Academic literature on the topic 'In vitro standardization'

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Journal articles on the topic "In vitro standardization"

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Yman, Lars. "Standardization of in vitro methods." Allergy 56, s67 (April 2001): 70–74. http://dx.doi.org/10.1111/j.1398-9995.2001.00921.x.

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Goggs, Robert, Antonio Borrelli, Benjamin M. Brainard, Daniel L. Chan, Armelle de Laforcade, Isabelle Goy-Thollot, Karl E. Jandrey, et al. "Multicenter in vitro thromboelastography and thromboelastometry standardization." Journal of Veterinary Emergency and Critical Care 28, no. 3 (March 31, 2018): 201–12. http://dx.doi.org/10.1111/vec.12710.

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Chander, NGopi. "Standardization of in vitro studies." Journal of Indian Prosthodontic Society 16, no. 3 (2016): 227. http://dx.doi.org/10.4103/0972-4052.186399.

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Pooja, A., Sapna Panwar, A. K. Tiwari, and Gunjeet Kumar. "Standardization of in vitro regeneration protocol in annual chrysanthemum." Indian Journal of Horticulture 78, no. 1 (2021): 46–52. http://dx.doi.org/10.5958/0974-0112.2021.00007.4.

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Ahmad, Sayeed, Mhaveer Singh, MohammadA Khan, MasoodS Khan, and SH Ansari. "Standardization and in vitro antioxidant activity of jatamansi rhizome." Journal of Pharmacy and Bioallied Sciences 7, no. 4 (2015): 275. http://dx.doi.org/10.4103/0975-7406.168025.

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Malvicini, R., D. Santa Cruz, C. Sanmartin, N. Pacienza, and G. Yannarelli. "Bioassay standardization to assess exosomes antiinflammatory activity in vitro." Cytotherapy 22, no. 5 (May 2020): S24. http://dx.doi.org/10.1016/j.jcyt.2020.03.500.

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Morosato, Federico, Francesco Traina, and Luca Cristofolini. "Standardization of hemipelvis alignment for in vitro biomechanical testing." Journal of Orthopaedic Research® 36, no. 6 (December 19, 2017): 1645–52. http://dx.doi.org/10.1002/jor.23825.

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Kaur, Harpreet, Jaismeen Kaur, and Bhagwant Singh Chahil. "in vitro protocol standardization for growth and rooting in strawberry." Journal of Krishi Vigyan 9, no. 1 (2020): 193–201. http://dx.doi.org/10.5958/2349-4433.2020.00158.0.

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Schendel, Dolores J., Rudolf Wank, and Bo Dupont. "Standardization of the Human in vitro Cell-mediated Lympholysis Technique." Tissue Antigens 13, no. 2 (December 11, 2008): 112–20. http://dx.doi.org/10.1111/j.1399-0039.1979.tb01146.x.

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Sudha, V., K. L. Niraimathi, R. Lavanya, C. David Raj, and P. Brindha. "Chemical Standardization and in vitro Cytotoxic Studies on Nellikai lehyam." Asian Journal of Chemistry 26, no. 12 (2014): 3679–82. http://dx.doi.org/10.14233/ajchem.2014.17053.

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Dissertations / Theses on the topic "In vitro standardization"

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NUNES, Cristina Freitas. "Atividade virucida de um extrato etanólico de própolis verde in vitro e in vivo." Universidade Federal de Pelotas, 2011. http://repositorio.ufpel.edu.br/handle/ri/2567.

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Currently, the drug industry looks for new drugs based on natural products, for the production of drugs more efficient, for which the microorganisms did not show resistance to both humans and animals. A natural product that has been the subject of intense pharmacological and chemical studies by scientists for the control of diseases is propolis, a resinous substance produced by honeybees from exudates collected from different parts of the plant, which has been used for centuries in popular medicine due to its therapeutic properties. Chemical studies revealed the complex chemical composition, identifying in some cases more than 300 components including various bioactive phenolic compounds responsible for the virucidal action. This work initially describes the standardization of an ethanol extract of green propolis (EEPV), where the chemicals were identified by high performance liquid chromatography (HPLC), phytochemical characterization by thin layer chromatography (TLC), soluble solids, content of phenolics and flavonoids and antioxidant activity by 2.2 diphenil picryl hydrazyl (DPPH). The EEPV was also evaluated in vitro and in vivo for their capacity lentogenic virucidal against a strain of the virus of Newcastle disease (NDV) at two different temperatures (22 and 37 ° C), 5 incubation periods (0, 1, 2, 4 and 8 hours) of NDV in five different concentrations of EEPV (4000μg/dose, 400μg/dose, 40μg/dose, and 4μg/dose 0μg/dose). The EEPV standard is within the standards required by the MAP, with high levels of phenolics and flavonoids (12.93 and 6.05% respectively) as shown by HPLC, which identified high concentrations of phenolic acids (p-coumaric acid, hydroxycinnamic acid diprenyl , cinâmino acid derivatives), which are assigned the antibacterial, antioxidant, antiviral and virucidal. This extract showed dose-dependent virucidal activity (4000μg/dose e 400μg/dose) and time of incubation with the virus (2 hour). The inhibitory activity of EEPV against the strain of NDV lentogenic found in the present study suggests the use of this extract as an alternative to fight the infection by this virus.
Atualmente, a indústria farmacêutica busca novos medicamentos com base em produtos naturais, visando à produção de fármacos mais eficientes, para os quais os microrganismos não apresentem resistência, tanto para humanos quanto para animais. Um dos produtos naturais que tem sido objeto de intensos estudos farmacológicos e químicos por cientistas para o controle de enfermidades é a própolis, uma substância resinosa produzida por abelhas melíferas a partir de exsudatos coletados em diferentes partes das plantas, que tem sido utilizada durante séculos na medicina popular devido as suas propriedades terapêuticas. Estudos químicos revelaram a complexa composição da própolis, identificando em alguns casos mais de 300 componentes, incluindo vários compostos bioativos fenólicos responsáveis pela ação virucida. Este trabalho inicialmente descreve a padronização de um extrato etanólico de própolis verde (EEPV), onde foram identificados os compostos químicos por cromatografia liquida de alta eficiência (CLAE), caracterização fitoquímica por cromatografia em camada delgada (CCD), teor de sólidos solúveis, teor de fenóis e flavonóides totais e atividade antioxidante por 2,2 diphenil picril hidrazil(DPPH). O EEPV foi avaliado também in vitro e in vivo, quanto a sua capacidade virucida contra uma cepa lentogênica do vírus da doença de Newcastle (NDV) em duas temperaturas distintas (22 e 37°C), 5 períodos de incubação (0, 1, 2, 4 e 8 horas) do NDV em 5 concentrações de EEPV distintos (4000μg/dose, 400μg/dose, 40μg/dose, 4μg/dose e 0μg/dose). O EEPV padronizado está dentro dos padrões requisitados pelo MAPA, com altos níveis de fenóis e flavonóides totais (12.93 e 6,05% respectivamente), comprovado por CLAE, o qual identificou altas concentrações de ácidos fenólicos (ácido p-cumárico, ácido diprenil hidroxicinâmico, derivados do ácido cinâmino), os quais são atribuídos as propriedades antibacteriana, antioxidante, antiviral e virucida. Este extrato apresentou atividade virucida dependente da dose (4000μg/dose, 400μg/dose) e do tempo de incubação com o vírus (2 horas). A atividade inibitória do EEPV contra a cepa lentogênica de NDV, encontrada no presente estudo sugere a utilização deste extrato como uma alternativa no combate a infecções por este vírus.
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LUCO, DAYANE P. "Padronização de técnicas de isolamento de células de Langerhans imaturas e desenvolvimento de um modelo tridimensional de pele humana para testes de sensibilidade in vitro." reponame:Repositório Institucional do IPEN, 2014. http://repositorio.ipen.br:8080/xmlui/handle/123456789/23179.

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Dissertação (Mestrado em Tecnologia Nuclear)
IPEN/D
Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP
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STANCO, DEBORAH. "Toward personalized medicine: human adipose-derived stem cells (ASCs) and xenogenic-free media as enabling tools in tendon tissue engineering strategies." Doctoral thesis, Politecnico di Torino, 2020. http://hdl.handle.net/11583/2839845.

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Books on the topic "In vitro standardization"

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S, Ambesi-Impiombato F., and Perrild H, eds. FRTL-5 today: Proceedings of the First International Workshop on Characterization and Standardization of an In Vitro Thyroid Cell System, Udine, Italy, 26-28 October 1988. Amsterdam: Excerpta Medica, 1989.

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Book chapters on the topic "In vitro standardization"

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Stacey, Glyn N., and Thomas Hartung. "Availability, Standardization and Safety of Human Cells and Tissues for Drug Screening and Testing." In Drug Testing in vitro, 229–50. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2006. http://dx.doi.org/10.1002/9783527609611.ch9.

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Raja, Pavan M. V., Ghislaine Lacroix, Jacques-Aurélien Sergent, Frédéric Bois, Andrew R. Barron, Enrico Monbelli, and Dan Elgrabli. "Nanotoxicology: Role of Physical and Chemical Characterization and RelatedIn Vitro,In Vivo, andIn SilicoMethods." In Metrology and Standardization of Nanotechnology, 363–80. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2017. http://dx.doi.org/10.1002/9783527800308.ch23.

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Lima-Filho, José Vitor, and Rossana de Aguiar Cordeiro. "In Vitro and In Vivo Antibacterial and Antifungal Screening of Natural Plant Products: Prospective Standardization of Basic Methods." In Springer Protocols Handbooks, 275–91. New York, NY: Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4614-8636-7_17.

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Schmitt, K., S. Rosenkranz, F. Platzer, G. Jagarzewskj, M. Kästner, A. Schroda, R. Seitz, and J. Dodt. "Standardization of the Thrombin-Fibrinogen Clotting Time (TFCT) — An In Vitro Test on Thrombogenicity of Prothrombin Complex Concentrates (PCCs)." In 28. Hämophilie-Symposion Hamburg 1997, 125–34. Berlin, Heidelberg: Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-642-59915-6_17.

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Ekwall, Bjorn, and Frank A. Barile. "Standardization and Validation." In Introduction to In Vitro Cytotoxicology, 189–208. CRC Press, 2019. http://dx.doi.org/10.1201/9780429275487-11.

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Bhatt, Deepa, M. K. Tripathi, M. Vidhya Sankar, Sushma Tiwari, Mohini Sharma, Niraj Tripathi, and Sharad Tiwari. "Standardization of In-vitro Regeneration Protocol in Gerbera jamesonii Bolus Ex Hooker F." In Current Topics in Agricultural Sciences Vol. 7, 105–21. Book Publisher International (a part of SCIENCEDOMAIN International), 2022. http://dx.doi.org/10.9734/bpi/ctas/v7/2303b.

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Borziak, Kirill, Irena Parvanova, and Joseph Finkelstein. "Introducing a Platform for Integrating and Sharing Stem Cell Research Data." In Studies in Health Technology and Informatics. IOS Press, 2021. http://dx.doi.org/10.3233/shti210186.

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Advancements in regenerative medicine have highlighted the need for increased standardization and sharing of stem cell products to help drive these innovative interventions toward public availability and to increase collaboration in the scientific community. Although numerous attempts and numerous databases have been made to store this data, there is still a lack of a platform that incorporates heterogeneous stem cell information into a harmonized project-based framework. The aim of the platform described in this study, ReMeDy, is to provide an intelligent informatics solution which integrates diverse stem cell product characteristics with study subject and omics information. In the resulting platform, heterogeneous data is validated using predefined ontologies and stored in a relational database. In this initial feasibility study, testing of the ReMeDy functionality was performed using published, publically-available induced pluripotent stem cell projects conducted in in vitro, preclinical and intervention evaluations. It demonstrated the robustness of ReMeDy for storing diverse iPSC data, by seamlessly harmonizing diverse common data elements, and the potential utility of this platform for driving knowledge generation from the aggregation of this shared data. Next steps include increasing the number of curated projects by developing a crowdsourcing framework for data upload and an automated pipeline for metadata abstraction. The database is publically accessible at https://remedy.mssm.edu/.
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"Good cell culture practice: good laboratory practice in the cell culture laboratory for the standardization and quality assurance of in vitro studies." In Cell Culture Models of Biological Barriers, 138–46. CRC Press, 2002. http://dx.doi.org/10.1201/9780203219935-14.

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Oliver, Anthony R. "Laboratory Quality Control and Accreditation." In Tutorial Topics in Infection for the Combined Infection Training Programme. Oxford University Press, 2019. http://dx.doi.org/10.1093/oso/9780198801740.003.0019.

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According to the International Organization for Standardization (ISO), the ‘Medical laboratories—Requirements for quality and competence (ISO 15189:2012) BS EN ISO 15189:2012’ accreditation is defined as ‘a procedure by which an authoritative body gives formal recognition that an organization is competent to carry out specific tasks’. Accreditation is delivered by the ‘competent authority’ based on a set of defined standards and the continual internal audit of the laboratory processes and infrastructure against these standards to achieve conformance. Additionally, the ‘competent authority’ periodically undertakes assessments to ensure compliance with the standards. These assessments vary in frequency and nature depending upon the assessment body. In some instances (e.g. UK Accreditation Service, UKAS), the assessments are annual and based on a four- year cycle covering the whole laboratory repertoire and infrastructure. The HSE is responsible for the inspection and licencing of microbiological containment level 3 and 4 facilities. The HTA is responsible for legal registration of laboratories that process and store human tissue, and is mainly histology related. The MHRA provides guidelines on good laboratory practice, good clinical practice, good clinical laboratory practice, and good manufacturing practice, largely around clinical trial work. It is also responsible for accreditation of blood transfusion laboratories. Finally, it provides guidance on the In Vitro Diagnostic Medical Device Directive (IVDMDD, 98/ 79/ EC) and the regulation of medical ‘devices’ including diagnostic devices, where a ‘device’ is defined as including reagent kits and analytical platforms. EFI provides guidance and standards for transplantation and tissue typing laboratories across Europe. Until 2009, CPA provided accreditation for the majority of UK pathology services. CPA was acquired by the UK Accreditation Service in 2009. UKAS is a government- appointed national accreditation body for the UK that is responsible for certification, testing, inspection, and calibration services, and is the competent authority for all ISO standards, not just pathology. It covers various sectors, including healthcare, food production, energy supply, climate change, and personal safety. The majority of UK pathology services will be UKAS ISO15189 accredited by 2018, including transitional ‘dual’ CPA standards/ ISO15189 accreditation between 2015 and 2018. It also provides ISO22870:2006 accreditation that is point of care specific, as well as ISO17025:2005, which applies to calibration standards.
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Conference papers on the topic "In vitro standardization"

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Singhera, G. K., T. Guo, J. M. Leung, and D. R. Dorscheid. "Standardization of Airway Epithelial 3D Culture for In Vitro Studies." In American Thoracic Society 2020 International Conference, May 15-20, 2020 - Philadelphia, PA. American Thoracic Society, 2020. http://dx.doi.org/10.1164/ajrccm-conference.2020.201.1_meetingabstracts.a1300.

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Silva, Andréa, Tamiris Azamor, Leonardo Batista-Silva, Nathalia Benigno, Thyago Calvo, Camilla Bayma, Denise Matos, and Milton Moraes. "Standardization of an in vitro assay for the evaluation of a candidate yellow fever vaccine." In IV International Symposium on Immunobiologicals & VII Seminário Anual Científico e Tecnológico. Instituto de Tecnologia em Imunobiológicos, 2019. http://dx.doi.org/10.35259/isi.sact.2019_32551.

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Hoppensteadt, D., J. Fareed, J. M. Walenga, R. M. Emnuele, and A. Racanelli. "DIFFERENT LOW MOLECULAR WEIGHT HEPARINS (LMWHs) ARE NOT IDENTICAL IN THE IN VITRO SCREENING REGARDLESS OF POTENCY ADJUSTMENT." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643227.

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There is considerable controversy in the standardization and potency evaluation of low molecular weight heparins (LMWHs).Since these agents are produced by fractionation or depolymerization procedures, the resulting material shows marked compositional variations regardless of the similarity in molecular weight. In order to address the question “are different LMWHs the same?”, we utilized a uniform multiparametric in vitro test battery. Seven commercial LMWHs Choay Fraxiparine (CY 216), Choay CY 222, Novo LHN, Kabi Fragmin, Opocrin 2123 (OP), Hepar RD 11885 (RD), Pharmuka Enoxaparine (PK) and an unfractionated porcine mucosal heparin (PMH) were studied at equigravimetric amounts in established assays. PMH and LMWHs were ranked acdording to the dose-dependent effects and the results are summarized in the following:As evident from the above data, each of the agents studied showed a distinct profile in the in vitro test system studied. This data suggests that LMWHs are individual drugs with marked differences in in vitro actions, which may not be the true determinants of the relative safety/efficacy in clinical settings.
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Zwagerman, Ralph. "Development of ISO18363-4 / AOCS Cd29f-2021: A new standardized method to quantify MCPDE and GE in edible oils." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/dqcb6439.

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In August 2021 the International Organization for Standardization (ISO) Â has published part 4 to the ISO 18363 series of methods to quantify MCPD- and glycidyl esters in edible oils. AOCS will follow by publishing the method as AOCS Cd 29f-2021 in 2022. This new method describes a high throughput method for the quantification of these process contaminants, for which the EU has recently updated its legislation on the maximum levels allowed in edible oils. In short, its chemistry is based on fast alkaline transesterification of the fat matrix, which also cleaves the MCPDE and GE esters before the analytes are derivatization and analysis by GC-MS/MS. An in vitro correction for glycidol overestimation, which can occur in alkaline environment, is included. In the fall of 2018 both ISO and AOCS technical committees agreed to standardize the method in cooperation. An overview is given of the standardization project together with statistical data collected during the collaborative study together with a short introduction to the chemistry of the method.
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Suor-Cherer, S., H. Bui Thi Quynh, and P. Caillis. "Standardization of an in vitro spectrophotometric method for the evaluation of the ammonia-binding properties of plant extracts rich in saponins." In 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP. © Georg Thieme Verlag KG, 2019. http://dx.doi.org/10.1055/s-0039-3399745.

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Wehmeier, A., and W. Schneider. "FACTORS AFFECTING PLATELET VOLUME ANALYSIS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643538.

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Parameters of platelet volume have become widely available with the introduction of automated platelet counters. However, variant sample processing and in vitro platelet activation have prevented standardization of platelet volume analysis. We investigated the influence of anticoagulation, storage, temperature, and the presence or absence of RBC on platelet volume. Mean platelet volume (MPV) and the mode of the distribution were calculated from the platelet volume distribution curve recorded with the impedance method and plotted in 27 classes between 1.2 and 22 fl. The effects of EDTA (.335%), citrate (.38%), citrate (.38%)/glutaraldehyde (.125%) and a cocktail containing citrate, forskolin, indomethacin and theophyllin were determined 10, 30, 60, 120 and 240 min after blood collection. Tests were made at 4, 20° and 37°C in whole blood and platelet-rich plasma (PRP)from 6 healthy subjects. Platelet volume was strongly dependent on the anticoagulant in a time-dependent manner. MPV was lowest with citrate/glutaraldehyde and highest with EDTA. The maximum difference was 30% at 60 min both in whole blood and PRP. However, this was true only at 4 and 20°C. At 37°C, there was a constant rise in MPV using citrate/glutaraldehyde exceeding volume changes seen with the other anticoagulants. Platelet volume was higher in whole blood as compared to PRP. The difference was dependent on the anticoagulant used and the incubation time (1.8 fl for EDTA and 1.35 fl for citrate/glutaraldehyde, at 60 min). To determine the influence of platelet loss due to PRP preparation on this effect, we determined platelet volume in parallel from whole blood, PRP and the platelet population separated from whole blood by a linear Percoll gradient (n=5, recovery 94%). MPV was 7.6 fl in whole blood, 5.4 fl in PRP and 6.8 fl from the gradient (anticoagulant: citrate/glutaraldehyde). Platelet volume parameters highly depend on anticoagulation, incubation time and temperature. For clinical studies we recommend anticoagulation with citrate/glutaraldehyde and measurement within 2 hr at room temperature in whole blood.
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