Academic literature on the topic 'Inducibility'

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Journal articles on the topic "Inducibility"

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Dossou-Olory, Audace A. V., and Stephan Wagner. "Inducibility of topological trees." Quaestiones Mathematicae 42, no. 6 (August 8, 2018): 749–64. http://dx.doi.org/10.2989/16073606.2018.1497725.

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Choi, Ilkyoo, Bernard Lidický, and Florian Pfender. "Inducibility of directed paths." Discrete Mathematics 343, no. 10 (October 2020): 112015. http://dx.doi.org/10.1016/j.disc.2020.112015.

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Czabarka, Éva, Audace A. V. Dossou-Olory, László A. Székely, and Stephan Wagner. "Inducibility of d-ary trees." Discrete Mathematics 343, no. 2 (February 2020): 111671. http://dx.doi.org/10.1016/j.disc.2019.111671.

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Hefetz, Dan, and Mykhaylo Tyomkyn. "On the inducibility of cycles." Journal of Combinatorial Theory, Series B 133 (November 2018): 243–58. http://dx.doi.org/10.1016/j.jctb.2018.04.008.

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RAMIREZ, J., F. INNOCENTI, B. KOMOROSKI, S. MIRKOV, A. YODERGRABER, D. FACKENTHAL, S. DAS, E. SCHUETZ, S. STROM, and M. RATAIN. "Genetic determinants of UGT1A1 inducibility." Clinical Pharmacology & Therapeutics 77, no. 2 (February 2005): P25. http://dx.doi.org/10.1016/j.clpt.2004.11.096.

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Hefetz, Dan, and Mykhaylo Tyomkyn. "On the inducibility of cycles." Electronic Notes in Discrete Mathematics 61 (August 2017): 593–99. http://dx.doi.org/10.1016/j.endm.2017.07.012.

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Messier, H., J. Ratanavongsiri, T. Fuller, S. Mangal, P. Kilgannon, R. Fotedar, and A. Fotedar. "Mapping of an inducible element in the T cell receptor V beta 2 promoter." Journal of Immunology 149, no. 6 (September 15, 1992): 1980–86. http://dx.doi.org/10.4049/jimmunol.149.6.1980.

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Abstract The murine V beta 2 promoter was analyzed for an element regulating phorbol ester inducibility of the TCR beta chain gene. In transient expression analysis of 5' nested deleted fragments of the V beta 2 promoter, the TPA-inducible element mapped between -85 and -42. The -85 to -62 oligo conferred 12-0-tetradecanoylphorbol-13-acetate (TPA) inducibility to the heterologous TPA-uninducible thymidine kinase promoter. The -85 to -62 region contained an AP-1 site (-85 to -72) and inverted repeat motif (-72 to -62). The AP-1 site required the 3' flanking inverted repeat region for conferring optimal inducibility. In vitro transcribed and translated jun/fos heterodimers bind to the V beta 2 AP-1 motif with a 16-fold lower affinity as compared to the collagenase AP-1 motif. This explains the inability of the V beta 2 AP-1 motif to confer optimal TPA inducibility by itself. The affinity of jun/fos heterodimers for the V beta 2 AP-1 motif was not increased by the presence in cis of the inverted repeat motif. The 3' flanking inverted repeat binds the ets transactivator but not jun/fos heterodimers. The demonstrated cooperativity between the AP-1 and the 3' flanking sequence to confer TPA inducibility can thus be explained by the individual contributions of jun/fos and ets transactivators.
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Uliana Trentin, Henrique, Grigorii Batîru, Ursula Karoline Frei, Somak Dutta, and Thomas Lübberstedt. "Investigating the Effect of the Interaction of Maize Inducer and Donor Backgrounds on Haploid Induction Rates." Plants 11, no. 12 (June 7, 2022): 1527. http://dx.doi.org/10.3390/plants11121527.

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Doubled haploid technology is a feasible, fast, and cost-efficient way of producing completely homozygous lines in maize. Many factors contribute to the success of this system including the haploid induction rate (HIR) of inducer lines, the inducibility of donor background, and environmental conditions. Sixteen inducer lines were tested on eight different genetic backgrounds of five categories in different environments for the HIR to determine possible interaction specificity. The HIR was assessed using the R1-nj phenotype and corrected using the red root marker or using a gold-standard test that uses plant traits. RWS and Mo-17-derived inducers showed higher average induction rates and the commercial dent hybrid background showed higher inducibility. In contrast, sweet corn and flint backgrounds had a relatively lower inducibility, while non-stiff stalk and stiff stalk backgrounds showed intermediate inducibility. For the poor-performing donors (sweet corn and flint), there was no difference in the HIR among the inducers. Anthocyanin inhibitor genes in such donors were assumed to have increased the misclassification rate in the F1 fraction and, hence, result in a lower HIR.
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MATSUO, YOSHINORI, and TSUNEYUKI YAMAZAKI. "Genetic factors on the second and third chromosomes responsible for the variation of amylase activity and inducibility in Drosophila melanogaster." Genetical Research 70, no. 2 (October 1997): 97–103. http://dx.doi.org/10.1017/s0016672397002887.

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Using second- or third-chromosome substitution lines of Drosophila melanogaster, the genetic variation of inducibility and amylase specific activities in three media (starch, normal and glucose) were investigated. Genetic factors on both the second and third chromosomes were responsible for the variation in amylase specific activity and inducibility. In glucose medium, the genetic variance of amylase specific activity estimated for the second-chromosome substitution lines was larger than that for the third-chromosome substitution lines; however, for starch medium and inducibility, the variance was larger for the third-chromosome substitution lines. High correlations for the second-chromosome substitution lines and low correlations for the third-chromosome substitution lines were observed for amylase specific activities in different media. These results suggest that the genetic factor(s) responsible for inducibility or amylase activity variation in an induced medium such as starch should be on the third chromosome and those in the non-induced medium such as glucose should be on the second chromosome. The functional roles of the factors on the second and third chromosomes would be the repression and induction of amylase, respectively.
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Massa, P. T., R. Brinkmann, and V. ter Meulen. "Inducibility of Ia antigen on astrocytes by murine coronavirus JHM is rat strain dependent." Journal of Experimental Medicine 166, no. 1 (July 1, 1987): 259–64. http://dx.doi.org/10.1084/jem.166.1.259.

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Inducibility of Ia molecules on cultivated astrocytes by JHM virus correlates with demyelinating disease susceptibility of animals from which these astrocytes are derived. On the contrary, class I induction of both astrocytes and oligodendrocytes occurs as a consequence of normal cultivation procedures in both susceptible and resistant strains. Increased expression of class I antigens on rat astrocytes and oligodendrocytes is not related to JHM viral infection as it is in the mouse. These data indicate that strain differences in Ia inducibility, rather than inducibility of class I antigens, by JHM virus may explain higher levels of T cell-mediated damage to myelin during infection in susceptible rat strains compared with resistant strains.
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Dissertations / Theses on the topic "Inducibility"

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Savory, Richard. "PPAR#alpha# : inducibility and species differences in expression." Thesis, University of Nottingham, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.337248.

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Krenek, Sascha, Martin Schlegel, and Thomas U. Berendonk. "Convergent evolution of heat-inducibility during subfunctionalization of the Hsp70 gene family." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2013. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-126934.

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Background: Heat-shock proteins of the 70 kDa family (Hsp70s) are essential chaperones required for key cellular functions. In eukaryotes, four subfamilies can be distinguished according to their function and localisation in different cellular compartments: cytosol, endoplasmic reticulum, mitochondria and chloroplasts. Generally, multiple cytosol-type Hsp70s can be found in metazoans that show either constitutive expression and/or stress-inducibility, arguing for the evolution of different tasks and functions. Information about the hsp70 copy number and diversity in microbial eukaryotes is, however, scarce, and detailed knowledge about the differential gene expression in most protists is lacking. Therefore, we have characterised the Hsp70 gene family of Paramecium caudatum to gain insight into the evolution and differential heat stress response of the distinct family members in protists and to investigate the diversification of eukaryotic hsp70s focusing on the evolution of heat-inducibility. Results: Eleven putative hsp70 genes could be detected in P. caudatum comprising homologs of three major Hsp70-subfamilies. Phylogenetic analyses revealed five evolutionarily distinct Hsp70-groups, each with a closer relationship to orthologous sequences of Paramecium tetraurelia than to another P. caudatum Hsp70-group. These highly diverse, paralogous groups resulted from duplications preceding Paramecium speciation, underwent divergent evolution and were subject to purifying selection. Heat-shock treatments were performed to test for differential expression patterns among the five Hsp70-groups as well as for a functional conservation within Paramecium. These treatments induced exceptionally high mRNA up-regulations in one cytosolic group with a low basal expression, indicative for the major heat inducible hsp70s. All other groups showed comparatively high basal expression levels and moderate heat-inducibility, signifying constitutively expressed genes. Comparative EST analyses for P. tetraurelia hsp70s unveiled a corresponding expression pattern, which supports a functionally conserved evolution of the Hsp70 gene family in Paramecium. Conclusions: Our analyses suggest an independent evolution of the heat-inducible cytosol-type hsp70s in Paramecium and in its close relative Tetrahymena, as well as within higher eukaryotes. This result indicates convergent evolution during hsp70 subfunctionalization and implies that heat-inducibility evolved several times during the course of eukaryotic evolution.
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Wolfgang, Curt Douglas. "A study of the biological function of ATF3 : stress inducibility, target promoters and functional consequences /." The Ohio State University, 2000. http://rave.ohiolink.edu/etdc/view?acc_num=osu148819244742789.

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Takahashi, Akihisa. "Different inducibility of radiation-or heat-induced p53-dependent apoptosis after acute or chronic irradiation in human cultured squamous cell carcinoma cells." 京都大学 (Kyoto University), 2001. http://hdl.handle.net/2433/150903.

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Sharma, Kalpana [Verfasser]. "Genetic variation of and environmental effects on inducibility of resistance in tomatoes (Solanum lycopersicum L.) to Phytophthora infestans (Mont) de Bary / Kalpana Sharma." Kassel : Universitätsbibliothek Kassel, 2010. http://d-nb.info/1009299522/34.

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Angendohr, Stephan Jürgen Heinrich [Verfasser]. "Bedeutung des intrinsischen kardialen autonomen Nervensystems für die ventrikuläre Elektrophysiologie und Arrhythmieinduzierbarkeit : Impact of the intrinsic cardiac autonomic nervous system on ventricular arrhythmogenesis and inducibility of ventricular arrhythmia / Stephan Jürgen Heinrich Angendohr." Hamburg : Staats- und Universitätsbibliothek Hamburg Carl von Ossietzky, 2020. http://d-nb.info/122108481X/34.

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"Inducibility and overexpression studies of antiquitin in HEK293 and HepG2 cells." 2005. http://library.cuhk.edu.hk/record=b5896393.

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Abstract:
Wong Wei-yan.
Thesis (M.Phil.)--Chinese University of Hong Kong, 2005.
Includes bibliographical references (leaves 221-242).
Abstracts in English and Chinese.
Thesis committee --- p.i
Declaration --- p.ii
Acknowledgements --- p.iii
Abstract in Chinese --- p.iv
Abstract in English --- p.vi
List of abbreviations --- p.viii
List of figures --- p.xi
List of tables --- p.xv
Content: --- p.xvi
General introduction --- p.1
Aldehyde dehydrogenase superfamily --- p.3
Background of antiquitin --- p.5
Plant antiqutins (ALDH7B) --- p.5
Animal antiquitins (ALDH7A) --- p.8
Human antiquitin information on NCBI --- p.14
Rationale of studying the inducibility of annquitin and overexpression of it in HEK293 and HepG2 cells --- p.16
Flowchart 1 Procedure of antiquitin expression studies in the HEK293 and HepG2 cells under stress --- p.19
Flowchart 2 Procedure to study antiquitin expression in the HEK293 and HepG2 cells after in silico promoter search --- p.20
Flowchart 3 Procedure to study antiquitin overexpressed HEK293 and HepG2 cells --- p.21
Chapter Chapter 1 --- Inducibility of antiquitin in the HEK293 and HepG2 cells under hyperosmotic stress
Chapter 1.1 --- Introduction --- p.22
Chapter 1.1.1 --- Cellular response to hyperosmotic stress --- p.22
Chapter 1.1.2 --- Methods to study the responses of cells under hyperosmotic stress --- p.24
Chapter 1.2 --- Materials --- p.26
Chapter 1.2.1 --- Cell culture media --- p.26
Chapter 1.2.2 --- Buffers for RNA use --- p.26
Chapter 1.2.3 --- Buffers for DNA use --- p.27
Chapter 1.2.4 --- Other chemicals --- p.27
Chapter 1.3 --- Methods --- p.28
Chapter 1.3.1 --- Culture of HEK293 and HepG2 cells --- p.28
Chapter 1.3.2 --- Hyperosmotic stress on HEK293 and HepG2 cells --- p.29
Chapter 1.3.3 --- MTT assay --- p.29
Chapter 1.3.4 --- Total RNA extraction --- p.30
Chapter 1.3.5 --- Reverse transcription polymerase chain reaction (RT-PCR) --- p.30
Chapter 1.3.6 --- Polymerase chain reaction (PCR) --- p.31
Chapter 1.3.7 --- Quantification of PCR products --- p.31
Chapter 1.3.8 --- Statistical analysis --- p.33
Chapter 1.4 --- Results --- p.34
Chapter 1.4.1 --- Viability of HEK293 and HepG2 cells under hyperosmotic stress --- p.34
Chapter 1.4.2 --- Validation of RNA quality --- p.34
Chapter 1.4.3 --- Validation and determination of PCR conditions --- p.40
Chapter 1.4.4 --- Inducibility of antiquitin in HEK293 cells under hyperosmotic stress
Chapter 1.4.5 --- Inducibility of antiquitin in HepG2 cells under hyperosmotic stress --- p.43
Chapter 1.4.6 --- Inducibility of aldose reductase under hyperosmotic stress --- p.43
Chapter Chapter 2 --- "In silico studies of human antiquitin promoter, genomics sequences and open reading frame" --- p.54
Chapter 2.1 --- Introduction --- p.54
Chapter 2.1.1 --- Eukaryotic promoters --- p.55
Chapter 2.1.2 --- Key events in transcriptional initiation --- p.55
Chapter 2.1.3 --- Alternative splicing of mRNA --- p.57
Chapter 2.1.4 --- Bipartite nuclear localization signal (NLS) --- p.57
Chapter 2.2 --- Methods --- p.60
Chapter 2.2.1 --- Putative promoter studies of human antiquitin --- p.60
Chapter 2.2.2 --- Putative promoter studies of Arabidopsis thaliana antiquitin --- p.60
Chapter 2.2.3 --- Analysis for the alternative splicing of human antiquitin mRNA --- p.60
Chapter 2.2.4 --- Analysis for the nuclear localization signal (NLS) of human antiquitin amino acid sequence --- p.61
Chapter 2.2.5 --- Nucleotide / amino acid sequence analyses --- p.61
Chapter 2.3 --- Results --- p.62
Chapter 2.3.1 --- Computer search for the putative cis-acting elements on human antiquitin promoter --- p.62
Chapter 2.3.2 --- Comparison of cis-acting elements found on human antiquitin promoter with those on Arabidopsis thaliana antiquitin promoter --- p.62
Chapter 2.3.3 --- Possibilities of alternative splicing isoforms of human antiquitin
Chapter 2.3.4 --- Possibilities of bipartite nuclear localization signals on human antiquitin protein --- p.83
Chapter Chapter 3 --- Overexpression of antiquitin in HEK293 and HepG2 cells and their characterization
Chapter 3.1 --- Introduction --- p.86
Chapter 3.1.1 --- Cell cycle of a human somatic cell --- p.88
Chapter 3.1.2 --- Detection of changes in the transcriptome --- p.90
Chapter 3.1.3 --- Human genome U133 Plus 2.0 array --- p.95
Chapter 3.1.4 --- Detection of changes in the proteome --- p.96
Chapter 3.1.5 --- MALDI-TOF MS --- p.97
Chapter 3.2 --- Materials --- p.99
Chapter 3.2.1 --- Solutions for cell culture use --- p.99
Chapter 3.2.2 --- Solutions for cloning --- p.99
Chapter 3.2.3 --- Buffers for cell cycle analysis --- p.99
Chapter 3.2.4 --- Buffers for two-dimensional (2D) electrophoresis --- p.100
Chapter 3.2.5 --- Solutions for silver staining --- p.101
Chapter 3.2.6 --- Solutions for Coomassie blue protein staining --- p.102
Chapter 3.2.7 --- Solutions for Western blotting --- p.102
Chapter 3.2.8 --- Solutions for mass spectrometry --- p.103
Chapter 3.3 --- Methods --- p.104
Chapter 3.3.1 --- Hypoosmotic stress --- p.104
Chapter 3.3.2 --- Heat shock --- p.104
Chapter 3.3.3 --- Oxidative stress treatment
Chapter 3.3.4 --- Chemical hypoxia --- p.104
Chapter 3.3.5 --- Treatment of forskolin --- p.106
Chapter 3.3.6 --- Culture of SHSY5Y cells and its differentiation --- p.106
Chapter 3.3.7 --- Cloning of pBUDCE4.1/ATQ --- p.106
Chapter 3.3.8 --- PCR product purification --- p.107
Chapter 3.3.9 --- Preparation of pEGFP.N1 vector for co-transfection --- p.109
Chapter 3.3.10 --- Transfection of HEK293 and HepG2 cells --- p.109
Chapter 3.3.11 --- Assays to characterize transient transfected HEK293 and HepG2 cells --- p.110
Chapter 3.3.11.1 --- Transfection efficiency monitoring --- p.110
Chapter 3.3.11.2 --- Cell cycle analysis --- p.112
Chapter 3.3.11.3 --- Cell doubling time measurement --- p.112
Chapter 3.3.11.4 --- Stress responsiveness --- p.113
Chapter 3.3.11.5 --- Oligonucleotide array analysis --- p.113
Chapter 3.3.11.5.1 --- Total RNA extraction --- p.113
Chapter 3.3.11.5.2 --- Oligonucleotide array preparations --- p.113
Chapter 3.3.11.5.3 --- Data analysis --- p.114
Chapter 3.3.11.6 --- Two-dimensional (2D) electrophoresis --- p.115
Chapter 3.3.11.6.1 --- Total protein extraction --- p.115
Chapter 3.3.11.6.2 --- Protein quantification --- p.115
Chapter 3.3.11.6.3 --- First dimension electrophoresis: isoelectric focusing (IEF) --- p.115
Chapter 3.3.11.6.4 --- Second dimension electrophoresis: SDS- --- p.116
Chapter 3.3.11.6.5 --- Silver staining --- p.116
Chapter 3.3.11.6.6 --- Spots detection --- p.117
Chapter 3.3.11.7 --- Preparations of samples for MALDI-TOF MS --- p.117
Chapter 3.3.11.7.1 --- Silver de-staining --- p.117
Chapter 3.3.11.7.2 --- In-gel tryptic digestion --- p.118
Chapter 3.3.11.7.3 --- Peptide extraction --- p.118
Chapter 3.3.11.7.4 --- ZipTip® samples desalting and concentrating --- p.119
Chapter 3.3.11.7.5 --- MALDI-TOF MS --- p.119
Chapter 3.3.11.8 --- Western blotting --- p.119
Chapter 3.3.11.8.1 --- Antibodies probing --- p.120
Chapter 3.3.11.8.2 --- Enhanced chemiluminescence's (ECL) assay --- p.121
Chapter 3.4 --- Results --- p.122
Chapter 3.4.1 --- Inducibility of antiquitin in HEK293 cells under xenobiotic stimulus --- p.122
Chapter 3.4.2 --- Inducibility of antiquitin in HEK293 and HepG2 cells under chemical hypoxia --- p.122
Chapter 3.4.3 --- Inducibility of antiquitin in HEK293 and HepG2 cells under hypoosmotic stress --- p.122
Chapter 3.4.4 --- Inducibility of antiquitin in HEK293 and HepG2 cells under heat shock --- p.122
Chapter 3.4.5 --- Inducibility of antiquitin in HEK293 and HepG2 cells under forskolin challenge --- p.128
Chapter 3.4.6 --- Expression of antiquitin in differentiating SHSY5Y cells by retinoic acid and N2 supplement --- p.128
Chapter 3.4.7 --- Overexpression of antiquitin in HEK293 and HepG2 cells --- p.128
Chapter 3.4.8 --- Viability of transfected HEK293 and HepG2 cells under hyperosmotic stress --- p.136
Chapter 3.4.9 --- Cell doubling times of transfected HEK293 and HepG2 cells --- p.143
Chapter 3.4.10 --- Cell cycle analysis of transfected HEK293 and HepG2 cells --- p.143
Chapter 3.4.11 --- "Western blot analysis of cyclin D, cyclin A and cyclin B of transfected HEK293 and HepG2 cells" --- p.148
Chapter 3.4.12 --- RNA quality control tests for oligonucleotide array analysis --- p.148
Chapter 3.4.13 --- Oligonucleotide array analysis on transfected HEK293 and HepG2 cells --- p.155
Chapter 3.4.14 --- Two-dimensional electrophoresis of transfected HEK293 and HepG2 cells --- p.169
Chapter 3.4.15 --- MALDI-TOF MS of transfected HEK293 and HepG2 cells --- p.169
Chapter 3.4.16 --- Genes and proteins upregulnted in the antiquitin transfected HEK293 and HepG2 cells --- p.190
Discussion --- p.197
Reference --- p.221
Appendix Materials used in the project --- p.243
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Cheng, Ching-Yang, and 鄭景陽. "Growth Dependence and Ultraviolet Light Inducibility of Rat Proliferating Cell Nuclear Antigen Gene Promotor." Thesis, 1997. http://ndltd.ncl.edu.tw/handle/30148786301492778196.

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Abstract:
碩士
國立清華大學
生命科學系
85
Proliferating Cell Nuclear Antigen (PCNA) can function as an auxiliary factor of DNA polymerase delta and epsilon, is required for DNA synthesis andDNA repair. After serum stimulation of quiescent cells, the level of PCNA mRNAincrease and reaches its peak before onset of DNA synthesis. In the present study, a rat PCNA promoter with chloramphicol acetyltransferase (CAT) as reportwas stably transfected into CHO.K1 cells. The promoter named d693 containingsequences between -693 and +125 in reference to the transcription initiation aite has been previously shown to be serum responsive in transient expression study. The CAT activity in the stable transfectants named d693-pCAT.K1 cells also display the same character of serum-responsiveness. In addition, UV irradiation on d693-pCAT.K1 cells induced rat PCNA promoter activity in dose-dependence manner. Such UV inducibility was seen with quiescent cells and growing cells. Furthermore, the promoter region of rat PCNA promoter that isresponsible for UV induction are delimited within the region from -70 to +125,which contains an AP-1 and ATF/CRE site and has been shown to be serum responsive and E1A oncoprotein responsive in the previous studies. Furthermore,the UV inducibility of PCNA promoter dose not require the activities of p53 and E2F, as both p53 and E2F inhibited PCNA promoter activity when they were overexpressed in d693-pCAT.K1 cells. Thus, UV inducibility of the rat PCNA promoter dose not require p53 and E2F.
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Chern, Jong-Tzer, and 沈仲澤. "The Development of Expression System Subject to Thermal Inducibility for High Productions of Recombinant Proteins." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/31398226012674491894.

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Abstract:
碩士
逢甲大學
化學工程學系
88
Expression vector appears to be a dispensable tool for genetic enginee-ring. The advance of DNA recombinant technology allows a versatile type of expression vectors to be developed by many research groups. However, many vectors developed to date are not completely suitable for engineering purpose. For instance, the leakiness of promoters and costly way of induc-tion are common problems, thereby leading to the limited use of these vectors for industrial application. Therefore, in this study we have attem-pted to develop three kinds of expression vectors with characteristic of tight regulation and economic way of induction. (1)The expression system based on the runaway plasmid containing T7 A1 promoter: To characterize this expression system, lacZ was fused to T7 A1 Pro- moter on a runaway plasmid. The strain harboring the recombinant plasmid was found to produce -galactosidase with 40,000 to 45,000 Miller uints upon thermal and chemical induction. In contrast, only 60 to 90 Miller units was produced at an uninduced state. Overall, it accounts for an induction ratio ranging from 350- to 570-fold, indicating the tightness of this expression system. (2)The expression system based on the runaway plasmid containing thermally regulated promoter: As shown above, the expression system based on runaway plasmid carrying a controllable promoter is particularly useful for tight regulation of cloned genes. However, the requirement for chemical induction calls for further improvement for this type of expression system. Accordingly, the expression system was further improved by incorporating the promoter subject to thermal control. As a consequence, the cell carrying the composite vector was capable of producing Pck with 13.15 mol/min/mg upon solely thermal induction. In sharp contrast, Pck yield was hardly detectable at an uninduced state. (3)The expression system based on the promoter under thermal regulation: Expression vectors derived from lac-type promoters are most commonly used. To make these systems more feasible use, lacI gene was mutated by site-directed mutagenesis to become thermally sensitive. Among four types of lacI gene thus mutated, the one with Ala241→Thr exhibited most thermal stability at low temperature (ranging 30 to 37℃), but it lost stability at high temperature (above 39℃). The vector carrying mutant lacI (Ala241→Thr) was constructed to overexpress lacZ, and the result showed that the recombinant strain yielded -galactosidase with 53, 000 Miller uints upon thermal induction. In sharp contrast, the uninduced level accounting for only 130 Miller units was generated. In conclusion, the expression systems developed in this study are characterized by stringent regulation. Moreover, the induction method by using temperature change for these vectors indicates an economic and easy way for controlling the expression of cloned genes in Escherichia coli. The successful demonstration of potential use of these vectors for high produ-ction of recombinant proteins has further suggested their promise in indu- strial application.
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Chang, Hsueh-Wei, and 張學偉. "The Regulation of Proliferating Cell Nuclear Antigen Gene Expression in UV Inducibility and Serum Responsiveness." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/53mu48.

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Abstract:
博士
國立清華大學
生命科學系
88
ABSTRACT Proliferating cell nuclear antigen (PCNA), also known as a processivity factor of DNA polymerase delta and epsilon, is required for eukaryotic cell DNA synthesis and repair. Expression of PCNA gene is growth-regulated and stress-responsive. This thesis is composed of four chapters. In chapter 1, the general introduction of PCNA is provided. Previously, the rat PCNA promoter responsive to UV irradiation and serum stimulation has been localized to nucleotides -70 and it contains an ATF (nucleotides -51 to -44) and AP-1 sites (nucleotides -64 to -58). In chapter 2, the rat PCNA promoter, and the endogenous PCNA mRNA and protein are demonstrated to be UV inducible in serum or serum-free recovery, suggesting that this UV inducibility is ubiquitous in cycling and non-cycling cells but not an artifact. Similarly, cells synchronized at all stages show that the UV inducibility of rat PCNA promoter is increased in dose-dependent manner. Hence, the PCNA gene is firstly reported to be UV inducible in all cell stages. Subsequently, the regulating mechanisms for UV inducibility of PCNA gene are addressed. Concerned with the DNA damage induced by UV irradiation, the DNA-dependent protein kinase (DNA-PK) mutant cells (xrs-6) were found to be UV inducible as well as its parental CHO.K1 cells. Moreover, the DNA-PK down-stream pathways are known to involve the p53 and DNA-PK can phosphorylate the p53. However, UV inducibility of rat and human PCNA expression is found to be independent on the UV induced p53 expression (chapter 2 and 3, respectively). These observations suggest that DNA damage checkpoint pathways is not essential for UV inducibility of PCNA gene. Therefore, the cell signal pathways except the DNA damage are investigated in the followings. Given the thiol-rich nature of PCNA and its role in proliferation, PCNA might be affected by redox change. N-acetyl-L-cysteine (NAC), a GSH precursor, was used and inhibited this UV inducibility of PCNA promoter, suggesting that the UV inducibility of PCNA involves the oxidative stress. For comparison to rat PCNA expression as shown in chapter 3, the role of p53 in human PCNA gene expression is studied by infection of the human papillomavirus type 16 E6 oncogene into human fibroblasts (HF). The introduction of E6 to human fibroblasts abrogates the function of cellular p53 and p21WAF1 and the cell growth control in this system. For serum stimulation, the confluent E6-infected cells (E6-HF) but not the parental cells exhibited a marked induction of PCNA. For UV exposure, both parental and E6-infected cells show the PCNA induction at a modest level in the recovery of serum or serum-free containing medium. These observations suggest that p53 may be involved in serum stimulation but not in UV induction of human PCNA gene expression. Therefore, the UV inducibility of both rat and human PCNA expression shows the same characteristic of p53 independence. To define the transcription factors responsive to UV inducibility and serum responsiveness of PCNA promoter, the gel mobility shift assays (EMSA) were used in chapter 4. In serum stimulated or UV irradiated cells, nuclear extracts incubated with probe containing ATF and AP-1 sites also show the increasing formation of the DNA:protein complexes. Similarly, the AP-1 DNA binding ability is enhanced by serum stimulation or UV irradiation. Consisting with the finding, serum responsiveness and UV inducibility of rat PCNA promoter were shown to be AP-1 related by CAT assay previously. This is firstly reported that the DNA:protein interactions require the simultaneous involvement of ATF/CRE and AP-1 sites as either element can abrogate the complexes in the competition experiment, suggesting there are cross talk between these two sites when cells were serum-stimulated or UV-irradiated. Moreover, both the distance and the sequence are essential to complex formation in serum-stimulated cells. ATF-1, but not ATF-2 and CREB, in association with other factors is involved in regulating the serum stimulation of the rat PCNA promoter activity via the proximal ATF and AP-1 sites. In conclusion, this thesis establishes the UV inducibility of rat and human PCNA gene and provides the mechanisms for understanding the serum stimulation and UV induction of PCNA response. The most important contribution is to demonstrate that the oxidative stress, but not the DNA damage checkpoint pathways including DNA-PK and p53, is one of the factors for regulating the UV inducibility of PCNA gene.
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Book chapters on the topic "Inducibility"

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Chen, Chang-Hwei. "Inducibility of Metabolizing Enzymes." In Activation and Detoxification Enzymes, 83–90. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4614-1049-2_9.

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Chen, Chang-Hwei. "Inducibility of Foreign Compound Metabolic Enzymes." In Xenobiotic Metabolic Enzymes: Bioactivation and Antioxidant Defense, 183–92. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-41679-9_16.

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Thomas, David J., Sheldon Morris, and P. C. Huang. "Age Dependence of Metallothionein Inducibility in Mouse Liver." In Trace Elements in Man and Animals 6, 295–96. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4613-0723-5_91.

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Pappas, P., V. Vasiliou, M. Karageorgou, and M. Marselos. "Changes in the Inducibility of a Hepatic Aldehyde Dehydrogenase." In Alcoholism, 115–20. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4684-5946-3_12.

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Kovács, Tibor. "Inducibility of the composition of frontier-to-root tree transformations." In Fundamentals of Computation Theory, 277–86. Berlin, Heidelberg: Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/3-540-51498-8_27.

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Kloeckener-Gruissem, Barbara, and Michael Freeling. "Relationship between Anaerobic Inducibility and Tissue-Specific Expression for the Maize Anaerobic Genes." In Plant Molecular Biology, 293–303. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4615-7598-6_27.

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Mano, H., S. Fujimaki, H. Hayashi, and M. Chino. "Wounding inducibility of axil gene, which induces auxin independent cell division of tobacco protoplast." In Plant Nutrition for Sustainable Food Production and Environment, 215–16. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-009-0047-9_55.

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Lobo, Juliet O., Roxanne L. Samrock, David W. Jayme, and Paul J. Price. "Sustained Inducibility of Cytochrome P450 Activity in Rat Hepatocytes Cultured in a Serum-Free Medium." In Animal Cell Technology: Basic & Applied Aspects, 195–201. Dordrecht: Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-2044-9_28.

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Inaba, M., S. Franceschelli, I. Suzuki, B. Szalontai, Y. Kanesaki, D. A. Los, H. Hayashi, and N. Murata. "Gene-Engineered Rigidification of Membrane Lipids Enhances the Cold Inducibility of Gene Expression in Synechocystis." In Advanced Research on Plant Lipids, 331–34. Dordrecht: Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-0159-4_77.

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Boobis, A. R., D. Sesardic, B. P. Murray, R. J. Edwards, and D. S. Davies. "Specificity and inducibility of cytochrome P-450 catalysing the activation of food-derived mutagenic heterocyclic amines." In N-Oxidation of Drugs, 345–55. Dordrecht: Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-011-3112-4_20.

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Conference papers on the topic "Inducibility"

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Potse, Mark. "Inducibility of Atrial Fibrillation Depends Chaotically on Ionic Model Parameters." In 2019 Computing in Cardiology Conference. Computing in Cardiology, 2019. http://dx.doi.org/10.22489/cinc.2019.410.

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ITAKURA, HIDETOSHI, YOSHIHISA ENJOJI, AKIYOSHI MORIYAMA, TAKESHI NAKAE, TAKAO SAKATA, MAHITO NORO, and KAORU SUGI. "THE RELATIONSHIP BETWEEN UNDERLYING HEART RHYTHM AND INDUCIBILITY OF VENTRICULAR FIBRILLATION IN BRUGADA SYNDROME." In Proceedings of the 31st International Congress on Electrocardiology. WORLD SCIENTIFIC, 2005. http://dx.doi.org/10.1142/9789812702234_0037.

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Bolmarcich, Jennifer, George R. Jackson, Mitchell Klausner, and Patrick J. Hayden. "Inducibility Of Xenobiotic Metabolizing Enzyme (XME) Activity In An In Vitro Human Airway (EpiAirway) Model." In American Thoracic Society 2010 International Conference, May 14-19, 2010 • New Orleans. American Thoracic Society, 2010. http://dx.doi.org/10.1164/ajrccm-conference.2010.181.1_meetingabstracts.a1757.

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Seemayer, CA, SA Kolb, J. Böni, M. Neidhart, B. Simmen, RE Gay, BA Michel, J. Schüpbach, and S. Gay. "AB0206 Investigation of the inducibility of retroviruses from rheumatoid arthritis synovial fibroblasts (ra-sf) and ra-synovial fluid cells (ra-sfc)." In Annual European Congress of Rheumatology, Annals of the rheumatic diseases ARD July 2001. BMJ Publishing Group Ltd and European League Against Rheumatism, 2001. http://dx.doi.org/10.1136/annrheumdis-2001.717.

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Reports on the topic "Inducibility"

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Sessa, Guido, and Gregory Martin. Role of GRAS Transcription Factors in Tomato Disease Resistance and Basal Defense. United States Department of Agriculture, 2005. http://dx.doi.org/10.32747/2005.7696520.bard.

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Abstract:
The research problem: Bacterial spot and bacterial speck diseases of tomato are causedby strains of Xanthomonas campestris pv. vesicatoria (Xcv) and Pseudomonas syringae pv.tomato (Pst), respectively. These bacteria colonize aerial parts of the plant and causesignificant losses in tomato production worldwide. Protection against Xcv and Pst bycultural practices or chemical control has been unsuccessful and there are only limitedsources of genetic resistance to these pathogens. In previous research supported in part byBARD IS-3237-01, we extensively characterized changes in tomato gene expression uponthe onset of spot and speck disease resistance. A remarkable finding of these studies wasthe inducibility in tomato leaves by both Xcv and Pst strains of genes encodingtranscriptional activator of the GRAS family, which has not been previously linked todisease resistance. Goals: Central goals of this research were to investigate the role of GRAS genes in tomatoinnate immunity and to assess their potential use for disease control.Specific objectives were to: 1. Identify GRAS genes that are induced in tomato during thedefense response and analyze their role in disease resistance by loss-of-function experiments.2. Overexpress GRAS genes in tomato and characterize plants for possible broad-spectrumresistance. 3. Identify genes whose transcription is regulated by GRAS family. Our main achievements during this research program are in three major areas:1. Identification of tomato GRAS family members induced in defense responses andanalysis of their role in disease resistance. Genes encoding tomato GRAS family memberswere retrieved from databases and analyzed for their inducibility by Pst avirulent bacteria.Real-time RT-PCR analysis revealed that six SlGRAS transcripts are induced during theonset of disease resistance to Pst. Further expression analysis of two selected GRAS genesshowed that they accumulate in tomato plants in response to different avirulent bacteria orto the fungal elicitor EIX. In addition, eight SlGRAS genes, including the Pst-induciblefamily members, were induced by mechanical stress in part in a jasmonic acid-dependentmanner. Remarkably, SlGRAS6 gene was found to be required for tomato resistance to Pstin virus-induced gene silencing (VIGS) experiments.2. Molecular analysis of pathogen-induced GRAS transcriptional activators. In aheterologous yeast system, Pst-inducible GRAS genes were shown to have the ability toactivate transcription in agreement with their putative function of transcription factors. Inaddition, deletion analysis demonstrated that short sequences at the amino-terminus ofSlGRAS2, SlGRAS4 and SlGRAS6 are sufficient for transcriptional activation. Finally,defense-related SlGRAS proteins were found to localize to the cell nucleus. 3. Disease resistance and expression profiles of transgenic plants overexpressing SlGRASgenes. Transgenic plants overexpressing SlGRAS3 or SlGRAS6 were generated. Diseasesusceptibility tests revealed that these plants are not more resistant to Pst than wild-typeplants. Gene expression profiles of the overexpressing plants identified putative direct orindirect target genes regulated by SlGRAS3 and SlGRAS6. Scientific and agricultural significance: Our research activities established a novel linkbetween the GRAS family of transcription factors, plant disease resistance and mechanicalstress response. SlGRAS6 was found to be required for disease resistance to Pstsuggesting that this and possibly other GRAS family members are involved in thetranscriptional reprogramming that takes place during the onset of disease resistance.Their nuclear localization and transcriptional activation ability support their proposed roleas transcription factors or co-activators. However, the potential of utilizing GRAS familymembers for the improvement of plant disease resistance in agriculture has yet to bedemonstrated.
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