinators and seed dispersers. A remarkable characteristic of the interactions between ants and plants is the high frequency with which they are mutualistic. The supposed benefit of these interactions is that the plant fitness may be enhanced indirectly, since herbivory reduction means larger seed production, or direct1y, by reduction on seed predation. But do plants really benefit from the presence of the insects they attract? Despite some studies have found benefits to the plant, there are some that indicate the opposite, like those cases in which pollinators and seed dispersers avoid flowers and fruits due to ant attendance. The evolutive legacy of the interactions between plants, herbivores and ants is the appearance of new plant structures. Crotalaria pallida is a plant highly attended by ants because of its extrafloral nectaries (EFNs), su gar-producing glands with no pollination relationship, located on the base of each flower or pod. Superficially, it may seem that the consequences of the plant relationship with its herbivores are necessar~ly opposed to the consequences to the herbivore. On the other hand, a cautious observation reveals that some mechanisms ;that produce negative effects on the herbivore not automatically lead to a benefit for the plant. Therefor, this work aim to evaluate the magnitude of the costs and benefits of ant attendance to the EFN s of C. pallida on the protection against phytophagous insects, by estimating its fitness in the presence and absence of ants, as well as using field observations of the interactions of these plants with wasps. The rates of biomass gain and herbivory, likewise the flower, pod and seed production, and also seed weight didn't differ significantly between ant-attended and non ant-attended C. pallida plants. As opposed to this, the pod attack was significant1y lower in C. pallida plants not attended by ants, and the survival of these plants was higher, in comparison to the ant-attended plants. A field experiment indicated that larvae predation don't seem to be in:t1uenced by the presence of wasps, but only by ant attendance. On the other hand, during field observations all the wasps visiting C. pallida pods were affected by ant attendance, and some of them reduced its visitation time while others left the pod after meeting an ant. 80, the results we got with the experiments and field observations suggest that the ants don't affect C. pallida fitness, at least not under the conditions found in the area where this work took place
Mestrado
Mestre em Ecologia