Dissertations / Theses on the topic 'Incompatibility system'

[EN] Self-incompatibility (SI) comprises a compendium of molecular intraspecific barriers, controlled by the S-locus, which enhances outcrossing and prevents inbreeding. Solanaceae, Plantaginaceae and Rosaceae exhibit the Gametophytic SI (GSI) type where specific recognition is controlled by S-RNases and S-locus F-box (SFB) proteins as the female and male S-determinants, respectively. On the other hand, unlinked S-locus genes known as modifier factors are also completely necessary for the mechanism to function. The GSI system seems to be basically preserved in Prunus but striking differences with Solanaceae and other Rosaceae have also been observed. On the basis of this background, this thesis is focused on the identification and characterization of modifiers involved in Prunus GSI to improve our understanding of the underlying mechanism. Previous works in apricot showed that an S-locus unlinked mutation expressed in pollen and located at the distal end of chr. 3 (M-locus) confers self-compatibility in the cv. 'Canino'. In this work, another self-compatible apricot cultivar, named 'Katy', was molecular and genetically analyzed. Similarly, an S-locus unlinked pollen-part mutation was found to cause the loss of self-incompatible response. A mapping strategy based on segregation distorted loci mapped 'Katy' mutation (referred as m-mutation) at the distal end of chr. 3, in a region overlapping with that identified for 'Canino' M-locus. A new screening was carried out to identify additional self-compatible mutants in apricot cultivar/accessions from germplasm banks. Through S-genotyping, three uncategorized S-alleles were recovered and two new mutations putatively conferring self-compatibility (SC) by affecting the male S-determinant SFB were detected. Additionally, M-genotyping showed that the same mutated m-haplotype was shared by 'Canino' and 'Katy', but also by 17 cultivars more from North-America and Western-Europe. A widely distributed haplotype M1-0 was proposed as the putative m-haplotype ancestor suggesting that it arose much later in time than SC-allele, a mutation in the S-locus also conferring SC in apricot. In order to identify this mutation, an integrative genetic, genomic and transcriptomic approach based on NGS data from 'Canino', 'Katy' and the self-incompatible apricot cultivar 'Goldrich' was carried out. This approach led to identify a unique polymorphism able to explain the self-compatible phenotype, a FaSt insertion type of 358-bp in coupling with the m-haplotype within a gene encoding a disulfide bond A-like oxidoreductase (named PaMDOr). PaMDOr was found to be differentially over expressed in mature anthers and the FaSt insertion is predicted to produce a truncated protein. These two findings also support PaMDOr as the pollen-part mutated modifier conferring SC in apricot. Furthermore, phylogenetic analyses suggest PaMDOr as a putative paralog of its contiguous gene (PaM-8), that emerged after the split of the Rosaceae and Solanaceae and which function became essential for the proper functioning of the GSI system in Prunus. Aimed to shed light on the differences and similarities between the S-RNase-based GSI systems in Rosaceae and Solanaceae, orthology relationships were analyzed for modifiers. Putative orthologs were found for NaTrxh, SBP1 and MdABCF in Prunus but a more complex evolutionary pattern was detected for 120K, NaStEP and NaPCCP. Thus, in spite of the differences, it can be hypothesized that part of the GSI modifier factors are shared by both families. As a whole, the multidisciplinary strategy developed in this thesis has allowed us to identify a novel modifier factor (PaMDOr) essential for the self-incompatible response in Prunus as the most significant contribution. In addition, new sources of SC have been detected in apricot and the orthology analysis helped to deepen our understanding on evolutionary aspects of the S-RNase-based GSI system exhibited by Prunus.
[ES] La autocompatibilidad (AI) comprende un conjunto de barreras moleculares intraespecíficas, controladas por el locus S, que favorecen la polinización cruzada y previenen de la endogamia. Solanáceas, Plantagináceas y Rosáceas presentan la llamada AI gametofítica (AIG) donde el reconocimiento específico está controlado por ARNasas-S y proteínas F-box del locus S (SFB) como los determinantes femenino y masculino, respectivamente. Por otra parte, genes no ligados al locus S, conocidos como factores modificadores, son también totalmente necesarios para la correcta regulación del mecanismo. El sistema AIG parece estar básicamente conservado en Prunus pero se han observado notables diferencias con Solanáceas y otras Rosáceas. Con estos antecedentes, el trabajo realizado en esta tesis se ha centrado en la identificación y caracterización de factores modificadores de la AIG en Prunus con el fin de mejorar nuestro conocimiento del mecanismo subyacente. Trabajos previos en albaricoquero mostraron la existencia de una mutación expresada en el polen y no ligada al locus S, que se localiza en el extremo distal del cr.3 (locus M) y que es capaz de conferir autocompatibilidad (AC) en el cultivar 'Canino'. En esta tesis, otro cultivar de albaricoquero autocompatible llamado 'Katy' fue genética y molecularmente analizado. De manera parecida a 'Canino', una mutación que afectaba a un factor no ligado al locus S expresado en el polen era el causante de la pérdida de la respuesta autoincompatible. La mutación en 'Katy' se consiguió mapear en el extremo distal del cr.3 (mutación m), una región que solapa con la identificada para 'Canino'. Una búsqueda para la identificación de nuevo mutantes autocompatibles en cultivares/accesiones de albaricoquero procedentes de bancos de germoplasma fue realizado. Por medio del genotipado del locus S, 3 alelos S no clasificados con anterioridad fueron hallados, mientras que 2 nuevas mutaciones autocompatibles que parecen haber afectado al determinante S masculino SFB fueron detectadas. Adicionalmente, el genotipado para el locus M mostró que el mismo haplotipo m mutado está compartido por 'Canino' y 'Katy' y 17 cultivares más del norte de América y el oeste de Europa. El haplotipo M1-0 ha sido propuesto como posible ancestro del haplotipo m, sugiriendo que éste surgió mucho más tarde que el alelo Sc, mutación del locus S que también confiere AC en albaricoquero. Con el objetivo de identificar esta mutación, un abordaje integral tanto a nivel genético como genómico y transcriptómico mediante datos NGS procedentes de 'Canino', 'Katy' y del cultivar de albaricoquero autoincompatible 'Goldrich' fue llevado a cabo. Esta aproximación sirvió para identificar un único polimorfismo capaz de explicar el fenotipo de AC, una inserción tipo FaSt de 358 pb en acoplamiento con el haplotipo m en un gen que codifica para una disulfide bond A-like oxidoreductase (PaMDOr). PaMDOr mostró estar diferencialmente sobre-expresado en anteras maduras, mientras que la inserción FaSt predice la formación de una proteína truncada. Estos dos hechos apoyan a PaMDOr como el factor modificador de la parte del polen que confiere AC en albaricoquero. Adicionalmente, análisis filogenéticos sugieren que PaMDOr podría ser un parálogo del gen contiguo (PaM-8) que surgió después de la división de Rosáceas y Solanáceas, cuya función ha llegado a ser esencial para el correcto funcionamiento del sistema en Prunus. A fin de arrojar cierta luz en las diferencias y similitudes entre los sistemas de AIG basado en ARNasas-S de Rosáceas y Solanáceas, las relaciones de ortología para factores modificadores fueron estudiadas. Ortólogos candidatos fueron encontrados para NaTrxh, SBP1 y MdABCF, sin embargo, un patrón evolutivo más complejo fue observado para NaStEP, 120K y NaPCCP. De modo que, a pesar de las diferencias, se puede hipotetizar que una parte de los modificadores de la AIG están comparti
[CAT] L'autocompatibilitat (AI) comprèn un conjunt de barreres moleculars intraespecífiques, controlades pel locus S, que afavorixen la pol·linització creuada i prevé de l'endogàmia. Solanàcies, Plantaginàcies i Rosàcies presenten l'anomenada AI gametofítica (AIG) on el reconeixement específic està controlat per ARNases-S i proteïnes F-box del locus S (SFB) com a determinants femení i masculí, respectivament. Per un altra banda, gens no lligats al locus S, coneguts com factors modificadors, són també totalment necessaris per a la correcta regulació del mecanisme. El sistema AIG pareix estar bàsicament conservat en Prunus, però s'han observat notables diferències amb Solanàcies i altres Rosàcies. Amb estos antecedents, el treball realitzat durant aquesta tesi se ha focalitzat en la identificació i caracterització de factors modificadors de l'AIG en Prunus a fi d millorar el nostre enteniment del mecanisme subjacent. Treballs previs a l'albercoquer mostraren l'existència d'una mutació expressada al pol·len no lligada al locus S, la qual està localitzada a l'extrem distal del cr.3 (locus M) i es capaç de conferir autocompatibilitat (AC) al cultivar 'Canino'. En aquest treball, un altre cultivar d'albercoquer autocompatible anomenat 'Katy' va ser genètica i molecularment analitzat. De manera pareguda a 'Canino', una mutació que afecta a un factor no lligat al locus S expressat al pol·len era la causa de la perduda de la resposta autoincompatible. La mutació a 'Katy' es va mapetjar a l'extrem distal del cr.3 (mutació m) en una regió solapant amb la identificada per a 'Canino'. Una recerca per a la identificació de nous mutants autocompatibles en cultivars i/o accessions d'albercoquer procedents de bancs de germoplasma va ser portada a terme. Mitjançant el genotipatge del locus S, 3 al·lels S no classificats amb anterioritat van ser trobats, mestres que dos noves mutacions AC que pareixen haver afectat al determinant S masculí SFB varen ser detectades. Amés, el genotipatge del locus M va mostrar que el mateix haplotip m mutat està compartit per 'Canino' i 'Katy', però també per 17 cultivars més del nord d'Amèrica i l'oest d'Europa. El haplotip M1-0, ampliamente distribuït, ha sigut proposat com a possible ancestre del haplotip m, sugerint que aquest va sorgir més tard que el al·lel Sc, una mutació al locus S que també conferix AC a l'albercoquer. Amb l'objectiu d'identificar aquesta mutació, un abordatge integral tant a nivell genètic com genòmic i transcriptòmic mitjançant diversos tipus de dades NGS provinents de 'Canino', 'Katy' i del cultivar d'albercoquer autoincompatible 'Goldrich' va ser portat terme. Aquesta aproximació va permetre identificar un únic polimorfisme capaç d'explicar el fenotip AC, es tracta d'una inserció de 358 pb en adaptament amb el haplotip m en un gen que codifica per a disulfide bond A-like oxidoreductase (PaMDOr). PaMDOr va mostrar estar diferencialment sobre-expressat en anteres madures, mentres que la inserció FaSt prediu la formació d'una proteïna truncada. Estos dos fets recolzen a PaMDOr com al factor modificador de la part del pol·len que conferix AC en albercoquer. A més a més, anàlisis filogenètics suggerixen que PaMDOr podria ser un paràlog del seu gen contigu (anomenat PaM-8) que va sorgir després de la divisió de Rosàcies i Solanàcies, en la qual la funció ha arribat a ser fonamental per al correcte funcionament del sistema d'AIG a Prunus. A fi de tirar certa llum en quant a les diferències i similituds entre els sistemes d'AIG basats en ARNases-S de Rosàcies i Solanàcies, les relacions d'ortologia per als factors modificadors va ser estudiat. Ortòlogs candidat van ser trobats per a NaTrxh, SBP1 i MdABCF, no obstant, un patró evolutiu més complex va ser observat per a NaSTeP, 120K i NaPCCP. De tal manera que, a pesar de les diferències, es pot plantejar la hipòtesi de que una part dels modificadors de l'AIG estan
Muñoz Sanz, JV. (2016). Crossability barriers in Prunus: the role of modifiers in the regulation of the gametophytic self-incompatibility system [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/68489
TESIS

Dans un contexte de succès de colonisation des espèces invasives, la compréhension d’un front d’invasion est essentielle afin d’identifier des leviers de gestion. En France, Ludwigia grandiflora subsp hexapetala a envahi la plupart de réseaux hydrographiques avec des populations fertiles et stériles. Les objectifs de cette thèse étaient l’identification des facteurs influençant sa reproduction sexuée et la caractérisation de sa diversité génétique afin d’inférer le mode de reproduction efficace et de comprendre l’histoire du front d’invasion. La biologie florale et la fructification des populations fertiles et stériles dans différentes conditions climatiques et après différents croisements contrôlés ainsi que la diversité génétique des populations via un set de marqueurs moléculaires SNP ont été évaluées,Ces travaux ont montré que la reproduction sexuée de Ludwigia grandiflora subsp hexapetala est sous contrôle d’un système d’auto-incompatibilité hétéromorphique « à action tardive » de type distyle-homostyle. Nous avons identifié deux fronts d’invasion avec deux points de rencontre. Le système de reproduction efficace majoritaire est la clonalité avec un signal d’allogamie au niveau des 2 « hot-spot » de diversité, suggérant une priorisation de gestion à leurs niveaux. Ces études pourront être étendues à d’autres fronts d’invasion en Europe et aux USA
In a context of successful colonization of invasive species, understanding of an invasion front is essential in order to identify management levers. In France, Ludwigia grandiflora subsp hexapetala has invaded most river systems with fertile and sterile populations. The objectives of this thesis were the identification of factors influencing its sexual reproduction and the characterisation of its genetic diversity in order to infer the efficient mode of reproduction and to understand the history of the invasion front.Floral biology and fructification of fertile and sterile populations in different climatic conditions and after different controlled crosses as well as the genetic diversity of the populations via a set of SNP molecular markers were evaluated. These studied showed that the sexual reproduction of Ludwigia grandiflora subsp hexapetala is under the control of a "late-acting" heteromorphic self-incompatibility system of the distyle-homostyle type. We have identified two invasion fronts with two meeting points. The majority efficient breeding system is clonality with an allogamy signal at the level of the 2 diversity hot spots, suggesting management prioritization at their levels. These studies could be extended to other invasion fronts in Europe and the USA

Mestrado em Biologia Molecular e Celular
To understand the molecular basis of the S-RNase-based gametophytic selfincompability system of subtribe Pyrinae from Rosaceae family, in this work, Malus x domestica species was used to determine the genes involved in pollen S-specificity. Previously 18 F-box genes, similar to the S-locus F-box brothers (SFBBs), were identified by conventional polymerase chain reaction techniques and with the pollen transcriptome analysis of nine M. domestica cultivars. However, not all 10 S-haplotypes, covered by the nine M. domestica cultivars used, have been characterized for all SFBB genes, and 12 SFBB sequences found, align as highly divergent sequences in 12 SFBB genes. Thus, in this work, by cloning and sequencing analysis of two highly diverged alleles, namely, SFBBGu8 and SFBBN3 of SFBB5 and SFBB1 genes, respectively, was concluded that SFBBGu8 sequence represent the S1- and S24- diverged alleles of SFBB5 gene, while SFBBN3 sequence represent the S28- diverged allele of SFBB1 gene. Additionally, for SFBB5 gene there is no SFBB copy number variation. This pattern was also observed for other 12 SFBB genes. For 15 of the 18 SFBB genes identified, linkage with the S-RNase gene (gene involved in pistil S-specificity) was established by segregation analysis of the F1 progeny from the cross of Fuji (S1, S9) with Honeycrisp (S2, S24), previously genotyped. As result, for SFBB2, SFBB3, SFBB4, SFBB6, SFBB7, SFBB8, SFBB9, SFBB10, SFBB11, SFBB12, SFBB13, SFBB14 and SFBB16 genes, linkage with at least one S-RNase allele was established. Thus, since these 13 SFBB genes also present pollen-specific expression and S-haplotypespecific polymorphism, these genes are pollen S-genes. In conclusion, consistent features with the collaborative non-self recognition model, were identified in M. domestica species, such as, large number of SFBB genes and the presence of highly diverged SFBB alleles, that may be conserved in other S-haplotypes, and thus, involved in the recognition of a particular non-self S-RNase. However, in M. domestica species, it was not verified SFBB copy number variation within the different S-haplotypes, as observed in Petunia. The characterization of the S-pollen genes involved in the self-incompatibility mechanism in M. domestica species is the first step to characterize self-pollen rejection mechanism in Pyrinae subtribe.
Para compreender a base molecular do sistema de auto-incompatibilidade gametofítica baseada em S-RNases da subtribo Pyrinae da família Rosaceae, neste trabalho, a espécie Malus x domestica foi utilizada para determinar os genes envolvidos na especificidade S do pólen. Previamente 18 genes F-box, semelhantes aos “S-locus F-box brothers” (SFBBs), foram identificados por uma abordagem baseada em técnicas convencionais de reação em cadeia da polimerase e por análise de transcriptomas do pólen de nove cultivares de M. domestica. Contudo, nem todos os 10 haplótipos S, cobertos pelos nove cultivares de M. domestica utilizados, foram caracterizados para todos os genes SFBB, e 12 sequências SFBB encontradas, alinham como sequências altamente divergentes em 12 genes SFBB. Assim, neste trabalho, por clonagem e sequenciação de dois alelos altamente divergentes, nomeadamente, SFBBGu8 e SFBBN3 dos genes SFBB5 e SFBB1, respectivamente, concluiu-se que a sequência SFBBGu8 representa os alelos divergentes S1- e S24- do gene SFBB5, enquanto que a sequência SFBBN3 representa o alelo divergente S28- do gene SFBB1. Adicionalmente, para o gene SFBB5 não existe variação do número de cópias dos SFBBs. Este padrão foi também observado para outros 12 genes SFBB. Para 15 dos 18 genes SFBB identificados, foi determinada associação com o gene da SRNase (gene envolvido na especificidade S do pistilo) através de análises de segregação da progenia F1 resultante do cruzamento de Fuji (S1, S9) com Honeycrisp (S2, S24), previamente genotipada. Como resultado, para os genes SFBB2, SFBB3, SFBB4, SFBB6, SFBB7, SFBB8, SFBB9, SFBB10, SFBB11, SFBB12, SFBB13, SFBB14 e SFBB16, foi estabelecida associação com pelo menos um alelo da S-RNase. Assim, dado que estes 13 genes SFBB também apresentam expressão exclusiva no pólen e polimorfismo específico para cada haplótipo S, estes genes são genes S do pólen. Em conclusão, foram identificadas características consistentes com o modelo “collaborative non-self-recognition” em M. domestica, tais como, um grande número de genes SFBB e a presença de alelos SFBB altamente divergentes, que podem ser conservados noutros haplótipos S e assim, estar envolvidos no reconhecimento de uma S-RNase não-própria. Contudo, em M. domestica, não ser verificou variação do número de cópias dos SFBBs nos diferentes haplótipos S, como observado em Petunia. A caraterização dos genes S do pólen envolvidos no mecanismo de auto-incompatibilidade em M. domestica é o primeiro passo para caracterizar o mecanismo de rejeição do pólen do próprio, na subtribo Pyrinae.

Antibody-mediated rejection is central to ABO incompatible transplantation as well as to xenotransplantation. The xenoantigen alpha-Gal has a highly analogous carbohydrate structure to the human blood group antigens, and both require memory B cell activation for antibody production. We hypothesize that B cells, reactive to the alpha-Gal xenoantigen and B blood group antigen, require the presence of fully activated T cells in order to survive and proliferate in vitro, contrary to the traditional theory that humoral response to carbohydrate antigens is a T cell-independent process. When we compared the capacity of B cells to proliferate, we observed that activated T cells were necessary for B cell proliferation even in the presence of carbohydrate-derived antigens. A relevant question was also to investigate the role of a specific class of T cells: the CD1d-restricted iNKT cells, in the activation of alpha-Gal and B blood group-reactive B cells. The iNKT cells have the specificity of being reactive to glycolipids and are capable of producing both T helper 1 and T helper 2 cytokine responses. We therefore wanted to determine the role of the iNKT cells as mediators of a T helper 2-type response when B cells were exposed to a glycolipid antigen expressing the alpha-Gal epitope or the human B blood group antigen. We observed that, if the interaction between B cells and iNKT cells is blocked, neither B cell proliferation nor antibody production occurs. These results suggest therefore the importance of the iNKT cell category of T helper cells in the response to alpha-Gal and ABO-blood group glycolipids.

La résistance mécanique des bétons exigée actuellement impose une diminution du rapport eau/ciment et l’utilisation de superplastifiant pour faciliter la mise en œuvre du matériau frais. Les produits de type polycarboxylate sont très efficaces à court terme, mais peuvent mener à une perte rapide de l’ouvrabilité en cas d’incompatibilité liant/superplastifiant. L’objet de ce travail est l’identification des phases cimentaires impliquées dans ces variations rhéologiques. Dans un premier temps, l’écoulement de pâtes superplastifiées est évalué par un rhéomètre muni d’un système de mesure à boule. Les combinaisons compatibles s’écoulent selon le modèle de Bingham. Une incompatibilité entraîne soit une augmentation du seuil d'écoulement et de la viscosité plastique, soit une évolution des propriétés rhéologiques en un fluide d’Herschel-Bulkley. Dans un deuxième temps, des combinaisons ciment/superplastifiant sont caractérisées par des essais rhéologiques. L’évaluation du raidissement de pâtes et la caractérisation chimique des ciments hydratés montrent que la consistance est liée à la quantité et à la microstructure de l’ettringite formée. Ces paramètres dépendent de la phase aluminate et des sulfates de calcium initialement présents. Enfin, l’étude de systèmes cimentaires de synthèse établit que la solubilité des sulfates de calcium en présence de superplastifiant est augmentée du fait, vraisemblablement, de la complexation des ions Ca2+ par les polycarboxylates. La variété orthorhombique de la phase aluminate plus réactive que la variété cubique, mène, quant à elle, à la formation d’une quantité importante d’ettringite en forme d’aiguilles
Currently required concrete strength imposes a lower water/cement ratio and use of superplasticizer to make easier the fresh material implementation. Polycarboxylate type products are very effective in the short term but can lead to a fast loss of workability in case of binder/superplasticizer incompatibility. The object of this work is the identification of cementitous phases involved in these rheological variations. Initially, flow of various superplasticized pastes is evaluated by a rheometer fitted with a ball measuring system. Compatible combinations flow like a Bingham’s fluid. An incompatibility results in an increase of yield stress and plastic viscosity or rheological properties evolution into Herschel-Bulkley’s fluid. Then, cement/superplasticizer combinations are characterized by rheological tests. Stiffening assessment of pastes and chemical characterisation of hydrated cements show that consistency is associated with amount and microstructure of formed ettringite. These parameters depend on initially present aluminate phase and calcium sulphates. Finally, study of synthetic cementitous systems states that calcium sulphates solubility, in presence of superplasticizer, is increased probably because Ca2+ ions complexation by polycarboxylates. As for the orthorhombic variety of aluminate phase, more reactive than cubic variety, it leads to a large amount of needle-shaped ettringite

The following article aims to give an overview about the compatibility between the social security benefits provided by the State, which are free and are not conditional to the development of an activity, with the salary, which has its Foundation, inescapably, in the development of an activity, either of labour, civil service or professional nature. To do this, the author will attempt to make a transition between the radical incompatibility that seems to exist between the two concepts towards a possible compatibility through certain mechanisms of adjustment.
El presente artículo pretende brindar una visión sobre la compatibilidad que existe entre las prestaciones de seguridad social que brinda el Estado, que son gratuitas y no se encuentran condicionadas al desarrollo de una actividad, con el salario, el cual tiene su fundamento, ineludiblemente, en el desarrollo de una actividad ya sea de naturaleza laboral, funcionarial o profesional. Para ello, el autor se intentará realizar una transición entre la incompatibilidad radical que parece existir entre ambos conceptos hacia una posible compatibilidad a través de ciertos mecanismos de ajuste.

Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2015-03-31T13:42:09Z No. of bitstreams: 2 Dissertação - Elga de Fátima Almeida - 2014.pdf: 1529680 bytes, checksum: ee90611c2799a2036aadcc8089f3bef6 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)
Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2015-04-01T10:46:20Z (GMT) No. of bitstreams: 2 Dissertação - Elga de Fátima Almeida - 2014.pdf: 1529680 bytes, checksum: ee90611c2799a2036aadcc8089f3bef6 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)
Made available in DSpace on 2015-04-01T10:46:20Z (GMT). No. of bitstreams: 2 Dissertação - Elga de Fátima Almeida - 2014.pdf: 1529680 bytes, checksum: ee90611c2799a2036aadcc8089f3bef6 (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5) Previous issue date: 2014-04-23
Fundação de Amparo à Pesquisa do Estado de Goiás - FAPEG
The plant reproductive biology involves all the features (morphological, anatomical or physiological) of a species that participate in the reproduction of its individuals. The Fabaceae family is one of the most important of the Cerrado, the genus Bauhinia being one of the most diversified. Several studies with species of Bauhinia L. showed diversity in floral shapes, reproductive phenology, reproductive system and interactions with different groups of pollinators. In general, the Bauhinia species are outcrossing and have low reproductive efficiency. Bauhinia brevipes is common in the municipality of Goiás-GO and has floral features compatible with the syndrome chiropterophily: white flowers that release a strong odour overnight, producing large amounts of nectar with low sugar concentration. Flowers of B. brevipes may have reduced ovaries or anthers with low production of viable pollen, indicating that this species moves towards sexual separation in some flowers. Although the visit the bats to flowers were not seen, their flights over the plants were observed. A test of exclusion of diurnal pollinators showed the efficiency of nocturnal pollinators in B. brevipes. The flowers of B. brevipes not have herkogamy as the main factor to avoid inbreeding, but some flowers positioned above the anthers stigma were observed. The species present gametophytic selfincompatibility and therefore requires the pollinators visit to ensure pollinating pollen exchange among different and unrelated plants. Bauhinia brevipes has an average 20% of reproductive efficiency. In general, the species showed a high level of pollen limitation (0.73). The largest fruit production occurs in the middle position of the inflorescence. Most of fruit produced occurs in the median position of the inflorescence, when possibly the availability of pollen in the system is higher and resources are available for their development.
A biologia reprodutiva de plantas trata de diversos aspectos relacionados a mecanismos como o de polinização (e.g., morfologia e biologia floral, interação plantapolinizador), do sistema de reprodução (sistema sexual, de incompatibilidade, modos de reprodução – sexuada e assexuado-vegetativa), eficiência reprodutiva, dentre outros. A família Fabaceae é uma das mais importantes do Cerrado, sendo o gênero Bauhinia um dos mais diversificados. Diversos estudos realizados com espécies de Bauhinia L. mostraram a diversidade na fenologia, formas florais, sistema reprodutivo e interações com diferentes grupos de polinizadores. Embora aconteça autopolinização em algumas espécies do gênero, a xenogamia é a forma mais frequente de reprodução, e espécies autoincompatíveis são muito comuns. Bauhinia brevipes é comum no Cerrado da região do Município de Goiás-GO e possui características florais compatíveis com a síndrome da quiropterofilia: flores brancas que liberam odor forte durante a noite, produção de grande volume de néctar com baixa concentração de açúcar. Flores de B. brevipes podem apresentar ovário reduzido ou anteras com baixa produção de pólen viável, indicando que esta espécie caminha para separação sexual em algumas flores. Apesar de não serem visualizadas as visita dos morcegos às flores, que foram observados em sobrevoo na população, um teste de exclusão de polinizadores diurnos mostrou a eficiência dos polinizadores noturnos. As flores de B. brevipes não possuem hercogamia como principal fator para evitar a endogamia, porém existe na população uma porcentagem de flores com estigma posicionado acima do conjunto das anteras. A espécie apresenta autoincompatibilidade gametofítica e, portanto, necessita da visita de polinizadores para garantir a troca de pólen entre plantas diferentes e não aparentadas. Bauhinia brevipes apresenta uma eficiência reprodutiva em média de 20% das flores produzidas. De forma geral, a espécie apresentou um elevado índice de limitação polínica (0,73). A maior produção de fruto ocorre na posição mediana da inflorescência. As flores abertas nesta posição estão expostas, possivelmente, a uma alta disponibilidade de pólen no sistema, e os recursos maternos estão disponíveis em maior quantidade para o desenvolvimento de seus frutos.

Made available in DSpace on 2016-04-26T20:22:58Z (GMT). No. of bitstreams: 1 Luciana de Oliveira Bueno.pdf: 692348 bytes, checksum: f81580072edc0fe11acf77b5b159438d (MD5) Previous issue date: 2014-08-01
The International Criminal Court represented a major breakthrough in the international scenery, as the accountability of agents who commit extremely serious crimes because it was created in a permanent and complementary manner to national jurisdictions, respecting the principles of universality, complementarity, natural justice and independence. This Court, which differed from background experiences, for their essential characteristics, was structured in an organized way, giving the prosecutor the important investigative role, which greatly contributed to the achievement of the Court work. The Court's decisions, in turn, are uttered with the observance of the principles of legal reserve and prior criminal law, giving them greater legal certainty and fairness. However, what is observed is that the States that are committed to cooperate with the work of the Court, often do not contribute to the investigation or to implement the decisions of the Court, which has sparked many debates about the need to apply the law of force rather than the force of law. Another issue of great importance lies in the compatibility of the rules laid down in the Rome Statute with the Brazilian Federal Constitution, which, in some cases, apparently contrasts with our legal system. However, as it will be seen throughout this study, the problem of effectiveness slips in the very development of the countries involved in the conflict and the alleged incompatibilities exist in appearance only and do not constitute in reality
O Tribunal Penal Internacional representou um grande avanço no cenário internacional, quanto à responsabilização de agentes que cometem crimes de extrema gravidade, pois foi criado de forma permanente e complementar às jurisdições nacionais, respeitando os princípios da universalidade, complementaridade, juiz natural e independência. Esse Tribunal, que se diferenciou das experiências antecedentes, por suas características essenciais, foi estruturado de forma organizada, atribuindo ao Procurador o importante papel investigativo, o que muito contribuiu para a concretização dos trabalhos da Corte. As decisões do Tribunal, por sua vez, são proferidas com a observância dos princípios da reserva legal e da anterioridade da lei penal, conferindo-lhes maior segurança jurídica e imparcialidade. Entretanto, o que se observa é que os Estados, que assumem o compromisso de cooperar com os trabalhos da Corte, por muitas vezes não contribuem com a investigação ou para a execução das decisões proferidas pelo Tribunal, o que tem suscitado diversos debates acerca da necessidade da aplicação do direito da força em detrimento da força do direito. Outra questão de grande relevo reside na compatibilidade das normas previstas no Estatuto de Roma com a Constituição Federal brasileira, as quais, em algumas hipóteses, aparentemente contrastam com nosso ordenamento jurídico. Contudo, como se verá no decorrer desse estudo, o problema da efetividade resvala no próprio desenvolvimento dos países envolvidos nos conflitos e as supostas incompatibilidades só existem na aparência e não se configuram na realidade

Depuis les années 1950, l'intensification des pratiques agricoles concourt à une augmentation de la pression anthropique entraînant une raréfaction des espèces spécialistes des parcelles cultivées, ce qui contribue à l'érosion de la diversité biologique des agroécosystèmes. Parmi les espèces en déclin, on compte de nombreuses messicoles dont le bleuet (Centaurea cyanus L.), fleur emblématique des moissons. Or, cette espèce peut rendre des services écosystémiques comme hôte de prédateurs de ravageurs des cultures et ressource privilégiée de certains pollinisateurs qui justifieraient son maintien dans les champs. Nous étudions ici des facteurs biologiques qui pourraient potentiellement contribuer à son déclin : distribution spatiale, potentialité de croissance, diversité génétique des populations, survie des semences, système de reproduction. Nous avons montré, par deux campagnes de relevés, que C. cyanus n'est pas une espèce indicatrice de diversité floristique dans la parcelle cultivée. Toutefois, lorsque que le bleuet est rare dans une région, il est associé à d'autres messicoles peu fréquentes. Par contre, s'il est commun, il est associé aux zones ayant la plus forte diversité végétale. Une expérience de semis dans différents compartiments de l'agrosystème, en absence de traitements herbicides, a mis en évidence que la potentialité de croissance des bleuets est plus élevée dans le plein champ du blé que dans la moutarde et les interfaces blé/bordure. Cette croissance est limitée de manière variable par les communautés adventices présentes dans les compartiments hors champs (bordures). L'analyse de la diversité génétique à l'aide de marqueurs microsatellites de bleuets dans une petite zone agricole montre que les populations sont connectées par des flux de gènes importants. Les barrières écologiques telles que les chemins, semblent être des facteurs de structuration plus importants que la distance géographique séparant les populations. La répartition du bleuet dans le paysage agricole n'est donc pas aléatoire et apparaît dépendante de la fréquence de l'espèce dans la région ainsi que des différents éléments du paysage. Les caractéristiques du cycle biologique du bleuet ont été étudiées grâce à des expériences au champ et en serre. Nous avons montré que la longévité des akènes enfouis dans le sol chute rapidement après deux ans. Le cycle saisonnier de la dormance permet deux cohortes de levées (automne et printemps). L'étude du système de reproduction a permis de mettre en évidence que les pollinisateurs sont nécessaires pour la fécondation et que les populations sont majoritairement auto-incompatibles. Il existe cependant des individus pseudo auto-incompatibles mais leur fréquence n'est pas liée à la taille des populations ou à leur niveau d'isolement spatial. Par ailleurs, dès que le coefficient de consanguinité augmente dans les populations, la valeur phénotypique des individus baisse. La dépression de consanguinité s'exprime alors principalement pendant la phase de la germination. L'ensemble de ces caractéristiques biologiques peuvent s'avérer défavorables au bleuet dans les agroécosystèmes simplifiés actuels et ont pu entraîner son déclin dans certaines régions

Dans cette thèse, nous étudions les sous graphes arc-en-ciel et les sous-graphes correctement colorés dans les graphes à arêtes colorées, et les sous-graphes compatibles dans les graphes avec des systèmes d'incompatibilité, qui peuvent être considérés comme une généralisation des graphes à arêtes colorées. Par rapport aux graphes généraux, les graphes colorés contiennent plus d'informations et sont capables de modéliser des relations plus complexes dans les réseaux de communication, les sciences sociales, la biologie moléculaire, etc. Par conséquent, l'étude des structures dans les graphes aux arêtes colorées est importante à la fois pour la théorie des graphes et pour d'autres sujets connexes. Nous étudions d'abord la condition de degré de couleur minimum forçant les triangles arc-en-ciel à sommets disjoints dans les graphes aux arêtes colorées. En 2013, Li s'est avéré être la meilleure condition de degré de couleur minimum possible pour l'existence d'un triangle arc-en-ciel. Motivés par cela, nous obtenons une condition de degré de couleur minimum précis garantissant l'existence de deux triangles arc-en-ciel à sommets disjoints et proposons une conjecture sur l'existence de k triangles arc-en-ciel à sommets disjoints. Deuxièmement, nous considérons la relation entre l'ordre de l'arbre maximum correctement coloré dans le graphe à bords colorés et le degré de couleur minimum. On obtient que pour un graphe connexe G aux arêtes colorées, l'ordre du maximum d'arbre correctement coloré est au moins \min\{|G|, 2\delta^{c}(G)\}, ce qui généralise un résultat de Cheng, Kano et Wang. De plus, la borne inférieure 2delta^{c}(G) dans notre résultat est la meilleure possible et nous caractérisons tous les graphes extrémaux. Troisièmement, nous recherchons la condition de degré de couleur minimum garantissant l'existence de 2-facteurs correctement colorés dans les graphes aux bords colorés. Nous dérivons une condition de degré de couleur minimum asymptotique forçant chaque facteur 2 correctement coloré avec exactement t composants, ce qui généralise un résultat de Lo. Nous déterminons également la meilleure condition de degré de couleur minimum possible pour l'existence d'un facteur 2 correctement coloré dans un graphe bipartite à arêtes colorées. Enfin, nous étudions les facteurs compatibles dans les graphes avec des systèmes d'incompatibilité. La notion de système d'incompatibilité a été introduite pour la première fois par Krivelevich, Lee et Sudakov, qui peut être considérée comme une mesure quantitative de la robustesse des propriétés du graphe. Récemment, il y a eu un intérêt croissant pour l'étude de la robustesse des propriétés des graphes, visant à renforcer les résultats classiques en théorie des graphes extrémaux et en combinatoire probabiliste. Nous étudions la version robuste du résultat d'Alon-- Yuster par rapport au système d'incompatibilité
In this thesis, we study rainbow subgraphs and properly colored subgraphs in edge-colored graphs, and compatible subgraphs in gra-phs with incompatibility systems, which can be viewed as a generalization of edge-colored graphs. Compared with general graphs, edge-colored gra-phs contain more information and are able to model more complicated relations in communication net-work, social science, molecular biology and so on. Hence, the study of structures in edge-colored graphs is significant to both graph theory and other related subjects. We first study the minimum color degree condition forcing vertex-disjoint rainbow triangles in edge-colored graphs. In 2013, Li proved a best possible minimum color degree condition for the existence of a rainbow triangle. Motivated by this, we obtain a sharp minimum color degree condition guaran-teeing the existence of two vertex-disjoint rainbow triangles and propose a conjecture about the exis-tence of k vertex-disjoint rainbow triangles. Secondly, we consider the relation between the order of maximum properly colored tree in edge-colored graph and the minimum color degree. We obtain that for an edge-colored connected graph G, the order of maximum properly colored tree is at least \min\{|G|, 2\delta^{c}(G)\}, which generalizes a result of Cheng, Kano and Wang. Moreover, the lower bound 2delta^{c}(G) in our result is best possible and we characterize all extremal graphs. Thirdly, we research the minimum color degree condition guaranteeing the existence of properly colored 2-factors in edge-colored graphs. We derive an asymptotic minimum color degree con-dition forcing every properly colored 2-factor with exactly t components, which generalizes a result of Lo. We also determine the best possible mini-mum color degree condition for the existence of a properly colored 2-factor in an edge-colored bipartite graph. Finally, we study compatible factors in graphs with incompatibility systems. The notion of incom-patibility system was firstly introduced by Krivelevich, Lee and Sudakov, which can be viewed as a quantitative measure of robustness of graph properties. Recently, there has been an increasing interest in studying robustness of graph proper-ties, aiming to strengthen classical results in extremal graph theory and probabilistic combina-torics. We study the robust version of Alon--Yuster's result with respect to the incompatibility system

Orientador: Mário Alexandre Coelho Sinhoreti
Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
Made available in DSpace on 2018-08-24T07:19:06Z (GMT). No. of bitstreams: 1 Leme_ArieneArcasTopalPaes_D.pdf: 1762862 bytes, checksum: df60c904446c386b9834989fa6847207 (MD5) Previous issue date: 2013
Resumo: Neste estudo avaliou-se o efeito: (1) da adição de resina de troca aniônica (AER) a um cimento experimental, para reduzir a incompatibilidade entre sistemas adesivos simplificados e cimento resinoso de polimerização dupla, quando ativado quimicamente; (2) da aplicação de uma camada extra de adesivo entre o sistema adesivo simplificado e o cimento resinoso, na resistência da união, propriedades nanomecânicas da camada de cimento e micropermeabilidade na interface adesiva. Discos de resina composta foram cimentados com os sistemas adesivos ACE ALL Bond TE ([ACE] Bisco Inc.) e Scotchbond Universal ([SCU] 3M ESPE) ... Observação: O resumo, na íntegra, poderá ser visualizado no texto completo da tese digital.
Abstract: This study evaluated the effect of: (1) adding anion exchange resin (AER) to an experimental resin cement aiming to reduce incompatibility between simplified-step adhesive systems and dual-cured resin cement when chemically activated; (2) the application of an extra adhesive resin layer between the simplified adhesive system and resin cement, on the microtensile bond strength, nanomechanical properties of the cement layer and micropermeability at the adhesive layer. Resin composite discs were luted with the adhesive systems ACE ALL Bond TE ([ACE] Bisco Inc.) and Scotchbond Universal ([SCU] 3M ESPE) ... Note: The complete abstract is available with the full electronic document
Doutorado
Materiais Dentarios
Doutora em Materiais Dentários

Le système d'incompatibilité végétative a été décrit chez tous des champignons (Eumycètes) comme intervenant dans la limitation des fusions somatiques entre conspécifiques. Chez les champignons la fusion somatique est uniquement possible entre individus de même GCV (Groupe de Compatibilité Végétative). Comme tous les systèmes de reconnaissance du soi, le fonctionnement du système d'incompatibilité végétative des champignons est basé sur une grande diversité allélique. Cette thèse propose d'étudier la relation qui semble exister entre cette diversité des gènes impliqués dans l’incompatibilité végétative des champignons et la pression parasitaire exercée par des éléments cytoplasmiques délétères (ou DCE) transmis lors des fusions somatiques. Trois problématiques ont été abordées, avec trois approches différentes : (1) une approche conceptuelle générale portant sur l’évolution des systèmes de reconnaissance du soi, (2) une approche de modélisation sur le maintien de la diversité en GCV de la population de champignon par un DCE et (3) une approche expérimentale, pour étudier d’une part la perméabilité de la barrière d‘incompatibilité végétative et d’autre part l’interaction C. parasitica/CHV et les liens existant entre transmission et virulence du CHV. Ces études ont permis de montrer l'importance de la perméabilité de la barrière d'incompatibilité végétative à la fois au niveau du maintien de la diversité génétique de la population d'hôte et au niveau de la prévalence des DCE. Il semblerait donc que les DCE évoluent vers des niveaux de virulence faible du fait de la limitation de leur transmission par le système d'incompatibilité végétative de leur hôte. Nos résultats expérimentaux suggèrent que lorsque la diversité en GCV de la population d'hôte est faible, la virulence des DCE pourrait évoluer suivant le modèle du trade-off impliquant une évolution vers un niveau de virulence intermédiaire optimal. Ces travaux permettent donc de mieux comprendre les mécanismes agissant sur l'écologie et l'évolution des interactions champignon/DCE qui, au vu de cette étude, apparaissent comme de bon modèles pour l’étude des systèmes hôtes/parasites
Vegetative incompatibility systems have been described in Fungi as controlling somatic fusion between conspecifics. For fungi, only fungi of the same vc type can fuse together. As other self recognition systems, this system involved high allelic diversity at specific genes. The issue of this work is to study the cause and effect relationship between the evolution of vegetative incompatibility systems and the selective pressure drove by cytoplasmic deleterious elements, transmitted during somatic fusion. Three problematics with three different approach were done : (1) a conceptual general framework on the evolution of self recognition systems (2) a theoretical work on the maintenance of vc type diversity by DCE and (3) an experimental work on the study of relationship between transmission and virulence in the C. parasitica/CHV host-parasite system. Ours results showed the key role of the permeability of the vegetative incompatibility barrier both for vc type diversity maintenance and on DCE prevalence. DCE would evolve toward avirulence in response to the transmission limitation by host incompatibility systems. Experimental work suggested a positive link between virulence and transmission in some population of CHV when host present a low vc type diversity, which could allow the evolution of the DCE toward an intermediate optimal virulence. This study would shed some light on mechanisms acting on the ecology and the evolution of fungi/DCE interaction which, according to our results, would be good study models for works on host-parasite systems

碩士
國立中央大學
環境工程研究所
96
Storing incompatible chemicals can results in great risk. Therefore, determining chemical compatibilities is very important in chemical storage. Traditionally, managers use charts, labels, or simply, texts to do that. But when not enough training or careless occurs, incompatible chemicals can be put together and cause potential risk to accidents. Therefore, developing a system that can automatically identify chemical’s compatibilities is very important. And this research is dedicated to use wireless communication technology to achieve that goal. Basically, the system uses a communication sensing unit (CSU) to talk to other CSU(s) in the same communication fields. If other CSU(s) do exist nearby and is incompatible to it, an audio alarm will be triggered to notice the manager. In this study, we have developed the WAU protocol for the CSU and it has three features. First, the CSU could join or leave the CSU communication groups (CSU Groups) in any time. Second, the CSU could organize themselves by the no-master-and-no-slave communication protocol, and setting communication timeslot by predefined timeframes which is associated with the incompatibility table. This research successful developed the CSU with the size of 67mm * 64mm by using NORDIC nRF24E1 as the radio transceiver. The CSU uses two 1.5v AA batteries to drive buzzer, LED and the radio. And it weights about 100g.Under a thousand simulations in the laboratory, a CSU could communicate with other eight CSUs at least one time in 4.85±4 seconds, and no error has been found this test. Power consumption tests showed that the CSU used 27.5 mA during communication and consumed less than 0.01 mA in power down mode. This means that the CSU can be used continuously for about 200 days at 1% communication density if two 2000mA Ni-MH batteries are used. And it is long enough for many waste chemical storage conditions in campus.

Yes
We report qualitative findings from a study in a multi-ethnic, multi-faith city with high levels of deprivation. Primary research over 2 years consisted of three focus groups and 18 semi-structured interviews with food insecurity service providers followed by focus groups with 16 White British and Pakistani women in or at risk of food insecurity. We consider food insecurity using Habermas’s distinction between the system and lifeworld. We examine system definitions of the nature of need, approved food choices, the reification of selected skills associated with household management and the imposition of a construct of virtue. While lifeworld truths about food insecurity include understandings of structural causes and recognition that the potential of social solidarity to respond to them exist, they are not engaged with by the system. The gap between system rationalities and the experiential nature of lay knowledge generates individual and collective disempowerment and a corrosive sense of shame.
NIHR Collaboration for Leadership in Applied Health Research and Care Yorkshire and Humber (NIHR CLAHRC YH) (Grant Number IS-CLA-0113-10020).

Flowering plants display remarkable phenotypic diversity, especially in reproductive structures, much of which is thought to be associated with pollination by animals. Pollination syndromes are collections of floral traits (e.g. flower colour, shape, odour) that are associated with a plant attracting particular functional groups of animal pollinators. We explored the extent to which traits associated with the moth pollination syndrome translated into pollination by moths in the Pacific coast dune endemic Abronia umbellata and found mixed results: in one year of study, there was no difference in seed set by day- vs. night-pollinated inflorescences, but in another year of study, night-pollinated inflorescences set significantly more seed than those pollinated during the day. We integrate this work with tests of pollen and resource limitation of seed production and with seed set surveys of natural populations to address proximate and ultimate causes of low seed set, finding low rates of pollinator visitation, high pollen limitation of seed production in all populations studied, and no evidence of endogenous resource limitation of seed production. We propose that “excess” flowers may be functionally male, serving to increase outcross siring success. The transition from self-incompatibility and obligate outcrossing to self-compatibility and predominant selfing is the most common evolutionary transition among the flowering plants and traits associated with outcrossing may become reduced across such shifts, potentially through the action of natural selection, especially if pollinators are also herbivores, or if the signals that pollinators use to locate flowers are also used by herbivores. We examined the reduction of attractive visual and olfactory floral traits in A. umbellata across a shift from outcrossing to selfing and found a reduction of all floral traits considered. We found that floral volatile emissions were reduced more strongly than flower size or floral display (number of flowers per inflorescence), but there was no evidence of an ecological cost associated with conspicuousness: we did not find reduced leaf herbivory among selfers relative to outcrossers.
Thesis (Master, Biology) -- Queen's University, 2012-08-30 19:46:23.663

abstract: Isolation-by-distance is a specific type of spatial genetic structure that arises when parent-offspring dispersal is limited. Many natural populations exhibit localized dispersal, and as a result, individuals that are geographically near each other will tend to have greater genetic similarity than individuals that are further apart. It is important to identify isolation-by-distance because it can impact the statistical analysis of population samples and it can help us better understand evolutionary dynamics. For this dissertation I investigated several aspects of isolation-by-distance. First, I looked at how the shape of the dispersal distribution affects the observed pattern of isolation-by-distance. If, as theory predicts, the shape of the distribution has little effect, then it would be more practical to model isolation-by-distance using a simple dispersal distribution rather than replicating the complexities of more realistic distributions. Therefore, I developed an efficient algorithm to simulate dispersal based on a simple triangular distribution, and using a simulation, I confirmed that the pattern of isolation-by-distance was similar to other more realistic distributions. Second, I developed a Bayesian method to quantify isolation-by-distance using genetic data by estimating Wright’s neighborhood size parameter. I analyzed the performance of this method using simulated data and a microsatellite data set from two populations of Maritime pine, and I found that the neighborhood size estimates had good coverage and low error. Finally, one of the major consequences of isolation-by-distance is an increase in inbreeding. Plants are often particularly susceptible to inbreeding, and as a result, they have evolved many inbreeding avoidance mechanisms. Using a simulation, I determined which mechanisms are more successful at preventing inbreeding associated with isolation-by-distance.
Dissertation/Thesis
Doctoral Dissertation Molecular and Cellular Biology 2015

Student Number : 9112588A - MA Dissertation - School of Social Sciences - Faculty of Humanities
There is a concern that causal determinism might render free-will impossible. I compare some different perspectives, namely Compatibilism, Incompatibilism, Libertarianism, and Hard Determinism, and conclude that Hard Determinism is correct—we lack free-will. To further bolster the case, I consider the work of Libet, who has found neuropsychological evidence that our brains non-consciously cause our actions, prior to our being aware of it. Thus we are also not choosing consciously. I then consider Dennett’s work on the role of the conscious self. I defend his model—of a fragmented self—which could not cause our actions. Finally I argue that many things that free-will purportedly provides, eg., justification for the penal system and reactive attitudes, can be reconstructed without free-will. I then end with some speculations about why people still want free-will.