Dissertations / Theses: 'Immunological Analyses'

1

Tran, Emily. "Immunological and molecular analyses of the Borrelia burgdorferi OSPF protein family /." Also available to VCU users at:, 2006. http://hdl.handle.net/10156/1917.

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Parry, David Alun. "Biochemical and functional analyses of p16INK4a, an inhibitor of cyclin D-dependent kinases." Thesis, Imperial College London, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243499.

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3

Vemulapalli, Tracy H. "Genetic and Immunological Analyses of a Brucella abortus Protein Exhibiting Lectin-like Properties." Thesis, Virginia Tech, 2000. http://hdl.handle.net/10919/31194.

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Brucella abortus is a facultative, intracellular zoonotic pathogen, which can cause undulant fever in humans and abortion in cattle. Despite all of the progress in brucellosis research, there are still many unanswered questions regarding the molecular mechanisms involved in the pathogenesis of Brucella infections. To better understand the Brucella antigens involved in virulence and/or immunity, genetic and immunologic characterization of a 16 kDa protein of B. abortus was performed. Using PCR methods, the gene encoding the 16 kDa protein was cloned and sequenced. PCR and Southern blot analysis revealed that the gene is conserved among the 6 nomen species of Brucella. Overexpression of this protein in B. abortus vaccine strain RB51 was achieved using Brucella groE and sodC promoters as well as its own promoter. Protection and clearance studies were performed in mice to determine the role of this protein in Brucella immunity and pathogenesis. Inoculation with either strain RB51 overexpressing the 16 kDa protein or a DNA vaccine encoding the 16 kDa protein gene failed to provide significant protection. No difference was noted between the splenic clearance of B. abortus strain 2308 and its recombinant overexpressing the 16 kDa protein. A mutant of strain 2308 (2308D16) was created by disrupting the 16 kDa proteinâ s gene with a chloramphenicol resistance cassette. Western blot analysis indicated that the O antigen profile of strain 2308D16 differed from that of strain 2308. Mice cleared strain 2308D16 faster than strain 2308 indicating the potential attenuation of the disruption mutant. Purified 16 kDa protein was obtained by overexpressing it in E. coli via the pRSET expression system. Western blotting results initially identified this protein as an immunoglobulin-binding protein. Hemagglutination assay revealed that the 16 kDa protein exhibits lectin-like properties. Preliminary studies using hemagglutination inhibition identified mannose as a possible sugar to which the 16 kDa protein can interact. The lectin-like properties exhibited by the 16 kDa protein appears to influence smooth lipopolysaccharide production, and thereby may be involved in virulence.
Master of Science

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Matthews, Kerryn. "Immunological analysis of pericardial tuberculosis." Doctoral thesis, University of Cape Town, 2011. http://hdl.handle.net/11427/11101.

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Includes bibliographical references (leaves 297-327).
Pericardial tuberculosis (TB) offers a relevant human model to study TB at the site of disease and to determine the effect of HIV-1 infection. 96 Patients with pericardial TB were recruited into this study, 68 of whom were HIV-1 infected. Where clinically indicated, pericardiocentesis was performed to drain pericardial fluid and blood was drawn. The data derived from the study provide novel insight into the immune response in the pericardium to TB infection. Furthermore, HIV-1 infection caused a dysregulation of the immune response.

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5

Montano, C. X. "Immunological analysis of SV40 early region products." Thesis, Imperial College London, 1985. http://hdl.handle.net/10044/1/37789.

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Gonzalez-Rabade, N. "Immunological analysis of chloroplast-derived HIV-1 antigens." Thesis, University of Cambridge, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.599489.

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The aim of this project was to assess the expression, purification and immunogenicity in mice of two important HIV antigens, the core protein p24 and the regulatory protein Nef, produced in transplastomic Nicotiana tabacum plants. A comparison of p24-expressing tobacco Petite Havana transplastomic plants, containing gene constructs with different 5’ untranslated regions (UTRs), demonstrated the importance of regulatory and stabilizing elements. Plants containing a p24 construct with a bacteriophage T7 gene 10 (T7g10) 5’ UTR and generating a protein with 5 extra amino acids at the N-terminus, accumulated almost twice as much protein as plants containing other constructs. Analysis of p24 accumulation in leaves of the high biomass tobacco variety Maryland Mammoth revealed a dramatic difference between a transplastomic line containing a codon-optimized construct encoding five extra N-terminal amino acids, which accumulated p24 in all leaves of the plant, and a transplastomic line containing a native p24 construct which accumulated p24 only in young leaves. To assess the subcutaneous and nasal immunogenicity of plant-derived p24, the protein was purified to ~95% homogeneity from Maryland Mammoth transplastomic plants. After immunization of experimental mice, ELISAs for p24-specific IgG and T-cell proliferation assays showed that chloroplast p24 could elicit a strong humoral response following subcutaneous administration and a cellular response following intranasal administration. Oral administration of partially purified tobacco p24-Nef as a booster, after subcutaneous priming with either p24 or Nef, elicited strong humoral and cellular responses, with both IgG1 and IgG2 subtypes found in sera. In addition, tobacco BiP was found to be as good an adjuvant as Vibrio cholerae toxin B and Mycobacterium tuberculosis Hsp70 for enhancing the immune response to p24.

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CELNIKER, ABBIE CHERYL. "IMMUNOLOGICAL STUDIES OF HUMAN CATHEPSIN D (LYSOSOMAL ENZYME, ACID PROTEINASE)." Diss., The University of Arizona, 1986. http://hdl.handle.net/10150/183856.

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Cathepsin D is a lysosomal acid proteinase that exists as different isoforms. This enzyme is found in most cell types, and macrophages have particularly high levels of this enzyme. Cathepsin D has been implicated in some disease related inflammatory processes that have also been associated with increased macrophage infiltration. The existance of isoforms of cathepsin D suggested the possibility that individual forms of this enzyme may have immunologically unique characteristics. Monoclonal antibodies to cathepsin D could aid in the study of the immunological characteristics of individual isoforms of this enzyme as well as provide a means by which to screen a variety of sample types for cathepsin D levels. In this study we generated a panel of monoclonal anti-cathepsin D antibodies and used these to screen isoelectrically separated cathepsin D samples for unique immunoreactivity patterns. We also used these antibodies to measure changes in the levels of intra- and extracellular cathepsin D that accompany monocyte to macrophage differentiation, and changes that occur in response to the treatment of melanoma cells with tumor necrosis factor (TNF) and interferon. We found that there are unique immunological characteristics associated with individual isoforms of human liver cathepsin D as well as cathepsin D from different cell types. We also observed increases in levels of this enzyme as monocytic cells differentiate to macrophage-like cells. This indicates that cathepsin may be involved in some of the proteolytic processes mediated by macrophages. Different inducers of differentiation resulted in different cathepsin 1D immunoreactivity patterns, suggesting that there may be isoform specificity dependent on the inducer. Cathepsin D levels also increase in neoplastic cells treated with TNF and/or interferon indicating that cathepsin D may play some role in the mechanism of action of the tumor inhibitory effects of these agents. The use of monoclonal antibodies provides assays for cathepsin D which can be used with a variety of sample types and further provides enhanced specificity and sensitivity relative to other assay systems used in the past.

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Elliott, Michael. "Immunological, virological and molecular biological analysis of nasopharyngeal carcinoma /." [St. Lucia, Qld.], 2004. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe18427.pdf.

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9

Ross, Owen A. "Analysis of genetic and immunological factors associated with ageing." Thesis, University of Ulster, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.274056.

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Odendaal, Ruenda. "A biochemical and immunological study of horseradish peroxidase." Thesis, Stellenbosch : University of Stellenbosch, 2009. http://hdl.handle.net/10019.1/1587.

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Thesis (MSc (Biochemistry))--University of Stellenbosch, 2009.
ENGLISH ABSTRACT: This study describes: a) the isolation and purification of horseradish peroxidase isoenzymes from horseradish roots, b) the characterization of various forms and components of the enzyme by cation-exchange and reversed-phase high performance liquid chromatography, c) the preparation of antibodies against horseradish peroxidase isoenzymes, d) immunological studies for the development of an isoenzyme quantification method and e) the formation of an enzyme-melamine conjugate for use in a melamine quantification immunoassay.
AFRIKAANSE OPSOMMING: Hierdie studie beskryf: a) die isolering en suiwering van peperwortel-peroksidase-isoënsieme vanuit die peperwortel, b) die karakterisering van verskillende vorme en komponente van dié ensiem deur katioonuitruilings en omgekeerde-fase HPLC c) die voorbereiding van teenliggaampies vir peperwortel-peroksidase-isoënsieme, d) immunologiese studies vir die ontwikkeling van 'n isoënsiem-kwantifiseringsmetode; en e) die vorming van 'n ensiem-melamien-konjugaat vir gebruik in 'n melamienkwantifiseringsmetode.

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11

Cavar, Marko. "Mutational Analysis of CD127 and Its Role in Immunological Diseases." Thesis, Université d'Ottawa / University of Ottawa, 2016. http://hdl.handle.net/10393/34404.

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Interleukin (IL) -7 is an essential non-redundant cytokine that influences T-cell differentiation, proliferation, homeostasis and T-cell functions. In T-cells, IL-7 signals are transduced via IL-7's heterodimeric receptor composed of a common, γ chain (CD132) and an IL-7 specific, α chain (CD127). In light of the many roles that IL-7 plays in T-cell biology, it is no surprise that CD127 expression is tightly regulated in T-cells. In this study, I explore the effects that disease specific mutations in CD127 have on CD127 expression, regulation and signal transduction using an in vitro T-cell model. Here I specifically examined four disease associated mutations of CD127: P132S associated with severe combined immunodeficiency; L242_L243insNPC associated with T-cell acute lymphoblastic leukemia; I356V & T244I associated with autoimmune diseases like multiple sclerosis, rheumatoid arthritis and type 1 diabetes. In developing my model, I decided to use Jurkat cells because they expressed high endogenous surface levels of CD132, low endogenous surface levels of CD127 and endogenous STAT5. Jurkat cells were transduced with lentiviruses that induced expression of either WT or one of the four mutant CD127. I found that transduced Jurkat cells produced the WT and all four mutant CD127 proteins. I also found that wild type CD127, I356V, L242_L243insNPC and T244I mutant CD127 proteins were all expressed at the same level on the cell surface. However, I could not detect P132S mutated CD127 protein in its native state on the surface or intracellularly. I also found no differences between the mutant CD127 and wild type CD127 with regards to the level of soluble CD127 transcripts. I found that cell lines expressing L242_L243insNPC, I356V and T244I mutant CD127 protein, down-regulated surface CD127 at high IL-7 doses (25ng/mL) to the same extent as in the cell line expressing wild type CD127 protein. Interestingly, at the low IL-7 dose (1ng/mL) these mutant CD127 cell lines down-regulated surface CD127 to a lesser degree the wild type CD127 cell line. Further studies are required to elucidate whether P132S mutated CD127 is expressed on the surface and if T224I and I356V mutations in CD127 enhance signaling. By understanding CD127 dysregulation and dysfunction in disease states, we can potentially develop therapeutics that can return the function of CD127 to normalcy.

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12

Hassan, Syed Saeed-Ul. "Rapid immunological methods for analysis of dexamethasone in equine urine." Thesis, University of Sunderland, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.245822.

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McLean, William Henry Irwin. "Electrophoretic and immunological analysis of proteins in the muscular dystrophies." Thesis, Queen's University Belfast, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335992.

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Cox, Peter John. "Molecular and immunological analysis of EHV-1 and -4 infections." Thesis, University of Glasgow, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.285022.

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15

Adeleye, Tolulope Abiodun. "A molecular analysis of the immunological response to mycobacterial antigens." Thesis, University College London (University of London), 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.304066.

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16

Mann, Aisling M. "Immunological and biochemical techniques in the analysis of tear proteins." Thesis, Aston University, 1998. http://publications.aston.ac.uk/9598/.

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This study is concerned with the analysis of tear proteins, paying particular attention to the state of the tears (e.g. non-stimulated, reflex, closed), created during sampling, and to assess their interactions with hydrogel contact lenses. The work has involved the use of a variety of biochemical and immunological analytical techniques for the measurement of proteins, (a), in tears, (b), on the contact lens, and (c), in the eluate of extracted lenses. Although a diverse range of tear components may contribute to contact lens spoilation, proteins were of particular interest in this study because of their theoretical potential for producing immunological reactions. Although normal host proteins in their natural state are generally not treated as dangerous or non-self, those which undergo denaturation or suffer a conformational change may provoke an excessive and unnecessary immune response. A novel on-lens cell based assay has been developed and exploited in order to study the role of the ubiquitous cell adhesion glycoprotein, vitronectin, in tears and contact lens wear under various parameters. Vitronectin, whose levels are known to increase in the closed eye environment and shown here to increase during contact lens wear, is an important immunoregulatory protein and may be a prominent marker of inflammatory activity. Difficulties arise when attempting to extract proteins from the contact lens in order to examine the individual nature of the proteins involved. These problems were partly alleviated with the use of the on-lens cell assay and a UV spectrophotometry assay, which can analyse the lens surface and bulk respectively, the latter yielding only total protein values. Various lens extraction methods were investigated to remove protein from the lens and the most efficient was employed in the analysis of lens extracts. Counter immunoelectrophoresis, an immunodiffusion assay, was then applied to the analysis of albumin, lactoferrin, IgA and IgG in the resultant eluates.

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Han, Qing Ph D. Massachusetts Institute of Technology. "Generating immunological signatures of diseases by multiplex analysis of single cells." Thesis, Massachusetts Institute of Technology, 2012. http://hdl.handle.net/1721.1/76485.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 2012.
Cataloged from PDF version of thesis.
Includes bibliographical references (p. 135-142).
The large diversity of cells that comprise the human immune system requires methods that can resolve the contributions of individual cells to an immunological response. The release of cytokines is one of several important functions carried out by immune cells. Analytical methods that yield multiple measures of the breadth and quality of cytokine secretion from heterogeneous populations are highly desired in basic and clinical immunology. Microengraving is a process that uses a dense, elastomeric array of nanowells to generate microarrays of proteins secreted from large numbers of individual live cells. In this thesis, we improved the sensitivity and multiplicity of microengraving and adapted it to detect cytokine secretions from primary immune cells. We demonstrated that microengraving could provide quantitative measurements of both the frequencies and the distribution in rates of secretion for up to four cytokines simultaneously. The experimental limits of detection ranged from 0.5 to 4 molecules/s for most cytokines in our experiments. These multidimensional measures resolve functional responses by cells exposed to stimuli with greater sensitivity than single-parameter assays. Primary T cells with specific profiles of secretion can also be recovered after microengraving for subsequent expansion in vitro. The release of multiple cytokines by T cells has been associated with beneficial immune responses. To date, however, time-integrated end-point measurements have not resolved the temporal dynamics of these functions. Here, we used serial microengraving to measure Thlskewed cytokine responses (IFN[gamma], IL-2, TNF[alpha]) from individual cells after activation ex vivo. The results show that multifunctional cytokine responses are initiated asynchronously but the ensuing dynamic trajectories of these responses evolve programmatically in a sequential manner. Furthermore, these dynamic trajectories are strongly associated with the various states of cell differentiation, suggesting that transient programmatic activities of many individual T cells contribute to sustained, population-level responses. The trajectories of responses by single cells may also provide unique, time-dependent signatures for immune monitoring that are less compromised by the timing and duration of integrated measures. Together, these results demonstrate the utility of quantitative, multidimensional profiles of single cells for analyzing the diversity and dynamics of immune responses in vitro, thus generating immune signatures for diseases.
by Qing Han.
Ph.D.

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18

Örtqvist, Eva. "The importance of immunological, genetic and clinical factors for beta cell function in childhood diabetes /." Stockholm, 2001. http://diss.kib.ki.se/2001/91-628-4632-9/.

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Reid, Helen Isobel. "Molecular and immunological analysis of recombinant Bacteroides forsythus heat shock protein 60." Thesis, University of Glasgow, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321982.

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Zal, Behnam. "Biosynthesis and immunological analysis of the dominant phosphoprotein ppUL83 of human cytomegalovirus." Thesis, St George's, University of London, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.313517.

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21

Galbraith, Daniel Norman. "Molecular and immunological analysis and detection of the forest pathogen Heterbasidion annosum." Thesis, University of Abertay Dundee, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.359300.

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22

Roßmann, Laura [Verfasser]. "In-depth immunological analysis of prototypic adjuvants for vaccine formulations / Laura Roßmann." Mainz : Universitätsbibliothek der Johannes Gutenberg-Universität Mainz, 2020. http://d-nb.info/1223379299/34.

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23

Wilson, Lisa. "Immunological analysis of drugs of abuse with reference to anhydroecgonine methyl ester." Thesis, Sheffield Hallam University, 2007. http://shura.shu.ac.uk/20704/.

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The field of forensic drug testing is continually changing with analytical methodology being developed for an increasing number of drugs in a variety of biological matrices. The aim of this thesis was to develop a novel enzyme immunoassay (EIA) for screening oral fluid specimens for the presence of anhydroecgonine methyl ester (AEME), a pyrolysis product of cocaine. A confirmatory method was also to be developed to accurately quantify the levels of cocaine, its metabolites and pyrolysis products in oral fluid samples. The immunoassay development was started by synthesising an immunogen using anhydroecgonine (AE) and thyroglobulin. Following immunisation the antisera were screened by enzyme linked immunosorbent assay (ELISA) to enable selection of the antibody with the highest specificity and sensitivity. An enzyme labelled drug was synthesised and the titres of antibody and enzyme were optimised. A series of validation experiments were carried out which concluded that the EIA was sensitive, highly specific, and precise. Gas chromatography-mass spectrometry (GC-MS) was investigated for the quantitation of cocaine and its metabolites. A temperature program was selected which allowed for the simultaneous analysis of all the analytes. A solid phase extraction (SPE) method was developed to extract cocaine and its metabolites from oral fluid. The SPE method provided high recovery for all analytes apart from the highly polar AE. Degradation of the GC column had a detrimental effect on the analysis of AEME, and so the confirmation method was switched to liquid chromatography-tandem mass spectrometry (LC-MS/MS). A series of LC columns and mobile phases were tested for optimum separation and ionisation. The instrument parameters such as capillary voltage, drying gas temperature, shield voltage, and needle voltage, were optimised. Following a number of validation experiments the method was found to be highly sensitive, precise, accurate and robust. Both the EIA and LC-MS/MS methods were applied to the analysis of clinical samples from self declared crack cocaine users. The EIA showed good correlation to LC-MS/MS. It was evident that the presence of AEME can positively identify smoking as the route of cocaine administration however its absence does not necessarily mean the individual has not smoked cocaine.

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Saville, Stephen Paul. "Analysis of yeast proteins with immunological or sequence similarities to mammalian PKC isozymes." Thesis, University of Leicester, 1998. http://hdl.handle.net/2381/30311.

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When this project began, its principal aims were the isolation and characterisation of additional members of the yeast PKC family, which were believed to exist on the basis of biochemical data obtained in our laboratory, and others worldwide. One such gene, PKC2, was identified in our laboratory (Simon et al., 93) and a thorough functional analysis of its respective peptide product, Pkc2p was to have formed a major part of this project. In November 1994, a paper was published which cast serious doubts on the authenticity of the PKC2 gene (Levin et al., 94). In order for this study to continue, it was necessary to verify the existence of PCK2 . Nucleotide sequencing of all the recombinants purportedly used to generate the PKC2 open reading frame failed to reveal any of the hallmark sequences which allowed its categorisation as a member of the PKC family. Nevertheless, the weight of biochemical evidence suggested that other PKC-like proteins did exist in yeast. In an attempt to identify the protein(s) responsible for these activities, an immunological screen was performed using polyclonal antisera known to recognise mammalian PKC isoforms. This screening failed to detect any such proteins but cross-reactivity was observed with the product of the YOR080w open reading frame. A thorough functional analysis of the YOR080w gene product, implicates the protein in the normal progression of the yeast cell cycle, with the defect manifesting itself at the spindle assembly checkpoint.

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Thilaganathan, Baskaran. "Investigation of fetal immunological development by flow cytometric analysis of circulating lymphocyte subpopulations." Thesis, King's College London (University of London), 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.244163.

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Manso-Silvan, Lucia. "Identification and analysis of immunologically relevant antigens from Mycoplasma hyopneumoniae." Thesis, Royal Veterinary College (University of London), 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.404983.

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27

Vacca, Irene <1985&gt. "Analysis of the immunological and functional features of the Neisserial Heparin Binding Antigen (NHBA)." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2014. http://amsdottorato.unibo.it/6490/.

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Neisserial Heparin Binding Antigen (NHBA) is a surface-exposed lipoprotein ubiquitously expressed by genetically diverse Neisseria meningitidis strains and is an antigen of the multicomponent protein-based 4CMenB vaccine, able to induce bactericidal antibodies in humans and to bind heparin-like molecules. The aim of this study is to characterize the immunological and functional properties of NHBA. To evaluate immunogenicity and the contribution of aminoacid sequence variability to vaccine coverage, we constructed recombinant isogenic strains that are susceptible to bactericidal killing only by anti-NHBA antibodies and engineered them to express equal levels of selected NHBA peptides. In these recombinant strains, we observed different titres associated with the different peptide variants. These recombinant strains were then further engineered to express NHBA chimeric proteins to investigate the regions important for immunogenicity. In natural strains, anti-NHBA antibodies were found to be cross-protective against strains expressing different peptides. To investigate the functional properties of this antigen, the recombinant purified NHBA protein was tested in in vitro binding studies and was found to be able to bind epithelial cells. The binding was abolished when cells were treated specifically with heparinase III, suggesting that the interaction with the cells is mediated by heparan sulfate proteoglycans (HSPG). Mutation of the Arg-rich tract of NHBA abrogated the binding, confirming the importance of this region in mediating the binding to heparin-like molecules. In a panel of N. meningitidis strains, the deletion of nhba resulted in a reduction of adhesion with respect to each isogenic wild type strain. Furthermore, the adhesion of the wild-type strain was prevented by using anti-NHBA polyclonal sera, demonstrating the specificity of the interaction. These results suggest that NHBA could be a novel meningococcal adhesin contributing to host-cell interaction. Moreover, we analysed NHBA NalP-mediated cleavage in different NHBA peptides and showed that not all NHBA peptides are cleaved.

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Glancy, Heather. "Detection and analysis of the wood decay fungus Lentinus lepidus Fr. using immunological probes." Thesis, University of Abertay Dundee, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.278052.

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Hageman, John Robert. "A morphometric and immunological analysis of kidney flask cells from the frog Xenopus laevis /." The Ohio State University, 1990. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487682558447011.

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Gure, Ali Osmay. "Analysis of TNF mediated cytotoxicity /." Access full-text from WCMC, 1995. http://proquest.umi.com/pqdweb?did=733006521&sid=10&Fmt=2&clientId=8424&RQT=309&VName=PQD.

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Padilla, Lacy Ann. "GROUNDSTONE ANALYSIS AT THE ROCK CAMP SITE." CSUSB ScholarWorks, 2017. https://scholarworks.lib.csusb.edu/etd/603.

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The use of mortar and pestles has long been associated with acorn processing in California. Based on ethnographic and archaeological evidence, groundstone was used to process a multitude of resources, including small mammals. Twenty groundstone artifacts recovered from the Rock Camp Site in the San Bernardino Mountains were analyzed for protein residues using the crossover immunological electrophoresis (CIEP) method. Using previously obtained data from the Summit Valley, a comparative analysis was done to determine if processing small mammals on groundstone was a common occurrence throughout the San Bernardino Mountain region.

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Teng, Yen-Tung Andy. "Analysis of the mechanism(s) of immunological tolerance to a physiological soluble antigen in transgenic mice." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/Nq27740.pdf.

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Eliasson, Hanna. "Development of immunological methods and Real-Time PCR for detection of Macadamia nut (Macadamia spp.)." Thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-6150.

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A new European labeling directive (2003/89/EC) states that certain foods and products derived thereof must always be declared. Among the tree nuts specified is Macadamia nut (Macadamia spp.). During the last few years, cases of IgE-allergic reactions, even severe anaphylaxes, have been reported. Reliable methods for the detection of this nut are needed.

Protein from Macadamia nuts was isolated. Polyacrylamide gel electrophoresis in SDS revealed two main protein bands of about 20 and 50kDa. These protein bands were cut and extracted from the gel and rabbits were immunized with each protein.

Immunoblotting showed dominant reactivity with the respective antigens. The antisera were further tested for specificity in immunodiffusion and in rocket immunoelectrophoresis.

In addition, a specific DNA-method was developed, based on Real-Time PCR using Macadamia vicilin as target sequence. Two different primer pairs were tested. Specificity was tested against potentially related nuts. Optimisation of primer and probe concentrations was performed. The limit of detection was 2-4 pg DNA, corresponding to a macadamia nut concentration of 50 to 100 μg per g. In a background of soybean DNA, down to 0,01 % macadamia DNA could be detected.

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Guy, Rebecca Ann. "Giardia lamblia : an analysis of trophozoite antigens using monoclonal antibodies." Thesis, McGill University, 1989. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=55696.

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Harder, Jutta [Verfasser], da Costa Clarissa [Akademischer Betreuer] Prazeres, Bettina [Gutachter] Kuschel, and da Costa Clarissa [Gutachter] Prazeres. "Vitamin D receptor and its immunological role within the human placenta - Analysis of vitamin D metabolism and immunologically important genes at the feto-maternal interface / Jutta Harder ; Gutachter: Bettina Kuschel, Clarissa Prazeres da Costa ; Betreuer: Clarissa Prazeres da Costa." München : Universitätsbibliothek der TU München, 2019. http://d-nb.info/1203799446/34.

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Matt, Muriel. "Concept et développement d'un immunodosage par polarisation de fluorescence de biomolécules à fonction carboxyle : application à la prostaglandine E1 et au thromboxane B2." Vandoeuvre-les-Nancy, INPL, 1993. http://docnum.univ-lorraine.fr/public/INPL_T_1993_MATT_M.pdf.

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Une des méthodes immunologiques pouvant être appliquée au dosage de biomolécules de faible masse moléculaire est la polarisation de fluorescence. Cette méthodologie a été appliquée aux biomolécules à fonction carboxyle telles que les éicosanoïdes, et en particulier au thromboxane B2, métabolite de la membrane plaquettaire impliqué dans l'hémostase et la thrombose. Les conditions opératoires d'amidification ont été optimisées après la synthèse et la purification du marqueur fluorescent, une fluorescéine fonctionnalisée par une fonction amine. La dérivation de la prostaglandine E1 (PGE1), et du thromboxane B2 (TxB2) a permis d'obtenir des traceurs fluorescents qui ont été purifiés par chromatographie sur couche mince. Leurs caractéristiques spectrales ont été déterminées. La conservation de l'immunréactivité a été vérifiée dans différents systèmes utilisant des marqueurs isotopiques ou enzymatiques. La praticabilité de l'immunodosage a été montrée pour la PGE1. La méthodologie a pu être transposée au TxB2. Le TxB2 libéré par les plaquettes après activation par un agent pro-agrégant a été dosé. Les résultats obtenus sont en accord avec les données bibliographiques

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Kolibab, Kristopher Adam. "Immunological and Molecular Analysis in Elderly and Young Adults in Response to Pneumococcal Polysaccharides 4 and 14." University of Toledo Health Science Campus / OhioLINK, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=mco1121869959.

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Chan, Agnes How-Ching. "Purification, biochemical analysis and sequencing of a novel murine T suppressor factor." Thesis, University of British Columbia, 1988. http://hdl.handle.net/2429/28638.

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The work reported in this thesis involved the purification, biochemical analysis and sequencing of a novel suppressor factor secreted by a T cell hybridoma, A10. The factor, A10F, isolated from spent medium of A10 cells, was found to consist of two forms with molecular weights at 140 - 160 and 80 kD as suggested by NH₂-terminal sequencing, Western blotting and tryptic peptide mapping experiments. Both forms of A10F were found to be capable of suppressing the in vitro generation of cytotoxic T lymphocyte (CTL) specific for P815 cells by syngeneic (DBA/2) splenocytes. In vitro ³⁵S methionine labeling experiments clearly demonstrated that the 80 kD protein was a secretory product of the A10 cells. The protein, which was specific to the monoclonal antibody (B16G), was absent in the control NS1 and BW5147 cells. Biochemical analysis indicated that the 80 kD molecule, was either a degradation product or a monomer of the 140 - 160 kD molecule. Further degradation products such as the 32 kD molecules were also found. This peptide, however, did not seem to cause substantial suppression in the in vitro CTL assay. When the 140 - 160, 80 and 32 kD proteins were sequenced at the NH₂ terminus, both 140 - 160 and 80 kD proteins were found to possess the same NH₂ terminus sequence. The 32 kD protein, on the other hand, was found to have an NH₂-terminus quite different from that of the 80 kD protein. These findings suggested that the 32 kD fragment was probably located at the distal end of the 140 - 160 kD molecule.
Science, Faculty of
Microbiology and Immunology, Department of
Graduate

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39

Gadd, Stephen J. "Analysis of acute mycloid leukaemia cell surface antigens with monoclonal antibodies." Title page, contents and abstract only, 1985. http://web4.library.adelaide.edu.au/theses/09PH/09phg123.pdf.

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Schmedt, Erich M. "An immunological analysis of a cell surface antigen in oocytes and embryos of the mud snail, Ilyanassa obsoleta /." Thesis, McGill University, 1985. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=63257.

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Fukazawa, Yoshinori. "Virological and immunological analysis of systemic lymphoid tissues in the early phase of simian-human immunodeficiency virus infection." Kyoto University, 2009. http://hdl.handle.net/2433/123950.

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Kyoto University (京都大学)
0048
新制・課程博士
博士(人間・環境学)
甲第14735号
人博第471号
新制||人||115(附属図書館)
20||人博||471(吉田南総合図書館)
UT51-2009-D447
京都大学大学院人間・環境学研究科相関環境学専攻
(主査)准教授三浦智行, 教授五十嵐樹彦, 教授小松賢志
学位規則第4条第1項該当

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Lähdesmäki, Aleksi. "Functional analysis of ATM with relevance for primary immunodeficiency and tumor formation /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7349-900-5/.

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Jin, Jing, and 金晶. "Proteomic profiling of mycelial extract derived from coriolus versicolor and analysis of their anti-tumor effects in human leukemiccells HL-60." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B41897110.

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Jin, Jing. "Proteomic profiling of mycelial extract derived from coriolus versicolor and analysis of their anti-tumor effects in human leukemic cells HL-60." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B41897110.

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Choi, Chang Won. "Soybean mosaic virus-soybean interactions : molecular, biochemical, physiological, and immunological analysis of resistance responses of soybean to soybean mosaic virus /." Diss., This resource online, 1991. http://scholar.lib.vt.edu/theses/available/etd-07282008-134858/.

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Ballenberger, Nikolaus Verfasser], and Erika von [Akademischer Betreuer] [Mutius. "Novel statistical approaches for censored immunological data : analysis of cytokine and gene expression data / Nikolaus Ballenberger. Betreuer: Erika von Mutius." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2012. http://d-nb.info/1031380388/34.

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Brunet, Jean-François. "Recherche et caractérisation des activités anticorps des liquides kystiques des tumeurs cérébrales." Université Joseph Fourier (Grenoble ; 1971-2015), 1994. http://www.theses.fr/1994GRE10222.

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Le but de cette these est d'etudier les reponses immunes humorales intracerebrales lors de processus tumoraux cerebraux. Elle comprend donc une introduction sur les interactions entre les systemes immunitaire et nerveux central d'une part et sur les tumeurs cerebrales d'autre part. Puis une presentation du materiel biologique donne notamment les raisons du choix des liquides kystiques pour cette etude immunologique. Le troisieme chapitre decrit les etudes qualitative et quantitative des immunoglobulines des liquides kystiques des tumeurs cerebrales. Les deux chapitres suivants presentent la recherche des activites anticorps des liquides kystiques dirigees respectivement contre le tissu cerebral non tumoral et contre les tissus cerebraux tumoraux, par immunohistochimie et par immunotransfert. Les effets des immunoglobines de classe g des liquides kystiques, sur des lignees cellulaires, sont decrits dans le sixieme chapitre. Puis les protocoles developpes pour la purification des antigenes reconnus par les liquides kystiques sont exposes, avant une discussion generale sur l'ensemble des resultats

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48

Maphumulo, Philile Nompumelelo. "Characterisation of a plasmodium falciparum type II Hsp40 chaperone exported to the cytosol of infected erythrocytes." Thesis, Rhodes University, 2013. http://hdl.handle.net/10962/d1015681.

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Heat Shock 40 kDa proteins (Hsp40s) partner with heat shock 70 kDa proteins (Hsp70s) in facilitating, among other chaperone activities; correct protein transport, productive protein folding and assembly within the cells; under both normal and stressful conditions. Hsp40 proteins regulate the ATPase activity of Hsp70 through interaction with the J-domain. Plasmodium falciparum Hsp70s (PfHsp70s) do not contain a Plasmodium export element (PEXEL) sequence although PfHsp70-1 and PfHsp70-3 have been located outside of the parasitophorous vacuole. Studies reveal that a type I P. falciparum (PfHsp40) chaperone (PF14_0359) stimulates the rate of ATP hydrolysis of the cytosolic PfHsp70 (PfHsp70-1) and that of human Hsp70A1A. PFE0055c is a PEXEL-bearing type II Hsp40 that is exported into the cytosol of P. falciparum-infected erythrocytes; where it potentially interacts with human Hsp70. Studies reveal that PFE0055c associates with structures found in the erythrocyte cytosol termed “J-dots” which are believed to be involved in trafficking parasite-encoded proteins through the erythrocyte cytosol. If P. falciparum exports PFE0055c into the host cytosol, it may be proposed that it interacts with human Hsp70, making it a possible drug target. The effect of PFE0055c on the ATPase activity of human Hsp70A1A has not been previously characterised. Central to this study was bioinformatic analysis and biochemical characterisation PFE0055c using an in vitro (ATPase assay) approach. Structural domains that classify PFE0055c as a type II Hsp40 were identified with similarity to two other exported type II PfHsp40s. Plasmids encoding the hexahistidine-tagged versions of PFE0055c and human Hsp70A1A were used for the expression and purification of these proteins from Escherichia coli. Purification was achieved using nickel affinity chromatography. The urea-denaturing method was used to obtain the purified PFE0055c whilst human Hsp70A1A was purified using the native method. PFE0055c could stimulate the ATPase activity of alfalfa Hsp70, although such was not the case for human Hsp70A1A in vitro.

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Scherr, Adolfo José de Oliveira 1979. "Terapia adjuvante pós tratamento cirúrgico no carcinoma renal : revisão sistemática da literatura com meta-análise." [s.n.], 2013. http://repositorio.unicamp.br/jspui/handle/REPOSIP/313866.

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Orientadores: André Deeke Sasse, Carmen Silvia Passos Lima
Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas
Made available in DSpace on 2018-08-22T03:20:05Z (GMT). No. of bitstreams: 1 Scherr_AdolfoJosedeOliveira_M.pdf: 1501111 bytes, checksum: acf22564245404a459e366435fc5790b (MD5) Previous issue date: 2013
Resumo: Pacientes com câncer renal localmente avançado são de alto risco para recidiva após ressecção cirúrgica com intuito curativo. Muitos estudos têm sido realizados na tentativa de se descobrir alguma intervenção adjuvante capaz de reduzir este risco. No entanto, até o momento não foi observado nenhum benefício clínico nas intervenções avaliadas nos estudos. O objetivo desta revisão sistemática foi avaliar o exato papel da terapia adjuvante nos pacientes com câncer renal localmente avançado após cirurgia. Foram selecionados estudos clínicos randomizados que comparavam terapia adjuvante (quimioterapia, vacinas, imunoterapia, bioquimioterapia, hormonioterapia) versus nenhum tratamento ativo após cirurgia em pacientes com câncer renal. Os desfechos clínicos avaliados foram sobrevida global (SG), sobrevida livre de doença (SLD) e toxicidades severas. A análise dos dados extraídos foi realizada no programa estatístico Review Manager 5.0 (RevMan 5; Cochrane Collaboration Software). As diferentes estratégias de tratamento adjuvante foram avaliadas em conjunto e separadamente. Dez estudos (2609 pacientes) foram incluídos. Terapia adjuvante não mostrou benefício em termos de SG (HR 1.07; IC95% 0.89 a 1.28; P = 0.48 I2= 0%) ou SLD (HR 0,96; IC95% 0.83 a 1.10; P =0.52 I2= 36%) quando comparado a nenhum tratamento adjuvante. Nenhuma análise de subgrupo (imunoterapia,vacinas, bioquimioterapia) atingiu resultado relevante. A avaliação de toxicidades mostrou uma frequencia significativamente maior de eventos adversos graves no grupo tratado (OR 73.86; IC 95% 28,32 a 192,62; P < 0,00001 I2 = 37%). O resultado final da análise não forneceu nenhum suporte para a hipótese de que os agentes estudados forneçam qualquer benefício clínico para pacientes com câncer renal no contexto adjuvante, além de aumentarem o risco de efeitos adversos graves. Estudos clínicos randomizados que avaliam o uso de terapias-alvo no cenário adjuvante estão em andamento e podem abrir uma nova fronteira terapêutica para estes pacientes. Até que os resultados destes estudos sejam conhecidos e se mostrem efetivos, nenhuma terapia adjuvante pode ser recomendada para pacientes com câncer de células renais após ressecção cirúrgica curativa
Abstract: Many adjuvant trials have been undertaken in an attempt to reduce the risk of recurrence among patients who undergo surgical resection for locally advanced renal cancer. However, no clear benefit has been identified to date. This systematic review was conducted to examine the exact role of adjuvant therapy in renal cancer setting. Randomized controlled trials were searched comparing adjuvant therapy (chemotherapy, vaccine, immunotherapy, biochemotherapy, hormone therapy) versus no active treatment after surgery among renal cell cancer patients. Clinical outcomes were overall survival (OS), disease-free survival (DFS), and severe toxicities. The extracted data was performed using the statistical software Review Manager 5.0 (RevMan 5; Cochrane Collaboration Software).Different strategies of adjuvant treatment were evaluated together and separately. Ten studies (2,609 patients) were included. Adjuvant therapy provided no benefits in terms of OS (HR 1.07; 95%CI 0.89 to 1.28; P = 0.48 I2 = 0%) or DFS (HR 0,96; CI 95% 0.83 to 1.10; P =0.52 I2 = 36%) when compared to no treatment. No subgroup analysis (immunotherapy, vaccines, biochemotherapy) had relevant results. Toxicity evaluation depicted a significantly higher frequency of serious adverse events in the adjuvant group(OR 73.86; CI 95% 28,32to 192,62; P < 0,00001 I2 = 37%).The result of the analysis provided no support for the hypothesis that the agents studied provide any clinical benefit for renal cancer patients in the adjuvant setting, in addition to increasing the risk of serious adverse events. Randomized trials are underway to test targeted therapies in adjuvant setting, which might open a new therapeutic frontier for these patients. Until these trials yield results, no adjuvant therapy can be recommended for patients who undergo surgical curative resection for renal cell cancer
Mestrado
Clinica Medica
Mestre em Clinica Medica

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50

Mei, Henrik Eckhard. "Analysen zur differentiellen Plasmazellhomöostase beim Menschen." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2010. http://dx.doi.org/10.18452/16048.

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Das humorale Immungedächtnis wird von reifen Plasmazellen des Knochenmarks vermittelt, welche bei Immunreaktionen aus aktivierten B-Lymphozyten gebildet werden. Dabei sind im Blut Plasmablasten als unmittelbare Vorläufer der Plasmazellen nachweisbar, die von dort aus in das Knochenmark einwandern. Anhand der durchflusszytometrischen Detektion spezifischer Plasmablasten gelang es hier, das simultane Auftauchen von Wellen neu generierter, migratorischer Plasmablasten und reifer, nicht-migratorischer Plasmazellen im Blut eine Woche nach einer Tetanusimpfung nachzuweisen. Plasmablasten und Plasmazellen lagen stets im Gleichgewicht vor, wodurch auf die stöchiometrische Mobilisierung reifer Plasmazellen des Knochenmarks durch systemisch induzierte Plasmablasten geschlossen wurde. Ein solcher Verdrängungsmechanismus wird hier erstmalig als Anpassungsmechanismus des humoralen Immungedächtnisses dargestellt, der die Aufnahme neuer Spezifitäten in das Gedächtnis unter Wahrung der Stabilität präexistierender Spezifitäten erlaubt. Anders als systemisch induzierte Plasmablasten, weisen Plasmablasten, die im immunologischen Ruhephase zirkulieren, Kennzeichen mukosaler Immunreaktionen auf: sie exprimieren IgA sowie die mukosalen Zellmigrationsrezeptoren alpha4beta7-Integrin und CCR10. Wahrscheinlich wandern sie in mukosale Plasmazelldepots ein und interferieren nicht mit den Plasmazellen des Knochenmarks, sodass die Stabilität des humoralen Gedächtnisses in der Ruhephase gewahrt bleibt. Eine Anpassung des humoralen Gedächtnisses findet somit nur im Rahmen systemischer Immunreaktionen statt. Bei splenektomierten Patienten und unter der B-Zell-Depletionstherapie bei Rheumapatienten bleiben mukosale Plasmablasten im Blut nachweisbar. Dies belegt deren autonome Bildung aus mukosalen, therapie-refraktären B-Zellen. Insgesamt wird hier eine bisher unbeachtete Komplexität menschlicher peripherer Plasmablasten und Plasmazellen und ihren Beziehungen zum humoralen Immungedächtnis dargestellt.
Humoral memory, i.e. persistence of specific antibody titers, is provided by plasma cells in the bone marrow, which are generated from activated B cells during immune responses. At this, immediate plasma cell precursors, the plasmablasts, migrate via the blood to the bone marrow. Using cytometric detection of antigen-specific plasmablasts, synchronous circulation of waves of recently generated, migratory plasmablasts and non migratory plasma cells with a mature phenotype was demonstrated one week after tetanus vaccination. Circulating plasmablast and plasma cell numbers were always in homeostasis, so that the stoichiometric mobilization of old bone marrow plasma cells by recently generated plasmablasts was hypothesized. This plasma cell replacement mechanism is herein described for the first time as an adaption mechanism of the humoral memory that allows incorporation of new antibody specificities while maintaining pre-existing ones. In immunological steady state, very low numbers of plasmablasts are detectable in any donor. These express IgA and receptors for mucosal homing, alpha4beta7 integrin and CCR10, and therefore most likely migrate into mucosal plasma cell depots and do not interfere with plasma cells of the bone marrow, preserving the stability of humoral memory during steady state. Hence, adaption of humoral memory is only possible during systemic immune reactions. Circulating mucosal plasmablasts produced during steady state remain detectable in patients with rheumatoid arthritis during B cell depletion therapy as well as in asplenic patients. Hence, this type of plasmablasts is self-sufficiently generated from mucosal B cells that are refractory to B cell depletion therapy. This work demonstrates a hitherto disregarded complexity of peripheral plasmablast and plasma cell subsets in healthy humans, with implications for the regulation of induction and maintenance of humoral memory.

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Dissertations / Theses on the topic 'Immunological Analyses'

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