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Relevant bibliographies by topics / In-yeast genome cloning

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Academic literature on the topic 'In-yeast genome cloning'

Author: Grafiati

Published: 8 February 2025

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Journal articles on the topic "In-yeast genome cloning"

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Erickson, J. R., and M. Johnston. "Direct cloning of yeast genes from an ordered set of lambda clones in Saccharomyces cerevisiae by recombination in vivo." Genetics 134, no. 1 (1993): 151–57. http://dx.doi.org/10.1093/genetics/134.1.151.

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Abstract We describe a technique that facilitates the isolation of yeast genes that are difficult to clone. This technique utilizes a plasmid vector that rescues lambda clones as yeast centromere plasmids. The source of these lambda clones is a set of clones whose location in the yeast genome has been determined by L. Riles et al. in 1993. The Escherichia coli-yeast shuttle plasmid carries URA3, ARS4 and CEN6, and contains DNA fragments from the lambda vector that flank the cloned yeast insert. When yeast is cotransformed with linearized plasmid and lambda clone DNA, Ura+ transformants are obt

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Zhang, Jiantao, Zsigmond Benko, Chenyu Zhang, and Richard Y. Zhao. "Advanced Protocol for Molecular Characterization of Viral Genome in Fission Yeast (Schizosaccharomyces pombe)." Pathogens 13, no. 7 (2024): 566. http://dx.doi.org/10.3390/pathogens13070566.

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Fission yeast, a single-cell eukaryotic organism, shares many fundamental cellular processes with higher eukaryotes, including gene transcription and regulation, cell cycle regulation, vesicular transport and membrane trafficking, and cell death resulting from the cellular stress response. As a result, fission yeast has proven to be a versatile model organism for studying human physiology and diseases such as cell cycle dysregulation and cancer, as well as autophagy and neurodegenerative diseases like Alzheimer’s, Parkinson’s, and Huntington’s diseases. Given that viruses are obligate intracel

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Sclafani, Robert A., and Walton L. Fangman. "THYMIDINE UTILIZATION BY tut MUTANTS AND FACILE CLONING OF MUTANT ALLELES BY PLASMID CONVERSION IN S. CEREVISIAE." Genetics 114, no. 3 (1986): 753–67. http://dx.doi.org/10.1093/genetics/114.3.753.

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ABSTRACT Plasmid pJM81 contains a Herpes simplex virus thymidine kinase (TK) gene that is expressed in yeast. Cells containing the plasmid utilize thymidine (TdR) and the analogue 5-bromodeoxyuridine (BUdR) for specific incorporation into DNA. TdR auxotrophs, harboring plasmid pJM81 and a mutation in the yeast gene TMP1 require high concentrations of TdR (300 μg/ml) to support normal growth rates and the wild-type mitochondrial genome (ρ+) cannot be maintained. We have identified a yeast gene, TUT1, in which recessive mutations allow efficient utilization of lower concentrations of TdR. Strain

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Hiltunen, J. K., F. Okubo, V. A. S. Kursu, K. J. Autio, and A. J. Kastaniotis. "Mitochondrial fatty acid synthesis and maintenance of respiratory competent mitochondria in yeast." Biochemical Society Transactions 33, no. 5 (2005): 1162–65. http://dx.doi.org/10.1042/bst0331162.

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Mitochondrial FAS (fatty acid synthesis) of type II is a widely conserved process in eukaryotic organisms, with particular importance for respiratory competence and mitochondrial morphology maintenance in Saccharomyces cerevisiae. The recent characterization of three missing enzymes completes the pathway. Etr1p (enoyl thioester reductase) was identified via purification of the protein followed by molecular cloning. To study the link between FAS and cell respiration further, we also created a yeast strain that has FabI enoyl-ACP (acyl-carrier protein) reductase gene from Escherichia coli engine

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Zhang, Xiao-Ran, Jia-Bei He, Yi-Zheng Wang, and Li-Lin Du. "A Cloning-Free Method for CRISPR/Cas9-Mediated Genome Editing in Fission Yeast." G3: Genes|Genomes|Genetics 8, no. 6 (2018): 2067–77. http://dx.doi.org/10.1534/g3.118.200164.

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Leppert, G., R. McDevitt, S. C. Falco, T. K. Van Dyk, M. B. Ficke, and J. Golin. "Cloning by gene amplification of two loci conferring multiple drug resistance in Saccharomyces." Genetics 125, no. 1 (1990): 13–20. http://dx.doi.org/10.1093/genetics/125.1.13.

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Abstract Yeast DNA fragments that confer multiple drug resistance when amplified were isolated. Cells containing a yeast genomic library cloned in the high copy autonomously replicating vector, YEp24, were plated on medium containing cycloheximide. Five out of 100 cycloheximide-resistant colonies were cross-resistant to the unrelated inhibitor, sulfometuron methyl, due to a plasmid-borne resistance determinant. The plasmids isolated from these resistant clones contained two nonoverlapping regions in the yeast genome now designated PDR4 and PDR5 (for pleiotropic drug resistant). PDR4 was mapped

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Mülleder, Michael, Kate Campbell, Olga Matsarskaia, Florian Eckerstorfer, and Markus Ralser. "Saccharomyces cerevisiae single-copy plasmids for auxotrophy compensation, multiple marker selection, and for designing metabolically cooperating communities." F1000Research 5 (September 20, 2016): 2351. http://dx.doi.org/10.12688/f1000research.9606.1.

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Auxotrophic markers are useful tools in cloning and genome editing, enable a large spectrum of genetic techniques, as well as facilitate the study of metabolite exchange interactions in microbial communities. If unused background auxotrophies are left uncomplemented however, yeast cells need to be grown in nutrient supplemented or rich growth media compositions, which precludes the analysis of biosynthetic metabolism, and which leads to a profound impact on physiology and gene expression. Here we present a series of 23 centromeric plasmids designed to restore prototrophy in typicalSaccharomyce

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Kuspa, A., D. Vollrath, Y. Cheng, and D. Kaiser. "Physical mapping of the Myxococcus xanthus genome by random cloning in yeast artificial chromosomes." Proceedings of the National Academy of Sciences 86, no. 22 (1989): 8917–21. http://dx.doi.org/10.1073/pnas.86.22.8917.

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Hanekamp, Theodor, Mary K. Thorsness, Indrani Rebbapragada, et al. "Maintenance of Mitochondrial Morphology Is Linked to Maintenance of the Mitochondrial Genome in Saccharomyces cerevisiae." Genetics 162, no. 3 (2002): 1147–56. http://dx.doi.org/10.1093/genetics/162.3.1147.

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Abstract In the yeast Saccharomyces cerevisiae, certain mutant alleles of YME4, YME6, and MDM10 cause an increased rate of mitochondrial DNA migration to the nucleus, carbon-source-dependent alterations in mitochondrial morphology, and increased rates of mitochondrial DNA loss. While single mutants grow on media requiring mitochondrial respiration, any pairwise combination of these mutations causes a respiratory-deficient phenotype. This double-mutant phenotype allowed cloning of YME6, which is identical to MMM1 and encodes an outer mitochondrial membrane protein essential for maintaining norm

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Andleeb, S., F. Latif, S. Afzal, Z. Mukhtar, S. Mansoor, and I. Rajoka. "CLONING AND EXPRESSION OF CHAETOMIUM THERMOPHILUM XYLANASE 11-A GENE IN PICHIA PASTORIS." Nucleus 45, no. 1-2 (2020): 75–81. https://doi.org/10.71330/nucleus.45.01-2.1001.

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The various thermophilic fungi like Chaetomium thermophile has potential to secrete xylanase and cellulase enzymes. Inthe present study eukaryotic expression system of Pichia pastoris (yeast) was used to express xylanase gene. Thexylanase (Xyn 11-A) gene was isolated from C. thermophile strain NIBGE-1. Primers were designed to amplify the gene,ligated into P. pastoris pPIC3.5K vector, the resultant recombinant clone pSSZ810 was transformed into the genome ofP. pastoris GS115 strain through electroporation. Transformants were selected on yeast peptone dextrose medium(YPD) plates containing anti

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Dissertations / Theses on the topic "In-yeast genome cloning"

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Barret, Julien. "Clonage, ingénierie et transfert de grands fragments de génome chez Bacillus subtilis." Electronic Thesis or Diss., Bordeaux, 2024. http://www.theses.fr/2024BORD0458.

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L’ingénierie des génomes des micro-organismes est devenue un standard dans les biotechnologies microbiennes. En 2010, des technologies prometteuses de biologie de synthèse utilisant la levure comme plateforme pour l’assemblage et l’ingénierie de génomes synthétiques bactériens suivi de leur transplantation dans une cellule receveuse ont vu le jour. Ces technologies ont conduit à la création des premières cellules synthétiques et ouvert de nouvelles voies vers la construction de cellules aux propriétés biologiques entièrement contrôlées. Le transfert de ces outils à des micro-organismes d’intér

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Book chapters on the topic "In-yeast genome cloning"

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Li, Ge, and Richard Y. Zhao. "Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7546-4_5.

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Benders, Gwynedd A. "Cloning Whole Bacterial Genomes in Yeast." In Methods in Molecular Biology. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-564-0_13.

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Golemis, Erica A., Ilya Serebriiskii, and Susan F. Law. "Adjustment of Parameters in the Yeast Two-Hybrid System." In Gene Cloning and Analysis. Garland Science, 2023. http://dx.doi.org/10.1201/9781003421474-1.

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Baykov, Ivan, Olga Kurchenko, Ekaterina Mikhaylova, Vera V. Morozova, and Nina V. Tikunova. "Robust and Reproducible Protocol for Phage Genome “Rebooting” Using Transformation-Associated Recombination (TAR) Cloning into Yeast Centromeric Plasmid." In Methods in Molecular Biology. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3523-0_19.

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Ougen, P., and D. Cohen. "Yeast artificial chromosomes cloning using PFGE." In Pulsed Field Gel Electrophoresis. Oxford University PressOxford, 1995. http://dx.doi.org/10.1093/oso/9780199635368.003.0005.

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Abstract The major goal of the human genome project includes the isolation of the entire human genome in overlapping clones and the development of physical maps of the cloned DNA. Cloning into yeast artificial chromosomes (YAC) represents the method of choice for genome mapping analysis in the megabase range.

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Anand, Rak Esh. "Cloning into yeast artificial chromosomes." In DNA Cloning 3. Oxford University PressOxford, 1995. http://dx.doi.org/10.1093/oso/9780199634835.003.0004.

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Abstract The analysis of large and complex genomes requires both mapping and cloning of DNA. Until a few years ago the largest fragment of DNA that could be cloned was - 40 kb in length using a cosmid vector (see Chapters I and 2). The development of yeast artificial chromosome (YAC) cloning vectors has greatly extended this cloning range. Following the description of the YAC vector system (1) the size of DNA fragment that could be maintained as a YAC has increased by almost one order of magnitude. This has greatly facilitated the study of complex genomes and has been instrumental in the efforts to construct the first generation physical map of the entire human genome (2). titre plates provides a valuable long-term resource which can be simultaneously accessed by several researchers. Consequently, it is important to plan carefully before embarking on this exercise that requires substantial time and resource. This chapter will mainly concentrate on the construction of a YAC library. For completeness, determination of the average YAC size within the library and a PCR-based library screening method have also been included.

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Dear, Paul H. "Happy mapping." In Genome Mapping. Oxford University PressOxford, 1997. http://dx.doi.org/10.1093/oso/9780199636310.003.0005.

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Abstract Most methods for genome mapping rely on some form of cloning to isolate a subfraction of the genome for analysis, whether into yeast or bacterial hosts (as in physical mapping, Chapters 10 and 11), into hybrid cells (as in radiation hybrid mapping, Chapter 4), or as offspring amongst which polymorphic markers segregate (as in linkage mapping, Chapters 1-3).

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Ivens, Alasdair c., and Peter F. R. Little. "Cosmid clones and their application to genome studies." In DNA Cloning 3. Oxford University PressOxford, 1995. http://dx.doi.org/10.1093/oso/9780199634835.003.0001.

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Abstract A 1000-fold range of DNA sizes may be cloned in the current range of cloning vectors: the ideal vector for genome mapping studies would be one that is easy to use while containing as much DNA as possible. Cosmids now occupy the middle ground: they have a significant capacity, thus reducing the number of steps required to clone an entire gene or region, combined with very simple methods for isolating inserted DNA in pure form. As a consequence, positional cloning strategies frequently involve the use of cosmids as a final cloning vector for reducing a yeast artificial chromosome (YAC) clone to manageably sized DNA fragments (1). Cosmids are units that are very likely to contain an entire gene, are easily mapped with respect to restriction sites, and are amenable to the application of a number of other functional assays, e.g. exon trapping (2, 3), genomic sequencing (4-6), and fingerprinting to generate contig maps (7, 8). As a result, the detailed information that can be obtained from a cosmid clone makes it the ideal medium for genome analysis. Indeed, several genome mapping studies (e.g, Caenorhabditis elegans (9), Escherichia coli (10)), have relied on physical DNA maps built around cosmid clones.

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Sikorski, Roberts, Jill B. Keeney,, and Jef D. Boeke. "Plasmid shuffling and mutant isolation." In Molecular Genetics of Yeast. Oxford University PressOxford, 1992. http://dx.doi.org/10.1093/oso/9780199634309.003.0006.

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Abstract Genetic analysis of mutants in Saccharomyces cerev1szae offers a unique approach to the study of biological processes. To initiate genetic studies in this organism one can mutagenize a population and screen or select for those mutants which affect the process of interest. Alternatively, one can use techniques selectively to mutagenize a single gene already known to play an important role in this process. The generation of mutant yeast strains starting from a cloned non-essential yeast gene is relatively straightforward. To remove the wild-type gene product, an essential step in analysing recessive alleles, DNA at the wild-type non-essential locus can be deleted entirely from the genome. A collection of mutant alleles can be made in vitro and introduced into the deleted host cell via episomal cloning vectors. The generation of mutant yeast strains from an essential yeast gene is a more complicated task since removal of the wild-type gene in one simple step yields an inviable genotype.

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Newman, Andrew. "Analysis of pre-mRNA splicing in yeast." In RNA Processing. Oxford University PressOxford, 1994. http://dx.doi.org/10.1093/oso/9780199633449.003.0006.

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Abstract Many simple but powerful techniques have been developed to investigate gene expression in Saccharomyces cerevisiae, and a number of these have been employed in the analysis of RNA splicing. Test substrates can be transcribed from expression cassettes introduced into yeast by transformation or transplacement, and splicing can be monitored by RNA analysis or assay of a suitable reporter gene product. Gene disruption and inducible expression systems have been invaluable for investigating the roles of components of the splicing machinery in yeast. Many of the genes for yeast splicing factors have recently been isolated by molecular cloning, which is facilitated by the compact nature of the Saccharomyces genome and the fact that these genes are present as single copies. Splicing of yeast mRNA precursors can also be studied in vitro, since for Saccharomyces a simple method has been developed for making cell-free extracts capable of splicing synthetic pre-mRNA substrates. Such studies have shown that the splicing pathway in yeast is very similar to that of higher eukaryotes and that there is considerable conservation of structure and function between splicing factors in yeast and mammalian cells. However, yeast is an extremely valuable system for the detailed analysis of splicing since it allows some approaches which are not possible with mammalian cells, particularly the genetic selection and isolation of splicing mutants.

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Reports on the topic "In-yeast genome cloning"

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Droby, Samir, Michael Wisniewski, Martin Goldway, Wojciech Janisiewicz, and Charles Wilson. Enhancement of Postharvest Biocontrol Activity of the Yeast Candida oleophila by Overexpression of Lytic Enzymes. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7586481.bard.

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Enhancing the activity of biocontrol agents could be the most important factor in their success in controlling fruit disease and their ultimate acceptance in commercial disease management. Direct manipulation of a biocontrol agent resulting in enhancement of diseases control could be achieved by using recent advances in molecular biology techniques. The objectives of this project were to isolate genes from yeast species that were used as postharvest biocontrol agents against postharvest diseases and to determine their role in biocontrol efficacy. The emphasis was to be placed on the yeast, Can

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Wagner, D. Ry, Eliezer Lifschitz, and Steve A. Kay. Molecular Genetic Analysis of Flowering in Arabidopsis and Tomato. United States Department of Agriculture, 2002. http://dx.doi.org/10.32747/2002.7585198.bard.

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The primary objectives for the US lab included: the characterization of ELF3 transcription and translation; the creation and characterization of various transgenic lines that misexpress ELF3; defining genetic pathways related to ELF3 function regulating floral initiation in Arabidopsis; and the identification of genes that either interact with or are regulated by ELF3. Light quality, photoperiod, and temperature often act as important and, for some species, essential environmental cues for the initiation of flowering. However, there is relatively little information on the molecular mechanisms

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