Academic literature on the topic 'Hyperdermic'

During surveys of insect pathogenic fungi (IPF) in Thailand, fungi associated with scale insects and plants were found to represent five new species of the genus Ascopolyporus in Cordycipitaceae. Their macroscopic features resembled both Hyperdermium and Ascopolyporus. Morphological comparisons with the type and known Ascopolyporus and Hyperdermium species and phylogenetic evidence from a multigene dataset support the appointment of a new species of Ascopolyporus. Moreover, the data also revealed that the type species of Hyperdermium, H. caulium, is nested within Ascopolyporus, suggesting that Hyperdermium is congeneric with Ascopolyporus. The specimens investigated here differ from other Ascopolyporus species by phenotypic characters including size and color of stromata. Phylogenetic analyses of combined LSU, TEF1, RPB1 and RPB2 sequences strongly support the notion that these strains are distinct from known species of Ascopolyporus, and are proposed as Ascopolyporus albus, A. galloides, A. griseoperitheciatus, A. khaoyaiensis and A. purpuratus. Neohyperdermium gen. nov. is introduced for other species originally assigned to Hyperdermium and Cordyceps occurring on scale insects and host plants as epiphytes, accommodating two new combinations of Hyperdermium pulvinatum and Cordyceps piperis.

Colletotrichum acutatum J. H. Simmonds was isolated from diseased leaves of ornamental Kalmia latifolia L. (mountain laurel) cvs. Carousel and Peppermint on plants imported from the United States to Edinburgh, Scotland, in December 1999. Symptoms included sunken, desiccated, darkened necrotic areas, primarily at the leaf tip. Necrotic areas advanced toward the leaf base and were bordered by purple/red pigmentation. Isolations were made from salmon colored conidiomata that developed on abaxial leaf surfaces following incubation in a humidity box at 25°C for 7 to 10 days. White aerial mycelia, becoming gray to grayish beige, and producing salmon to orange colored conidial masses, formed on potato dextrose agar after 10 to 14 days. Conidia were hyaline, aseptate, fusiform to slightly irregular, and measured 13.4 to 13.8 × 4.3 to 4.9 μm. Both morphological and conidial characteristics were consistent with the description of C. acutatum (2). The identity of isolates was further verified by positive plate-trapped antigen ELISA of conidial preparations using a species-specific monoclonal antibody (1). Pathogenicity was assessed by inoculating the adaxial surface of healthy leaves of both cultivars of the imported plants with colonized agar disks and a range of spore suspensions (30, 300, and 3,000 spores delivered in 30 μl volumes) from test fungal isolates and a confirmed laboratory strain (three replicates per treatment). To ensure inoculum uptake, two 5 mm2 areas of cuticle on either side of the mid-rib of each leaf were lightly scratched with a sterile hyperdermic needle prior to inoculation. Inoculated leaves were incubated in a humidity box at 25°C for up to 3 weeks. Symptom development was progressive but relatively slow on both cultivars. The relatively slow development on artificially infected leaf material may be partly attributable to residual fungicide treatment as prescribed by the Scottish Plant Health Service at the time of planting out. Symptoms produced on fruits (apple, banana, and strawberry), inoculated with both test and laboratory strains of the fungus, were identical. Symptoms did not occur on control leaves or fruits inoculated with sterile distilled water or uninoculated agar disks. Koch's postulates were confirmed by consistently reisolating isolates with morphological and immunological characteristics identical to the fungal isolate used to initially inoculate test material. Over the same period, additional symptoms, identical to those originally described at the time of interception, continued to develop on leaf tips of both Kalmia cultivars. Additional isolations from this material were characterized as C. acutatum. Identification of representative isolates was confirmed by CABI Bioscience, Egham, UK, where a reference culture (accession number IMI 384569) has been deposited. As advanced symptoms were observed immediately on arrival of this consignment in the UK, original infection is thought to have occurred prior to importation. This is the first report of C. acutatum infecting K. latifolia. References: (1) I. Barker et al. 1994. Pages 179-182 in: Assays for Plant Pathogenic Fungi: Identification, Detection and Quantification. A. Schots, F. M. Dewey, and R. Oliver, eds. CABI International, Wallingford, UK. (2) B. J. Dyko and J. E. M. Mordue. CMI Descriptions of Pathogenic Fungi and Bacteria. No. 630, 1979.

ABSTRACT Bacillus strain UTK D1-0055 was isolated from a laboratory environment and appeared to have antilisterial activity. The genome was sequenced, the strain was identified as Bacillus altitudinis, and a high-quality complete annotated genome was produced. The taxonomy was evaluated for this and related Bacillus species (B. aerophilus, B. pumilus, B. safensis, B. stratosphericus, and B. xiamenensis) because the taxonomy is unclear and contains errors in public databases such as NCBI. The included strains grouped into seven clusters based on average nucleotide identity. Strains designated as B. aerophilus, B. altitudinis, and B. stratosphericus grouped together in the cluster containing the B. altitudinis type strain, suggesting that these three species should be considered a single species, B. altitudinis. The antimicrobial activity of UTK D1-0055 was evaluated against a panel of 15 Listeria strains (nine Listeria monocytogenes serotypes, Listeria innocua, and Listeria marthii), other foodborne pathogens (six Salmonella enterica serotypes and Escherichia coli), and three representative fungi (Saccharomyces cerevisiae, Botrytis cinerea, and Hyperdermium pulvinatum). Antibacterial activity was observed against all Listeria strains, but no antibacterial effects were found against the other tested bacterial and fungal strains. Biosynthetic gene clusters were identified in silico that may be related to the observed antibacterial activity, and these clusters included genes that putatively encode bacteriocins and nonribosomally synthesized peptides. The B. altitudinis strain identified in this investigation had a broad range of antilisterial activity, suggesting that it and other related strains may be useful for biocontrol in the food industry. HIGHLIGHTS