Relevant bibliographies by topics / Immortalized cell line

Academic literature on the topic 'Immortalized cell line'

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Published: 9 March 2023
Last updated: 10 March 2023

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Journal articles on the topic "Immortalized cell line"

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Nitta, K., and N. Horiba. "An immortalized rat mesangial cell line." In Vitro Cellular & Developmental Biology - Animal 33, no. 3 (March 1997): 156–57. http://dx.doi.org/10.1007/s11626-997-0134-y.

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Nagai, Atsushi, Seiji Mishima, Yuri Ishida, Hiroto Ishikura, Takayuki Harada, Shotai Kobayashi, and Seung U. Kim. "Immortalized human microglial cell line: Phenotypic expression." Journal of Neuroscience Research 81, no. 3 (August 1, 2005): 342–48. http://dx.doi.org/10.1002/jnr.20478.

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Nakamura, Yukio, Takashi Hiroyama, Kenichi Miharada, and Ryo Kurita. "Red blood cell production from immortalized progenitor cell line." International Journal of Hematology 93, no. 1 (December 25, 2010): 5–9. http://dx.doi.org/10.1007/s12185-010-0742-2.

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Velazquez, Jessica, Amel Sengal, Carl E. Allen, and Rikhia Chakraborty. "Creating and Testing a CD207+ Langerhans Cell Histiocytosis-like Cell Line." Blood 134, Supplement_1 (November 13, 2019): 5399. http://dx.doi.org/10.1182/blood-2019-130588.

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Langerhans cell histiocytosis (LCH) is an inflammatory myeloid neoplasia characterized by pathological CD207+ dendritic cells (DCs) with persistent mitogen-activated protein kinase (MAPK) activation. Investigations into LCH have historically been challenged by the small percentage of pathologic CD207+ DCs. No cell lines with morphology or function representing LCH lesion CD207+ DCs currently exist. We utilized four strategies to generate cell line(s) mimicking differentiated LCH pathogenic cells. First, CD207+ cells were isolated from the lesion of an LCH patient and cultured in a cytokine cocktail. The cells maintained CD1a and CD207 expression for two weeks, after which there were significant changes in cell morphology and progression to cell death. When a similar approach was implemented to isolate and culture skin CD207+ cells, the cells were viable for only three days, supporting the potential role for somatic activating MAPK mutations in LCH lesion DCs to prolong viability. CD207+ cells were then isolated from HLA-DR+ (lineage negative) cells from the lymphoid stroma of healthy tonsils (tCD207+). The tCD207+ cells were transduced with a lentivirus encoding human telomerase reverse transcriptase (hTERT). These cells, also lacking MAPK activating mutations, died two weeks post-transduction. A fourth approach has been more successful in which tCD207+ cells were cultured in a cytokine cocktail which provided MAPK pathway stimulation. The cells retained CD207 expression and survived in culture for over two weeks. The cells were then immortalized using a lentivirus encoding HOXA9. Immortalized cells maintained CD207 expression. Allele specific MAPK pathway mutations (BRAF and MAP2K1) are being generated by class II CRISPR/Cpf1 genome editing as Cpf1 has been shown to have robust activity to induce specific disruption of only mutant, but not wild-type, BRAF allele. The phenotypic and genomic characteristics of the immortalized cells expressing the different MAPK pathway mutations will be assessed by RHG-banding cytogenetic analysis, fluorescence in situ hybridization, gene expression analysis, and immuno‐cytochemistry and results will be compared to cells isolated from LCH lesions to confirm whether the established cell line may be a viable in vitro mimic of the LCH lesion DC. Disclosures No relevant conflicts of interest to declare.
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Steele, Stacy L., Yongren Wu, Robert J. Kolb, Monika Gooz, Courtney J. Haycraft, Kent T. Keyser, Lisa Guay-Woodford, Hai Yao, and P. Darwin Bell. "Telomerase immortalization of principal cells from mouse collecting duct." American Journal of Physiology-Renal Physiology 299, no. 6 (December 2010): F1507—F1514. http://dx.doi.org/10.1152/ajprenal.00183.2010.

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Recently, the use of overexpression of telomerase reverse transcriptase (TERT) has led to the generation of immortalized human cell lines. However, this cell immortalization approach has not been reported in well-differentiated mouse cells, such as renal epithelial cells. We sought to establish and then characterize a mouse collecting duct cell line, using ectopic expression of mTERT. Isolated primary cortical collecting duct (CCD) cell lines were transduced with mouse (m)TERT, using a lentiviral vector. mTERT-negative cells did not survive blasticidin selection, whereas mTERT-immortalized cells proliferated in selection media for over 40 subpassages. mTERT messenger RNA and telomerase activity was elevated in these cells, compared with an SV40-immortalized cell line. Flow cytometry with Dolichos biflorus agglutinin was used to select the CCD principal cells, and we designated this cell line mTERT-CCD. Cells were well differentiated and exhibited morphological characteristics typically found in renal epithelial cells, such as tight junction formation, microvilli, and primary cilia. Further characterization using standard immunofluorescence revealed abundant expression of aquaporin-2 and the vasopressin type 2 receptor. mTERT-CCD cells exhibited cAMP-stimulated/benzamil-inhibited whole cell currents. Whole cell patch-clamp currents were also enhanced after a 6-day treatment with aldosterone. In conclusion, we have successfully used mTERT to immortalize mouse collecting duct cells that retain the basic in vivo phenotypic characteristics of collecting duct cells. This technique should be valuable in generating cell lines from genetically engineered mouse models.
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Tan, Jia-Jie, Lu Wang, Ting-Ting Mo, Yuan-Feng Dai, Juan Lu, Xiong Liu, Huai-Hong Chen, Wen-Dong Tian, and Xiang-Ping Li. "Establishment of Immortalized Laryngeal Epithelial Cells Transfected with Bmi1." Cell Transplantation 29 (January 1, 2020): 096368972090819. http://dx.doi.org/10.1177/0963689720908198.

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Primary laryngeal epithelial cells are essential to exploring the mechanisms of laryngeal and voice disorders; however, they are difficult to study and apply because of their limited life span. The purpose of this study was to develop a stable and reliable in vitro model for the comprehensive study of the pathogenesis of laryngeal and voice diseases. The pLVTHM-Bmi1 plasmid was constructed and used to immortalize primary laryngeal epithelial cells by lentiviral infection. The expressions of Bmi1, human telomerase reverse transcriptase (hTERT), p53, and pRB pathway proteins were detected by western blotting. Functional characteristics of the immortalized cell lines were verified by cell senescence β-galactosidase staining, 5-ethynyl-2′-deoxyuridine cell proliferation test, and flow cytometry. We successfully introduced Bmi into human subglottic (hSG) cells and human ventricle (hV) cells. Both the human immortalized subglottic Bmi1 (hSG-Bmi1) cell line and the human immortalized ventricle Bmi1 (hV-Bmi1) cell line maintained normal epithelial morphology and divided successfully after more than 20 culture passages. As Bmi1 was overexpressed in these cells, the expression of human telomerase reverse transcriptase (hTERT) and phosphorylated Rb increased while p16 and p21 decreased. Following Bmi1-mediated immortalization, cell senescence decreased significantly, and cell proliferation was accelerated. Tumor formation was not observed for hSG, hV, or hSG-Bmi1, and hV-Bmi1 cells in nude mice. hSG-Bmi1 cells dominated by stratified squamous epithelium and hV-Bmi1 cells dominated by columnar cells were established. The new cell lines lay a foundation for the study of the pathogenic mechanisms of laryngeal and voice diseases.
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Winn, Shelley R., Gannon Randolph, Hasan Uludag, Shou C. Wong, Gregory A. Hair, and Jeffrey O. Hollinger. "Establishing an Immortalized Human Osteoprecursor Cell Line: OPC1." Journal of Bone and Mineral Research 14, no. 10 (October 1, 1999): 1721–33. http://dx.doi.org/10.1359/jbmr.1999.14.10.1721.

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Zhang, H., Y. Zhao, Y. Li, and F. Tian. "Establishment of immortalized epithelial cell line of endometrium." Fertility and Sterility 77 (February 2002): S32. http://dx.doi.org/10.1016/s0015-0282(01)03112-0.

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Levashova, Zoia B., Sergei Y. Plisov, and Alan O. Perantoni. "Conditionally immortalized cell line of inducible metanephric mesenchyme." Kidney International 63, no. 6 (June 2003): 2075–87. http://dx.doi.org/10.1046/j.1523-1755.2003.00010.x.

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Newman, S. L., A. A. Weikle, T. J. Neuberger, and J. W. Bigbee. "Myelinogenic potential of an immortalized oligodendrocyte cell line." Journal of Neuroscience Research 40, no. 5 (April 1, 1995): 680–93. http://dx.doi.org/10.1002/jnr.490400514.

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Dissertations / Theses on the topic "Immortalized cell line"

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Valtink, Monika, Rita Gruschwitz, Richard H. W. Funk, and Katrin Engelmann. "Two Clonal Cell Lines of Immortalized Human Corneal Endothelial Cells Show either Differentiated or Precursor Cell Characteristics." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-136199.

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Access to primary human corneal endothelial cells (HCEC) is limited and donor-derived differences between cultures exacerbate the issue of data reproducibility, whereas cell lines can provide sufficient numbers of homogenous cells for multiple experiments. An immortalized HCEC population was adapted to serum-free culture medium and repeated cloning was performed. Clonally grown cells were propagated under serum-free conditions and growth curves were recorded. Cells were characterized immunocytochemically for junctional proteins, collagens, Na,K-ATPase and HCEC-specific 9.3.E-antigen. Ultrastructure was monitored by scanning and transmission electron microscopy. Two clonal cell lines, HCEC-B4G12 and HCEC-H9C1, could be isolated and expanded, which differed morphologically: B4G12 cells were polygonal, strongly adherent and formed a strict monolayer, H9C1 cells were less adherent and formed floating spheres. The generation time of B4G12 cells was 62.26 ± 14.5 h and that of H9C1 cells 44.05 ± 5.05 h. Scanning electron microscopy revealed that B4G12 cells had a smooth cell surface, while H9C1 cells had numerous thin filopodia. Both cell lines expressed ZO-1 and occludin adequately, and little but well detectable amounts of connexin-43. Expression of HCEC-specific 9.3.E-antigen was found commensurately in both cell lines, while expression of Na,K-ATPase α1 was higher in H9C1 cells than in B4G12 cells. B4G12 cells expressed collagen IV abundantly and almost no collagen III, while H9C1 cells expressed both collagens at reasonable amounts. It is concluded that the clonal cell line B4G12 represents an ideal model of differentiated HCEC, while H9C1 may reflect features of developing or transitional HCEC
Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich
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Valtink, Monika, Rita Gruschwitz, Richard H. W. Funk, and Katrin Engelmann. "Two Clonal Cell Lines of Immortalized Human Corneal Endothelial Cells Show either Differentiated or Precursor Cell Characteristics." Karger, 2008. https://tud.qucosa.de/id/qucosa%3A27701.

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Access to primary human corneal endothelial cells (HCEC) is limited and donor-derived differences between cultures exacerbate the issue of data reproducibility, whereas cell lines can provide sufficient numbers of homogenous cells for multiple experiments. An immortalized HCEC population was adapted to serum-free culture medium and repeated cloning was performed. Clonally grown cells were propagated under serum-free conditions and growth curves were recorded. Cells were characterized immunocytochemically for junctional proteins, collagens, Na,K-ATPase and HCEC-specific 9.3.E-antigen. Ultrastructure was monitored by scanning and transmission electron microscopy. Two clonal cell lines, HCEC-B4G12 and HCEC-H9C1, could be isolated and expanded, which differed morphologically: B4G12 cells were polygonal, strongly adherent and formed a strict monolayer, H9C1 cells were less adherent and formed floating spheres. The generation time of B4G12 cells was 62.26 ± 14.5 h and that of H9C1 cells 44.05 ± 5.05 h. Scanning electron microscopy revealed that B4G12 cells had a smooth cell surface, while H9C1 cells had numerous thin filopodia. Both cell lines expressed ZO-1 and occludin adequately, and little but well detectable amounts of connexin-43. Expression of HCEC-specific 9.3.E-antigen was found commensurately in both cell lines, while expression of Na,K-ATPase α1 was higher in H9C1 cells than in B4G12 cells. B4G12 cells expressed collagen IV abundantly and almost no collagen III, while H9C1 cells expressed both collagens at reasonable amounts. It is concluded that the clonal cell line B4G12 represents an ideal model of differentiated HCEC, while H9C1 may reflect features of developing or transitional HCEC.
Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
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Thieme, Sebastian, Alexander Holzbaur, Ralf Wiedemuth, Aline Binner, Katrin Navratiel, Konstantinos Anastassiadis, Sebastian Brenner, and Cornelia Richter. "The Dox-pDC - A murine conditionally immortalized plasmacytoid dendritic cell line with native immune profile." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2018. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-234947.

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Plasmacytoid dendritic cells (pDC) constitute a very rare blood cell population and play a significant role in immune response and immune-mediated disorders. Investigations on primary pDCs are hindered not only due to their rarity but also because they represent a heterogeneous cell population which is difficult to culture ex vivo. We generated a conditionally immortalized pDC line (Dox-pDC) from mice with Doxycycline-inducible SV40 Large T Antigen with a comparable immune profile to primary pDCs. The Dox-pDC secrete pro- and anti-inflammatory cytokines upon Toll-like receptor 9 stimulation and upregulate their MHCI, MHCII and costimulatory molecules. Further, the Dox-pDC activate and polarize naïve T cells in vivo and in vitro in response to the model antigen Ovalbumin. Due to their long-term culture stability and their robust proliferation Dox-pDC represent a reliable alternative to primary mouse pDC.
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Yin, Zhanhai. "Establishment of a clonal immortalized human mesenchymal stem cell line expressing hTERT using lentiviral gene transfer." Diss., lmu, 2012. http://nbn-resolving.de/urn:nbn:de:bvb:19-145290.

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HOSHINO, MUNEMITSU, MUTSUSHI MATSUYAMA, OSAMU TAGUCHI, MORIAKI KUSAKABE, WORAWIDH WAJJWALKU, JIN LU, TOYOHARU YOKOI, et al. "Establishment and Characterization of Immortalized Non-Transplantable Mouse Mammary Cell Lines Cloned from a MMTV-induced Tumor Cell Line Cultured for A Long Duration." Nagoya University School of Medicine, 1991. http://hdl.handle.net/2237/17515.

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Wang, Tianren. "The role of SHIP2 in suppressing inflammatory signaling induced by LPS in immortalized murine macrophage cell line." Thesis, University of British Columbia, 2016. http://hdl.handle.net/2429/56632.

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Inflammation is an important step in the body’s defense against pathogen infection. However, it must be tightly regulated and appropriately terminated to prevent pathological consequences. Interleukin-10 (IL10) is one of the body’s most important anti-inflammatory cytokine that can inhibit many molecular events necessary for promoting inflammation including production of pro-inflammatory cytokines such as Tumor Necrosis Factor α (TNFα). Our laboratory has recently shown that SH2-domain containing Inositol 5ʹ phosphatase (SHIP1) is involved in IL10 signaling in macrophages, and although the mechanism of how this occurs is not well studied, our laboratory have obtained data suggesting SHIP1 mediates IL10 signalling through its phosphatase activity or interaction with other signalling proteins. SHIP2 is the only other known homologue of SHIP1 with approximately 38% amino acid sequence identity, yet they possess several similar functions including mediating FcγIIB signaling and phagocytosis. Because of their similarities and SHIP1’s involvement in IL10 signaling, we sought to investigate whether SHIP2 is also involved in inhibiting inflammatory response in macrophage by knocking it out using CRISPR/Cas9-mediated genome editing. Overall, we were unable to determine whether SHIP2 plays a role in macrophage anti-inflammatory response due to the large variation in cell sensitivity to IL10 and we also observed that transduction of macrophages with CRISPR/Cas9 virus alters the cellular response to IL10 which confounded our investigation of SHIP2 function.
Medicine, Faculty of
Biochemistry and Molecular Biology, Department of
Graduate
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Rekus, Martin T. "Characterization of growth and differentiation of a spontaneously immortalized keratinocyte cell line (HaCaT) in a defined, serum free culture system." [S.l.] : [s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=963505262.

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Yin, Zhanhai [Verfasser], and Wolf [Akademischer Betreuer] Mutschler. "Establishment of a clonal immortalized human mesenchymal stem cell line expressing hTERT using lentiviral gene transfer : no / Zhanhai Yin. Betreuer: Wolf Mutschler." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2012. http://d-nb.info/1024658546/34.

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Otsuka, Toshiyuki. "Regulated expression of neurogenic basic helix-loop-helix transcription factors during differentiation of the immortalized neuronal progenitor cell line HC2S2 into neurons." Kyoto University, 1998. http://hdl.handle.net/2433/182245.

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CAMBIANICA, ILARIA NADIA. "In vitro blood brain barrier models as a screening tool for brain targeted nanobased drug delivery systems." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2012. http://hdl.handle.net/10281/39834.

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The blood brain barrier (BBB) is a selective biological barrier located at the brain capillaries, that protects the central nervous system (CNS) by monitoring exchanges between blood and brain. The BBB controls and regulates the composition of the CNS environment and it still constitutes the main obstacle for drug delivery to the brain (Weiss N. et al., 2009). The significant scientific and industrial interest in the physiology and pathology of the BBB led to the development of vast number of in vitro BBB models. Even though no “ideal” model exists yet, some of the currently available ones are very useful to investigate permeability, transport mechanisms and cellular and molecular events which occur at the BBB level. New strategies for brain targeted drug delivery exploit endogenously expressed transporters to elicit drug passage across the BBB. Among them, nanoparticles represent a promising tool, since they are biocompatible and biodegradable, and they can be functionalized to target the BBB (De Boer A.G. and Gaillard P.J., 2007) (Beija M et al., 2012; Caruthers S.D. et al., 2007; Moghimi S.M. et al., 2005). In this study we settled in vitro BBB models to identify, with high-throughput screening, the most promising nanoliposomes (NL) for combined BBB crossing and binding of amyloid peptides, for joint therapy and diagnosis of Alzheimer’s disease (AD). Firstly, we characterized two in vitro models of BBB, based on immortalized cell lines of human and rat origin, the hCMEC/D3 and RBE4 cells, respectively. We tested the transendothelial electrical resistance (TEER) and the endothelial permeability (PE) of small hydrophilic compounds: our results, in agreement with data reported in literature, lead us to conclude that these cellular models are suitable for their employment as high-throughput screening tools. Subsequently, we tested NL mono-functionalized with three different peptides, the apolipoproteinE derived peptide (the ApoE monomer (mApoE), amino acids 141-150), its tandem dimer (dApoE) (141-150)2, and the Human Immunodeficency Virus type 1 (HIV-1) transactivator of transcription (TAT) peptide. We evaluated their uptake and PE; we selected the TAT functionalization as the best performing concerning cellular uptake, and the mApoE functionalization when considering both the internalization and PE. Once assessed the dynamics of mono-functionalized NL interactions with endothelial cells, we investigated mApoE- and dApoE-NL loading a curcumin-derivative (Re F. et al., 2011) to bind Aβ. We clearly demonstrated that the mApoE-functionalization allows a better drug cellular internalization, whereas dApoE-NL enhances drug PE at the highest extent. We then considered mApoE- and dApoE-NL exposing the Aβ targeting ligands phosphatidic acid (PA) or cardiolipin (CL), demonstrating that PA-mApoE-NL showed the highest cellular uptake and PE. We also studied TAT-NL exposing curcumin derivative3 (Airoldi C. et al., 2011) for Aβ binding, clearly indicating that TAT functionalization increased cellular uptake and PE of curcumin derivative3-NL. We also studied intracellular fate of NL double functionalized, exposing Aβ targeting ligands, and no co-localization was detected with acidic cellular compartments, suggesting that NL may escape from lysosomal degradative pathway. Taken together, these results indicate that the formulations herein analyzed are suitable tools for brain targeted drug and contrast agent delivery. We suggest further development of mApoE and dApoE-NL entrapped with a drug payload for their employment as BBB endothelial cell or brain targeted drug delivery tools, respectively. We also selected PAmApoE-NL and curcumin derivative3-TAT-NL as promising tools for their employment in combination for AD therapy and diagnosis. Further studies, based also on in vivo experiments, are needed to evaluate NL suitability for clinical exploitation. Finally, we inquired the endocytic mechanisms that mediates the entry of NL in the endothelial cells of BBB. We employed RNA interference technique to down-regulate caveolin1 expression. Our preliminary data suggest that caveolin1 and the related caveolaemediated endocytosis pathway may account for 40% of mApoE-NL cellular uptake. Future directions regard the down-regulation of other proteins specifically involved in different endocytic mechanisms, i.e. clathrin-mediated and adsoprptive endocytosis, in order to assess which endocytic mechanisms may account for ApoE and TAT-NL internalization.
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Books on the topic "Immortalized cell line"

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Ian, Freshney R., and Freshney Mary G, eds. Culture of immortalized cells. New York: Wiley-Liss, 1996.

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Book chapters on the topic "Immortalized cell line"

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Xu, Wen’an, and Shuo Chen. "Establishment of an Immortalized Mouse Bmp2 Knockout Dental Papilla Mesenchymal Cell Line." In Methods in Molecular Biology, 13–19. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9012-2_2.

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Abbott, N. Joan, Pierre-Olivier Couraud, Françoise Roux, and David J. Begley. "Studies on an Immortalized Brain Endothelial Cell Line: Characterization, Permeability and Transport." In New Concepts of a Blood—Brain Barrier, 239–49. Boston, MA: Springer US, 1995. http://dx.doi.org/10.1007/978-1-4899-1054-7_24.

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Kibble, Emily A., Mitali Sarkar-Tyson, Geoffrey W. Coombs, and Charlene M. Kahler. "The Detroit 562 Pharyngeal Immortalized Cell Line Model for the Assessment of Infectivity of Pathogenic Neisseria sp." In Methods in Molecular Biology, 123–33. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9202-7_9.

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Hasegawa, Tomokazu, Naoyuki Chosa, Takeyoshi Asakawa, Yoshitaka Yoshimura, Akira Ishisaki, and Mitsuro Tanaka. "Establishment of Clonal Periodontal Ligament Cell Line Derived from Deciduous Tooth Immortalized by Human Telomerase Reverse Transcriptase (hTERT) Gene Transfer." In Interface Oral Health Science 2011, 114–16. Tokyo: Springer Japan, 2012. http://dx.doi.org/10.1007/978-4-431-54070-0_25.

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Walther, N., M. Jansen, S. Ergün, B. Kascheike, G. Tillmann, and R. Ivell. "Sertoli Cell-Specific Gene Expression in Conditionally Immortalized Cell Lines." In Advances in Experimental Medicine and Biology, 139–42. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4615-5913-9_22.

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MacDonald, C., F. Reid, K. Anderson, L. Yin, E. Hill, H. Kelly, and M. H. Grant. "Metabolism and Toxicological Studies in Immortalised Rat Hepatocyte Cell Lines." In Animal Cell Technology, 73–78. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-011-5404-8_12.

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Verbruggen, G., A. M. Malfait, K. F. Almgvist, E. M. Veys, S. Thenet, B. Benoit, S. Demignot, A. Tachet des Combes, and M. Adolphe. "Development of Immortalized Human Articular Cartilage Cell Lines." In Joint Destruction in Arthritis and Osteoarthritis, 267–72. Basel: Birkhäuser Basel, 1993. http://dx.doi.org/10.1007/978-3-0348-7442-7_33.

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Tai, Guanping, Peter Hohenstein, and Jamie A. Davies. "Making Immortalized Cell Lines from Embryonic Mouse Kidney." In Kidney Development, 165–71. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-851-1_15.

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Malaver-Ortega, Luis F., and Joseph Rosenbluh. "Immortalised Cas9-expressing Cell lines for Gene interrogation." In Methods in Molecular Biology, 91–97. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2301-5_5.

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MacDonald, Kelli P. A., Gillian R. Bushell, Perry F. Barlett, and Alan Mackay-Sim. "Neuronal and Glial Markers in Immortalized Olfactory Cell Lines." In Olfaction and Taste XI, 51. Tokyo: Springer Japan, 1994. http://dx.doi.org/10.1007/978-4-431-68355-1_20.

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Conference papers on the topic "Immortalized cell line"

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Tamo, Luca, Youssef Hibaoui, Sampada Kallol, Marco Alves, Christiane Albrecht, Anis Feki, Amiq Gazdhar, and Thomas Geiser. "Generation of an immortalized functional alveolar epithelial cell line originating from human induced pluripotent stem cells." In ERS International Congress 2016 abstracts. European Respiratory Society, 2016. http://dx.doi.org/10.1183/13993003.congress-2016.pa4030.

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Wang, G., H. Lou, J. Salit, P. L. Leopold, M. J. Thomas, J. Schymeinsky, K. Quast, S. Visvanathan, J. S. Fine, and R. G. Crystal. "An Immortalized Small Airway Basal Cell Line with Airway Region-Associated Diverse Differentiation Capacity." In American Thoracic Society 2019 International Conference, May 17-22, 2019 - Dallas, TX. American Thoracic Society, 2019. http://dx.doi.org/10.1164/ajrccm-conference.2019.199.1_meetingabstracts.a2831.

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Zhou, Yaling, Daniel Brooks, Sumana Dey, LeeAnn Higgins, Todd Markowski, Chad Hamilton, Robin Howard, Sorana Raiciulescu, Amy Skubitz, and John D. Andersen. "Abstract DPOC-003: DIFFERENTIAL PROTEIN EXPRESSION ANALYSIS OF SV40–IMMORTALIZED OVARIAN SURFACE, SV40–IMMORTALIZED FALLOPIAN TUBE AND OVARIAN CANCER SUBTYPE CELL LINE SECRETOMES BY ITRAQ®." In Abstracts: 11th Biennial Ovarian Cancer Research Symposium; September 12-13, 2016; Seattle, WA. American Association for Cancer Research, 2017. http://dx.doi.org/10.1158/1557-3265.ovcasymp16-dpoc-003.

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Oki, Akira, Shahnaz Begum, Mariana Brait, David Sidransky, and Mohammad Obaidul Hoque. "Abstract 811: Induction of stem-like cells with malignant properties by chronic exposure of immortalized normal human urothelial cell line to arsenic." In Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.am2015-811.

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Guiance-Varela, Carolina, Cristina Rodríguez-Pereira, Elena Fernandez-Burguera, Tamara Hermida Gómez, Noa Goyanes, Francisco J. Blanco, and joana magalhães. "FRI0516 CHONDROGENIC EFFECT OF KARTOGENIN ON AN IMMORTALIZED CELL LINE DERIVED FROM MESENCHYMAL STROMAL CELLS ISOLATED FROM HUMAN BONE MARROW." In Annual European Congress of Rheumatology, EULAR 2019, Madrid, 12–15 June 2019. BMJ Publishing Group Ltd and European League Against Rheumatism, 2019. http://dx.doi.org/10.1136/annrheumdis-2019-eular.5526.

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Michaelson, Jarett, Heejin Choi, Peter So, and Hayden Huang. "Mechanical Properties of Primary and Immortal Fibroblasts in Cell Bi-Layers." In ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80385.

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Immortalized cells are commonly used as analogs for primary cells in many cell mechanics, tissue engineering, and biochemical assays. However, it is not well-established whether immortal cell lines can mimic the behavior of primary cells in more physiological (three-dimensional) environments. For this project, we investigate the mechanical properties of primary cardiac fibroblasts (CFs) and 3T3 transformed fibroblasts when cultured in cell bi-layers by comparing the cells’ viscoelastic properties.
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Ceceris, Kyra D., Paul R. Ervin, and Theresa R. Cassino. "Comparison of signal transduction drug response markers in immortalized cell lines and primary patient specimens." In AACR International Conference: Molecular Diagnostics in Cancer Therapeutic Development– Sep 27-30, 2010; Denver, CO. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/diag-10-a31.

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Lawrence, Eliza E., Evita G. Weagel, Juan F. Mejia, Juan Arroyo, Shalee Killpack, Kim L. O'Neill, and Richard Robison. "Abstract 5160: Identifying TK1 localization in immortalized placental cell lines and in conditioned placental tissue." In Proceedings: AACR Annual Meeting 2018; April 14-18, 2018; Chicago, IL. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/1538-7445.am2018-5160.

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Alharbi, Zeyad, Md Miraj Kobad Chowdhury, Lynne Bingle, and Colin Bingle. "Air liquid interface (ALI)-grown immortalized human cell lines: a promising tractable model for human airway." In ERS International Congress 2020 abstracts. European Respiratory Society, 2020. http://dx.doi.org/10.1183/13993003.congress-2020.4327.

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Tsang, Hiu-Gwen, Lin Cui, Colin Farquharson, Brendan M. Corcoran, Kim M. Summers, and Vicky E. MacRae. "16 Investigating calcific aortic valve disease using novel immortalised sheep and rat valve interstitial cell lines." In 20th Scottish Cardiovascular, Forum Abstracts, February 4th 2017, University of Glasgow, UK. BMJ Publishing Group Ltd and British Cardiovascular Society, 1997. http://dx.doi.org/10.1136/heartjnl-2017-311433.16.

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Reports on the topic "Immortalized cell line"

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Henderson, R. F., J. J. Waide, and J. F. Lechner. Characterization of cloned cells from an immortalized fetal pulmonary type II cell line. Office of Scientific and Technical Information (OSTI), December 1995. http://dx.doi.org/10.2172/381391.

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Stanford, Janet, and Elaine Ostrander. Development of Immortalized Cell Lines from Hereditary Prostate Cancer Families. Fort Belvoir, VA: Defense Technical Information Center, September 2006. http://dx.doi.org/10.21236/ada483796.

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Hahn, William C. Development of Immortalized and Tumorigenic Prostate Cell Lines of Defined Genetic Constitution. Fort Belvoir, VA: Defense Technical Information Center, May 2002. http://dx.doi.org/10.21236/ada407292.

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Hahn, William C. Development of Immortalized and Tumorigenic Prostate Cell Lines of Defined Genetic Constitution. Fort Belvoir, VA: Defense Technical Information Center, May 2004. http://dx.doi.org/10.21236/ada426133.

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Hahn, William C. Development of Immortalized and Tumorigenic Prostate Cell Lines of Defined Genetic Constitution. Fort Belvoir, VA: Defense Technical Information Center, May 2003. http://dx.doi.org/10.21236/ada416489.

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Stampfer, Martha R. Regulation of hTERT Expression and Function in Newly Immortalized p53 (+) Human Mammary Epithelial Cell Lines. Fort Belvoir, VA: Defense Technical Information Center, June 2005. http://dx.doi.org/10.21236/ada440296.

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Stampfer, Martha R. Regulation of hTERT Expression and Function in Newly Immortalized p53(+) Human Mammary Epithelial Cell Lines. Fort Belvoir, VA: Defense Technical Information Center, June 2008. http://dx.doi.org/10.21236/ada491221.

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Stampfer, Martha R. Regulation of hTERT Expression and Function in Newly Immortalized p53(+) Human Mammary Epithelial Cell Lines. Fort Belvoir, VA: Defense Technical Information Center, June 2006. http://dx.doi.org/10.21236/ada457687.

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Stampfer, Martha R. Regulation of hTERT Expression and Function in Newly Immortalized p53(+) Human Mammary Epithelial Cell Lines. Fort Belvoir, VA: Defense Technical Information Center, June 2007. http://dx.doi.org/10.21236/ada472883.

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