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Journal articles on the topic 'In vivo probing'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Mitchell, David, Sarah M. Assmann, and Philip C. Bevilacqua. "Probing RNA structure in vivo." Current Opinion in Structural Biology 59 (December 2019): 151–58. http://dx.doi.org/10.1016/j.sbi.2019.07.008.

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2

McElroy, Gregory S., and Navdeep S. Chandel. "Probing mitochondrial metabolism in vivo." Proceedings of the National Academy of Sciences 116, no. 1 (December 18, 2018): 20–22. http://dx.doi.org/10.1073/pnas.1819614116.

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3

Wirtz, D., and B. R. Daniels. "Probing single cell micromechanics in vivo." Journal of Biomechanics 39 (January 2006): S588. http://dx.doi.org/10.1016/s0021-9290(06)85438-2.

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4

Burgess, Darren J. "Detailed probing of RNA structure in vivo." Nature Reviews Genetics 16, no. 5 (April 9, 2015): 255. http://dx.doi.org/10.1038/nrg3939.

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5

Sasse-Dwight, Selina, and Jay D. Gralla. "Probing co-operative DNA-binding in vivo." Journal of Molecular Biology 202, no. 1 (July 1988): 107–19. http://dx.doi.org/10.1016/0022-2836(88)90523-2.

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6

Xin, Li, Sun Yanan, and Wang Xiaochen. "Probing lysosomal activity in vivo." Biophysics Reports 7, no. 1 (2021): 1–7. http://dx.doi.org/10.52601/bpr.2021.200047.

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7

Valenta, T., M. Gay, S. Steiner, K. Draganova, M. Zemke, R. Hoffmans, P. Cinelli, M. Aguet, L. Sommer, and K. Basler. "Probing transcription-specific outputs of -catenin in vivo." Genes & Development 25, no. 24 (December 15, 2011): 2631–43. http://dx.doi.org/10.1101/gad.181289.111.

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8

McDonald, Beth M., Mateusz M. Wydro, Robert N. Lightowlers, and Jeremy H. Lakey. "Probing the orientation of yeast VDAC1 in vivo." FEBS Letters 583, no. 4 (January 29, 2009): 739–42. http://dx.doi.org/10.1016/j.febslet.2009.01.039.

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9

Radbruch, Helena, Daniel Bremer, Ronja Mothes, Robert Günther, Jan Rinnenthal, Julian Pohlan, Carolin Ulbricht, Anja Hauser, and Raluca Niesner. "Intravital FRET: Probing Cellular and Tissue Function in Vivo." International Journal of Molecular Sciences 16, no. 12 (May 21, 2015): 11713–27. http://dx.doi.org/10.3390/ijms160511713.

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10

Fujiwara, Masazumi, Simo Sun, Alexander Dohms, Yushi Nishimura, Ken Suto, Yuka Takezawa, Keisuke Oshimi, et al. "Real-time nanodiamond thermometry probing in vivo thermogenic responses." Science Advances 6, no. 37 (September 2020): eaba9636. http://dx.doi.org/10.1126/sciadv.aba9636.

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Real-time temperature monitoring inside living organisms provides a direct measure of their biological activities. However, it is challenging to reduce the size of biocompatible thermometers down to submicrometers, despite their potential applications for the thermal imaging of subtissue structures with single-cell resolution. Here, using quantum nanothermometers based on optically accessible electron spins in nanodiamonds, we demonstrate in vivo real-time temperature monitoring inside Caenorhabditis elegans worms. We developed a microscope system that integrates a quick-docking sample chamber, particle tracking, and an error correction filter for temperature monitoring of mobile nanodiamonds inside live adult worms with a precision of ±0.22°C. With this system, we determined temperature increases based on the worms’ thermogenic responses during the chemical stimuli of mitochondrial uncouplers. Our technique demonstrates the submicrometer localization of temperature information in living animals and direct identification of their pharmacological thermogenesis, which may allow for quantification of their biological activities based on temperature.

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Brindle, K., S. Fulton, J. Sheldon, and S. Williams. "Probing the properties of enzymes in vivo using NMR." Biochemical Society Transactions 23, no. 2 (May 1, 1995): 376–81. http://dx.doi.org/10.1042/bst0230376.

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12

Wittke, Sandra, Nicole Lewke, Silke Müller, and Nils Johnsson. "Probing the Molecular Environment of Membrane Proteins In Vivo." Molecular Biology of the Cell 10, no. 8 (August 1999): 2519–30. http://dx.doi.org/10.1091/mbc.10.8.2519.

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The split-Ubiquitin (split-Ub) technique was used to map the molecular environment of a membrane protein in vivo. Cub, the C-terminal half of Ub, was attached to Sec63p, and Nub, the N-terminal half of Ub, was attached to a selection of differently localized proteins of the yeast Saccharomyces cerevisiae. The efficiency of the Nub and Cub reassembly to the quasi-native Ub reflects the proximity between Sec63-Cub and the Nub-labeled proteins. By using a modified Ura3p as the reporter that is released from Cub, the local concentration between Sec63-Cub-RUra3p and the different Nub-constructs could be translated into the growth rate of yeast cells on media lacking uracil. We show that Sec63p interacts with Sec62p and Sec61p in vivo. Ssh1p is more distant to Sec63p than its close sequence homologue Sec61p. Employing Nub- and Cub-labeled versions of Ste14p, an enzyme of the protein isoprenylation pathway, we conclude that Ste14p is a membrane protein of the ER. Using Sec63p as a reference, a gradient of local concentrations of different t- and v-SNARES could be visualized in the living cell. The RUra3p reporter should further allow the selection of new binding partners of Sec63p and the selection of molecules or cellular conditions that interfere with the binding between Sec63p and one of its known partners.

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13

Kwok, Chun Kit. "Dawn of the in vivo RNA structurome and interactome." Biochemical Society Transactions 44, no. 5 (October 15, 2016): 1395–410. http://dx.doi.org/10.1042/bst20160075.

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RNA is one of the most fascinating biomolecules in living systems given its structural versatility to fold into elaborate architectures for important biological functions such as gene regulation, catalysis, and information storage. Knowledge of RNA structures and interactions can provide deep insights into their functional roles in vivo. For decades, RNA structural studies have been conducted on a transcript-by-transcript basis. The advent of next-generation sequencing (NGS) has enabled the development of transcriptome-wide structural probing methods to profile the global landscape of RNA structures and interactions, also known as the RNA structurome and interactome, which transformed our understanding of the RNA structure–function relationship on a transcriptomic scale. In this review, molecular tools and NGS methods used for RNA structure probing are presented, novel insights uncovered by RNA structurome and interactome studies are highlighted, and perspectives on current challenges and potential future directions are discussed. A more complete understanding of the RNA structures and interactions in vivo will help illuminate the novel roles of RNA in gene regulation, development, and diseases.

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Inoue, Shogo, Hiroaki Shiina, Yozo Mitsui, Hiroaki Yasumoto, Akio Matsubara, and Mikio Igawa. "Identification of lymphatic pathway involved in the spread of bladder cancer: Evidence obtained from fluorescence navigation with intraoperatively injected indocyanine green." Canadian Urological Association Journal 7, no. 5-6 (May 13, 2013): 322. http://dx.doi.org/10.5489/cuaj.1251.

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Introduction: We identify lymphatic vessels draining from the bladderby using fluorescence navigation (FN) system.Methods: In total, 12 candidates for radical cystectomy and pelviclymph node dissection (PLND) were included in this study. Afteran indocyanine green (ICG) solution was injected into the bladderduring radical cystectomy, lymphatic vessels draining from thebladder were analyzed using a FN system. PLND was based onthe lymphatic mapping created from the FN measurements (in vivoprobing) in the external iliac, obturator and internal iliac regions;after PLND, the fluorescence of the removed lymph nodes (LNs)was analyzed on the bench (ex vivo probing).Results: There were no patients with complications associated withthe intravesical ICG injection. A lymphatic pathway along inferiorvesical vessels to internal iliac LNs was clearly illustrated in 7 cases.Under in-vivo probing, the fluorescence intensity of internal iliacnodes was greater than that of external iliac or obturator nodes.Under ex-vivo probing, the fluorescence intensity of internal iliacand obturator nodes was greater than that of external iliac nodes.Conclusions: Using an FN system after injecting ICG during a radicalcystectomy operation is a safe and rational approach to detectingthe lymphatic channel draining from the bladder.

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15

Irimia, Daniel, and Xiao Wang. "Inflammation-on-a-Chip: Probing the Immune System Ex Vivo." Trends in Biotechnology 36, no. 9 (September 2018): 923–37. http://dx.doi.org/10.1016/j.tibtech.2018.03.011.

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16

Liu, Ben, Jingwei Ni, and Maurille J. Fournier. "Probing RNA in Vivo with Methylation Guide Small Nucleolar RNAs." Methods 23, no. 3 (March 2001): 276–86. http://dx.doi.org/10.1006/meth.2000.1138.

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17

Sasse-Dwight, S., and J. D. Gralla. "Probing the Escherichia coli glnALG upstream activation mechanism in vivo." Proceedings of the National Academy of Sciences 85, no. 23 (December 1, 1988): 8934–38. http://dx.doi.org/10.1073/pnas.85.23.8934.

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18

Nijenhuis, Nadja, Marcel Bremerich, Hans Vink, Jos A. E. Spaan, and Christoph Schmidt. "The Optical Mouse Trap: in Vivo Probing of Capillary Viscoelasticity." Biophysical Journal 98, no. 3 (January 2010): 368a. http://dx.doi.org/10.1016/j.bpj.2009.12.1986.

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19

Tormos, Kathryn V., and Navdeep S. Chandel. "Seeing the Light: Probing ROS In Vivo Using Redox GFP." Cell Metabolism 14, no. 6 (December 2011): 720–21. http://dx.doi.org/10.1016/j.cmet.2011.11.008.

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20

Sapoznikov, Anita, and Steffen Jung. "Probing in vivo dendritic cell functions by conditional cell ablation." Immunology & Cell Biology 86, no. 5 (April 15, 2008): 409–15. http://dx.doi.org/10.1038/icb.2008.23.

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21

Sokoloff, Louis. "In Vivo Veritas: Probing Brain Function Through the Use of Quantitative In Vivo Biochemical Techniques." Annual Review of Physiology 62, no. 1 (March 2000): 1–24. http://dx.doi.org/10.1146/annurev.physiol.62.1.1.

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22

Reinoehl, R. Bruce, and James W. Halle. "Increasing the Assessment Probe Performance of Teacher Aides through Written Prompts." Journal of the Association for Persons with Severe Handicaps 19, no. 1 (March 1994): 32–42. http://dx.doi.org/10.1177/154079699401900104.

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Two supervisory procedures for promoting the conduct of in vivo probes by six special education teacher aides were examined. The first procedure served as baseline and consisted of inservice training, self-regulated probing and data recording, and incidental modeling by the primary investigator. This procedure produced poor probing performance, reflecting inconsistency, low frequency, and differential sensitivity toward the three participating students. The second procedure consisted of the addition of delivering data cards to aides, thus prompting them to conduct daily probes. This additional component resulted in a 53% increase in the level of probing and was accompanied by less variability, higher sustained rates of probing, and more equitable probing of the students. Two types of reversal probes produced evidence that (a) the investigator's absence and (b) his presence without delivering cards occasioned low performance levels. Both conceptual (stimulus-control analysis) and applied (aides' preference for students) implications are discussed.

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23

Satyanarayana, KV, BR Anuradha, G. Srikanth, P. Mohan Chandra, T. Anupama, and M. Prasad Durga. "Clinical Evaluation of Intrabony Defects in Localized Aggressive Periodontitis Patients with and without Bioglass- An In-vivo Study." Kathmandu University Medical Journal 10, no. 1 (October 2, 2012): 7–10. http://dx.doi.org/10.3126/kumj.v10i1.6906.

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Background Aggressive periodontitis is a specific type of periodontitis with clearly identifiable clinical characteristics such as “rapid attachment loss, bone destruction” and “familial aggregation”. Regeneration of mineralized tissues affected by aggressive periodontitis comprises a major scientific and clinical challenge. In recent years some evidence has been provided that bioactive glass is also capable of supporting the regenerative healing of periodontal lesions. Objective The aim of this clinical and radiological prospective study was to evaluate the efficacy of bioactive glass in the treatment of intra-bony defects in patients with localized aggressive periodontitis. Methods Twelve localized aggressive periodontitis patients with bilaterally located three-walled intra-bony defect depth ? 2 mm, preoperative probing depths ? 5 mm were randomly treated either with the bioactive glass or without the bioactive glass. The clinical parameters plaque index, gingival index, probing depth, gingival recession, clinical attachment level, and mobility were recorded prior to surgery as well as 12 months after surgery. Intraoral radiographs were digitized to evaluate the bone defect depth at baseline and 12 months after the surgery. Results After 12 months, a reduction in probing depth of 3.92 + 0.313 mm (P <0.001) and a gain in clinical attachment level of 4.42+0358mm (P <0.001) were registered in the test group. In the control group, a reduction in probing depth of 2.5 +0.230mm (P <0.001) and a gain in clinical attachment level of 2.58 + 0.149 mm (P<0.001) was recorded. Radiographically, the defects were found to be filled by 2.587 + 0.218 mm (P <0.001) in the test group and by 0.1792 + 0.031mm (P <0.001) in the control group. Changes in gingival recession showed no significant differences. . Conclusion Highly significant improvements in the parameters Probing depth, Clinical attachment level, and Bone defect depth were recorded after 12 months, with regenerative material. KATHMANDU UNIVERSITY MEDICAL JOURNAL VOL.10 | NO. 1 | ISSUE 37 | JAN - MAR 2012 | 11-15 DOI: http://dx.doi.org/10.3126/kumj.v10i1.6906

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24

Wu, Zhaofa, and Yulong Li. "New frontiers in probing the dynamics of purinergic transmitters in vivo." Neuroscience Research 152 (March 2020): 35–43. http://dx.doi.org/10.1016/j.neures.2020.01.008.

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25

Kostesha, Natalie V., João R. M. Almeida, Arto R. Heiskanen, Marie F. Gorwa-Grauslund, Barbel Hahn-Hägerdal, and Jenny Emnéus. "Electrochemical Probing of in Vivo 5-Hydroxymethyl Furfural Reduction inSaccharomyces cerevisiae." Analytical Chemistry 81, no. 24 (December 15, 2009): 9896–901. http://dx.doi.org/10.1021/ac901402m.

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26

Bevilacqua, Philip C., and Sarah M. Assmann. "Technique Development for Probing RNA Structure In Vivo and Genome-Wide." Cold Spring Harbor Perspectives in Biology 10, no. 10 (October 2018): a032250. http://dx.doi.org/10.1101/cshperspect.a032250.

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27

Cabrita, Lisa D., Shang-Te Danny Hsu, Helene Launay, Christopher M. Dobson, and John Christodoulou. "Probing ribosome-nascent chain complexes produced in vivo by NMR spectroscopy." Proceedings of the National Academy of Sciences 106, no. 52 (December 17, 2009): 22239–44. http://dx.doi.org/10.1073/pnas.0903750106.

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28

Chaiken, J., Rebecca J. Bussjager, George Shaheen, David Rice, Dave Stehlik, and John Fayos. "Instrument for near infrared emission spectroscopic probing of human fingertipsin vivo." Review of Scientific Instruments 81, no. 3 (March 2010): 034301. http://dx.doi.org/10.1063/1.3314290.

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29

Nucciotti, V., C. Stringari, L. Sacconi, F. Vanzi, L. Fusi, M. Linari, G. Piazzesi, V. Lombardi, and F. S. Pavone. "Probing myosin structural conformation in vivo by second-harmonic generation microscopy." Proceedings of the National Academy of Sciences 107, no. 17 (April 12, 2010): 7763–68. http://dx.doi.org/10.1073/pnas.0914782107.

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30

Twittenhoff, Christian, Vivian B. Brandenburg, Francesco Righetti, Aaron M. Nuss, Axel Mosig, Petra Dersch, and Franz Narberhaus. "Lead-seq: transcriptome-wide structure probing in vivo using lead(II) ions." Nucleic Acids Research 48, no. 12 (May 28, 2020): e71-e71. http://dx.doi.org/10.1093/nar/gkaa404.

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Abstract The dynamic conformation of RNA molecules within living cells is key to their function. Recent advances in probing the RNA structurome in vivo, including the use of SHAPE (Selective 2′-Hydroxyl Acylation analyzed by Primer Extension) or kethoxal reagents or DMS (dimethyl sulfate), provided unprecedented insights into the architecture of RNA molecules in the living cell. Here, we report the establishment of lead probing in a global RNA structuromics approach. In order to elucidate the transcriptome-wide RNA landscape in the enteric pathogen Yersinia pseudotuberculosis, we combined lead(II) acetate-mediated cleavage of single-stranded RNA regions with high-throughput sequencing. This new approach, termed ‘Lead-seq’, provides structural information independent of base identity. We show that the method recapitulates secondary structures of tRNAs, RNase P RNA, tmRNA, 16S rRNA and the rpsT 5′-untranslated region, and that it reveals global structural features of mRNAs. The application of Lead-seq to Y. pseudotuberculosis cells grown at two different temperatures unveiled the first temperature-responsive in vivo RNA structurome of a bacterial pathogen. The translation of candidate genes derived from this approach was confirmed to be temperature regulated. Overall, this study establishes Lead-seq as complementary approach to interrogate intracellular RNA structures on a global scale.

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Beard, Rhiannon, Nisha Singh, Christophe Grundschober, Antony D. Gee, and Edward W. Tate. "High-yielding18F radiosynthesis of a novel oxytocin receptor tracer, a probe for nose-to-brain oxytocin uptakein vivo." Chemical Communications 54, no. 58 (2018): 8120–23. http://dx.doi.org/10.1039/c8cc01400k.

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32

Tan, Yiqun, Min Liu, Junkuo Gao, Jiancan Yu, Yuanjing Cui, Yu Yang, and Guodong Qian. "A new fluorescent probe for Zn2+ with red emission and its application in bioimaging." Dalton Trans. 43, no. 21 (2014): 8048–53. http://dx.doi.org/10.1039/c4dt00167b.

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Selectively probing Zn²⁺in vivo! A new fluorescent probe highly sensitive and selective for Zn²⁺ based on the ICT effect was designed. This probe showed potential application in biology.

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33

Zou, Xianmei, Yi Liu, Xingjun Zhu, Min Chen, Liming Yao, Wei Feng, and Fuyou Li. "An Nd3+-sensitized upconversion nanophosphor modified with a cyanine dye for the ratiometric upconversion luminescence bioimaging of hypochlorite." Nanoscale 7, no. 9 (2015): 4105–13. http://dx.doi.org/10.1039/c4nr06407k.

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An Nd³⁺-sensitized upconversion nanosystem was successfully used as a high-contrast nanoprobe for probing ClO⁻ in living cells and as an in vivo mouse model of arthritis under 808 nm irradiation.

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34

Rotenberg, Menahem Y., Naomi Yamamoto, Erik N. Schaumann, Laura Matino, Francesca Santoro, and Bozhi Tian. "Living myofibroblast–silicon composites for probing electrical coupling in cardiac systems." Proceedings of the National Academy of Sciences 116, no. 45 (October 17, 2019): 22531–39. http://dx.doi.org/10.1073/pnas.1913651116.

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Traditional bioelectronics, primarily comprised of nonliving synthetic materials, lack cellular behaviors such as adaptability and motility. This shortcoming results in mechanically invasive devices and nonnatural signal transduction across cells and tissues. Moreover, resolving heterocellular electrical communication in vivo is extremely limited due to the invasiveness of traditional interconnected electrical probes. In this paper, we present a cell–silicon hybrid that integrates native cellular behavior (e.g., gap junction formation and biosignal processing) with nongenetically enabled photosensitivity. This hybrid configuration allows interconnect-free cellular modulation with subcellular spatial resolution for bioelectric studies. Specifically, we hybridize cardiac myofibroblasts with silicon nanowires and use these engineered hybrids to synchronize the electrical activity of cardiomyocytes, studying heterocellular bioelectric coupling in vitro. Thereafter, we inject the engineered myofibroblasts into heart tissues and show their ability to seamlessly integrate into contractile tissues in vivo. Finally, we apply local photostimulation with high cell specificity to tackle a long-standing debate regarding the existence of myofibroblast–cardiomyocyte electrical coupling in vivo.

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Hananouchi, Takehito, Soshi Uchida, Yusuke Hashimoto, Funakoshi Noboru, and Stephen K. Aoki. "Comparison of Labrum Resistance Force while Pull-Probing In Vivo and Cadaveric Hips." Biomimetics 6, no. 2 (May 31, 2021): 35. http://dx.doi.org/10.3390/biomimetics6020035.

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Cadaver tissue has been identified as the highest-fidelity anatomical representation in terms of the training for orthopedic surgery, including for arthroscopy of a damaged hip labrum. However, hip labrum stiffness in vivo and in cadavers has not been directly compared. The purpose of this study was to compare in vivo and cadaveric hip labrum stiffness during pull-probing with a force sensor. We measured the resistance force of the hip labrum in ten patients during hip arthroscopy (i.e., in vivo) and compared it with ten cadavers, both intact and detached from the acetabulum, using a surgical knife. We confirmed a partial labral tear (i.e., not detached fully from the rim) at an antero-superior potion in all of the patients. The mean highest resistance levels for the hip labrum in the patients (4.7 N) were significantly lower than the intact cadaveric labrum (8.3 N), and slightly higher than the detached labrum (4.2 N). In this study, the stiffness of the cadaveric labrum tissue was similar to that of the in-vivo hip labrum.

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Bosisio, Mattéo R., Corentin Maisonneuve, Sylvie Gregoire, Adrian Kettaneh, Christopher G. Mueller, and S. Lori Bridal. "Ultrasound Biomicroscopy: A Powerful Tool Probing Murine Lymph Node Size in vivo." Ultrasound in Medicine & Biology 35, no. 7 (July 2009): 1209–16. http://dx.doi.org/10.1016/j.ultrasmedbio.2009.02.005.

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37

Chaiken, Joseph, and Jerry Goodisman. "On probing human fingertips in vivo using near-infrared light: model calculations." Journal of Biomedical Optics 15, no. 3 (2010): 037007. http://dx.doi.org/10.1117/1.3431119.

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Girven, Kasey S., and Dennis R. Sparta. "Probing Deep Brain Circuitry: New Advances in in Vivo Calcium Measurement Strategies." ACS Chemical Neuroscience 8, no. 2 (February 2, 2017): 243–51. http://dx.doi.org/10.1021/acschemneuro.6b00307.

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Kneipp, Janina, Harald Kneipp, Margaret McLaughlin, Dennis Brown, and Katrin Kneipp. "In Vivo Molecular Probing of Cellular Compartments with Gold Nanoparticles and Nanoaggregates." Nano Letters 6, no. 10 (October 2006): 2225–31. http://dx.doi.org/10.1021/nl061517x.

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40

Zubradt, Meghan, Paromita Gupta, Sitara Persad, Alan M. Lambowitz, Jonathan S. Weissman, and Silvi Rouskin. "DMS-MaPseq for genome-wide or targeted RNA structure probing in vivo." Nature Methods 14, no. 1 (November 7, 2016): 75–82. http://dx.doi.org/10.1038/nmeth.4057.

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Tian, Lijin, Shazia Farooq, and Herbert van Amerongen. "Probing the picosecond kinetics of the photosystem II core complex in vivo." Physical Chemistry Chemical Physics 15, no. 9 (2013): 3146. http://dx.doi.org/10.1039/c3cp43813a.

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42

Incarnato, Danny, Edoardo Morandi, Francesca Anselmi, Lisa M. Simon, Giulia Basile, and Salvatore Oliviero. "In vivo probing of nascent RNA structures reveals principles of cotranscriptional folding." Nucleic Acids Research 45, no. 16 (July 14, 2017): 9716–25. http://dx.doi.org/10.1093/nar/gkx617.

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43

IMPERATO, ASSUNTA, MARIA G. SCROCCO, ORLANDO GHIRARDI, MARIA T. RAMACCI, and LUCIANO ANGELUCCI. "In Vivo Probing of the Brain Cholinergic System in the Aged Rat." Annals of the New York Academy of Sciences 621, no. 1 Physiological (July 1991): 90–97. http://dx.doi.org/10.1111/j.1749-6632.1991.tb16971.x.

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44

Erckens, R. "Confocal in vivo probing of proteins and water in the rabbit cornea." Vision Research 35, no. 1 (October 1995): S136. http://dx.doi.org/10.1016/0042-6989(95)98512-8.

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45

Cecioni, Samy, and David J. Vocadlo. "Tools for probing and perturbing O-GlcNAc in cells and in vivo." Current Opinion in Chemical Biology 17, no. 5 (October 2013): 719–28. http://dx.doi.org/10.1016/j.cbpa.2013.06.030.

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46

Wang, Zi Hao, Xue Feng Wang, Han Jiang, Jing Ding, Jian Dong Wang, and Wei Bin Shi. "Probing Near-Infrared Quantum Dots for Imaging and Biomedical Applications." Advanced Materials Research 345 (September 2011): 3–11. http://dx.doi.org/10.4028/www.scientific.net/amr.345.3.

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As light-emitting nanocrystals, quantum dots (QDs) have created a new realm of bioscience by combining nanomaterials with biology. They also have been a major focus of research and development during the past decade, which will profoundly influence future biological as well as biomedical research. In recent years, near-infrared (NIR) quantum dots have emerged in analytical applications, especially for in vitro and in vivo imaging. The impetus behind such endeavors can be attributed to their unique optical and chemical properties, with size-tunable light emission, high photo stability, and manifold fluorescence colors. In this review, we focus on fluorescent imaging with near-infrared (NIR) quantum dots (QDs) both in vitro and in vivo, and the advantages of QDs and potential problems to their use in practical biomedical applications. The ultimate targets aim at decreasing the cytotoxicity of QDs and the future outlook of QD applications in biomedical fields.

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47

Mojumdar, Enamul Haque, Lone Bruhn Madsen, Henri Hansson, Ida Taavoniku, Klaus Kristensen, Christina Persson, Anna Karin Morén, et al. "Probing Skin Barrier Recovery on Molecular Level Following Acute Wounds: An In Vivo/Ex Vivo Study on Pigs." Biomedicines 9, no. 4 (March 31, 2021): 360. http://dx.doi.org/10.3390/biomedicines9040360.

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Proper skin barrier function is paramount for our survival, and, suffering injury, there is an acute need to restore the lost barrier and prevent development of a chronic wound. We hypothesize that rapid wound closure is more important than immediate perfection of the barrier, whereas specific treatment may facilitate perfection. The aim of the current project was therefore to evaluate the quality of restored tissue down to the molecular level. We used Göttingen minipigs with a multi-technique approach correlating wound healing progression in vivo over three weeks, monitored by classical methods (e.g., histology, trans-epidermal water loss (TEWL), pH) and subsequent physicochemical characterization of barrier recovery (i.e., small and wide-angle X-ray diffraction (SWAXD), polarization transfer solid-state NMR (PTssNMR), dynamic vapor sorption (DVS), Fourier transform infrared (FTIR)), providing a unique insight into molecular aspects of healing. We conclude that although acute wounds sealed within two weeks as expected, molecular investigation of stratum corneum (SC) revealed a poorly developed keratin organization and deviations in lipid lamellae formation. A higher lipid fluidity was also observed in regenerated tissue. This may have been due to incomplete lipid conversion during barrier recovery as glycosphingolipids, normally not present in SC, were indicated by infrared FTIR spectroscopy. Evidently, a molecular approach to skin barrier recovery could be a valuable tool in future development of products targeting wound healing.

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Soares, Priscilla Barbosa Ferreira, Helder Henrique Machado de Menezes, Marina de Melo Naves, Eulázio Mikio Taga, and Denildo de Magalhães. "Effect of absorbent tetracycline-loaded membrane used in the reduction of periodontal pockets: an in vivo study." Brazilian Dental Journal 20, no. 5 (2009): 414–18. http://dx.doi.org/10.1590/s0103-64402009000500010.

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This clinical study evaluated the influence of scaling and root planning (SRP), with and without the use of tetracycline-loaded bovine absorbent membrane, in the reduction of periodontal pockets according to 3 parameters: probing pocket depth (PPD), bleeding on probing (BOP) and plaque index (PI). Twenty-four patients were selected totalizing 144 random teeth divided in 2 groups (n=72 teeth) - control (SRP) and experimental (SRP with tetracycline-loaded absorbent membrane). PPD, BOP and PI were determined before and 28 days after the treatment. In all patients, the PPD values at the end of the treatment were always lower than the baseline values. There was a reduction of the PI for both treatments, but it was more evident on the experimental group. In conclusion, the use of tetracycline-loaded absorbent membrane could result in a better prognosis compared to scaling and root planning after only 28 days of evaluation.

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Göbel, Werner, and Fritjof Helmchen. "New Angles on Neuronal Dendrites In Vivo." Journal of Neurophysiology 98, no. 6 (December 2007): 3770–79. http://dx.doi.org/10.1152/jn.00850.2007.

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Imaging technologies are well suited to study neuronal dendrites, which are key elements for synaptic integration in the CNS. Dendrites are, however, frequently oriented perpendicular to tissue surfaces, impeding in vivo imaging approaches. Here we introduce novel laser-scanning modes for two-photon microscopy that enable in vivo imaging of spatiotemporal activity patterns in dendrites. First, we developed a method to image planes arbitrarily oriented in 3D, which proved particularly beneficial for calcium imaging of parallel fibers and Purkinje cell dendrites in rat cerebellar cortex. Second, we applied free linescans—either through multiple dendrites or along a single vertically oriented dendrite—to reveal fast dendritic calcium dynamics in neocortical pyramidal neurons. Finally, we invented a ribbon-type 3D scanning method for imaging user-defined convoluted planes enabling simultaneous measurements of calcium signals along multiple apical dendrites. These novel scanning modes will facilitate optical probing of dendritic function in vivo.

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Wolak, Daniel J., Michelle E. Pizzo, and Robert G. Thorne. "Probing the extracellular diffusion of antibodies in brain using in vivo integrative optical imaging and ex vivo fluorescence imaging." Journal of Controlled Release 197 (January 2015): 78–86. http://dx.doi.org/10.1016/j.jconrel.2014.10.034.

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