Dissertations / Theses on the topic 'Immunoglobulin A; Immunoglobulin G; Antigens'
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Hirano, Ayumi. "T dependent B cell help in cattle : immunoregulatory function of interleukin-4 and CD40-CD40L interactions /." free to MU campus, to others for purchase, 1997. http://wwwlib.umi.com/cr/mo/fullcit?p9841150.
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Carson, Richard Thomas. "Immunoglobulin G subclass responses to bacterial protein antigens in man." Thesis, University of Newcastle Upon Tyne, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384823.
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Bartholomeuz, Risien Chiron Andrew. "Polymeric IgA antibody in humans after vaccination and in disease /." Title page, contents and abstract only, 1989. http://web4.library.adelaide.edu.au/theses/09MD/09mdb287.pdf.
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Lin, Shiming. "Carboxyl-terminal cysteinylation of immunoglobulin G for orientated immobilisation." Thesis, University of Cambridge, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.388509.
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Hollén, Elisabet. "Coeliac disease in childhood : on the intestinal mucosa and the use of oats /." Linköping : Univ, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-7690.
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Israelsson, Elisabeth. "Host genetic factors and antibody responses with potential involvement in the susceptibility to malaria." Doctoral thesis, Stockholm : Department of Immunology, the Wenner-Gren Institute, Stockholm university, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-8301.
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Lee, Katherine Shi-Hui. "The host immune response to Streptococcus pneumoniae : bridging innate and adaptive immunity /." Download the dissertation in PDF, 2006. http://www.lrc.usuhs.mil/dissertations/pdf/lee2006.pdf.
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Chong, Wai-po, and 莊偉波. "Tolerogenic and inflammatory properties of natural killer cells after interacting with apoptotic cells and immunoglobulin G opsonizedapoptotic cells." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2007. http://hub.hku.hk/bib/B40203633.
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Chong, Wai-po. "Tolerogenic and inflammatory properties of natural killer cells after interacting with apoptotic cells and immunoglobulin G opsonized apoptotic cells." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/hkuto/record/b40203633.
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Maier, Shannon Marie. "Murine models in the investigation of lupus etiology." Oklahoma City : [s.n.], 2006.
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Rousserie, Gilles. "Développement de bionansondes en biofonctionnalisant des boîtes quantiques (quantum dots) par des anticorps." Thesis, Reims, 2012. http://www.theses.fr/2012REIMS040/document.
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Ce travail porte sur différentes méthodes de détection de protéines par des anticorps (Acs) marqués par des boîtes quantiques (QD, « quantum dots »). La première partie de la thèse porte sur le développement et l'optimisation de méthodes de conjugaison d'Acs polyclonaux à des QDs. Une étude de 2007 avait démontré que les conjugués anticorps-quantum dots (Acs-QDs) commerciaux ne présentent que de très rares anticorps fonctionnels à leur surface [Pathak et al., 2007]. Nous avons donc : (i) développé des conditions de réduction ménagée des ponts disulfures des Acs en utilisant soit du dithiothreitol, soit du 2-mercapotethanolamine, (ii) purifié les fragments d'Acs fonctionnels grâce à une colonne d'affinité, (iii) conjugué ces fragments fonctionnels d'Acs aux QDs en utilisant l'agent de liaison amine-thiol SMCC et (iv) développé une méthode de purification des conjugués sur gel d'agarose afin d'éliminer les fragments d'Acs non conjugués et les QDs libres. Des tests en cytométrie en flux ont permis de déterminer l'efficacité des conjugués pour détecter l'expression de la molécule CD4 à la surface de lymphocytes T humains. Un marquage de CD4 réalisé avec des conjugués préparés selon notre méthode s'avère être cinq fois plus sensible qu'en utilisant des conjugués réalisés selon les recommandations commerciales. Une autre méthode de préparation des Acs pour la conjugaison aux QDs a été testée : l'ajout de groupements fonctionnels sulfhydriles sur des amines primaires grâce au N-succinimidyl S-acetylthioacetate (SATA). Cependant, cette méthode ne permet pas d'avoir un contrôle précis du nombre ni de l'emplacement des groupements fonctionnels sulfhydriles ainsi ajoutés. Les tests de conjugaison aux QDs qui ont été réalisés avec ces Acs-SH, en utilisant l'agent de liaison SMCC, a entraîné la formation d'agrégats de taille variable. Cette méthode a donc été abandonnée.La seconde partie de la thèse porte sur la démonstration de la faisabilité et l'optimisation d'un marquage de l'antigène carcino-embryonnaire (CEA, « carcinoembryonic antigen ») humain à la surface de lignées cellulaires murines avec un anticorps simple domaine (sdAb) anti-CEA biotinylé détecté grâce à des conjugués streptavidine-QDs commerciaux et analysé par cytométrie en flux. Ces sdAbs ont été biotinylés selon deux approches : (i) avec des agents de biotinylation chimique qui permettent d'ajouter une biotine sur les amines primaires (biotinylation in vitro) et (ii) par une enzyme lors de leur production par E. coli (biotinylation in vivo). La détection du CEA humain à la surface des 100 000 cellules (lignées cellulaires murine MC38 et MC38-CEA) par ces sdAbs biotinylés a été testée en cytométrie de flux puis comparée à celle obtenue par un anticorps monoclonal biotinylé in vitro. Les résultats ont démontré que les sdAbs biotinylés ont une sensibilité de détection similaire que la biotinylation soit réalisée in vitro ou in vivo. Par contre, la sensibilité de la détection du CEA est environ cinquante fois meilleure en utilisant des sdAbs biotinylés (0,6 fmol d'anticorps sont nécessaires pour détecter le CEA) qu'avec des anticorps monoclonaux biotinylés (33 fmol)We have been working on different ways to detect proteins with antibodies (Abs) labeled by quantum dots (QDs). The first part of his work is to develop and optimize the conjugation of polyclonal antibodies to quantum dot. A study has reported that commercial antibody-quantum dots conjugates (Abs-QDs) present very few functional Ab fragments at the surface of the conjugate [Pathak et al., 2007]. We had: (i) developed an advanced procedure of antibody reduction using dithiothreitol (DTT) or 2-mercaptoethanolamine (2-MEA) (to prevent the loss of antibody functions), (ii) purified the active fragments by affinity purification on column, (iii) conjugated the active reduced antibody fragments to QDs using the amine-thiol crosslinker SMCC for SH coupling, and (iv) developed the purification of the conjugates on agarose gel to remove free QDs and unconjugated antibody fragments. Our conjugates present about a five times better ability to detect CD4 by flow cytometry on 500 000 isolated human lymphocyte T cells than those made after the commercial procedure. Another procedure for antibody preparation was addition of sulfhydryl groups on primary amines using N-succinimidyl S-acetylthioacetate (SATA). However this procedure presents some variable yield and the number and the localization of sulfhydryl groups added on antibody cannot be fully controlled. Thereby, the conjugation of these Abs-SH to QDs using SMCC chemistry leads to the creation of aggregates. This Ab preparation in preparation for conjugation to QDs was abandoned.The second part of our work focusses on testing and comparing the ability to detect carcinoembryonic antigen (CEA) by flow cytometry with anti-CEA single domain antibodies (sdAbs) labeled by QDs. The sdAbs were biotinylated by using two methods: (i) in vitro chemical biotinylation reagent which adds biotins on primary amines, and (ii) enzymatic in vivo biotinylation during their production in E. coli. These anti-CEA sdAb biotinylation methods were compared to in vitro biotinylated monoclonal Abs against CEA for their respective ability to detect human CEA on 100 000 mice cells (MC38 and MC38-CEA cell lines) using flow cytometry. The results show that the limit detection for biotinylated sdAbs is similar between in vitro and in vivo biotinylation. Furthermore the limit detection with biotinylated sdAb (0.6 fmol are required to detect CEA) is about fifty times better than with biotinylated monoclonal Abs (33 fmol)
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Tachet, des Combes Anne. "Etude de la fixation de CD16 soluble sue les plasmocytes malins : optimisation des conditions expérimentales." Paris 5, 1995. http://www.theses.fr/1995PA05P164.
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Sukumaran, Muralidharan. "Optical sensors for immunoglobulin G." Thesis, University of Cambridge, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.620564.
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Li, Siquan. "Pulsed electric field processing of functional foods." Connect to this title online, 2003. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1054144961.
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Thesis (Ph. D.)--Ohio State University, 2003.Title from first page of PDF file. Document formatted into pages; contains xix, 207 p.; also includes graphics (some col.) Includes bibliographical references (p. 182-193). Available online via OhioLINK's ETD Center
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Chauvin, Alain. "Réponses immunitaires locale et générale chez le mouton infesté expérimentalement par fasciola hepatica Linné, 1758." Tours, 1994. http://www.theses.fr/1994TOUR3314.
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Delprat, Christine. "Rôle du CD40 et des cytokines dans la régulation de la production des sous-classes d'IgG humaines, in vitro." Lyon 1, 1995. http://www.theses.fr/1995LYO1T298.
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Carrera, J. P., Zulma M. Cucunuba, Karen Neira, Ben Lambert, Yaneth Pitti, Jesus Liscano, Jorge L. Garzon, et al. "Endemic and epidemic human alphavirus infections in eastern Panama: An analysis of population-based cross-sectional surveys." American Society of Tropical Medicine and Hygiene, 2020. http://hdl.handle.net/10757/655503.
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Madariaga virus (MADV) has recently been associated with severe human disease in Panama, where the closely related Venezuelan equine encephalitis virus (VEEV) also circulates. In June 2017, a fatal MADV infection was confirmed in a community of Darien Province. We conducted a cross-sectional outbreak investigation with human and mosquito collections in July 2017, where sera were tested for alphavirus antibodies and viral RNA. In addition, by applying a catalytic, force-of-infection (FOI) statistical model to two serosurveys from Darien Province in 2012 and 2017, we investigated whether endemic or epidemic alphavirus transmission occurred historically. In 2017, MADV and VEEV IgM seroprevalences were 1.6% and 4.4%, respectively; IgG antibody prevalences were MADV: 13.2%, VEEV: 16.8%, Una virus (UNAV): 16.0%, and Mayaro virus: 1.1%. Active viral circulation was not detected. Evidence of MADV and UNAV infection was found near households, raising questions about its vectors and enzootic transmission cycles. Insomnia was associated withMADVand VEEV infections, depression symptoms were associated with MADV, and dizziness with VEEV and UNAV. Force-of-infection analyses suggest endemic alphavirus transmission historically, with recent increased human exposure to MADV and VEEV in Aruza and Mercadeo, respectively. The lack of additional neurological cases suggests that severe MADV and VEEV infections occur only rarely. Our results indicate that over the past five decades, alphavirus infections have occurred at low levels in eastern Panama, but that MADV and VEEV infections have recently increased-potentially during the past decade. Endemic infections and outbreaks of MADV and VEEV appear to differ spatially in some locations of eastern Panama.National Institute for Health Research
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Sellers, Lisa K. "Exercise-induced alterations in immunoglobulin (IgA, IgG, IgM) levels in cancer versus non-cancer patients." Muncie, Ind. : Ball State University, 2008. http://cardinalscholar.bsu.edu/384.
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Wiese, Anne Carroll Gordon 1956. "MEDIATORS OF IgE-INDUCED ANAPHYLAXIS: AN IN VITRO STUDY OF MEDIATORS CAUSING CONTRACTION OF RABBIT PULMONARY ARTERY." Thesis, The University of Arizona, 1987. http://hdl.handle.net/10150/291622.
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Rabbits were immunized such that antibodies of the IgE class were preferentially produced. Normal rabbit pulmonary artery sections were challenged with supernatant from antigen-treated sensitized blood cells in the presence of antagonists. The hypothesis is that mediators capable of contracting pulmonary arteries are released from blood cells when blood cells are challenged with antigen. Possible mediators are histamine, serotonin, indomethacin and LTD4. Chlorpheniramine (2.6 x 10⁻⁵M) with methysergide (1 x 10⁻⁵ produced a one-hundred-twenty-fold inhibition of contraction. Chlorpheniramine with FPL-55712 (1 x 10⁻⁵M) did not significantly alter the response seen with chlorpheniramine alone. When supernatant obtained from sensitized blood cells pretreated with 1 x 10⁻⁵ indomethacin was used to challenge muscle rings in the presence of chlorpheniramine the response was also not significantly different from response seen with chlorpheniramine alone. Histamine contracted pulmonary artery. Chlorpheniramine (2.6 x 10⁻⁵ inhibited the response six-hundred-fold. Serotonin also contracted pulmonary artery. Methysergide (10⁻⁵M) blocked the response seven-hundred-fold.
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Rankin, Naomi. "Immunoglobulin G glycoslation as a potential biomarker for multiple sclerosis." Thesis, University of Strathclyde, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.538908.
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Pain, Elisabeth. "Investigation of the immunomodulatory properties of intravenous immunoglobulin G (IVIg)." Thesis, University of Bristol, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.251131.
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Rogers, Stephen. "Glycosylation of immunoglobulin G in cerebrospinal fluid and multiple sclerosis." Thesis, University of Surrey, 2001. http://epubs.surrey.ac.uk/843781/.
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The glycosylation features of CSF oligoclonal IgG, and possible changes in N-glycans of CSF IgG in multiple sclerosis (MS) were studied. After isoelectric focusing (IEF) of CSF, bands were detected using biotinylated lectins and avidin-horseradish peroxidase. Concanavalin A (Con A) binding showed that mannose exists throughout the pH range of oligoclonal IgG. Sambucus nigra antigen (SNA) bound acidic and neutral oligoclonal IgG only, suggesting that alkaline oligoclonal IgG is deficient in sialic acid. Deglycosylation of CSF IgG using peptide-N-glycosidase F suggested that the range of isoelectric points of oligoclonal IgG bands is not due to carbohydrate differences alone. Lectin immunoassays, whereby protein A purified IgG was captured by anti-IgG coated tubes and probed using a range of biotinylated lectins, were used to compare 13 CSF samples from MS patients with 14 control samples. With Con A binding, a significantly higher mean and larger variance was found for the MS group (t-test: P < 0.05). Con A binding correlated with CSF [IgG]/[total protein]% (r=0.390; P=0.0443). Using HPLC to separate oligosaccharides released from IgG by hydrazinolysis and labelled with 2-aminobenzamide, glycans were determined in 7 CSF samples with oligoclonal IgG, and 6 CSF samples without. The ratio of the peak for biantennary fucosylated agalactosyl glycans to total monogalactosylated glycan peaks was lower for the oligoclonal IgG samples (t-test: P=0.0141). The overall results suggested that glycosylation changes occur in CSF IgG in MS, and that oligoclonal IgG contains less sialic acid but more galactose than polyclonal IgG.
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Faustino, Vânia Filipa Martins. "Extraction and purification of immunoglobulin G with aqueous biphasic systems." Master's thesis, Universidade de Aveiro, 2015. http://hdl.handle.net/10773/16144.
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Mestrado em Biotecnologia - Biotecnologia Industrial e AmbientalThe immune system is able to produce antibodies, which have the capacity to recognize and to bind to foreign molecules or pathogenic organisms. Currently, there are a diversity of diseases that can be treated with antibodies, like immunoglobulins G (IgG). Thereby, the development of cost-efficient processes for their extraction and purification is an area of main interest in biotechnology. Aqueous biphasic systems (ABS) have been investigated for this purpose, once they allow the reduction of costs and the number of steps involved in the process, when compared with conventional methods. Nevertheless, typical ABS have not showed to be selective, resulting in low purification factors and yields. In this context, the addition of ionic liquids (ILs) as adjuvants can be a viable and potential alternative to tailor the selectivity of these systems. In this work, ABS composed of polyethylene glycol (PEG) of different molecular weight, and a biodegradable salt (potassium citrate) using ILs as adjuvants (5 wt%), were studied for the extraction and purification of IgG from a rabbit source. Initially, it was tested the extraction time, the effect on the molecular weight of PEG in a buffer solution of K3C6H5O7/C6H8O7 at pH≈7, and the effect of pH (59) on the yield (YIgG) and extraction efficiency (EEIgG%) of IgG. The best results regarding EEIgG% were achieved with a centrifugation step at 1000 rpm, during 10 min, in order to promote the separation of phases followed by 120 min of equilibrium. This procedure was then applied to the remaining experiments. The results obtained in the study of PEGs with different molecular weights, revealed a high affinity of IgG for the PEG-rich phase, and particularly for PEGs of lower molecular weight (EEIgG% of 96 % with PEG 400). On the other hand, the variation of pH in the buffer solution did not show a significant effect on the EEIgG%. Finally, it was evaluated the influence of the addition of different ILs (5% wt) on the IgG extraction in ABS composed of PEG 400 at pH≈7. In these studies, it was possible to obtain EEIgG% of 100% with the ILs composed of the anions [TOS]-, [CH3CO2]-and Cl-, although the obtained YIgG% were lower than 40%. On the other hand, the ILs composed of the anions Br-, as well as of the cation [C10mim]+, although not leading to EEIgG% of 100%, provide an increase in the YIgG%. ABS composed of PEG, a biodegradable organic salt and ILs as adjuvants, revealed to be an alternative and promising method to purify IgG. However, additional studies are still required in order to reduce the loss of IgG.
O sistema imunitário tem como ferramenta mais poderosa a produção de anticorpos, pois estes têm a capacidade de reconhecer e ligar-se a moléculas/organismos patogénicos. Atualmente, existem uma série de doenças que podem ser tratadas com anticorpos, nomeadamente com as imunoglobulinas G (IgG). Assim, o desenvolvimento de processos mais baratos e eficazes para a sua extração e purificação é uma área de interesse em biotecnologia. Os sistemas aquosos bifásicos (SAB) têm sido estudados para o efeito pois permitem a redução de custos e do número de passos envolvidos no processo, em comparação com os métodos convencionais. No entanto, os SAB tradicionais têm-se mostrado pouco seletivos, resultando em baixos fatores de purificação e rendimentos. Neste sentido, a incorporação de líquidos iónicos (LIs) nos SAB pode ser uma alternativa promissora para manipular a seletividade destes sistemas. Neste trabalho, estudaram-se SAB constituídos por polietileno glicol (PEG) de diferentes massas moleculares e um sal biodegradável (citrato de potássio), utilizando LIs como adjuvantes (5% m/m) para a extração de IgG de origem leporídea (coelho). Inicialmente, estudaram-se o tempo de extração, o efeito da variação da massa molecular do PEG em solução tampão de K3C6H5O7/C6H8O7 a pH≈7 e o efeito da variação de pH (5-9) sobre o rendimento (YIgG) e eficiência de extração (EEIgG%) de IgG. Os melhores resultados obtidos em termos de EEIgG% foram conseguidos com uma centrifugação a 1000 rpm durante 10 min para promover a separação de fases e equílibrio seguidos de 120 min de repouso. Este procedimento foi posteriormente aplicado aos restantes ensaios experimentais. Os resultados obtidos no estudo de PEGs de diferentes pesos moleculares revelaram uma elevada afinidade da IgG para a fase de PEG, principalmente com os PEGs de menor peso molecular (96% de EEIgG% com o PEG 400). Por outro lado, a variação de pH da solução tampão não mostrou um efeito significativo sobre a EEIgG%. Por fim, foi avaliada a influência da adição de vários LIs (5% m/m) na extração de IgG nos SABs compostos por PEG 400 a pH≈7. Nestes estudos foi possível obter uma EEIgG% de 100% com os LIs constituídos pelos aniões [TOS]-, [CH3CO2]- e Cl-, apesar dos YIgG% obtidos serem inferiores a 40%. Por outro lado, com LIs constituídos pelos aniões Br-, assim como pelos catião [C10mim]+, não se conseguiram obter valores de 100% para a EEIgG%, mas obtiveram-se melhores resultados em termos de YIgG%. Os SAB constituídos por PEG, um sal orgânico biodegradável e LIs como adjuvantes revelaram-se um método alternativo e promissor para a purificação de IgG. No entanto, são necessários ainda estudos adicionais de modo a reduzir a perda de IgG durante o processo.
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TANAKA, KENJI, SHIMPEI TORII, MICHIO HOMMA, and AYAKO ISHIGURO. "Identification of Candida albicans antigens reactive with immunoglobulin E antibody of human sera." Thesis, American Society for Microbiology, 1992. http://hdl.handle.net/2237/19576.
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Lizeng, Qin. "Dynamics of the HIV-2-specific immunoglobulin A(IgA) response /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-259-4/.
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Murphy, Jonathan Paul. "The cloning, expression, and characterisation of Streptoccal protein G." Thesis, University of Southampton, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.316402.
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Bruns, Nicholas Joseph. "Vitamin A deficiency: Serum cortisol and immunoglobulin G levels in lambs." Thesis, Virginia Tech, 1986. http://hdl.handle.net/10919/45753.
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Serum cortisol and immunoglobulin G (IgG) concentrations were measured to investigate the relationship between vitamin A status and immune function in lambs. Twenty-four crossbred ewe lambs were each fed 900 g·d-1 of a carotene—deficient diet composed of 95.5% whole oats, 3% molasses, .5% trace mineral salt and 1% limestone. All lambs were injected monthly with vitamins D and E and with selenium. The 12 control lambs also received a 100,000 IU oral dose of vitamin A palmitate in capsule form every 2 wk. All lambs were challenged by injecting them with 1 mg ovalbumin in 1 ml of Freund’s complete adjuvant. At the time of challenge, serum vitamin A levels for the control and A-deficient (A—def) lambs were 33.3 and 3.1 ug·dl-1 respectively. Blood was collected prior to and 6, 13, 20 and 34 d post—challenge. The lambs were then reschallenged using the same antigen and blood was obtained 1, 2, 6 and 22 d post—challenge. Lambs were sacrificed at the end of the second challenge period. Spleen weights were obtained and gross post—mortem observations were made at this time.Master of Science
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Hartmann, Susanne Helene. "Ultrastructural evidence of transplacental transport of immunoglobulin-G in the bitch /." [S.l.] : [s.n.], 1997. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.
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Bilal, Jawad, Irbaz B. Riaz, Jennifer L. Hill, and Tirdad T. Zangeneh. "Intravenous Immunoglobulin-Induced Pulmonary Embolism: It Is Time to Act!" LIPPINCOTT WILLIAMS & WILKINS, 2016. http://hdl.handle.net/10150/620829.
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Pulmonary embolism (PE) is a common clinical problem affecting 600,000 patients per year in the United States. Although the diagnosis can be easily confirmed by imaging techniques, such as computed tomographic angiography of the chest, the identification of underlying mechanism leading to PE is important for appropriate duration of anticoagulation, and prevention of subsequent episodes. The differential diagnosis of underlying mechanism is broad and must include careful review of medication history. Drug-related thromboembolic disease can be easily missed and may have catastrophic consequences. The identification of the culprit drug is important for prevention of subsequent episodes and choosing appropriate duration of anticoagulation. We report a case of a middle-aged man who developed PE after administration of intravenous immunoglobulin.
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Davis, Elizabeth Jane 1961. "IDENTIFICATION OF A BOVINE IMMUNOGLOBULIN COMPONENT UNIQUE TO MILK AND COLOSTRUM." Thesis, The University of Arizona, 1986. http://hdl.handle.net/10150/276770.
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Sayed, Rama. "Verifying the analysis of immunoglobulin G subclasses on Siemens Atellica NEPH 630 and evaluating the presence of immunoglobulin deficiency with SARS-CoV2 antibodies." Thesis, Uppsala universitet, Institutionen för medicinsk cellbiologi, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-445446.
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Levels of Immunoglobulin G (IgG)-subclasses are analyzed when patients have reoccurring infections and in order to follow the treatment of IgG4 related disease. The measured quantity of IgG-total can be within the reference interval even if the patient has a deficiency in one of the IgG-subclasses. Due to this Sundsvall’s hospital plans to begin analyzing IgG-subclasses. The aim was to verify the performance level of the analysis IgG-subclasses (IgG1-4) with Siemens Atellica NEPH 630. The method was verified by evaluating the method’s precision and by comparing the sum of IgG-subclasses with the quantity of IgG-total analyzed on Cobas c502. In addition, the reference intervals provided by Siemens were evaluated using samples from blood donors. The study evaluated whether there was a correlation between infection with SARS-CoV2 and a deficiency in IgG-subclasses. The verification began by performing quality control twice daily over a period of four weeks. The IgG-subclasses test was performed on blood donor samples with Siemens Atellica NEPH 630 for evaluation of the reference values. The coefficient of variation was less than 6 % for all four subclasses. The reference values for IgG1, IgG2, IgG3, and IgG4 are all in alliance with the reference values provided by Siemens. The sum of IgG-subclasses corresponded well with IgG-total with a correlation value (R) 0.82. No correlation was found between IgG deficiency and infection with SARS-CoV2. The validation of the analysis of IgG-subclasses was successful with quality measurements within the supplier’s intervals. No adjustments will be needed for the reference intervals.
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Hamawy, Majed Mahmood. "Antigen induced modulation of autonomic nervous system responses in immunoglobulin-E - sensitized rabbit lung." Diss., The University of Arizona, 1988. http://hdl.handle.net/10150/184590.
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The major objective of this project was to examine the potential for mediators of IgE-mediated allergic reactions to alter neural activity. The project was divided into three parts. In Part I, the ability of endogenously released chemical mediators to alter neural activity in vitro was assessed by measuring isometric contractile responses to electrical field stimulation (EFS) (2-128 Hz, 20 V, 0.5 msec. duration) of sensitized rabbit bronchi before and after exposure to the antigen horseradish peroxidase (HRP). Antigen enhanced bronchial responses to EFS with low frequencies: mean log (± S.E.) frequency which produced 20% of maximum response decreased from 1.04 (± 0.05) to 0.90 (± 0.07) Hz (p < 0.05). Responses of unsensitized bronchi were not enhanced by antigen. Chlorpheniramine, an H₁ antagonist, abolished the antigen effect. Antigen did not enhance the responses to exogenous acetylcholine. Hence, the antigen is apparently modulating neural activity and not smooth muscle per se. In Part II, the capacity for histamine to modulate vagally-induced bronchoconstriction in anesthetized, vagotomized, mechanically-ventilated rabbits was examined in vivo. Changes in pulmonary resistance induced by electrically stimulating the cut ends of the vagi (2-32 Hz, 20 V, 0.5 msec. duration) were assessed before and 10 minutes after histamine aerosolization (10 breaths of 10 mg/ml). Histamine inhalation potentiated vagally-induced bronchoconstriction at low frequencies: mean log (± S.E.) frequency producing a 20% change in pulmonary resistance decreased from 0.88 (± 0.09) to 0.56 (± 0.15) (p < 0.05). Chlorpheniramine abolished this effect. In Part III, the dependence on IgE antibodies of the in vitro modulation of neurally-induced contraction of sensitized bronchi was investigated. Rabbits were passively immunized with fractions enriched with HRP-specific IgE, IgG, or IgM antibodies. After 72 hours, rabbits were sacrificed and the responses of bronchi to EFS were assessed before and after antigen challenge. Antigen enhanced the responses to EFS only of bronchi passively sensitized with IgE. Therefore, antigen enhancement of neural activity was dependent on IgE. These studies demonstrate that the interaction between antigen and IgE antibodies can induce the release of chemical mediators which can alter neural activity.
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Dai, Jialu. "Investigation on the stability of freeze dried horseradish peroxidase and immunoglobulin G /." View abstract or full-text, 2010. http://library.ust.hk/cgi/db/thesis.pl?MECH%202010%20DAI.
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Seymour, L. C. W. "The mechanism of transcellular transport of immunoglobulin G in yolk sac tissue." Thesis, Keele University, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355615.
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Blank, Ulrich. "Caracterisation biochimique et purification des immunoglobulin-g-binding factors (igg-bf) murins." Paris 7, 1987. http://www.theses.fr/1987PA077005.
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Les lymphocytes t, porteurs de recepteurs fc, produisent des facteurs de liaison d'immunoglobuline g (igg-bf) qui se lient a la fraction fc des igg et suppriment leur production par les lymphocytes b. L'igg-bf apparait heterogene en poids moleculaire et en charge. Il est n- et o-glycosyli. A l'aide de l'anticorps monoclonal anti-rf::(c)gamma , 2-4g2, il a ete etabli que l'igg-bf porte des sites antigeniques commun avec le rfcgamma , un dosage radioimmunologique a ete developpe sur nitrocellulose. Apres differentes etapes de purification par chromatographie, l'heterogeneite a ete confirmee
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Bland, Ian Mark. "Passive immunity in piglets : the acquisition of immunoglobulin G (IgG) from colostrum." Thesis, University of Aberdeen, 2003. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU602302.
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Foetal piglets do not obtain immunoglobulins during pregnancy and rely on colostrum immunoglobulins until active immunity develops. The amount of immunoglobulin that piglets obtain depends upon: colostrum immunoglobulin concentration; quantities of colostrum ingested and when closure occurs. Closure describes the change that takes place between 12-36h of life, when the piglet gut can no longer absorb immunoglobulins. A series of studies were undertaken to examine colostrum IgG concentrations and IgG acquisition by piglets. The first two trials demonstrated that IgG concentration between sows and piglet IgG intake was very variable. Piglet plasma IgG concentrations were remarkably consistent however, suggested that piglets were regulating IgG uptake. The third trial reduced piglet IgG intakes by delaying suckling. Results showed that piglets with reduced IgG intakes had reduced plasma IgG concentrations and could not compensate for lowered IgG intake. The results suggested that piglets regulated IgG uptake when IgG was in excess, but had only limited ability to regulate IgG uptake when IgG supply was limited. The fourth trial set out to examine the effects of maternal diet and vaccination on piglet IgG acquisition. A 2x2 factorial design, sows were fed diets either with vitamins A, C and E at recommended amounts or 3-5 times recommended amounts and either vaccinated or not. Results showed that maternal vaccination or diet did not affect maternal plasma IgG concentration or colostrum IgG concentration. Supplementing maternal diet with vitamins significantly increased piglet plasma IgG concentrations, as did maternal vaccination. Of ingested IgG, approximately 0.6-0.7 appeared in piglet plasma. It was possible to influence piglet plasma IgG concentrations by manipulating maternal diet and immune status. With increasing pressure on the use of antibiotics in agriculture and increasing animal welfare/health demands, the effects of other macro and micronutrients on piglet IgG status need to be investigated.
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Blank, Ulrich. "Caractérisation biochimique et purification des immunoglobulin G-binding factors (IgG-BF) murins." Grenoble 2 : ANRT, 1987. http://catalogue.bnf.fr/ark:/12148/cb37603067c.
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Li, Xingang. "Heritability enrichment of immunoglobulin G N-glycosylation relevant genes in specific tissues." Thesis, Edith Cowan University, Research Online, Perth, Western Australia, 2020. https://ro.ecu.edu.au/theses/2386.
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Genome-wide association studies (GWAS) have identified over 60 genetic loci associated with IgG N-glycosylation; however, the causal genes and their abundance in relevant tissues are uncertain. In this study, firstly, I leveraged data from GWAS summary statistics for 8,090 Europeans, and large-scale expression quantitative trait loci (eQTL) data from the genotype-tissue expression of 53 types of tissues (GTEx v7), to derive a linkage disequilibrium score for the specific expression of genes (LDSC-SEG) and conduct a transcriptome-wide association study (TWAS). I identified 55 genes whose predicted levels of expression were significantly associated with IgG Nglycosylation in 14 tissues with regard to three working scenarios, i.e., tissue-specific, pleiotropic and co-associated, for candidate genetic predisposition affecting IgG N-glycosylation traits. Secondly, through pathway enrichment, I defined 23 of 55 candidate genes being enriched in several IgG N-glycosylation-related pathways, such as asparagine N-linked glycosylation, Nglycan biosynthesis and transport to the Golgi and subsequent modification. Thirdly, through phenome-wide association studies (PheWAS), I found most genetic variants underlying TWAS hits being correlated with health measures (height, waist-hip ratio, systolic blood pressure) and diseases, such as systemic lupus erythematosus, inflammatory bowel disease and Parkinson’s disease, which are related to IgG N-glycosylation. This study provides an atlas of genetic regulatory loci and their target genes within functionally relevant tissues, for further studies on the mechanisms of IgG N-glycosylation and its related diseases.
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Tomičić, Sara. "Environmental and immunological factors associated with allergic disease in children /." Linköping : Department of Clinical and Experimental Medicine, Linköping University, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-11615.
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Hara, Cristiane de Castro Pernet. "Marcadores da imunomodulação no sangue materno e fetal e nas placentas de mães diabéticas ou com hiperglicemia gestacional leve." Botucatu, 2016. http://hdl.handle.net/11449/148574.
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Orientador: Iracema de Mattos Paranhos CalderonCoorientador: Adenilda Cristina Honório França
Banca: Yuri Karen Sinzato
Banca: Debora Cristina Damasceno Meirelles dos Santos
Banca: Mahmi Fujimori
Banca: Mara Sandra Hoshida
Resumo: Durante a gravidez, a hiperglicemia materna altera a expressão e a transferência de células imunorregulatórias e de imunoglobulinas e o perfil de citocinas na interface materno-fetal. Avaliar, em gestações complicadas por hiperglicemia, a expressão de células NK e o perfil de citocinas no sangue, materno e do cordão umbilical, e na placenta (artigo 1); quantificar a produção de anticorpos e a passagem de IgG total, e respectivas subclasses, via receptor FcRn (artigo 2). MÉTODO - Foram avaliadas 120 gestantes, distribuídas nos grupos: não-diabético (ND; N = 30), hiperglicemia gestacional leve (HGL; N = 30), diabetes mellitus gestacional (DMG; N = 30) e diabetes mellitus tipo 2 (DM2; N = 30). Técnicas de citometria de fluxo foram utilizadas para análise de células e citocinas e, de ELISA, para avaliação das concentrações de IgG total e subclasses. A transferência placentária de anticorpos totais, e respectivas subclasses, foi definida pela relação [(concentrações no sangue de cordão umbilical/sangue materno) x 100]. Na análise estatística foram realizadas análises de variância (ANOVA), seguida pelo teste de Tukey, e de correlação de Pearson, com p < 0,05. No sangue materno dos grupos hiperglicêmicos, as células NK CD16+CD56- aumentaram, enquanto que CD16+CD56+ foi menor no grupo DMG. No sangue do cordão do grupo DM2 mostrou uma maior proporção de células CD16+CD56- e CD16-CD56+. As camadas extravilosas da placenta dos grupos DMG e DM2 most... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: - During pregnancy, the immune response associated with diabetes alters the expression and the transfer of immune cells, including regulatory, immunoglobulins and the profile of cytokines in the maternal-fetal interface. To evaluate the expression of NK cells, and the profile of cytokines in maternal blood, umbilical cord and placenta, quantify the production of antibodies, as well as, the passage of IgG and subclasses, via receptors FcRn in pregnancies complicated by diabetes or hyperglycemia.Wereessed 120 pregnant women, distributed as non-diabetic (ND; n=30), Mild Gestational Hyperglycemia (MGH; n=30), Gestational Diabetes Mellitus (GDM; n=30) and type 2 Diabetes Mellitus (DM2; N=30). The cells and cytokines were evaluated by flow cytometry. The concentrations of total IgG and subclasses were analyzed by ELISA. Placental transfer of the total and subclasses antibodies were defined in each assay by the ratio [(cord concentrations/maternal concentrations) x 100]. In the statistical analysis we used analysis of variance (ANOVA), followed by Tukey test, and Pearson's linear correlation, with p < 0.05. RESULTS - In the maternal blood from the hyperglycemic groups, the CD16+CD56− NK cells increased, whereas that of CD16+CD56+ decreased in GDM group. Cord blood from DM2 showed a higher proportion of CD16+CD56− and CD16−CD56+. The placental extravillous layer of GDM and DM2 showed an increase of CD16+CD56− cells and, irrespective of region, the proportion of CD16−CD56+ cells was higher in MGH and GDM and lower in DM2. IL-2 was lower in maternal blood and IFN-���� higher in maternal and cord blood from the GDM group. IL-17 was higher in maternal and cord blood from the DM-2 group. The placental extravillous layer of the MGH showed high levels of IL-4, IL-6, IL-10, IL- 17, and IFN-���� and low levels of IL-1���� and IL-8, whereas the placental villous layer... (Complete abstract click electronic access below)
Doutor
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Chigerwe, Munashe. "Effect of colostral administration practices on serum immunoglobulin concentration in dairy calves." Diss., Columbia, Mo. : University of Missouri-Columbia, 2008. http://hdl.handle.net/10355/5602.
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Thesis (Ph. D.)--University of Missouri-Columbia, 2008.The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Vita. "May 2008" Includes bibliographical references.
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Phiri, Isaac Khozozo. "Immunoglobulin isotype response of Fasciola spp. infected sheep and cattle to defined Fasciola spp. antigens." Thesis, University of Edinburgh, 1997. http://hdl.handle.net/1842/29943.
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Fasciolosis is a liver fluke disease, caused by Fasciola hepatica in temperate regions and high altitude areas of the tropics and subtropics and by Fasciola gigantica, which is restricted to the tropics and subtropics. Liver flukes have a wide range of definitive hosts, including man and, in particular, domestic ruminants, but the various hosts are known to differ greatly in their resistance to infection with these parasites. For example sheep are considered susceptible to challenge infection while cattle develop resistance. F. hepatica secretes the enzyme cathepsin-L1 protease (Fh-cathepsin) which has a molecular weight (MWt) of 27 kDa. It is considered to have a functional role in parasite evasion of the host immune response, through cleavage of host immunoglobulin. The enzyme, glutathione s-transferase (GST) which is of 27.8-29 kDa MWt, is also secreted by F. hepatica (Fh-GST) and is thought to be involved in the detoxification of exogenous (xenobiotic) and endogenous derived toxic compounds. Both enzymes form part of the fluke excretory/secretory (E/S) products and are of additional interest in that they are considered as vaccine candidates against fasciolosis. This study investigated the immunoglobulin isotype responses of sheep and cattle, chronically infected with F. hepatica and F. gigantica, to defined fluke antigens (F. hepatica E/S products (Fh-E/S) or F. gigantica E/S products (Fg-E/S), Fh-cathepsin and Fh-GST). It was decided to study the immune response in chronically infected animals since immunity is considered to play a potentially more important role in chronic infection than in acute infection, which is characterised by the death of the animal through anaemia and blood loss caused by the migrating flukes. Serum and faecal samples were collected weekly while the severity of the infections were defined using clinical, parasitological, haematological, biochemical and pathological parameters. Serum and faecal antibody (total Ig, IgG1, IgM, IgG2 and IgA) responses to 24-48 hour Fh-E/S, adult Fh-cathepsin and adult Fh-GST were determined by indirect Enzyme-linked Immunosorbent Assay (ELISA). The antigen recognition profile of the Fasciola spp. infected sheep and cattle to Fh-E/S and Fg-E/S was examined by sequential Western blotting.
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Cameron, Angus James MacGregor. "Molecular mechanisms governing Fc#gamma# receptor mediated signal transduction." Thesis, University of Glasgow, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.340327.
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Russell, Alyce Christine. "The N-Glycosylation of immunoglobulin G as a novel biomarker of Parkinson’s disease." Thesis, Edith Cowan University, Research Online, Perth, Western Australia, 2015. https://ro.ecu.edu.au/theses/1617.
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For neurodegenerative diseases, interventions during the early stages of the disease, before significant neurodegeneration has occurred, are associated with an increased probability of slowing or halting the disease process. In order to intervene early, it is essential that an accurate diagnosis is obtained and that disease progression can be monitored. This is particularly relevant for Parkinson’s disease (PD; International Classification of Diseases version 10) because significant neurodegeneration has already occurred by the time the clinical motor symptoms are present. Therefore, the development of translatable, high-throughput biomarkers for large scale population screening is a crucial area of research. Of promise are the emerging “omics” technologies, which enable the detection of preclinical biomolecule fluctuations associated with the development of different diseases.
One such field is glycomics which is the study of the set of sugar structures, hereon in known as glycans, in a given protein, cell or tissue. Notably, the functional diversity of proteins is increased by several magnitudes with the addition of glycans, a process known as glycosylation. The glycosylation of certain proteins, including immunoglobulin G (IgG), has been found to remain fairly stable over short periods of time, with modifications thought to result from changes in the cellular environment or disease presence. Indeed, IgG has the ability to exert both anti-inflammatory and pro-inflammatory effects throughout the body and these properties are controlled by the N-glycosylation of the fragment crystallisable (Fc) portion.
To our knowledge, this was the first time that the potential of using IgG glycomic biomarkers to identify people with PD, as well as identify people with PD who are at risk of cognitive decline, was investigated. It was demonstrated that the peripheral IgG glycome in the PD cases was indicative of an increased capacity to biologically age. While advancing age has previously been associated with modifications to the glycosylation of IgG, making them more pro-inflammatory, advancing age was only associated with significant increases in modifications to the peripheral IgG glycome that infer more pro-inflammatory IgG in the PD cases but not the controls. In PD, the severity of the underlying pathology increases as the individual ages and, therefore, is a confounder of the effect of advancing age on pro-inflammation. Consequently, the peripheral IgG in people with PD have a propensity to become more pro-inflammatory at a faster rate as they age, and this may be linked to the severity of pathology during the course of the disease. PD has a heterogeneous presentation of clinical symptoms, and many factors contribute to the development of the disease. While this is true, it was demonstrated that the peripheral IgG glycome does not have utility in identifying risk of cognitive decline, which would result from progression of PD pathology in the central nervous system (CNS). These results are indicative of the peripheral IgG interacting with PD pathology in the enteric nervous system (ENS) as well as when it propagates from the ENS to the CNS along the vagal nerve. Inflammation may facilitate the neuron-to-neuron propagation of PD inclusions along this pathway and thus be a contributor to PD development during the prodromal phase. Hence, the peripheral IgG glycome may be useful as a novel biomarker of PD presence in the prodromal phase of the disease.
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Plummer, Ben Thomas. "The influence of pH on the binding of immunoglobulin G to staphylococcal protein A." Thesis, University of Canterbury. Chemical and Process Engineering, 2013. http://hdl.handle.net/10092/7952.
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The interaction between protein A and immunoglobulin G (IgG) was studied at a variety of pH values using a surface plasmon resonance (SPR) device, which provides real time kinetic data without labelling or molecular alteration. This study was carried out due to the large scale use of Protein A affinity chromatography for the purification of IgG for pharmaceutical purposes, and is one of the most costly steps in the purification process. The results produced were largely in line with those produced in previous literature with binding remaining strong between pH 7.4 and 5.0, although the association rate decreased as pH decreased. Below pH 5.0, the rate of IgG elution markedly increased, with pH 3.5 showing near full elution seconds after the association phase of the SPR interaction finished. Problems were encountered with non-specific binding between the SPR sensor chip and IgG occurring under a variety of conditions, requiring various remedies. However, no complete interactions were successfully carried out under pH 5.0, so the results obtained below this value were obtained by binding at pH 7.4 and then elution at the desired pH.
The data showed binding behaviour that was most successfully explained by a three-site model, each with a binding ratio of 1:1. The binding ratio is questionable given that Protein A and IgG typically bind at a ratio of 1:2 but may be explained by the sites being independent of one another and thus no secondary attachment is observed. A variety of models were fitted to the data but only two- and three-site models fitted the experimental data, with the three-site model being a more accurate and robust fit across pH changes. A multiple site model seems intuitively correct given the six different binding sites that Protein A has for interaction with IgG. The models produced have potential applications in a larger model of Protein A affinity chromatography, although a number of additional factors would need to be taken into account, such as mass transfer effects and the IgG concentration gradient.
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Yu, Xinwei. "Immunoglobulin G N-glycan profiling as a risk stratification biomarker for type 2 diabetes." Thesis, Edith Cowan University, Research Online, Perth, Western Australia, 2019. https://ro.ecu.edu.au/theses/2199.
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Objective
Type 2 diabetes (T2DM) is a complicated and comprehensive disease that has many metabolic facets with its prevalence increasing with age. The inflammation hypothesis of T2DM was proposed at the American Diabetes Association’s 65th scientific session and subsequently validated in many studies. Immunoglobulin G (IgG) is the main component of antibody formation after a secondary immune response and therefore plays an important role in the inflammation cascade and in the occurrence and development of age-related diseases. N-glycosylation, an important post-translation modifying process, significantly affects the function of IgG molecules, including complement activation, receptor binding properties, molecular recognition and aggregation. As T2DM is a low-grade chronic inflammatory disease and IgG N-glycosylation involves in the inflammation cascade, we hypothesised that IgG N-glycans could be involved in pathophysiological process of T2DM. To provide clues to the molecular mechanisms, and screen intervention targets and search for early diagnosis marker of T2DM, we conducted an observational study analyzing the correlation between IgG N-glycans and aging, and age-related disease, T2DM.
Methods
This was an observational study with a longitudinal design. A community-based cohort in Beijing Xicheng district was established in 2012, and 701 participants aged from 23 to 68 (244 males and 457 females) were recruited. Demographic information and 39 clinical traits (7 anthropometric, 10 biochemical and 22 hematological) were collected and blood was sampled for plasma IgG N-glycans analysis using Ultra performance liquid chromatography (UPLC). From the glycan testing platform, 22 IgG N-glycan chromatographic peaks were detected and then 22 basic glycan traits (GP1-2, GP4-19, GP21-24) were calculated from the relative contributions of individual peaks to the total IgG N-glyans. Based on the 22 basic glycan traits, 54 derived glycan traits (17 derived basic glycan traits, 14 neutral glycan traits, 23 derived neutral glycan traits) were obtained for describing glycosylation character in details.
In 2014, a sample of 516 participants (160 males and 356 females), aged from 26 to 66 were re-investigated in a follow-up survey and the same clinical traits were collected. All the statistical analyses were performed with SPSS 23.0 and R programming language software. All reported P values were 2-sided and PN-glycans and level of fasting plasma glucose (FPG). Canonical correlation analysis (CAA) was used to determine the integrate correlation between IgG N-glycans and clinical traits. The potential of IgG N-glycans to be a biomarker of T2DM was analysed by receiver-operating characteristic (ROC) curve and decision tree methods. The relationship between IgG N-glycan traits and age was assessed by all-subsets regression, binominal regression and permutation test.
Results
1. Screening IgG N-Glycans for type 2 diabetes
A sample of 701 participants from the baseline survey was categorised into three groups according to their FPG levels: an NGR (normal glucose regulation) group (FPGN-glycans among the three groups of participants, the low level of galactosylation was found in people with high levels of FPG. The results of Spearman’s rank correlation analyses showed a decreased level of galactosylation with increasing of age. In addition, CCA indicated that IgG N-glycan traits highly correlated with the clinical traits such as age, waist circumference, waist-hip ratio, FPG, triglyceride, creatinine and urea (canonical correlations coefficient=0.662, P
A sample of 516 participants in the 2nd follow-up study was categorised into two groups according to the changes of their FPG (ΔFPG): A ΔFPG>0 group and a ΔFPG≤0 group. After comparison and correlation analysis of IgG N-glycans between the two groups, the low level of fucosylation was found in males with increased level of FPG (P
2. Association between IgG N-glycans and chronological and biological ages
As the correlation between IgG N-glycans and FPG altered with age, and age accounted for the largest canonical loading among risk factors of T2DM, we investigated the patterns of changes in IgG glycan profiles associated with age by analyzing IgG glycosylation in 701 community-based Han Chinese. Nineteen IgG N-glycan traits, including glycan peaks (GP) GP1, 2, 4-15, 17-19, 21, 23, change considerably with age and specific combinations of these glycan features, named “glycan age”, can explain 23.3% to 45.4% of the variance in chronological age in this population. In addition, the clinical traits (FPG and aspartate aminotransferase) which associated with biological age were strongly correlated with the “glycan age”.
Conclusions
1. IgG N-glycans showed significant variability in a Chinese Han population and significantly correlated with the level and the changes of FPG and other clinical traits associated with T2DM. The decision tree analysis could be appropriate in building a diagnostic model based on the IgG N-Glycan profiles to distinguish people with different FPG levels. Therefore, IgG N-glycosylation could be a potential early diagnostic biomarker of T2DM.
2. The study showed that most of IgG glycans were significantly related with chronological age in Han Chinese population and we investigated the extensive patterns of changes in IgG N-glycans with chronological age. The age predictive models consisted of 12 glycan structures and explained 23.2% to 45.4% of variance in chronological age. The remaining variance in these glycans was associated with the clinical traits related to biological age. Therefore, IgG glycosylation seems to correlate with both chronological and biological ages and thus contributes to the aging process. Models that can indicate biological age are of significant interest for prevention, diagnosis, and monitoring of aging and age-related diseases.
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Rudd, Pauline Mary. "The structure and function of glycoforms." Thesis, Open University, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.295604.
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Carpenet, Guéry Hélène. "Radiomarquage au 99mTc des IgA et IgG : optimisation du marquage, étude in vitro, biodistribution chez l'animal sain et sur modèle tumoral." Thesis, Limoges, 2015. http://www.theses.fr/2015LIMO0074/document.
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Depuis leur découverte en 1975 par Köhler et Milstein, le monde des Ac monoclonaux a beaucoup évolué. Ils occupent actuellement une place prépondérante dans la prise en charge de nombreux cancers. De nos jours, les Ac monoclonaux, ayant une AMM ou en essai clinique, sont tous de classe IgG voire IgG1. Cette classe d’Ac a cependant montré des limites à son utilisation, et l’étude d’autres isotypes d’Ac, comme les IgA, pourrait être intéressante. Les IgA, isotype d’Ac particulier en raison notamment de leur hétérogénéité dans les formes moléculaires, demeurent peu étudiées à l’instar des IgG. Dans ce travail, nous proposons un radiomarquage des IgA monomériques, polymériques et sécrétoires, avec le 99mTc par une méthode indirecte impliquant le 2-iminothiolane et le cœur tricarbonyl. Par le biais de ce radiomarquage, la biodistribution des IgA monomériques et polymériques après administration i.v. a été évaluée chez l’animal sain et chez l’animal porteur de tumeur à localisation muqueuse. Ces études nous ont permis d’entrevoir le potentiel diagnostique des IgA, mais aussi leur intérêt en thérapie ciblée de tumeurs à localisation muqueuse. D’autre part, grâce à leur résistance enzymatique et au phénomène de retranscytose, une nouvelle voie d’administration des Ac monoclonaux pourrait être développée. Dans cette optique, des IgA sécrétoires ont été administrées par voie orale lors d’études préliminaires de biodistributionSince their discovery in 1975, by Köhler and Milstein, monoclonal antibodies (mAbs) world has significantly evolved and they currently hold a prominent place in cancers care. Today, the mAbs, having a marketing authorization or in clinical trial, are all IgG class (IgG1). However, this Ab class showed limitations on its use, and the study of other isotypes, such as IgA, could be interesting. Unlike IgG, IgA, original isotype particularly because of their heterogeneity in molecular forms, remains understudied. In this work, we propose a radiolabeling of monomeric, polymeric and secretory IgA with 99mTc by an indirect method, involving 2-iminothiolane and tricarbonyl core. Biodistribution of radiolabeled monomeric and polymeric IgA was evaluated, after intravenous administration, in healthy animals and in mucosal tumor-bearing animals. These studies have allowed us to glimpse the IgA diagnostic potential, but also their interest in targeted therapy of tumors with mucosal localization. Moreover, thanks to their enzymatic strength and retranscytosis, a new administration route of mAbs could be developed. In this context, secretory IgA were administered orally in preliminary biodistribution studies
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Gildner, Theresa. "Life History Tradeoffs Between Testosterone and Immune Function Among Shuar Forager-Horticulturalists of Amazonian Ecuador." Thesis, University of Oregon, 2018. http://hdl.handle.net/1794/23822.
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The sex hormone testosterone supports male reproduction. However, testosterone is hypothesized to suppress immune activity, resulting in a tradeoff between energetic investment in reproductive effort and immune function. The Immunocompetence Handicap Hypothesis (ICHH) therefore argues that testosterone-linked masculine traits honestly signal health status to prospective mates, as only uninfected males should be able to maintain high testosterone levels. Still, this proposed tradeoff remains poorly tested among human men, especially among natural fertility populations experiencing high infectious disease burdens. This dissertation therefore tested the ICHH among indigenous Shuar men of Amazonian Ecuador. Specifically, this project examined testosterone variation patterns and assessed how male investment in reproductive effort is associated with reproductive success and immune function.
The first study tested testosterone level variation among Shuar men in relation to body composition, age, and style of life factors. This study demonstrated that age and BMI interactions shape testosterone levels in complex ways, such that the relationship between body composition and testosterone profile varies throughout the life course. The second study investigated whether individual reproductive success was significantly influenced by masculine trait development and parasite load. These results failed to support the hypotheses that masculine traits increased reproductive success or honestly signaled lack of parasitic disease. Instead, a significant positive association was observed between a composite score of masculine traits and Ascaris lumbricoides infection load; suggesting that male investment in reproductive effort may increase parasitic infection risk.
The final study assessed whether testosterone levels were negatively associated with four measures of immune function (parasite load, C-Reactive Protein [CRP], Immunoglobulin-G [IgG], and Immunoglobulin-E [IgE]). Testosterone levels were inversely associated with CRP levels and a positive relationship between testosterone levels and Trichuris trichiura infection load was documented, suggesting increased investment in reproductive effort may suppress some aspects of immune function and increase parasite burden. Overall, these studies fail to support the ICHH, but do indicate a context-dependent tradeoff between energetic investment in male reproductive effort and some aspects of immune function; thereby demonstrating complex interactions between physical characteristics, physiological processes, and immune activity in human men.
This dissertation includes unpublished, co-authored material.
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Beard, Lorraine Joyce. "IgG subclass concentrations in children in health and disease /." Title page, contents and summary only, 1990. http://web4.library.adelaide.edu.au/theses/09MD/09mdb368.pdf.
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