Dissertations / Theses on the topic 'In Vitro Liver Models'
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Vu, Lucas Trung. "Proteomic Analysis of Three Dimensional Organotypic Liver Models." Diss., Virginia Tech, 2015. http://hdl.handle.net/10919/77033.
Full textPh. D.
Granitzny, Anne [Verfasser]. "In vitro/ex vivo liver models for the prediction of idiosyncratic drug-induced liver injury / Anne Granitzny." Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2017. http://d-nb.info/1150192496/34.
Full textTirnitz-Parker, Janina Elke Eleonore. "Primary culture and immortal cell lines as in vitro models to evaluate the role of TWEAK signalling in hepatic oval cells /." Connect to this title, 2007. http://theses.library.uwa.edu.au/adt-WU2008.0039.
Full textHeslop, James. "Investigating novel methods of enhancing in vitro models of drug induced liver injury." Thesis, University of Liverpool, 2015. http://livrepository.liverpool.ac.uk/2053229/.
Full textChang, Robert Chao Sun Wei. "Biofabrication of three-dimensional liver cell-embedded tissue constructs for in vitro drug metabolism models /." Philadelphia, Pa. : Drexel University, 2009. http://hdl.handle.net/1860/3069.
Full textRegan, Sophie Louise. "In Vitro and In Vivo models for the investigation of drug bioactivation and drug-induced liver injury." Thesis, University of Liverpool, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.510967.
Full textBrabyn, Caroline Jane. "Development and characterization of an in vitro model for liver homeostasis." Thesis, University of Ottawa (Canada), 1994. http://hdl.handle.net/10393/6906.
Full textDouglas, O. "An evaluation of in vitro models for the assessment of mitochondrial toxicity within Drug Induced Liver Injury (DILI)." Thesis, University of Liverpool, 2017. http://livrepository.liverpool.ac.uk/3012327/.
Full textSams, Craig. "In vitro human liver metabolism of some industrial solvents and pesticides and incorporation of metabolic parameters into mathematical models." Thesis, University of Sheffield, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.434530.
Full textEssaouiba, Amal. "Development of a liver-pancreas in vitro model using microfluidic organ-on-chip technologies." Thesis, Compiègne, 2020. http://www.theses.fr/2020COMP2573.
Full textDiabetes mellitus (DM) or the so called disease of the century is a life threatening dysfunction that affects the endocrine system. The mechanisms underlying the break in the feedback loop that regulates the metabolism and the consequent diabetes induction are not fully known. Understanding the mechanisms of insulin action is therefore crucial for the further development of effective therapeutic strategies to combat DM. Accordingly, it is imperative to find a robust and reliable model for diabetes research able to overcome the limitations of traditional 2D in vitro cell culture and animal experimentation. The aim of this thesis is to develop a new liver‐pancreas co‐culture model using advanced microphysiological systems (MPs) to tackle more effectively the mechanism involving the hepatic and pancreatic endocrine regulation. This work highlights the power of multi organ‐on‐chip systems that combines the advanced 3D‐cell compartmentalization, microfluidics and induced pluripotent stem cells (iPSC) technology to achieve a high biological complexity and functions that are rarely reproduced by only one of these tissue engineering technologies
Boulais, Lilandra. "Cryogel-integrated hepatic cell culture microchips for liver tissue engineering." Thesis, Compiègne, 2020. http://www.theses.fr/2020COMP2561.
Full textToday, one of the challenges for the pharmaceutical industry is to develop accurate in vitro liver models to improve the predictability of preclinical studies, in particular the study of the toxicity and efficacy of drug candidates. In recent years, tissue engineering, a multidisciplinary approach to develop tissues, has led to the development of new cell culture methods. Among them, cell cultures in 3D or in perfusion allowed to obtain hepatic activities similar to those observed in vivo. The objective of this thesis is to combine these two cell culture methods to create an even more accurate in vitro liver model. To do so, we are seeking to develop an alginate cryogel integrated into a microchip with mechanical properties adaptable to those of the liver depending on the physiological state to be reproduced (healthy or pathological liver).In the first part, we develop and characterize the alginate cryogel at the microscopic and macroscopic level, outside (cylindrical samples) and then inside the biochip. Three parameters are studied here: the cryopolymerization temperature, the alginate concentration and the quantity of cross-linking agents. Mechanical properties, porosity, absorption, pore interconnectivity and flow resistance are analyzed. The second part aims to culture liver cells within this new device. For this feasibility study the HepG2/C3A cell line is used. The results show viable and functional cells (albumin production, APAP transformation). In addition, we observe a 3D tissue structure, which is maintained after removal of the alginate cryogel. The last part aims to complexify the hepatic model, in particular by co-cultures. To get closer to the sinusoid structure, liver cells are cultured with endothelial cells (HUVEC) according to two approaches. In addition, the possibility to follow circulating tumor cells (MDA-MB-231) in the system is studied
Chen, Liqiong. "The development of a novel in vitro model of human liver for the study of disease pathogenesis." Thesis, University of Nottingham, 2010. http://eprints.nottingham.ac.uk/11020/.
Full textJohann, Esther [Verfasser], Dieter [Akademischer Betreuer] Schrenk, and Stefan Otto [Akademischer Betreuer] Müller. "Improved early in vitro prediction of drug-induced liver injury in man: assessment of novel 3D hepatic models / Esther Johann ; Dieter Schrenk, Stefan Otto Müller." Kaiserslautern : Technische Universität Kaiserslautern, 2018. http://d-nb.info/1170154018/34.
Full textDanoy, Mathieu. "Development of a physiologically-relevant in-vitro microfluidic model for monitoring of pancreatic cancer cells interactions with the liver." Thesis, Lille 1, 2017. http://www.theses.fr/2017LIL10093/document.
Full textThe cancer metastatic process and its understanding have been a major topic of interest for researchers in the past. Using in-vitro models in both standard culture conditions and in microfluidic devices, we investigated the feasibility of such models in the representation of the physiological in-vivo situation. We developed a hierarchical coculture model in PDMS plates, composed of hepatocytes, pericytes and endothelial cells. In different culture conditions, the influence of the different cells composing the model on the adhesion of cancer cells and promyeloblastic cells was investigated as well as the influence on the inflammatory state of the culture. To reproduce the in-vivo blood flow and shear stress to which the endothelial cells and the adhering cells are subjected, the model was then transferred into a microfluidic biochip. The device was composed of three channels, separated by micropillars and which could be filled independently one from another. Pericytes embedded in a hydrogel, hepatocytes, endothelial cells and finally pancreatic cancer cells could be inserted successively to reproduce the in-vivo hierarchical situation. Cells were found to viable after the culture and markers related to the liver and inflammation to be expressed. The influence of the presence of hepatocytes and pericytes was investigated by varying the culture conditions. It was found that pancreatic cancer cells were attracted by the cells in other channels in coculture. The established models lay the bases for more complex and relevant systems that could complement their in-vivo counterparts in the drug discovery process
Lohr, Christiane [Verfasser], and Dieter [Akademischer Betreuer] Schrenk. "Effects of Polychlorinated Dibenzo-p-Dioxins, Polychlorinated Dibenzofurans, and Polychlorinated Biphenyls in Human Liver Cell Models (in vitro) and in Mice (in vivo) / Christiane Lohr. Betreuer: Dieter Schrenk." Kaiserslautern : Technische Universität Kaiserslautern, 2013. http://d-nb.info/1045604054/34.
Full textEuler, Henrik von. "Electrochemical treatment of tumours /." Uppsala : Dept. of Small Animal Clinical Sciences, Swedish Univ. of Agricultural Sciences ([Institutionen för kirurgi och medicin - smådjur], Sveriges lantbruksuniv.), 2002. http://epsilon.slu.se/v133.pdf.
Full textLlamas, Vidales Jose Ricardo. "Use of a 3D liver microreactor as an in vitro model for the study of bile acid synthesis and hepatobiliary circulation." Thesis, Massachusetts Institute of Technology, 2009. http://hdl.handle.net/1721.1/61226.
Full textCataloged from PDF version of thesis.
Includes bibliographical references (p. 149-165).
The liver regulates a myriad of vital functions including bile acid synthesis, hepatobiliary circulation, cholesterol homeostasis, drug metabolism, etc. This thesis focuses on the use of a 3D in vitro model of liver to study the effects of compounds and culture conditions on hepatobiliary transport and bile acid synthesis. In order to achieve this goal, protocols were developed to perform hepatic transport studies of bile acids in perfused 3D primary rat hepatocyte cultures. An established 2D sandwich culture model was used as a foundation for evaluation of variations in protocol parameters including culture medium composition and assay incubation times. In 2D sandwich cultures, dexamethasone (DEX) was essential for the formation of canalicular networks, whereas epidermal growth factor (EGF) disrupted the formation of these networks. Strikingly, EGF promotes cellular re-polarization and canalicular network formation in perfused 3D cultures, in contrast to its effect on 2D cultures. Perfused 3D cultures were found to have greater bile acid transport capabilities and closer to in vivo expression of certain liver transporters than 2D sandwich cultures on day 7. Perfused 3D cultures also had greater bile acid synthesis on day 7 than 2D cultures, however this was inhibited by EGF in both cultures. A numerical model was also developed for the reduction of experimental measurements necessary to determine pharmacokinetic parameters of hepatic transport, thus allowing for quantitative comparison of the effects of different culture conditions and culture platforms on transporter activity. The result of this thesis is the adaptation of a system for the study of hepatic transport and bile acid synthesis to 3D cultures. This system has also been previously shown to maintain other liver functions such as drug metabolism, and the work of this thesis thus allows for the concerted study of all these functions and potentially others.
by Jose Ricardo Llamas Vidales.
Ph.D.
Ekins, Sean. "Maintenance and cryopreservation of xenobiotic metabolism in precision-cut liver slices : evaluation of an alternative in vitro model to isolated hepatocytes." Thesis, University of Aberdeen, 1996. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU543199.
Full textShibany, Khaled Ayad S. "The development of an in vitro model using equine hepatocytes and liver microsomes for the study of drug metabolism in the horse." Thesis, University of Nottingham, 2018. http://eprints.nottingham.ac.uk/51310/.
Full textCornu, Raphaël. "Nanoparticules et santé : de grandes promesses thérapeutiques, mais pour quel risque ?" Thesis, Bourgogne Franche-Comté, 2019. http://www.theses.fr/2019UBFCE018.
Full textNanoparticles are defined as spherical structures with a maximum diameter of 100 nanometers. The application fields of nanoparticles including food and pharmaceutical industries are extremely broad. Human can be exposed daily to nanoparticles through various administration routes (oral, intravenous, pulmonary and cutaneous). Due to their size at the nanoscale, nanoparticles have unique physicochemical, inducing strong interactions with the biological environment. These features were widely exploited for the conception of nanomedicines for the diagnosis and the therapy. However, issues relative to their biological toxicity were addressed in the same time. This thesis work aims to investigate the potential toxicity of nanoparticles. Toxicological evaluation was performed using cell models adapted for the systemic and the oral routes. Mechanisms involved in the nanotoxicity were studied to identify toxicity factors. The first part of the work focused on the in vitro and in vivo hepatic toxicity of PLGA and silica nanoparticles. PLGA nanoparticles are used as drug carriers while silica nanoparticles play the role of anticaking agent in food industry and of pharmaceutical excipients. Their effects on the liver function and especially on the cytochrome P450 activity were investigated. The second part of the work consisted to study the impact of silica nanoparticles on the intestinal barrier, especially on the paracellular permeability and the integrity of the barrier. By emphasizing interspecies differences or the protective role of mucus, this project demonstrated that the choice of toxicological tools was crucial for a predictive nanotoxicity evaluation. Size, surface properties and composition were identified as major toxicity factors
Georgiou, Leonidas. "DCE-MRI assessment of hepatic uptake and efflux of the contrast agent, gadoxetate, to monitor transporter-mediated processes and drug-drug interactions : in vitro and in vivo studies." Thesis, University of Manchester, 2015. https://www.research.manchester.ac.uk/portal/en/theses/dcemri-assessment-of-hepatic-uptake-and-efflux-of-the-contrast-agent-gadoxetate-to-monitor-transportermediated-processes-and-drugdrug-interactions-in-vitro-and-in-vivo-studies(d4b3bc62-8636-470b-90ae-38e25e7ee7be).html.
Full textTian, Yinghua. "Liver and partial liver transplantation : new models and mechanisms of partial liver graft regeneration /." Zürich, 2005. http://opac.nebis.ch/cgi-bin/showAbstract.pl?sys=000253387.
Full textOrbach, Sophia Michelle. "Multi-Cellular Organotypic Liver Models for the Investigation of Chemical Toxicity and Liver Fibrosis." Diss., Virginia Tech, 2018. http://hdl.handle.net/10919/93313.
Full textPh. D.
Jones, Elizabeth A. "Investigation and in vitro modelling of early liver development." Thesis, University of Newcastle Upon Tyne, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.419699.
Full textMbamalu, Godwin E. "In Vitro Modulation of Rat Liver Glyoxalase II Activity." Thesis, University of North Texas, 1988. https://digital.library.unt.edu/ark:/67531/metadc500992/.
Full textLewis, Andrew L. "Flow cytometric analysis of hepatocyte proliferation in vitro." Thesis, University of Nottingham, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.288990.
Full textLi, Wan-Chun. "In vitro transdifferentiation of liver into functional pancreatic-like cells." Thesis, University of Bath, 2006. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.425508.
Full textTuckwell, Daniel S. "In vitro models for degenerative diseases." Thesis, University of Nottingham, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.277478.
Full textThomson, Hazel. "Scale-up and 'in vitro' testing of the Strathclyde bioartificial liver." Thesis, University of Strathclyde, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.426362.
Full textDavies, Catherine Sarah. "In vitro assay of hydroxynaphthoquinones against the liver stages of 'Plasmodium'." Thesis, Imperial College London, 1989. http://hdl.handle.net/10044/1/47401.
Full textAshfaq-Khan, Muhammad [Verfasser]. "Dietary wheat amylase trypsin inhibitors worsen chronic liver disease in preclinical models of non-alcoholic fatty liver disease and liver fibrosis / Muhammad Ashfaq-Khan." Mainz : Universitätsbibliothek Mainz, 2018. http://d-nb.info/1173844449/34.
Full textHayward, Nicola Margaret. "Apoliprotein B metabolism in hamster livers, studied in vitro." Master's thesis, University of Cape Town, 1990. http://hdl.handle.net/11427/27176.
Full textBruschi, Sam A. "Investigations into mechanisms of paracetamol-induced toxicity using ìn vitro' systems /." Title page, abstract and table of contents only, 1987. http://web4.library.adelaide.edu.au/theses/09PH/09phb192.pdf.
Full textPathmanathan, Saidharshini. "Development of in vitro models of cerebral ischaemia." Thesis, University of Oxford, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.249162.
Full textSivathanu, Vivek. "In vitro models for airway epithelial cell culture." Thesis, Massachusetts Institute of Technology, 2013. http://hdl.handle.net/1721.1/81726.
Full textCataloged from PDF version of thesis.
Includes bibliographical references (p. 40-41).
This work is about the development of a physiologically relevant model of the human airway. Various factors such as the cell model, physiochemical factors such as the cell substrate properties including its stiffness, shear stress, stretch, the air-liquid interface and the biochemical factors in the medium influence the biology of the cells. The aim of this work is to closely approximate conditions in an in vivo situation by engineering the above conditions in to the in vitro platform. An assay to introduce the cell substrate properties was developed in a glass bottomed petri dish type culture as well as a microfluidic device culture. The influence of the cell substrate on airway epithelial cell monolayer formation was investigated in detail by changing the stiffness of the substrate independently by changing the gel concentration, the gel formation pH and the height of the gel from a hard substrate. Further, we found that biochemical growth factors have a huge role in cell monolayer formation. A real-time measurement of monolayer integrity using electrical resistance measurements was developed. A shear stress application platform was developed and a stretch application platform was designed. The applications of such a platform with the inclusion of various physiologically relevant factors include the study of physiologic evolution of microbes such as the influenza virus.
by Vivek Sivathanu.
S.M.
Rhodes, Hannah Lucinda. "Genetic analysis and in vitro models of cystinuria." Thesis, University of Bristol, 2015. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.738194.
Full textHurrell, Tracey. "Proteomic assessment of potential in vitro hepatotoxicity models." Thesis, University of Pretoria, 2016. http://hdl.handle.net/2263/59107.
Full textThesis (PhD)--University of Pretoria, 2016.
Pharmacology
PhD
Unrestricted
Kalber, Tammy Louise. "In vivo susceptibility-contrast MRI studies of mouse models of liver metastasis." Thesis, St George's, University of London, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.440446.
Full textKhademhosseini, Alireza. "In vitro study of bone marrow derived progenitor cells in liver-like microenvironments." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp05/MQ62916.pdf.
Full textHammond, John Stotesbury. "Scaffolds for liver tissue engineering : in vitro co-culture & in vivo release." Thesis, University of Nottingham, 2009. http://eprints.nottingham.ac.uk/12556/.
Full textKalezi, Artemis. "Tissue-engineered liver microreactor as an in vitro surrogate assay for gene delivery." Thesis, Massachusetts Institute of Technology, 2007. http://hdl.handle.net/1721.1/38981.
Full textIncludes bibliographical references.
The lack of correlation between in vitro and in vivo gene delivery experiments presents a significant obstacle in the progress of gene therapy studies by preventing the extrapolation of successful cell culture results into animals. This phenomenon has also been documented in the specific case of liver where standard hepatocyte culture systems fail to reliably predict the in vivo performance of gene delivery vectors. This is possibly a consequence of the loss of differentiated phenotype that these cells undergo when they are dissociated from their in vivo environment and cultured in vitro. This problem underscores the necessity for better in vitro models that can mimic the physiological environment and responses of in vivo liver tissue. This thesis aimed at developing an alternative in vitro gene delivery assay based on the Tissue-Engineered Liver Microreactor, a culture system designed to facilitate the morphogenesis of three-dimensional tissue-like structures from isolated liver cells under continuous perfusion, maintain cell viability and hepatic functionality for long-term culture periods and enable repeated in situ observation with microscopy. We developed experimental assays to non-invasively detect and quantify gene delivery efficiency in the 3D environment of the microreactor culture based on the application of 2-photon microscopy and spectroscopy.
(cont.) These techniques provide a convenient platform for comparative analysis of different vectors. Our main objective was to compare the gene delivery efficiency of an adenoviral vector (Ad5-CMV-EGFP) in the microreactor system and 2D hepatocyte monolayer culture. Quantitative assays were developed based on Real-Time PCR and RT-PCR to measure the levels of Ad vector uptake and transgene expression. The Ad mass transport in both systems was mathematically modeled to estimate the Ad uptake constant as a basis for comparison of delivery efficiency. This parameter was found to be significantly higher in the microreactor system, suggesting a more efficient mechanism of Ad internalization. Moreover, gene expression was measured in terms of transgene mRNA levels; the ratio of gene expression relative to Ad uptake was estimated as the basis for comparison of vector transcription efficiency. No significant difference was found between the 2 systems. These results provide some evidence that a more physiological culture system can yield different information (potentially more relevant to the in vivo situation) compared to standard in vitro culture.
by Artemis Kalezi.
Ph.D.
Gunawardhana, Lhanoo 1959. "Human liver slices: An in vitro system for determination of N-acetylation and acetylator status." Thesis, The University of Arizona, 1989. http://hdl.handle.net/10150/291394.
Full textLarkin, Adam Lyston. "The Design of Three-Dimensional Multicellular Liver Models Using Detachable, Nanoscale Polyelectrolyte Multilayers." Diss., Virginia Tech, 2012. http://hdl.handle.net/10919/77190.
Full textPh. D.
Mahmood, Tahir. "Models of tissue bonding to bioactive glass in vitro." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape3/PQDD_0027/MQ50351.pdf.
Full textChang, Cynthia J. "In vivo and in vitro models of distraction osteogenesis." Thesis, University of Oxford, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.558214.
Full textLomas, Emma C. "Investigation of post-mortem redistribution using in vitro models." Thesis, University of Huddersfield, 2018. http://eprints.hud.ac.uk/id/eprint/34635/.
Full textWilliams, Kaylyn Renee. "In Vitro Models of Cellular Dedifferentiation for Regenerative Medicine." Thesis, Virginia Tech, 2018. http://hdl.handle.net/10919/83715.
Full textMaster of Science
Cui, Yixiao. "Recapitulating Brain Tumor Microenvironment with In Vitro Engineered Models." The Ohio State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu1595545538654859.
Full textSchjølberg, Tiril Helgesen. "In Vitro Synthesis of Metabolites of three Anabolic Androgenic Steroids, by Human Liver Microsomes." Thesis, Norges teknisk-naturvitenskapelige universitet, Institutt for bioteknologi, 2013. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-22910.
Full textWallace, Karen. "Glucocorticoid-mediated trans-differentiation of the pancreas to liver, in vitro and in vivo." Thesis, University of Newcastle Upon Tyne, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.512022.
Full text