Dissertations / Theses on the topic 'IBDV virus'
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Lüken, Caroline. "Untersuchungen zur Apoptoseinduktion des Virus der Infektiösen Bursitis (IBDV)." Doctoral thesis, Universitätsbibliothek Leipzig, 2009. http://nbn-resolving.de/urn:nbn:de:bsz:15-20090224-072016-6.
Full textXue, Chunyi, and 薛春宜. "Molecular characterization of infectious bursal disease virus (IBDV) receptor." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B31246187.
Full textWark, Kim Louise. "Expression and processing of infectious bursal disease virus proteins." Thesis, University of Hertfordshire, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323651.
Full textWong, Tsz-yeung, and 王子揚. "IBDV-mediated antiviral responses by TLR3 signaling pathways." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B41897201.
Full textWong, Tsz-yeung, and 王子揚. "Molecular characterization of IBDV-induced apoptosis in vitro using cDNA microarrays." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2005. http://hub.hku.hk/bib/B36375998.
Full textZenkina, Olga. "Etablierung eines Zellkultursystems zur Isolierung hochvirulenter Stämme des Virus der infektiösen Bursitis (IBDV)." Doctoral thesis, Universitätsbibliothek Leipzig, 2009. http://nbn-resolving.de/urn:nbn:de:bsz:15-20091019-093926-5.
Full textSachs, Katja. "Expression von Proteinen des Virus der infektiösen Bursitis (IBDV) mit Hilfe rekombinanter Influenzaviren." Doctoral thesis, Universitätsbibliothek Leipzig, 2005. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-34094.
Full textHot, David P. O. "Expression and characterisation of the polymerase (VP1) of Infectious Bursal Disease Virus (IBDV)." Thesis, University of Reading, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.297639.
Full textRudd, Matthew Francis, and mikewood@deakin edu au. "Virulence determinants of infectious bursal disease virus." Deakin University. School of Biological and Chemical Sciences, 2003. http://tux.lib.deakin.edu.au./adt-VDU/public/adt-VDU20050825.103742.
Full textRauf, Abdul. "PERSISTENCE, DISTRIBUTION AND IMMUNOPATHOGENESIS OF INFECTIOUS BURSAL DISEASE VIRUS IN CHICKENS." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1299612513.
Full textAricibasi, Merve [Verfasser]. "Comparison of the pathogenesis of infectious bursal disease virus (IBDV) in genetically different chickens after infection with virus strains of different virulence / Merve Aricibasi." Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2010. http://d-nb.info/1009589598/34.
Full textVan, Den Berg Thierry-Patrice R. "Base moléculaire de la variation antigénique du virus de la maladie de gumboro (IBDV): implications diagnostiques et vaccinales." Doctoral thesis, Universite Libre de Bruxelles, 1994. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/212684.
Full textDarteil, Raphaël. "Utilisation de l'herpèsvirus de la dinde (HVT) comme vecteur d'expression de la protéine immunogène VP2 du virus de la maladie de Gumboro (IBDV)." Lyon 1, 1995. http://www.theses.fr/1995LYO1T208.
Full textToskano, Hurtado Jennifer S. "Caracterización genómica y epidemiología molecular del virus de la Bursitis infecciosa en España." Doctoral thesis, Universitat Autònoma de Barcelona, 2016. http://hdl.handle.net/10803/393911.
Full textIn this thesis 3 studies were carried out in order to deepen the current epidemiological situation of the virus Infectious bursal disease (IBDV) in Spain. To get an overview of the current situation of IBD in Spain, the first study involved in the genetic characterization of the HVR of the VP2 protein of 967 isolates submitted during the years 1990 and 2015. Sequence analysis and phylogeny revealed that 21% strains in question were highly virulent strains. About 72% of the analyzed samples corresponded to the intermediate attenuated genotype and were highly related to the vaccine strains most commonly used in immunizing birds. Moreover, phylogeny showed new groups of strains that are not described in Spain .Therefore, an isolated group closely related to the classical Australian attenuated strain, and a new lineage called IBDV/Sp-var in the study which represents 1.8% of the viruses characterized. Regarding the results of the first study, a second study was done to characterize the molecular epidemiology of IBDV in Spain. In this study we considered 168 sequences of HVR of the VP2 obtained from GenBank with known origin and year, and 33 sequences derived from our previous study. Phylogenetic analysis using Bayesian inference confirmed the presence of 4 lines: classic (IBDV/cl), attenuated classical (IBDV/cl-at), very virulent (IBDV/vv) and the new lineage IBDV/Sp-var. The evolution of IBDV isolates was determined by defining the selective forces that were conducted during diversification. The Analysis of the selection pressure revealed a positive pressure within the attenuated lineage formed also by the intermediate virulence strain vaccine. The adaptive evolution showed a relationship between IBDV/at and IBDV/Sp-var lineages, in addition the comparison with a model of crystallographic structure of VP2 contained in the Data Bank Protein (DBP) determined that the codons 251 and 273 favored the emergence of the new strain IBDV/Sp-var. In order to identify the origin of the IBDV/vv and IBDV/Sp-var lineages and their expansion and dissemination a phylogeographic analysis was done. This analysis showed that the IBDV/vv strains were dispersed in Spain around 90s from Iran, and around the years 2002-2004 a large genetic diversity was distributed heterogeneously over time in the eastern region of the country. While the analysis of the lineage IBDV/Sp-var showed that its origin was around 1995 and was mainly distributed in the eastern region of Spain. Finally, the concordance of various molecular studies of IBD virus revealing that its virulence not only lies in the VP2, led us to propose the third study to characterize the complete genome of the virus. The segments A and B of 16 strains IBDV/vv detected in different regions of Spain and in different years were compared with highly virulent strains described in other countries, classical and attenuated strains and also with vaccine strains. The alignment and the phylogeny analysis showed that the different genotypes (IBDV/vv and IBDV/Cl-at) were grouped according to their virulence degree for the segment A and also for the segment B, and identifying some conserved residues in all strains of the genotype IBDV/vv and for both segments. However the IBDV/Spain/06/38 strain showed a mutated protein substitution VP3 when it is compared with the other strains. The recombination analysis confirmed by 6 of the 9 methods used in our research that the strain IBDV/Spain/06/38 presented a recombinant event.
Dias, Camila Cristina Almeida. "Comparação molecular de isolados patogênicos do vírus da doença infecciosa bursal do estado de Minas Gerais e construção de uma biblioteca subtrativa." Universidade Federal de Viçosa, 2007. http://locus.ufv.br/handle/123456789/5217.
Full textConselho Nacional de Desenvolvimento Científico e Tecnológico
Infectious bursal disease (IBD) has been the major preoccupation for the poultry industry, especially in the past decade with the emergency of high virulent strains. Mutations in the gene that code the VP2 viral protein of pathogenic strains and the amiss use of attenuate vaccines by few passages have been responsible for the springing of new outbreaks. The purpose of this work was to analyze phylogenetically two different isolates of IBDV in Minas Gerais State by the comparison of the nucleotide sequence of the gene that code VP2 viral capside protein. In order to study the pathogen-host interaction, a subtractive library was constructed from VERO cells infected by IBDV (8 hours post-infection) aiming the identification of differential gene expression during this interaction. For the phylogenic analysis of the isolates, fragments of 251 bp amplified by RT-PCR were cloned and sequenced. The comparison of the sequences revealed that these isolates have 98-100% identity with classical vaccinal IBDV strains indicating that these outbreaks might have been caused by the vaccinal virus. To construct the subtractive library, two cDNA populations were hibridizated: one derived by IBDV infected VERO cells and other by non infected VERO cells. The hibridizated products were amplified for the posterior cloning and evaluation of the differential express products. Understanding of the replication characteristics of the IBDV associated to the study of the virus infection effects in the gene expression of the host cell, shown in this work, will allow the elucidation of the mechanisms involved in the pathogen-host interaction contributing, therefore, for the development of methods more effectives in the control and prevention of the IBDV.
A doença infecciosa bursal (IBD) tem sido, há muitos anos, uma grande preocupação para a indústria avícola, especialmente na década passada devido a emergência de cepas virais hipervirulentas. Mutações no gene responsável pela codificação da proteína viral VP2 de linhagens patogênicas e a má utilização de vacinas atenuadas por poucas passagens têm sido responsáveis pelo aparecimento de novos surtos. A proposta deste trabalho foi analisar filogeneticamente dois diferentes isolados de IBDV de Minas Gerais por meio da comparação da seqüência nucleotídica do gene que codifica a proteína do capsídeo viral VP2. Em razão da necessidade de se estudar melhor a interação patógeno-hospedeiro, objetivou-se ainda a construção de uma biblioteca subtrativa a partir de células VERO infectadas pelo IBDV, 8 horas após a infecção, com a finalidade de identificar genes diferencialmente expressos durante essa interação vírus-célula hospedeira. Para a análise filogenética dos isolados, fragmentos de 251 pb amplificados por RT-PCR foram clonados e seqüenciados. A comparação das seqüências revelou que os isolados possuem identidade de 98-100% com linhagens clássicas vacinais de IBDV, indicando que os surtos analisados podem ter sido causados pelo vírus vacinal. Para a construção da biblioteca subtrativa, duas populações de cDNA foram hibridizadas: uma derivada de células VERO infectadas por IBDV e a outra de células VERO não infectadas. Os produtos hibridizados foram amplificados para posterior clonagem e avaliação dos produtos diferencialmente expressos. O conhecimento das características replicativas do IBDV associado ao estudo das conseqüências da infecção pelo vírus na expressão gênica da célula hospedeira, iniciado neste trabalho, permitirá a elucidação dos mecanismos envolvidos na interação patógeno-hospedeiro, contribuindo assim para o desenvolvimento de métodos mais eficazes no controle e prevenção da IBD.
Eldaghayes, Ibrahim Mohamed. "Use of chicken interleukin-18 as a vaccine adjuvant with a recombinant fowlpox virus fpIBD1, a subunit vaccine giving partial protection against IBDV." Thesis, University of Bristol, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.419211.
Full textGalloux, Marie. "Etude fonctionnelle et structurale d' un peptide impliqué dans le mécanisme d' entrée d' un virus non enveloppé." Paris 6, 2006. http://www.theses.fr/2006PA066035.
Full textGarriga, i. Rigau Damià. "Anàlisi estructural de partícules i proteïnes del virus de la bursitis infecciosa." Doctoral thesis, Universitat Pompeu Fabra, 2010. http://hdl.handle.net/10803/7196.
Full textThe infectious bursal disease, also known as Gumboro disease, is an avian pathology that affects broilers and chicks. In chicken farms, this virus, called IBDV, is responsible for high mortalities. The tridimensional structure determination at atomic resolution of this virus capsid protein, VP2, allowed us to characterize some of the elements that mediate the capsid assembly and stabilization. Furthermore, the residues implicated in the formation of inclusion bodies, that provide extra resistance to the virus in the extracellular stage, have been identified. Moreover, the tridimensional structure determination of the viral polymerase, VP1 protein, brought some light on the mechanisms involved in polymerization initiation and regulation of the activity mediated by VP3, the viral cycle coordination protein.
Jayaram, Jyothi. "Studies on the nucleocapsid protein of infectious bronchitis virus." Diss., Texas A&M University, 2003. http://hdl.handle.net/1969.1/2243.
Full textOberländer, Yvonne. "Herstellung und Charakterisierung von Serotyp 1-/Serotyp 2-Rekombinanten des Virus der Infektiösen Bursitis (IBVD)." Doctoral thesis, Universitätsbibliothek Leipzig, 2005. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-33967.
Full textMartini, Matheus Cavalheiro 1983. "Estudo experimental em camundongos e aves comerciais com isolado de pombo do vírus da bronquite infecciosa (IBV) = Experimental study in mice and poultry with isolated from pigeon infectious bronchitis virus (IBV)." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/316633.
Full textTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
Made available in DSpace on 2018-08-26T04:15:51Z (GMT). No. of bitstreams: 1 Martini_MatheusCavalheiro_D.pdf: 16763908 bytes, checksum: 65c4aed6451181f383a10560fce87e51 (MD5) Previous issue date: 2014
Resumo: O vírus da Bronquite Infecciosa (VBI), pertencente à família Coronaviridae, é um importante patógeno à sanidade e fatores econômicos da produção avícola no Brasil e no mundo. O VBI possui múltiplos sorotipos e o frequente surgimento de novas variantes é um dos principais problemas relacionados a este vírus. Este trabalho tem como objetivo a investigação experimental da patogênese de um isolado de pombo (Columba/Brazil/2007/Unicamp/67T), caracterizado molecularmente pelo gene S1 como VBI sorotipo Massachusetts, e seus efeitos in vivo, em galinhas e camundongos. O presente estudo foi dividido em duas partes, na primeira um grupo de aves "specific pathogen free" (SPF) foi inoculado pela via óculo-nasal com a amostra viral proveniente de pombo. Os animais, de um dia de vida, foram sacrificados nos dias 2, 4, 5, 7, 9, 11, 14, 21, 28, 35 e 42 dias pós-inoculação (dpi). Foram coletados suabes de traqueia, seio nasal e cloaca, além de órgãos como pulmão, íleo, pró-ventrículo (coletado entre 7 e 21 dpi), rim, tonsilas cecais (coletada a partir de 4dpi) e testículos (coletado a partir de 5 dpi). Sinais clínicos respiratórios como espirros, estertores, corrimento nasal, além de letargia, diarreia e perda de coordenação foram observados principalmente no 5dpi. A inibição da atividade ciliar ocorreu concomitantemente ao pico de sinais clínicos das aves. Foi analisado tropismo tecidual, através da quantidade de RNA viral detectado, pelo trato digestório. Os maiores títulos de RNA viral foram detectados na tonsila cecal, seguida pelo íleo (ambos no 5dpi) e cloaca (no 2dpi). Além disso, houve detecção de RNA viral no rim e trato respiratório, com maior título de RNA viral na traqueia. Os órgãos que apresentaram maiores danos teciduais através do exame histopatológico foram o rim, íleo e traqueia (todos no 5dpi). Por fim, as aves inoculadas com a amostra do VBI oriundo de pombo produziram anticorpos entre os dias 14 e 21dpi, detectados no soro destes animais através do ELISA. Na segunda parte do trabalho, a capacidade de replicação de diferentes variantes do VBI em camundongos foi avaliada. Para tanto, camundongos das linhagens Balb/C e A/J foram inoculados pela via nasal com duas amostras do sorotipo Massachussets (Mass) e com a variante brasileira (BR-I), e sacrificados no 3, 10 e 14 dpi. Não foram observados sinais clínicos nem lesões macroscópicas graves. O RNA viral foi detectado em todos os órgãos coletados, sendo os principais órgãos de replicação o seio nasal e pulmão (no 3dpi) para os camundongos da linhagem A/J e pulmão e duodeno (ambos no 3dpi) na linhagem de camundongos Balb/C, nos quais os títulos virais detectados foram mais altos. Pneumonia intersticial, edema e infiltrado mononuclear foram as principais alterações histopatológicas observadas no 3dpi em camundongos inoculados com as diferentes variantes. A presença da nucleoproteína viral, pela imunohistoquímica, foi detectada no duodeno, traqueia e pulmão de camundongos no 3dpi nas duas linhagens de camundongos. Os anticorpos contra o coronavírus aviário foram detectados somente no 3dpi. Assim, os resultados do presente estudo demonstraram que a variante Massachussets, com origem de pombo, causa a doença clínica em aves comerciais não vacinadas e pode replicar em modelo mamífero por um curto período de tempo, ressaltando a importância da vacinação e o papel potencial dos roedores como possível reservatório e carreador do vírus
Abstract: Infectious bronchitis virus (IBV) belonging to the family Coronaviridae is an important pathogen to sanity and economics of poultry production in Brazil and worldwide. The VBI has multiple serotypes and the frequent emergence of new variants is one of the main problems related to this virus. This work aims to experimentally investigate the pathogenesis of pigeon sample (Columba/Brazil/2007/Unicamp/67T), molecularly characterized by S1 gene as IBV Massachusetts serotype, and its effects in vivo in chickens and mice. This study was divided into two parts. In the first part, a group of birds "specific pathogen free" (SPF) was inoculated by oculo-nasal route with the viral sample from pigeon. The animals with one-day-old, were sacrificed on 2, 4, 5, 7, 9, 11, 14, 21, 28, 35 and 42 days post-inoculation (dpi). Tracheal swabs, nasal sinus and cloaca were collected, and organs such as lung, ileum, pro-ventricular (collected between 7 and 21dpi), kidney, caecal tonsils (collected from 4dpi) and testes (collected from 5 dpi). Clinical signs such as sneezing, rales, nasal discharge, lethargy, diarrhea, and loss of coordination were observed mainly in the 5dpi. Inhibition of ciliary activity occurred concomitantly with the peak of clinical signs of birds. Tissue tropism was analyzed by the amount of viral RNA detected by the gastrointestinal tract. The higher titers of viral RNA were detected in the cecal tonsil, followed by the ileum (both in 5dpi) and cloaca (in 2dpi). In addition, viral RNA was detected in the kidney and respiratory tract, with highest titer of viral RNA in the trachea. The organs that showed severe tissue damage by histopathology were the kidney, ileum and trachea (all in 5dpi). Finally, the birds inoculated with the sample originated from IBV Pigeon produced antibodies between 14 and 21dpi, detected in the serum of these animals by ELISA. In the second part, the replication capacity of different variants of IBV in mice was evaluated. For this, mice of strains BALB/C and A/J were inoculated intranasally with two strains of Massachusetts (Mass) serotype and the Brazilian variant (BR-I), and sacrificed at 3, 10 and 14 dpi. No clinical signs or severe macroscopic lesions were observed. The viral RNA was detected in all organs collected, higher tittles were detected on sinus and lung (in 3dpi) for mice of strain A/J and on lung and duodenum (both in 3dpi) in the line of Balb/C; in this line the viral titles were higher than the strain A/J. Interstitial pneumonia, edema and mononuclear cell infiltration were the main histopathological changes observed in 3dpi in inoculated mice with different variants. The presence of viral nucleoprotein, immunohistochemistry was detected in the duodenum, trachea and lungs of mice in 3dpi in both mice strains. Antibodies against avian coronaviruses have been detected only in 3dpi. Thus, the results of this study demonstrate that the Massachusetts variant, originating from pigeon, cause clinical disease in commercial poultry unvaccinated and can replicate in mammalian model for a short period of time, emphasizing the importance of vaccination and the potential role of rodents as possible reservoir and the carrier virus
Doutorado
Microbiologia
Doutora em Genética e Biologia Molecular
Quadros, Valter Leonardo de [Verfasser]. "Das Infektiöse Bronchitis Virus (IBV) : molekularbiologische Untersuchungen zur Diagnostik und zum Vorkommen sowie zur Pathogenität des Genotyps IBV QX in spezifisch pathogenfreien (SPF) Broilern / Valter Leonardo de Quadros." Berlin : Freie Universität Berlin, 2012. http://d-nb.info/102727577X/34.
Full textYoun, Soonjeon. "In vitro assembly of an infectious cDNA clone of infectious bronchitis virus and its application as a gene transfer vector." Diss., Texas A&M University, 2003. http://hdl.handle.net/1969.1/1311.
Full textSandri, Thaisa Lucas. "Vírus da bronquite infecciosa das galinhas (IBV): distribuição, diversidade molecular e genealogia a partir de amostras de múltiplos órgãos de diversos tipos de criação do plantel avícola brasileiro." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-21122010-105658/.
Full textInfectious bronchitis (IB) is a highly contagious disease of poultry caused by multiple geno/serotypes of avian infectious bronchitis virus (IBV), a group 3 coronavirus. Though classically associated to the respiratory tract, IBV strains also have been described which harbor tropism for the kidneys and the reproductive and enteric tracts, and might be detected in multiple tissues and can also affect birds of all ages. This survey aimed to assess the frequency of in multiple organs and enteric content samples from grandparents, breeders, layers and broilers, to genotype the IBV strains detected and to study the molecular diversity amongst Brazilian IBV strains. A total of 844 pools of multiple organs and enteric contents from 200 flocks of grandparents, breeders, layers and broilers from the Southern, Southeastern, Central-Western and Northeastern Brazilian regions collected between 2007 and 2009 was screened for the presence of IBV with an RT-PCR target to the 3 untranslated region (UTR). The sampled birds presented symptoms compatible with IB. All IBV strains detected were then typed using an RT-PCR target to the spike gene of the virus. Nineteen strains type as variants were submitted to partial sequencing of the S1 coding region and genealogic analysis. Regarding the organs and enteric content pools, 45.50% were positive for the presence of IBV, from which 84.63% were variant and 9.89% Massachusetts. Taking into account the flocks, 73.50% were positive for IBV, being 77.55% variants and 6.12% Massachusetts. Genealogic analysis revealed four viral lineages, all grouped in an exclusive Brazilian genotype cluster. This results shown that IBV is widespread in all Brazilian poultry regions, with a massive predominance of non-Massachusetts genotypes and a high molecular diversity, which must be taken into account in order to develop preventive measures against IB.
Lopez, Juan Carlos. "The effect of environmental stressors on the immune response to avian infectious bronchitis virus." Lincoln University, 2006. http://hdl.handle.net/10182/643.
Full textStephan, Thomas Min [Verfasser], Silke [Akademischer Betreuer] Rautenschlein, Georg [Akademischer Betreuer] Herrler, and Egbert [Akademischer Betreuer] Mundt. "Molecular characterization of the spike protein of an avian infectious bronchitis virus (IBV) with cell culture tropism and identification of the underlying determinants / Thomas Min Stephan ; Silke Rautenschlein, Georg Herrler, Egbert Mundt." Hannover : Stiftung Tierärztliche Hochschule Hannover, 2019. http://d-nb.info/1202774873/34.
Full textVukea, Phillia Rixongile. "Characterisation of infectious bursal disease virus (IBDV) polyprotein processing." Thesis, 2011. http://hdl.handle.net/10413/10356.
Full textThesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2011.
Lüken, Caroline. "Untersuchungen zur Apoptoseinduktion des Virus der Infektiösen Bursitis (IBDV)." Doctoral thesis, 2008. https://ul.qucosa.de/id/qucosa%3A10767.
Full textLüken, Caroline [Verfasser]. "Untersuchungen zur Apoptoseinduktion des Virus der infektiösen Bursitis (IBDV) / eingereicht von Caroline Lüken." 2009. http://d-nb.info/993165133/34.
Full textBanda, Alejandro. "Characterization of field strains of infectious bursal disease virus (IBDV) using molecular techniques." 2002. http://purl.galileo.usg.edu/uga%5Fetd/banda%5Falejandro%5F200208%5Fphd.
Full textPetkov, Daniel Iordanov. "Characterization of the chicken antibody response against infectious bursal disease virus (IBDV), IBDV impact on B-cells and full-length genomic sequencing followed by antigenic and phylogenetic analysis of IBDV strains with different pathogenicities." 2005. http://purl.galileo.usg.edu/uga%5Fetd/petkov%5Fdaniel%5Fi%5F200508%5Fphd.
Full textDirected by Mark Jackwood. Includes articles submitted to Veterinary immunology and immunopathology, Virus genes, and Virus research. Includes bibliographical references.
Hamoud, Mohamed Mamdouh. "Studies on infectious bursal disease virus (IBDV) dsRNA extracted from formalin fixed paraffin embedded tissue." 2006. http://purl.galileo.usg.edu/uga%5Fetd/hamoud%5Fmohamed%5Fm%5F200608%5Fphd.
Full textYu-Ting, Hsu, and 許郁停. "Comparison of Immunprotectivity Provided by the Conventional and the Baculovirus Expressied VP2 Subunit Protein of Infectious Bursal Disease Virus (IBDV) as Inactivated IBDV Vaccine Antigen in Chickens." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/41798048815901870805.
Full text國立屏東科技大學
生物科技研究所
91
Abstract Infectious bursal disease virus (IBDV) is a immunosuppressive infectious disease in causing necrotic of B-lymphoblast in chickens at 3 to 6 weeks of age. Viral protein (VP) 2 and 3 are the mainly important 2 structural proteins of IBDV. The VP2 of IBDV contains the antigenic epitopes in inducing the neutralizing antibody. The whole length of VP2 gene, approximately 1.5 Kb nucleotides, of a recently local isolate (V263/TW02) IBDV being verified with good cross protectivity against several local isolates was amplified with reverse transcriptase-polymerase chain reaction , sequenced and phylogenic analysed of the nucleotide as a very virulent IBDV (vvIBDV) with some point mutation. The gene was then cloned in the vector of the baculovirus expression system for producing the most protective VP2 subunit protein as antigen for IBDV V263/TW02-VP2 subunit vaccine preparation. A3(48 kDa)and C2(21 kDa)2 subunit proteins was found expressied in the system. The V263/TW02-VP2-C2 subunit vaccine was then used to immunized the specific-pathogen-free chickens for comparison of the protectivity with the conventional inactivated V263/TW02 IBDV vaccine against the homologous IBDV challenge. The V263/TW02-VP2 subunit vaccine was found inducing a much higher titer of enzyme-linked immunosorbent assay antibody response but less protective than the inactived V263/TW02 vaccine did. The V263/TW02-VP2-C2 subunit protein might also be good for preparing serological diagnostic kits. The immunogenicity of V263/TW02-VP2-A3 subunit protein need to be further evaluated.
Liao, Jung-Yi, and 廖君儀. "Enhancement of the production and protection efficiency of IBDV virus like particle expressed in E. coli." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/ug2y44.
Full text國立中興大學
微生物暨公共衛生學研究所
101
The viral particle of infectious bursal disease virus (IBDV) is composed of VP2 and VP3 on the outside and inside, respectively. During the formation of viral particle, a polyprotein VP243 was first produced and self-cleaved into precursor VP2 (pVP2) and VP3 by VP4. The researchers had shown that pVP2 C-terminal was further cut by VP4 and partially by host cell enzyme, and finally by VP2-itself to form mature VP2 and finally produce viral particle. However, the role of VP4 in the final steps of viral partical formation is still not clear. The objectives of this study are (1) to investigate the requirement of VP4 in the formation of virus like particle (VLP) after that pVP2 and VP3 have been already produced; (2) to construct various recombinant plasmids to investigate the production of VLP and subviral particle (SVP) in E. coli and evaluate the protection efficacy of theses recombinant proteins against very virulent IBDV (vvIBDV) challenge. Five plasmids were constructed, including (1) pTri VP2-452 (encoding for the first 452 amino acids of VP2, VP2-452, the pVP2 after cutting by host cell enzyme); (2) pET VP243 (encoding for VP243); (3) pET 512/VP3/452 (encoding for the full length of pVP2 (VP2-512), VP3 and VP2-452); (4) pET 487/VP3/452 (encoding for the pVP2 after the last cut by VP4 (VP2-487), VP3 and VP2-452); (5) pET VP243/452 (encoding for VP243 and VP2-452). The plasmids were transformed into E. coli and the expressed virus like particles were separated by sucrose gradient centrifugation and analyzed by ELISA and electron microscopy. The pET VP243 and pET VP243/452, but not the plasmids without VP4 gene, had higher 70-nm-VLP production efficiency. After challenge with 2×102 EID50 vvIBDV, the proteins expressed by pTri VP2, pET VP243 and pET VP243/452 could provide more than 80 % protection of chickens against vvIBDV. However, proteins expressed by pET 512/VP3/452 and pET 487/VP3/452 could only provide protection less than 60%. When ISA 71was used as adjuvant, the anti-IBDV antibody titer was significantly higher than that when Freund’s adjuvant was used. The severe inflammations were observed in the injection sites of proteins with ISA 71. The results indicated that VP4 play an important role in the final steps of VP2 maturation and the formation of VLP. Moreover, pET VP243 and pET VP243/452 can not only produce VLP in E. coli but also provide efficacious protection of chicks against vvIBDV.
Sachs, Katja [Verfasser]. "Expression von Proteinen des Virus der infektiösen Bursitis (IBDV) mit Hilfe rekombinanter Influenzaviren / eingereicht von Katja Sachs." 2005. http://d-nb.info/977714462/34.
Full textZenkina, Olga [Verfasser]. "Etablierung eines Zellkultursystems zur Isolierung hochvirulenter Stämme des Virus der infektiösen Bursitis (IBDV) / eingereicht von Olga Zenkina." 2009. http://d-nb.info/998842710/34.
Full textLee, Meng-Shiou, and 李孟修. "Functional Characterization of VPX protein and Polyprotein of Avian Infectious Bursal Disease Virus (IBDV) on Virus-like Particles Assembly and Proteolytical Cleavage in Insect Cells." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/74329720715264474129.
Full text國立中興大學
生物科技學研究所
92
VP2 is a major structural protein of infectious bursal disease virus(IBDV). It has been demonstrated as the major host-protective immunogen of IBDV and contains the antigenic regions responsible for eliciting the virus neutralizing antibodies in the host. In this study, the precursor protein (VPX) of infectious bursal disease virus (IBDV) host immunogen VP2 protein was expressed in insect cells, including Sf9 and Hi-5 cells, to examine its regenerated particle types and the immunogenicity induced by those particles. Since the expressed protein, VPXH, was engineered a His-tag, consisting of six histidine residues, at their C-terminal end. When expressed in Hi-5 cells, rVPXH was efficiently processed at its C-terminus by cellular proteases to yield VP2-like proteins whose molecular weight was similar to that of VP2. However, proteolytical processing of VPXH in Sf9 cells was hampered. The expressed rVPXH was purified using immobilized metal-ion affinity chromatography (IMAC). Under TEM observation of Ni-NTA purified VPXH, at least three architectures of particles were observed, including the tubular structure and two of spherical structure of isometric particle structure and a new one of icosahedral particles, with a size of approximately 20-25nm and 30-35 nm in diameter, respectively. After separation of rVPXH formatted particles, chromatographic results indicate that the expressed rVPXH protein and very few of VP2-like protein formed isometric particle structure and very few of twisted tubular structure, as well as icosahedral particles formed by the degraded products of rVPXH protein, VP2-like protein. Finally, we also demonstrated that when susceptible chickens were vaccinated with the IMAC-purified rVPXH protein (40 g/bird), virus-neutralizing antibodies were induced. This indicated that those particles are highly immunogenic. Based on our results, we found that Hi-5 harbors excellent ability of proteolytic cleavage of VPX. Therefore, this study our effort also to investigate the proteolytic processing of IBDV polyprotein in insect cell. When IBDV polyprotein was expressed in insect cells, higher productivity of IBDV VLP was observed in Hi-5 cells. Moreover, the accumulation of matured VP2 and VLPs assembly was exhibited rather efficiently than in Sf9. In addition to IBDV-like particles assembled in Hi-5 cells, some of particulated subviral particles with 23 nm in diameter were also assembled. Chromatographic results show that the IBDV subviral particle was formed by VP2 protein. When a higher multiplicity of infection(MOI) strategy was used, accumulation of VP2 protein is more significantly. The excess VP2 protein resulted in formation of subviral particles. However, at low MOI, the relative productivity of IBDV VLP and subviral particles increased in batch culture. Our results demonstrate that Hi5, harboring excellent ability of proteolytic cleavage of recombinant protein, the efficiency of IBDV-like particles production is superior to Sf9 culture. These finding therefore may provide a methodological improvement using Hi-5 cells for optimal production of IBDV VLP as an effective IBDV vaccine against infectious bursal disease or as for crystallization to study IBDV structure.
Oberländer, Yvonne [Verfasser]. "Herstellung und Charakterisierung von Serotyp 1-,Serotyp 2-Rekombinanten des Virus der Infektiösen Bursitis (IBDV) / eingereicht von Yvonne Oberländer." 2004. http://d-nb.info/976005913/34.
Full textOberländer, Yvonne. "Herstellung und Charakterisierung von Serotyp 1-/Serotyp 2-Rekombinanten des Virus der Infektiösen Bursitis (IBVD)." Doctoral thesis, 2004. https://ul.qucosa.de/id/qucosa%3A10602.
Full textChiu, Tsung-Ting, and 邱宗鼎. "Polymorphisms of Infectious Bronchitis Virus (IBV) S1 Sequence following Passage in Chicken Embryonated Eggs and Investigation of Prevalent IBV Strains in Taiwan." Thesis, 2019. http://ndltd.ncl.edu.tw/cgi-bin/gs32/gsweb.cgi/login?o=dnclcdr&s=id=%22107NCHU5773006%22.&searchmode=basic.
Full text國立中興大學
微生物暨公共衛生學研究所
107
Infectious bronchitis virus (IBV) causes infectious bronchitis (IB) in chicken and leads to severe economic loss. Since the poor cross-protection among different serotypes of IBVs, co-administration of two different serotypes of attenuated IBV vaccines are regularly applied in the poultry industry. Recombination between vaccine strains may alter the antigenicity of vaccine strain. Moreover, vaccine strains may serve as heterologous RNA donors and result in the appearance of new IBV serotypes. The objectives of this study were to investigate the prevalent IBV strains in the field by detection of IB viruses obtained from poultry slaughter house, and also to assess the risk of current IB vaccination strategy by analyzing S1 polymorphisms of IBVs collected from embryonated chicken eggs inoculated with single or dual IBV strains through one or two passages. Primer sets were designed to distinguish IBV strains among various vaccine and field virulent strains by reverse transcription polymerase chain reaction (RT-PCR). Among one hundred IBV positive fecal samples collected from slaughter house, sixty samples contained TW-I and fifteen of these samples contained vaccine strains as well. The result demonstrated that the two virus strains could replicate in chicken at the same time. Therefore, single IBV strains (single groups) or two IBV strains (dual groups) were passaged in embryonated chicken eggs for simulating the circumstances of single or dual viruses replicating in chickens in the field. The vaccine strains of Ma5, H120, and 4/91 as well as field strain TW100 were used in this study. Single groups included all four strains and dual groups included 5 groups which were MH, M4, H4, HT, and 4T. The entire S1 genes of IBVs were amplified with strain specific primers. The intertypic recombinations were not identified in all inspected S1 sequences. However, the average single nucleotide polymorphisms (SNPs) counts per sequence from dual groups were higher than from single groups. By further inspection of secondary peaks within original sequencing chromatograms, the mechanism of changes in SNPs were categorized into selection effect and mutation effect. The results showed that both selection and mutation effects were facilitated in eggs inoculated with dual IBV strains. In conclusion, the prevalent IBV strain is TW-I and replication of dual strains within same chicken embryo could facilitate both selection and mutation effect.