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Academic literature on the topic 'Immune serums Reactivity'

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Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "Immune serums Reactivity"

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Kaspruk, Lyudmila. "Organization of infection control in the Orenburg region in 1941– 1945: article dedicated to the 75th anniversary of the Great Victory." Spravočnik vrača obŝej praktiki (Journal of Family Medicine), no. 3 (March 1, 2020): 53–60. http://dx.doi.org/10.33920/med-10-2003-06.

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Risk of the spread of acute contagious infections at the front has sharply increased with the beginning of the Great Patriotic War of 1941–1945, as a result of which the problem of ensuring sanitary and epidemic wellbeing, including the home front, has been put forward. The issue of preventing the spread of infectious diseases was developed in the war period on the home front, by a group of scientists from the city of Chkalov as well. An important role was given to solving the problem of production of bactericides, improving the production of vaccines and serums, research to increase their immune properties, reduce reactivity, etc. Three institutes of epidemiology and microbiology functioned in the city of Chkalov (now Orenburg) in wartime: Smolensky, Chkalovsky and Ukrainian institute named after I. I. Mechnikov.
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Kysera, Ya V., Yu G. Storchak, and B. V. Gutyj. "Розробка імунопрофілактичного протипневмококового засобу та визначення його імуногенних властивостей на білих мишах." Ukrainian Journal of Ecology 8, no. 1 (February 13, 2018): 307–16. http://dx.doi.org/10.15421/2018_216.

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<p>In today's veterinary science and practice, the issue of creation and introduction into the production of diagnostic and preventive immunobiological remedies remains relevant. The prevention of factor bacterial diseases is one of the main tasks of veterinary medicine. Taking into account that sufficient immunobiological reactivity and resistance of an animal`s organism is provided by the optimal composition of vitamins, macro- and microelements, and insufficient vitamin, mineral, including micromineral nutrition causes in them a violation of the function of the immune system, reduces the resistance of the organism to infections, increases the incidence, emerged the issue of developing a new preventive and immunostimulant. It was established the specific composition of streptococci. Complex researches were carried out, which included the isolation of the most virulent pneumococci from the experimental material, taken in the animal farms of Lviv region in order to develop a preventive remedy "Pneumo-Pro" against pneumococcal infection. In 29% of cases there was S. pneumoniae, 21% – S. pyogenes, 17% – S. bovis, 12% – S. agalactiae and 21% – other streptococci streptococci streptococci strains that did not respond to streptococcal serums and were not identified as S. pneumoniae. Investigation of the sensitivity of pneumococci to antibiotics has shown that isolates have been sensitive to enrofloxacin, ofloxacin. Resistance to vancomycin, cefalexin, streptomycin, amoxicillin, ampicillin, cefazolin, lincomycin and erythromycin has been established. A non-harmful and immunogenic prophylactic remedy against pathogens of pneumococcal infection was tested with laboratory animals. During the development of a prophylactic remedy, the determination of its harmlessness was investigated immunogenic properties with different ratios of components of the preventive agent "Pneumo-Pro". White mice were used in the studies. The created preventive remedy "Pneumo-Pro" in its composition contains two components: Streptococcus pneumoniae and alcoholic extract of propolis, which possesses anti-inflammatory and immunomodulatory properties. Experimental studies have shown that propolis, due to its anti-inflammatory and antioxidant properties, prevents of pneumonia. Clinical observations showed the harmlessness of the experimental samples of the remedy: no swelling and abscesses at the injection site, no increase in local temperature and body temperature, a deterioration in the general condition of laboratory animals. The expediency of the use of immunoprophylaxis, made from local isolates of an infectious agent in order to increase the resistance of animals.</p>
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Gray, Julian C., Patrick H. Corran, Elena Mangia, Michael W. Gaunt, Qiuxiang Li, Kevin KA Tetteh, Spencer D. Polley, et al. "Profiling the Antibody Immune Response against Blood Stage Malaria Vaccine Candidates." Clinical Chemistry 53, no. 7 (July 1, 2007): 1244–53. http://dx.doi.org/10.1373/clinchem.2006.081695.

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Abstract Background: The complexity and diversity of the antibody immune response to the antigen repertoire of a pathogen has long been appreciated. Although it has been recognized that the detection of antibodies against multiple antigens dramatically improves the clinical sensitivity and specificity of diagnostic assays, the prognostic value of serum reactivity profiles against multiple microbial antigens in protection has not been investigated. Methods: Using malaria as a model we investigated whether antigen reactivity profiles in serum of children with different levels of clinical immunity to Plasmodium falciparum malaria correlated with protection. We developed a microarray immunoassay of 18 recombinant antigens derived from 4 leading blood-stage vaccine candidates for P. falciparum [merozoite surface protein 1 (MSP1), MSP2, MSP3, and apical membrane antigen (AMA)-1]. Associations between observed reactivity profiles and clinical status were sought using k-means clustering and phylogenetic networks. Results: The antibody immune response was unexpectedly complex, with different combinations of antigens recognized in different children. Serum reactivity to individual antigens did not correlate with immune status. By contrast, combined recognition of AMA-1 and allelic variants of MSP2 was significantly associated with protection against clinical malaria. This finding was confirmed independently by k-means clustering and phylogenetic networking. Conclusions: The analysis of reactivity profiles provides a wealth of novel information about the immune response against microbial organisms that would pass unnoticed in analysis of reactivity to antigens individually. Extension of this approach to a large fraction of the proteome may expedite the identification of correlates of protection and vaccine development against microbial diseases.
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Isla Larrain, Marina T., Andrea G. Colussi, Sandra O. Demichelis, Alberto Barbera, Aldo Cretón, Amada Segal-Eiras, and María V. Croce. "Humoral Immune Response against Tumoral Mucin 1 (MUC1) in Breast Cancer Patients." International Journal of Biological Markers 28, no. 3 (July 2013): 318–25. http://dx.doi.org/10.5301/jbm.5000036.

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The aim of this study was to elucidate whether the IgG humoral immune response to breast cancer cells is directed to the aberrant mucin-1 (MUC1) associated to this type of cancer. To this aim, an adaptation of immunohistochemistry (IHC) was performed on samples of 45 breast cancer tissues, 12 benign disease tissues, and 31 normal tissues, incubated with matched serum samples from the same patients. Each serum sample was also incubated, with a modified immunocytochemistry (ICC), with MCF7 cells. In both techniques, serum was employed instead of the primary antibody. In the case of IHC, the reactivity with sera diminished when added after previous incubation of the tumor/tissue with an anti-MUC1 mAb; the reduction in reactivity was: from 93% to 44% in breast cancer tissues, and from 100% to 67% in benign disease tissues. The reactivity of normal samples (36%) remained unchanged. In the case of ICC, the reactivity with sera decreased after incubation with anti-MUC1 mAb from 71% to 16% in breast cancer tissues, from 83% to 0% in benign disease tissues, and from 52% to 10% in normal serum samples. These results were confirmed employing siRNA MUC1 transient gene knockdown. By Western blot analysis – after immunoprecipitation (IP) of the circulating MUC1– and ELISA, the TF antigen was detected in circulating MUC1 in all breast cancer and benign samples while Tn was detected in 38% of the samples. The existence of IgG autoantibodies against aberrantly glycosylated MUC1 may have a protective role and may contribute to a better prognosis in some patients. Enhancement of this natural immune response may constitute an alternative therapeutic strategy.
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Schlame, Michael, Ivan Haller, Lisa Sammaritano, and Thomas Blanck. "Effect of Cardiolipin Oxidation on Solid-Phase Immunoassay for Antiphospholipid Antibodies." Thrombosis and Haemostasis 86, no. 12 (2001): 1475–82. http://dx.doi.org/10.1055/s-0037-1616751.

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SummaryDiagnostic assays for antiphospholipid antibodies are routinely performed on microtitre plates coated with cardiolipin. Here we show that contact between cardiolipin and NUNC-Immuno® plates leads to extensive oxidation, generating a series of peroxy-cardiolipins which were identified by electrospray ionization mass spectrometry. To investigate the impact of oxidation on the antibody assay, cardiolipin was resolved into 12 molecular species, including oxidized species and non-oxidized species with different degrees of unsaturation. All 12 species reacted under anaerobic conditions with serum from patients with primary antiphospholipid syndrome. Immune reactivity was similar for tetralinoleoyl-cardiolipin, trilinoleoyl-oleoyl-cardiolipin, and peroxycardiolipins, but somewhat lower for tristearoyl-oleoyl-cardiolipin. Oxidative treatment of cardiolipin with air, cytochrome c, or Cu2+/tert-butylhydroperoxide, either before or during the assay, did not enhance immune reactivity. Similar results were obtained with a monoclonal IgM from lupus-prone mice, that binds cardiolipin in the absence of protein cofactors. We conclude that the solid-phase assay for antiphospholipid antibodies can be supported by various oxidized and nonoxididized molecular species of cardiolipin.
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Maria, Vasco A. J., and Rui M. M. Victorino. "Hypersensitivity Immune Reaction as a Mechanism for Dilevalol-Associated Hepatitis." Annals of Pharmacotherapy 26, no. 7-8 (July 1992): 924–26. http://dx.doi.org/10.1177/106002809202600713.

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OBJECTIVE: To assess lymphocyte reactivity to dilevalol and to serum containing putative ex vivo dilevalol antigens or metabolites in a case of dilevalol-induced liver injury. PATIENT: A 58-year-old woman with a clinical diagnosis of dilevalol-induced liver injury. METHODS: Peripheral blood mononuclear cells collected from the patient were cultured in the presence of a solution of dilevalol and also with sera collected from a volunteer before and after dilevalol intake. A similar protocol was performed with lymphocytes from a healthy subject. RESULTS: No lymphocyte proliferation was observed either in the patient or in the healthy volunteer in the presence of dilevalol solutions. A significant proliferative response to serum collected after dilevalol intake was observed in the case of the patient compared with the proliferative response to the serum collected before the drug intake. No reactivity was found when lymphocytes from the healthy subject were tested under similar conditions. CONCLUSIONS: The methodology used allowed the detection of lymphocyte sensitization to sera containing ex vivo-prepared dilevalol antigens, suggesting the involvement of an immunologic mechanism in dilevalol-induced liver injury.
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Wijekoon, Suranji, Niranjala De Silva, Nayana Wijayawardhane, Madura Munasinghe, and Jayanthe Rajapakse. "Characterization of Antigenic Property of Adult Spirocerca lupi Collected from Esophageal Nodules in Dogs." INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY 17, no. 01 (January 25, 2021): 31–34. http://dx.doi.org/10.21887/ijvsbt.17.1.8.

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Spirocerca lupi, the esophageal nematode of dog , causes a potentially fatal disease in domestic dogs. Immunological techniques can identify the parasite proteins which elicit an immune response. However, the antigenic properties of S. lupi adult worms have not been fully understood. The immuno-reactivity of the naturally infected dog sera with the S. lupi somatic antigens showed 7 prominent immunoreactive bands of distinct sizes at 199, 148, 100, 87, 49, 16, and 12 kDa, whereas 8 prominent bands at 230, 200, 100, 60, 49, 29, 16, and 12 kDa against hyper immune serum raised in the rabbits. Common antigen bands for both types of serum were observed at 100, 49 , 16 and 12 kDa. Intriguingly, the current study was able to indicate the detailed antigenic profile with specific molecular moieties which might be a manifesto for future investigation. Identified specific antigens merit further analysis as potential tools for the evaluation of treatment success and prognosis of spirocercosis, and development of a sensitive and specific diagnostic test for early detection of S. lupi in infected dogs.
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Fitzgerald, Thomas. "Immobilization and neutralization of Treponema pallidum attached to cultured mammalian cells." Canadian Journal of Microbiology 31, no. 12 (December 1, 1985): 1152–56. http://dx.doi.org/10.1139/m85-217.

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The in vitro effects of antibodies, complement, and (or) macrophages on Treponema pallidum have been previously characterized using relatively simple systems of organisms incubated with the immune components. In vivo, the more complex environment may alter immune reactivity. Experiments were performed to determine whether immobilizing and neutralizing antibodies retained their effectiveness in a more complex environment involving cultured mammalian cells. Two different protocols were used. In protocol A treponemes and normal or immune serum were mixed and added immediately to the cultured cells. In protocol B treponemes were preincubated for 18 h with cultured cells to maximize treponemal attachment; then normal or immune serum was added. With both protocols, attachment of organisms resulted in less effecient immobilization and neutralization. In further experiments, cultured cells were disrupted with Triton X, leaving cytoskeletal remnants on the vessel surface. Identical immobilization and neutralization experiments were performed in the presence of these remnants. In contrast to the findings with viable cultured cells, treponemal attachment to these nonviable remnants did not effect either antibody reaction. Attached organisms were immobilized or neutralized just as efficiently as unattached organisms. Results are discussed in terms of the altered immune reactivity in more complex in vitro environments.
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Hartley, Carol A., Nino Ficorilli, Kemperly Dynon, Heidi E. Drummer, Jin-an Huang, and Michael J. Studdert. "Equine rhinitis A virus: structural proteins and immune response." Journal of General Virology 82, no. 7 (July 1, 2001): 1725–28. http://dx.doi.org/10.1099/0022-1317-82-7-1725.

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Equine rhinitis A virus (ERAV) is a picornavirus that has been reclassified as a member of the Aphthovirus genus because of its resemblance to foot-and-mouth disease virus at the level of nucleotide sequence and overall genomic structure. The N-terminal amino acid sequence of three of the four capsid proteins of ERAV was determined and showed that the proteolytic cleavage sites within the precursor P1 polypeptide occur exactly as those predicted for an aphthovirus-like 3C protease, which generates the capsid proteins VP1 and VP3. However, the autocatalytic cleavage site between VP4 and VP2, which is independent of 3C protease cleavage, was different from that predicted previously. ERAV.393/76 antisera from horses and rabbits showed different reactivity to the viral structural proteins in both serum neutralization assays and Western blots. High neutralizing antibody titres appeared to correlate with strong reactivity to VP1 in Western blots.
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Zuin, Jessica, Gianluca Veggiani, Paolo Pengo, Andrea Gallotta, Alessandra Biasiolo, Natascia Tono, Angelo Gatta, et al. "Evaluation of the Analytical Specificity of SCCA-IGM Assay for Monitoring Patients with Cirrhosis." International Journal of Biological Markers 24, no. 3 (July 2009): 209. http://dx.doi.org/10.1177/172460080902400335.

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Background and aim An increasing number of clinical data suggests the importance of the assessment of serum levels of the squamous cell carcinoma antigen (SCCA)-lgM immune complex for the diagnosis of hepatocellular carcinoma (HCC). In addition, monitoring of SCCA-IgM immune complexes has been described as a useful prognostic approach in patients with cirrhosis since the progressive increase of SCCA-IgM over time is associated with a higher risk of HCC development. Because other assays in patients with cirrhosis have been affected by interfering IgMs, the aim of the present study was to assess the specificity of SCCA-IgM reactivity in cirrhotic patients by evaluating SCCA-IgM detection dependence on different capturing phases and by measuring the recovery of SCCA-IgM reactivity after serum fractionation. Patients and methods Serum samples from 82 patients with cirrhosis (M/F ratio 3/1; mean age ± SD: 56 ± 9 years) were collected at the Liver Unit of the Department of Clinical and Experimental Medicine, University of Padua, according to the approved institutional procedures. Serum levels of SCCA-IgM were measured using two different ELISA tests: the reference assay based on a rabbit oligoclonal anti-human SCCA antibody (Hepa IC, Xeptagen, Italy) and a dedicated ELISA with a mouse monoclonal anti-SCCA as capture antibody. Results Serum levels of SCCA-IgM measured with the reference assay (median value 87 AU/mL) were higher than levels measured with the mouse monoclonal test (median value 78 AU/mL), but the differences were not statistically significant (Mann-Whitney U test, p>0.05). When SCCA-IgM levels measured with both tests were compared, a linear correlation was found (r = 0.77, p < 0.001). An in silico analysis using available structural data of SCCA showed the presence of up to three putative antigenic sites localized on the SCCA surface, thus providing evidence that the test with the mouse monoclonal anti-SCCA antibody could underestimate the total circulating immune complexes due to the steric hindrance of the enormous mass of IgM (900 kDa) that could mask on the SCCA (45 kDa) surface the binding site recognized by the monoclonal antibody. To show that the SCCA-IgM assay was not affected by interfering IgMs, we measured the recovery of SCCA-IgM reactivity after serum fractionation of ten of the most reactive samples in both assays. Total recovery of SCCA-IgM reactivity was obtained with both assays in the fractions corresponding to components with higher molecular weight than IgM and SCCA (>2000 kDa). Conclusions The results of this study indicate that the reactivity measured in cirrhotic patients is related only to SCCA-IgM immune complexes and is not affected by other serum components, supporting the analytical specificity of the SCCA-IgM assay and validating the importance of SCCA-IgM as a risk biomarker for HCC development.
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Book chapters on the topic "Immune serums Reactivity"

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"Natural Killer Cytotoxicity In a prospective study of colorectal cancer patients, the number of natural killer cells increased significantly in both transfused and untransfused patients who had potentially curative surgery (8). Natural killer cytotoxicity declined significantly in untransfused patients while increasing slightly in the transfused. Three months following surgery no differences in peripheral cell numbers or T cell subsets between the transfused and untransfused patients were noted. Removing leukocytes from the blood to be transfused abrogates the changes in natural killer cytotoxicity (9) These studies conflict with the findings of a prospective study of colorectal cancer (9). Patients were randomized to receive whole blood or filtered whole blood, removing 99.98% of the leukocytes and platelets. Natural killer cytotoxicity declined significantly in patients receiving unfiltered whole blood and remained significantly depressed 30 days following surgery. Natural killer cytotoxicity in untransfused patients and in patients receiving filtered blood declined significantly with surgery but fully recovered by 30 days. Since declines in natural killer cytotoxicity can be prevented in cancer patients by simply filtering blood and since natural killer cytotoxicity is of proven prognostic significance, filtered blood may improve the outcome for patients with malignancies. Immune Function Following Transfusion of Dialysis Patients The effect of transfusion on dialysis patients is both immune enhancing and immune suppressing. Transfusion is followed by the appearance of antibodies to antigens present on the cells of the transfused blood and these antibodies are capable of killing lymphocytes having these antigens. Lymphocytotoxic antibodies are responsible for early graft failures and their appearance is called sensitization. Immune suppression accompanies sensitization. Suppressor lymphocytes begin to appear in the serum of transfusion recipients at the same time lymphocytotoxic antibodies are appearing and their appearance is probably also mediated by antibodies -antibodies which play a role in regulating immune function. Suppresser cells suppress lymphocyte responses to antigens on the cells of transfused blood and on the cells of the transplant. Lymphocyte suppression following blood transfusion may be permanent or transient, but in most recipients the degree of suppression is enhanced by additional blood transfusions and probably maintained by the presence of the allograft following transplant. In dialysis patients who receive blood transfusions lymphocyte responses to antigens, mitogens and homologous lymphocytes decline to 50% one week following a single unit of blood in comparison to lymphocyte reactivity measured immediately before transfusion (10). Lymphocyte reactivity declines progressively with additional units of blood given and returns to pretransfusions levels if blood is withheld for six weeks. Suppresser activity is enhanced following transfusion but not at one week when lymphocyte reactivity is at its lowest point, indicating that suppressive activity and lymphocyte inhibition are separate events (11). Finally, natural killer cytotoxicity is significantly reduced following transfusion of dialysis patients and remains low following transplant, although a correlation between natural killer cytotoxicity and graft survival has not been shown (12)." In Transfusion Immunology and Medicine, 294. CRC Press, 1995. http://dx.doi.org/10.1201/9781482273441-23.

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Conference papers on the topic "Immune serums Reactivity"

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Hamza, Shaimaa, Ekaterina Evgenevna Garanina, Ekaterina Vladimirovna Martynova, Maria Ivanovna Markelova, Jurij Nikolaevich Davidyuk, Venera Gusmanovna Shakirova, and Svetlana Francevna Khaiboullina. "Antibody Immune Responses to SARS-CoV-2 Peptides in COVID-19 Convalescent Patients." In All-Russian scientific conference with International Participation. Publishing house Sreda, 2022. http://dx.doi.org/10.31483/r-102484.

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Identifying immunogenic targets of SARS-CoV-2 is essential to develop novel treatments. The authors of the article studied humoral immune responses to spike (S) and nucleocapsid (N) SARS-CoV-2 proteins in serum from convalescent COVID-19 patients from Tatarstan, Russia. Multiple SARS-CoV-2 peptides were identified as reacting with convalescent COVID-19 serum. In addition, age and gender associated differences in the reactivity to S and N protein peptides were identified. Changing pattern of immunogenic peptide reactivity in COVID-19 serum based on age and gender was demonstrated. These data highlight how humoral immune responses to some of these peptides could contribute to SARS-CoV-2 pathogenesis.
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Beardsley, D. S. "IMMUNE THROMBOCYTOPENIA (ITP) : PLATELET TARGET ANTIGENS OF THE ANTIBODIES IN DIFFERENT CLINICAL SETTINGS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644757.

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Autoantibodies against platelet antigens are important in the pathogenesis of ITP. We have studied the antigenic targets of these autoantibodies by immunoblotting using electrophoretically separated proteins from normal, Glanzmann's Thrombasthenic, and Bernard-Soulier Syndrome platelets immobilized on nitrocellulose paper. Incubation of these proteins with ITP patient serum, immunoglobulin, or F(ab*>2, followed by labeled antiglobulin, allowed identification of the antigenic target glycoproteins (GPfs). Patients with ITP of several categories were studied: A) Chronic ITP (> 1 yr duration) including a subset of patients who hadmild thrombocytopenia but significant hemorrhagic manifestations. B) Acute ITP (<6 mo) following varicella infectionAcute onset ITP unresponsive to any of the usual therapeutic modalities. A majority of patients in group A (32/48) had antibodies directed against a protein with a MW of lOOkD. In some cases, the target antigen was localized to GPIIIa specifically by absence of reactivity with Type I Glanzmann's thrombasthenic platelets known to be totally deficient in GPIIb and Ilia. Two individuals in the subset with unexpectedly severe hemorrhagic symptoms also had anti-GPIIIa antibodies, but the antigenic target was different since only these two antibodies could be shown to interfere with binding of 125j_fibrinogen to normal plateletsIn contrast to the individuals with anti-GPIIIa antibodies, all group B patients (7/7) with acute post-varicella ITP had antibodies directed against an 85kD thrombin sensitive protein, and two of the three group C individuals studied showed antibodies directed against GPIb. These studies indicate that a number of different platelet antigens can be the targets of platelet autoantibodies in ITP. Results suggest that antibodies against a particular antigen may correlate with a similar clinical setting in different patients. Further work will determine whether antigen identification at the time of diagnosis can be used to predict the clinical course for an individual patient
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MacGregor, I. R., N. A. Booth, N. R. Hunter, and B. Bennet. "QUANTIFICATION OF ENDOTHELIAL PLASMINOGEN ACTIVATOR INHIBITOR (PAI-1) ANTIGEN BY AN ENZYME LINKED IMMUNOSORBENT ASSAY (ELISA)." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644450.

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An ELISA to measure PAI-1 antigen has been developed using PAI-1 purified from human endothelial cell conditioned medium and a monospecific antiserum raised against it in the rabbit. Test and standard samples diluted in assay buffer containing non-immune rabbit serum were incubated in microplate wells coated with anti-PAI-1 IgG. Then biotinylated anti-PAI-1 IgG was added to the wells followed by a streptavidin-biotinylated horseradish peroxidase complex. Tetramethylbenzidine was used as substrate and the optimised ELISA had a detection limit of 0.2 ng PAI-1 ml-1 sample, using purified PAI-1 of known concentration as a standard for the assay.PAI-1 antigen was readily detectable in human plasmas and higher concentrations were invariably detected in the corresponding whole blood sera.α2antiplasmin and antithrombin III which have been shown to have high degrees of sequence homology with PAI-J were undetectable in the ELISA at concentrations of 10 ug ml-1 . Sera from baboon and Rhesus and Cynomologus monkeys exhibited partial cross reactivity in the ELISA while no crossreactivities were observed with a wide range of non-primate sera that included dog, goat, cow, horse, pig and rat.A variety of human cell cultures were assayed for PAI-1 antigen. Endothelial cells from umbilical cord veins and arteries and from adult sapgenous veins secreted, typically,1-2 ug PAI-1 24-1 hr per 10 cells. This represented approximately 2-4% of the total secreted protein. Detectable but considerably lower levels of PAI-1 were secreted by keratinocytes and fibroblasts as well as a lung carcinoma cell line A549.No PAI-1 was detected in media conditioned by two melanoma cell lines, a breast carcinoma cell line MCF7 or a monocytic leukaemia JIII. The ELISA proved suitable for monitoring altered cellular PAI-1 metabolism, and in conjunction with functional assays, for investigating changes in PAI-1 specific activity.
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Krasnoshtanova, Alla, and Alesya Yudina. "PRODUCTION OF ANTIBODIES FROM POULTRY YOLK (IgY) AND INVESTIGATION OF THEIR IMMUNOCHEMICAL PROPERTIES." In GEOLINKS Conference Proceedings. Saima Consult Ltd, 2021. http://dx.doi.org/10.32008/geolinks2021/b1/v3/17.

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"A particularly important aspect of immunology is to develop non-invasive methods of obtaining antibodies which could be a great alternative to traditional ones that based on the harmful procedure of isolation of immunoglobulins from animal blood sera. That’s why the extraction of antibodies from poultry egg yolks (IgY) is the most promising. Due to the fact of variation of IgY structural features that determine the definite immunochemical properties, yolk antibodies in comparison with mammalian immunoglobulins (IgG) does not interact with rheumatoid factor (Rf), contribute to the activation of the complement system, bind to the Fc-receptor (FcR), and also has weak cross-reactivity, which confirms the possibility of their widespread use in medicine and food. Also the presence of phylogenetic distance between chickens and mammalians guarantees immune response against conservative mammalian protein molecules which is highly important for the creation of new generation test systems. The aim of this work is to develop a selective method of producing high-purity immunoglobulin Y preparations from the yolk of chicken eggs. There were adopted selective conditions of isolation of IgY under spontaneous thawing procedure at the room temperature of firstly frozen yolk solution in a sodium-phosphate buffer mixed with water (pH 5.0) in a ratio of 1:6, which leads to receiving a water-soluble fraction further precipitated with the sodium chloride at a concentration of 10% of the solution mass and subsequently concentrated using ultrafiltration with membrane UAM-10, that allows achieving the content of IgY not less than 95% per dry substance in immunoglobulin fraction. It is possible to produce a protein fraction with a protein content of at least 9 g/l. The purity of the immunoglobulin fraction was verified using polyacrylamide gel electrophoresis. The presence of a light chain in the IgY solution was proved to be a low-molecular compound using the method of gel-filtration-chromatography. The immunological activity of IgY was studied with respect to bovine serum albumin (BSA) as an antigen. The enzymatic resistance of IgY against proteolytic enzymes was tested in area of the gastrointestinal tract."
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