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Journal articles on the topic 'In vitro; Embryology'

Author: Grafiati

Published: 4 June 2021

Last updated: 11 February 2022

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1

Zaninovic, Nikica, and Zev Rosenwaks. "Artificial intelligence in human in vitro fertilization and embryology." Fertility and Sterility 114, no. 5 (November 2020): 914–20. http://dx.doi.org/10.1016/j.fertnstert.2020.09.157.

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2

Opiela, J., and M. Samiec. "Characterization of mesenchymal stem cells and their application in experimental embryology." Polish Journal of Veterinary Sciences 16, no. 3 (September 1, 2013): 593–99. http://dx.doi.org/10.2478/pjvs-2013-0084.

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Abstract The efficiency of somatic cell cloning (somatic cell nuclear transfer; SCNT) as well as in vitro fertilization/in vitro embryo production (IVF/IVP) in mammals stay at relatively same level for over a decade. Despite plenty of different approaches none satisfactory break-through took place. In this article, we briefly summarize the implementation of mesenchymal stem cells (MSCs) for experimental embryology. The advantages of using MSCs as nuclear donors in somatic cell cloning and in vitro embryo culture are described. The description of results obtained with these cells in mammalian embryo genomic engineering is presented.

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3

Simopoulou, Mara, Konstantinos Sfakianoudis, Evangelos Maziotis, Anna Rapani, Polina Giannelou, Agni Pantou, George Anifandis, et al. "Assessing Clinical Embryology Research: A Global Bibliometric Analysis." Medicina 56, no. 5 (April 26, 2020): 210. http://dx.doi.org/10.3390/medicina56050210.

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Background and Objectives: The evaluative strength of available bibliometric tools in the field of clinical embryology has never been examined in the literature. The aim is to bring insight regarding the identity of clinical embryology research, introducing concerns when solely relying on the methodology of bibliometric analysis. Materials and Methods: An all-inclusive analysis of the most bibliometrically highlighted scientific contributions regarding the cornerstones of clinical embryology was performed employing the Scopus, Web of Science (WoS) and PubMed databases, between 1978–2018. An analysis of the number of publications, respective citations and h-index, g-index, along with m-quotient is presented. The top 30 contributing authors for each distinctive area of research are listed. An attempt at visualizing the yearly published articles, clusters, and collaborations of authors, along with the geographic origin of publications, is also presented. Results: Combining all searches and keywords yielded 54,522 results. In the Scopus database, employing the keyword “In Vitro Fertilization” yielded 41,292 results. The publications of the top five authors in each research field were analytically presented and compared to the total number of publications for each respective field. The research field of Preimplantation Genetic Diagnosis/Screening/Testing was allocated the highest percentage of publications produced by the top five authors. Regarding journal bibliometrics, based on the year 2017 metrics, there are only 29 journals according to WoS that refer to “Reproductive Biology”, ranking it 187th among 235 disciplines. The USA produced the highest number of publications (12,537). Conclusion: Results indicate an explosion of interest published in the literature regarding the field of clinical embryology. Further analysis on collaborations and the trends involved should be of added value as productivity between countries varies significantly. This may guide researchers, in vitro fertilization professionals, and prospective authors during literature search, while proving useful regarding manuscript design and concurring on keywords and abstract content.

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4

Ghaskadbi, Surendra. "Leela Mulherkar and the teaching of developmental biology." International Journal of Developmental Biology 64, no. 1-2-3 (2020): 41–44. http://dx.doi.org/10.1387/ijdb.200147sg.

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The formal teaching of developmental biology in India began in the late nineteen-fifties at the Department of Zoology of the University of Poona. This was due to the efforts of Leela Mulherkar, who on her return from C.H. Waddington’s laboratory in Edinburgh, took up the teaching of embryology at the Master’s level. Mulherkar began using locally available material to teach how animals develop. They included the embryos of chicken, frog, garden lizard and molluscs, as well as organisms such as hydra and sponges. Her teaching was supported by an active research laboratory that used all these systems to address a variety of questions in embryology and teratology. She used chick embryo explants cultured in vitro extensively in her work. Teaching and research in embryology at the master’s and doctoral levels at Poona University subsequently led, in 1977, to the establishment of the Indian Society of Developmental Biologists (InSDB), which is among the most active scientific societies in India.

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Coticchio, Giovanni, Mariabeatrice Dal-Canto, Maria-Cristina Guglielmo, Mario Mignini-Renzini, and Rubens Fadini. "Human oocyte maturation in vitro." International Journal of Developmental Biology 56, no. 10-11-12 (2012): 909–18. http://dx.doi.org/10.1387/ijdb.120135gv.

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6

Morales, P., V. palma, M. Salgado, and M. Villalon. "Fertilization and early embryology: Sperm interaction with human oviductal cells in vitro." Human Reproduction 11, no. 7 (July 1, 1996): 1504–9. http://dx.doi.org/10.1093/oxfordjournals.humrep.a019426.

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7

Plachot, Michelle, J. M. Antoine, Sylvia Alvarez, C. Firmin, A. Pfister, Jacqueline Mandelbaum, Anne-Marie Junca, and J. Salat-Baroux. "Fertilization and early embryology: Granulosa cells improve human embryo development in vitro." Human Reproduction 8, no. 12 (December 1993): 2133–40. http://dx.doi.org/10.1093/oxfordjournals.humrep.a137995.

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8

Krisher, Rebecca L., Adam L. Heuberger, Melissa Paczkowski, John Stevens, Courtney Pospisil, Randall S. Prather, Roger G. Sturmey, Jason R. Herrick, and William B. Schoolcraft. "Applying metabolomic analyses to the practice of embryology: physiology, development and assisted reproductive technology." Reproduction, Fertility and Development 27, no. 4 (2015): 602. http://dx.doi.org/10.1071/rd14359.

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The advent of metabolomics technology and its application to small samples has allowed us to non-invasively monitor the metabolic activity of embryos in a complex culture environment. The aim of this study was to apply metabolomics technology to the analysis of individual embryos from several species during in vitro development to gain an insight into the metabolomics pathways used by embryos and their relationship with embryo quality. Alanine is produced by both in vivo- and in vitro-derived human, murine, bovine and porcine embryos. Glutamine is also produced by the embryos of these four species, but only those produced in vitro. Across species, blastocysts significantly consumed amino acids from the culture medium, whereas glucose was not significantly taken up. There are significant differences in the metabolic profile of in vivo- compared with in vitro-produced embryos at the blastocyst stage. For example, in vitro-produced murine embryos consume arginine, asparagine, glutamate and proline, whereas in vivo-produced embryos do not. Human embryos produce more alanine, glutamate and glutamine, and consume less pyruvate, at the blastocyst compared with cleavage stages. Glucose was consumed by human blastocysts, but not at a high enough level to reach significance. Consumption of tyrosine by cleavage stage human embryos is indicative of blastocyst development, although tyrosine consumption is not predictive of blastocyst quality. Similarly, although in vivo-produced murine blastocysts consumed less aspartate, lactate, taurine and tyrosine than those produced in vitro, consumption of these four amino acids by in vitro-derived embryos with high octamer-binding transcription factor 4 (Oct4) expression, indicative of high quality, did not differ from those with low Oct4 expression. Further application of metabolomic technologies to studies of the consumption and/or production of metabolites from individual embryos in a complete culture medium could transform our understanding of embryo physiology and improve our ability to produce developmentally competent embryos in vitro.

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9

Sciorio, Romualdo, Erika Rapalini, and Sandro C. Esteves. "Air quality in the clinical embryology laboratory: a mini-review." Therapeutic Advances in Reproductive Health 15 (January 2021): 263349412199068. http://dx.doi.org/10.1177/2633494121990684.

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The scope of the clinical embryology laboratory has expanded over recent years. It now includes conventional in vitro fertilization (IVF) techniques and complex and time-demanding procedures like blastocyst culture, processing of surgically retrieved sperm, and trophectoderm biopsy for preimplantation genetic testing. These procedures require a stable culture environment in which ambient air quality might play a critical role. The existing data indicate that both particulate matter and chemical pollution adversely affect IVF results, with low levels for better outcomes. As a result, IVF clinics have invested in air cleaning technologies with variable efficiency to remove particulates and volatile organic compounds. However, specific regulatory frameworks mandating air quality control are limited, as are evidence-based guidelines for the best air quality control practices in the embryology laboratory. In this review, we describe the principles and existing solutions for improving air quality and summarize the clinical evidence concerning air quality control in the embryology laboratory. In addition, we discuss the gaps in knowledge that could guide future research to improve clinical outcomes.

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10

Ledger, William L. "Medicolegal aspects of reproductive medicine." Clinical Risk 15, no. 5 (September 2009): 197–200. http://dx.doi.org/10.1258/cr.2009.090061.

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This paper is a review of current techniques and best practice in reproductive medicine, including preimplantation genetic diagnosis and embryo freezing, and management of pregnancy after in vitro fertilization. It discusses medicolegal aspects that can arise from failure to follow best practice including ovarian hyperstimulation syndrome and mistakes occurring in the embryology laboratory.

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11

Lane, Michelle, Megan Mitchell, Kara S. Cashman, Deanne Feil, Sarah Wakefield, and Deirdre L. Zander-Fox. "To QC or not to QC: the key to a consistent laboratory?" Reproduction, Fertility and Development 20, no. 1 (2008): 23. http://dx.doi.org/10.1071/rd07161.

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A limiting factor in every embryology laboratory is its capacity to grow ‘normal’ embryos. In human in vitro fertilisation (IVF), there is considerable awareness that the environment of the laboratory itself can alter the quality of the embryos produced and the industry as a whole has moved towards the implementation of auditable quality management systems. Furthermore, in some countries, such as Australia, an established quality management system is mandatory for clinical IVF practice, but such systems are less frequently found in other embryology laboratories. Although the same challenges of supporting consistent and repeatable embryo development are paramount to success in all embryology laboratories, it could be argued that they are more important in a research setting where often the measured outcomes are at an intracellular or molecular level. In the present review, we have outlined the role and importance of quality control and quality assurance systems in any embryo laboratory and have highlighted examples of how simple monitoring can provide consistency and avoid the induction of artefacts, irrespective of the laboratory’s purpose, function or species involved.

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12

Turner, Christopher M., and Paul N. Adler. "Morphogenesis of Drosophila pupal wings in vitro." Mechanisms of Development 52, no. 2-3 (August 1995): 247–55. http://dx.doi.org/10.1016/0925-4773(95)00405-p.

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13

Kanakasabapathy, Manoj Kumar, Prudhvi Thirumalaraju, Charles L. Bormann, Hemanth Kandula, Irene Dimitriadis, Irene Souter, Vinish Yogesh, et al. "Development and evaluation of inexpensive automated deep learning-based imaging systems for embryology." Lab on a Chip 19, no. 24 (2019): 4139–45. http://dx.doi.org/10.1039/c9lc00721k.

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14

Doyle, Pat. "THE U.K. HUMAN FERTILISATION AND EMBRYOLOGY AUTHORITY." International Journal of Technology Assessment in Health Care 15, no. 1 (January 1999): 3–10. http://dx.doi.org/10.1017/s0266462399015123.

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The Human Fertilisation and Embryology Act of the United Kingdom was passed in 1990, leading to the formation of the Human Fertilisation and Embryology Authority (HFEA), the first statutory body to regulate and control assisted conception anywhere in the world. The principal function of the HFEA is to license and monitor clinics that carry out in vitro fertilization (IVF), donor insemination (DI), and embryo research. Information on over 135,000 treatment cycles, 20,000 pregnancies, and 25,000 babies following IVF has now been collected as part of the regulatory process, and these data have permitted unbiased and accurate evaluation of treatment efficacy using pregnancy and live-birth rates. The treating clinics are required by law to provide information on the outcome of all births, including neonatal mortality and congenital malformations, but there is no systematic validation of these data using medical records or any follow-up of treated women, or babies, over time. In addition, the strict confidentiality of data supplied to the HFEA means that outside researchers have been unable to access the database for research projects. Thus, at the present time, it is not possible to evaluate the long-term safety of assisted conception procedures using HFEA data. There is reasonable scientific evidence to justify full investigation of the health of both treated women and resulting children. Particular health outcomes requiring evaluation include obstetric complications, preterm births, cerebral palsy, and cancer. The HFEA has recognized the need for follow-up studies and is currently investigating ways of enabling research projects using HFEA data to be undertaken.

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15

Telfer, Evelyn E., and Marie Mclaughlin. "Strategies to support human oocyte development in vitro." International Journal of Developmental Biology 56, no. 10-11-12 (2012): 901–7. http://dx.doi.org/10.1387/ijdb.130001et.

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16

Asashima, Makoto. "S08-07 In vitro organogenesis in vertebrate development." Mechanisms of Development 126 (August 2009): S33—S34. http://dx.doi.org/10.1016/j.mod.2009.06.1045.

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17

Greve, Torben, and Henrik Callesen. "Integrating new technologies with embryology and animal production." Reproduction, Fertility and Development 16, no. 2 (2004): 113. http://dx.doi.org/10.1071/rd03084.

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The present review describes a range of selected farm animal embryo technologies used in embryological research and applied in animal breeding and production. Some of the techniques are driven by the breeder’s wish to obtain animals with higher breeding values, whereas others are primarily driven by the curiosity of researchers. The interaction between basic research and practical application in these areas is still a characteristic feature for people who contribute to the International Embryo Transfer Society (IETS) and has been an advantage for both researchers and breeders. One example of such an interaction is that detailed structural analyses have described quality differences between embryos of various origins and, following embryo transfer, the pregnancy results have confirmed the correlation between morphology and viability. Another example is that polymerase chain reaction technology has allowed detection of Y-specific sequences in male embryos and has become a tool in animal production today. Data from domestic animal genome sequencing will provide a great deal of new information. A major challenge for the years to come will be using this information in a physiologically meaningful context and to continue the efforts to convert the laboratory experience into use in practise. Finally, it is important to obtain societal acceptance for a wider application of many of the technologies, such as in vitro embryo production and cloning.

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18

Lasala, Gina, Donatella Farini, and Massimo De Felici. "Estrogenic in vitro assay on mouse embryonic Leydig cells." International Journal of Developmental Biology 54, no. 4 (2010): 717–22. http://dx.doi.org/10.1387/ijdb.092883gs.

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19

Hyttel, P., K. P. Xu, and T. Greve. "Ultrastructural abnormalities of in vitro fertilization of in vitro matured bovine oocytes." Anatomy and Embryology 178, no. 1 (1988): 47–52. http://dx.doi.org/10.1007/bf00305013.

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20

Vityazeva, I. I., I. I. Barmina, and G. A. Melnichenko. "Historical stages of development of methods of the assisted reproductive technologies based on fertilisation in vitro." Bulletin of Reproductive Health, no. 1 (March 17, 2011): 5–14. http://dx.doi.org/10.14341/brh201115-14.

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This article is devoted, in the first place, to the history of in vitro fertilization method (IVF). The main phase of initiation and further perfection of IVF are elucidated. Correlations of the development knowledge and skills in obstetrics and gynecology, endocrinology, pharmacology are revealed. In a chronological order the main achievements of such reproduction medicine sections as embryology with cryopreservation and PGD analysis, functional diagnostics with ultrasonography are recited. Further practical and scientific perspectives of the artificial reproductive technology's method are determined. Special attention attends to the IVF history in Russia and ART problem's condition at present.

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21

Li, Wan-Chun, Marko E. Horb, David Tosh, and Jonathan M. W. Slack. "In vitro transdifferentiation of hepatoma cells into functional pancreatic cells." Mechanisms of Development 122, no. 6 (June 2005): 835–47. http://dx.doi.org/10.1016/j.mod.2005.01.001.

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22

Miyagoe-Suzuki, Yuko, Nami Masubuchi, Kaori Miyamoto, Michiko R. Wada, Shigeki Yuasa, Fumiaki Saito, Kiichiro Matsumura, et al. "Reduced proliferative activity of primary POMGnT1-null myoblasts in vitro." Mechanisms of Development 126, no. 3-4 (March 2009): 107–16. http://dx.doi.org/10.1016/j.mod.2008.12.001.

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23

Qiao, Jizeng, Anya Turetsky, Paul Kemp, and Jeff Teumer. "Hair morphogenesisin vitro: formation of hair structures suitable for implantation." Regenerative Medicine 3, no. 5 (September 2008): 683–92. http://dx.doi.org/10.2217/17460751.3.5.683.

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24

Iacob, Razvan, Vlad Herlea, Lorand Savu, Ioana R Florea, Veronica M Ilie, George Terinte-Balcan, Mihaela Gherghiceanu, et al. "Phenotypic assessment of liver-derived cell cultures during in vitro expansion." Regenerative Medicine 16, no. 1 (January 2021): 33–46. http://dx.doi.org/10.2217/rme-2020-0093.

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Background: Liver cells represent an attractive source of cells for autologous regenerative medicine. The present study assesses the liver cells’ stability during in vitro expansion, as a prerequisite for therapeutic use. Results: The human liver cell cultures in this study were propagated efficiently in vitro for at least 12 passages. No significant changes in morphology, intracellular ultrastructures and characteristic markers expression were found during in vitro expansion of cells from all analyzed donors. However, expanded cells derived from male donors of >60 years old, lost the Y chromosome. Conclusion: Liver-derived cell cultures adopt a proliferative, stable mesenchymal phenotype, through an epithelial to mesenchymal transition process. The molecular and phenotypic changes of the cells during propagation are uniform, despite the heterogeneity of the different donors. Loss of Y chromosome occurs after cells’ propagation in elder male donors.

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25

Dumoulin, John C. M., Anton H. J. C. Michiels, Marijke Bras, Math H. E. C. Pieters, Joep P. M. Geraedts, and Johannes L. H. Evers. "Fertilization and early embryology: Temporal effects of ouabain on in-vitro development of mouse zygotes." Human Reproduction 8, no. 9 (September 1993): 1469–74. http://dx.doi.org/10.1093/oxfordjournals.humrep.a138281.

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26

Tournaye, Herman, Marleen Van der Linden, Etienne Van den Abbeel, Paul Devroey, and Andre Van Steirteghem. "Fertilization and early embryology: Effects of pentoxifylline on in-vitro development of preimplantation mouse embryos." Human Reproduction 8, no. 9 (September 1993): 1475–80. http://dx.doi.org/10.1093/oxfordjournals.humrep.a138282.

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27

Aboulghar, M. A., R. T. Mansour, G. I. Serour, and Y. M. Amin. "Fertilization and early embryology: The prognostic value of successful in-vitro fertilization in subsequent trials." Human Reproduction 9, no. 10 (October 1994): 1932–34. http://dx.doi.org/10.1093/oxfordjournals.humrep.a138361.

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28

Rooney, GE, GI Nistor, FB Barry, and HS Keirstead. "In vitro differentiation potential of human embryonic versus adult stem cells." Regenerative Medicine 5, no. 3 (May 2010): 365–79. http://dx.doi.org/10.2217/rme.10.20.

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29

Kalionis, Bill, and Patrick H. O'Farrell. "A universal target sequence is bound in vitro by diverse homeodomains." Mechanisms of Development 43, no. 1 (September 1993): 57–70. http://dx.doi.org/10.1016/0925-4773(93)90023-q.

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30

Yeung, Queenie S. Y., Ying Xin Zhang, Jacqueline P. W. Chung, Yvonne K. Y. Kwok, Baoheng Gui, Kwong Wai Choy, and Tin Chiu Li. "Practical Considerations in Providing Preimplantation Genetic Testing for Aneuploidies (PGT-A)." Fertility & Reproduction 01, no. 01 (March 2019): 21–29. http://dx.doi.org/10.1142/s2661318219300046.

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Preimplantation genetic testing for aneuploidies (PGT-A) has been controversial in its application to improve reproductive success, reduce time-to-pregnancy, and serve the intention-to-treat. Nevertheless, many in vitro fertilization (IVF) units have already introduced the service for one reason or another. Given PGT-A is not a stand-alone technique but a clinical service involving several disciplines, this mini review discussed the factors that can influence success rates when PGT-A is applied and highlighted practical issues encountered by clinicians, embryology, and genetics laboratories involved in the provision of PGT-A service.

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31

Nieto-Nicolau, Núria, Beatriz Martín-Antonio, Claudia Müller-Sánchez, and Ricardo P. Casaroli-Marano. "In vitro potential of human mesenchymal stem cells for corneal epithelial regeneration." Regenerative Medicine 15, no. 3 (March 2020): 1409–26. http://dx.doi.org/10.2217/rme-2019-0067.

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Aim: To determine the potential of mesenchymal stem cells (MSC) for corneal epithelial regeneration in vitro. Materials & methods: Bone marrow MSC (BM-MSC) and adipose tissue MSC were analyzed for corneal epithelial and mesenchymal markers, using limbal stem cells and corneal cells as controls. MSC with better potential were cultured with specific mediums for epithelial induction. Transepithelial electric resistance and wound healing assay with human corneal epithelial cells were performed. Results: BM-MSC showed better potential, increased corneal markers, and higher transepithelial electric resistance values when induced with limbal epithelial culture medium. Induced BM-MSC promoted better wound healing of human corneal epithelial cells by paracrine secretion. Conclusion: BM-MSC has potential for corneal epithelial induction in a protocol compatible with human application.

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Song, Renfang, Graeme Preston, and Ihor V. Yosypiv. "Angiotensin II stimulates in vitro branching morphogenesis of the isolated ureteric bud." Mechanisms of Development 128, no. 7-10 (September 2011): 359–67. http://dx.doi.org/10.1016/j.mod.2011.07.002.

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33

Eguizabal, Cristina, Maria D. Boyano, Alejandro Diez-Torre, Ricardo Andrade, Noelia Andollo, Massimo De Felici, and Juan Arechaga. "Interleukin-2 induces the proliferation of mouse primordial germ cells in vitro." International Journal of Developmental Biology 51, no. 8 (2007): 731–38. http://dx.doi.org/10.1387/ijdb.072442ce.

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34

Honda, Arata, Michiko Hirose, Kimiko Inoue, Hitoshi Hiura, Hiromi Miki, Narumi Ogonuki, Michihiko Sugimoto, et al. "Large-scale production of growing oocytes in vitro from neonatal mouse ovaries." International Journal of Developmental Biology 53, no. 4 (2009): 605–13. http://dx.doi.org/10.1387/ijdb.082607ah.

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35

Kobayashi, Tohru. "In vitro germ cell differentiation during sex differentiation in a teleost fish." International Journal of Developmental Biology 54, no. 1 (2010): 105–11. http://dx.doi.org/10.1387/ijdb.082836tk.

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36

Hyttel, P. "Bovine cumulus-oocyte disconnection in vitro." Anatomy and Embryology 176, no. 1 (April 1987): 41–44. http://dx.doi.org/10.1007/bf00309750.

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37

Baldwin, R. "Book Review: Fourth Report of the Voluntary Licensing Authority for Human In Vitro Fertilisation and Embryology." Medicine, Science and the Law 30, no. 2 (April 1990): 183. http://dx.doi.org/10.1177/002580249003000220.

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38

Hyodo, Masao, Shinji Makino, Yasunori Awaji, Yohei Sakurada, Tomoichi Ohkubo, Mitsushige Murata, Keiichi Fukuda, and Michio Tsuda. "A novel in vitro system for studying cardiomyocyte differentiation with medaka embryonic cells." International Journal of Developmental Biology 53, no. 4 (2009): 615–22. http://dx.doi.org/10.1387/ijdb.092850mh.

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39

Choi, Soon Won, Sigrid Eckardt, Ruhel Ahmad, Wanja Wolber, K. John McLaughlin, Anna-Leena Siren, and Albrecht M. Mller. "Two paternal genomes are compatible with dopaminergic in vitro and in vivo differentiation." International Journal of Developmental Biology 54, no. 11-12 (2010): 1755–62. http://dx.doi.org/10.1387/ijdb.103188sc.

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40

Govindasamy, Niraimathi, Binyamin Duethorn, Hatice O. Oezgueldez, Yung S. Kim, and Ivan Bedzhov. "Test-tube embryos - mouse and human development in vitro to blastocyst stage and beyond." International Journal of Developmental Biology 63, no. 3-4-5 (2019): 203–15. http://dx.doi.org/10.1387/ijdb.180379ib.

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Mammalian embryogenesis is intrauterine and depends on support from the maternal environment. Therefore, in order to directly study and manipulate early mouse and human embryos, fine-tuned culture conditions have to be provided to maintain embryo growth in vitro. Over time, the establishment and implementation of embryo culture methods have come a long way, initially enabling the development of few pre-implantation stages, expanding later to support in vitro embryogenesis from fertilization until blastocyst and even ex utero development beyond the implantation stages. Designing culture conditions that enable near physiological development of early embryos without maternal input, especially during the peri- and post-implantation stages, requires overcoming numerous experimental challenges, and it is still far from optimal. Nevertheless, embryo culture methods are an essential cornerstone of both assisted reproductive technologies and basic research, and these methods provide a platform to understand life’s greatest miracle – the development of a new organism.

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41

Li, Yan, Chunhui Xu, and Teng Ma. "In vitro organogenesis from pluripotent stem cells." Organogenesis 10, no. 2 (April 2014): 159–63. http://dx.doi.org/10.4161/org.28918.

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42

Ataliotis, Paris. "Platelet-derived growth factor A modulates limb chondrogenesis both in vivo and in vitro." Mechanisms of Development 94, no. 1-2 (June 2000): 13–24. http://dx.doi.org/10.1016/s0925-4773(00)00321-x.

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43

Renoncourt, Yannick, Patrick Carroll, Pierre Filippi, Vilma Arce, and Serge Alonso. "Neurons derived in vitro from ES cells express homeoproteins characteristic of motoneurons and interneurons." Mechanisms of Development 79, no. 1-2 (December 1998): 185–97. http://dx.doi.org/10.1016/s0925-4773(98)00189-0.

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44

Archacka, Karolina, Anna Ajduk, Pawel Pomorski, Katarzyna Szczepanska, Marek Maleszewski, and Maria A. Ciemerych. "Defective calcium release during in vitro fertilization of maturing oocytes of LT/Sv mice." International Journal of Developmental Biology 52, no. 7 (2008): 903–12. http://dx.doi.org/10.1387/ijdb.072397ka.

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45

Ito, Shoko, and Masatoshi Takeichi. "12-P015 In vitro recognition of specific afferent axons by cerebellar granule cell dendrites." Mechanisms of Development 126 (August 2009): S193. http://dx.doi.org/10.1016/j.mod.2009.06.469.

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Bouwmeester, Tewis, Stephan Güehmann, Tarek El-Baradi, Frank Kalkbrenner, Inge van Wijk, Karin Moelling, and Tomas Pieler. "Molecular cloning, expression and in vitro functional characterization of Myb-related proteins in Xenopus." Mechanisms of Development 37, no. 1-2 (March 1992): 57–68. http://dx.doi.org/10.1016/0925-4773(92)90015-c.

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Ashfaq, Ramla, Azra Mehmood, Amna Ramzan, Intzar Hussain, Moazzam Nazeer Tarar, and Sheikh Riazuddin. "Antioxidant pretreatment enhances umbilical cord derived stem cells survival in response to thermal stress in vitro." Regenerative Medicine 15, no. 3 (March 2020): 1441–53. http://dx.doi.org/10.2217/rme-2019-0090.

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Aim: Pretreatment of stem cells with antioxidants accelerates their ability to counter oxidative stress and is associated with the overall therapeutic outcome of their transplantation. Material & methods: Wharton Jelly derived mesenchymal stem cells (WJMSCs) were cultured and pretreated with various doses of antioxidants; Vitamin C (Vit C), Vitamin E (Vit E), Vitamin D3 (Vit D3) and their Cocktail, followed by exposure to in vitro heat injury. Assessment of WJMSCs survival, paracrine release, in vitro wound healing and expression of angiogenic and survival markers was conducted. Results: The results displayed an enhanced survival of WJMSCs especially in the case of Cocktail priming. Conclusion: Our data suggest that antioxidant pretreatment of WJMSCs strengthens the endurance of the cells, within stress conditions.

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Tournaye, Herman, Marleen Van der Linden, Etienne Van den Abbeel, Paul Devroey, and André Van Steirteghem. "Fertilization and early embryology: The effect of pentoxifylline on mouse in-vitro fertilization and early embryonic development." Human Reproduction 9, no. 10 (October 1994): 1903–8. http://dx.doi.org/10.1093/oxfordjournals.humrep.a138356.

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Yao, Y. Q., W. S. B. Yeung, and P. C. Ho. "Fertilization and early embryology: Human follicular fluid inhibits the binding of human spermatozoa to zona pellucidain vitro." Human Reproduction 11, no. 12 (December 1, 1996): 2674–80. http://dx.doi.org/10.1093/oxfordjournals.humrep.a019190.

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S Zaitseva, Tatiana, Guang Yang, Dimitris Dionyssiou, Maedeh Zamani, Steve Sawamura, Eduard Yakubov, James Ferguson, et al. "Delivery of hepatocyte growth factor mRNA from nanofibrillar scaffolds in a pig model of peripheral arterial disease." Regenerative Medicine 15, no. 6 (June 2020): 1761–73. http://dx.doi.org/10.2217/rme-2020-0023.

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Background: Chemical modification of mRNA (mmRNA) substantially improves their stability and translational efficiency within cells. Nanofibrillar collagen scaffolds were previously shown to enable the spatially localized delivery and temporally controlled release of mmRNA encoding HGF both in vitro and in vivo. Materials & methods: Herein we developed an improved slow-releasing HGF mmRNA scaffold and tested its therapeutic efficacy in a porcine model of peripheral arterial disease. Results & conclusion: The HGF mmRNA was released from scaffolds in a temporally controlled fashion in vitro with preserved transfection activity. The mmRNA scaffolds improved vascular regeneration when sutured to the ligated porcine femoral artery. These studies validate the therapeutic potential of HGF mmRNA delivery from nanofibrillar scaffolds for treatment of peripheral arterial disease.

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