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Journal articles on the topic 'In-situ hybridisation'

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Published: 4 June 2021
Last updated: 1 February 2023

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1

Herrington, C. S. "Demystified ... in situ hybridisation." Molecular Pathology 51, no. 1 (February 1, 1998): 8–13. http://dx.doi.org/10.1136/mp.51.1.8.

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2

Cassidy, Andrew, and Julia Jones. "Developments in in situ hybridisation." Methods 70, no. 1 (November 2014): 39–45. http://dx.doi.org/10.1016/j.ymeth.2014.04.006.

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3

Warford, A., and I. Lauder. "In situ hybridisation in perspective." Journal of Clinical Pathology 44, no. 3 (March 1, 1991): 177–81. http://dx.doi.org/10.1136/jcp.44.3.177.

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4

Ekong, Rosemary, and Jonathan Wolfe. "Advances in fluorescent in situ hybridisation." Current Opinion in Biotechnology 9, no. 1 (February 1998): 19–24. http://dx.doi.org/10.1016/s0958-1669(98)80079-7.

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5

Chiecchio, Laura. "In situ hybridisation in tissue sections." Diagnostic Histopathology 26, no. 11 (November 2020): 521–28. http://dx.doi.org/10.1016/j.mpdhp.2020.08.005.

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6

Edwards, A. A. "Editorial - Fluorescence In Situ Hybridisation (FISH)." Radiation Protection Dosimetry 88, no. 1 (March 1, 2000): 5–6. http://dx.doi.org/10.1093/oxfordjournals.rpd.a033019.

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7

Morey, Adrienne L. "In situ hybridisation: background and applications." Pathology 43 (2011): S10. http://dx.doi.org/10.1016/s0031-3025(16)33100-2.

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8

Terenghi, Giorgio, and Julia M. Polak. "Morphological studies using in situ hybridisation." European Journal of Cancer and Clinical Oncology 27, no. 6 (June 1991): 785–89. http://dx.doi.org/10.1016/0277-5379(91)90190-o.

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9

Yao, Jin, Xingmei Li, Na Wu, Songlin Zhang, Min Gao, and Xiping Wang. "Improvement of RNA In Situ Hybridisation for Grapevine Fruits and Ovules." International Journal of Molecular Sciences 24, no. 1 (January 2, 2023): 800. http://dx.doi.org/10.3390/ijms24010800.

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The European grapevine (Vitis vinifera L.) is one of the world’s most widely cultivated and economically important fruit crops. Seedless fruits are particularly desired for table grapes, with seedlessness resulting from stenospermocarpy being an important goal for cultivar improvement. The establishment of an RNA in situ hybridisation (ISH) system for grape berries and ovules is, therefore, important for understanding the molecular mechanisms of ovule abortion in stenospermocarpic seedless cultivars. We improved RNA in situ hybridisation procedures for developing berries and ovules by targeting two transcription factor genes, VvHB63 and VvTAU, using two seeded varieties, ‘Red Globe’ and ‘Pinot Noir’, and two seedless cultivars, ‘Flame Seedless’ and ‘Thompson Seedless’. Optimisation focused on the time of proteinase K treatment, probe length, probe concentration, hybridisation temperature and post-hybridisation washing conditions. The objectives were to maximise hybridisation signals and minimise background interference, while still preserving tissue integrity. For the target genes and samples tested, the best results were obtained with a pre-hybridisation proteinase K treatment of 30 min, probe length of 150 bp and concentration of 100 ng/mL, hybridisation temperature of 50 °C, three washes with 0.2× saline sodium citrate (SSC) solution and blocking with 1% blocking reagent for 45 min during the subsequent hybridisation. The improved ISH system was used to study the spatiotemporal expression patterns of genes related to ovule development at a microscopic level.
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10

Shastry, Anjali, Preetha Tilak, and Amudha Subramaniam. "APPLICATION OF KARYOTYPING AND FLOURESCENT IN SITU HYBRIDISATION IN DETECTION OF KLINEFELTER SYNDROME." International Journal of Anatomy and Research 6, no. 3.3 (September 5, 2018): 5682–85. http://dx.doi.org/10.16965/ijar.2018.310.

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11

Briscioli, V., G. Floridia, E. Rossi, A. Selicorni, F. Lalatta, and O. Zuffardi. "Trisomy 10qter confirmed by in situ hybridisation." Journal of Medical Genetics 30, no. 7 (July 1, 1993): 601–3. http://dx.doi.org/10.1136/jmg.30.7.601.

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12

Seres-Santamaria, A., V. Catala, E. Cuatrecasas, and R. Villanueva. "Fluorescent in-situ hybridisation and Down's syndrome." Lancet 341, no. 8859 (June 1993): 1544. http://dx.doi.org/10.1016/0140-6736(93)90690-i.

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13

Bryant, Stewart. "Medicare funding for fluorescence in situ hybridisation." Pathology 39, no. 5 (October 2007): 535. http://dx.doi.org/10.1080/00313020701621369.

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14

Lerner, Boaz. "Bayesian fluorescence in situ hybridisation signal classification." Artificial Intelligence in Medicine 30, no. 3 (March 2004): 301–16. http://dx.doi.org/10.1016/j.artmed.2003.11.005.

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15

Gill, Kamalvir, Jun Sasaki, Parul Jayakar, Lisa Sosa, and Elizabeth Welch. "Chromosomal microarray detects genetic risks of neurodevelopmental disorders in newborns with congenital heart disease." Cardiology in the Young 31, no. 8 (February 4, 2021): 1275–82. http://dx.doi.org/10.1017/s1047951121000202.

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AbstractObjective:To compare the genetic testing results of neonates with CHD by chromosomal microarray to karyotyping and fluorescence in situ hybridisation analysis.Methods:This was a single-centre retrospective comparative study of patients with CHD and available genetic testing results admitted to the cardiac ICU between January, 2004 and December, 2017. Patients from 2004 to 2010 were tested by karyotyping and fluorescence in situ hybridisation analysis, while patients from 2012 to 2017 were analysed by chromosomal microarray.Results:Eight-hundred and forty-nine neonates with CHD underwent genetic testing, 482 by karyotyping and fluorescence in situ hybridization, and 367 by chromosomal microarray. In the karyotyping and fluorescence in situ hybridisation analysis group, 86/482 (17.8%) had genetic abnormalities detected, while in the chromosomal microarray group, 135/367 (36.8%) had genetic abnormalities detected (p < 0.00001). Of patients with abnormal chromosomal microarray results, 41/135 (30.4%) had genetic abnormality associated with neurodevelopmental disorders that were exclusively identified by chromosomal microarray. Conotruncal abnormalities were the most common diagnosis in both groups, with karyotyping and fluorescence in situ hybridisation analysis detecting genetic abnormalities in 26/160 (16.3%) patients and chromosomal microarray detecting abnormalities in 41/135 (30.4%) patients (p = 0.004). In patients with d-transposition of the great arteries, 0/68 (0%) were found to have genetic abnormalities by karyotyping and fluorescence in situ hybridisation compared to 7/54 (13.0%) by chromosomal microarray.Conclusions:Chromosomal microarray identified patients with CHD at genetic risk of neurodevelopmental disorders, allowing earlier intervention with multidisciplinary care and more accurate pre-surgical prognostic counselling.
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16

Harrison, R. H., H. C. Kuo, P. N. Scriven, A. H. Handyside, and C. Mackie Ogilvie. "Lack of cell cycle checkpoints in human cleavage stage embryos revealed by a clonal pattern of chromosomal mosaicism analysed by sequential multicolour FISH." Zygote 8, no. 3 (August 2000): 217–24. http://dx.doi.org/10.1017/s0967199400001015.

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Multicolour fluorescence in situ hybridisation (FISH) analysis of interphase nuclei in cleavage stage human embryos has highlighted a high incidence of postzygotic chromosomal mosaicism, including both aneuploid and ploidy mosaicism. Indeed, some embryos appear to have a chaotic chromosomal complement in a majority of nuclei, suggesting that cell cycle checkpoints may not operate in early cleavage. Most of these studies, however, have only analysed a limited number of chromosomes (3–5), making it difficult to distinguish FISH artefacts from true aneuploidy. We now report analysis of 11 chromosomes in five sequential hybridisations with standard combinations of two or three probes and minimal loss of hybridisation efficiency. Analysis of a series of arrested human embryos revealed a generally consistent pattern of hybridisation on which was superimposed frequent deletion of one or both chromosomes of a specific pair in two or more nuclei indicating a clonal origin and continued cleavage following chromosome loss. With a binucleate cell in a predominantly triploid XXX embryo, the two nuclei remained attached during preparation and the chaotic diploid/triphoid status of every chromosome analysed was the same for each nucleus. Furthermore, in each hybridisation the signals were distributed as a mirror-image about the plane of attachment, indicating premature decondensation during anaphase consistent with a lack of checkpoint control.
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17

Hulten, M. A., C. P. Gould, A. S. Goldman, and J. J. Waters. "Chromosome in situ suppression hybridisation in clinical cytogenetics." Journal of Medical Genetics 28, no. 9 (September 1, 1991): 577–82. http://dx.doi.org/10.1136/jmg.28.9.577.

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18

Campbell, Lynda J. "Fluorescence in situ hybridisation in acute myeloid leukaemia." Pathology 42 (2010): S37. http://dx.doi.org/10.1097/01268031-201042001-00072.

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19

Fleming, K. A. "In-situ hybridisation—a role in clinical pathology." Journal of Pathology 153, no. 3 (November 1987): 201–2. http://dx.doi.org/10.1002/path.1711530303.

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20

Wolf, Mirela. "Biofilm biodiversity presented by fluorescent in situ hybridisation." E3S Web of Conferences 17 (2017): 00098. http://dx.doi.org/10.1051/e3sconf/20171700098.

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21

Ward, Samantha J., Katherine Karakoula, Kim P. Phipps, William Harkness, Richard Hayward, Dominic Thompson, Thomas S. Jacques, et al. "Cytogenetic analysis of paediatric astrocytoma using comparative genomic hybridisation and fluorescence in-situ hybridisation." Journal of Neuro-Oncology 98, no. 3 (January 6, 2010): 305–18. http://dx.doi.org/10.1007/s11060-009-0081-4.

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22

Goldman, A. S., and M. A. Hulten. "Chromosome in situ suppression hybridisation in human male meiosis." Journal of Medical Genetics 29, no. 2 (February 1, 1992): 98–102. http://dx.doi.org/10.1136/jmg.29.2.98.

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23

Navikas, V., J. Link, W. Palasik, S. Fredrikson, Å. Ljundahl, B. Höjeberg, T. Olsson, and H. Link. "Cytokines in myasthenia gravis: an in situ hybridisation study." Neuromuscular Disorders 4, no. 5-6 (September 1994): S42. http://dx.doi.org/10.1016/0960-8966(94)90175-9.

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24

Schedle, A., M. Willheim, A. Zeitelberger, A. Gessl, K. Frauendorfer, C. Sch�fer, F. Wachtler, H. G. Schwarzacher, and G. Boltz-Nitulescu. "Nucleolar morphology and rDNA in situ hybridisation in monocytes." Cell & Tissue Research 269, no. 3 (September 1992): 473–80. http://dx.doi.org/10.1007/bf00353902.

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25

Tholouli, Eleni, Dolores Di Vizio, Fionnuala O’Connell, Massimo Loda, David Twomey, Todd Golub, Richard Levenson, Judith A. Hoyland, John A. L. Yin, and Richard Byers. "Quantum Dot Based Duplex In Situ Hybridisation for Gene Expression Profiling." Blood 106, no. 11 (November 16, 2005): 3265. http://dx.doi.org/10.1182/blood.v106.11.3265.3265.

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Abstract Quantum dots (QDs) are fluorescent semiconductor nanocrystals (2–10-nm core diameter) possessing the unique properties of extremely high fluorescence efficiency, lack of photobleaching and long fluorescence lifetime, making them an ideal tool for bioimaging. We have developed a novel technique for in situ hybridisation (ISH) using biotinylated oligonucleotides conjugated to streptavidin coated QD, and used them in this study to label bone marrow trephine samples. 50-mer long oligonucleotide probes were conjugated to QDs prior to ISH and conjugation efficiency was demonstrated by gel electrophopresis. ISH conditions and molar ratio of QDs to probe were optimised using a polyT probe. Images were captured using a CRI Nuance spectral imaging system and signal intensity was semi-quantitated using IPLab software. Specific oligonucleotide hybridisation was demonstrated using a probe for myeloperoxidase (MPO) in 40 bone marrow sections infiltrated by acute myeloid leukaemia (AML), acute lymphoblastic leukaemia (ALL), chronic myeloid leukaemia (CML) as well as reactive bone marrow. In each case hybridisation signal was consistent with the distribution of MPO by standard immunohistochemistry - MPO was strongly expressed by myeloid blasts and absent in lymphoid blasts; in CML, most, but not all, cells were positive for MPO, in comparison to many fewer positive cells in reactive marrow. Duplex ISH was demonstrated using a probe for bcl-2 together with MPO in 5 bone marrow sections infiltrated by follicular lymphoma (FL). Strong hybridisation signal for bcl-2 was detected in all cells of the paratrabecular aggregates of FL but showed only scattered positivity in the remainder of the bone marrow. Conversely, MPO was absent in the paratrabecular aggregates and present in the myeloid cells in the remainder of the marrow. This pattern was present in both single and duplex ISH for MPO and bcl-2 in the marrow infiltrated by FL. Duplex ISH was performed both by sequential hybridisation with bcl-2 followed by MPO, and simultaneously with a mix of bcl-2 and MPO probes. As negative controls, scrambled oligonucleotide probes for the corresponding genes were used in each case and did not show hybridisation. In summary, we have developed a generic method for QD labelling and semi-quantitative detection of oligonucleotide ISH in routinely processed clinical tissue samples. Although, in this study we primarily used bone marrow trephine samples, this technique can be applied to any tissue. It has the potential to facilitate transfer of microarray-identified gene signatures to clinical research and diagnostics, whilst the ability of spectral imaging to resolve multiple signals offers the possibility of multiplexed probe detection.
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26

Nicholson, J. "Imbalances of chromosome 17 in medulloblastomas determined by comparative genomic hybridisation and fluorescence in situ hybridisation." Molecular Pathology 53, no. 6 (December 1, 2000): 313–19. http://dx.doi.org/10.1136/mp.53.6.313.

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27

Bagnoli, Francesca, Susanna Danti, Valentina Magherini, Radiana Cozza, Anna M. Innocenti, and Milvia L. Racchi. "Molecular cloning, characterisation and expression of two catalase genes from peach." Functional Plant Biology 31, no. 4 (2004): 349. http://dx.doi.org/10.1071/fp03203.

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Two cDNA clones encoding catalase (Cat1 and Cat2) from peach [Prunus persica (L.) Batsch] were identified, that show homologies to other plant catalases. The nucleotide sequences of the two coding regions showed 88% identity to each other. The amino acid sequences predicted from the two full-length clones showed the highest homology to a catalase from cotton and Nicotiana plumbaginifolia L. and included C-terminal tri-peptides typical of those used to target proteins to peroxisomes. Southern hybridisation analysis suggested the existence of two catalase genes in peach. The expression of Cat1 and Cat2 was determined in seeds, vegetative tissue, leaves during the seasonal cycle and in leaves in response to light / dark treatments. Cat1 had high levels of expression only in leaf tissue and was responsive to light and seasonal changes. Cat2 had high levels of expression in in vitro shoots and was also responsive to seasonal changes, but not to light. In situ hybridisations to leaf tissue indicated that the expression of Cat1 was localised mainly in palisade cells, while Cat2 mRNA was present in the vascular tissue. The results of the expression analysis and in situ hybridisation suggest a role for Cat1 in photorespiration and for Cat2 in stress responses.
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28

Fuster, C., L. Miguez, R. Miro, M. A. Rigola, A. Perez, and J. Egozcue. "Familial complex chromosome rearrangement ascertained by in situ hybridisation." Journal of Medical Genetics 34, no. 2 (February 1, 1997): 164–66. http://dx.doi.org/10.1136/jmg.34.2.164.

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29

Gosden, J. R. "In Situ Hybridisation: Application to Developmental Biology and Medicine." Journal of Medical Genetics 28, no. 6 (June 1, 1991): 432. http://dx.doi.org/10.1136/jmg.28.6.432.

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30

Amann, Rudolf, Bernhard M. Fuchs, and Sebastian Behrens. "The identification of microorganisms by fluorescence in situ hybridisation." Current Opinion in Biotechnology 12, no. 3 (June 2001): 231–36. http://dx.doi.org/10.1016/s0958-1669(00)00204-4.

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31

Hopman, A. H. N., Sandra Claessen, and Ernst J. M. Speel. "Multi-colour brightfield in situ hybridisation on tissue sections." Histochemistry and Cell Biology 108, no. 4-5 (October 17, 1997): 291–98. http://dx.doi.org/10.1007/s004180050168.

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32

Troncone, G., S. M. Anderson, C. S. Herrington, M. L. de Angelis, H. Noell, J. A. Chimera, and J. O'D McGee. "Comparative analysis of human papillomavirus detection by dot blot hybridisation and non-isotopic in situ hybridisation." Journal of Clinical Pathology 45, no. 10 (October 1, 1992): 866–70. http://dx.doi.org/10.1136/jcp.45.10.866.

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33

MCFADDEN, G. "In situ hybridisation in plants: From macroscopic to ultrastructural resolution." Cell Biology International Reports 13, no. 1 (January 1989): 3–21. http://dx.doi.org/10.1016/s0309-1651(89)80004-9.

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34

Dodd, Susan, Tahir Naeem Khan, and Raja Sinniah. "An in situ Cytomegalovirus DNA Hybridisation Study in IgA Nephritis." Nephron 59, no. 3 (1991): 527. http://dx.doi.org/10.1159/000186635.

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35

Wilkens, Ludwig, Heidrun Gerr, Dorothea Gadzicki, Hans Kreipe, and Brigitte Schlegelberger. "Standardised fluorescence in situ hybridisation in cytological and histological specimens." Virchows Archiv 447, no. 3 (June 10, 2005): 586–92. http://dx.doi.org/10.1007/s00428-005-1211-9.

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36

Zhao, J. T., M. Frommer, J. A. Sved, and A. Zacharopoulou. "Mitotic and polytene chromosome analyses in the Queensland fruit fly, Bactrocera tryoni (Diptera: Tephritidae)." Genome 41, no. 4 (August 1, 1998): 510–26. http://dx.doi.org/10.1139/g98-053.

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The Queensland fruit fly, Bactrocera tryoni, like the Mediterranean fruit fly, Ceratitis capitata, has a diploid complement of 12 chromosomes, including five pairs of autosomes and a XX/XY sex chromosome pair. Characteristic features of each chromosome are described. Chromosomal homology between B. tryoni and C. capitata has been determined by comparing chromosome banding pattern and in situ hybridisation of cloned genes to polytene chromosomes. Although the evidence indicates that a number of chromosomal inversions have occurred since the separation of the two species, synteny of the chromosomes appears to have been maintained.Key words: tephritid fruit fly, Bactrocera tryoni, polytene chromosomes, in situ hybridisation, chromosomal homology.
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37

Bilous, Michael, Adrienne Morey†, Jane Armes‡, Margaret Cummings, and Glenn Francis. "Chromogenic in situ hybridisation testing for HER2 gene amplification in breast cancer produces highly reproducible results concordant with fluorescence in situ hybridisation and immunohistochemistry." Pathology 38, no. 2 (April 2006): 120–24. http://dx.doi.org/10.1080/00313020600561518.

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38

Lee, Andrew H. S., Heather P. Key, Jane A. Bell, Zsolt Hodi, and Ian O. Ellis. "Breast carcinomas with borderline (2+) HER2 immunohistochemistry: percentage of cells with complete membrane staining for HER2 and the frequency of HER2 amplification: Table 1." Journal of Clinical Pathology 64, no. 6 (March 17, 2011): 490–92. http://dx.doi.org/10.1136/jcp.2011.089177.

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AimHER2 status is vital for selecting breast cancer patients for trastuzumab treatment. One recommended approach is to assess immunohistochemical staining and then perform in situ hybridisation on those tumours with a borderline (2+) immunohistochemical result. This audit aimed to assess the value of the percentage of immunohistochemical staining in 2+ tumours in selecting tumours for in-situ hybridisation.MethodsHER2 immunohistochemistry and in situ hybridisation was performed according to UK guidelines. The percentage of complete membrane staining of invasive carcinoma cells for HER2 was recorded as part of routine reporting.Results191 (11%) of 1735 invasive carcinomas were scored as 3+. 419 (24%) were scored as 2+. 57 of 413 2+ carcinomas (14%) were amplified (ratio of HER2 to chromosome 17≥2.0). The frequency of amplification was related to the percentage of complete membrane staining: eight of 149 (5%) with 10–19% membrane staining, 11 of 93 (12%) with 20–29% staining, 26 of 150 (17%) with 30–79% staining and 12 of 21 (57%) with 80–100% staining.ConclusionsThis audit suggests that increasing the threshold for 2+ from 10% to 20% complete membrane staining would reduce the number of in-situ hybridisation tests by 36%, but reduce the detection of amplified tumours by 14%.
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39

Kuipers, Anja GJ, Pat JS Heslop-Harrison, and Evert Jacobsen. "Characterisation and physical localisation of Ty1-copia-like retrotransposons in four Alstroemeria species." Genome 41, no. 3 (June 1, 1998): 357–67. http://dx.doi.org/10.1139/g98-048.

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The genus Alstroemeria contains species with large genomes (2C = 36.5-78.9 pg (17 600 - 38 000 Mb) in those species with 2n = 2x = 16). We investigated the diversity and genomic and chromosomal organisation of Ty1-copia-like retrotransposons in four Alstroemeria species. Analysis of 33 PCR-amplified sequences corresponding to a conserved domain of the Ty1-copia reverse transcriptase (rt) gene showed high heterogeneity among predicted amino acid sequences; no two sequences were identical, but most fell into one of five subgroups. Levels of inter- and intra-specific heterogeneity of sequences were similar. HaeIII-digested genomic DNA of various Alstroemeria species contained distinct bands upon hybridisation with individual rt gene fragments. Hybridisation with the heterogeneous PCR pool of rt fragments (retrotransposon pool) revealed additional bands; some minor bands were characteristic of either Brazilian or Chilean species. In situ hybridisation of the retrotransposon pool from three species to metaphase chromosomes from the same species showed a dispersed distribution of the retrotransposon pool with exclusion from rDNA and other chromosomal sites.Alstroemeria pelegrina, which is without major heterochromatic sites, showed some clustering and small negative bands. The retrotransposon pool was excluded from major DAPI-staining bands in Alstroemeria aurea, but in contrast, the sites of the major tandemly repeated sequences in Alstroemeria inodora showed a hybridisation signal similar to that in the rest of the chromosomes. The data are discussed in the context of the contribution of Ty1-copia-like retrotransposons to plant genome size, their evolution, and their value for phylogenetic and biodiversity studies.Key words: Alstroemeria, in situ hybridisation, genome organisation, retrotransposable elements, Ty1-copia.
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40

Stonati, L., M. Durante, G. Gensabella, G. Gialanella, G. F. Grossi, M. Pugliese, P. Scampoli, A. Sgura, A. Testa, and C. Tanzarella. "Calibration Curves for Biological Dosimetry by Fluorescence In situ Hybridisation." Radiation Protection Dosimetry 94, no. 4 (April 2, 2001): 335–45. http://dx.doi.org/10.1093/oxfordjournals.rpd.a006508.

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41

Lory, S., C. von Tscharner, E. Marti, G. Bestetti, S. Grimm, and A. Waldvogel. "In situ hybridisation of equine sarcoids with bovine papilloma virus." Veterinary Record 132, no. 6 (February 6, 1993): 132–33. http://dx.doi.org/10.1136/vr.132.6.132.

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42

Ramage, Gordon, Sheila Patrick, and Simon Houston. "Combined fluorescent in situ hybridisation and immunolabelling of Bacteroides fragilis." Journal of Immunological Methods 212, no. 2 (March 1998): 139–47. http://dx.doi.org/10.1016/s0022-1759(98)00005-2.

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43

Warford, Anthony. "In situ hybridisation: Technologies and their application to understanding disease." Progress in Histochemistry and Cytochemistry 50, no. 4 (January 2016): 37–48. http://dx.doi.org/10.1016/j.proghi.2015.12.001.

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44

Conte, R. A., S. Luke, and R. S. Verma. "Enumeration of semen leucocytes by fluorescence in situ hybridisation technique." Molecular Pathology 48, no. 6 (December 1, 1995): M319—M321. http://dx.doi.org/10.1136/mp.48.6.m319.

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45

Osborne, Peter, and Peter K. Dearden. "Non-radioactive in-situ hybridisation to honeybee embryos and ovaries." Apidologie 36, no. 1 (January 2005): 113–18. http://dx.doi.org/10.1051/apido:2004075.

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46

Chatzimeletiou, Katerina, George Makrydimas, Alexandros Sotiriadis, Evagelos Paraskevaidis, and Kypros H. Nicolaides. "Aneuploidy screening in coelomic samples using fluorescencein situ hybridisation (FISH)." Prenatal Diagnosis 25, no. 10 (2005): 919–26. http://dx.doi.org/10.1002/pd.1227.

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47

Brenk, Christian H., Eva-Christina Prott, Detlef Trost, Alexander Hoischen, Constanze Walldorf, Bernhard Radlwimmer, Dagmar Wieczorek, et al. "Towards mapping phenotypical traits in 18p− syndrome by array-based comparative genomic hybridisation and fluorescent in situ hybridisation." European Journal of Human Genetics 15, no. 1 (October 4, 2006): 35–44. http://dx.doi.org/10.1038/sj.ejhg.5201718.

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48

Kokubugata, G., K. Kondo, G. W. Wilson, L. M. Randall, A. van der Schans, and D. K. Morris. "Comparison of karyotype and rDNA-distribution in somatic chromosomes of Bowenia species (Stangeriaceae, Cycadales)." Australian Systematic Botany 13, no. 1 (2000): 15. http://dx.doi.org/10.1071/sb98028.

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Somatic chromosomes at mitotic metaphase of Bowenia serrulata, B. spectabilis and B. sp. ‘Tinaroo’ is investigated by the standard aceto-orcein staining method and the fluorescent in situ hybridisation method (FISH) with ribosomal DNA (rDNA) probe. Bowenia serrulata, B. spectabilis and B. sp. ‘Tinaroo’ each have a chromosome number of 2n = 18. The karyotype of B. serrulata exhibits 10 median-centromeric chromosomes, while B. spectabilis and B. sp. ‘Tinaroo’ exhibit eight median-centromeric chromosomes. By using FISH, B. serrulata, B. spectabilis and B. sp. ‘Tinaroo’ show a hybridisation signal on the satellite of the short arm of two submedian-centromeric chromosomes. However, the other hybridisation signal pattern is different among B. serrulata, B. spectabilis and B. sp. ‘Tinaroo’.
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49

Langer, Rupert, Sandra Rauser, Marcus Feith, Jörg M. Nährig, Annette Feuchtinger, Helmut Friess, Heinz Höfler, and Axel Walch. "Assessment of ErbB2 (Her2) in oesophageal adenocarcinomas: summary of a revised immunohistochemical evaluation system, bright field double in situ hybridisation and fluorescence in situ hybridisation." Modern Pathology 24, no. 7 (April 22, 2011): 908–16. http://dx.doi.org/10.1038/modpathol.2011.52.

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50

Schrire, Timothy, Benjamin Patel, and Shelley Potter. "P021. In-house in-situ hybridisation accelerates indeterminate HER2 lesion results." European Journal of Surgical Oncology 47, no. 5 (May 2021): e301-e302. http://dx.doi.org/10.1016/j.ejso.2021.03.025.

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