Academic literature on the topic 'In vitro; Embryology'

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Journal articles on the topic "In vitro; Embryology"

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Zaninovic, Nikica, and Zev Rosenwaks. "Artificial intelligence in human in vitro fertilization and embryology." Fertility and Sterility 114, no. 5 (November 2020): 914–20. http://dx.doi.org/10.1016/j.fertnstert.2020.09.157.

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Opiela, J., and M. Samiec. "Characterization of mesenchymal stem cells and their application in experimental embryology." Polish Journal of Veterinary Sciences 16, no. 3 (September 1, 2013): 593–99. http://dx.doi.org/10.2478/pjvs-2013-0084.

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Abstract The efficiency of somatic cell cloning (somatic cell nuclear transfer; SCNT) as well as in vitro fertilization/in vitro embryo production (IVF/IVP) in mammals stay at relatively same level for over a decade. Despite plenty of different approaches none satisfactory break-through took place. In this article, we briefly summarize the implementation of mesenchymal stem cells (MSCs) for experimental embryology. The advantages of using MSCs as nuclear donors in somatic cell cloning and in vitro embryo culture are described. The description of results obtained with these cells in mammalian embryo genomic engineering is presented.
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Simopoulou, Mara, Konstantinos Sfakianoudis, Evangelos Maziotis, Anna Rapani, Polina Giannelou, Agni Pantou, George Anifandis, et al. "Assessing Clinical Embryology Research: A Global Bibliometric Analysis." Medicina 56, no. 5 (April 26, 2020): 210. http://dx.doi.org/10.3390/medicina56050210.

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Background and Objectives: The evaluative strength of available bibliometric tools in the field of clinical embryology has never been examined in the literature. The aim is to bring insight regarding the identity of clinical embryology research, introducing concerns when solely relying on the methodology of bibliometric analysis. Materials and Methods: An all-inclusive analysis of the most bibliometrically highlighted scientific contributions regarding the cornerstones of clinical embryology was performed employing the Scopus, Web of Science (WoS) and PubMed databases, between 1978–2018. An analysis of the number of publications, respective citations and h-index, g-index, along with m-quotient is presented. The top 30 contributing authors for each distinctive area of research are listed. An attempt at visualizing the yearly published articles, clusters, and collaborations of authors, along with the geographic origin of publications, is also presented. Results: Combining all searches and keywords yielded 54,522 results. In the Scopus database, employing the keyword “In Vitro Fertilization” yielded 41,292 results. The publications of the top five authors in each research field were analytically presented and compared to the total number of publications for each respective field. The research field of Preimplantation Genetic Diagnosis/Screening/Testing was allocated the highest percentage of publications produced by the top five authors. Regarding journal bibliometrics, based on the year 2017 metrics, there are only 29 journals according to WoS that refer to “Reproductive Biology”, ranking it 187th among 235 disciplines. The USA produced the highest number of publications (12,537). Conclusion: Results indicate an explosion of interest published in the literature regarding the field of clinical embryology. Further analysis on collaborations and the trends involved should be of added value as productivity between countries varies significantly. This may guide researchers, in vitro fertilization professionals, and prospective authors during literature search, while proving useful regarding manuscript design and concurring on keywords and abstract content.
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Ghaskadbi, Surendra. "Leela Mulherkar and the teaching of developmental biology." International Journal of Developmental Biology 64, no. 1-2-3 (2020): 41–44. http://dx.doi.org/10.1387/ijdb.200147sg.

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The formal teaching of developmental biology in India began in the late nineteen-fifties at the Department of Zoology of the University of Poona. This was due to the efforts of Leela Mulherkar, who on her return from C.H. Waddington’s laboratory in Edinburgh, took up the teaching of embryology at the Master’s level. Mulherkar began using locally available material to teach how animals develop. They included the embryos of chicken, frog, garden lizard and molluscs, as well as organisms such as hydra and sponges. Her teaching was supported by an active research laboratory that used all these systems to address a variety of questions in embryology and teratology. She used chick embryo explants cultured in vitro extensively in her work. Teaching and research in embryology at the master’s and doctoral levels at Poona University subsequently led, in 1977, to the establishment of the Indian Society of Developmental Biologists (InSDB), which is among the most active scientific societies in India.
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Coticchio, Giovanni, Mariabeatrice Dal-Canto, Maria-Cristina Guglielmo, Mario Mignini-Renzini, and Rubens Fadini. "Human oocyte maturation in vitro." International Journal of Developmental Biology 56, no. 10-11-12 (2012): 909–18. http://dx.doi.org/10.1387/ijdb.120135gv.

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Morales, P., V. palma, M. Salgado, and M. Villalon. "Fertilization and early embryology: Sperm interaction with human oviductal cells in vitro." Human Reproduction 11, no. 7 (July 1, 1996): 1504–9. http://dx.doi.org/10.1093/oxfordjournals.humrep.a019426.

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Plachot, Michelle, J. M. Antoine, Sylvia Alvarez, C. Firmin, A. Pfister, Jacqueline Mandelbaum, Anne-Marie Junca, and J. Salat-Baroux. "Fertilization and early embryology: Granulosa cells improve human embryo development in vitro." Human Reproduction 8, no. 12 (December 1993): 2133–40. http://dx.doi.org/10.1093/oxfordjournals.humrep.a137995.

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Krisher, Rebecca L., Adam L. Heuberger, Melissa Paczkowski, John Stevens, Courtney Pospisil, Randall S. Prather, Roger G. Sturmey, Jason R. Herrick, and William B. Schoolcraft. "Applying metabolomic analyses to the practice of embryology: physiology, development and assisted reproductive technology." Reproduction, Fertility and Development 27, no. 4 (2015): 602. http://dx.doi.org/10.1071/rd14359.

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The advent of metabolomics technology and its application to small samples has allowed us to non-invasively monitor the metabolic activity of embryos in a complex culture environment. The aim of this study was to apply metabolomics technology to the analysis of individual embryos from several species during in vitro development to gain an insight into the metabolomics pathways used by embryos and their relationship with embryo quality. Alanine is produced by both in vivo- and in vitro-derived human, murine, bovine and porcine embryos. Glutamine is also produced by the embryos of these four species, but only those produced in vitro. Across species, blastocysts significantly consumed amino acids from the culture medium, whereas glucose was not significantly taken up. There are significant differences in the metabolic profile of in vivo- compared with in vitro-produced embryos at the blastocyst stage. For example, in vitro-produced murine embryos consume arginine, asparagine, glutamate and proline, whereas in vivo-produced embryos do not. Human embryos produce more alanine, glutamate and glutamine, and consume less pyruvate, at the blastocyst compared with cleavage stages. Glucose was consumed by human blastocysts, but not at a high enough level to reach significance. Consumption of tyrosine by cleavage stage human embryos is indicative of blastocyst development, although tyrosine consumption is not predictive of blastocyst quality. Similarly, although in vivo-produced murine blastocysts consumed less aspartate, lactate, taurine and tyrosine than those produced in vitro, consumption of these four amino acids by in vitro-derived embryos with high octamer-binding transcription factor 4 (Oct4) expression, indicative of high quality, did not differ from those with low Oct4 expression. Further application of metabolomic technologies to studies of the consumption and/or production of metabolites from individual embryos in a complete culture medium could transform our understanding of embryo physiology and improve our ability to produce developmentally competent embryos in vitro.
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Sciorio, Romualdo, Erika Rapalini, and Sandro C. Esteves. "Air quality in the clinical embryology laboratory: a mini-review." Therapeutic Advances in Reproductive Health 15 (January 2021): 263349412199068. http://dx.doi.org/10.1177/2633494121990684.

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The scope of the clinical embryology laboratory has expanded over recent years. It now includes conventional in vitro fertilization (IVF) techniques and complex and time-demanding procedures like blastocyst culture, processing of surgically retrieved sperm, and trophectoderm biopsy for preimplantation genetic testing. These procedures require a stable culture environment in which ambient air quality might play a critical role. The existing data indicate that both particulate matter and chemical pollution adversely affect IVF results, with low levels for better outcomes. As a result, IVF clinics have invested in air cleaning technologies with variable efficiency to remove particulates and volatile organic compounds. However, specific regulatory frameworks mandating air quality control are limited, as are evidence-based guidelines for the best air quality control practices in the embryology laboratory. In this review, we describe the principles and existing solutions for improving air quality and summarize the clinical evidence concerning air quality control in the embryology laboratory. In addition, we discuss the gaps in knowledge that could guide future research to improve clinical outcomes.
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Ledger, William L. "Medicolegal aspects of reproductive medicine." Clinical Risk 15, no. 5 (September 2009): 197–200. http://dx.doi.org/10.1258/cr.2009.090061.

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This paper is a review of current techniques and best practice in reproductive medicine, including preimplantation genetic diagnosis and embryo freezing, and management of pregnancy after in vitro fertilization. It discusses medicolegal aspects that can arise from failure to follow best practice including ovarian hyperstimulation syndrome and mistakes occurring in the embryology laboratory.
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Dissertations / Theses on the topic "In vitro; Embryology"

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Uy, Gary D. "Studies on the control of diploid trophoblast growth in the mouse." Thesis, University of Oxford, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.365366.

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Nasr-Esfahani, Mohammad Hossein. "The role of reactive oxygen species in the vitro development of mouse embryos." Thesis, University of Cambridge, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.387105.

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Xu, Jiasen. "A study of embryotrophic mechanism of human oviductal cells on mouse embryo development in vitro." Hong Kong : University of Hong Kong, 2000. http://sunzi.lib.hku.hk/hkuto/record.jsp?B22926197.

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Chan, Sin-ying Cindy. "In vitro effect of oviductal embryotrophic factors on the gene expressions of preimplantation mouse embryos." Click to view the E-thesis via HKUTO, 2003. http://sunzi.lib.hku.hk/hkuto/record/B31970916.

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Black, David H. "Development of ovum pickup and in vitro embryo production to assess fertility responses for mineral intervention studies." Thesis, University of Nottingham, 2018. http://eprints.nottingham.ac.uk/52598/.

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As nutrition is of central importance to cattle fertility, this study sought to assess how veterinarians and nutritional advisers manage trace element imbalance in the UK; diagnosis and treatment. The study also sought to develop a robust system for oocyte recovery (ovum pick-up (OPU)) and in vitro embryo production (IVP) for commercial use, and to identify key factors influencing success, including oestrus synchrony and ovarian stimulation prior to OPU. The intention originally was to use OPU/IVP to investigate the impact of mineral imbalances on bovine oocyte quality, early embryo development and pregnancy establishment following embryo transfer (ET). In the first survey of its kind in the UK, the understanding and approach of advisers to mineral nutrition on farms was investigated. Of the 173 respondents, 78% were vets in practice. The overall importance of minerals was recorded by vets as low 33%, medium 37%, and high 30%, while non-vets scored importance as low 17%, medium 48%, and high 35%. There was little consensus amongst the advisers, or within the vet and non-vet subgroups about mechanisms and interactions associated with deficiency, and particularly of copper responsive conditions. The most frequently identified deficiencies were selenium, copper and iodine, while the most commonly identified toxicity was molybdenum. For copper responsive conditions, all of the listed treatments were used at least "occasionally"; the most frequently being glass boluses, in-feed supplementation, matrix boluses, and then copper injections. While there was a diverse choice of treatments, altering the ration was relatively rarely selected. This thesis also provides the first large-scale retrospective analysis of factors influencing the establishment of a commercially robust ovum pick up (OPU) and in vitro embryo production (IVP) platform in the UK. Over a 5-year period, a system was developed and validated for use in the UK with 2,138 cycles of OPU. These cycles were analysed as four sets of data and included two IVP laboratories and 6 OPU teams. Factors in these analyses included OPU team, IVP laboratory, ovarian stimulation protocol and semen type (unsorted vs sex-sorted). The mean number of follicles aspirated by the OPU teams ranged from 6.5 to 14.9 (P < 0.001), while the number of oocytes collected was between 4.0 and 12.4 (P < 0.001). There was an indication (P=0.055) that the blastocyst per oocyte rate varied between teams. The proportion of blastocysts from oocytes that cleaved was higher (P=0.01) for unsorted than sexed semen. Two commercial products containing different ratios of follicle stimulating hormone (FSH) to luteinising hormone (LH) (Folltropin® and Pluset®) were compared in ovarian stimulation programs. The addition of 'coasting' (short-term (typically 48h) hormonal withdrawal after FSH stimulation), prior to OPU was also investigated. Pluset® resulted in a greater (P < 0.001) mean number of follicles aspirated, more (P=0.003) blastocysts per oocyte matured and more (P < 0.001) embryos per cycle (2.45), compared with Folltropin® (1.17) or with no stimulation (1.24). Throughout the study there was a steady improvement in blastocyst production per OPU cycle. In a separate analysis, Grade 1 cumulus oocyte complexes (COCs) as a proportion of COCs recovered, oocytes that cleaved as a proportion of total COCs, and blastocysts as a proportion of total COCs, were all greater (P < 0.05) for stimulated than non-stimulated cycles, irrespective of FSH/LH product. A composite score of oocyte quality and quantity was proposed (sCOC); Log Total Mean sCOC was correlated (P < 0.001) with both the proportion of blastocysts per oocyte collected, and the total number of embryos produced per cycle. Finally, twelve peri-pubertal heifers (approximately 10 months old) participated in a crossover trial which compared PRID® (Delta®) vs CIDR® progesterone releasing intravaginal devices for use in OPU/IVP cycles. Vaginoscopic examination found higher vaginal inflammation grades for PRID® than CIDR® (P < 0.001). There was evidence of vaginal inflammation continuing for at least 2 weeks after device withdrawal. The proportion cleaved of oocytes inseminated was higher for PRID® than CIDR® (P < 0.05). Numerically but not significantly there was a higher proportion of blastocysts per cycle and a higher Log Total Mean sCOC score per cycle with PRID® than CIDR® treatments, but blastocyst yield was low throughout, suggesting a need to repeat the trial. Data collection and analyses are ongoing, to identify other key performance indicators within the OPU/IVP embryo transfer (ET) system, with a view to refining the sCOC composite score model. A robust OPU/IVP/ET system has been developed and this could be used to investigate further how mineral imbalances impact oocyte competence and blastocyst yield.
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陳倩瑩 and Sin-ying Cindy Chan. "In vitro effect of oviductal embryotrophic factors on the gene expressions of preimplantation mouse embryos." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B31970916.

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Yu, Hing-Sing. "Studies on the toxicity and teratogenicity of cadmium on mouse pre-embryos in vitro and in vivo with special reference to their subsequent development /." [Hong Kong] : University of Hong Kong, 1987. http://sunzi.lib.hku.hk/hkuto/record.jsp?B1221579X.

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許嘉森 and Jiasen Xu. "A study of embryotrophic mechanism of human oviductal cells on mouse embryo development in vitro." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2000. http://hub.hku.hk/bib/B43894720.

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Lawry, John R. "Characterization of the expression of glutamate dehydrogenase in preimplantation mouse embryos using competitive reverse transcription- polymerase chain reactions." Virtual Press, 1994. http://liblink.bsu.edu/uhtbin/catkey/917022.

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A mouse embryo culture medium which would allow for in vitro development from 1-cell stage to blastocyst stage could offer many benefits for human research. Previous researchfrom our lab has demonstrated a mouse embryo culture medium named CZB seems to allow for in vivo-like conditions for development. Compared to other commonly used mouse embryo culture media, CZB medium promotes a higher frequency of 1 cell mouse embryos developing to blastocyst stage (Chatot et 1989). A key difference between CZB and other mouse embryo culture media is that CZB contains the amino acid glutamine metabolism is glutamate dehydrogenase (GDH). In order to determine if CZB cultured embryos follow in vivo-like patterns of gene expression for GDH, a quantitative competitive RT-PCR system was designed. A mutant GDH mRNA template was created which lacked a specific restriction enzyme site and was used as a competitive template in quantitative RT-PCR. This system was used to determine the amount of GDH mRNA present in in vivo grown blastocyst stage mouse embryos. It was determined that the amount of GDH mRNA present in in vivo blastocyst stage embryos was 282 fg/embryo. It is believed this system will also allow for quantitation of GDH mRNA in the earlier preimplantation stages of in vivo grown embryos, as well as the preimplantation stages 2-cell to blastocyst of CZB cultured embryos.
Department of Biology
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Sedano, Rodolfo Canseco. "Effect of immunoglobulins on early bovine embryo development in vitro." Thesis, Virginia Tech, 1985. http://hdl.handle.net/10919/41575.

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Bovine morulae (day 6: n=257) were obtained to evaluate the [effect of immunoglobulins (Ig) on early bovine embryo development in vitro. Fifty-four cows superovulations were conducted in 36 cows with follicle stimulating hormone. Embryos were collected by non-surgical procedures and morphologically evaluated and randomly assigned to culture. Embryos were cultured in Ham's F-10 containing 10% (6.4 mg/ml) steer serum (SS), 1% (.64 mg/ml) bovine gamma . globulins (GG), 1% (.64 mg/ml) bovine IgG, 1% (.64 mg/ml) bovine 1gM, 10% SS plus 1% GG, 10% SS plus 1% 1gG, or 10% SS plus 1% 1gM. Embryos were cultured to the hatched blastocyst stage or degeneration and evaluated at 12 h intervals.
Master of Science
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Books on the topic "In vitro; Embryology"

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Birk, Dewinder. Human fertilisation and embryology: The new law. Bristol, England: Family Law, 2009.

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Human fertilisation and embryology: The new law. Bristol, England: Family Law, 2009.

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Jain, S. Mohan, Sudhir K. Sopory, and R. E. Veilleux. In vitro haploid production in higher plants. Dordrecht: Kluwer Academic Publishers, 1996.

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Embryology, Interim Licensing Authority for Human in vitro Fertilisation and. Report of the Voluntary Licensing Authority for Human in vitro Fertilisation and Embryology. London: VLA., 1989.

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Gunning, Jennifer. Human IVF, embryo research, fetal tissue for research and treatment, and abortion: International information. London: H.M.S.O, 1990.

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Basu, P. K. Biological effects of extremely low frequency electric and magnetic fields on the ocular tissues: An in vitro study. Albany, N.Y: New York State Power Lines Project, 1986.

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Authority, Interim Licensing. The report of the Interim Licensing Authority for Human In Vitro Fertilisation and Embryology. London: The Authority, 1991.

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Voluntary Licensing Authority for Human In Vitro Fertilisation and Embryology. The report of the Voluntary Licensing Authority for Human In Vitro Fertilisation and Embryology. London: The authority., 1986.

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Voluntary Licensing Authority for Human In Vitro Fertilisation and Embryology. The Fourth report of the Voluntary Licensing Authorityfor Human in vitro fertilisation and embryology. London: Voluntary Licensing Authority, 1989.

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Voluntary Licensing Authority For Human In Vitro Fertilisation and Embryology. The second report of the Voluntary Licensing Authorityfor Human In Vitro Fertilisation and Embryology. London: Voluntary Licensing Authority, 1987.

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Book chapters on the topic "In vitro; Embryology"

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Ata, Baris, Jack Huang, and Ri-Cheng Chian. "In Vitro Maturation of Human Oocytes." In Clinical Embryology, 115–31. New York, NY: Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4614-8376-2_8.

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Scott, Lynette. "Pronuclear Scoring in Human In Vitro Fertilization." In Clinical Embryology, 267–75. New York, NY: Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4614-8376-2_15.

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Shoham, Zeev. "Journey of Human Gametes In Vitro: 1978–2010." In Clinical Embryology, 1–9. New York, NY: Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4614-8376-2_1.

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Bhojwani, Sant S., and T. Dennis Thomas. "In Vitro Gynogenesis." In Current Trends in the Embryology of Angiosperms, 489–507. Dordrecht: Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-017-1203-3_20.

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Kranz, E. "In Vitro Fertilization." In Current Trends in the Embryology of Angiosperms, 143–66. Dordrecht: Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-017-1203-3_7.

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Dvorak, Ann M. "In Vitro Studies of Human Mast Cells." In Advances in Anatomy Embryology and Cell Biology, 15–73. Berlin, Heidelberg: Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-74145-6_4.

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Marthy, Hans-Jürg, Luigia Santella, and Brian Dale. "In Vitro Preparation of the Early Squid Blastoderm." In Experimental Embryology in Aquatic Plants and Animals, 193–96. Boston, MA: Springer US, 1990. http://dx.doi.org/10.1007/978-1-4615-3830-1_10.

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Go, Kathryn J., Jay C. Patel, and Rick Dietz. "Troubleshooting in the Clinical Embryology Laboratory: The Art of Problem-Solving in ART." In Practical Manual of In Vitro Fertilization, 631–37. New York, NY: Springer New York, 2012. http://dx.doi.org/10.1007/978-1-4419-1780-5_71.

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Oorschot, Dorothy Eve, and David Gareth Jones. "In Vitro Experimental Approaches to Hypotheses Concerned with Regeneration in the Mammalian CNS." In Advances in Anatomy, Embryology and Cell Biology, 51–90. Berlin, Heidelberg: Springer Berlin Heidelberg, 1990. http://dx.doi.org/10.1007/978-3-642-75108-0_3.

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"In Vitro Fertilization (IVF)." In A Textbook of Clinical Embryology, 95–113. Cambridge University Press, 2021. http://dx.doi.org/10.1017/9781108881760.010.

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