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Journal articles on the topic 'Immunohistochemistry and histology'

Author: Grafiati

Published: 9 March 2023

Last updated: 10 March 2023

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1

Renshaw, Andrew A., and Christopher L. Corless. "Histology and Immunohistochemistry." American Journal of Surgical Pathology 19, no. 7 (July 1995): 842–49. http://dx.doi.org/10.1097/00000478-199507000-00013.

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2

Mazroa, Shireen A. "Immunohistochemistry." Egyptian Journal of Histology 35, no. 2 (June 2012): 191–97. http://dx.doi.org/10.1097/01.ehx.0000414291.44156.ef.

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3

Tzannatou, C. "Histology and immunohistochemistry of placental tissues." Journal of Reproductive Immunology 81, no. 2 (September 2009): 136. http://dx.doi.org/10.1016/j.jri.2009.06.177.

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4

Dhom, G., G. Seitz, and N. Wernert. "Histology and Immunohistochemistry Studies in Prostate Cancer." American Journal of Clinical Oncology 11 (1988): S37–42. http://dx.doi.org/10.1097/00000421-198801102-00009.

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5

Rousselle, Serge D., Joan R. Wicks, Brian C. Tabb, Armando Tellez, and Maureen O’Brien. "Histology Strategies for Medical Implants and Interventional Device Studies." Toxicologic Pathology 47, no. 3 (February 14, 2019): 235–49. http://dx.doi.org/10.1177/0192623319827288.

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Histology of medical devices poses a variety of unique challenges. Comprehensive histologic assessment of medical devices often requires spatial context and high-quality retention of the device–tissue interface. However, the composition of many medical devices is often not amenable to traditional paraffin embedding and thus alternative specialized methodologies such as hard resin embedding must be used. Hard resin embedding requires specialized laboratory technical expertise and equipment, and the fixation techniques and resin composition used markedly impact the feasibility of immunohistochemistry. For the continuity of spatial context during histologic evaluation, additional imaging methods such as macrophotography, radiography, micro-Computerized Tomography (microCT), or magnetic resonance imaging (MRI) can be used to guide sectioning and to complement histologic findings. Although standardized approaches are scarce for medical devices, important considerations specific to medical device histology are discussed, including general specimen preparation, special considerations for devices by organ system, and the challenges of immunohistochemistry. Histologic preparation of medical devices must be thoughtful, thorough, and tailored to achieve optimal histologic outcomes for complex, valuable, and often limited implant specimens.

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6

Hewitt, Stephen M., Denis G. Baskin, Charles W. Frevert, William L. Stahl, and Eduardo Rosa-Molinar. "Controls for Immunohistochemistry." Journal of Histochemistry & Cytochemistry 62, no. 10 (July 14, 2014): 693–97. http://dx.doi.org/10.1369/0022155414545224.

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7

Podkletnova, I., and H. Alho. "Ultrasound-amplified immunohistochemistry." Journal of Histochemistry & Cytochemistry 41, no. 1 (January 1993): 51–56. http://dx.doi.org/10.1177/41.1.8417112.

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We describe a novel technique for improving the sensitivity of immunofluorescence staining by use of ultrasonic irradiation. Free-floating vibratome sections from rat cerebellum were incubated with primary antiserum and simultaneously were briefly exposed to ultrasound (US) in a conventional ultrasound bath. After the US treatment, a conventional immunohistochemical method was employed. Two different antisera and two conventional immunohistochemical detection systems were tested. In all cases a 10-20-sec US treatment strengthened immunoreactivity considerably. Irradiated samples showed a good morphology compared with non-irradiated sections. Our results demonstrate that with ultrasonic treatment the dilutions of primary antibodies can be increased and the incubation times of primary antisera can be reduced. The ultrasonic method described here requires no special equipment. It is easy, reproducible, and it can be considered a new method for the enhancement at immunohisto- and cytochemical staining of free-floating vibratome sections.

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8

Fischer, Sandra, and Sylvia L. Asa. "Application of Immunohistochemistry to Thyroid Neoplasms." Archives of Pathology & Laboratory Medicine 132, no. 3 (March 1, 2008): 359–72. http://dx.doi.org/10.5858/2008-132-359-aoittn.

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AbstractContext.—Thyroid lesions with nodular architecture and follicular pattern of growth often pose difficulties in accurate diagnosis during the assessment of cytologic and histologic specimens. The diagnosis of follicular neoplasm on cytology or of follicular tumor of uncertain malignant potential on histology is likely to cause confusion among clinicians and delay effective management of these lesions. Occasionally, thyroid tumors represent unusual or metastatic lesions and their accurate diagnosis requires immunohistochemical confirmation.Objective.—To review the literature on the applications of immunohistochemistry in the differential diagnosis of thyroid tumors.Data Sources.—Relevant articles indexed in PubMed (National Library of Medicine) between 1976 and 2006.Conclusions.—Our review supports the use of ancillary techniques involving a panel of antibodies suitable for immunohistochemistry and molecular analysis in the assessment of thyroid nodules. These tools can improve diagnostic accuracy when combined with standard morphologic criteria.

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9

Lamperti, Costanza, and Maurizio Moggio. "Muscular Dystrophies: Histology, Immunohistochemistry, Molecular Genetics and Management." Current Pharmaceutical Design 999, no. 999 (March 10, 2010): 1–10. http://dx.doi.org/10.2174/1381210200418286128.

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10

Hendrick, M. J., and J. J. Brooks. "Postvaccinal Sarcomas in the Cat: Histology and Immunohistochemistry." Veterinary Pathology 31, no. 1 (January 1994): 126–29. http://dx.doi.org/10.1177/030098589403100121.

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11

WELLS, M., and J. N. BULMER. "The human placental bed: histology, immunohistochemistry and pathology." Histopathology 13, no. 5 (April 3, 2007): 483–98. http://dx.doi.org/10.1111/j.1365-2559.1988.tb02073.x.

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12

MAYALL, F. G., and A. R. GIBBS. "The histology and immunohistochemistry of small cell mesothelioma." Histopathology 20, no. 1 (January 1992): 47–51. http://dx.doi.org/10.1111/j.1365-2559.1992.tb00915.x.

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13

Lamperti, Costanza, and Maurizio Moggio. "Muscular Dystrophies: Histology, Immunohistochemistry, Molecular Genetics and Management." Current Pharmaceutical Design 16, no. 8 (March 1, 2010): 978–87. http://dx.doi.org/10.2174/138161210790883471.

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14

Grube, Dietrich. "Constants and variables in immunohistochemistry." Archives of Histology and Cytology 67, no. 2 (2004): 115–34. http://dx.doi.org/10.1679/aohc.67.115.

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15

Fritz, Peter, Hans-Volker Tuczek, Hinke Multhaupt, Joachim Hoenes, Dagmar Lutz, Rainer Doerrer, and Peter Schwarzmann. "Quantitative Immunohistochemistry." Progress in Histochemistry and Cytochemistry 24, no. 3 (January 1992): III—53. http://dx.doi.org/10.1016/s0079-6336(11)80214-7.

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16

Tan, Geok Chin. "Molecular Subtyping of Paediatric Medulloblastoma by Immunohistochemistry." Medicine & Health 15, no. 2 (December 31, 2020): 124–39. http://dx.doi.org/10.17576/mh.2020.1502.13.

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Four core molecular subgroups of medulloblastomas have recently been introduced disparate by their transcriptional profile, all of which have different prognostic value. We aimed to determine the histological variants and molecular subtypes of medulloblastomas by immunohistochemistry in our population, and to correlate that with clinicopathological parameters. Seventeen patients aged four-month to 14.3 years diagnosed with medulloblastoma were recruited from year 2002 to 2017. All medulloblastomas were assigned to various histological variants and molecular subgroups by immunohistochemistry surrogate markers (YAP-1 and beta catenin). They were then correlated with clinicopathological parameters and outcomes. Classic histology (76.5%) was the commonest, followed by large cell/anaplastic (LCA) (17.6%) and desmoplastic/nodular (DN) variants (59%). The most frequent molecular subgroup was non-SHH/WNT tumours (64.7%), seconded by SHH tumours (35.3%). Among the SHH tumours, 66.7% was classic histology and the remaining 33.3% was LCA variant. Interestingly, one DN histology demonstrated YAP-1 and beta catenin immunonegativity, denoting non-SHH/WNT molecular subgroup. Majority (88.2%) medulloblastomas were at midline 4th ventricle location, including DN variant. Estimated three-year diseasefree- survival (DFS) and overall-survival (OS) was 60% and 86.7%, respectively. Age <3 years at diagnosis, tumour size >5 cm, LCA histology and high risk group were inversely correlated with DFS, with early relapse. Infant <3-year-old had worse OS. Other factors had no significant impact on DFS and OS. We had demonstrated the feasibility of simple immunohistochemistry-based surrogate markers (YAP-1 and beta catenin) to stratify medulloblastomas into distinct molecular subtypes. Prognostic and predictive values of YAP-1 immunomarker in medulloblastomas however await further investigations.

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Munday, J. S., N. L. Stedman, and L. J. Richey. "Histology and Immunohistochemistry of Seven Ferret Vaccination-site Fibrosarcomas." Veterinary Pathology 40, no. 3 (May 2003): 288–93. http://dx.doi.org/10.1354/vp.40-3-288.

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The anatomical location, histology, and immunohistochemistry of 10 ferret dermal and subcutaneous fibrosarcomas were examined. Seven of the 10 tumors were from locations used for vaccination. All fibrosarcomas contained spindle-shaped cells surrounded by variable quantities of connective tissue stroma. However, vaccination-site fibrosarcomas (VSFs) subjectively contained a higher degree of cellular pleomorphism. Multinucleated cells were present in three of seven VSFs but not in any of the nonvaccination- site fibrosarcomas (NVSFs). Large histiocytic cells, interpreted as macrophages, containing intracytoplasmic basophilic granular material were observed in two VSFs but not in any of the NVSFs. Five VSFs contained peripheral lymphoplasmacytic aggregates. Immunohistochemically, three VSFs stained with anti– smooth muscle actin antibodies and one stained with antibodies against desmin. No expression of muscle cytoskeletal filaments was observed in any NVSF. Filaments interpreted as actin were visible in both the VSFs examined ultrastructurally. One of the VSFs examined ultrastructurally contained intracytoplasmic crystalline material. The preferential development of subcutaneous fibrosarcomas in vaccination sites suggests that, as in cats, vaccination may promote local sarcoma development in ferrets. Additionally, some of the histologic, immunohistochemical, and ultrastructural features of these tumors are similar to those reported for feline vaccine-associated sarcomas. To the authors' knowledge, vaccination has not previously been reported to be oncogenic in any species other than cats.

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18

Taylor, Clive R. "Immunohistochemistry." Applied Immunohistochemistry & Molecular Morphology 27, no. 5 (2019): 325–26. http://dx.doi.org/10.1097/pai.0000000000000770.

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19

Debeer, Sabine, Jean-Benoît Le Luduec, Dominique Kaiserlian, Philippe Laurent, Jean-François Nicolas, Bertrand Dubois, and Jean Kanitakis. "Comparative histology and immunohistochemistry of porcine versus human skin." European Journal of Dermatology 23, no. 4 (July 2013): 456–66. http://dx.doi.org/10.1684/ejd.2013.2060.

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20

Horny, Hans-Peter, Karl Sotlar, and Peter Valent. "Diagnostic value of histology and immunohistochemistry in myelodysplastic syndromes." Leukemia Research 31, no. 12 (December 2007): 1609–16. http://dx.doi.org/10.1016/j.leukres.2007.05.010.

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21

Schmidt, Dietmar, Dieter Harms, and Vernon A. Pilon. "Small-Cell Pediatric Tumors: Histology, Immunohistochemistry, and Electron Microscopy." Clinics in Laboratory Medicine 7, no. 1 (March 1987): 63–89. http://dx.doi.org/10.1016/s0272-2712(18)30764-9.

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22

Kumar, P., and J. F. Timoney. "Histology, Immunohistochemistry and Ultrastructure of the Equine Tubal Tonsil." Anatomia, Histologia, Embryologia: Journal of Veterinary Medicine Series C 34, no. 3 (June 2005): 141–48. http://dx.doi.org/10.1111/j.1439-0264.2005.00582.x.

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23

Kumar, P., and J. F. Timoney. "Histology, Immunohistochemistry and Ultrastructure of the Equine Palatine Tonsil." Anatomia, Histologia, Embryologia: Journal of Veterinary Medicine Series C 34, no. 3 (June 2005): 192–98. http://dx.doi.org/10.1111/j.1439-0264.2005.00594.x.

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24

Prichard, Jeffrey W. "Overview of Automated Immunohistochemistry." Archives of Pathology & Laboratory Medicine 138, no. 12 (December 1, 2014): 1578–82. http://dx.doi.org/10.5858/arpa.2014-0083-ra.

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Context The increasing demand for immunohistochemistry for clinical diagnostics, in combination with an ongoing shortage of staff in the histology laboratory, has brought about a need for automation in immunohistochemistry. The current automated staining platforms vary significantly in their design and capabilities. Objective To review how technology has been applied to automating the process of immunohistochemical staining. Data Sources Literature review, vendor interviews, and personal practice experience. Conclusions Each of the commercially available, automated immunohistochemistry platforms has strategic design differences that produce advantages and disadvantages. Understanding those differences can help match the demands of testing volumes, turnaround time, standardization, and labor savings to the appropriate automated instrumentation.

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25

Rudzinski, Erin R. "Histology and Fusion Status in Rhabdomyosarcoma." American Society of Clinical Oncology Educational Book, no. 33 (May 2013): 425–28. http://dx.doi.org/10.14694/edbook_am.2013.33.425.

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The International Classification of Rhabdomyosarcoma (ICR) has provided diagnostic criteria for rhabdomyosarcoma (RMS) and formed the basis of histologic risk stratification since its publication in 1995. However, the recognition of new variants of embryonal rhabdomyosarcoma (ERMS), shifts in the diagnostic criteria of alveolar rhabdomyosarcoma (ARMS), the increasing use of myogenin immunohistochemistry and recognition of the distinct biologic properties associated with fusion status all raised questions about the continued use of this classification system in the diagnosis and treatment of patients with RMS. Recent Children's Oncology Group Soft Tissue Sarcoma Committee analysis of histology and fusion status in the intermediate risk RMS study D9803 refined the histologic criteria of RMS. We validated the new diagnostic criteria against fusion status, allowing prospective examination of the prognostic value of histology compared with fusion status for risk-stratification of patients with RMS. This article summarizes the evolution of and current practices in the histologic and molecular classification of rhabdomyosarcoma.

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26

Stewart, J. A. "Immunohistochemistry II." Histopathology 25, no. 3 (September 1994): 294–95. http://dx.doi.org/10.1111/j.1365-2559.1994.tb01337.x.

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27

Everbroeck, Bart Van, Philippe Pals, Jean-Jacques Martin, and Patrick Cras. "Antigen Retrieval in Prion Protein Immunohistochemistry." Journal of Histochemistry & Cytochemistry 47, no. 11 (November 1999): 1465–70. http://dx.doi.org/10.1177/002215549904701112.

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28

Montero, Claudio. "The Antigen-Antibody Reaction in Immunohistochemistry." Journal of Histochemistry & Cytochemistry 51, no. 1 (January 2003): 1–4. http://dx.doi.org/10.1177/002215540305100101.

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The problems of major concern in immunohistochemical practice are discussed in the following order: (a) the mechanism of the Ag-Ab reaction in fixed tissue as opposed to the in vitro reaction; (b) the chemistry of fixation and its influence on the final result of the immunohistochemical reaction; (c) the various procedures used for antigen retrieval in formaldehyde-fixed tissue; and (d) the consideration of the possible mechanism underlying heat-induced antigen retrieval. Suggestions for further work to attempt a clarification of the mechanism involved in the Ag-Ab reaction in immunohistochemistry resorting to existing histochemical methods for the demonstration of protein side groups are presented, together with some examples already published.

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29

Wasielewski, Reinhard von, Michael Mengel, Suzanne Gignac, Ludwig Wilkens, Martin Werner, and Axel Georgii. "Tyramine Amplification Technique in Routine Immunohistochemistry." Journal of Histochemistry & Cytochemistry 45, no. 11 (November 1997): 1455–59. http://dx.doi.org/10.1177/002215549704501102.

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Signal amplification in immunohistochemistry via binding of biotinylated tyramine to proteins near the site of peroxidase-labeled antibodies is a promising new technique, but studies investigating a wide range of markers are lacking. The tyramine amplification technique (TAT) was investigated on 85 antibodies using a simple and fast protocol, and TAT results were compared to those obtained with conventional immunohistochemistry. Using TAT, most of the markers could be 5- to 50-fold further diluted and still showed identical staining results compared with standard stainings (maximal 500-fold). However, the variable reactivity of the different markers with TAT underlines the need for individual testing of every antibody to determine the optimal dilution. Some antibodies against cell adhesion molecules could be demonstrated for the first time in archival, formalin-fixed tissue sections. TAT, if carefully evaluated, offers a revolutionary improvement for modern immunostaining, either to increase sensitivity or primary antibody dilutions (cost reduction). From a methodological point of view, immunohistochemistry has not reached its limits by far and TAT is an important progressive step in this developmental process. (J Histochem Cytochem 45:1455–1459, 1997)

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30

Martinez, Antonio E., Li Lin, and Cherie H. Dunphy. "Grading of Follicular Lymphoma: Comparison of Routine Histology With Immunohistochemistry." Archives of Pathology & Laboratory Medicine 131, no. 7 (July 1, 2007): 1084–88. http://dx.doi.org/10.5858/2007-131-1084-goflco.

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Abstract Context.—Follicular lymphoma (FL) grading is based on the average number of large transformed cells in 10 neoplastic follicles at ×40 high-power field (×10–40 high-power field) examination (grade 1, 0–5 centroblasts per high-power field; grade 2, 6–15 centroblasts per high-power field; grade 3, >15 centroblasts per high-power field). Objective.—Since there may be significant interobserver variability, we analyzed the usefulness of immunohistochemical stains in grading FLs more reliably. Design.—Forty-three FLs initially graded by World Health Organization criteria (grade 1, 12; grade 2, 18; grade 3, 13) were reviewed and stained with CD3, CD20, Ki-67, CD30, CD68, PAX-5, and BCL-6. Retrospective review was performed for the average number of large cells, of large lymphoid cells, of large cells staining with CD3, CD20, BCL-6 (40 cases), and PAX-5, and of all cells staining with CD68, Ki-67, and CD30. Results.—By histologic review, 8 of 43 FLs had a significant grade change (4 cases upgraded and 4 cases downgraded). CD3 and CD30 stained only 0 to 3 large cells and 0 to 3 cells, respectively, in neoplastic follicles. CD68+ cells represented the large nonlymphoid cells. Increasing FL grades demonstrated increases in Ki-67+ cells. The original grade showed substantial agreement with CD20 and moderate agreement with PAX-5 and BCL-6. The original histologic grade agreed with immunohistochemical-based grade using 2 or more antibodies in 5 of 8 discordant cases (4 by CD20 or BCL-6 and PAX-5; 1 by CD20, PAX-5, and BCL-6). Conclusions.—Interobserver variability of histologic FL grading may be significant; we showed low-end “substantial agreement.” Immunohistochemical stains (ie, CD20, PAX-5, and BCL-6) may more reliably determine the number of large transformed cells in neoplastic follicles; Ki-67 staining correlates with higher FL grades. Immunohistochemical stains may be evaluated in clinical trials of FL patients to determine prognostic significance.

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31

Juhlin, C. Christofer. "Challenges in Paragangliomas and Pheochromocytomas: from Histology to Molecular Immunohistochemistry." Endocrine Pathology 32, no. 2 (March 25, 2021): 228–44. http://dx.doi.org/10.1007/s12022-021-09675-0.

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AbstractAbdominal paragangliomas and pheochromocytomas (PPGLs) are rare neuroendocrine tumors of the infradiaphragmatic paraganglia and adrenal medulla, respectively. Although few pathologists outside of endocrine tertiary centers will ever diagnose such a lesion, the tumors are well known through the medical community—possible due to a combination of the sheer rarity, their often-spectacular presentation due to excess catecholamine secretion as well as their unrivaled coupling to constitutional susceptibility gene mutations and hereditary syndromes. All PPGLs are thought to harbor malignant potential, and therefore pose several challenges to the practicing pathologist. Specifically, a responsible diagnostician should recognize both the capacity and limitations of histological, immunohistochemical, and molecular algorithms to pinpoint high risk for future metastatic disease. This focused review aims to provide the surgical pathologist with a condensed update regarding the current strategies available in order to deliver an accurate prognostication of these enigmatic lesions.

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32

Yuan, Shi-Min, Yan-Qing Wang, Yi Shen, and Hua Jing. "Transforming growth factor-β in graft vessels: histology and immunohistochemistry." Clinics 66, no. 5 (2011): 895–901. http://dx.doi.org/10.1590/s1807-59322011000500029.

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33

Arias-Stella, Javier, Philip H. Lieberman, Robert A. Erlandson, and Javier Arias-Stella. "Histology, Immunohistochemistry, and Ultrastructure of the Verruga in Carrionʼs Disease." American Journal of Surgical Pathology 10, no. 9 (September 1986): 595–610. http://dx.doi.org/10.1097/00000478-198609000-00002.

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34

Chessa, Daniela, Paola Delaconi, Nikki Kelvin, Franco Campus, Luca Sanna, Maria A. Demurtas, David Kelvin, Salvatore Rubino, and Vittorio Mazzarello. "Fluorescent Immunohistochemistry." Applied Immunohistochemistry & Molecular Morphology 25, no. 4 (April 2017): 289–97. http://dx.doi.org/10.1097/pai.0000000000000302.

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35

Handra-Luca, Adriana. "ERG1 Immunohistochemistry." Applied Immunohistochemistry & Molecular Morphology 27, no. 2 (February 2019): e20-e21. http://dx.doi.org/10.1097/pai.0000000000000578.

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36

GRUBE, Dietrich, and Yoshisuke KUSUMOTO. "Serial Semithin Sections in Immunohistochemistry: Techniques and Applications." Archives of Histology and Cytology 49, no. 4 (1986): 391–410. http://dx.doi.org/10.1679/aohc.49.391.

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37

Eisen, Richard N. "Quality management in immunohistochemistry." Diagnostic Histopathology 14, no. 7 (July 2008): 299–307. http://dx.doi.org/10.1016/j.mpdhp.2008.06.003.

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38

Hisano, S., T. Adachi, and S. Daikoku. "Freeze-drying technique in electron microscopic immunohistochemistry." Journal of Histochemistry & Cytochemistry 33, no. 5 (May 1985): 485–90. http://dx.doi.org/10.1177/33.5.3989275.

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Postembedding immunocytochemical labeling was performed on sections of rat neurohypophysis prepared by either freeze-drying, vapor fixation and Spurr resin embedding, or conventional aqueous fixation and Spurr resin embedding. Arginine vasopressin (AVP) and oxytocin (OXT) were immunolabeled with protein A-gold-anti-AVP and protein A-gold-anti-OXT complexes, respectively. The freeze-drying procedure (FD) resulted in excellent preservation of ultrastructure and greater antigenicity than the conventional procedure (Con). More gold particles were seen over secretory granules in FD sections than in Con sections. In addition, in FD sections, the gold label was restricted to secretory granules while in Con sections, both the granules and the extragranular axoplasm exhibited label. The two antigens in FD sections could be labeled simultaneously with protein A-small gold particle-anti-OXT complex and protein A-large gold particles-anti-AVP complex. In this way the two antigens were seen to be present in secretory granules within different axon terminals. Thus FD preparations should be useful for demonstrating the presence of multiple antigens in the same granules of nerve terminals.

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39

Shi, Shan-Rong, Richard J. Cote, and Clive R. Taylor. "Antigen Retrieval Immunohistochemistry: Past, Present, and Future." Journal of Histochemistry & Cytochemistry 45, no. 3 (March 1997): 327–43. http://dx.doi.org/10.1177/002215549704500301.

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The antigen retrieval (AR) technique, which is predominantly based on high-temperature heating of tissues, is used as a non-enzymatic pretreatment for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. It has been widely applied in pathology and analytical morphology. The existence of a growing body of literature on the AR technique raises a number of interesting issues for the further development of AR. These issues include the use of a “test battery” and the concept of “maximal retrieval” applied to the selection of optimal test protocols for the standardization of AR. (J Histochem Cytochem 45:327–343, 1997)

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40

Alvaro, T., M. Gomez-Morales, D. Aguilar, and J. Aneiros. "Immunohistochemistry of meningiomas." Histopathology 15, no. 3 (September 1989): 316–17. http://dx.doi.org/10.1111/j.1365-2559.1989.tb03092.x.

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41

Romagnoli, S., P. Braidotti, F. Di Nuovo, and G. Coggi. "Amyloid tumour (amyloidoma) of the leg: histology, immunohistochemistry and electron microscopy." Histopathology 35, no. 2 (August 1999): 188–89. http://dx.doi.org/10.1046/j.1365-2559.1999.0744f.x.

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42

GOING, J. J., A. B. LUMSDEN, and T. J. ANDERSON. "A classical osteogenic sarcoma of the breast: histology, immunohistochemistry and ultrastructure." Histopathology 10, no. 6 (June 1986): 631–41. http://dx.doi.org/10.1111/j.1365-2559.1986.tb02516.x.

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43

Shirai, Kumi, Shizuya Saika, Yuka Okada, Seiji Oda, and Yoshitaka Ohnishi. "Histology and immunohistochemistry of fibrous posterior capsule opacification in an infant." Journal of Cataract & Refractive Surgery 30, no. 2 (February 2004): 523–26. http://dx.doi.org/10.1016/s0886-3350(03)00616-3.

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44

Kaschke, Oliver, Kathrin Woelke, Guenter Jautzke, and Bernd Seefeld. "Classification of Etiology Agents in Fungal Sinusitis by Immunohistochemistry and Histology." Otolaryngology–Head and Neck Surgery 131, no. 2 (August 2004): P302. http://dx.doi.org/10.1016/j.otohns.2004.06.667.

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45

Patrizio, P., J. Bromer, J. Johnson, M. Martel, S. Silber, and A. Arav. "Cryopreservation of eleven whole human ovaries: histology, immunohistochemistry and technical details." Fertility and Sterility 90 (September 2008): S38. http://dx.doi.org/10.1016/j.fertnstert.2008.07.620.

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46

Kobayashi, Yasunao, Misako Nakashima, Takashi Wakakuri, Junko Imaki, and Masataka Ito. "Histology and immunohistochemistry of the developing juxta-oral organ in mice." Annals of Anatomy - Anatomischer Anzeiger 198 (March 2015): 49–57. http://dx.doi.org/10.1016/j.aanat.2014.11.004.

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47

Pucci, Angela, Cristina Zanini, Federica Ferrero, Riccardo Arisio, Andrea Valori, Piero Abbruzzese, and Marco Forni. "Misalignment of lung vessels: diagnostic role of conventional histology and immunohistochemistry." Virchows Archiv 442, no. 6 (May 13, 2003): 597–600. http://dx.doi.org/10.1007/s00428-003-0814-2.

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48

Talaulikar, Dipti, Jane Esther Dahlstrom, Bruce Shadbolt, Amy Broomfield, and Anne McDonald. "Role of Immunohistochemistry in Staging Diffuse Large B-cell Lymphoma (DLBCL)." Journal of Histochemistry & Cytochemistry 56, no. 10 (June 23, 2008): 893–900. http://dx.doi.org/10.1369/jhc.2008.951087.

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The use of immunohistochemistry (IHC) in staging bone marrow in non-Hodgkin's lymphoma (NHL) is largely limited to ambiguous cases, particularly those with lymphoid aggregates. Its role in routine clinical practice remains unestablished. This study aimed to determine whether the routine use of IHC in diffuse large B-cell lymphoma (DLBCL) would improve the detection of lymphomatous involvement in the bone marrow. It also sought to determine the impact of IHC on predicting survival compared with routine histological diagnosis using hematoxylin and eosin (H&E), Giemsa, and reticulin staining. The bone marrow trephines of 156 histologically proven DLBCL cases were assessed on routine histology, and IHC using two T-cell markers (CD45RO and CD3), two B-cell markers (CD20 and CD79a), and κ and λ light chains. IHC detected lymphomatous involvement on an additional 11% cases compared with histology alone. Although both routine histology and IHC were good predictors of survival, IHC was better at predicting survival on stepwise multivariate Cox regression analysis. IHC performed routinely on bone marrow trephines has the ability to improve detection of occult lymphoma in experienced hands. Furthermore, it is a better predictor of survival compared with routine histological examination alone.

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O'Leary, Timothy J. "Standardization in Immunohistochemistry." Applied Immunohistochemistry & Molecular Morphology 9, no. 1 (March 2001): 3–8. http://dx.doi.org/10.1097/00129039-200103000-00002.

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Sramek, Brett, Allison Lisle, and Timothy Loy. "Immunohistochemistry in ocular carcinomas." Journal of Cutaneous Pathology 35, no. 7 (July 2008): 641–46. http://dx.doi.org/10.1111/j.1600-0560.2007.00871.x.

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