Dissertations / Theses on the topic 'In-situ hybridisation'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 49 dissertations / theses for your research on the topic 'In-situ hybridisation.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.
Princivalle, Alessandra Patrizia. "Studies of GABAb receptors in epilepsy." Thesis, University of Birmingham, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.289300.
Full textHoyle, Jane Anthea. "In situ hybridisation for the detection of viral nucleic acids." Thesis, University of St Andrews, 1991. http://hdl.handle.net/10023/13924.
Full textHauxwell, Angela Jane. "In situ hybridisation for studying embryo development in Pisum sativum L." Thesis, University of East Anglia, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.279676.
Full textHajMohammadi, Sassan. "Development of FISH technology in pathological tissue." Thesis, University of Southampton, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.284578.
Full textWu, Yih-Yiing. "Response of skin to noxious stimuli : studies using in situ hybridisation." Thesis, University of Newcastle Upon Tyne, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.263124.
Full textBirchall, Philip Simon. "Multicolour fluorescence in situ hybridisation to RNA in whole-mount Caenorhabditis elegans." Thesis, University of Cambridge, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.296668.
Full textZheng, Yun-Ling. "Rapid prenatal diagnosis of common fetal aneuploidies by fluorescence in situ hybridisation." Thesis, University of Cambridge, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.318418.
Full textTeo, Chong Gee. "Analysis of the Epstein-Barr virus-host relationship by in situ hybridisation." Thesis, Imperial College London, 1989. http://hdl.handle.net/10044/1/47684.
Full textMohaddes, Ardebili Seyed Mojtaba. "Optimisation of interphase fluorescence in situ hybridisation for detection of common aneuploidies." Thesis, Connect to e-thesis, 1996. http://theses.gla.ac.uk/692/.
Full textPh.D. thesis submitted to the Faculty of Medicine, Department of Division of Developmental Medicine, University of Glasgow, 1996. Includes bibliographical references: p. 118-132. Print version also available.
Hammond, David William. "Analysis of non-Hodgkin's lymphoma by conventional cytogenetics and fluorescence in-situ hybridisation." Thesis, University of Sheffield, 1995. http://etheses.whiterose.ac.uk/3064/.
Full textJaffe, Benjamin. "Genome analysis of Hordeum bulbosum L. and hybrids with H. vulgare L." Thesis, University of Reading, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302327.
Full textTaylor, Clare Petronella Florence. "The use of fluorescence in situ hybridisation techniques in the diagnosis and prognosis of malignancy." Thesis, University College London (University of London), 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.297282.
Full textBuckle, V. J. "The application of in situ hybridisation to the genetic analysis of the X chromosome." Thesis, University of Oxford, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.370315.
Full textSinclair, Paul Burdwood. "A fluorescence in-situ hybridisation and molecular investigation of leukaemia associated abnormalities of chromosome 6q." Thesis, University College London (University of London), 2002. http://discovery.ucl.ac.uk/1382604/.
Full textWilliams, Lisa. "Fluorescence in situ hybridisation (FISH) analysis of chromosomal aberrations in gastric tissue : the involvement of Helicobacter pylori." Thesis, Swansea University, 2004. https://cronfa.swan.ac.uk/Record/cronfa43067.
Full textShone, John William. "Development and implementation of fluorescent in situ hybridisation for the detection of human cutaneous bacterial microflora." Thesis, Institute of Cancer Research (University Of London), 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.511179.
Full textTurner, Gabrielle M. "Development of in situ hybridisation to examine tissue-specific expression patterns of the invertase genes in sugarcane culm." Thesis, Stellenbosch : University of Stellenbosch, 2005. http://hdl.handle.net/10019.1/16621.
Full textENGLISH ABSTRACT: The goals of this project were firstly to develop the tissue preparation and in situ hybridisation protocols for sugarcane culm tissue, and secondly to use the developed techniques to examine the expression patterns of three invertase isoforms in sugarcane internodes of various developmental stages. Sugarcane invertases have been the focus of intense research for many years, yet almost nothing is known of their tissue-specific distribution. It was thought that by characterising their expression patterns using in situ hybridisation, more knowledge of their functions and involvement in sucrose accumulation would be gained. Although in situ hybridisation is now regularly used to study gene expression in plants, there is to date only a single publication describing its use on immature sugarcane tissue. Therefore this technique needed further development, and this was achieved by comparing different tissue preparation methods, as well as by systematically testing the various parameters pertaining to each method. The in situ hybridization technique was also developed by testing and comparing a number of key parameters. It was found that fixing whole mount tissue for 48 h preserved sugarcane tissue adequately. High hybridization temperatures and probe concentrations provided the best signal, and including pre-treatment with HCl and Pronase was essential in sensitizing the tissue to the probe. A less viscous detection buffer reduced both osmotic effects and time required for signal detection. In the second part of this study, the developed method was used to examine the expression patterns of the three invertase isoforms in young, maturing and mature internodes of sugarcane, and the results were complemented with Northern blot analysis. Transcript of all three isoforms was found to be present in the storage parenchyma and in the phloem tissue. Transcript levels of all three isoforms declined in maturing tissue, with soluble acid invertase declining sharply and dropping below detection in maturing and mature tissue. Transcript levels of cell wall invertase and neutral invertase declined only gradually, and appreciable levels of both were still present in mature tissue. Acid invertase is suggested to be mainly involved in internode elongation, while cell wall invertase would appear to play important roles in phloem unloading and turgor control. Neutral invertase is suggested to be involved in either sucrose cycling or maintenance of hexose pools, however the function of this enzyme remains unclear. This study has demonstrated the value of in situ hybridization, yet at the same time has shown its limitations, especially when more traditional biochemical techniques are not employed to complement the results. Although the precise functions of the invertase isoforms in sugarcane remain inconclusive, this study has opened up the way for tissuespecific promoter design and future in situ studies of sugarcane invertases
AFRIKAANSE OPSOMMING: Die doel van hierdie projek was tweeledig: eerstens om weefselvoorbereiding en in situhibridisasie- protokolle vir die stingelweefsel van suikerriet te ontwikkel; en tweedens om die ontwikkelde tegnieke te gebruik om die uitdrukkingspatrone van drie invertaseisovorme in die suikerriet-internodes van verskeie ontwikkelingstadia te ondersoek. Suikerriet-invertases is al vir jare lank die fokus van intense navorsing, maar baie min is bekend oor hulle weefselspesifieke verspreiding. Die idee was om meer kennis oor suikerriet-invertases se funksies en betrokkenheid by sukrose-akkumulasie te verkry deur in situ-hibridisasie te gebruik om hulle uitdrukkingspatrone te karakteriseer. Alhoewel in situ-hibridisasie deesdae gereeld gebruik word om geenuitdrukking in plante te bestudeer, is daar tot op datum slegs een publikasie wat die gebruik daarvan in onvolwasse suikerrietweefsel beskryf. Hierdie tegniek moes dus verder ontwikkel word, en dit is gedoen deur verskillende weefselvoorbereidingsmetodes te vergelyk en sistematies die verskillende parameters wat op elke metode van toepassing is te toets. Die in situ-hibridisasie-tegniek is ook ontwikkel deur die toetsing en vergelyking van 'n aantal sleutelparameters. Daar is gevind dat suikerrietweefsel voldoende gepreserveer word deur die intakte gemonteerde weefsel vir 48 uur te fikseer. Hoë hibridisasietemperature en hoë peilerkonsentrasies het die beste sein gegee; die insluiting van voorbehandeling met HCl en Pronase was noodsaaklik om die weefsel meer gevoelig vir die peiler te maak. Osmotiese invloede en die tyd nodig vir seindeteksie is verminder deur die viskositeit van die buffer te verminder. In die tweede deel van die studie is die ontwikkelde metode gebruik om die uitdrukkingspatrone van die drie invertase-isovorme in jong, ontwikkelende en volwasse internodes te ondersoek en die resultate is deur 'n noordelike oordraganalise gekomplementeer. Transkripte van al drie isovorme is in die stoorparenchiem en floëemweefsel gevind. Transkripvlakke van al drie isovorme het afgeneem in ontwikkelende weefsel, met oplosbare suurinvertase wat skerp afgeneem en tot onder die deteksie-limiet gedaal het in ontwikkelende en volwasse weefsel. Transkripvlakke van selwandinvertase en neutrale invertase het slegs geleidelik afgeneem en merkbare vlakke van albei was teenwoording in ontwikkelende en volwasse weefsel. Daar word voorgestel dat suurinvertase hoofsaaklik betrokke is by internodeverlenging, terwyl selwandinvertase skynbaar 'n belangrike rol in floëem-ontlading en turgor-beheer speel. Daar word voorgestel dat neutrale invertase betrokke is óf by die sukrose-sirkulering óf by die onderhoud van heksose-poele; die funksie van hierdie ensiem is egter steeds nie duidelik nie. Hierdie studie het die waarde van in situ-hibridisasie gedemonstreer maar terselfdetyd ook die beperkinge daarvan uitgewys, veral as meer tradisionele biochemiese tegnieke nie gebruik word om die resultate aan te vul nie. Alhoewel daar onsekerheid is oor die presiese funksies van die invertase-isovorme in suikerriet, het die studie die weg gebaan vir weefselspesifieke promotorontwerp en toekomstige in situ-studies van suikerrietinvertases.
Wall, Wilson. "A study of variation in human chromosomes shown by photometry, G, C, replication banding and in situ hybridisation." Thesis, Open University, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.314792.
Full textStindl, Martin Maria Matthias. "Evaluation of ligation methods and the synthesis of a specific PNA-encoded peptide library." Thesis, University of Edinburgh, 2015. http://hdl.handle.net/1842/17927.
Full textVirgo, Lisa. "A study of neurotransmitter function in motor neurone disease : using the techniques of in situ hybridisation and receptor autoradiography." Thesis, Imperial College London, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.336581.
Full textAchuthan, Rajgopal. "An evaluation of the chromosome 1p region and the BCL10 gene in malignant lymphoma, by fluorescent in-situ hybridisation." Thesis, University of Leeds, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.414499.
Full textRamoutar, Rakeshnie. "The development of an in situ hybridisation technique to determine the gene expression patterns of UDP-Glucose dehydrogenase, pyrophosphate-dependent phosphofructokinase and UDP-Glucose pyrophosphorylase in sugarcane internodal tissues." Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/49795.
Full textENGLISH ABSTRACT: The cellular expression of the enzymes implicated in regulating sucrose metabolism and accumulation in sugarcane is poorly understood. The present study was therefore aimed at the development of an in situ hybridisation (ISH) technique to study differential gene expression among the various cell types of the sugarcane culm. This technique in conjunction with northern and western blotting was then used to determine the sites of cellular and tissue specific expression of the cytosolic enzymes, UDP-Glc dehydrogenase, pyrophosphate dependent phosphofructokinase and UDP-Glc pyrophosphorylase, involved in sucrose metabolism. This study revealed that the determination of the influencing parameters associated with the development of an ISH protocol was essential for the successful detection of the endogenous RNA sequences in sugarcane internodal tissues. The parameters that were investigated included the type of embedding medium, duration of fixation period, pre-treatment procedures and hybridisation temperature. It further revealed that fresh internodal tissue sections, fixed for a period of 24 h and thereafter exposed to pre-treatment and hybridisation, facilitated the analysis of cytological gene expression at all stages of sugarcane development. The second part of this study revealed very localised transcript expression for UDP-Glc DH, PFP and UGPase in the different internodal tissue and cell types. The UDP-Glc DH and UGPase transcripts were localised to the phloem elements, whilst xylem tissue only expressed the UDP-Glc DH transcript. Transcripts of UDP-Glc DH, PFP and UGPase were all expressed in the parenchyma cells that were associated with the vascular bundles and the stem storage compartment, suggesting that the parenchyma cells distributed throughout the stem in the different tissue types complement each other in function for the purposes of phloem loading, unloading and assimilate transport processes. Complimentary northern and western hybridisations demonstrated that internode 7 represents a shift in the sink from utilisation to storage. This is evident by the observed decline in both the relative transcript and protein abundances of UDP-Glc DH, PFP and UGPase at this stage of development. The relative mRNA and protein abundances for the three enzymes showed a similar trend. Higher levels of the gene transcripts and translated products were observed in the younger sucrose importing tissues, than in the older sucrose accumulating internodes. At a cellular level, it was found that the sites of cellular UDP-Glc DH, PFP and UGPase expression differed marginally. Whilst UDP-Glc DH was expressed in the phloem, xylem and parenchyma cells of the vascular complex and in storage parenchyma cells, PFP was expressed exclusively in parenchyma cells that were associated with the vascular bundles and those serving a storage function in the stem pith and UGPase was found to be localised in the phloem and parenchyma of the vascular bundles and the storage parenchyma cells. Such findings have demonstrated an increase in resolution with which gene expression can be examined at a cellular level. Hence, the results from this study have demonstrated that the knowledge of metabolic compartmentation between different tissue and cell types is a requisite to understanding the function(s) of individual enzymes within complex structures such as the sugarcane culm.
AFRIKAANSE OPSOMMING: Die sellulêre lokalisering van die ensieme wat geïmpliseer word in die regulering van sukrose metabolisme is onbekend. Met dit in gedagte, was hierdie studie gefokus op die ontwikkeling van 'n in situ hibridisasie (ISH) tegniek om differensiële geenuitdrukking in die verskillende seltipes van die suikerrietstingel te ondersoek. Hierdie tegniek, tesame met RNA-en proteïen gel blots, is volgens aangewend om die areas van sellulêre-en weefselspesifieke uitdrukking van die sitosoliese ensieme UDP-glukose dehydrogenase, pirofosfaat-afhanklike fosfofruktokinase en UDP-glukose pirofosforilase, wat almal betrokke is by sukrosemetabolisme, te bepaal. Dit het duidelik geword gedurende die studie dat die bepaling van die optimale parameters van die ISH protokol vir suikerriet van deurslaggewende belang sou wees vir die opsporing van endogene RNA volgordes. Die parameters wat ondersoek is het ingesluit die tipe inbeddingsmedium, die tydsduur van fiksering, vooratbehandelings- en hibridisasiemetodes. Dit het duidelik geword dat vars internodale weefselsnitte wat vir 24 h gefikseer is en daarna voorafbehandeling en hibridisasie ondergaan het, die bepaling van geenuitdrukking tydens alle fases van suikkerrietontwikkeling moontlik gemaak het. Die tweede fase van hierdie studie het aangetoon dat al drie ensieme spesifiek gelokaliseerde uitdrukkingspatrone gehad het in verskillende internodale weefsels en seltipes. Al drie gene is konstitutief uitgedruk in internodes. Die UDP-glukose dehydrogenase en UDP-glukose pirofosforilase transkripte is gelokaliseer na die floeëm elemente, terwyl xileem slegs die UDP-glukose dehydrogenase transkripte bevat het. Al die gene is in die parenchiemselle uitgedruk wat geassosieer is met die vaatbondels en die stingel stoorkompartement, wat moontlik beteken dat die parenchiem selle wat deur die stingel versprei is 'n sentrale netwerk vorm wat direk of indirek koolstofassimileringsprosesse beïnvloed. RNA-en proteïen gel blots op dieselfde internodes het gewys dat internode sewe 'n verskuiwing, van koolstofverbruik na berging, verteenwoordig. Dit word gerllustreer deur die afname in beide transkrip en proteïen vlakke van die drie ensiem in hierdie stadium van ontwikkeling. Alhoewel beide mRNA en proteïen vlakke vir al die ensieme 'n soortgelyke tendens getoon het, het die sellulêre uitdrukking van die ensieme volgens ISH verskil, wat die krag van die tegniek illustreer. Die resultate van hierdie studie het gedemonstreer dat begrip van die kompartementalisasie van metabolisme tussen verskillende weefsel-en seltipes 'n voorvereiste is om die funksie/s van individuele ensieme in komplekse strukture soos die suikerrietstingel te bepaal.
Pedras, Maria Inês Machado. "Investigation of the regulation mechanisms for bioplastics production from industrial residues." Master's thesis, Faculdade de Ciências e Tecnologia, 2013. http://hdl.handle.net/10362/10863.
Full textThe current high demand for plastics has become unsustainable. Polyhydroxyalkanoates are biopolymers stored by bacteria that can potentially replace modern plastics due to: wide range of applications; biodegradability; use of renewable resources as feedstock. High costs of current Polyhydroxyalkanoates production can be reduced using mixed cultures of organisms. Activated sludge from wastewater treatment plants is selected for Polyhydroxyalkanoates production through the imposition of cycles of intermittent feeding. In this study, the acclimation of activated sludge using synthetic volatile fatty acids (VFAs) as substrate resulted in a culture rich in Paracoccus spp. and unidentified filamentous bacteria. Low cost substrates such as sugarcane molasses (SM) or cheese whey (CW) can be employed as feedstock for further cost reduction. This requires an additional step before the microbial selection to ferment the feedstock into VFAs. In this work, the feedstock was changed from SM to CW. The population fed with SM was rich in Actinomycetaceae, while the population fed with CW was rich in Streptococcaceae, affecting the VFA composition. Consequently, the PHA-storing population and the polymer were affected. In the fermented SM (fSM) phase, the population was rich in Azoarcus (41.5 - 64.6%) and in the fCW phase the population was more diverse. Changing the pH in the fermentation reactor also affected the selection stage with an increase in Thauera and Azoarcus and a decrease in Paracoccus. A significant unidentified population of one layer sheet- forming bacteria was observed. Lastly, the occurrence of cell-to-cell communication (QS) in the selection stage was investigated. Possibly, QS molecules were detected when the carbon source was depleted. All steps of polyhydroxyalkanoate production are interconnected and for optimization, all stages must be studied and improved. Moreover, if QS proves to be involved in polyhydroxyalkanoate storage, the addition of QS molecules to the process may be explored for further optimization.
com, dbearham@hotmail, and Douglas Bearham. "Identification and characterisation of two haplosporidian parasites of oysters in north Western Australia." Murdoch University, 2008. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20081114.120135.
Full textBardin, Philip Greyling. "Human rhinovirus : development of an experimental disease program and detection in tissue employing in situ hybridisation and a polymerase chain reaction." Thesis, University of Southampton, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.296349.
Full textTholouli, Eleni. "The development of a novel quantum dot labelled oligonucleotide in situ hybridisation technique for analysis of gene expression in acute myeloid leukaemia." Thesis, University of Manchester, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.527409.
Full textWilkins, Bridget Sally. "Cell-stroma interactions in haemopoiesis studied by immunocytochemistry and in situ hybridisation in long-term cultures and trephine biopsies of human bone marrow." Thesis, University of Southampton, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.242221.
Full textLeversha, Margaret Anne. "Cytological estimations of molecular genetic difference : applications and implications of fluorescence in situ hybridisation mapping in the long arm of human chromosome 9." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.337902.
Full textVontell, Regina Theresa. "Expression of toll-like receptor 3 in the preterm brain after white matter injury : a post-mortem study applying immunohistochemistry and in situ hybridisation." Thesis, King's College London (University of London), 2015. https://kclpure.kcl.ac.uk/portal/en/theses/expression-of-tolllike-receptor-3-in-the-preterm-brain-after-white-matter-injury(0eaf3659-2e87-46cb-bc4d-edff67b81e4d).html.
Full textSomasekar, Amudha. "Fluorescence in situ hybridisation (FISH) analysis of chromosome 1 and gene expression levels of MAD2 and BUB1 levels in premalignant stages of gastric tissue." Thesis, Swansea University, 2011. https://cronfa.swan.ac.uk/Record/cronfa43141.
Full textPäällysaho, S. (Seliina). "Contribution of X chromosomal and autosomal genes to species differences in male courtship songs of the Drosophila virilis group species." Doctoral thesis, University of Oulu, 2001. http://urn.fi/urn:isbn:9514265831.
Full textCaburet, Sandrine. "Structure mosai͏̈que et instabilité de l'ADN ribosomal humain : implications dans la sénescence et la cancérogenèse." Paris 7, 2002. http://www.theses.fr/2002PA077038.
Full textVarela, Filipa Alexandra Pereira Rosa. "Tipificação de Mycoplasma mycoides subsp. mycoides SC e detecção da sua aderência a células epiteliais pulmonares." Master's thesis, Faculdade de Ciências e Tecnologia, 2010. http://hdl.handle.net/10362/5092.
Full textA Peripneumonia Contagiosa Bovina (PPCB) é uma doença respiratória infecciosa, de grande relevo no âmbito da produção de gado bovino, causada pela bactéria Mycoplasma mycoides subsp. mycoides SC (MmmSC). Actualmente, a PPCB permanece endémica apenas em África, sendo aí responsável por perdas incontornáveis no sector pecuário. O último surto de PPCB detectado na Europa ocorreu em Portugal em 1999 mas, devido à sua natureza insidiosa, o risco de re-emergência é permanente. As estirpes de MmmSC associadas aos últimos surtos europeus de PPCB são tradicionalmente consideradas geneticamente muito homogéneas. No entanto, trabalhos recentes de tipificação molecular revelaram a existência de uma variabilidade genética intraspecífica superior ao que se esperava. A realização deste trabalho teve como base dois objectivos fundamentais: a tipificação de estirpes de MmmSC isoladas durante os últimos surtos de PPCB que ocorreram em Portugal entre 1993 e 1998, e a aplicação de um método alternativo e inovador para a detecção e quantificação de MmmSC, após a infecção de culturas celulares. Para a concretização do primeiro objectivo foi realizada uma análise da variabilidade polimórfica de três regiões VNTR (Variable Number of Tandem Repeats) existentes no genoma de estirpes de MmmSC. O locus VNTR4 comprovou ser o mais polimórfico, detectando-se quatro perfis distintos entre as estirpes portuguesas. O perfil VNTR4 do tipo “9” (numerado em função das repetições da sequência de consenso) revelou-se amplamente distribuído geograficamente e foi predominante entre os isolados. No entanto, observou-se uma segregação geográfica de perfis, dado que na região da Beira Litoral apenas foram encontradas estirpes com o perfil VNTR4 do tipo “8”. Estes resultados sugerem que podem ter ocorrido, pelo menos, dois eventos de re-emergência de PPCB em Portugal, entre 1993 e 1998. Para a realização do segundo objectivo deste trabalho foi optimizada uma técnica baseada na hibridação in situ com sondas de DNA fluorescentes (FISH) que permitiu visualizar micoplasmas aderidos a culturas celulares. Esta técnica, que nunca tinha sido usada previamente na detecção de MmmSC, comprovou ser adequada para futuras aplicações em estudos de citoaderência e para a detecção de micoplasmas em culturas celulares.
Karlsson, Christina. "Biomarkers in non-small cell lung carcinoma : methodological aspects and influence of gender, histology and smoking habits on estrogen receptor and epidermal growth factor family receptor signalling." Doctoral thesis, Örebro universitet, Hälsoakademin, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-19725.
Full textKrämer, Dorothee Charlotte Agathe. "Investigation of Mammalian Chromatin Folding at Different Genomic Length Scales using High Resolution Imaging." Doctoral thesis, Humboldt-Universität zu Berlin, 2019. http://dx.doi.org/10.18452/19929.
Full textChromatin needs to organize gene regulation whilst fitting into the confined space of the nucleus. Chromatin organization is therefore intertwined with gene activation and silencing. In recent years many advances in the field of chromatin architecture have been made showing that chromatin is organized hierarchically. Folding occurs in subsequent units, where each level of organization contributes to the spatial compaction of DNA and gene regulation. In this dissertation different levels of 3D chromatin organization were analysed using single-cell, high-resolution imaging. On the smallest scale, the 3D organization of two neighbouring Topologically Associating Domains (TADs) at the Sox9 locus was investigated. Performing Fluorescence in situ Hybridization (FISH) in 3D and cryosectioned mouse embryonic stem cells, extensive contacts between the two neighbouring TADs across the TAD boundary were detected. Applying FISH in a cell line bearing a genomic duplication within the Sox9 locus, the occurrence of two different conformations that result from the duplication was shown. Recent evidence from GAM showed the formation of long-range, multimer contacts between distal regulatory elements. Investigating the occurrence of long-range contacts between super-enhancer TADs in single cells by FISH, showed that they establish frequent interactions at close spatial distances. Furthermore the formation of clusters containing distal super-enhancer TADs could be demonstrated, indicating the possibility of higher-order regulatory hubs between these enhancer-rich regions. Further investigation showed that super-enhancer regions form different clusters in different cell types. Finally, it was shown that super-enhancers are highly decondensed and preferentially located at splicing speckles.
Andersson, Ann-Catrin. "Studies on Human Endogenous Retroviruses (HERVs) with Special Focus on ERV3." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2002. http://publications.uu.se/theses/91-554-5342-2/.
Full textBetts, Jill Frances. "D-amino acid oxidase, D-serine and the dopamine system : their interactions and implications for schizophrenia." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:de02286f-3e33-4e3e-bc5b-222b62a28ba5.
Full textPetry, Frauke. "Charakterisierung eines neuen ATP-binding-cassette-Transporters aus der ABCA-Subfamilie." Doctoral thesis, [S.l.] : [s.n.], 2004. http://webdoc.sub.gwdg.de/diss/2004/petry/petry.pdf.
Full textDavey, John William. "Identification of b-catenin and other RNAs in developing thalamic axons." Thesis, University of Edinburgh, 2009. http://hdl.handle.net/1842/4011.
Full textXu, Meng. "Specialised transcription factories." Thesis, University of Oxford, 2008. http://ora.ox.ac.uk/objects/uuid:a41d3243-c233-491a-916b-4e329cace434.
Full textBui, Loan Thuy. "Localisation of kallikreins in the prostate and association with prostate cancer progression." Thesis, Queensland University of Technology, 2006. https://eprints.qut.edu.au/16276/1/Loan_Bui_Thesis.pdf.
Full textBui, Loan Thuy. "Localisation of kallikreins in the prostate and association with prostate cancer progression." Queensland University of Technology, 2006. http://eprints.qut.edu.au/16276/.
Full textHenwood, Anthony F. "The demonstration of estrogen receptors in various tumours a study using immunohistochemistry and in situ hybridisation /." 2004. http://hdl.handle.net/2440/37705.
Full textThesis (M.Sc.)--Department of Anatomical Sciences, 2004.
Friedman, Brett. "Characterisation of a chromosomal translocation in an ovarian carcinoma cell line using fluorescence 'in situ' hybridisation." Thesis, 1996. http://hdl.handle.net/10539/22288.
Full textThe region Ilpl3-pl5 on the short arm of chromosome 11 (lip) has been implicated in the initiation or progression of several human malignancies including the embryonic rhabdomyosarcoma Wilms' tumour, bladder, renal cell and ovarian carcinoma. In this study, Fluorescence In Situ hybridisation (FISH) was used to identify the nature of a chromosome llp+ abnormality present in two ovarian carcinoma cell lines after conventional cytogenetic techniques had failed to elucidate the chromosomal origin of the abnormality. Using whole chromosome library probes, the abnormality in cell line UW0V2 was found to be composed entirely of chromosome 3 material representing the translocation t (3;11) (pl2-14;pl5). In the protein-free subline UW0V2(Sf), the abnormality was found to consist of the complex translocation t (3;8; 11) (pl2-14 ;q22-24;pl5) . It is possible that the involvement of chromosome 8 in this translocation was a cell culture phenomenon. Other structural and numerical abnormalities elucidated with FISH in cell line UW0V2(Sf) included lq+, +5, +7, 7q-, 8q+, +12, +14, 14q+, -15, 16q- and -18. Using FISH together with the gene probe pSB|5 and the CEPH YAC probes 892g9, 785e5, 847al2, 954f4, 966e8 and 845a3, the breakpoint region on chromosome 11 in the _ two cell lines was narrowed down and mapped to the region Ilpl4.3-pl5.1 lying between probes 966e8 (D11S902) and 845a3 (D11S899). This represents a physical distance of approximately 1 Mb. The breakpoint in the two cell lines appeared to involve the same region on llplS.l. In a separate study, three epithelial ovarian tumour specimens and four ascitic fluid specimens were obtained. Tumour specimens T2 and T4 and ascitic fluid specimens AF-1, AF-2 and AF-3 were all cytogenetically uninformative. Cytogenetic analysis of specimen T5 revealed a single clonal abnormality involving a deletion in the region 6q21. Ascitic fluid specimen AF-5 yielded cytogenetically normal metaphases. Both specimens were hypodiploid and revealed a cytogenetically normal chromosome 11. Using FISH and CEPH YAC probes 966e8 and 845a3, no abnormalities were detected in the region llpl4.3-pl5.1 in these two specimens but one cannot rule out the possibility of submicroscopic abnormalities lying within the region between these probes. From this study we speculate that chromosome 6 abnormalities may be important in the initiation of these tumours. From the results obtained with cell lines UW0V2 and UW0V2 (Sf) we speculate that the chromosome 3 abnormalities were an early event in the evolution of these tumours while the chromosome 11 abnormality was a later event. Little is known about the region llpl4.3-pl5.1 and very few disease loci have been assigned to this region, however, we may speculate that this region harbours a tumour suppressor gene or an oncogene whose disruption or activation is critical to the pathophysiology of ovarian carcinoma and other genitourinary cancers.
WHSLYP2017
Swaneburg, Uwe [Verfasser]. "Detektion von Porphyromonas gingivalis mittels Fluoreszenz-in-Situ-Hybridisation (FISH-Technik) / vorgelegt von Swaneburg, Uwe, geb. Patzer." 2006. http://d-nb.info/992236258/34.
Full textPeterková, Kristýna. "Lokalizace a kvantifikace mRNA kódující trávící peptidázy motolice Fascioloides magna." Master's thesis, 2019. http://www.nusl.cz/ntk/nusl-397285.
Full textWedi, Edris. "Untersuchung des CFL-Phänotyps ("congenital fused labia" ) in dem Neuweltaffen Common Marmoset (Callithrix jacchus) unter demographischen, physiologischen und zytogenetischen Gesichtspunkten." Doctoral thesis, 2010. http://hdl.handle.net/11858/00-1735-0000-0006-AFBB-D.
Full textGibson, Catherine Elizabeth. "The expression of hydrolytic enzymes in germinating barley grain." Thesis, 2017. http://hdl.handle.net/2440/114266.
Full textThesis (Ph.D.) -- University of Adelaide, School of Agriculture, Food and Wine, 2018
Kotz, Matěj. "Karyotypová evoluce u vybraných čeledí entelegynních pavouků." Master's thesis, 2020. http://www.nusl.cz/ntk/nusl-435838.
Full text