Academic literature on the topic 'Hypotonic shock'

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Journal articles on the topic "Hypotonic shock"

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Dinh, Xuan Tu, Huynh Thi Diem Suong Le, and Minh Ly Nguyen. "The chromosome numbers of Panax vietnamensis Ha et Grushv." Can Tho University Journal of Science 14, CBA (2022): 86–90. http://dx.doi.org/10.22144/ctu.jen.2022.033.

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The somatic chromosome number of Panax vietnamensis Ha et Grushv. was determined to be 2n = 24, based on the hypotonic shock method by potassium chloride solution. In this study, we investigated the effect of potassium chloride and colchicine solutions on chromosome dispersion of Panax vietnamensis at different concentrations. The treatment using 0.2% KCl solution in 45 minutes combined with 0.05% colchicine solution in 2 hours subsequently resulted in proper hypotonia. The result showed that chromosomes were evenly dispersed. The hypotonic shock method seemed to be effective in equally distri
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Beck, J. S., S. Breton, G. Giebisch, and R. Laprade. "Potassium conductance regulation by pH during volume regulation in rabbit proximal convoluted tubules." American Journal of Physiology-Renal Physiology 263, no. 3 (1992): F453—F458. http://dx.doi.org/10.1152/ajprenal.1992.263.3.f453.

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When rabbit proximal convoluted tubules were microperfused in the presence of bicarbonate, a 90 mosmol hypotonic shock hyperpolarized the basolateral membrane by 5.5 +/- 1.4 mV, increased basolateral potassium selectivity (tK) from 0.30 +/- 0.02 to 0.45 +/- 0.02, and reduced the basolateral membrane resistance from 4,887 +/- 821 to 2,836 +/- 602 omega.cm. These data show that the hypotonic shock increased absolute basolateral potassium conductance. The same hypotonic shock elevated intracellular pH from 7.18 +/- 0.04 to 7.31 +/- 0.04. When bath pH was increased by 0.2 pH units (by reduction of
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Marshall, W. S., S. E. Bryson, and T. Luby. "Control of epithelial Cl(−) secretion by basolateral osmolality in the euryhaline teleost Fundulus heteroclitus." Journal of Experimental Biology 203, no. 12 (2000): 1897–905. http://dx.doi.org/10.1242/jeb.203.12.1897.

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Euryhaline teleost fish adapt rapidly to salinity change and reduce their rate of ion secretion on entry to fresh water. Killifish (Fundulus heteroclitus) transferred from full-strength sea water to fresh water showed large reductions in plasma [Na(+)] and osmolality at 6 h which were corrected by 24 h. To mimic this in vitro, a hypotonic shock of 20–70 mosmol kg(−)(1) was applied on the basolateral side of opercular epithelia. This hypotonic shock reversibly reduced the short-circuit current (I(sc), equivalent to the rate of secretion of Cl(−)) in a dose-dependent fashion, with a 40 mosmol kg
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Bear, C. E. "A nonselective cation channel in rat liver cells is activated by membrane stretch." American Journal of Physiology-Cell Physiology 258, no. 3 (1990): C421—C428. http://dx.doi.org/10.1152/ajpcell.1990.258.3.c421.

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A 16-pS channel was studied using patch-clamp electrophysiology in freshly dissociated rat liver cells and rat hepatoma cells. The channel was found to be cation selective and permeable to Na+, K+, and Ca2+. Its gating was unaffected by addition of the calcium ionophore A23187 (5 microM) in the presence of extracellular Ca2+ (2 mM). Ca2+ channel blockers, nifedipine, verapamil, and lanthanum, failed to inhibit the channel. The channel was activated by stretch, applied as suction to the interior of the patch pipette, and by cell swelling, induced by hypotonic shock or organic solute uptake (10
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Fujii, Shuhei, and Johan A. Hellebust. "Release of intracellular glycerol and pore formation in Dunaliella tertiolecta exposed to hypotonic stress." Canadian Journal of Botany 70, no. 7 (1992): 1313–18. http://dx.doi.org/10.1139/b92-164.

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When Dunaliella tertiolecta cells, previously cultured in a 0.5 M NaCl medium, were resuspended in a 0.25 NaCl medium, about 50% of the intracellular glycerol was lost within 2 min. A corresponding amount of glycerol appeared in the medium, while other organic solutes, such as amino acids and sugars, were not detected. These results indicate that intracellular glycerol is rapidly released without significant concomitant cell damage. Rubidum, in the case of rubidium-loaded cells, was also rapidly released to the medium in response to hypotonic shock. Gramicidin, dimers of which form stable memb
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Sawaied, Alaa, Bat-El Levy, Eden Arazi, Eitan Lunenfeld, Qinghua Shi, and Mahmoud Huleihel. "Follicle-Stimulating Hormone and Testosterone Play a Role in the Regulation of Sertoli Cell Functions Following Germ Cell Depletion In Vitro." International Journal of Molecular Sciences 26, no. 6 (2025): 2702. https://doi.org/10.3390/ijms26062702.

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Spermatogenesis is a process of self-renewal of spermatogonial stem cells and their proliferation and differentiation to generate mature sperm. This process involves interactions between testicular somatic (mainly Sertoli cells) and spermatogonial cells at their different stages of development. The functionality of Sertoli cells is regulated by hormones and testicular autocrine/paracrine factors. In this study, we investigated the effects of follicle-stimulating hormone (FSH) and testosterone addition on Sertoli cell cultures that undergo hypotonic shock, with a primary focus on Sertoli cell a
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Macri, P., S. Breton, J. S. Beck, J. Cardinal, and R. Laprade. "Basolateral K+, Cl-, and HCO3- conductances and cell volume regulation in rabbit PCT." American Journal of Physiology-Renal Physiology 264, no. 2 (1993): F365—F376. http://dx.doi.org/10.1152/ajprenal.1993.264.2.f365.

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The relationship between changes in cellular volume, intracellular pH (pHi), basolateral membrane potential (VBL), and membrane partial basolateral conductances to K+ (tK) and Cl- (tCl) and mediated by the Na-HCO3 cotransporter (tNaHCO3) was determined in the collapsed proximal convoluted tubule (PCT) submitted to a 125-mosmol/kg hypotonic shock. The shock that produces a rapid swelling followed by partial volume regulation was accompanied by a rapid and transient VBL hyperpolarization of 10.0 +/- 1.5 mV and a second gradual hyperpolarization of 5.0 +/- 0.7 mV with respect to a control value o
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Galietta, L. J., S. Falzoni, F. Di Virgilio, G. Romeo, and O. Zegarra-Moran. "Characterization of volume-sensitive taurine- and Cl(-)-permeable channels." American Journal of Physiology-Cell Physiology 273, no. 1 (1997): C57—C66. http://dx.doi.org/10.1152/ajpcell.1997.273.1.c57.

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Volume-sensitive Cl- channels [ICl(vol)] were studied using taurine efflux and patch-clamp experiments in 9HTEo- human tracheal cells. Cells were stimulated with the Ca(2+)- elevating agents ATP and ionomycin in isotonic medium or in hypotonic solutions. ATP (100 microM) or ionomycin (1 microM) and hypotonic shock produced a synergic effect. Indeed, the resulting taurine efflux was much higher than the sum of the single effects elicited by ATP, ionomycin, or hypotonic medium. The taurine release elicited by hypotonic shock and the potentiation by ATP and ionomycin were markedly inhibited by us
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Smets, Ilse, Marcel Ameloot, Paul Steels, and Willy Van Driessche. "Loss of cell volume regulation during metabolic inhibition in renal epithelial cells (A6): role of intracellular pH." American Journal of Physiology-Cell Physiology 283, no. 2 (2002): C535—C544. http://dx.doi.org/10.1152/ajpcell.00371.2001.

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In renal ischemia, tubular obstruction induced by swelling of epithelial cells might be an important mechanism for reduction of the glomerular filtration rate. We investigated ischemic cell swelling by examining volume regulation of A6 cells during metabolic inhibition (MI) induced by cyanide and 2-deoxyglucose. Changes in cell volume were monitored by recording cell thickness ( T c). Intracellular pH (pHc) measurements were performed with the pH-sensitive probe 5-chloromethyl-fluoresceine diacetate. T c measurements showed that MI increases cell volume. Cell swelling during MI is proportional
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Shpakova, Natalia, and Natalia Orlova. "About the Mechanism of Mammalian Erythrocytes Osmotic Stability." Problems of Cryobiology and Cryomedicine 30, no. 4 (2020): 331–42. http://dx.doi.org/10.15407/cryo30.04.331.

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The peculiarities of the effect of hypertonic shock and hypotonic stress on erythrocytes of different species of mammals (human, bull, horse, rabbit, dog, rat) have been investigated. Based on the results of correlation analysis (using the Spearman’s rank correlation coefficient), the relationship between osmotic sensitivity of mammalian erythrocytes and the well-known structural and functional characteristics of these cells was assessed. The paper presents and analyzes the significant relationships. Under hypotonic stress of mammalian erythrocytes, the values of the threshold concentration of
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Dissertations / Theses on the topic "Hypotonic shock"

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GROPPI, SILVIA. "Glucose and osmotic stress-dependent calcium signalling in saccharomyces cerevisiae: evidences for novel transporter systems and calcineurin involvement." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2012. http://hdl.handle.net/10281/29495.

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The major goal of this work is to understand function and regulation mechanisms of calcium transport systems in Saccharomyces cerevisiae, in response to nutrients and hypotonic shock. Calcium represents, in fact, one of the most important second messengers in all eukaryotic cells, and particularly in budding yeast, where it plays essential roles in regulating many fundamental cellular processes, such as cell cycle, mating, sensing of glucose and glucose starvation, resistance to salt stress and cell survival. Yeast cells actively maintain cytosolic free Ca2+ concentration at extremely low
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Umam, Khotibul, and 郭迪曼. "The potential osmoprotective roles of branchial heat shock proteins in milkfish upon hypotonic challenge." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/47424911364428395954.

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碩士<br>國立中興大學<br>生命科學院碩士在職專班<br>104<br>The present study investigated the HSPs expression induced by hypotonic stress in gills of euryhaline milkfish. Since heat shock response is a predominant cellular stress response, two of its major components, heat shock protein 70 (HSP70) and 90 were examined in this study. Four hsp genes were first identified from the transcriptome database, divided into inducible (hsp under stress) and constitutive (hsp unstressed) forms. The alignment and phylogenetic analysis of the sequences revealed the four hsps (mfhsp) groups of milkfish, including mfhsp70, mfhsc7
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Book chapters on the topic "Hypotonic shock"

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Galietta, L. J. V., V. Barone, D. C. Gruenert, and G. Romeo. "A Chloride Conductance Evoked by Hypotonic Shock in Epithelial Cells." In Advances in Experimental Medicine and Biology. Springer US, 1991. http://dx.doi.org/10.1007/978-1-4684-5934-0_29.

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Mastrocola, Teresa, and Michela Rugolo. "The Response of Chloride Transport to Cyclic AMP, Calcium and Hypotonic Shock in Normal and Cystic Fibrosis Fibroblasts." In Advances in Experimental Medicine and Biology. Springer US, 1991. http://dx.doi.org/10.1007/978-1-4684-5934-0_43.

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Singh, Amandeep, and Aayush Chawla. "The Place of Crystalloids." In Rational Use of Intravenous Fluids in Critically Ill Patients. Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-42205-8_9.

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AbstractCrystalloids are commonly used in medicine as solutions containing electrolytes dissolved in water, with or without glucose. They can be used as maintenance, replacement, or resuscitation fluids, but should be administered with caution. This chapter provides an overview of basic definitions, terminology, and concepts regarding crystalloids, including their categorization by tonicity, their balanced or unbalanced nature, and the importance of strong ion difference (SID). Improper administration of crystalloids can lead to morbidity, particularly hyperchloremic metabolic acidosis (HMA) a
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Conference papers on the topic "Hypotonic shock"

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Dagenais, Andre, Marie-Claude Tessier, Sabina Tatur, Ryszard Grygorczyk, and Yves Berthiaume. "Hypotonic Shock Increases Na+ Current Via A Cl-And Ca++ Dependent Mechanism In Alveolar Epithelial Cells." In American Thoracic Society 2012 International Conference, May 18-23, 2012 • San Francisco, California. American Thoracic Society, 2012. http://dx.doi.org/10.1164/ajrccm-conference.2012.185.1_meetingabstracts.a3531.

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Holme, S., W. A. Heaton, and P. Hartman. "INDIUM-111 PLATELET SURVIVAL STUDIES ON PLATELET CONCENTRATES (PCVSTORED FOR UP TO 14 DAYS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644690.

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The effect of extended storage on platelet in vitro and in vivo viability was investigated to determine the reliability and efficacy of Indium-111-oxine radiolabeling of long-term stored platelets. The fitness of various mathematical models for measurement of platelet survival and recovery was also evaluated.33 CPDA-1 PC were prepared and stored for various periods of time (see below) according to standard blood bank methods. The volume of the PC was adjusted to 65-75 mL to prevent pH fall with extended storage. After storage, samples were taken for in vitro assays and for radiolabeling witn I
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