Relevant bibliographies by topics / In-Situ microscopie de fluorescence

Academic literature on the topic 'In-Situ microscopie de fluorescence'

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Published: 25 May 2024

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Journal articles on the topic "In-Situ microscopie de fluorescence":

1

Pernthaler, Jakob, Annelie Pernthaler, and Rudolf Amann. "Automated Enumeration of Groups of Marine Picoplankton after Fluorescence In Situ Hybridization." Applied and Environmental Microbiology 69, no. 5 (May 2003): 2631–37. http://dx.doi.org/10.1128/aem.69.5.2631-2637.2003.

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ABSTRACT We describe here an automated system for the counting of multiple samples of double-stained microbial cells on sections of membrane filters. The application integrates an epifluorescence microscope equipped with motorized z-axis drive, shutters, and filter wheels with a scanning stage, a digital camera, and image analysis software. The relative abundances of specific microbial taxa are quantified in samples of marine picoplankton, as detected by fluorescence in situ hybridization (FISH) and catalyzed reporter deposition. Pairs of microscopic images are automatically acquired from numerous positions at two wavelengths, and microbial cells with both general DNA and FISH staining are counted after object edge detection and signal-to-background ratio thresholding. Microscopic fields that are inappropriate for cell counting are automatically excluded prior to measurements. Two nested walk paths guide the device across a series of triangular preparations until a user-defined number of total cells has been analyzed per sample. A backup autofocusing routine at incident light allows automated refocusing between individual samples and can reestablish the focal plane after fatal focusing errors at epifluorescence illumination. The system was calibrated to produce relative abundances of FISH-stained cells in North Sea samples that were comparable to results obtained by manual evaluation. Up to 28 preparations could be analyzed within 4 h without operator interference. The device was subsequently applied for the counting of different microbial populations in incubation series of North Sea waters. Automated digital microscopy greatly facilitates the processing of numerous FISH-stained samples and might thus open new perspectives for bacterioplankton population ecology.
2

Leger, I., M. Robert-Nicoud, and G. Brugal. "Combination of DNA in situ hybridization and immunocytochemical detection of nucleolar proteins: a contribution to the functional mapping of the human genome by fluorescence microscopy." Journal of Histochemistry & Cytochemistry 42, no. 2 (February 1994): 149–54. http://dx.doi.org/10.1177/42.2.8288860.

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The recent application of DNA cloning and non-radioactive in situ hybridization techniques has strengthened the hypothesis of an ordered chromatin structure in interphase nuclei. The arrangement of specific chromosomal regions is not random and is strongly suspected to vary with functional activity. The combination of in situ hybridization and immunocytochemistry, allowing simultaneous detection of nucleic acid sequences and specific antigens in the same nucleus, has already made significant contributions to the study of gene expression, to simultaneous karyotyping and phenotyping of tumor cells, and to in situ analysis of viral infections. This report emphasizes the considerable interest of such combined techniques for functional in situ mapping of the genome at the individual cell level. We propose a method that combines fluorescence immunocytochemical detection of nucleolar proteins and fluorescence in situ hybridization of centromeric and telomeric probes specific for chromosome 1 in two cultured human cell lines. The preparative constraints for a broad application of this procedure are defined so that the cell preparations can be further analyzed by fluorescence microscopic imaging techniques and confocal laser scan microscopy. The two selected sequences of the human chromosome 1 can be localized in the nucleus with respect to nucleolar proteins in a one-step fluorescence microscopic observation.
3

Lu, Fang, Tingting Zhou, Yan Liu, Liying Song, Bin Zhang, and Yuyan Li. "Application of Fluorescence In Situ Hybridization Assisted by Fluorescence Microscope in Detection of Her2 Gene in Breast Cancer Patients." Contrast Media & Molecular Imaging 2022 (August 11, 2022): 1–6. http://dx.doi.org/10.1155/2022/3087681.

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In order to study the important factors for evaluating the prognosis of breast cancer patients, a fluorescence microscopy-assisted fluorescence in situ hybridization technique was proposed. Compared with other detection techniques, fluorescence in situ hybridization (FISH) technology assisted by a fluorescence microscope has gradually gained favor in related fields due to its advantages of high detection specificity, high sensitivity, and strong experimental period. Combined with the basic overview of fluorescence microscopy and FISH technology, the advantages and application points of FISH technology assisted by fluorescence microscopy in the detection of the Her2 gene in breast cancer patients were studied and discussed. The results show that IHC can be used as the primary screening for HER2 gene status detection; IHC (2+) and IHC (3+) have false positives, which are related to chromosome 17 polysomy, so FISH should be done to confirm the diagnosis.
4

Buchanan, Bailey C., and Jeong-Yeol Yoon. "Microscopic Imaging Methods for Organ-on-a-Chip Platforms." Micromachines 13, no. 2 (February 19, 2022): 328. http://dx.doi.org/10.3390/mi13020328.

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Microscopic imaging is essential and the most popular method for in situ monitoring and evaluating the outcome of various organ-on-a-chip (OOC) platforms, including the number and morphology of mammalian cells, gene expression, protein secretions, etc. This review presents an overview of how various imaging methods can be used to image organ-on-a-chip platforms, including transillumination imaging (including brightfield, phase-contrast, and holographic optofluidic imaging), fluorescence imaging (including confocal fluorescence and light-sheet fluorescence imaging), and smartphone-based imaging (including microscope attachment-based, quantitative phase, and lens-free imaging). While various microscopic imaging methods have been demonstrated for conventional microfluidic devices, a relatively small number of microscopic imaging methods have been demonstrated for OOC platforms. Some methods have rarely been used to image OOCs. Specific requirements for imaging OOCs will be discussed in comparison to the conventional microfluidic devices and future directions will be introduced in this review.
5

Tatarov, Boyan, Detlef Müller, Matthias Tesche, and Sung-Kyun Shin. "Lidar Innovations for Technologies and Environmental Sciences (LITES) – An Remote Sensing Infrastructure Facility: Setup and Measurements Examples." EPJ Web of Conferences 237 (2020): 07017. http://dx.doi.org/10.1051/epjconf/202023707017.

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At the University of Hertfordshire, we have been developing a new remote sensing facility (LITES) to explore the feasibility of using Raman and/or fluorescence backscattering for chemical aerosol profiling. This paper provides an overview of the instruments of the facility and measurement examples. LITES includes a ultra-high-energy Nd:YAG/OPO setup, spectroscopic equipment with high spectral resolution, several imaging and single detectors that allow for time-resolved (lidar) signal detection, a Raman/fluorescence microscope, and a suite of gas and aerosol chambers. We present examples of elastic, rotational and vibrational spectroscopic lidar signals, as well as in-situ microscopic spectrums of dust and bio-aerosol compounds.
6

Leitch, A. R., W. Mosgoller, T. Schwarzacher, M. D. Bennett, and J. S. Heslop-Harrison. "Genomic in situ hybridization to sectioned nuclei shows chromosome domains in grass hybrids." Journal of Cell Science 95, no. 3 (March 1, 1990): 335–41. http://dx.doi.org/10.1242/jcs.95.3.335.

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In situ hybridization using biotinylated total genomic DNA and avidin detection systems was adapted for examination of thin-sectioned plant material in the light and electron microscopes. Root tip material was preserved prior to sectioning, so that the in vivo disposition of the chromatin was maintained. Use of total genomic DNA from Secale africanum as a probe enabled the chromatin from the two parental genomes in the grass hybrid Hordeum chilense × S. africanum to be distinguished. The biotinylated probe preferentially labelled the chromosomes of S. africanum origin. DNA-DNA hybrids were visualized at the light-microscope level by Texas Red fluorescence and at the electron-microscope level by the enzymic precipitation of DAB (diaminobenzidine) or by colloidal gold particles. The use of thin sections allowed the location of probe hybridization to be established unequivocally in both metaphase and interphase nuclei. Analysis of interphase nuclei showed that chromatin originating from the two parental genomes did not intermix but occupied distinct domains.
7

Deerinck, Thomas J., Maryann E. Martone, Varda Lev-Ram, David P. L. Green, Roger Y. Tsien, David L. Spector, Sui Huang, and Mark H. Ellisman. "3-Dimensional immunolabeling and in situ hybridization detection using fluorescence photooxidation and intermediate-voltage Electron Microscopy." Proceedings, annual meeting, Electron Microscopy Society of America 52 (1994): 164–65. http://dx.doi.org/10.1017/s0424820100168554.

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The confocal laser scanning microscope has become a powerful tool in the study of the 3-dimensional distribution of proteins and specific nucleic acid sequences in cells and tissues. This is also proving to be true for a new generation of high contrast intermediate voltage electron microscopes (IVEM). Until recently, the number of labeling techniques that could be employed to allow examination of the same sample with both confocal and IVEM was rather limited. One method that can be used to take full advantage of these two technologies is fluorescence photooxidation. Specimens are labeled by a fluorescent dye and viewed with confocal microscopy followed by fluorescence photooxidation of diaminobenzidine (DAB). In this technique, a fluorescent dye is used to photooxidize DAB into an osmiophilic reaction product that can be subsequently visualized with the electron microscope. The precise reaction mechanism by which the photooxidation occurs is not known but evidence suggests that the radiationless transfer of energy from the excited-state dye molecule undergoing the phenomenon of intersystem crossing leads to the formation of reactive oxygen species such as singlet oxygen. It is this reactive oxygen that is likely crucial in the photooxidation of DAB.
8

Jovin, Thomas M., Michel Robert-Nicoud, Donna J. Arndt-Jovin, and Thorsten Schormann. "3-D imaging of cells using a confocal laser scanning microscope and digital image processing." Proceedings, annual meeting, Electron Microscopy Society of America 46 (1988): 96–97. http://dx.doi.org/10.1017/s0424820100102560.

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Light microscopic techniques for visualizing biomolecules and biochemical processes in situ have become indispensable in studies concerning the structural organization of supramolecular assemblies in cells and of processes during the cell cycle, transformation, differentiation, and development. Confocal laser scanning microscopy offers a number of advantages for the in situ localization and quantitation of fluorescence labeled targets and probes: (i) rejection of interfering signals emanating from out-of-focus and adjacent structures, allowing the “optical sectioning” of the specimen and 3-D reconstruction without time consuming deconvolution; (ii) increased spatial resolution; (iii) electronic control of contrast and magnification; (iv) simultanous imaging of the specimen by optical phenomena based on incident, scattered, emitted, and transmitted light; and (v) simultanous use of different fluorescent probes and types of detectors.We currently use a confocal laser scanning microscope CLSM (Zeiss, Oberkochen) equipped with 3-laser excitation (u.v - visible) and confocal optics in the fluorescence mode, as well as a computer-controlled X-Y-Z scanning stage with 0.1 μ resolution.
9

Shah, Jyotsna S., Olivia Mark, Eddie Caoili, Akhila Poruri, Richard I. Horowitz, Alan D. Ashbaugh, and Ranjan Ramasamy. "A Fluorescence In Situ Hybridization (FISH) Test for Diagnosing Babesiosis." Diagnostics 10, no. 6 (June 6, 2020): 377. http://dx.doi.org/10.3390/diagnostics10060377.

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Apicomplexan parasites of the genus Babesia cause babesiosis in humans and animals. The microscopic examination of stained blood smears, detection of serum antibodies by immunoassays, and PCR-based identification of parasite nucleic acid in blood are common laboratory methods for diagnosing babesiosis. The present study evaluated a commercially available Babesia genus-specific fluorescence in situ hybridization (FISH) test for detecting Babesia parasites in blood smears. The FISH test detected Babesia duncani and Babesia microti, two common species that cause human infections in the USA, and other Babesia species of human and veterinary importance in less than two hours. The Babesia genus-specific FISH test supplements other existing laboratory methods for diagnosing babesiosis and may be particularly useful in resource-limited laboratories.
10

Shah, Jyotsna S., and Ranjan Ramasamy. "Fluorescence In Situ Hybridization (FISH) Tests for Identifying Protozoan and Bacterial Pathogens in Infectious Diseases." Diagnostics 12, no. 5 (May 21, 2022): 1286. http://dx.doi.org/10.3390/diagnostics12051286.

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Diagnosing and treating many infectious diseases depends on correctly identifying the causative pathogen. Characterization of pathogen-specific nucleic acid sequences by PCR is the most sensitive and specific method available for this purpose, although it is restricted to laboratories that have the necessary infrastructure and finance. Microscopy, rapid immunochromatographic tests for antigens, and immunoassays for detecting pathogen-specific antibodies are alternative and useful diagnostic methods with different advantages and disadvantages. Detection of ribosomal RNA molecules in the cytoplasm of bacterial and protozoan pathogens by fluorescence in-situ hybridization (FISH) using sequence-specific fluorescently labelled DNA probes, is cheaper than PCR and requires minimal equipment and infrastructure. A LED light source attached to most laboratory light microscopes can be used in place of a fluorescence microscope with a UV lamp for FISH. A FISH test hybridization can be completed in 30 min at 37 °C and the whole test in less than two hours. FISH tests can therefore be rapidly performed in both well-equipped and poorly-resourced laboratories. Highly sensitive and specific FISH tests for identifying many bacterial and protozoan pathogens that cause disease in humans, livestock and pets are reviewed, with particular reference to parasites causing malaria and babesiosis, and mycobacteria responsible for tuberculosis.
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Journal articles Dissertations / Theses Books

Dissertations / Theses on the topic "In-Situ microscopie de fluorescence":

1

Roussille, Ludovic. "Suivi quantitatif in situ d'interactions biomoléculaire par microscopie optique SEEC." Thesis, Le Mans, 2012. http://www.theses.fr/2012LEMA1030/document.

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La microscopie SEEC (Surface Enhanced Ellipsometric Contrast) est une technique inventée au mans, il y a une dizaine d’années. Elle permet de visualiser des objets de taille nanoscopique entre polariseur et analyseur croisés en utilisant les propriétés non dépolarisantes de surfaces multicouches. Jusqu’au début de la thèse, seules des observations à l’air étaient possibles. Le but de cette thèse a consisté à adapter cette technique à l’observation in-situ d’objets en immersion dans l’eau.Pour cela, il a fallu inventer de nouvelles surfaces propres à ce nouveau milieu. Les calculs ont montrés que des surfaces fines d’or révélaient un bon contraste pour des objets de 1 nm en immersion dans l’eau. Expérimentalement, nous avons montré que pour exploiter au maximum le contraste SEEC, il est nécessaire de modifier l’éclairage. En parallèle de ces travaux expérimentaux, de nouveaux calculs ont montrés que l’utilisation d’épaisseurs encore plus fines permettait de visualiser ces objets avec un bon contraste et sans aucune modification de l’éclairage. Nous avons appelé cette nouvelle technique : la microscopie CONE. Nous avons découvert deux modes de mesure. Après avoir réalisé des fonctionnalisations homogènes et hétérogènes des surfaces d’or. Ces surfaces ont été utilisées en résonance plasmonique de surface (SPR) pour l’étude de fixation de protéine (adsorption et immobilisation) puis d’interaction protéine/protéine. Ces expériences ont ensuite servies de référence pour évaluer les microscopies SEEC et CONE. Par cela, nous avons prouvé que ces microscopies présentent de forts intérêts pour la détection in-situ de protéines avec un faible coût
This thesis was supported by National Agency for Research with the project: ANR PNANO-07 SEEC. The Surface Enhanced Ellipsometric Contrast (SEEC) microscopy has invented in 2000 at Le Mans (France). This technique allows the visualization of nanoscopic object between crossed analyzer and polarizer. It’s possible if some special multilayer surfaces are used. There surfaces must have the particularity to not change the polarization of light during the reflection. Until the beginning of the project the SEEC microscopy was useful only for air observations. The goal of the thesis was to adapt this technique to observe on gold surfaces immerged in water and to compare the performance of the SEEC microscopy with Surface Plasmonique Resonance (SPR) in that configuration. The SPR is a biomolecular interaction study reference technique. SEEC microscopy lateral resolution was evaluate by fluorescence microscopy. Next, we realize two model experiments monitor in parallel by SEEC microscopy and by SPR: BSA immobilization and biotinylated IgG fixation by immobilized streptavidine. To compare measurements efficiently we did a huge preparation work (surface functionalizations and microfluidic) to have exactly same conditions in both techniques.Our results show SEEC microscopy cannot replace SPR for biomolecular interaction studies but it can be used as cheap immunological diagnostic technique. This work gives the path to follow on that direction
2

Man, Hiu Mun. "Characterisation of enzymatic catalysis by microscopy and electrochemistry : application to H2/O2 bio-fuel cells." Electronic Thesis or Diss., Aix-Marseille, 2022. http://theses.univ-amu.fr.lama.univ-amu.fr/221207_MAN_82cby815lbx134rmsegm855nh_TH.pdf.

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Les biopiles enzymatiques, qui utilisent des enzymes pour convertir l'énergie chimique en électricité, se présentent comme l'une des ressources énergétiques alternatives et propres les plus prometteuses. Cependant, l'immobilisation fonctionnelle de ces enzymes sur une électrode pour une catalyse efficace suscite encore de nombreux défis. Afin d’accéder à des informations résolues dans l'espace, il est nécessaire de coupler l'électrochimie à d’autres techniques de surface. Dans cette thèse, la microscopie de fluorescence confocale à balayage laser a été couplée à l'électrochimie pour la caractérisation de la catalyse électro-enzymatique. La principale réaction étudiée était la réaction de réduction de l'oxygène catalysée par la bilirubine oxydase de Myrothecium verrucaria. Cette réaction implique une consommation de protons couplée au transfert d'électrons. En utilisant une analyse in situ, les variations locales de pH qui se produisent à proximité de la bioélectrode pendant la catalyse enzymatique sont visualisées grâce à un fluorophore dont l’émission dépend du pH, la fluorescéine. L'activité de l'enzyme a d’abord été sondée par spectroscopie UV-vis et électrochimie. Nous avons ensuite montré que l'intensité de la fluorescence enregistrée est directement proportionnelle au courant catalytique. Les profils d'appauvrissement en protons à l’interface électrochimique dans des électrolytes tamponnés et non tamponnés ont été reconstruits, afin de déterminer l'influence de la force ionique sur l'environnement local des enzymes. Enfin, les enzymes ont été marquées avec des fluorophores, permettant de révéler les hétérogénéités locales de leur distribution interfaciale
Enzyme biofuel cells, which use enzymes to convert chemical energy into electricity, hold promise as one of the most promising alternative and clean energy resources. However, the immobilization of such enzymes on an electrode for efficient catalysis still raises many challenges. In order to access spatially resolved information, it is necessary to couple electrochemistry to other surface techniques. In this thesis, confocal laser scanning fluorescence microscopy was coupled with electrochemistry for the characterization of electro-enzymatic catalysis. The main reaction studied was the oxygen reduction reaction catalyzed by bilirubin oxidase from Myrothecium verrucaria. This reaction involves a consumption of protons coupled with electron transfer. Using in situ analysis, the local pH variations that occur near the bioelectrode during the enzymatic catalysis are visualized thanks to a fluorophore whose emission depends on the pH, fluorescein. The activity of the enzyme was first probed by UV-vis spectroscopy and electrochemistry. We then showed that the intensity of the fluorescence recorded is directly proportional to the catalytic current. Profiles of proton depletion at the electrochemical interface in buffered and unbuffered electrolytes were reconstructed to determine the influence of ionic strength on the local environment of enzymes. Finally, the enzymes were labeled with fluorophores, making it possible to reveal the local heterogeneities of their interfacial distribution
3

Mudry, Emeric. "Resolution improvement in fluorescence and phase optical microscopy." Phd thesis, Université Paul Cézanne - Aix-Marseille III, 2012. http://tel.archives-ouvertes.fr/tel-00822086.

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La microscopie optique est une technique essentielle pour de nombreuses disciplines des sciences expérimentales qui nécessitent des résolutions sans cesse plus petites. Dans ce travail de thèse sont présentés plusieurs travaux pour l'amélioration de la résolution en microscopie de fluorescence et en microscopie tomographique par diffraction (MTD), une récente technique de microscopie de phase. Dans un premier temps, il est montré que déposer l'échantillon sur un miroir permet d'augmenter la résolution axiale en MTD et en microscopie confocale de fluorescence. En microscopie confocale, il faut pour cela mettre en forme le faisceau incident grâce à un modulateur spatial de lumière. En MTD, il suffit d'adapter le programme de reconstruction. La deuxième partie présente des algorithmes pour reconstruire des images haute résolution à partir de mesures en éclairement structuré avec de champs d'illumination inconnus, à la fois en microscopie de fluorescence (algorithme blind-SIM) et en MTD. En microscopie de fluorescence, ces algorithmes permettent de simplifier drastiquement les montages expérimentaux produisant l'éclairement structuré et en MTD, d'obtenir des images d'échantillons à fort indice.
4

Milhiet, Elodie. "Nanospectroscopie de molécules d’intérêt biologique." Paris 11, 2007. http://www.theses.fr/2007PA112150.

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La spectroscopie de molécule unique joue aujourd’hui un rôle majeur dans de nombreux domaines allant de la physique fondamentale à la biologie. Dans ce contexte, mes travaux ont conduit au développement théorique et instrumental de deux méthodes d’investigation orientées vers la biologie. La première visait à caractériser la dynamique de complexation du calcium par la sonde calcique fluorescente Oregon Green Bapta5N communément employée pour l’analyse des signaux intracellulaires. Pour y parvenir, nous avons développé un dispositif expérimental de spectroscopie de corrélation de fluorescence à un et deux photons présentant une sensibilité proche de la molécule unique. Grace à ce dernier, nous avons pu étudier plusieurs aspects de la photophysique de la sonde et avons évalué ses limites ainsi que l’intérêt de l’appliquer in vivo. La seconde a consisté à développer une technique d’Hybridation In-Situ de Fluorescence (FISH) semi-quantitative afin de cartographier l’expression de gènes dans le cerveau de drosophiles adultes. Nous avons surmonté deux difficultés majeures, en obtenant, pour la première fois, des résultats reproductibles et semi-quantitatifs chez la drosophile adulte. Je présente ici une nouvelle approche où l’amplification enzymatique a été remplacée par une détection optimisée et un protocole réduisant l’impact de l’autofluorescence. Des résultats sur divers gènes exprimés dans le cerveau des drosophiles adultes y sont exposés au même titre qu’une étude visant à mieux comprendre une pathologie de retard mental. Pour conclure, j’ai mis en évidence la capacité de notre technique à résoudre des sondes uniques ce qui ouvre la voie vers de nouvelles applications
Single-molecule-like spectroscopy plays a major role in many domains, from fundamental physics to biology. In this framework, my dissertation focuses on instrumental and theoretical developments of two biological-related applications. The first experiment aims at characterizing the dynamics of calcium binding by the fluorescent calcium probe Oregon-green Bapta5N commonly employed in cell signaling analysis. To achieve it, I have developed an experimental set-up of fluorescence correlation spectroscopy that exhibits sensitivity close to that of single-molecule detection. Either monophotonic or biphotonic excitations can be used. I have investigated the several aspects of the photophysics of the probe and evaluated the interest and limitations of such an approach for future in-vivo measurements. The second one is devoted to the development of a semi-quantitative Fluorescent In-Situ Hybridization (FISH) technique for mapping gene expression in the adult drosophila brain. Two difficulties have to be solved. First, we succeeded in obtaining reproducible results with drosophila adult brain. Secondly, while most of the FISH protocols are not quantitative since they need a strong enzymatic, we achieved semi-quantitative detection of RNA probes. I will present results on a new approach for which enzymatic detection is replaced by a sensitive detection and a protocol which reduces autofluorescence contribution. Results will be presented for several genes in adult drosophila brain to validate the methods as well as an interesting application on a mental retardation disease. To conclude, I show that the method exhibits a single RNA sensitivity which opens the way to new applications
5

Lefrançois, Pauline. "Développement d’un microréacteur biomimétique pour l'analyse in situ d'activités enzymatiques par couplage de l’électrochimie et de la microscopie de fluorescence." Thesis, Bordeaux, 2017. http://www.theses.fr/2017BORD0759/document.

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De nombreuses réactions enzymatiques sont à l’origine de processus physiologiques au sein des organismes vivants. Ces réactions sont basées sur des transferts de protons et d’électrons et con-duisent souvent à la production d’espèces secondaires. Parmi elles, les espèces réactives de l’oxygène et de l’azote (ROS, RNS) présentent un intérêt particulier puisqu’elles jouent un double rôle : d’une part en permettant à l’organisme de réagir à un stress par l’activation de voie de signalisation redox, et d’autre part ces ROS et RNS peuvent causer des dommages tissulaires et être à l’origine de dys-fonctionnement (stress oxydant) au sein de l’organisme. La haute réactivité de ces espèces induit leurs faibles durées de vie (ns-min) et rend l’étude de certaines réactions enzymatiques difficiles en solu-tion. Ce projet de thèse a pour objectif de développer un microréacteur biomimétique pour l’étude d’activités enzymatiques produisant des ROS/RNS. En effet, en confinant une réaction au sein d’un compartiment de taille équivalente à celle d’une cellule (20-100 μm de diamètre), les espèces générées (H2O2, NO•, NO2-) doivent pouvoir être sondées in situ avec une résolution cinétique et quantitative. Des vésicules unilamellaires géantes sont formées en conditions physiologiques et servent de micro-réacteurs pour l’analyse des activités enzymatiques de la glucose oxydase et des NO-synthases. La microscopie de fluorescence permet l’observation des vésicules et le suivi du déclenchement de la réaction assuré par microinjection. Les espèces produites sont ensuite détectées en temps réel par électrochimie afin de déchiffrer à terme les différentes voies enzymatiques des NO-Synthases
Enzymatic reactions are involved in many physiological phenomena in living organisms. These reactions are based on protons and electrons transfers and can lead to the production of by-products. Among them, reactive oxygen and nitrogen species (ROS and RNS) are of great interest as they play a double role: on the one hand by allowing the organism to react to a stress by the activation of signaling redox pathways, and on the other hand, ROS and RNS can cause oxidative damages to tissues ensuing dysfunctions in the organism. The high reactivity of such species induce their short lifetimes (ns-min) and leads to uncertainties when it comes to the study of some enzymatic reactions in bulk. This PhD project aims to develop a biomimetic microreactor for the study of enzymatic ac-tivities producing ROS/RNS. Indeed, by confining a reaction within a cell-sized compartment (20-100 μm diameter), the generated species (H2O2, NO•, NO2-) could be analyzed in situ with a quantita-tive and kinetic resolution. Giant unilamellar vesicles are formed in physiological conditions and are used as microreactors for the monitoring of enzymatic activities of glucose oxidase and NO-synthases. Fluorescence microscopy allows individual vesicle observation and the monitoring of reactions trig-gered by microinjection. Then, released species are detected in real-time by electrochemistry in order to decipher the diverse enzymatic pathways of NO-Synthases
6

Cabriel, Clément. "Three-dimensional and multicolour approaches in super-resolution fluorescence microscopy for biology." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLS220.

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Pour analyser la structure et la dynamique des échantillons, la biologie cellulaire repose sur l'utilisation d'outils d'imagerie. En particulier, la microscopie de fluorescence offre une grande spécificité et une toxicité réduite. L'émergence récente des méthodes de super-résolution a permis d'outrepasser la limite de diffraction et ouvert de nouvelles perspectives d'études. Les stratégies de molécule unique sont particulièrement adaptées à l'imagerie nanométrique tridimensionnelle, et permettent de nombreux couplages avec des modalités complémentaires ; toutefois, leur manque de reproductibilité entrave leur généralisation.Nous proposons ici de nouvelles méthodes dans le but de remédier à ces problèmes en facilitant leur application en biologie cellulaire, en chimie et en science des matériaux. Tout d'abord, nous présentons des protocoles et échantillons dédiés aux acquisitions de calibration et de mesure de performances. Nous décrivons également plusieurs exemples d'utilisation de super-localisation tridimensionnelle dans le cadre d'études d'adhésion cellulaire et de résistance bactérienne.Ensuite, nous nous concentrons au développement d'une nouvelle méthode de microscopie de localisation de molécules uniques tri-dimensionnelle permettant l'élimination de biais de détection. Ceci est permis par le couplage entre deux stratégies complémentaires: la mise en forme de fonction d'étalement de point, et la détection de la fluorescence d'angle super-critique. L'intercorrélation et la recombinaison des informations latérales et axiales permet l'obtention d'une résolution quasi-isotrope, avec des précisions jusqu'à 15 nanomètres sur une plage de capture d'un micron. Nous mettons en évidence l'insensibilité de la méthode aux biais d'imagerie comme la dérive axiale, l'aberration chromatique et l'inclinaison de l'échantillon, et nous l'illustrons à travers des applications à la neurobiologie et au marquage de bactéries.Pour finir, nous présentons deux nouvelles approches pour le découplage d'acquisitions multi-espèces simultanées. Toutes deux basées entièrement sur le post-traitement des données acquises, elles exploitent respectivement la mesure des tailles des taches et le comportement dynamique du clignotement. Après une preuve de principe, nous évaluons l'impact des différents paramètres susceptibles d'influencer les résultats. Nous concluons en proposant des pistes d'amélioration des performances de découplage, et en suggérant de possibles couplages avec des méthodes complémentaires en imagerie de molécules uniques
Cell biology relies on imaging tools to provide structural and dynamic information about samples. Among them, fluorescence microscopy offers a compromise between high specificity and low toxicity. Recently, super-resolution methods overcame the diffraction barrier to unlock new fields of investigation. Single molecule approaches prove especially useful for three-dimensional nanoscale imaging, and allow couplings between different detection modalities. Still, their use is hindered by the complexity of the methods as well as the lack of reproducibility between experiments.We propose new methods to render super-localisation microscopy more easily applicable to relevant studies in cell biology, chemistry and material science. First, we introduce dedicated protocols and samples to eliminate sources of error in calibration and performance measurement acquisitions. We also provide examples of uses of three-dimensional super-localisation for state-of-the-art studies in the frameworks of cell adhesion and bacterial resistance to drugs.Then, we focus on the development of a novel optical method that provides unbiased results in three-dimensional single molecule localisation microscopy. This is achieved through the combination of two complementary axial detection strategies: point spread function shaping on the one hand, and supercritical angle fluorescence detection on the other hand. By cross-correlating and merging the lateral and axial positions provided by the different sources, we achieve quasi-isotropic localisation precisions down to 15 nanometres over a 1-micrometre capture range. We demonstrate the insensibility of the method to imaging non-idealities such as axial drift, chromatic aberration and sample tilt, and we propose applications in neurobiology and bacteria labelling.Finally, we introduce two new post-processing approaches for the demixing of simultaneous multi-species acquisitions. They are based respectively on the measurement of the spot sizes, and on the assessment of the dynamic blinking behaviour of molecules. After demonstrating a proof of principle, we assess the impact of the different parameters likely to influence the results. Eventually, we discuss leads to improve the demixing performances, and we discuss the coupling possibilities with complementary single molecule localisation techniques
7

Wang, Ruixing. "STED-fluorescence correlation spectroscopy for dynamic observations in cell biology : from theoretical to practical approaches." Thesis, Aix-Marseille, 2018. http://www.theses.fr/2018AIXM0163/document.

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Les techniques de super-résolution offrent un nouvel aperçu de la description de l'organisation moléculaire dynamique de la membrane plasmique. Parmi ces techniques, la microscopie par déplétion d'émission stimulée (stimulated emission depletion, STED) dépasse la limite de diffraction optique et atteint une résolution de quelques dizaines de nanomètres. Il est une technique polyvalente qui peut être combinée avec d'autres techniques telles que la spectroscopie par corrélation de fluorescence (fluorescence correlation spectroscopy, FCS), fournissant des résolutions spatiales et temporelles élevées pour explorer les processus dynamiques qui se produisent dans les cellules vivantes. Ce projet de doctorat vise à mettre en œuvre un microscope STED, puis à combiner ce module STED avec la technique FCS pour les applications biologiques. Des études théoriques du STED et de la technique combinant STED et FCS ont permis dans les aspects spatio-temporels. Une solution analytique pour la fonction d'autocorrélation FCS a été dérivée dans l'état de déplétion STED incomplet. et un nouveau modèle d'ajustement FCS a été proposé. La méthode de variation du volume d’observation FCS (spot variation FCS, svFCS) a démontré sa capacité à identifier la présence de nanodomaines limitant la diffusion latérale des molécules dans la membrane plasmique. L’approche STED-FCS permet d’étendre l’application de la svFCS à l'échelle nanométrique afin d’évaluer la persistance plus ou moins importante de tels nanodomaines. Dans ce contexte, des simulations préliminaires de Monte Carlo ont été réalisées figurant des molécules diffusant en présence d'auto-assemblage/désassemblage dynamique des nanodomaines
Super-resolution techniques offer new insight into the description of the dynamic molecular organization at the plasma membrane. Among these techniques, the stimulated emission depletion (STED) microscopy breaks the optical diffraction limit and reaches the resolution of tens of nanometer. It is a versatile setup that can be combined with other techniques such as fluorescence correlation spectroscopy (FCS), providing both high spatial and temporal resolutions to explore dynamic processes occurring in live cells. This PhD project aims at implementing a STED microscope, and then at combining this STED module with FCS technique for biological applications. Detailed theoretical studies on STED and the combined STED-FCS technique in spatio-temporal aspects were performed. An analytical solution for FCS autocorrelation function was derived in the condition of incomplete STED depletion and a new FCS fitting model was proposed to overcome this problem. The spot variation FCS (svFCS) method has demonstrated its capability to identify the presence of nanodomains constraining the lateral diffusion of molecules at the plasma membrane. The STED-FCS can extend the svFCS approach to the nanoscale evaluating the long-lasting existence of such nanodomains. Within this frame, preliminary Monte Carlo simulations were conducted mimicking molecules diffusing in the presence of dynamic self-assembling/disassembling nanodomains
8

Galanti, Agostino. "Multi-photochromic architectures : from structure to function." Thesis, Strasbourg, 2018. http://www.theses.fr/2018STRAF046/document.

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L’objectif de cette thèse a été axé sur le développement des systèmes capable de répondre à des stimuli externes, basés sur des unités photochromiques. Le but d’une telle quête est d’augmenter la complexité des dispositifs et des machines moléculaires synthétiques. Avec l’objectif de développer des dispositifs et des machines artificiels plus complexes, nous avons réalisé de systèmes comprenant de multiples interrupteurs moléculaires. En vue de la réalisation de cette thèse, des nouveaux systèmes multi-photochromiques, où hybrides photochrome/nanomatériaux contenant des fragments azobenzène, diaryléthène ou spiropyrane ont été réalisés et étudiés. D’abord, on s’est focalisés sur des systèmes multi-azobenzènes capables de subir de grands réarrangements géométriques lors de la photoisomérisation, ils pourraient être utilisés à l'avenir comme éléments constitutifs des matériaux host-guest ou metal-organic frameworks contrôlables par des stimuli lumineux. Dans un second exemple, des commutateurs photochromiques de type dithiényléthène ont été utilisés pour déclencher l'émission d'une porphyrine. Cette dyade à montré une modulation réversible de son émission, affichant un contraste particulièrement élevé. Comme dernier exemple, un dérivé de spiropyrane a été combiné avec des nanoparticules d’or anisotropes. En induisant l'isomérisation de l’interrupteur moléculaire dans les dispersions colloïdales des nanorods d’or en liquide, nous avons visualisé une grande variation du spectre d'extinction des colloïdes, dépendante de la longueur d’onde du mode LSPR et du recouvrement spectrale avec le photoswitch
The aim of this thesis has been to develop systems capable of responding to external stimuli, based on photochromic units. The goal of such a quest is to increase the complexity of devices and synthetic molecular machines. With the goal of developing more complex artificial devices and machines, we have realised systems containing multiple molecular switches. For the realisation of this thesis, new multi-photochromic systems, or photochromes/nanomaterials hybrids containing azobenzene, diarylethene or spiropyran moieties have been realised and studied. Firstly, we focused on multi-azobenzene systems capable of undergoing large geometric rearrangements during photoisomerisation, as they may be used in the future as constituent elements of host-guest or metal-organic frameworks controllable by luminous stimuli. In a second example, dithienylethene-type photochromic switches have been used to trigger the emission of a porphyrin. This dyad exhibited a reversible modulation of its emission, displaying a particularly highly contrasted response. As a final example, a spiropyran derivative has been combined with anisotropic gold nanoparticles. By inducing the isomerisation of the molecular switch in the AuNR colloidal liquid dispersions, we visualised a large variation of the colloid extinction spectrum, dependent on the LSPR mode wavelength and the spectral overlap with the photoswitch
9

Zheng, Zheng. "Development of Far-Red / Near-Infrared Luminescent Chromophores and Nanoparticles for in vivo Biphotonic Applications." Thesis, Lyon, 2016. http://www.theses.fr/2016LYSEN024/document.

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Développer de nouveaux fluorophores ayant une forte section efficace d’absorption à deux photons (ADP) et des propriétés d'émission dans le rouge lointain est important, en particulier pour l’imagerie in-vivo profonde. Cette gamme de longueur d'onde correspond en effet à la fenêtre de transparence optique des tissus. Cette thèse étudie le potentiel de nouveaux fluorophores émettant dans rouge construits sur un noyau fluorène pour la microscopie à deux photons in-vivo, en privilégiant l'imagerie du système vasculaire, d'une part, et la mesure optique de la pression d'oxygène dissous, d'autre part.Ainsi, une famille de chromophores asymétriques a été conçue et synthétisée. La plupart des chromophores présentent une forte émission dans le proche IR, induite par l'agrégation. De plus, une stratégie de co-protection basée sur un système micellaire / silice a été utilisé pour préparer des nanoparticules avec un intérieur apolaire et conserver les propriétés optiques des chromophores dipolaires en solution aqueuse. Des mesures de fluorescence excitée à deux photons ont été menées en solvant organique et en suspension aqueuse. Les agrégats et les nanoparticules ont été utilisés avec succès en imagerie biphotonique du système vasculaire sur petit animal en utilisant un modèle de tumeur à l'intérieur de la peau de l'oreille de la souris. Les nanoparticules de silice montrent une coloration exceptionnelle du système vasculaire qui en fait de parfaits marqueurs du système vasculaire.Dans un deuxième temps, quatre nouveaux chromophores, absorbant à deux photons, ont été synthétisés et leurs propriétés photo physiques à un et à deux photons ont été étudiées. Le chromophore le plus adapté a ensuite été greffé de manière covalente par chimie click, à un complexe de palladium avec un ligand porphyrine, cœur phosphorescent dont l’émission est sensible à la présence d’oxygène. Deux composés contenant quatre ou huit absorbeur à deux photons ont été obtenus et étudiés. Les résultats démontrent que l'incorporation d'un chromophore ADP approprié peut effectivement augmenter les propriétés d’ADP du système, ce qui permet une sensibilité efficace vis-à-vis de l'oxygène
The development of fluorophores with efficient two-photon absorption (TPA) and emission properties in the far red/NIR is important, especially for in depth in-vivo optical imaging as this wavelength range corresponds to the optical transparency window of tissues. This thesis investigates the potential of new red emitting fluorophores containing a fluorene ring for in-vivo two-photon microscopy focusing on vascular imaging on one hand and on oxygen pressure measurement on the other hand.A new series of asymmetrical fluorene-based chromophores were designed and synthesized. Their structure-property relationships were systematically investigated. It was found that most of chromophores exhibit aggregation-induced emission behaviors in the NIR region. In addition, a micelle/silica coprotection strategy was proposed to prepare nanoparticles with a less polar interior, which can be used to conserve optical properties of dipole chromophores in aqueous solution. The two-photon excited fluorescence (TPEF) measurements indicate that they all display obvious TPA activities in organic solvent and aqueous suspension. Both the NIR-emissive aggregates and nanoparticles have been successfully used for TPEF imaging of blood vessels inside mouse ear skin. The silica nanoparticles show outstanding staining of the vascular system making them perfect blood pool markers.On a second part, four new fluorene-based two-photon absorbing chromophores have been synthesized and their one- and two-photon photophysical properties were investigated. The optimum chromophore was successfully attached covalently to an oxygen responsive phosphorescent Pd-porphyrin complex by click chemistry. Two new compounds contain four or eight TPA chromophores donor connected to the phosphorescent core. The result demonstrate that the incorporation of a suitable TPA chromophore can effectively enhance the TPA of the system, allowing efficient sensitivity towards oxygen
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Gasecka, Alicja. "Polarimetric multiphoton fluorescence microscopy in molecular and biological media." Aix-Marseille 3, 2010. http://www.theses.fr/2010AIX30068.

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Les interactions lumière-matière dans les mileux moléculaires et bio-moléculaires peuvent mener à des processus complexes où les polarisations des champs optiques se couplent aux assemblages de dipoles de transitions moléculaires. La manipulation des polarisations des champs optiques en microscopie de fluorescence peut en particulier donner accès à des modifications fines d'arrangements moléculaires. Dans ce travail de thèse nous introduisons une méthode basée sur la variation continue d'un état de polarisation d'excitation complémentée par une analyse polarisée, appliquée à la microscopie de fluorescence multi-photons. La fluorescence à deux photons polarimétrique permet d'accéder à une information statique quantitative sur la forme et l'orientation de la distribution orientationnelle moléculaire dans des membranes lipidiques artificielles, dans des cellules ou sur des composés molécluaires co-cristallins qui peuvent être fortement hétérogènes. La fluorescence à trois photons polarimétrique apporte de plus un diagnostique de cristallinité dans des cristaux de protéines, avec une forte sensibilité à leur structure et symétrie. L'implémentation expérimentale de cette technique requiert de quantifier les distortions de polarisation provenant du montage expérimental et de l'échantillon lui-même, qui sont finement analysés
Light-matter interaction in molecular and bio-molecular media can lead to complex processes where optical fields polarizations couple to an assembly of molecular transition dipoles. The manipulation of the optical fields polarization in fluorescence microscopy can in particular give access to fine changes occurring in molecular arrangements. In this PhD thesis we report a method based on a tuneable excitation polarization state complemented by a polarized read-out, applied to polarization-resolved multiphoton fluorescence microscopy. Two-photon fluorescence polarimetry allows to retrieve a quantitative information on the static molecular distribution shape and orientation in different environments such as model lipid membranes, cell membranes, and molecular inclusion compounds that can be strongly heterogeneous. Three-photon fluorescence polarimetry has been furthermore applied in bio-molecular media in order to provide a diagnostics for crystallinity in protein crystals with high sensitivity to their structure and symmetry. The experimental implementation of polarimetric multi-photon microscopy requires to quantify possible polarization distortions originating from the experimental set-up or sample itself, which are thoroughly analyzed
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Books on the topic "In-Situ microscopie de fluorescence":

1

Liehr, Thomas. Fluorescence In Situ Hybridization (FISH) — Application Guide. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009.

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Bridger, Joanna M., and Emanuela V. Volpi. Fluorescence in situ hybridization (FISH): Protocols and applications. New York, NY: Humana Press, 2010.

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Ammasi, Periasamy, and Clegg Robert M, eds. FLIM microscopy in biology and medicine. Boca Raton: Taylor & Francis, 2009.

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1923-, Sharma Arun Kumar, and Sharma Archana 1932-, eds. Chromosome painting: Principles, strategies, and scope. Dordrecht: Kluwer Academic Publishers, 2001.

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Fried, Alexander. Studies of phase transitions in supported lipid bilayers by simultaneous in situ fluorescence spectroscopy and atomic force microscopy. Ottawa: National Library of Canada, 2002.

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A, Sharif N., ed. Molecular imaging in neuroscience: A practical approach. Oxford [England]: IRL Press at Oxford University Press, 1993.

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Bridger, Joanna M., and Emanuela V. Volpi, eds. Fluorescence in situ Hybridization (FISH). Totowa, NJ: Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-789-1.

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Liehr, Thomas, ed. Fluorescence In Situ Hybridization (FISH). Berlin, Heidelberg: Springer Berlin Heidelberg, 2017. http://dx.doi.org/10.1007/978-3-662-52959-1.

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Haimovich, Gal, ed. Fluorescence In Situ Hybridization (FISH). New York, NY: Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3766-1.

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Morel, Gérard. Hybridation in situ en microscopie électronique. Paris: Technique & Documentation, 2001.

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Book chapters on the topic "In-Situ microscopie de fluorescence":

1

Michalová, Kyra, Zuzana Zemanová, Jana Březinová, and Věra Michalová. "Fluorescence in Situ Hybridization (FISH) in Cytogenetics of Leukemia." In Fluorescence Microscopy and Fluorescent Probes, 185–89. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_27.

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Rinke, Bernd, Joachim Bradl, Bernhard Schneider, Markus Durm, Michael Hausmann, Horst Ludwig, and Christoph Cremer. "“In Situ” Estimates of the Spatial Resolution for “Practical” Fluorescence Microscopy of Cell Nuclei." In Fluorescence Microscopy and Fluorescent Probes, 169–73. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4899-1866-6_24.

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Iourov, Ivan Y. "Microscopy and Imaging Systems." In Fluorescence In Situ Hybridization (FISH) — Application Guide, 75–84. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-70581-9_7.

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Inácio, João, and Maria da Luz Martins. "Microscopic Detection of Yeasts Using Fluorescence In Situ Hybridization." In Methods in Molecular Biology, 71–82. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-257-5_5.

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Figueiredo, Joana, Ana Sofia Ribeiro, Tânia Mestre, Sofia Esménio, Martina Fonseca, Joana Paredes, Raquel Seruca, and João M. Sanches. "Capturing quantitative features of protein expression from in situ fluorescence microscopic images of cancer cell populations." In Fluorescence Imaging and Biological Quantification, 279–97. Boca Raton : Taylor & Francis, 2017.: CRC Press, 2017. http://dx.doi.org/10.1201/9781315121017-15.

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Edwards, Matthew S. "Using in situ substratum sterilization and fluorescence microscopy in studies of microscopic stages of marine macroalgae." In Sixteenth International Seaweed Symposium, 253–59. Dordrecht: Springer Netherlands, 1999. http://dx.doi.org/10.1007/978-94-011-4449-0_29.

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Markaki, Yolanda, Daniel Smeets, Marion Cremer, and Lothar Schermelleh. "Fluorescence In Situ Hybridization Applications for Super-Resolution 3D Structured Illumination Microscopy." In Nanoimaging, 43–64. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-137-0_4.

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Smolka, John A., and Samantha C. Lewis. "In Situ Analysis of Mitochondrial DNA Synthesis Using Metabolic Labeling Coupled to Fluorescence Microscopy." In Methods in Molecular Biology, 99–106. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2922-2_8.

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Loseva, Elizaveta, Jaap van Krugten, Aniruddha Mitra, and Erwin J. G. Peterman. "Single-Molecule Fluorescence Microscopy in Sensory Cilia of Living Caenorhabditis elegans." In Single Molecule Analysis, 133–50. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3377-9_7.

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AbstractIntracellular transport of organelles and biomolecules is vital for several cellular processes. Single-molecule fluorescence microscopy can illuminate molecular aspects of the dynamics of individual biomolecules that remain unresolved in ensemble experiments. For example, studying single-molecule trajectories of moving biomolecules can reveal motility properties such as velocity, diffusivity, location and duration of pauses, etc. We use single-molecule imaging to study the dynamics of microtubule-based motor proteins and their cargo in the primary cilia of living C. elegans. To this end, we employ standard fluorescent proteins, an epi-illuminated, widefield fluorescence microscope, and primarily open-source software. This chapter describes the setup we use, the preparation of samples, a protocol for single-molecule imaging in primary cilia of C. elegans, and data analysis.
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Casanova-Moreno, Jannu, Zhinan Landis Yu, Jonathan Massey-Allard, Brian Ditchburn, Jeff F. Young, and Dan Bizzotto. "In Situ Spectroelectrochemical Fluorescence Microscopy for Visualizing Interfacial Structure and Dynamics in Self-assembled Monolayers." In Luminescence in Electrochemistry, 21–77. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-49137-0_2.

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Conference papers on the topic "In-Situ microscopie de fluorescence":

1

Samuel, B. A., and M. A. Haque. "In-Situ Nanoscale Single Fiber Fragmentation Using Fluorescence Microscopy." In ASME 2007 International Mechanical Engineering Congress and Exposition. ASMEDC, 2007. http://dx.doi.org/10.1115/imece2007-43367.

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We use fluorescence microscopy to perform real time single fiber fragmentation experiments on individual PolyFurfuryl Alcohol (PFA) nanofibers embedded within a Poly DiMethyl Siloxane (PDMS) matrix. By using fluorescent nanofibers in an optically transparent matrix, fragmentation of the nanoscale fibers (even less than 400 nm) can also be easily visualized using fluorescence emission from the nanowires. When the composite specimen was loaded to saturation strain the fragment lengths distribution was observed to follow a two parameter Weibull frequency distribution. In addition, we also present a digital image correlation based technique to obtain localized strain (and hence stress) data based on the use of fluorescent nanoscale spatial markers.
2

Son, Jeonghwan, Biagio Mandracchia, and Shu Jia. "Miniaturized optical fluorescence microscopy system for parallel in situ imaging." In Frontiers in Optics. Washington, D.C.: OSA, 2020. http://dx.doi.org/10.1364/fio.2020.fw5e.2.

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Kung, C.-Y., M. D. Barnes, N. Lermer, W. B. Whitten, and J. M. Ramsey. "Confinement, Detection, and Manipulation of Individual Molecules in Attoliter Volumes." In Laser Applications to Chemical and Environmental Analysis. Washington, D.C.: Optica Publishing Group, 1998. http://dx.doi.org/10.1364/lacea.1998.lma.4.

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We report observation of fluorescence from individual rhodamine 6G molecules in streams of charged 1-μm diameter water droplets. With this approach, probe volumes comparable to diffraction-limited fluorescence microscopy1 techniques (≤ 500 attoliters) are achieved, resulting in similarly high contrast between single molecule fluorescence signals and nonfluorescent background. However, since the fluorescent molecules are confined to electrically charged droplets, in situ electrodynamic manipulation can be accomplished in a straightforward manner, allowing experimental control over both the delivery of molecules of interest to the observation region and the laser-molecule interaction time.
4

Dursun, Gizem, and Ufuk Ozkaya. "Microscopic Fluorescence In Situ Hybridization (FISH) Image Synthesis with Generative Adversarial Networks." In 2021 29th Signal Processing and Communications Applications Conference (SIU). IEEE, 2021. http://dx.doi.org/10.1109/siu53274.2021.9477999.

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Qu, Min, and Yanming Zhang. "The study on improving fluorescence microscopy image effects of genomic in situ hybridization." In 2010 3rd International Conference on Biomedical Engineering and Informatics (BMEI). IEEE, 2010. http://dx.doi.org/10.1109/bmei.2010.5640551.

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Bai, Yeran, Zhongyue Guo, ‪Fátima Pereira, Michael Wagner, and Ji-Xin Cheng. "Microbial identification and metabolic analysis by mid-infrared photothermal imaging fluorescence in-situ hybridization." In Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, edited by Garth J. Simpson, Ji-Xin Cheng, and Wei Min. SPIE, 2022. http://dx.doi.org/10.1117/12.2611781.

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Klimas, Aleksandra, Brendan R. Gallagher, Emma DiBernardo, Zhangyu Cheng, and Yongxin Zhao. "MAGNIFY: molecule anchorable gel-enabled nanoscale in-situ fluorescence microscopy for nanoscale imaging of biomolecules." In Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX, edited by Thomas G. Brown, Tony Wilson, and Laura Waller. SPIE, 2023. http://dx.doi.org/10.1117/12.2647983.

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Kubarev, Alexey. "Development of the in-situ fluorescence microscopy approach to reveal the mechanism of interfacial polymerization of polyamide membranes." In European Microscopy Congress 2020. Royal Microscopical Society, 2021. http://dx.doi.org/10.22443/rms.emc2020.606.

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Serabyn, Gene, Kurt Liewer, Chris Lindensmith, Kent Wallace, and Jay Nadeau. "Development of a light-field fluorescence microscope for in situ life searches in the solar system." In 2019 IEEE Aerospace Conference. IEEE, 2019. http://dx.doi.org/10.1109/aero.2019.8741627.

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Kabakci, Kaan A., Abdulkerim Capar, B. Ugur Toreyin, Mertkan Akkoc, Ozan Borazan, Ilknur Turkmen, and Lutfiye Durak Ata. "A multi-level thresholding based segmentation method for microscopic fluorescence in situ hybridization (FISH) images." In 2016 24th Signal Processing and Communication Application Conference (SIU). IEEE, 2016. http://dx.doi.org/10.1109/siu.2016.7495873.

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Reports on the topic "In-Situ microscopie de fluorescence":

1

Korenberg, J. R. Human cDNA mapping using fluorescence in situ hybridization. Office of Scientific and Technical Information (OSTI), March 1993. http://dx.doi.org/10.2172/6659571.

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2

Falkner, Kelly K. Development of a Fluorescence-Based in-situ Barium Sensor. Fort Belvoir, VA: Defense Technical Information Center, October 1999. http://dx.doi.org/10.21236/ada375880.

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3

Lucas, J. N., and T. Straume. Chromosome translocations measured by fluorescence in-situ hybridization: A promising biomarker. Office of Scientific and Technical Information (OSTI), October 1995. http://dx.doi.org/10.2172/132690.

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4

Knowles, David S., Stephen H. Lieberman, Michele Davey, and Blair Wingfield. Laser Induced Fluorescence Spectroscopy for in-situ Detection of Petroleum Contamination. Fort Belvoir, VA: Defense Technical Information Center, January 1996. http://dx.doi.org/10.21236/ada348854.

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5

Bur, A. J., F. W. Wang, A. Lee, R. E. Lowry, S. C. Roth, and T. K. Trout. In SITU fluorescence monitoring of the viscosities of particle-filled polymers in flow. Gaithersburg, MD: National Bureau of Standards, 1988. http://dx.doi.org/10.6028/nist.ir.88-3892.

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Bur, Anthony J., Francis W. Wang, and Ronald E. Dehl. In situ fluorescence monitoring of the viscosities of particle-filled polymers in flow. Gaithersburg, MD: National Bureau of Standards, 1988. http://dx.doi.org/10.6028/nbs.ir.88-3694.

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7

Mazel, Charles. In Situ Spectral Properties (Reflectance and Fluorescence) of Benthic Substrates and Organisms. Fort Belvoir, VA: Defense Technical Information Center, September 1997. http://dx.doi.org/10.21236/ada628810.

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8

Sharpe, Taylor. Assessing a Fluorescence Spectroscopy Method for In-Situ Microbial Drinking Water Quality. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.5732.

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9

Dean, Jay B. Hyperbaric Imaging Equipment: Fluorescence Microscopy for In Vitro Studies of Oxygen Toxicity. Fort Belvoir, VA: Defense Technical Information Center, August 2004. http://dx.doi.org/10.21236/ada425309.

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10

Mazel, Charles. A Multispectral Fluorescence and Reflectance Probe for In Situ Characterization of Benthic Environments. Fort Belvoir, VA: Defense Technical Information Center, September 1999. http://dx.doi.org/10.21236/ada630464.

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