Relevant bibliographies by topics / In-situ hybridisation

Academic literature on the topic 'In-situ hybridisation'

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Published: 4 June 2021
Last updated: 1 February 2023

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Journal articles on the topic "In-situ hybridisation"

1

Herrington, C. S. "Demystified ... in situ hybridisation." Molecular Pathology 51, no. 1 (February 1, 1998): 8–13. http://dx.doi.org/10.1136/mp.51.1.8.

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Cassidy, Andrew, and Julia Jones. "Developments in in situ hybridisation." Methods 70, no. 1 (November 2014): 39–45. http://dx.doi.org/10.1016/j.ymeth.2014.04.006.

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Warford, A., and I. Lauder. "In situ hybridisation in perspective." Journal of Clinical Pathology 44, no. 3 (March 1, 1991): 177–81. http://dx.doi.org/10.1136/jcp.44.3.177.

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Ekong, Rosemary, and Jonathan Wolfe. "Advances in fluorescent in situ hybridisation." Current Opinion in Biotechnology 9, no. 1 (February 1998): 19–24. http://dx.doi.org/10.1016/s0958-1669(98)80079-7.

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Chiecchio, Laura. "In situ hybridisation in tissue sections." Diagnostic Histopathology 26, no. 11 (November 2020): 521–28. http://dx.doi.org/10.1016/j.mpdhp.2020.08.005.

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Edwards, A. A. "Editorial - Fluorescence In Situ Hybridisation (FISH)." Radiation Protection Dosimetry 88, no. 1 (March 1, 2000): 5–6. http://dx.doi.org/10.1093/oxfordjournals.rpd.a033019.

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Morey, Adrienne L. "In situ hybridisation: background and applications." Pathology 43 (2011): S10. http://dx.doi.org/10.1016/s0031-3025(16)33100-2.

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Terenghi, Giorgio, and Julia M. Polak. "Morphological studies using in situ hybridisation." European Journal of Cancer and Clinical Oncology 27, no. 6 (June 1991): 785–89. http://dx.doi.org/10.1016/0277-5379(91)90190-o.

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Yao, Jin, Xingmei Li, Na Wu, Songlin Zhang, Min Gao, and Xiping Wang. "Improvement of RNA In Situ Hybridisation for Grapevine Fruits and Ovules." International Journal of Molecular Sciences 24, no. 1 (January 2, 2023): 800. http://dx.doi.org/10.3390/ijms24010800.

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The European grapevine (Vitis vinifera L.) is one of the world’s most widely cultivated and economically important fruit crops. Seedless fruits are particularly desired for table grapes, with seedlessness resulting from stenospermocarpy being an important goal for cultivar improvement. The establishment of an RNA in situ hybridisation (ISH) system for grape berries and ovules is, therefore, important for understanding the molecular mechanisms of ovule abortion in stenospermocarpic seedless cultivars. We improved RNA in situ hybridisation procedures for developing berries and ovules by targeting two transcription factor genes, VvHB63 and VvTAU, using two seeded varieties, ‘Red Globe’ and ‘Pinot Noir’, and two seedless cultivars, ‘Flame Seedless’ and ‘Thompson Seedless’. Optimisation focused on the time of proteinase K treatment, probe length, probe concentration, hybridisation temperature and post-hybridisation washing conditions. The objectives were to maximise hybridisation signals and minimise background interference, while still preserving tissue integrity. For the target genes and samples tested, the best results were obtained with a pre-hybridisation proteinase K treatment of 30 min, probe length of 150 bp and concentration of 100 ng/mL, hybridisation temperature of 50 °C, three washes with 0.2× saline sodium citrate (SSC) solution and blocking with 1% blocking reagent for 45 min during the subsequent hybridisation. The improved ISH system was used to study the spatiotemporal expression patterns of genes related to ovule development at a microscopic level.
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Shastry, Anjali, Preetha Tilak, and Amudha Subramaniam. "APPLICATION OF KARYOTYPING AND FLOURESCENT IN SITU HYBRIDISATION IN DETECTION OF KLINEFELTER SYNDROME." International Journal of Anatomy and Research 6, no. 3.3 (September 5, 2018): 5682–85. http://dx.doi.org/10.16965/ijar.2018.310.

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Dissertations / Theses on the topic "In-situ hybridisation"

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Princivalle, Alessandra Patrizia. "Studies of GABAb receptors in epilepsy." Thesis, University of Birmingham, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.289300.

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The binding of a novel GABAs receptor radioligand eH]-CGP62349 to human hippocampal control and epileptic sections was investigated using quantitative receptor autoradiography. Kinetic analyses performed on rat brain sections, to conserve the use of human tissues, demonstrate that eH]-CGP62349 associated rapidly. The same radioligand dissociated rapidly initially, then very slowly. Utilising human hippocampus it was shown that CH]-CGP62349 bound with high affinity (O.SnM) to human control hippocampal sections. The kinetics of GABAs receptor in human hippocampus using the novel compound confirmed previous studies performed in rat membrane. The localisation of GABAB receptors in human hippocampal control partially supported former studies using agonist ligands such as CH]-GABA and eH]-baclofen, despite differences have been noticed. Hippocampal slices from surgical resected specimens obtained from hippocampal sclerotic (HS)/temporal lobe epilepsy (TLE) patients were compared with neurologically normal post-mortem control subjects for neuropathology and GABAs receptor density and affinity. Neuronal loss was observed in most of the hippocampal subregions, whereas in subiculum any significant difference was detected. For GABAa receptor density (Bmax) a significant reduction was reported in CA3, CA4, and DG; the affinity was increased exclusively in DG. After the correction of Bmax value for the neuronal loss a significant increase was seen in CA3. Oligonucleotides were designed to investigate the two GABAB1 isoforms (la, and lb), and the GABAa2 subunit in human hippocampal control and HSITLE tissues, obtained as well as for the autoradiography. GABAsla, GABAB1b, and GABAB2 transcript distribution was in agreement with the receptor protein localisation, even though in the human hippocampus GABAa2 has to be yet localised and to verify if it is associated with GABAsla or GABABlbto form the dimeric active receptor. The present study suggests an involvement of GABABreceptors in HS/TLE in some not all the hippocampal subregions in terms of receptor density and affinity. Further investigations in regard to the quantitative in situ hybridisation data and the immunocytochemical results are fundamental to gain insight into the pathological role of GABAs receptors.
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Hoyle, Jane Anthea. "In situ hybridisation for the detection of viral nucleic acids." Thesis, University of St Andrews, 1991. http://hdl.handle.net/10023/13924.

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The technique of in situ hybridisation was optimised for the detection of viral RNA using radioactively-labelled single-stranded DNA and RNA probes, and applied to three areas of interest. Optimum hybridisation conditions were determined in vitro using cells infected with the single-stranded negative sense RNA paramyxoviruses. Transcription of RNA probes was the most rapid and efficient method of probe labelling, since electrophoretic purification was not required and large amounts of RNA were produced. However, their use for in situ hybridisation was problematic due to RNase contamination and low sensitivity. In contrast, DNA probes produced from M13 clones and oligonucleotide probes gave consistent hybridisation results and were preferred in subsequent studies for their ease of use, stability and sensitivity. The effect of virus-host interactions on the clearance of the paramyxovirus, SV5, in a mouse model was investigated by detection of viral RNA and protein in lung sections. Immunisation with purified SV5 proteins prior to infection provided protection against infection, indicated by a reduction in the level of viral RNA and protein, due to enhanced clearance of virus by primed T cells. X-irradiation of the host prior to infection resulted in prolonged or persistent infection in which RNA was detected up to 19 days post-infection. The potential of in situ hybridisation for detection of aetiological agents was demonstrated by investigation of the presence of measles virus in two chronic human diseases. Thus, measles virus RNA was detected in brain sections from a patient with subacute sclerosing panencephalitis and in the osteoclasts of bone sections from a patient with Paget's disease of bone. In situ hybridisation was used to analyse expression of the two immediate-early genes of herpesvirus saimiri, the 52K gene and the hinG gene. Differential expression was detected by hybridisation to mRNA using oligonucleotide probes, in productively-infected cells. The 52K gene was expressed asynchronously throughout the population in agreement with immunocytochemical detection of the 52K protein. In contrast, the hinG gene was expressed synchronously, with all cells showing similar levels of hybridisation, indicating a specific control mechanism for expression of the 52K gene, which differs from that of the hinG gene in requiring or being inhibited by additional factors. This may have relevance to the mechanism of establishment of latency in this virus.
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Hauxwell, Angela Jane. "In situ hybridisation for studying embryo development in Pisum sativum L." Thesis, University of East Anglia, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.279676.

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HajMohammadi, Sassan. "Development of FISH technology in pathological tissue." Thesis, University of Southampton, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.284578.

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Wu, Yih-Yiing. "Response of skin to noxious stimuli : studies using in situ hybridisation." Thesis, University of Newcastle Upon Tyne, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.263124.

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Birchall, Philip Simon. "Multicolour fluorescence in situ hybridisation to RNA in whole-mount Caenorhabditis elegans." Thesis, University of Cambridge, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.296668.

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Zheng, Yun-Ling. "Rapid prenatal diagnosis of common fetal aneuploidies by fluorescence in situ hybridisation." Thesis, University of Cambridge, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.318418.

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Teo, Chong Gee. "Analysis of the Epstein-Barr virus-host relationship by in situ hybridisation." Thesis, Imperial College London, 1989. http://hdl.handle.net/10044/1/47684.

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Mohaddes, Ardebili Seyed Mojtaba. "Optimisation of interphase fluorescence in situ hybridisation for detection of common aneuploidies." Thesis, Connect to e-thesis, 1996. http://theses.gla.ac.uk/692/.

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Thesis (Ph.D.) - University of Glasgow, 1996.
Ph.D. thesis submitted to the Faculty of Medicine, Department of Division of Developmental Medicine, University of Glasgow, 1996. Includes bibliographical references: p. 118-132. Print version also available.
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10

Hammond, David William. "Analysis of non-Hodgkin's lymphoma by conventional cytogenetics and fluorescence in-situ hybridisation." Thesis, University of Sheffield, 1995. http://etheses.whiterose.ac.uk/3064/.

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Cytogenetic analysis was performed on 40 non-Hodgkin's lymphoma (NHQ node biopsies. Chromosomes X, 3 and 12 were the most frequently gained; of the much rarer monosomies, loss of chromosome 13 was most common. Structural abnormalities primarily involved chromosomes 14,1,18,6 and 17. A markedly greater number of chromosome gains were associated with low-grade disease when compared to high-grade. In order to obtain further information from the cytogenetic analysis of the NHL karyotypes, the fluorescence in-situ hybridisation (FISH) technique was applied to the series. The activation state of additional X-chromosomes was examined and evidence that more than one X-chromosome was present in the active state in 4/9 cases was obtained. Further, in an apparent case of monosomy X, a marker was identified as an abnormal X-chromosome by chromosome painting. Interphase FISH was applied to NHL cells and numerical chromosome changes were identified; this approach was also attempted on aged bone marrow smears from acute lymphocytic leukaemia patients, in order to test the utility of the technique on archival material. Dual chromosome painting was used to elucidate the origins of add(14) chromosomes in 8 of the cases. In the control and two other cases the translocated material was demonstrated to be from chromosome 18, in two cases it was from chromosome 3 and in one case them was an insertion of chromosome 11 material. it was not possible to identify the origins of the translocated material in one NHL and in the final case the apparent add(14) was demonstrated not to contain chromosome 14 material. Structural abnormalities of chromosome 6 were investigated both by chromosome painting and by hybridisation of the MYB gene. The latter, which was initially mapped to 6q23 before hybridisation to NHL cells revealed previously unsuspected rearrangements. One case contained extrachromosomal chromatin bodies that appeared to be double minute chromosomes (dmin), which FISH analysis demonstrated to be derived from the X-chromosome and contain centromere-associated DNA. The significance of these results is discussed with reference to previously published series of NHL karyotypes.
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Books on the topic "In-situ hybridisation"

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Stevenson, Mark. Development of in situ hybridisation for cytogenetical analysis in allium. Birmingham: University of Birmingham, 1998.

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2

Hauxwell, Angela Jane. In situ hybridisation for studying embryo development in 'Pisum sativum L.'. Norwich: Universityof East Anglia, 1990.

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3

Jordan, Richard. A study of c-erbB-2 oncogene expression in salivary gland neoplasms by in situ hybridisation. Ottawa: National Library of Canada = Bibliothèque nationale du Canada, 1993.

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4

Triningsih, Ediati. Expression of transforming growth factor alpha and beta mRNA in cervical neoplasia by in situ hybridisation. Manchester: University of Manchester, 1993.

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Jordan, Richard. A study of c-erb B-2 oncogene expression in salivary gland neoplasms by in situ hybridisation. [Toronto: Faculty of Dentistry, University of Toronto], 1992.

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Goldman, Alastair Simon Howard. The segregation of normal and translocated chromosomes in human male meiosis: Analysis utilizing fluorescent in situ hybridisation. Birmingham: University of Birmingham, 1993.

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Bredin, Linda. Detection of HPV by in-situ hybridisation: Optimisation and introduction into the routine histology laboratory. [S.l: The Author], 2003.

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8

O'Keeffe, Christine. Analysis of the initiation and progression of homologous chromosome pairing at meiosis in the female mouse using fluorescence in situ hybridisation. Birmingham: University of Birmingham, 1997.

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9

Rees, Sally Anne. Fluorescence in situ hybridisation (FISH) to determine aneuploidy for chromosomes 1 and 12 in the normal male germ-line and in men exposed to radiation. Birmingham: University of Birmingham, 1997.

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Bysouth, James Peter Norman. Physical mapping of 45S and 5S rDNA repetitive sequences to mitotic chromosomes of Brassica species by fluorescence in situ hybridisation. Birmingham: University of Birmingham, 2003.

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Book chapters on the topic "In-situ hybridisation"

1

Hayes, H., and B. Dutrillaux. "In Situ Hybridisation Techniques." In Techniques in Animal Cytogenetics, 69–83. Berlin, Heidelberg: Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-59711-4_3.

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Terenghi, G., and R. A. Fallon. "Techniques and Applications of In Situ Hybridisation." In Current Topics in Pathology, 289–337. Berlin, Heidelberg: Springer Berlin Heidelberg, 1990. http://dx.doi.org/10.1007/978-3-642-74668-0_7.

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Várallyay, Éva, and Zoltán Havelda. "Detection of microRNAs in Plants by In Situ Hybridisation." In MicroRNAs in Development, 9–23. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-083-6_2.

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Rouquette, Jacques, Karl-Henning Kalland, and Stanislav Fakan. "Visualisation of RNA by Electron Microscopic In Situ Hybridisation." In The Nucleus, 403–13. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-461-6_22.

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Fleming, A. J. "Localization of RNA Transcripts in Plant Tissue by In Situ Hybridisation." In Gene Transfer to Plants, 273–85. Berlin, Heidelberg: Springer Berlin Heidelberg, 1995. http://dx.doi.org/10.1007/978-3-642-79247-2_27.

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Dey, Pranab. "Fluorescent In Situ Hybridisation Techniques in Pathology: Principle, Technique and Applications." In Basic and Advanced Laboratory Techniques in Histopathology and Cytology, 229–39. Singapore: Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-19-6616-3_21.

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Egger, Bernhard. "Studying Xenacoelomorpha WBR Using Isodiametra pulchra." In Methods in Molecular Biology, 245–61. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2172-1_13.

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AbstractXenacoelomorpha are a phylogenetically and biologically interesting, but severely understudied group of worm-like animals. Among them, the acoel Isodiametra pulchra has been shown to be amenable to experimental work, including the study of stem cells and regeneration. The animal is capable of regenerating the posterior part of the body, but not its head. Here, methods such as nucleic acid extractions, in situ hybridisation, RNA interference, antibody and cytochemical stainings, and the general handling of the animals are presented.
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Darby, Ian A., Alexis Desmoulière, and Tim D. Hewitson. "Using In Situ Hybridisation to Localize Renal Gene Expression in Tissue Sections." In Methods in Molecular Biology, 119–32. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-352-3_9.

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Noble, D. P., J. C. Lewthwaite, J. Dudhia, S. Blake, B. Henderson, and T. E. Hardingham. "In Situ Hybridisation for Decorin mRNA in Rabbit Syniovium During Antigen-Induced Arthritis." In Joint Destruction in Arthritis and Osteoarthritis, 249–53. Basel: Birkhäuser Basel, 1993. http://dx.doi.org/10.1007/978-3-0348-7442-7_30.

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Hijri, Mohamed. "The use of Fluorescent in situ Hybridisation in Plant Fungal Identification and Genotyping." In Plant Pathology, 131–45. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-062-1_11.

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Conference papers on the topic "In-situ hybridisation"

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Jia, Jianguo, Nima Sayyadi, Yan Wang, Honghua Hu, Karen Vickery, and Yiqing Lu. "Lifetime-Multiplexed Luminescence in situ Hybridisation for Bacteria Detection." In Conference on Lasers and Electro-Optics/Pacific Rim. Washington, D.C.: OSA, 2020. http://dx.doi.org/10.1364/cleopr.2020.p3_10.

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Song, Tzu-Hsi, Gabriel Landini, Shereen Fouad, and Hisham Mehanna. "Epithelial Segmentation From In Situ Hybridisation Histological Samples Using A Deep Central Attention Learning Approach." In 2019 IEEE 16th International Symposium on Biomedical Imaging (ISBI). IEEE, 2019. http://dx.doi.org/10.1109/isbi.2019.8759384.

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Chauhan, Hemma, Vasilica Plaiasu, Ozunu Diana, and Motei Gabriela. "P59 Clinical diversity in 22q11.2 microdeletion syndrome & difficulties in diagnoses using only fluroscence in situ hybridisation (fish)." In 8th Europaediatrics Congress jointly held with, The 13th National Congress of Romanian Pediatrics Society, 7–10 June 2017, Palace of Parliament, Romania, Paediatrics building bridges across Europe. BMJ Publishing Group Ltd and Royal College of Paediatrics and Child Health, 2017. http://dx.doi.org/10.1136/archdischild-2017-313273.147.

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Ali, Asif, Elisa Giovannetti, Niccola Funel, Roderick Ferrier, Jennifer Morton, and Karin A. Oien. "Abstract 2860: miR-21 overexpression assessed by in situ hybridisation is an independent predictor of survival in patients with resected pancreatic ductal adenocarcinoma." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-2860.

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Reiner-Concin, AM, S. Lax, P. Regitnig, C. Kronberger, Z. Jasarevic, and S. Bogner. "P5-11-15: Should HER-2 Score 0/1+ Breast Cancer Cases Be Retested by In-Situ Hybridisation? Results of a Multicenter Retesting Study." In Abstracts: Thirty-Fourth Annual CTRC‐AACR San Antonio Breast Cancer Symposium‐‐ Dec 6‐10, 2011; San Antonio, TX. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/0008-5472.sabcs11-p5-11-15.

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