Academic literature on the topic 'In vivo probing'

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Journal articles on the topic "In vivo probing"

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Mitchell, David, Sarah M. Assmann, and Philip C. Bevilacqua. "Probing RNA structure in vivo." Current Opinion in Structural Biology 59 (December 2019): 151–58. http://dx.doi.org/10.1016/j.sbi.2019.07.008.

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McElroy, Gregory S., and Navdeep S. Chandel. "Probing mitochondrial metabolism in vivo." Proceedings of the National Academy of Sciences 116, no. 1 (December 18, 2018): 20–22. http://dx.doi.org/10.1073/pnas.1819614116.

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Wirtz, D., and B. R. Daniels. "Probing single cell micromechanics in vivo." Journal of Biomechanics 39 (January 2006): S588. http://dx.doi.org/10.1016/s0021-9290(06)85438-2.

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Burgess, Darren J. "Detailed probing of RNA structure in vivo." Nature Reviews Genetics 16, no. 5 (April 9, 2015): 255. http://dx.doi.org/10.1038/nrg3939.

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Sasse-Dwight, Selina, and Jay D. Gralla. "Probing co-operative DNA-binding in vivo." Journal of Molecular Biology 202, no. 1 (July 1988): 107–19. http://dx.doi.org/10.1016/0022-2836(88)90523-2.

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Xin, Li, Sun Yanan, and Wang Xiaochen. "Probing lysosomal activity in vivo." Biophysics Reports 7, no. 1 (2021): 1–7. http://dx.doi.org/10.52601/bpr.2021.200047.

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Valenta, T., M. Gay, S. Steiner, K. Draganova, M. Zemke, R. Hoffmans, P. Cinelli, M. Aguet, L. Sommer, and K. Basler. "Probing transcription-specific outputs of -catenin in vivo." Genes & Development 25, no. 24 (December 15, 2011): 2631–43. http://dx.doi.org/10.1101/gad.181289.111.

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McDonald, Beth M., Mateusz M. Wydro, Robert N. Lightowlers, and Jeremy H. Lakey. "Probing the orientation of yeast VDAC1 in vivo." FEBS Letters 583, no. 4 (January 29, 2009): 739–42. http://dx.doi.org/10.1016/j.febslet.2009.01.039.

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Radbruch, Helena, Daniel Bremer, Ronja Mothes, Robert Günther, Jan Rinnenthal, Julian Pohlan, Carolin Ulbricht, Anja Hauser, and Raluca Niesner. "Intravital FRET: Probing Cellular and Tissue Function in Vivo." International Journal of Molecular Sciences 16, no. 12 (May 21, 2015): 11713–27. http://dx.doi.org/10.3390/ijms160511713.

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Fujiwara, Masazumi, Simo Sun, Alexander Dohms, Yushi Nishimura, Ken Suto, Yuka Takezawa, Keisuke Oshimi, et al. "Real-time nanodiamond thermometry probing in vivo thermogenic responses." Science Advances 6, no. 37 (September 2020): eaba9636. http://dx.doi.org/10.1126/sciadv.aba9636.

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Real-time temperature monitoring inside living organisms provides a direct measure of their biological activities. However, it is challenging to reduce the size of biocompatible thermometers down to submicrometers, despite their potential applications for the thermal imaging of subtissue structures with single-cell resolution. Here, using quantum nanothermometers based on optically accessible electron spins in nanodiamonds, we demonstrate in vivo real-time temperature monitoring inside Caenorhabditis elegans worms. We developed a microscope system that integrates a quick-docking sample chamber, particle tracking, and an error correction filter for temperature monitoring of mobile nanodiamonds inside live adult worms with a precision of ±0.22°C. With this system, we determined temperature increases based on the worms’ thermogenic responses during the chemical stimuli of mitochondrial uncouplers. Our technique demonstrates the submicrometer localization of temperature information in living animals and direct identification of their pharmacological thermogenesis, which may allow for quantification of their biological activities based on temperature.
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Dissertations / Theses on the topic "In vivo probing"

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Scott, Nadia Aleyna. "Optical probing of hemodynamic responses in vivo with channelrhodopsin-2." Thesis, University of British Columbia, 2011. http://hdl.handle.net/2429/36449.

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Maintenance of neuronal function depends on the timely delivery of oxygen and glucose through changes in blood flow that are linked to the level of ongoing neuronal and glial activity, yet the mechanisms underlying this stimulus-dependent control of blood flow remain unclear. Here, using transgenic mice expressing channelrhodopsin-2 in a subset of layer 5b pyramidal neurons, we report that changes in intrinsic optical signals and blood flow can be evoked by activation of channelrhodopsin-2 neurons without direct involvement of other cell types. We have used a combination of imaging and pharmacology to examine the importance of glutamatergic synaptic signaling in neurovascular coupling. In contrast to sensory-evoked responses, we observed that glutamate-dependent neuronal signalling is not essential for the production of channelrhodopsin-evoked hemodynamic responses. Our results rather suggest that ChR2-activated neurons are coupled to the surrounding vasculature through a glutamate-dependent astrocytic pathway mediated by the Group I metabotropic glutamate receptor mGluR5.
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Lindell, Magnus. "Lead(II) as a Tool for Probing RNA Structure in vivo." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis: Univ.-bibl. [distributör], 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5780.

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Winters, Michael S. "Probing protein-protein interactions in vitro and in vivo with cyanogen." Cincinnati, Ohio : University of Cincinnati, 2002. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=ucin1027090541.

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Parent, Katherine L., and Katherine L. Parent. "Probing Neural Communication by Expanding In Vivo Electrochemical and Electrophysiological Measurements." Diss., The University of Arizona, 2017. http://hdl.handle.net/10150/626155.

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Neural communication is imperative for physical and mental health. Dysfunction in either ionic signaling or chemical neurotransmission can cause debilitating disorders. Thus, study of neurotransmission is critical not only to answer important fundamental questions regarding learning, decision making, and behavior but also to gain information that can provide insight into the neurochemistry of neurological disorders and lead to improved treatments. The work presented herein describes the development of techniques and instrumentation to enable advancements in neuroscientific inquiry. The effect of different temporal patterns and durations of simulation of the prefrontal cortex on dopamine release in the nucleus accumbens was examined and revealed a complex interaction that can help improve deep brain stimulation therapies. A measurement platform that combines electrophysiological and electrochemical techniques is described. The instrumentation is capable of concurrent monitoring of neural activity and dopamine release in vivo and in freely moving rodents. Analysis techniques to allow absolute quantification of tonic dopamine concentrations in vivo are detailed and the temporal resolution of the technique was vastly improved from ten minutes to forty seconds. An instrument that can simultaneously probe both dopamine and serotonin dynamics in either of their two temporal modes of signaling (tonic and phasic) using either fast-scan cyclic voltammetry or fast-scan controlled-adsorption voltammetry at two individually addressable microelectrodes is described. Together these new tools represent a significant step forward in the field of neuroanalytical chemistry by enable multiple brain regions, signaling modes (ionic flux in addition to both tonic and phasic neurotransmission), neurochemicals, and to be measured together.
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WINTERS, MICHAEL SHAWN. "PROBING PROTEIN-PROTEIN INTERACTIONS in vitro and in vivo WITH CYANOGEN." University of Cincinnati / OhioLINK, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1027090541.

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McCawley, Mark. "Comparing the efficacy of laser fluorescence and explorer examination in detecting subgingival calculus in vivo." Thesis, NSUWorks, 2015. https://nsuworks.nova.edu/hpd_cdm_stuetd/65.

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This paper investigated the sensitivity, specificity, accuracy, and precision of laser fluorescence and tactile probing for the detection of subgingival calculus. The gold standard for subgingival calculus detection has always been tactile probing. In this study 27 teeth were collected and 108 surfaces investigated, one tooth was excluded (group #13) where no calculus was observed on any surface, and three surfaces because of subgingival root caries to avoid confounding data, which left a total of 101 surfaces of 26 extracted teeth that meet the investigation criteria. The presence of subgingival calculus was observed on 75 tooth surfaces (74.25%). There was a correlation between tooth surface and the presence of calculus. Subgingival calculus was from most to least frequently observed on the Distal surface (92.0%), Lingual surface (76.9%), Mesial surface (70.8%) and Facial surface (57.7%). The amount of laser fluoresce increased according to the amount of subgingival calculus. There was a correlation between the amount of subgingival calculus and the amount of laser fluorescence. The tactile probing had a similar sensitivity compared to laser fluorescence for the detection of subgingival calculus. The laser fluorescence was more specific compared to tactile probing for the detection of subgingival calculus. The tactile probing had a similar accuracy compared to laser fluorescence for the detection of subgingival calculus. The laser fluorescence had more precision compared to tactile probing for the detection of subgingival calculus. These results show that by using both tactile probing and laser fluorescence the sensitivity, specificity, accuracy, and precision of detecting subgingival calculus can be increased. An increase in the sensitivity, specificity, accuracy, and precision of detecting subgingival calculus could help in the diagnosis and treatment of patients suffering from gingival recession and periodontal disease.
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Hong, Soyon Youngae. "Probing the In Vivo Economy of Amyloid Beta-Protein during the Development of Alzheimer's Disease-Type Pathology." Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10494.

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Despite intense therapeutic and diagnostic focus on dyshomeostasis of amyloid \(\beta\)-peptide \((A\beta)\) in Alzheimer’s disease (AD), we still lack insight into the in vivo economy of \(A\beta\) in the normal and diseased brain. Thus, my thesis research focused on understanding the dynamics of \(A\beta\) in the living brain during the development of AD-type pathology. Using in vivo microdialysis, I showed that the steady-state level of \(A\beta\) that remains diffusible in the hippocampal interstitial fluid (ISF) of awake, behaving hAPP transgenic mice falls as \(A\beta\) steadily accumulates in the brain parenchyma. In accord, I observed distinct dispositions of microinjected radiolabeled \(A\beta\) in plaque-rich versus plaque-free mice, suggesting that cerebral amyloid deposits rapidly sequester newly released \(A\beta\). This provides the first in vivo evidence from controlled animal experiments for the hypothesis that soluble \(A\beta42\) in human cerebrospinal fluid (CSF) falls in AD because it is sequestered into insoluble parenchymal deposits as the disease develops. My data further show that the association of \(A\beta\) with insoluble parenchymal deposits is not irreversible, as acute inhibition of \(\gamma\)-secretase in plaque-rich mice failed to lower ISF \(A\beta42\), whereas it did in plaque-free mice. Hence, the ISF in plaque-rich mice seems to be a reservoir for both newly produced \(A\beta\) and \(A\beta\) that diffuse off of cell membrane- and plaque-bound deposits. Finally, I showed that \(A\beta\) dimers, which are known to be potent synaptic neurotoxins, are undetectable in the aqueous compartments of the central nervous system, i.e., the brain ISF and CSF, in hAPP transgenic mice. Acute injection of \(A\beta\) dimers into living wild-type mice showed a rapid sequestration of the dimers away from the hippocampal ISF pool and a higher recovery in the membrane-bound pool than in the cytosolic pool of the brain homogenates. Interestingly, I found that the \(A\beta\) recovered in the membrane-bound pool was tightly associated with endogenous GM1 ganglioside. Taken together, my results suggest that \(A\beta\) dimers, and probably higher oligomers, are rapidly sequestered away from the ISF and bind to GM1 ganglioside-enriched lipid membranes, such as raft-like microdomains of secreted vesicles or on the plasma membranes of neurons and other cells.
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Ajuh, Paul Munya. "28S ribosomal RNA in Xenopus : gene sequence analysis and secondary structure probing of in vitro and in vivo transcripts." Thesis, University of Liverpool, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.304878.

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Jansen, Valerie Malyvanh. "Generation and characterization of a knock-in allele of EKLF probing the in vivo role of the chromatin remodeling domain in definitive hematopoietic cells /." View the abstract View the abstract Download the full-text PDF version Download the full-text PDF version, 2009. http://etd.uthsc.edu/ABSTRACTS/2009-025-Jansen-index.htm.

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Thesis (Ph.D.)--University of Tennessee Health Science Center, 2009.
Title from title page screen (viewed on February 4, 2010). Research advisor: John M. Cunningham, M.D. Document formatted into pages (xiv, 115 p. : ill.). Vita. Abstract. Includes bibliographical references (p. 89-103).
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Patel, Ankit Rajnikant. "Probing tethered vesicle assemblies using quartz crystal microbalance with dissipation monitoring : antibody binding and other applications towards ex vivo, label-free membrane protein analysis /." May be available electronically:, 2007. http://proquest.umi.com/login?COPT=REJTPTU1MTUmSU5UPTAmVkVSPTI=&clientId=12498.

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Book chapters on the topic "In vivo probing"

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Kouzine, Fedor, Damian Wojtowicz, Arito Yamane, Rafael Casellas, Teresa M. Przytycka, and David L. Levens. "In Vivo Chemical Probing for G-Quadruplex Formation." In Methods in Molecular Biology, 369–82. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9666-7_23.

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Ritchey, Laura E., Zhao Su, Sarah M. Assmann, and Philip C. Bevilacqua. "In Vivo Genome-Wide RNA Structure Probing with Structure-seq." In Methods in Molecular Biology, 305–41. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9045-0_20.

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Varol, Chen, Limor Landsman, and Steffen Jung. "Probing In Vivo Origins of Mononuclear Phagocytes by Conditional Ablation and Reconstitution." In Macrophages and Dendritic Cells, 71–87. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-396-7_6.

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Chamiolo, Jasmine, Imre Gaspar, Anne Ephrussi, and Oliver Seitz. "In Vivo Visualization and Function Probing of Transport mRNPs Using Injected FIT Probes." In Methods in Molecular Biology, 273–87. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7213-5_18.

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Mudumbi, Krishna C., and Weidong Yang. "Probing Protein Distribution Along the Nuclear Envelope In Vivo by Using Single-Point FRAP." In Methods in Molecular Biology, 113–22. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3530-7_6.

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Pemmari, Toini, Tiia Koho, and Tero A. H. Järvinen. "Probing Vasculature by In Vivo Phage Display for Target Organ-Specific Delivery in Regenerative Medicine." In Vascularization for Tissue Engineering and Regenerative Medicine, 1–26. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-319-21056-8_21-1.

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Muller, Florian L., James Mele, Holly Van Remmen, and Arlan Richardson. "Probing the in Vivo Relevance of Oxidative Stress in Aging Using Knockout and Transgenic Mice." In Aging at the Molecular Level, 131–44. Dordrecht: Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-0667-4_10.

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Tomezsko, Phillip, Harish Swaminathan, and Silvi Rouskin. "DMS-MaPseq for Genome-Wide or Targeted RNA Structure Probing In Vitro and In Vivo." In Methods in Molecular Biology, 219–38. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-1158-6_13.

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Pemmari, Toini, Tiia Koho, and Tero A. H. Järvinen. "Probing Vasculature by In Vivo Phage Display for Target Organ-Specific Delivery in Regenerative Medicine." In Vascularization for Tissue Engineering and Regenerative Medicine, 179–204. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-319-54586-8_21.

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Rather, Irfan A., Vivek K. Bajpai, and Yong-Ha Park. "In Vitro and In Vivo Inhibition of Atopic Dermatitis (AD) by a Novel Probiotic Isolate Lactobacillus sakei Probio-65." In Microbiology Monographs, 19–38. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-23213-3_2.

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Conference papers on the topic "In vivo probing"

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Fujiwara, Masazumi, Simo Sun, Alexander Dohms, Yushi Nishimura, Ken Suto, Yuka Takezawa, Keisuke Oshimi, et al. "Real-time nanodiamond thermometry probing in vivo thermogenic responses." In Optical and Quantum Sensing and Precision Metrology, edited by Selim M. Shahriar and Jacob Scheuer. SPIE, 2021. http://dx.doi.org/10.1117/12.2582685.

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Ashkenazi, S., S. W. Huang, T. Horvath, Y. E. L. Koo, and R. Kopelman. "Oxygen sensing for in vivo imaging by photoacoustic lifetime probing." In Biomedical Optics (BiOS) 2008, edited by Alexander A. Oraevsky and Lihong V. Wang. SPIE, 2008. http://dx.doi.org/10.1117/12.764268.

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Kim, Daeun, Jessica L. Wisnowski, Christopher T. Nguyen, and Justin P. Haldar. "Probing in vivo microstructure with T1-T2 relaxation correlation spectroscopic imaging." In 2018 IEEE 15th International Symposium on Biomedical Imaging (ISBI 2018). IEEE, 2018. http://dx.doi.org/10.1109/isbi.2018.8363664.

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Thueler, Philippe, Michel St Ghislain, Christian Depeursinge, Igor Charvet, Paolo Meda, and Ben Vermeulen. "Optical local and superficial probing of tissues for in vivo diagnosis." In European Conference on Biomedical Optics. Washington, D.C.: OSA, 2003. http://dx.doi.org/10.1364/ecbo.2003.5141_266.

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Lister, K., Zhan Gao, and J. P. Desai. "Real-time, haptics-enabled simulator for probing ex vivo liver tissue." In 2009 Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 2009. http://dx.doi.org/10.1109/iembs.2009.5333410.

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Thueler, Philippe, Michel St. Ghislain, Christian D. Depeursinge, Igor Charvet, Paolo Meda, and Ben Vermeulen. "Optical local and superficial probing of tissues for in vivo diagnosis." In European Conference on Biomedical Optics 2003, edited by Georges A. Wagnieres. SPIE, 2003. http://dx.doi.org/10.1117/12.500831.

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Colliaux, David, Lia Giraud, Claude Yéprémian, Pierre Bessière, and Jacques Droulez. "Probing models of minimal swimming vehicules in vivo with microalgae phototaxis." In European Conference on Artificial Life 2015. The MIT Press, 2015. http://dx.doi.org/10.7551/978-0-262-33027-5-ch079.

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Duric, Nebojsa, Peter Littrup, Erik West, Bryan Ranger, Cuiping Li, and Steven Schmidt. "In-vivo imaging of breast cancer with ultrasound tomography: probing the tumor environment." In SPIE Medical Imaging, edited by Jan D'hooge and Marvin M. Doyley. SPIE, 2011. http://dx.doi.org/10.1117/12.878939.

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Yendiki, Anastasia. "Challenges in MR image acquisition and analysis for probing the human connectome in vivo." In 2010 7th IEEE International Symposium on Biomedical Imaging: From Nano to Macro. IEEE, 2010. http://dx.doi.org/10.1109/isbi.2010.5490098.

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Rauzi, Matteo, Eric P. Mottay, Thomas Lecuit, and Pierre-François Lenne. "Probing the mechanical properties of Drosophila embryo ephitelial cells in vivo by laser nanodissection." In SPIE BiOS: Biomedical Optics, edited by Daniel L. Farkas, Dan V. Nicolau, and Robert C. Leif. SPIE, 2009. http://dx.doi.org/10.1117/12.808171.

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