Relevant bibliographies by topics / In vitro

Academic literature on the topic 'In vitro'

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Published: 4 June 2021
Last updated: 29 July 2024

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Journal articles on the topic "In vitro"

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Condori, Rosa, Carlos Quispe, Edith Ancco, Deysi Dipaz, and Edwin Mellisho. "SOBREVIVENCIA DE BLASTOCISTOS BOVINOS PRODUCIDOS IN VITRO VITRIFICADOS EN DISPOSITIVOS VITRI-TIP Y VITRI-TOP." SPERMOVA 9, no. 1 (August 31, 2019): 48–52. http://dx.doi.org/10.18548/aspe/0007.07.

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AKIMOVA, S. V., V. V. KIRKACH, A. K. RADZHABOV, and G. E. TER-PETROSYANTS. "MORPHOBIOLOGICAL FEATURES OF DIAPHRAGM FORMATION IN IN VITRO AND EX VITRO GRAPE PLANTS OF INTERSPECIFIC ORIGIN." Izvestiâ Timirâzevskoj selʹskohozâjstvennoj akademii, no. 6 (2021): 5–13. http://dx.doi.org/10.26897/0021-342x-2021-6-5-13.

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Clonal micropropagation of grape varieties of interspecific origin Moscow white (Vitis amurensis Rupr. × Vitis vinifera L.) showed that on the 40th day of the animation stage, a monopodial type of branching is typical for micro-plants, and there is no diaphragm in all nodes of micro-shoots. On the 120th day of growing in containers, the researchers revealed that the nodes of the plant shoots, from the 1st to the 6th, have a monopodial branching type. Parenchymal cells which form an incomplete diaphragm are present in the seventh node. A fully formed diaphragm appears from the eighth node, and the branching type switches to a sympodial-monopodial one.
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Teixeira da Silva, J. A. "CHRYSANTHEMUM: EX VITRO TO IN VITRO TO EX VITRO." Acta Horticulturae, no. 616 (November 2003): 443–47. http://dx.doi.org/10.17660/actahortic.2003.616.68.

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Mikhailova, Mikhailova N. D., Mishieva N. G. Mishieva, Kirillova A. O. Kirillova, Martazanova B. A. Martazanova B, and Dzhincharade L. G. Dzhincharade. "In vitro oocyte maturation." Akusherstvo i ginekologiia 11_2021 (November 26, 2021): 64–70. http://dx.doi.org/10.18565/aig.2021.11.64-70.

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Apóstolo, Nancy M., Ezequiel E. Larraburu, Miriam N. Gil, María A. Zapater, and Berta E. Llorente. "In vitro and ex vitro germination of three Handroanthus species (Bignoniaceae)." Bonplandia 25, no. 1 (January 1, 2016): 5. http://dx.doi.org/10.30972/bon.2511267.

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Handroanthus impetiginosus, H. lapacho and “H.ochraceuslapachos” se distribuyen en el NO Argentino y presentan inconvenientes de germinación y conservación en su ambiente natural. La germinación de semillas bajo condiciones controladas es una alternativa para asegurar la propagación de especies con este tipo de problemáticas. En el presente estudio integral, se analizó la germinación in vitroy ex vitro, las características de las semillas y la morfología de las plántulas de las tres especies de Handroanthusmencionadas. Para ello, se midió el largo y ancho de las semillas, el ancho de las alas de la cubierta seminal, el ancho y largo del cuerpo seminal y del embrión. El poder germinativo de las tres especies fue determinado durante 12 meses luego de la cosecha de las semillas. Fueron determinados los parámetros de las plántulas obtenidas in vitroy ex vitro. El tamaño de la semilla y embrión de H. impetiginosus.
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Zinatullina, A. E., and V. I. Nikonov. "LABORATORY EVALUATION OF REGENERATES OF WHEAT HYBRID COMBINATIONS IN VITRO AND EX VITRO CONDITIONS." ÈKOBIOTEH 4, no. 2 (2021): 81–88. http://dx.doi.org/10.31163/2618-964x-2021-4-2-81-88.

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Drought is the combination of climatic conditions that leads to a long-term shortage of water in the soil and air. This is one of the most common abiotic stress factors that leads to significant losses of crop yield and the emergence of a threat to food security. Researchers are actively developing ways to create drought-resistant zoned varieties of economically important agricultural crops and especially cereals as the main food resource. Such varieties should maintain a relatively high yield rate with a shortage of water in the soil and air. The aim of the work was the laboratory evaluation in vitro and ex vitro of wheat regenerants formed in the embryo culture in vitro under conditions selective for the indicator "drought resistance". Methods of embryo culture in vitro, laboratory evaluation of caryopsis viability, histological analysis, as well as statistical processing of the received results were used. Under the conditions of in vitro experiments on the selective medium simulating drought by introducing mannit at the concentration of 8% as an osmotic, regenerants of 5 hybrid wheat combinations that showed tolerance to stress were obtained. It is shown that the development of regenerants in vitro and ex vitro pass according to the same phenological phases and in the same duration as donor plants. Regenerants form caryopsises of sufficiently high quality, which is confirmed by laboratory observations of their viability and histological analysis of seedlings.
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Chakravarthy, U., D. McCormick, C. J. F. Maguire, and D. B. Archer. "An in-vitro study of irradiated vitreo-retinal membranes." Eye 1, no. 1 (January 1987): 126–35. http://dx.doi.org/10.1038/eye.1987.19.

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Harmanchuk, LV, OM Makarenko, NM Khranovs'ka, TV Nikolaienko, VV Nikulina, KhD Nepyĭvoda, LI Ostapchenko, SH Morozov, and MS Kositsyn. "Mitokorrektine stimulates angiogenesis in vitro." Fiziolohichnyĭ zhurnal 59, no. 2 (May 15, 2013): 52–58. http://dx.doi.org/10.15407/fz59.02.052.

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Babanina, Svetlana, Natalya Egorova, Olga Yakimova, and Mariya Kovalenko. "ADAPTATION TO EX VITRO CONDITIONS OF MICROPLANTS LAVANDULA ANGUSTIFOLIA MILL. AT LONG-TERM REPRODUCTION IN VITRO." Vestnik of Kazan State Agrarian University 18, no. 3 (October 2, 2023): 11–19. http://dx.doi.org/10.12737/2073-0462-2023-11-19.

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The purpose of the study is to identify the features of adaptation to ex vitro conditions of lavender plants after long-term clonal micropropagation. The experiments were carried out on microplants of narrow-leaved lavender (Lavandula angustifolia Mill.), cv. The number of plants in each subcultivation - n=10 pcs., 3-fold repetition. Microplants with well-developed shoots and roots were planted in a mixture of peat and perlite (1:1) and grown at illumination of 2–3 klx, photoperiod duration of 16 h, temperature of 24 ± 2°C, air humidity of 70%. The frequency of adaptation of microplants, depending on the number of subcultivations, varied slightly and amounted to 83...100%. On the 60th day of adaptation, the length of the shoot was significantly higher by 21...28% in microplants after 8 subcultivations (206.73 mm) compared with 14, 15 and 16 passages. There were no differences in the length of additional shoots depending on the amount of subculturing. According to the number of nodes on the main shoot, a tendency to their decrease with an increase in the number of passages was observed. A significant increase in the content of chlorophyll a with an increase in the number of subcultivations on the 14th day of adaptation was revealed, however, later these differences leveled out. On average, the in vitro viability index for passages was 1.45 and increased up to 30 days of adaptation to 1.75. The revealed features of changes in morphometric and physiological parameters indicate a good adaptive ability of the analyzed plants, while micropropagation in vitro during 16 subcultivations did not significantly reduce their adaptive potential. The optimal period of ex vitro adaptation is the period of 45...60 days, during which the plants formed well-developed shoots (3.20...6.00 g) and root system (0.619...1.143 g).
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Yurin, D. V., V. V. Nevzorova, A. A. Balbutskaya, and S. S. Belimova. "Antimicrobial activity of enrofloxacin in vitro." International bulletin of Veterinary Medicine 2 (2020): 99–103. http://dx.doi.org/10.17238/issn2072-2419.2020.2.99.

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Continuouse use of enrofloxacin contributes to emergence of enrofloxacin-resistant mi-crobial resistance, isolated and reported late-ly. In this study we deal with the spread of resistance of enrofloxacin among pathogenic organisms, infecting animals. The suscepti-bility to enrofloxacin was studied in standard disc diffusion assay. We studied 437 bacteri-al isolates in total. Salmonella dublin and Sal-monella typhimurium showed the highest suscepti-bility to enrofloxacin (100%); Salmonella enter-itidis and Salmonella choleraesuis proved a bit less susceptibility (95% and 94,7%). 5% of S. enter-itidis isolates and 5.3% of S. choleraesuis isolates had intermediate susceptibility. We did not register any resistance of isolates of Salmonella, Pasteurella and Morganella (Pasteurella multocida, Morganel-la morganii). 83.9% of Escherichiacoli strains proved susceptibility to enrofloxacin, the zone of retardation in 6.4% of the isolates was in corre-spondence with intermediate susceptibility, 9.7% of the isolates proved to be resistant. 90,9% of Pseudomonas aeruginosa isolates in our study was susceptible to enrofloxacin, 9.1% of them had intermediate susceptibility. The isolates of Strepto-coccus spp. and Staphylococcus pseudintermedius re-vealed high susceptibility to enrofloxacin, also as Listeria monocytogenes (causative agent of listeriosis)and Erysipe-lothrix rhusiopathiae (causative agent of swine erysipelas). 87.5% of the coagulase negative staphylococci proved susceptible to enrofloxacin; 6.25% of the isolates were resistant or had intermediate susceptibility. The shares of susceptible isolates of Staphylococcus hyicus, Staphylococ-cus aureus and Streptococcus uberis were respec-tively 65.1%, 75%, 75%. The shares of isolates with intermediate susceptibility of the same spp. were respectively 9.3%, 15%, 25%. The shares of resistant isolates of Staphylococci were respective-ly 25.6% and 10%. We found no strains of Str. uberis with resistance to enrofloxacin. As for Enterococci, 52.4% of the isolates were enrofloxacin-susceptible, 11,9% and 37,7% of them were re-spectively enrofloxacin-resistant or had intermedi-ate susceptibility. Presently most Gram-negative pathogenic bacteria have no resistance to enroflox-acin. Notwithstanding that enrofloxacin is signifi-cantly less effective against such pathogenic organ-isms as Staphylococci and Streptococci.
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Dissertations / Theses on the topic "In vitro"

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Nogueira, Jênifer Silva. "Germinação ex vitro e in vitro de gabirobeira." Universidade Federal de Goiás, 2012. http://repositorio.bc.ufg.br/tede/handle/tede/7497.

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Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq
The genus Campomanesia spp. has great economic potential unexploed. How its seeds not tolerate desiccation its propagations has been made by seeds extraction, when they are in germinate at appropriate physiological conditions. Several problems such as dormancy, recalcitrance, seasonality, difficulty of propagation has difficult he rational exploration of various species. Environmental conditions appropriate for the germination process may be provided in laboratories by in vitro multiplication. In this work techniques were applied to study some factors that affect ex vitro and in vitro germination of gabirobeira seeds and anther and ovaries culture through micropropagation. The results indicated that ex vitro germination the test substrates (Sand, Bioplant®, Tri-mix®, Bioplant®+Sand (1:1 v/v), Tri-mix®+Sand) for all substrates were superior to Tri-mix® substrate for germination percentage and for emergency speed of index. For the in vitro germination was found that all the culture medium tested in the absence of sucrose had a satisfactory germination rate. Evaluating the effects of accelerated aging and GA3 in the in vitro germination conclude that premature aging interfered the gabirobeira seeds ability of germination and GA3 is unnecessary for the germination stage. In the anthers and ovaries of culture, no callus formation in any of the treatments. However, the PVP was effective as an antioxidant. Keywords: sucrose,
A gabirobeira pertence ao gênero Campomanesia spp. possui grande potencial econômico não explorado. Como suas sementes não toleram a dessecação, sua propagação tem sido realizada logo após a extração, quando estas estão em condições fisiológicas apropriadas para germinar. Vários problemas como dormência, recalcitrância, sazonalidade e dificuldades de propagação têm inviabilizado a exploração econômica racional de várias espécies. Condições ambientais apropriadas para o processo de germinação podem ser fornecidas em laboratórios por meio da multiplicação in vitro. Neste trabalho, foram aplicadas técnicas para estudar alguns fatores que interferem na germinação ex vitro e in vitro de sementes de gabiroba e na micropropagação através da cultura de anteras e ovários. Os resultados indicaram que ao testar os substratos (Areia, Bioplant®, Tri-mix®, Bioplant®+Areia (1:1 v/v), Trimix ®+Areia)todos os substratos foram superiores ao substrato Tri-mix® para a porcentagem de germinação e vigor. Para a germinação in vitro verificou-se que todos os meios de cultura testados (MS, MS meia força, WPM e WPM meia força) na ausência de sacarose tiveram uma taxa de germinação satisfatória. Avaliando os efeitos do envelhecimento acelerado e de GA3 na germinação in vitro, o envelhecimento acelerado interferiu na capacidade germinativa das sementes de gabiroba e verificou-se que a aplicação de GA3 exógeno é desnecessária para a etapa de germinação. Na cultura de anteras e ovários, não houve calogênese em nenhum dos tratamentos testados. No entanto, o PVP foi eficiente como antioxidante.
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Christie, Louisa A. "Homocysteine alters hippocampal signalling in vitro and ex vitro." Thesis, University of Aberdeen, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.440060.

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The focus of this thesis was to investigate the non-essential, sulphur-containing amino acid homocysteine (HCY); elevations in plasma HCY have been associated with several pathological states in the elderly; age-associated reductions in vitamins, essential for the metabolism of HCY, are common and this situation is exacerbated during Alzheimer’s disease (AD).  Primary culture preparations of hippocampal tissue were utilised to investigate intracellular signalling and Ca2+ homeostasis, which were monitored using the Ca2+ fluorescent dye Fluo-4.  In the present study hippocampal slices were prepared from adult rats.  In Fluo 4 Ca2+ imaging studies, HCY (10, 100 µM and 1 mM) added acutely, caused rises in intracellular Ca2+ and decreased NMDA-induced Ca2+ responses.  Pharmacological investigations confirmed that the primary Ca2+ influx following addition of HCY (1 mM) was not mediated via NMDA, group I mGluRs, glycine or GABA receptors, voltage-gated Ca2+ channels (VGCCs) or intracellular stores (ER).  Results with specific antagonists indicate that HCY may have a partial interaction with NMDA receptors and intracellular stores; additionally, experiments revealed that HCY may antagonise the glycine co-agonist binding site on the NMDA receptor and may depend of HCY dose or competition with other glycine site modulators.  Acute application of HCY in slices (10 µM - 1 mM) in vitro caused a dose-response effect with lower concentrations impairing and higher doses enhancing LTP.  With different systemic routes and longer durations (with two concentrations: 20 and 200 mg/kg) basic transmission and LTP were altered in a bi-directional as well as concentration- and time-dependent manner, suggestive of diverse and complex targets for HCY.  Overall, the observed HCY-induced functional alterations amid lack of severe toxicity in vitro and ex vivo in healthy cells suggest a possible contribution in aged or diseased tissues, making this an extremely important consideration for future research as well as for dietary considerations in the elderly.
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Gomes, Daniela Faria. "Compósitos de PCL e vidro bioativo : estudo do comportamento in vitro." Master's thesis, Universidade de Aveiro, 2013. http://hdl.handle.net/10773/12323.

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Mestrado em Materiais e Dispositivos Biomédicos
Na regeneração do tecido ósseo torna-se cada vez mais frequente o recurso a materiais capazes de interagir com o organismo, através de um conjunto de respostas específicas que conduzem à formação de novo tecido ósseo. Neste contexto, os compósitos de matriz polimérica com enchimento bioativo têm sido objeto de investigação recente pois combinam as vantagens destes dois tipos de materiais, para as aplicações pretendidas. O presente trabalho teve como objetivo desenvolver compósitos de vidro bioativo e policaprolactona (PCL) e estudo do seu comportamento em meio acelular (in vitro). Quando utilizados separadamente em aplicações biomédicas, estes materiais têm revelado características promissoras. No entanto, as propriedades de compósitos de matriz de PCL reforçado com vidro bioativo ainda não estão totalmente esclarecidas. O vidro em estudo pertence ao sistema 3CaO-P2O5-SiO2-MgO, identificado em estudos anteriores, como possuindo características bioativas. O PCL foi escolhido por ser um polímero sintético biocompatível, biodegradável e aprovado pela Food and Drug Administration (FDA). Os compósitos foram preparados por duas técnicas distintas: extrusão e evaporação do solvente. Estudaram-se algumas variáveis de processamento, nomeadamente: a fração de partículas vítreas, o tamanho de partículas do enchimento, fração de partículas vítreas e respetiva distribuição granulométrica. O comportamento destes compósitos em meio fisiológico simulado indica um elevado potencial bioativo, manifestado através da formação de uma camada de fosfato de cálcio na sua superfície, o que torna estes compósitos promissores para aplicações em regeneração do tecido ósseo.
In bone regeneration it becomes more and more attractive the use of materials capable of interacting with the living tissues through adequate responses that lead to the formation of new bone. In this context, composites of polymeric matrix with bioactive filling composites have recently been investigated because they combine the advantages of these two types of materials for the envisaged application. The main goal of this work was to produce composites of polycaprolactone (PCL) and a bioactive glass as filler and to evaluate their in vitro behavior in an acelular medium When used separately in biomedical applications, these materials have revealed quite promising characteristics. However, the properties of composites based on polymeric matrix reinforced with glass are not yet entirely clarified. The bioactive glass used in this project belongs to the 3CaO-P2O5-SiO2-MgO, and system showed, in previous works, to exhibit a bioactive behavior in vitro. PCL was chosen because it is a synthetic biocompatible, biodegradable and Food and Drug Administration (FDA) approved polymer. The composites were prepared by two techniques: extrusion and solvent casting. Some variables of processing were studied, namelly: the filler containt, the particle size and particle size distribution. The behavior of these composites in simulated physiologic environment indicates a high bioactive potential confirmed by the formation of a calcium phosphate layer, making these composites very promising for bone regeneration applications.
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Marangoni, Alessandra. "Propagação ex vitro e in vitro de Heliconia angusta VELL." Florianópolis, SC, 2001. http://repositorio.ufsc.br/xmlui/handle/123456789/80244.

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Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias. Curso de Pós-Graduação em Recursos Genéticos Vegetais
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Considerando as limitações das técnicas convencionais de propagação vegetativa para esta espécie, o estabelecimento de metodologia para a propagação vegetativa é de fundamental importância para ampliar as áreas de cultivo e atender a demanda do mercado consumidor. Assim, no presente trabalho, objetivou-se realizar estudos visando otimizar as formas de propagação vegetativa desta espécie. Dois sistemas foram empregados para propagação vegetativa de Heliconia angusta. No primeiro, foi avaliado a propagação vegetativa através da divisão de rizomas onde foi avaliado as respostas destas estruturas frente a utilização de benzilaminopurina (BAP). No segundo sistema de produção, procurou-se estabelecer parâmetros para cultura in vitro desta espécie, em especial o controle da oxidação e da contaminação dos explantes.
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Curti, Aline Ritter. "RIZOGÊNESE IN VITRO E EX VITRO EM Peltophorum dubium (SPRENGEL) TAUBERT." Universidade Federal de Santa Maria, 2014. http://repositorio.ufsm.br/handle/1/3767.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Peltophorum dubium is native forest species of Brazil endowed with ecological importance for the recovery of degraded areas and economical for timber purposes. The seeds of this species have low and irregular germination if they are not subjected to treatments to overcome dormancy. However, there are few studies that allow for the vegetative propagation of the species. As a result, the present study aimed to evaluate the formation of roots in shoots of P. dubium applying the techniques of micropropagation and minicutting and acclimatization of plants produced by these techniques. For rooting in vitro micropropagated shoots were subjected to treatments where different culture media were tested, with addition of 30 g L-1 vermiculite, combined with the nutrient media MS, MS/2, WPM, WPM/2 and changes in concentrations of auxin IBA (0, 5, 10 or 20 μM) and sucrose (0, 15 or 30 g L -1) in the presence or absence of agar (0 or 7g L-1) arranged in different tests. The results indicated that the best combination for root formation in vitro shoots of Peltophorum dubium is the use of nutritional WPM/2 without addition of sucrose and auxin, with 7 g L- 1 agar. This condition was still allowing the best performance of the plants during acclimatization in vitro. For rooting of shoots via minicutting, cuttings 3-7 cm apical portion of rooted shoots of seminal origin were used. Root formation after the cuttings are subjected to immersion in solution with 0, 1.000, 2.000 or 4.000 mg L- 1 IBA, from different collections and during 30, 60 or 90 days in a greenhouse with controlled conditions was evaluated for root induction. Rooting in cuttings occurred even without the use of IBA, after successive collections. However, it was increased with the use of up to 2.000 mg L-1 auxin the period of 60 days being sufficient for the formation of roots takes place. The seedlings showed good performance during acclimatization, indicating that the technique is feasible for minicutting plants production Peltophorum dubium.
Peltophorum dubium é uma espécie florestal nativa do Brasil dotada de importância ecológica para fins de recuperação de áreas degradadas e econômica, para fins madeiráveis. As sementes desta espécie apresentam germinação baixa e irregular se não forem submetidas a tratamentos para superação de dormência. Em virtude da carência de estudos que viabilizem a propagação vegetativa da espécie e considerando a natural recalcitrância em relação à rizogênese nesse processo, o presente trabalho teve como objetivo avaliar a rizogênese in vitro e ex vitro em brotações de P. dubium aplicando-se as técnicas de micropropagação e de miniestaquia, bem como a aclimatização das mudas produzidas por meio destas técnicas. Para a rizogênese in vitro, brotações micropropagadas foram submetidas a tratamentos em que foram testados diferentes meios de cultivo, com acréscimo de 30 g L-1 de vermiculita, combinados aos meios nutritivos MS, MS/2, WPM, WPM/2 e alterações nas concentrações da auxina ácido indolbutírico - AIB (0, 5, 10 ou 20 μM) e sacarose (0, 15 ou 30 g L-1), na presença ou ausência de ágar (0 ou 7 g L-1), arranjados em diferentes ensaios. Os resultados indicaram que a melhor combinação para a rizogênese in vitro em brotações de Peltophorum dubium é a utilização do meio nutritivo WPM/2, na ausência de sacarose e da auxina, porém com 7 g L-1 de ágar. Essa condição foi, ainda, a que permitiu o melhor desempenho das mudas durante a aclimatização in vitro. Para a rizogênese em brotações via miniestaquia, foram utilizadas miniestacas, de 3 a 7 cm, isoladas da porção apical de brotações de minicepas de origem seminal. Foi avaliada a formação de raízes após as miniestacas serem submetidas à imersão em solução contendo 0, 1.000, 2.000 ou 4.000 mg L-1 de AIB, oriundas de diferentes coletas e permanecendo por 30, 60 ou 90 dias em casa de vegetação com condições controladas adequadas à indução de raízes. A rizogênese nessas miniestacas ocorreu mesmo sem a utilização de AIB, ao longo das sucessivas coletas. No entanto, foi incrementada com a utilização de até 2.000 mg L-1 da auxina, sendo suficiente o período de 60 dias para que ocorra a formação de raízes. As mudas apresentaram bom desempenho durante a aclimatização, indicando que a técnica de miniestaquia é viável para a produção de mudas de Peltophorum dubium.
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Betton, Jean-Michel. "Étude du repliement in-vitro de la phosphoglycérate kinase in-vitro." Paris 11, 1987. http://www.theses.fr/1987PA112277.

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Cette thèse présente l’étude de la transition de dénaturation­ renaturation de la phosphoglycérate kinase (PGK) induite par le chlorure de guanidine. Dans la première partie, la transition conformationnelle a été étudiée à l’équilibre à l’aide de trois signaux: l’activité enzymatique, la fluorescence des tryptophanes et l’ellipcité. A partir de ces données, il a éte clairement constaté que le processus dévie significativement d’un modèle à deux états et qu’il existe des intermédiaires: les courbes de transition obtenues par les différents signaux ne coïncident pas et celle mesurée par dichroïsme circulaire est nettement asymétrique. Dans la seconde partie, les cinétiques de dénaturation et de renaturation ont été suivies par les mêmes signaux. Nous avons pu détecter plusieurs phases cinétiques. Les cinétiques expérimentales ont été comparées aux propriétés cinétiques de plusieurs schémas simulés. Un schéma minimum, tenant compte des diverses observations expérimentale été proposé. Dans la troisième partie, la réversibilité du processus de dénaturation a été étudiée en mesurant la réapparition de l’activité enzymatique. Certaines conditions expérimentales (0,8 M de chlorure de guanidine) permettent de piéger un intermédiaire structuré mal dépourvu de propriétés fonctionnelles. Cet intermédiaire correspond à une partie structurée du domaine C-terminal de la PGK. Ceci a été confirmé dans la dernière partie de la thèse où est présentée l’obtention du domaine C-terminal isolé par protéolyse ménagée. En conclusion, nous proposons un schéma pour la formation de la structure fonctionnelle de la phosphoglycérate kinase.
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7

Kruger, Francois Jacobus Liebenberg. "In Vitro and In Vitro production of artemisinin by artemisia species." Diss., University of Pretoria, 2004. http://hdl.handle.net/2263/40342.

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Artemisinin is produced in the leaves of Artemisia annua and is currently one of the most valuable antimalarial treatments. A. annua is of Asian origin but many other family members have been identified worldwide. A. annua however, is the only one that produces artemisinin. Synthetic production of artemisinin is not yet feasible, not to mention very expensive and the product yields are relatively low. The aims of this study were threefold: 1) To regenerate callus, cell cultures and plants from genetically modified root cultures of A. afra into which an artemisinin biosynthetic gene was inserted from A. annua 2) To investigate the probability that fungal endophytes are responsible for the production of artemisinin and 3) To establish two fields of high yielding varieties of A. annua plants and evaluate whether artemisinin production of these two locations will remain high. Callus and cell cultures of the genetically modified A. afra root cultures were established, but no shoots have been produced as of yet and this is an on-going investigation. Fungal endophytes were sampled and none of the endophytes produced artemisinin. Five different lines of A. annua were cultivated, successfully grown and harvested. Measurements were taken at different stages of processing, these were compared and analysed using various methods such as height and mass comparisons. Comparisons revealed that the production of artemisinin is correlated to local sets of conditions rather than the variety of individual lines. The genetic potential to produce high quantities of artemisinin appears to have been lost, instead of being maintained. We confirmed that secondary compound production and specifically, artemisinin, is enhanced by certain stress factors on the plants.
Dissertation (MSc)--University of Pretoria, 2013.
gm2014
Plant Science
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8

Odinot, Françoise. "Psychosomatique et fécondation in vitro." Bordeaux 2, 1995. http://www.theses.fr/1995BOR2M026.

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McKenna, Erin N. "Embryonic policies the stunted development of in vitro fertilization in the United States, 1975-1992 /." Connect to this title online, 2006. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=bgsu1143490658.

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Nitard-Plan, Sylvie. "Bilan de l'activité fécondation in vitro du centre d'aide médicale à la procréation du Vaucluse de 1987 à 1994." Montpellier 1, 1996. http://www.theses.fr/1996MON11032.

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Books on the topic "In vitro"

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Szymański, Łukasz. In vitro. Kraków: Petrus, 2009.

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Tiffany-Castiglioni, Evelyn, and Mannfred A. Hollinger, eds. In Vitro Neurotoxicology. Totowa, NJ: Humana Press, 2004. http://dx.doi.org/10.1385/1592596517.

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Reeves, Andrew, ed. In Vitro Mutagenesis. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6472-7.

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Ginsburg, Elizabeth S., and Catherine Racowsky, eds. In Vitro Fertilization. New York, NY: Springer New York, 2012. http://dx.doi.org/10.1007/978-1-4419-9848-4.

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Costa, Lucio G., Gennaro Giordano, and Marina Guizzetti, eds. In Vitro Neurotoxicology. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-170-3.

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Nagy, Zsolt Peter, Alex C. Varghese, and Ashok Agarwal, eds. In Vitro Fertilization. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-319-43011-9.

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Rațiu, Daniela. In vitro: Roman. București: Cartea Românească, 2006.

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A, Emanuel Ivor, ed. In vitro testing. New York: Thieme Medical Publishers, 1994.

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service), ScienceDirect (Online, ed. Microtubules, in vitro. Amsterdam: Elsevier/Academic Press, 2010.

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1948-, Gad Shayne C., ed. In vitro toxicology. New York: Raven Press, 1994.

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Book chapters on the topic "In vitro"

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Heppner, John B., D. G. Boucias, J. C. Pendland, Andrei Sourakov, Timothy Ebert, Roger Downer, Kun Yan Zhu, et al. "In Vitro." In Encyclopedia of Entomology, 2044. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6359-6_1581.

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Andrae, U. "Genotoxizitätstestsin vitro." In Toxikologie, 321–41. Weinheim, FRG: Wiley-VCH Verlag GmbH & Co. KGaA, 2005. http://dx.doi.org/10.1002/3527604820.ch20.

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Arndt, T. "In vitro." In Lexikon der Medizinischen Laboratoriumsdiagnostik, 1. Berlin, Heidelberg: Springer Berlin Heidelberg, 2017. http://dx.doi.org/10.1007/978-3-662-49054-9_1561-1.

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Arndt, T. "In vitro." In Springer Reference Medizin, 1276. Berlin, Heidelberg: Springer Berlin Heidelberg, 2019. http://dx.doi.org/10.1007/978-3-662-48986-4_1561.

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Bährle-Rapp, Marina. "in vitro." In Springer Lexikon Kosmetik und Körperpflege, 278. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-71095-0_5176.

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Tiffany-Castiglioni, Evelyn. "In Vitro Neurotoxicology." In In Vitro Neurotoxicology, 1–28. Totowa, NJ: Humana Press, 2004. http://dx.doi.org/10.1385/1-59259-651-7:1.

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Or, Yuval, Shir Dar, and Zeev Shoham. "Journey of Human Gametes In Vitro: 1978 to 2018." In In Vitro Fertilization, 1–6. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-319-43011-9_1.

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Meintjes, Marius. "CO2 and Low-O2 Incubators." In In Vitro Fertilization, 85–94. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-319-43011-9_10.

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Johansson, Lars. "What to Consider When Selecting a LAF, Class II Cabinet, or Isolette for Your ART Program?" In In Vitro Fertilization, 95–103. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-319-43011-9_11.

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Swain, Jason E. "Culture Media in IVF: Decisions for the Laboratory." In In Vitro Fertilization, 105–19. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-319-43011-9_12.

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Conference papers on the topic "In vitro"

1

Cambre, Julia, Ying Liu, Rebecca E. Taylor, and Chinmay Kulkarni. "Vitro." In DIS '19: Designing Interactive Systems Conference 2019. New York, NY, USA: ACM, 2019. http://dx.doi.org/10.1145/3322276.3322298.

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Batukaev, Abdulmalik. "IN VITRO MICROCLONAL PROPAGATION OF STRAWBERRIES AND EX VITRO ADAPTATION." In 19th SGEM International Multidisciplinary Scientific GeoConference EXPO Proceedings. STEF92 Technology, 2019. http://dx.doi.org/10.5593/sgem2019/6.1/s25.095.

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Atigh, Marzieh K., and Saami K. Yazdani. "In Vitro Stent Endothelialization." In 2016 32nd Southern Biomedical Engineering Conference (SBEC). IEEE, 2016. http://dx.doi.org/10.1109/sbec.2016.24.

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Alatortseva, T. A. "Petchoa in vitro culture." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.015.

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Batukayev, A. A., D. О. Palaeva, М. S. Batukaev, and E. А. Sobralieva. "In Vitro Reproduction and Ex Vitro Adaptation of Complex Resistant Grape Varieties." In International scientific and practical conference "AgroSMART - Smart solutions for agriculture" (AgroSMART 2018). Paris, France: Atlantis Press, 2018. http://dx.doi.org/10.2991/agrosmart-18.2018.168.

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Cagnin Vicente, Michele, Rogério Gomes Pêgo, and Margarida Goréte Ferreira do Carmo. "GERMINAÇÃO EX VITRO E IN VITRO DE SEMENTES DE Pleroma heteromallum (MELASTOMATACEAE)." In IV Workshop do PPG-Fitotecnia "Desafios e Oportunidades da Produção Agrícola no Cenário Atual". ,: Even3, 2022. http://dx.doi.org/10.29327/fitotecnia2022.498232.

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Brittain, S. B., J. L. Hajjar, and S. S. Nidadavolu. "In-vitro hemostasis test platform." In 2011 37th Annual Northeast Bioengineering Conference (NEBEC). IEEE, 2011. http://dx.doi.org/10.1109/nebc.2011.5778553.

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Nakamura, O., D. J. Fink, D. P. Lennon, V. L. Laraia, A. H. Heuer, and A. I. Caplan. "MATRIX - DIRECTED IN VITRO OSTEOGENESIS." In Proceedings of the 12th International Symposium on Ceramics in Medicine. WORLD SCIENTIFIC, 1999. http://dx.doi.org/10.1142/9789814291064_0060.

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Nel, Jan-Gert, Chrisna Durnadt, Timothy Mitchell, Charles Feldman, Ronald Anderson, and Gregory Tintinger. "Pneumolysin mediates platelet activationin vitro." In ERS International Congress 2016 abstracts. European Respiratory Society, 2016. http://dx.doi.org/10.1183/13993003.congress-2016.pa2593.

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Batukaev, M. S., D. O. Palaeva, and A. A. Batukaev. "MICROPROPAGATION OF GRAPES IN VITRO." In The All-Russian Scientific Conference with International Participation and Schools of Young Scientists "Mechanisms of resistance of plants and microorganisms to unfavorable environmental". SIPPB SB RAS, 2018. http://dx.doi.org/10.31255/978-5-94797-319-8-1172-1175.

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Reports on the topic "In vitro"

1

Harbell, John W. 2001 Congress on In Vitro Biology. Fort Belvoir, VA: Defense Technical Information Center, July 2002. http://dx.doi.org/10.21236/ada407495.

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Harbell, John W. 2002 Congress on In Vitro Biology. Fort Belvoir, VA: Defense Technical Information Center, June 2002. http://dx.doi.org/10.21236/ada408075.

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Harbell, John W. 2003 Congress on In Vitro Biology. Fort Belvoir, VA: Defense Technical Information Center, May 2004. http://dx.doi.org/10.21236/ada426877.

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Frazier, John M. In Vitro Approach To Predictive Toxicokinetics. Fort Belvoir, VA: Defense Technical Information Center, November 1995. http://dx.doi.org/10.21236/ada305752.

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Liu, Hong, and Myles Brown. Estrogen Receptor-Mediated Transcription In Vitro. Fort Belvoir, VA: Defense Technical Information Center, December 1997. http://dx.doi.org/10.21236/ada340593.

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Liu, Hong, and Virgil C. Jordan. Estrogen Receptor-Mediated Transcription In Vitro. Fort Belvoir, VA: Defense Technical Information Center, December 1999. http://dx.doi.org/10.21236/ada381241.

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Liu, Hong. Estrogen Receptor-Mediated Transcription in Vitro. Fort Belvoir, VA: Defense Technical Information Center, December 1998. http://dx.doi.org/10.21236/ada384349.

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Giordano, Federica. SOP for In Vitro Spheroid Formation. ResearchHub Technologies, Inc., April 2024. http://dx.doi.org/10.55277/researchhub.i4casfoc.

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Out!, Scientists. In-vitro embryos: can photonics help? ResearchHub Technologies, Inc., May 2024. http://dx.doi.org/10.55277/researchhub.h6rkjaef.

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Harbell, John. 2000 World Congress on In Vitro Biology. Fort Belvoir, VA: Defense Technical Information Center, September 2001. http://dx.doi.org/10.21236/ada395956.

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