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Journal articles on the topic 'In vitro'

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Author: Grafiati

Published: 4 June 2021

Last updated: 29 July 2024

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1

Condori, Rosa, Carlos Quispe, Edith Ancco, Deysi Dipaz, and Edwin Mellisho. "SOBREVIVENCIA DE BLASTOCISTOS BOVINOS PRODUCIDOS IN VITRO VITRIFICADOS EN DISPOSITIVOS VITRI-TIP Y VITRI-TOP." SPERMOVA 9, no. 1 (August 31, 2019): 48–52. http://dx.doi.org/10.18548/aspe/0007.07.

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2

AKIMOVA, S. V., V. V. KIRKACH, A. K. RADZHABOV, and G. E. TER-PETROSYANTS. "MORPHOBIOLOGICAL FEATURES OF DIAPHRAGM FORMATION IN IN VITRO AND EX VITRO GRAPE PLANTS OF INTERSPECIFIC ORIGIN." Izvestiâ Timirâzevskoj selʹskohozâjstvennoj akademii, no. 6 (2021): 5–13. http://dx.doi.org/10.26897/0021-342x-2021-6-5-13.

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Clonal micropropagation of grape varieties of interspecific origin Moscow white (Vitis amurensis Rupr. × Vitis vinifera L.) showed that on the 40th day of the animation stage, a monopodial type of branching is typical for micro-plants, and there is no diaphragm in all nodes of micro-shoots. On the 120th day of growing in containers, the researchers revealed that the nodes of the plant shoots, from the 1st to the 6th, have a monopodial branching type. Parenchymal cells which form an incomplete diaphragm are present in the seventh node. A fully formed diaphragm appears from the eighth node, and the branching type switches to a sympodial-monopodial one.

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3

Teixeira da Silva, J. A. "CHRYSANTHEMUM: EX VITRO TO IN VITRO TO EX VITRO." Acta Horticulturae, no. 616 (November 2003): 443–47. http://dx.doi.org/10.17660/actahortic.2003.616.68.

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4

Mikhailova, Mikhailova N. D., Mishieva N. G. Mishieva, Kirillova A. O. Kirillova, Martazanova B. A. Martazanova B, and Dzhincharade L. G. Dzhincharade. "In vitro oocyte maturation." Akusherstvo i ginekologiia 11_2021 (November 26, 2021): 64–70. http://dx.doi.org/10.18565/aig.2021.11.64-70.

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5

Apóstolo, Nancy M., Ezequiel E. Larraburu, Miriam N. Gil, María A. Zapater, and Berta E. Llorente. "In vitro and ex vitro germination of three Handroanthus species (Bignoniaceae)." Bonplandia 25, no. 1 (January 1, 2016): 5. http://dx.doi.org/10.30972/bon.2511267.

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Handroanthus impetiginosus, H. lapacho and “H.ochraceuslapachos” se distribuyen en el NO Argentino y presentan inconvenientes de germinación y conservación en su ambiente natural. La germinación de semillas bajo condiciones controladas es una alternativa para asegurar la propagación de especies con este tipo de problemáticas. En el presente estudio integral, se analizó la germinación in vitroy ex vitro, las características de las semillas y la morfología de las plántulas de las tres especies de Handroanthusmencionadas. Para ello, se midió el largo y ancho de las semillas, el ancho de las alas de la cubierta seminal, el ancho y largo del cuerpo seminal y del embrión. El poder germinativo de las tres especies fue determinado durante 12 meses luego de la cosecha de las semillas. Fueron determinados los parámetros de las plántulas obtenidas in vitroy ex vitro. El tamaño de la semilla y embrión de H. impetiginosus.

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6

Zinatullina, A. E., and V. I. Nikonov. "LABORATORY EVALUATION OF REGENERATES OF WHEAT HYBRID COMBINATIONS IN VITRO AND EX VITRO CONDITIONS." ÈKOBIOTEH 4, no. 2 (2021): 81–88. http://dx.doi.org/10.31163/2618-964x-2021-4-2-81-88.

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Drought is the combination of climatic conditions that leads to a long-term shortage of water in the soil and air. This is one of the most common abiotic stress factors that leads to significant losses of crop yield and the emergence of a threat to food security. Researchers are actively developing ways to create drought-resistant zoned varieties of economically important agricultural crops and especially cereals as the main food resource. Such varieties should maintain a relatively high yield rate with a shortage of water in the soil and air. The aim of the work was the laboratory evaluation in vitro and ex vitro of wheat regenerants formed in the embryo culture in vitro under conditions selective for the indicator "drought resistance". Methods of embryo culture in vitro, laboratory evaluation of caryopsis viability, histological analysis, as well as statistical processing of the received results were used. Under the conditions of in vitro experiments on the selective medium simulating drought by introducing mannit at the concentration of 8% as an osmotic, regenerants of 5 hybrid wheat combinations that showed tolerance to stress were obtained. It is shown that the development of regenerants in vitro and ex vitro pass according to the same phenological phases and in the same duration as donor plants. Regenerants form caryopsises of sufficiently high quality, which is confirmed by laboratory observations of their viability and histological analysis of seedlings.

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7

Chakravarthy, U., D. McCormick, C. J. F. Maguire, and D. B. Archer. "An in-vitro study of irradiated vitreo-retinal membranes." Eye 1, no. 1 (January 1987): 126–35. http://dx.doi.org/10.1038/eye.1987.19.

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8

Harmanchuk, LV, OM Makarenko, NM Khranovs'ka, TV Nikolaienko, VV Nikulina, KhD Nepyĭvoda, LI Ostapchenko, SH Morozov, and MS Kositsyn. "Mitokorrektine stimulates angiogenesis in vitro." Fiziolohichnyĭ zhurnal 59, no. 2 (May 15, 2013): 52–58. http://dx.doi.org/10.15407/fz59.02.052.

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9

Babanina, Svetlana, Natalya Egorova, Olga Yakimova, and Mariya Kovalenko. "ADAPTATION TO EX VITRO CONDITIONS OF MICROPLANTS LAVANDULA ANGUSTIFOLIA MILL. AT LONG-TERM REPRODUCTION IN VITRO." Vestnik of Kazan State Agrarian University 18, no. 3 (October 2, 2023): 11–19. http://dx.doi.org/10.12737/2073-0462-2023-11-19.

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The purpose of the study is to identify the features of adaptation to ex vitro conditions of lavender plants after long-term clonal micropropagation. The experiments were carried out on microplants of narrow-leaved lavender (Lavandula angustifolia Mill.), cv. The number of plants in each subcultivation - n=10 pcs., 3-fold repetition. Microplants with well-developed shoots and roots were planted in a mixture of peat and perlite (1:1) and grown at illumination of 2–3 klx, photoperiod duration of 16 h, temperature of 24 ± 2°C, air humidity of 70%. The frequency of adaptation of microplants, depending on the number of subcultivations, varied slightly and amounted to 83...100%. On the 60th day of adaptation, the length of the shoot was significantly higher by 21...28% in microplants after 8 subcultivations (206.73 mm) compared with 14, 15 and 16 passages. There were no differences in the length of additional shoots depending on the amount of subculturing. According to the number of nodes on the main shoot, a tendency to their decrease with an increase in the number of passages was observed. A significant increase in the content of chlorophyll a with an increase in the number of subcultivations on the 14th day of adaptation was revealed, however, later these differences leveled out. On average, the in vitro viability index for passages was 1.45 and increased up to 30 days of adaptation to 1.75. The revealed features of changes in morphometric and physiological parameters indicate a good adaptive ability of the analyzed plants, while micropropagation in vitro during 16 subcultivations did not significantly reduce their adaptive potential. The optimal period of ex vitro adaptation is the period of 45...60 days, during which the plants formed well-developed shoots (3.20...6.00 g) and root system (0.619...1.143 g).

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10

Yurin, D. V., V. V. Nevzorova, A. A. Balbutskaya, and S. S. Belimova. "Antimicrobial activity of enrofloxacin in vitro." International bulletin of Veterinary Medicine 2 (2020): 99–103. http://dx.doi.org/10.17238/issn2072-2419.2020.2.99.

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Continuouse use of enrofloxacin contributes to emergence of enrofloxacin-resistant mi-crobial resistance, isolated and reported late-ly. In this study we deal with the spread of resistance of enrofloxacin among pathogenic organisms, infecting animals. The suscepti-bility to enrofloxacin was studied in standard disc diffusion assay. We studied 437 bacteri-al isolates in total. Salmonella dublin and Sal-monella typhimurium showed the highest suscepti-bility to enrofloxacin (100%); Salmonella enter-itidis and Salmonella choleraesuis proved a bit less susceptibility (95% and 94,7%). 5% of S. enter-itidis isolates and 5.3% of S. choleraesuis isolates had intermediate susceptibility. We did not register any resistance of isolates of Salmonella, Pasteurella and Morganella (Pasteurella multocida, Morganel-la morganii). 83.9% of Escherichiacoli strains proved susceptibility to enrofloxacin, the zone of retardation in 6.4% of the isolates was in corre-spondence with intermediate susceptibility, 9.7% of the isolates proved to be resistant. 90,9% of Pseudomonas aeruginosa isolates in our study was susceptible to enrofloxacin, 9.1% of them had intermediate susceptibility. The isolates of Strepto-coccus spp. and Staphylococcus pseudintermedius re-vealed high susceptibility to enrofloxacin, also as Listeria monocytogenes (causative agent of listeriosis)and Erysipe-lothrix rhusiopathiae (causative agent of swine erysipelas). 87.5% of the coagulase negative staphylococci proved susceptible to enrofloxacin; 6.25% of the isolates were resistant or had intermediate susceptibility. The shares of susceptible isolates of Staphylococcus hyicus, Staphylococ-cus aureus and Streptococcus uberis were respec-tively 65.1%, 75%, 75%. The shares of isolates with intermediate susceptibility of the same spp. were respectively 9.3%, 15%, 25%. The shares of resistant isolates of Staphylococci were respective-ly 25.6% and 10%. We found no strains of Str. uberis with resistance to enrofloxacin. As for Enterococci, 52.4% of the isolates were enrofloxacin-susceptible, 11,9% and 37,7% of them were re-spectively enrofloxacin-resistant or had intermedi-ate susceptibility. Presently most Gram-negative pathogenic bacteria have no resistance to enroflox-acin. Notwithstanding that enrofloxacin is signifi-cantly less effective against such pathogenic organ-isms as Staphylococci and Streptococci.

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11

ERKEKOĞLU, Pınar, and Belma KOÇER GÜMÜŞEL. "In Vitro Liver Models in Toxicology." Journal of Literature Pharmacy Sciences 8, no. 1 (2019): 1–17. http://dx.doi.org/10.5336/pharmsci.2018-61664.

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12

Дружинина, Юлия, and Yuliya Druzhinina. "Legal Regime of Embryo in Vitro." Journal of Russian Law 5, no. 12 (December 19, 2017): 129–41. http://dx.doi.org/10.12737/article_5a200506899599.19842755.

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13

ДЕШКО, А., Л. ГОЛУБЕЦ, and В. СЕМЕНОВ. "Producing cattle embryos in vitro." Животноводство России, no. 5 (May 27, 2024): 33–36. http://dx.doi.org/10.25701/zzr.2024.06.006.

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Будучи длительным, высокотехнологичным и достаточно сложным комплексным процессом, получение эмбрионов in vitro требует понимания метаболических потребностей гамет и эмбрионов. В статье приведены результаты опыта по получению эмбрионов in vitro от коров голштинской породы.

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14

Lubell-Brand, Jessica D., Lauren E. Kurtz, and Mark H. Brand. "An In Vitro–Ex Vitro Micropropagation System for Hemp." HortTechnology 31, no. 2 (April 2021): 199–207. http://dx.doi.org/10.21273/horttech04779-20.

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Hyperhydricity of shoots initiated in vitro, poor shoot extension, inability of shoot cultures to maintain good growth over an extended time, and unsuccessful ex vitro rooting have limited the development of a commercial scale micropropagation system for hemp (Cannabis sativa). We present a culture initiation method that prevents shoot hyperhydricity using vented-lid vessels with 0.2-µm pores and medium containing agar at 1% (w/v). To optimize shoot multiplication in vitro, a control medium (medium A) and four treatment media (medium B, C, D, and E), with varying inorganic nutrients and vitamins were tested. Control medium A consisted of 1× Murashige and Skoog (MS) with vitamins plus 3% (w/v) sucrose, 0.5 mg·L−1 metatopolin, 0.1 mg·L−1 gibberellic acid, and 0.8% agar (w/v) at pH 5.7. The four treatment media differed from the control medium as follows: medium B, 2.5× MS with vitamins; medium C, 1× MS with vitamins plus added mesos [calcium chloride (anhydrous), magnesium sulfate (anhydrous), and potassium phosphate (monobasic) nutrients]; medium D, 1× MS with vitamins plus added vitamins; and medium E, 1× MS with vitamins plus added mesos and vitamins. Medium C and medium E produced more microcuttings than the control at 6 weeks after the initial subculture with shoot multiplication media and all other treatments at 9 and 12 weeks. Shoots grown on these two media displayed optimal extension and leaf lamina development; however, they exhibited slight chlorosis by 12 weeks after subculture with shoot multiplication media. In a separate experiment, medium E was supplemented with ammonium nitrate at 0, 500, 1000, or 1500 mg·L−1, and cultures grown with 500 mg·L−1 produced the most microcuttings and exhibited the best combination of shoot extension and leaf lamina development. We provide a method of prerooting microshoots in vitro that has resulted in 75% to 100% rooting ex vitro in rockwool. Using 10 recently micropropagated plants, ≈300 retip cuttings (cuttings taken from new shoots from recently micropropagated plants) were harvested over 10 weeks. The average weekly rooting was more than 90%. Retipping can produce nine-times as many plants in a similar amount of floor space as stem cuttings derived from traditional stock mother plants. The micropropagation/retipping method proposed can be a more efficient way to generate clonal liner plants for commercial-scale production.

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15

Olszanska, B., and U. Stepinska. "In vitro fertilization of in vitro ovulated quail oocytes." British Poultry Science 41, sup001 (September 2000): 22–23. http://dx.doi.org/10.1080/00071660050148598.

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16

Lu, K. H., I. Gordon, M. P. Boland, and T. F. Crosby. "In Vitro Fertilization of Bovine Oocytes Matured In Vitro." Proceedings of the British Society of Animal Production (1972) 1987 (March 1997): 28. http://dx.doi.org/10.1017/s0308229600034693.

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The development of an efficient laboratory procedure which would enable cattle ovarian oocytes to be matured in vitro, fertilized and cultured in vitro to the blastocyst stage of development could have important practical and scientific implications. The commercial exploitation of certain embryo transfer techniques applicable in cattle (eg., twinning by embryo transfer) might be facilitated by the development of such a procedure and there would be many advantages to having a cheap source of embryos available for research purposes. The present report deals with some of the studies recently carried out in this laboratory aimed at utilising follicular oocytes recovered from the ovaries of cattle slaughtered for beef at the abattoir. Such studies have been undertaken over a period of almost twenty years, starting with the work of Sreenan (1968)* but it now realised that the oocytes of farm mammals are incapable of normal development until after the completion of complex changes during maturation.

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17

Del Campo, M. R., M. X. Donoso, J. J. Parrish, and O. J. Ginther. "In vitro fertilization of in vitro-matured equine oocytes." Journal of Equine Veterinary Science 10, no. 1 (January 1990): 18–22. http://dx.doi.org/10.1016/s0737-0806(06)80078-x.

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18

Lu, K. H., M. P. Boland, T. F. Crosby, and I. Gordon. "In vitro fertilization of cattle oocytes matured in vitro." Theriogenology 27, no. 1 (January 1987): 251. http://dx.doi.org/10.1016/0093-691x(87)90128-2.

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19

Singh, Gurpreet, S. M. Totey, and G. P. Talwar. "In vitro fertilization of buffalo () oocytes matured in vitro." Theriogenology 31, no. 1 (January 1989): 255. http://dx.doi.org/10.1016/0093-691x(89)90663-8.

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20

Wu, G. M., P. C. Qin, J. H. Tan, and L. A. Wang. "In vitro fertilization of in vitro matured pig oocytes." Theriogenology 37, no. 1 (January 1992): 323. http://dx.doi.org/10.1016/0093-691x(92)90392-5.

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21

Betancourt, M., R. Fierro, and D. Ambriz. "In vitro fertilization of pig oocytes matured in vitro." Theriogenology 40, no. 6 (December 1993): 1155–60. http://dx.doi.org/10.1016/0093-691x(93)90286-e.

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22

Tůmová, L., J. Řimáková, J. Tůma, and J. Dušek. "Silybum marianum in vitro-flavonolignan production." Plant, Soil and Environment 52, No. 10 (November 17, 2011): 454–58. http://dx.doi.org/10.17221/3466-pse.

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The effect of coniferyl alcohol as a precursor of flavonolignan biosynthesis on silymarin components production in Silybum marianum suspension culture was studied. Coniferyl alcohol showed the changes in silymarin complex production. Silydianin was detected mainly in the control samples of cultivated cells. A significant increase of silydianin was observed only after 72 h of the application of 46µM coniferyl alcohol. No other components of the silymarin complex (silychristin and silybin) were detected; neither in control samples nor after the precursor feeding. But the increased accumulation of taxifolin (flavanole) was very interesting. The highest taxifolin production was reached after 48 hours of treatment(about 554% compared to control).

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23

Petrová, I., M. Sedmíková, E. Chmelíková, D. Švestková, and R. Rajmon. "In vitro aging of porcine oocytes." Czech Journal of Animal Science 49, No. 3 (December 12, 2011): 93–98. http://dx.doi.org/10.17221/4285-cjas.

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Porcine oocytes matured in vitro develop in various ways if they are further cultivated. In our studies these oocytes were cultivated for 1 to 5 days (in vitro aging). During the 1st day of aging, most of them remained at the stage of metaphase II (98%). Then many oocytes underwent the spontaneous parthenogenetic activation. The portion of activated oocytes reached its peak after 2 or 3 days of aging in vitro (39 or 45%). The portion of fragmented oocytes peaked at the same time (28%). During subsequent aging in vitro (i.e. day 4 or 5 of aging), the portion of lysed oocytes significantly increased (30 or 37%). The highest portion of spontaneously activated parthenogenetic embryos at a pronuclear stage (35%) was observed during the 2nd day of aging in vitro. These pronuclear embryos had mainly one polar body with two pronuclei (47% of all pronuclear embryos) or two polar bodies with one pronucleus (38% of all pronuclear embryos). During the 3rd and 5th day of in vitro aging, there was a significant increase in the portion of parthenogenetic embryos cleaved to the 2-cell or 3-cell stage. When considering the prolonged in vitro culture of porcine oocyte, only the first day of aging should be taken into account, since beyond this time significant changes, i.e. parthenogenesis, fragmentation or lysis, occurred in oocytes under in vitro conditions.  

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24

Sorochinsky, B. V., and A. I. Prohnevsky. "D2O inhibit microtubules assembly in vitro." Biopolymers and Cell 7, no. 3 (May 20, 1991): 95–98. http://dx.doi.org/10.7124/bc.0002d7.

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25

Piven, N. M., G. G. Melnichuk, and A. S. Felaliev. "In vitro induced morphogenesis of walnut." Biopolymers and Cell 7, no. 4 (July 20, 1991): 61–65. http://dx.doi.org/10.7124/bc.0002e2.

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26

Espinosa Reyes, Angel Luis, Eugenio Torres Rodriguez, and Juan Jose Silva Pupo. "In vitro propagation of Anredera vesicaria." Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas 23, no. 5 (September 30, 2024): 732–39. http://dx.doi.org/10.37360/blacpma.24.23.5.45.

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Anredera vesicariais a plant whit high anti-inflammatory activity. The work objective was to establish in vitropropagation of A. vesicaria. Nodal segments were used as explants and two disinfection times in 1% sodium hypochlorite (15 and 20 minutes) were evaluated during in vitroestablishment. Combinations of AG3 (2,5 and 5,0 mg/L) and IAA (0,05 and 0,1 mg/L) were evaluated in the multiplication phase and the effect of IBA (0,5 and 1,0 mg /L) and Pectimorf (1,0 and 5,0 mg/L) for in vitrorooting. Acclimatization was carried out in a mixture of soil-cow manure-zeolite. Disinfection was achieved with 1% sodium hypochlorite for 15 minutes and in vitroestablishment in the MS (1962) culture medium. High values of multiplication and rooting in vitrowere obtained, as well as acclimatization of plants in vitro.

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27

Funahashi, Hiroaki, Thomas C. Cantley, Todd T. Stumpf, Steven L. Terlouw, and Billy N. Day. "In Vitro Development of in Vitro-Matured Porcine Oocytes Following Chemical Activation or in Vitro Fertilization1." Biology of Reproduction 50, no. 5 (May 1, 1994): 1072–77. http://dx.doi.org/10.1095/biolreprod50.5.1072.

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28

Kirdmanee, C., Y. Kitaya, and T. Kozai. "Effects of CO2 enrichment and supporting materialin vitro on photoautotrophic growth ofEucalyptus plantletsin vitro andex vitro." In Vitro Cellular & Developmental Biology - Plant 31, no. 3 (July 1995): 144–49. http://dx.doi.org/10.1007/bf02632010.

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29

Маyorova, О. Yu. "ADAPTATION OF THE OBTAINED in vitro Gentiana lutea L. PLANTS TO ex vitro AND in situ CONDITIONS." Biotechnologia Acta 8, no. 6 (2015): 77–86. http://dx.doi.org/10.15407/biotech8.06.077.

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30

Ilyushko, M. V., and M. V. Romashova. "RICE TETRAPLOID FORMATION IN ANDROGENESIS in vitro." Rossiiskaia selskokhoziaistvennaia nauka, no. 3 (2020): 14–17. http://dx.doi.org/10.31857/s2500262720030047.

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31

Buev, D. O., A. M. Emelin, I. A. Yakovlev, and R. V. Deev. "CULTIVATION OF MYOBLASTS AND MYOSATELLITOCYTES IN VITRO." NAUKA MOLODYKH (Eruditio Juvenium) 8, no. 1 (March 31, 2020): 86–97. http://dx.doi.org/10.23888/hmj20208186-97.

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Sidekhmenova, A. V., O. I. Aliev, N. S. Domnina, P. S. Vlasov, E. V. Popova, and M. B. Plotnikov. "A new in vitro blood hyperviscosity model." Bulletin of Experimental Biology and Medicine 172, no. 10 (2021): 525–28. http://dx.doi.org/10.47056/0365-9615-2021-172-10-525-528.

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33

Povnitsa, O. Yu, L. O. Biliavska, Yu B. Pankivska, K. S. Naumenko, L. B. Zelena, S. D. Zagorodnya, and V. P. Atamanyuk. "Anti-Adenoviral Activity of Neoflazid in vitro." Mikrobiolohichnyi Zhurnal 80, no. 5 (September 30, 2018): 98–109. http://dx.doi.org/10.15407/microbiolj80.05.098.

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34

Zholudenko, Y., and N. Zholobak. "ANTIHERPETIC ACTION OF CERIUM SALTS IN VITRO." Bulletin of Taras Shevchenko National University of Kyiv. Series: Biology 89, no. 2 (2022): 28–31. http://dx.doi.org/10.17721/1728.2748.2022.89.28-31.

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Compounds based on cerium are highly promising objects in biotechnology regarding their high biological activities such as antiviral, antibacterial, antifungal, neuro- and radioprotective action, and antioxidant activity. On their basis is possible to develop compositions capable of activating the systems of cellular and humoral immune defence and use them for the prevention and therapy of viral diseases, which makes it achievable to use them for the development of potential antiherpetic agents. Despite the success of their application in biotechnological fields, the mechanism of their action on biological objects requires detailed research. The work aimed to verify in vitro anti-HSV-1/2 activity of trivalent and tetravalent cerium salts (1 mM – 0.01 nM) according to the preventive and therapeutic regimen. Methods: virological, cytological, statistical. Results. The therapeutic regime was noneffective. In the preventive regime, salt (NH4)2Ce(NO3)6 in vitro forms antiviral resistance in the range of investigated concentrations, while the salt CeCl3·7Н2О forms a non-linear, sinusoidal-like concentration-dependent anti-HSV-1/2 response of cells. Conclusions. Cerium salts (III and IV) can cause the formation of a state of antiviral resistance in the model system MA-104 - HSV-1/2 during their previous 24 h of contact with test cells. Cerium salt (IV) provides 50% inhibition of the cytopathic action of HSV-1/2 at a concentration of 1 μM. It is assumed that the shown antiviral activity of cerium salts may be due to their effect on the interferon system and the formation of antiviral resistance in cells.

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35

Puzyrnova, Valentina, and Natalya Doroshenko. "EFFICIENCY OF CULTIVATION OF GRAPEVINE IN VITRO*." Vestnik of Kazan State Agrarian University 17, no. 4 (January 27, 2023): 39–44. http://dx.doi.org/10.12737/2073-0462-2023-39-44.

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The paper is devoted to improving the elements of the technology of clonal micropropagation of Pukhlyakovsky grapevine variety in order to increase the efficiency of its content in the in vitro collection. The introduction of an osmotically active substance ‒ sorbitol into the culture medium in the concentration range of 5-30 g/l in comparison with sucrose of 10 g/l was studied. The research was carried out in 2021-2022 on the collection of grapevine plants in vitro of the All-Russian Research Institute for Viticulture and Winemaking named after Ya.I. Potapenko in Novocherkassk (Rostov region). Cultivation was carried out in conditions of illumination of 3.0 thousand lux, temperature +25 ° C, photoperiod of 16/8 hours, air humidity 60 %. Morphometric parameters of shoots were evaluated: the number and length of shoots, shoot height, number of leaves, inhibition of growth processes was noted in all experimental variants in comparison with the control. The maximum safety indicators were recorded in the variant with a sorbitol concentration of 10 g/l. The minimum safety was observed in the control (43.3 %) and the variant with the maximum sorbitol content ‒ 30 g/l (36.7 %). The calculation of the economic efficiency of keeping plants in the collection in vitro on a medium with sucrose and sorbitol was carried out. Depositing on a medium with sorbitol is 17 % more economical due to an increase in the interval between subcultivations from 6 months to 12. The positive effect of the drug sorbitol on the safety and quality characteristics of plants was established, the maximum safety (86.7 %) and moderate inhibition of growth were recorded in the variant with a concentration of the drug 10 g/l. We consider this concentration to be optimal for use when depositing Puhlyakovsky grape plants in the in vitro collection under conditions of slow growth.

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36

Sublon, Roland. "Education in vitro." Revue des Sciences Religieuses 61, no. 4 (1987): 198–208. http://dx.doi.org/10.3406/rscir.1987.3078.

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Jennings, Jenifer C., Kimberly Moreland, and C. Matthew Peterson. "In Vitro Fertilisation." Drugs 52, no. 3 (September 1996): 313–43. http://dx.doi.org/10.2165/00003495-199652030-00002.

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38

Renz, H., W. M. Becker, A. Bufe, J. K. Kleine-Tebbe, M. Raulf-Heimsoth, J. Saloga, Th Werfel, and M. Worm. "In-vitro-Allergiediagnostik." Allergologie 26, no. 06 (June 1, 2003): 237–54. http://dx.doi.org/10.5414/alp26237.

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39

Skevaki, C. L., and H. Renz. "In-vitro-Allergiediagnostik." Allergologie 37, no. 10 (October 1, 2014): 403–11. http://dx.doi.org/10.5414/alx01687.

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40

Iwasawa, Kentaro, and Takanori Takebe. "Organogenesis in vitro." Current Opinion in Cell Biology 73 (December 2021): 84–91. http://dx.doi.org/10.1016/j.ceb.2021.06.007.

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41

M, Abid, Rupali ., G. Islam, K. Gahlot, and NA Khan. "IN VITRO FERTILIZATION." Journal of Biological & Scientific Opinion 1, no. 4 (December 26, 2013): 398–402. http://dx.doi.org/10.7897/2321-6328.01425.

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42

Khan, Haroon Latif, Yousaf Latif Khan, Rameen Makhdoom, and Abdul Rahman Khawaja. "IN VITRO FERTILIZATION." Professional Medical Journal 23, no. 09 (September 10, 2016): 1138–44. http://dx.doi.org/10.29309/tpmj/2016.23.09.1711.

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Various ovarian reserve tests were developed to estimate the ovarian reserve andpredict about the outcome in subfertile females undergoing evaluation for assisted reproduction.FSH and AMH levels are considered to be good ovarian reserve indicators along with antralfollicle count. Objectives: To explore relationship of AMH and FSH in patients undergoing IVFwith respect to ovarian reserve and outcome of the treatment. Study Design: Prospective cohort.Study Period: 1st January 2015 to 31st December 2015. Place of study: Lahore Institute ofFertility and Endocrinology, Hameed Latif Hospital, Lahore Material and Methods: In 346 IVF/ICSI patients after anthropometric measurements and transvaginal ultrasound antral folliclecount were assessed in each ovary. For the hormone measurements blood samples were takenduring the early follicular phase of menstrual cycle. Clinical pregnancy was also visualizedthrough transvaginal ultrasound. Results: From the 346 IVF/ICSI patients 89 (25.79%) clinicalpregnancies resulted. The mean age in pregnant group was 32.89 ± 2.99 years and in nonpregnantgroup was 33.62 ±4.36. Mean FSH and AMH in pregnant group was 6.38 ±2.38,3.27 ±1.86 and in non- pregnant group was 7.54±3.76, 2.72 ± 1.82 respectively. Age andFSH are significantly associated with each other (p-vale = 0.000) and mostly patients had FSHbelow 9(mIU/mL). Age and AMH are significantly associated with each other (p-vale = 0.000)and mostly patients had AMH above 1.5 (ng/mL). Conclusions: Better pregnancy rate wasassociated with FSH below than 9 (mIU/mL) and AMH above 1.5 (ng/mL).

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Skevaki, C., V. Härtel, and H. Renz. "In-vitro-Allergiediagnostik." Allergologie 44, no. 10 (October 1, 2021): 795–808. http://dx.doi.org/10.5414/alx02251.

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44

Gross, Michael. "In vitro conservation." Current Biology 31, no. 18 (September 2021): R1065—R1068. http://dx.doi.org/10.1016/j.cub.2021.09.020.

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Grout, B. W. V. "CONSERVATION IN VITRO." Acta Horticulturae, no. 289 (April 1991): 171–78. http://dx.doi.org/10.17660/actahortic.1991.289.45.

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Yablonskaya, M. I., M. S. Gins, and M. A. Molchanova. "In vitro biotization." RUDN Journal of Agronomy and Animal Industries, no. 1 (2016): 15–20. http://dx.doi.org/10.22363/2312-797x-2016-1-15-20.

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47

Anderson, Diana. "In Vitro Models." Drug Safety 5, Supplement 1 (1990): 27–39. http://dx.doi.org/10.2165/00002018-199000051-00006.

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48

Jansen, Robert P. S., John C. Anderson, Inge Radonic, Robert C. Lyneham, and Warwick R. S. Birrell. "In‐vitro fertilization." Medical Journal of Australia 145, no. 1 (July 1986): 58. http://dx.doi.org/10.5694/j.1326-5377.1986.tb113756.x.

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Trounson, Alan, and Carl Wood. "In‐vitro fertilization." Medical Journal of Australia 146, no. 7 (April 1987): 338–40. http://dx.doi.org/10.5694/j.1326-5377.1987.tb120290.x.

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50

Dawson, Karen. "In‐vitro fertilization." Medical Journal of Australia 147, no. 2 (July 1987): 106. http://dx.doi.org/10.5694/j.1326-5377.1987.tb133291.x.

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