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Academic literature on the topic 'Hydrogen Peroxide Biosensor'

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Published: 14 March 2023

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Journal articles on the topic "Hydrogen Peroxide Biosensor"

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Baccar, H., T. Ktari, and A. Abdelghani. "Functionalized Palladium Nanoparticles for Hydrogen Peroxide Biosensor." International Journal of Electrochemistry 2011 (2011): 1–4. http://dx.doi.org/10.4061/2011/603257.

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We present a comparison between two biosensors for hydrogen peroxide (H2O2) detection. The first biosensor was developed by the immobilization of Horseradish Peroxidase (HRP) enzyme on thiol-modified gold electrode. The second biosensor was developed by the immobilization of cysteamine functionalizing palladium nanoparticles on modified gold surface. The amino groups can be activated with glutaraldehyde for horseradish peroxidase immobilization. The detection of hydrogen peroxide was successfully observed in PBS for both biosensors using the cyclic voltammetry and the chronoamperometry techniques. The results show that the limit detection depends on the large surface-to-volume ratio attained with palladium nanoparticles. The second biosensor presents a better detection limit of 7.5 μM in comparison with the first one which is equal to 75 μM.
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Valencia, Germán Ayala, Luci Cristina de Oliveira Vercik, and Andrés Vercik. "A new conductometric biosensor based on horseradish peroxidase immobilized on chitosan and chitosan/gold nanoparticle films." Journal of Polymer Engineering 34, no. 7 (September 1, 2014): 633–38. http://dx.doi.org/10.1515/polyeng-2014-0072.

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Abstract A new conductometric biosensor was developed and characterized; the biosensor was based on horseradish peroxidase that was deposited in chitosan and chitosan/AuNPs films. The biosensors were characterized by scanning electron microscopy and current-voltage curves. Current-voltage curves in biosensors showed that the electrical conductivity and bistability in biosensors can be modulated by horseradish peroxidase. Horseradish peroxidase catalyzed the reduction of H2 O2 to H2 O with the oxidation of the prosthetic group (Fe3+) in the enzyme to Fe4+=O. Conductometric signal in the biosensors increased with the gradual increase of H2 O2 concentration, and it was due to the H2 O2 reduction. Linear hydrogen peroxide detection was observed for a concentration between 0 and 15 mm. The results proved that these biosensors could have promising industrial applications, due to its rapid and sensitive H2 O2 detection.
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Baccar, Zouhair M., and Imène Hafaiedh. "Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor Application." International Journal of Electrochemistry 2011 (2011): 1–5. http://dx.doi.org/10.4061/2011/934893.

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We present a new biosensor for hydrogen peroxide (H2O2) detection. The biosensor was based on the immobilization of horseradish peroxidase (HRP) enzyme on layered double hydroxides- (LDH-) modified gold surface. The hydrotalcite LDH (Mg2Al) was prepared by coprecipitation in constant pH and in ambient temperature. The immobilization of the peroxidase on layered hybrid materials was realized via electrostatic adsorption autoassembly process. The detection of hydrogen peroxide was successfully observed in PBS buffer with cyclic voltammetry and the chronoamperometry techniques. A limit detection of 9 μM of H2O2was obtained with a good reproducibility. We investigate the sensitivity of our developed biosensor for H2O2detection in raw milk.
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Zhao, Dong Jiao, Yan Hong Chen, Chen Di Tu, Yao Fang Xuan, and Feng Na Xi. "Construction of Reagentless Biosensor Based on Self-Assembly and Electrodeposition for Determination of Hydrogen Peroxide." Advanced Materials Research 441 (January 2012): 442–46. http://dx.doi.org/10.4028/www.scientific.net/amr.441.442.

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A general methodology to prepare reagentless biosensor was developed based on self-assembly and electrodeposition. Redox active inorganic multilayers consisting of copper hexacyanoferrate (CuHCF) multilayers were formed by successive self-assembly. A simple and controllable electrodeposition approach was established for one-step fabrication of chitosan-enzyme layer on CuHCF modified electrode. Horseradish peroxidase was selected as the model enzyme. With CuHCF as the electroactive mediator, the developed reagentless biosensor exhibited a fast amperometric response for the determination of hydrogen peroxide (H2O2). The linear response ranged from 1.4 × 10-5 to 2.0 × 10-4 M with a detection limit of 1.2 × 10-6 M. The biosensor exhibited high reproducibility and long-time storage stability. The proposed methodology could serve as a versatile platform for fabrication of electrochemical biosensors.
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Kafi, A. K. M., Dong-Yun Lee, Sang-Hyun Park, and Young-Soo Kwon. "A Hydrogen Peroxide Biosensor Based on Peroxidase Activity of Hemoglobin in Polymeric Film." Journal of Nanoscience and Nanotechnology 7, no. 11 (November 1, 2007): 4005–8. http://dx.doi.org/10.1166/jnn.2007.095.

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A Hydrogen peroxide (H2O2) biosensor, based on hemoglobin (Hb) and ortho-phenylenediamine (o-PD) gold electrode, was fabricated. Hb was immobilized onto the electrode surface by electrochemical polymerize method with o-PD. The designed biosensor showed a well defined redox peak which was attributed to the direct electrochemical response of Hb. The immobilized Hb exhibited an excellent electrocatalytical response to the reduction of hydrogen peroxide, enabling the sensitivity determination of H2O2. Factors and performances such as pH, potential, influencing the designed biosensor, were studied carefully. The amperometric detection of H2O2 was carried out at −300 mV in phosphate buffer solution (PBS) (0.1 M) with pH 6.0. This biosensor showed a fast amperometric response (less then 5 s) to H2O2. The levels of the (Relative standard deviation) RSDs (<3 5%) for the entire analyses reflected a highly reproducible sensor performance. Using the optimized conditions, the detection limit of the biosensor was 1 × 10−7 M and linear range was from 5 × 10−6 to 1.25 × 10−4 M. In addition, this sensor showed long term stability and good sensitivity.
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Nguyen Duc, Nghia, Chinh Huynh Dang, Hoang Tran Vinh, and Vu Dao Hong. "Peroxidase-like activity of Fe3O4/carbon core-shell nanostructured : effects of carbon shell thickness for application to glucose biosensor." Vietnam Journal of Catalysis and Adsorption 10, no. 2 (July 30, 2021): 109–13. http://dx.doi.org/10.51316/jca.2021.038.

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In this study, we present a protocol for synthesis of carbon coated Fe3O4 nanoparticles with core-shell structured nanocomposite (FeC) following a two steps approach. The peroxidase-like acitivity of the synthesized FeC nanocomposite has been evaluated towards replacing of the horseradish peroxidase enzyme (HRP) in hydrogen peroxide enzymatic biosensor. In which, FeC has catalyzed for a redox reaction 5,5'-tetramethylbenzidine (TMB) and H2O2 to produce oxidized state of TMB with as a blue color. Results exhibited that FeC has a high catalytic activity accepting for fabrication of a high selectivity hydrogen peroxide (H2O2) colorimetric sensor with low detection of limit (LoD) of 0.02 mM H2O2. Based on this finding, we have used FeC and combined with glucose oxidase (GOx) enzyme to construct a new colorimetric glucose biosensor with high selectivity.
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Chmayssem, Ayman, Ibrahim Shalayel, Stéphane Marinesco, and Abdelkader Zebda. "Investigation of GOx Stability in a Chitosan Matrix: Applications for Enzymatic Electrodes." Sensors 23, no. 1 (January 1, 2023): 465. http://dx.doi.org/10.3390/s23010465.

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In this study, we designed a new biosensing membrane for the development of an electrochemical glucose biosensor. To proceed, we used a chitosan-based hydrogel that entraps glucose oxidase enzyme (GOx), and we crosslinked the whole matrix using glutaraldehyde, which is known for its quick and reactive crosslinking behavior. Then, the stability of the designed biosensors was investigated over time, according to different storage conditions (in PBS solution at temperatures of 4 °C and 37 °C and in the presence or absence of glucose). In some specific conditions, we found that our biosensor is capable of maintaining its stability for more than six months of storage. We also included catalase to protect the biosensing membranes from the enzymatic reaction by-products (e.g., hydrogen peroxide). This design protects the biocatalytic activity of GOx and enhances the lifetime of the biosensor.
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Zhang, Yunfei, Tingting Lin, Yi Shen, and Hongying Li. "A High-Performance Self-Supporting Electrochemical Biosensor to Detect Aflatoxin B1." Biosensors 12, no. 10 (October 20, 2022): 897. http://dx.doi.org/10.3390/bios12100897.

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High-performance electrochemical biosensors for the rapid detection of aflatoxin B1 (AFB1) are urgently required in the food industry. Herein, a multi-scaled electrochemical biosensor was fabricated by assembling carboxylated polystyrene nanospheres, an aptamer and horseradish peroxidase into a free-standing carbon nanofiber/carbon felt support. The resulting electrochemical biosensor possessed an exceptional performance, owing to the unique structures as well as the synergistic effects of the components. The 3D porous carbon nanofiber/carbon felt support served as an ideal substrate, owing to the excellent conductivity and facile diffusion of the reactants. The integration of carboxylated polystyrene nanospheres with horseradish peroxidase was employed as a signal amplification probe to enhance the electrochemical responses via catalyzing the decomposition of hydrogen peroxide. With the aid of the aptamer, the prepared sensors could quantitatively detect AFB1 in wine and soy sauce samples via differential pulse voltammetry. The recovery rates of AFB1 in the samples were between 87.53% and 106.71%. The limit of detection of the biosensors was 0.016 pg mL−1. The electrochemical biosensors also had excellent sensitivity, reproducibility, specificity and stability. The synthetic strategy reported in this work could pave a new route to fabricate high-performance electrochemical biosensors for the detection of mycotoxins.
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Gurbanova, Lala. "Sensors for analysis of hydrogen peroxide." Scientific Bulletin 3 (2020): 169–74. http://dx.doi.org/10.54414/bgif9220.

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The physico-chemical properties of new type catalase sensors, the so-called biomimetic sensors modulating some of catalase biosensor functions were investigated. These sensors have technological advantages ever their biological analogs due to the properties usually attributed to chemical sensors. The development electrochemical system stands in between bio- and chemical sensors.
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Karunakaran, Chandran, Murugesan Karthikeyan, Marimuthu Dhinesh Kumar, Ganesan Kaniraja, and Kalpana Bhargava. "Electrochemical Biosensors for Point of care Applications." Defence Science Journal 70, no. 5 (October 8, 2020): 549–56. http://dx.doi.org/10.14429/dsj.70.16359.

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Biosensor refers to powerful and innovative analytical tool involving biological sensing element and transducer with broad range of applications, such as diagnosis, drug discovery, biomedicine, food safety and processing, environmental monitoring, security and defense. Recent advances in the field of biotechnology, microelectronics, and nanotechnology have improved the development of biosensors. Glucometers utilizing the electrochemical determination of oxygen or hydrogen peroxide employing immobilised glucose oxidase electrode seeded the discovery and development of biosensors. Molecular recognition based on geometry and forces of interaction play an important role in the biosensor development. The advent of nanotechnology led to highly efficient and sensitive biosensors. They also provide an effective immobilisation matrix for the various bioreceptors. Enzymatic and their mimetic (metalloporphyrin)-based biosensors for reactive oxygen, nitrogen species and cytochrome c will also be discussed. The role of antibodies and their applications in immunosensors development for cytochrome c and superoxide dismutase will be highlighted. The electrochemical biosensors are less expensive, miniaturised and used for point-of-care applications. Further, the fabrication of labVIEW based virtual biosensor instrumentation and microcontroller based portable biosensor for wide variety of applications also devices will be presented.
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Dissertations / Theses on the topic "Hydrogen Peroxide Biosensor"

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Ndangili, Peter Munyao. "Amperometric biosensor systems prepared on poly (aniline-ferrocenium hexafluorophosphate) composites doped with poly(vinyl sulfonic acid sodium salt)." Thesis, University of the Western Cape, 2008. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_6605_1263418223.

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The main hypothesis in this study is the development of a nanocomposite mediated amperometric biosensor for detection of hydrogen peroxide. The aim is to combine the electrochemical properties of both polyaniline and ferrocenium hexafluorophosphate into highly conductive nano composites capable of exhibiting electrochemistry in non acidic media
shuttling electrons between HRP and GCE for biosensor applications.

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Arthur, Joshua N. "Hygroscopic insulator organic field effect transistor sensors." Thesis, Queensland University of Technology, 2022. https://eprints.qut.edu.au/232689/1/Joshua_Arthur_Thesis.pdf.

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Hygroscopic insulator field effect transistors (HIFETs) are organic transistors with promising characteristics for biosensing applications. However, their fundamental sensing mechanisms are not yet fully understood. This thesis explores HIFET sensors through detailed electrical and optical characterisation, providing vital insights into the distinct mechanisms by which HIFETs detect biologically relevant chemicals. Hydrogen peroxide, a by-product of enzymatic reactions, oxidises the organic semiconductor, modulating the output current. Ionic solutions, such as KCl, NaCl and HCl, modulate the current by changing double layer capacitance. These insights are foundational for the continued development of HIFETs as effective multipurpose biosensing platforms.
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Kapita, Patrick Mvemba. "Development of Measurement Systems for Biosensing Applications." Doctoral thesis, Università di Siena, 2020. http://hdl.handle.net/11365/1111250.

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A health condition called “Oxidative Stress” (OS), resulting from an excessive level of Reactive Oxygen Species (ROS) is a “state harmful to the body, which arises when oxidative reactions exceed antioxidant reactions because the balance between them has been lost”[1] OS appears to be associated with and might be a cause of, many serious diseases such as cardio-vascular accidents, cancer, Parkinson’s and Alzheimer’s[2]. This is not surprising as ROS are free oxygen radicals that can attack lipids, proteins, cellular membranes, enzymes and even modify DNA. Extensive correlation studies have shown that the complex impedance spectrum of blood samples from patients diagnosed with an OS syndrome differs significantly from the spectra obtained from the blood of healthy people, which is quite normal as the presence of an excessive amount of ROS should affect the physico-chemical properties of a blood sample. Measuring the complex impedance spectrum of a blood sample can be done quickly by means of low-cost electronic devices, making possible and affordable the early detection of OS among a large population. In order to quantitatively evaluate the OS, the impedance spectra being insufficient, the concentration of oxidative stress markers such as hydrogen peroxyde, malondialdehyde or F2 isoprostanes needs to be measured. Such measurements can, for instance, be used for monitoring the severity of a disease during a treatment. These concentration measurements are traditionally based upon analytical techniques but recently biosensors acting as transducers transforming directly a specific biochemical reaction into a measurable signal have been developed. They are essentially obtained by modifying the surface of metal or carbon electrodes using biomaterials such as enzymes antibodies or DNA that allow bindings or catalytic reactions with other specific biomaterials to occur on the surface of the electrodes. The resulting modifications of the electrical properties of the medium separating the electrodes can be analyzed through ad-hoc electronic and signal processing systems to yield the desired concentration. Biosensors have the advantages of rapid analysis, low-ost and high-precision. They are widely used in various fields, such as medical care, disease diagnosis and food analysis [3]. Hydrogen peroxide (H2O2) generated by cellular processes directly via two-electron reduction of molecular oxygen or indirectly via dismutation of superoxide, is the most widely studied ROS and its overproduction results in OS. Therefore, an ability to quantify the level of hydrogen peroxide and by ricochet the assessment of oxidative stress can be useful in order to assess certain health conditions occurring inside the body and as a result, an integrated electrochemical biosensor coupled with the hydrogen peroxide quantification can become a practical solution as a point of care device at home[4] Most of the time, H2O2 biosensors are based on HRP (Horseradish peroxidase) which is the most commonly used enzyme in the design of biosensors that can supervise the activity of oxidases and determine in terms of concentration, oxidase substrate such as lactate oxidase, cholesterol oxidase, or glucose oxidase, which all induce the production of hydrogen peroxide (HRP’s substrate). In the first part of this research, we explore the development of low-cost and compact measurement systems aiming to determining the impedance of biological samples as they grant access to information from electrical cellular characteristics. It is indeed possible to measure capacitance or conductance that are dependent on the health state of cells. The development of such measurement systems allowing the portability of biological essays requires sensitive electronics. Afterward, in the second part of our work, we explore the design of an electrochemical biosensor by immobilizing an enzyme (HRP) onto the surface of golden electrodes in order to detect and assess the analyte, hydrogen peroxide (H2O2). We also discuss the design of a potentiostat readout circuit to measure and convert the biosensor’s current. The combined results of the two parts of this work can be considered as a first prototype of a low cost and robust instrument easy to use in the field, away from a biological laboratory, with the goal of reaching the so called “point of care diagnostic” [5] The present thesis is organized as follows: Chapter I, introduces the present thesis. In Chapter II, we provide an overview in the field of biosensing technology. Chapter III deals with the design of a portable EIS measurement system to investigate reactive oxygen species in blood. Chapter IV presents an improved version of the previously designed instrument. Moreover, it points out the significance of EIS-based blood analysis through relevant medical diagnosis parameters such as hematocrit and erythrocyte sedimentation rate, extracted from the measured impedance spectra. In Chapter V we discuss on one hand the design of the H2O2 biosensor, and on the other hand the realization of the front-end circuit of the amperometric sensor. Finally, in Chapter VI, a conclusion is drawn..
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Araminaitė, Rūta. "Study of electrocatalytic processes at Prussian blue modified glassy carbon electrode." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2010. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20100213_101926-62386.

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The main purpose of this work is study of electrochemical hydrogen peroxide and ascorbate reactions on electrodes modified by Prussian blue (PB), with the aim to apply these electrodes in creation of sensors and biosensors. For this purpose, a detailed study of electrochemical reduction of hydrogen peroxide, as well as of oxidation of ascorbate at Prussian blue modified rotating disk electrode. In view of the results obtained, a mechanism for hydrogen peroxide reduction at PB modified electrode has been proposed. In accordance with this mechanism, electron transfer appears to be rate-limiting step. The kinetics of decomposition of PB modified electrode in the course of a cathodic reduction of hydrogen peroxide has been studied, and the influence of different factors to this process has been determined. Prototypes of sensors and biosensors, for different analytes have been elaborated and tested.
Darbo tikslas yra elektrocheminių vandenilio peroksido ir askorbato reakcijų tyrimas ant Berlyno mėlynuoju (BM) modifikuotų elektrodų, siekiant pritaikyti šiuos elektrodus jutiklių ir biojutiklių kūrimui. Ištirta vandenilio peroksido redukciją ir askorbato oksidaciją naudojant sukamojo disko elektrodą. Gauti rezultatai galimai įrodo stadijinį vandenilio peroksido katodinės redukcijos mechanizmą vykstantį ant BM modifikuoto elektrodo. Detaliai ištirta BM sluoksnio irimo kinetika vandenilio peroksido elektroredukcijos metu, ir nustatyti faktoriai, įtakojantys irimo proceso greitį. Sukurti jutiklių ir biojutiklių prototipai, kurie galėtų būti panaudoti biologiškai aktyvių medžiagų (vandenilio peroksido, askorbato, gliukozės) nustatymui.
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Araminaitė, Rūta. "Elektrokatalizinių procesų tyrimas ant Berlyno mėlynuoju modifikuoto stiklo anglies elektrodo." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2010. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20100213_101935-42979.

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Darbo tikslas yra elektrocheminių vandenilio peroksido ir askorbato reakcijų tyrimas ant Berlyno mėlynuoju (BM) modifikuotų elektrodų, siekiant pritaikyti šiuos elektrodus jutiklių ir biojutiklių kūrimui. Ištirta vandenilio peroksido redukciją ir askorbato oksidaciją naudojant sukamojo disko elektrodą. Gauti rezultatai galimai įrodo stadijinį vandenilio peroksido katodinės redukcijos mechanizmą vykstantį ant BM modifikuoto elektrodo. Detaliai ištirta BM sluoksnio irimo kinetika vandenilio peroksido elektroredukcijos metu, ir nustatyti faktoriai, įtakojantys irimo proceso greitį. Sukurti jutiklių ir biojutiklių prototipai, kurie galėtų būti panaudoti biologiškai aktyvių medžiagų (vandenilio peroksido, askorbato, gliukozės) nustatymui.
The main purpose of this work is study of electrochemical hydrogen peroxide and ascorbate reactions on electrodes modified by Prussian blue (PB), with the aim to apply these electrodes in creation of sensors and biosensors. For this purpose, a detailed study of electrochemical reduction of hydrogen peroxide, as well as of oxidation of ascorbate at Prussian blue modified rotating disk electrode. In view of the results obtained, a mechanism for hydrogen peroxide reduction at PB modified electrode has been proposed. In accordance with this mechanism, electron transfer appears to be rate-limiting step. The kinetics of decomposition of PB modified electrode in the course of a cathodic reduction of hydrogen peroxide has been studied, and the influence of different factors to this process has been determined. Prototypes of sensors and biosensors, for different analytes have been elaborated and tested.
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Mendes, Renata Kelly. "Investigação dos efeitos dos procedimentos de imobilização em monocamadas auto-organizadas da enzima peroidase no desenvolvimento de um biossensor." [s.n.], 2006. http://repositorio.unicamp.br/jspui/handle/REPOSIP/248400.

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Orientador: Lauro Tatsuo Kubota
Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Quimica
Made available in DSpace on 2018-08-10T12:02:32Z (GMT). No. of bitstreams: 1 Mendes_RenataKelly_D.pdf: 1152307 bytes, checksum: c6e7b97417285a7bb4d70d28ba880353 (MD5) Previous issue date: 2006
Resumo: Neste trabalho foram investigados diferentes métodos de imobilização da enzima HRP empregando como matrizes as monocamadas auto-organizadas formadas sobre eletrodos de ouro, bem como a avaliação da influência do processo de imobilização do elemento biológico no desempenho analítico do biossensor. Para isso. as monocamadas utilizadas foram formadas por meio de tióis com diferentes estruturas, tamanho de suas cadeias carbônicas e grupos terminais. Foi possível constatar que o tamanho da cadeia carbônica de um tioI influencia especialmente no empacotamento da monocamada e, conseqüentemente, na eficácia da imobilização das biomoléculas. Pelos estudos realizados visando a caracterização das SAM sobre a superfície eletródica foi possível verificar que os tióis que possuem em sua cadeia um número menor de carbonos (< 9) tendem a formar monocamadas com uma quantidade considerável de defeitos na superfície do ouro, o que leva a um recobrimento mais baixo. No entanto, os tióis que contém um número mais elevado de carbonos na cadeia apresentam um grau de recobrimento mais elevado e, no entanto, não são boas matrizes para biossensores eletroquímicos, pois podem passivar a superfície, diminuindo a transferência de elétrons e, como conseqüência, a sensibilidade do eletrodo. Quanto a imobilização da enzima nos eletrodos de ouro, verificou-se, por diferentes técnicas, que as monocamadas que possuem grupo terminal -NH2 foram aquelas que proporcionaram os melhores resultados, provavelmente devido ao uso do glutaraldeído como ligante no processo de imobilização. Ao analisar adicionalmente o desempenho do biossensor para a determinação de peróxido de hidrogênio, verificou-se que a SAM formada pela cisteamina é a mais adequada para a imobilização da HRP, por propiciar tanto uma melhor eficácia na adsorção enzima quanto uma sensibilidade mais elevada para H2O2
Abstract: In this work different immobilization procedures of HRP were investigated using as support mIatrices the self-assembled monolayers formed on gold electrodes, as well as the evaluation of the influence of these immobilization processes in the biosensor performance. For this, the used monolayers were prepared by thiols with different structures, carbon chains size and terminal groups. It was possible to have evidence that the thiol carbon chain size influences especially in the coverage monolayer and, consequently, in the efficiency of the biomolecule immobilization. From the studies carried out for the SAM characterization on the electrode surface it was possible to verify that thiols with smaller chain (n<9) trends to form monolayers with a considerable amount of defects on gold surface, that it leads to a lower coverage. However, the thiols with a higher carbon chain present a higher coverage degree, are not being good matrices for electrochemical biosensors, because it can passive the surface, making difficult the electron transfer and, consequently, the electrode sensitivity. In relation to the enzyme immobilization on gold electrodes it was verified, for different techniques, that monolayers that possess -NH2 terminal group provided the best results, probably due to the use of glutharaldeyde as ligant at the immobilization process. Analyzing the biosensor performance for the hydrogen peroxide determination was verified that SAM formed by cysteamine is more adequate for HRP immobilization, because provide the better efficiency in the enzyme immobilization associated to high sensitivity for H2O2
Doutorado
Quimica Analitica
Doutor em Ciências
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Gonçales, Vinícius Romero. "Nanoestruturação de filmes finos para utilização em eletrodos enzimáticos." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/46/46136/tde-31012012-141924/.

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Os desafios atuais no desenvolvimento de biossensores abrangem diversos aspectos, tais como a necessidade de se aperfeiçoar a interface de contato entre o substrato e o material biológico, a eficiência de transdução do sinal químico em um sinal mensurável, o tempo de resposta, a compatibilidade dos biossensores com matrizes biológicas e a integração de diferentes elementos de reconhecimento biológico em um único dispositivo, visando a detecção de distintos analitos. Nesse contexto, o desenvolvimento da nanociência tem criado recursos bastante atraentes para otimizar os aspectos descritos acima. O presente trabalho apresenta, portanto, estudos realizados para a construção de mediadores nanoestruturados que possam operar de maneira mais eficiente que os correspondentes materiais maciços (sistemas não-nanoestruturados). Em uma das abordagens utilizadas, um mediador híbrido de hexacianoferrato de cobre/polipirrol (CuHCNFe/Ppy) teve suas propriedades eletroquímicas aliadas às propriedades morfológicas e eletrônicas de um feltro revestido com nanotubos de carbono do tipo \"cup-stacked\" (feltro/NTCCS) para o desenvolvimento de um sensor de H2O2. O feltro/NTCCS é uma malha hidrofílica condutora que permite uma dispersão bastante uniforme do mediador híbrido. Essa característica, aliada ao aumento da área eletroativa e à interação eletrônica existente entre o CuHCNFe/PPy e os nanotubos de carbono criaram uma plataforma favorável para a construção de um biossensor de glicose. Em uma segunda estratégia, esferas de poliestireno com diâmetros de 300, 460, 600 e 800 nm foram utilizadas como molde para a formação de filmes de CuHCNFe/PPy macroporosos. Os distintos mediadores foram aplicados na detecção de H2O2 com o intuito de se correlacionar a importância do tamanho do poro com o desempenho analítico obtido. Diferentemente do esperado, os mediadores maciços e porosos apresentaram desempenhos analíticos bastante similares, o que levou a uma consideração das propriedades termodinâmicas de superfícies curvas, da molhabilidade de materiais porosos e da influência da cinética eletroquímica na utilização de sistemas porosos. Tais plataformas também foram aplicadas com sucesso na construção de biossensores de glicose e de colina. Por fim, foi possível sintetizar mediadores nanoestruturados através da imobilização de camadas de azul da Prússia e de CuHCNFe dentro das cavidades de filmes de TiO2 mesoporosos (13, 20 e 40 nm de diâmetro). Os resultados obtidos demonstraram a possibilidade de se modular o desempenho dos sensores de H2O2 em função do diâmetro dos poros e da quantidade de mediador imobilizado. A união dos resultados analíticos obtidos com os dados de microscopia eletrônica de varredura possibilitou observar a importância do efeito de confinamento no desempenho dos mediadores. Além disso, dados espectroscópicos na região do visível foram fundamentais para relacionar a presença de defeitos estruturais com a reatividade do material. No fim, tais plataformas foram utilizadas para a formulação de biossensores de colina.
Nowadays, the challenges in the development of biosensors cover various aspects such as the need to improve the interface between the substrate and the biological material, the efficiency of the chemical signal transduction in a measurable one, the response time, the compatibility with biological matrices and the integration of different biological recognition elements in a single device, in order to perform detections of different analytes. In this context, the development of nanoscience has created very attractive features to optimize the aspects described above. Consequently, the present work studies the build up of nanostructured transducers that can operate more efficiently than the corresponding bulk materials (systems non-nanostructured). In one of the approaches used, a hybrid transducer consisting of copper hexacyanoferrate/polypyrrole (CuHCNFe/Ppy) had its electrochemical properties combined with the morphological and electronic properties of a felt decorated with cup-stacked type carbon nanotubes (felt/CSCNT) for development of a H2O2 sensor. Felt/CSCNT is a hydrophilic conductive mesh that allows a uniform dispersion of the hybrid transducer. This feature, coupled with the improvement of electroactive surface and with the electronic interaction among the CuHCNFe/Ppy and carbon nanotubes have created a favorable platform for the construction of a glucose biosensor. In a second strategy, polystyrene spheres with diameters of 300, 460, 600 and 800 nm were used as templates for the formation of macroporous CuHCNFe/Ppy films. The transducers were used to detect H2O2 in order to correlate the importance of pore size with the obtained analytical performance. Unlike expected, porous and bulk transducers presented very similar analytical performances, which led to a consideration of the thermodynamic properties of curved surfaces, the wettability of porous materials and the influence of electrochemical kinetics during the use of porous systems. Such platforms have also been successfully applied in the preparation of glucose and choline biosensors. Finally, it was possible to synthesize nanostructured transducers through the immobilization of Prussian blue layers and CuHCNFe inside the cavities of mesoporous TiO2 films (pore diameters of 13, 20 and 40 nm). The obtained results demonstrated the possibility of modulating the performance of H2O2 sensors according to the pore diameter and the amount of immobilized transducer. The union of the obtained analytical results with scanning electron microscopy data showed the importance of confinement effect on the transducers performances. In addition, spectroscopic data in the visible region were essential to correlate the presence of structural defects with the material reactivity. In the end, these platforms were used for the formulation of choline biosensors.
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Valencia, Germán Ayala. "Transporte eletrônico em biofilmes nanoestruturados para biossensores a base de enzimas." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/74/74132/tde-10072013-095546/.

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Os biossensores são dispositivos empregados para a detecção de um analito específico, podendo assim ser no controle de qualidade nos alimentos para determinar a presença de micro-organismos, toxinas ou metabólitos. O presente estudo objetiva desenvolver um biossensor condutométrico, baseado na imobilização de peroxidasse em membranas de quitosana e quitosana com nanopartículas de ouro (AuNP) para a detecção de peroxido de hidrogênio. O trabalho foi dividido em três etapas. Na primeira etapa foi estudada a obtenção de AuNP empregando agentes redutores biológicos, sendo avaliados três monossacarídeos (glicose, frutose e galactose), três dissacarídeos (sacarose, maltose e lactose), dois biopolímeros (amido e quitosana), assim como os extratos obtidos a partir das folhas de hortelã (Mentha piperita) e cascas de furtas de abacaxi (Ananas comosus), banana (Musa sp. ), maracujá (Passiflora edulis), tangerina (Citrus reticulata). A quitosana mostrou-se como o melhor agente redutor na síntese das AuNP, as quais foram empregadas na segunda etapa para a produção de membranas. Três tipos de membranas foram processadas, membranas de quitosana sem AuNP e membranas de quitosana com AuNP com concentrações de 8 e 11mM., as quais foram caraterizadas morfológica e eletricamente. Finalmente foi avaliada a imobilização da peroxidasse usando quatro tratamentos diferentes, sendo a dispersão da peroxidasse nas soluções filmogênicas precursoras das membranas a mais eficiente. A resposta elétrica destas membranas é dependente da concentração de AuNP e da presença de enzimas, e também foi alterada quando as mesmas foram expostas a soluções de tampão fosfato com diferentes concentrações de peroxido de hidrogênio. Isto constitui o principio de operação dos biossensores condutométricos desenvolvidos neste trabalho.
Biosensors are devices used for detecting a specific analyte, and thus can be used in quality control of food for determining the presence of micro-organisms, toxins or metabolites. The present study aims to develop a conductometric biosensor based on the immobilization of peroxidase in membranes of chitosan and chitosan with gold nanoparticles (AuNP) for the detection of hydrogen peroxide. The work was divided into three stages. In the first stage, methods for obtaining AuNP employing biological reducing agents were studied, evaluating three monosaccharides (glucose, fructose and galactose), three disaccharides (sucrose, maltose and lactose), two biopolymers (starch and chitosan), as well as the extracts obtained from the leaves of mint (Mentha piperita) and husks dost thou pineapple (Ananas comosus), banana (Musa sp), passion fruit (Passiflora edulis), mandarin (Citrus reticulata). Chitosan exhibited the best behavior as reducing agent for the synthesis of AuNP, which were employed in the second step for the production of membranes. Three types of membranes were processed, chitosan membranes without AuNP and chitosan membranes with AuNP with concentrations of 8 and 11mM, which were morphologically and electrically characterized. Finally the peroxidase immobilization was evaluated using four different procedures, being the dispersion of the peroxidase in filmogenic solutions precursor of membranes the more efficient. The electrical response of these membranes, depends on the AuNP concentration and the presence of enzymes, and was also altered when they were exposed to hydrogen peroxide containing phosphate buffer solutions. This constitutes the principle of operation of the conductometric biosensor developed in this work.
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Jhai, You-syuan, and 翟祐暄. "Fabrication of Amperometric Hydrogen Peroxide Biosensor Based on Trisoctahedral Nano-Metallic Catalyst and its Applications." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/7a9fgh.

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碩士
國立臺灣科技大學
化學工程系
100
This study was comprised of two parts: (I) the synthesis of trisoctahedral gold nanocrystal (AuNC) and core-shell AuNCs@Pt catalysts for the applications of electrochemical sensing of hydrogen peroxide; (II) the immobilization of enzymatic species by adding cross-linking agent to fabricate electrochemical glucose sensor. For the first part, the surface morphology and crystal lattice of the synthesized nano-metallic catalysts were investigated using SEM, XRD. The synthesized AuNCs showed many active sites due to their polyhedral structure. In order to enhance the catalytic ability, the AuNCs with outer layer of platinum (to form polyhedral nano platinum crystals) was synthesized which provides a particular advantage of only a small amout of platinum was needed. The results of UV-vis spectropy and electrochemical acid treatment showed that the bimetallic catalyst is core-shell structure which was almost completely covered by reduced platinum. The detection of hydrogen peroxide was measured by electrochemical methods. Moreoer, the optimized parameters for electrochemical analyses including the applied voltage and the surface protection layer were applied for the detection of hydrogen peroxide. The results showed that the detection limit of 10 μM, with a linear range of detection from 0.01 to 5.1 mM (R2=0.997), high sensitivity of 397.37 μA/(mMcm2), and excellent anti-interfering ability were obtained for the prepared sensing system. For the second part, the prepared sensing layer on the electrodes was further applied for the detection of glucose. In this study, the synthesized AuNCs were used to adsorb enzyme molecules which were followed by the addition of cross-linking agent to ensure the combination. The enzyme loading and operating parameters were optimized. It showed that the assembled sensor prepared under the optimized condition provided mild environment for enzyme immobilization and facilitated the bio-reaction between enzyme and bio-species, which allowed enzyme exhibiting good affinity and stability. The obtained glucose sensing at 0.5 V v.s Ag/AgCl applied potential showed linear range of 1.0 to 7.0 mM (R2=0.996), with sensitivity of 86.93 μA/mMcm2. We have shown that a highly sensitive glucose biosensor with good reproducibility and precision, high sensitivity, and great stability was successfully prepared.
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Kosto, Yuliia. "Studium tenkých vrstev oxidu ceru pro biosenzorické aplikace." Doctoral thesis, 2021. http://www.nusl.cz/ntk/nusl-439696.

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Title: Study of cerium oxide thin films for biosensing applications Author: Yuliia Kosto Department: Department of Surface and Plasma Science Supervisor: Prof. RNDr. Vladimír Matolín, DrSc. Abstract: The presented scientific work was conducted in two main directions. The first one is an investigation of the simple biomolecules (glycine and sarcosine) bonding to cerium oxide model films by surface science techniques: photoelectron and near-edge X-ray absorption spectroscopies. Adsorption chemistry and thermal stability of the molecules on the oxides were studied in relation to the oxidation state of ceria cations, film morphology, and molecular deposition method. The oxygen vacancies in the oxide were shown to affect the adsorption geometry of glycine and stimulate molecular decomposition. The polycrystalline oxide morphology provided stabilizing effect on the glycine adlayer. Sarcosine deposited in vacuum formed densely packed adlayer with the molecules directed outwards. Interestingly, the results revealed that molecular film deposited from the aqueous solution, in contrast to deposition in vacuum, induces continuous reduction of the cerium oxide during thermal annealing. The second part is a study of polycrystalline cerium oxide thin films as an electrode for electrochemical and electrochemiluminescent...
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Book chapters on the topic "Hydrogen Peroxide Biosensor"

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Luong, John H. T., A. L. Nguyen, and George G. Guilbault. "The principle and technology of hydrogen peroxide based biosensors." In Measurement and Control, 85–115. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/bfb0007388.

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Kirchner, Patrick, Steffen Reisert, and Michael J. Schöning. "Calorimetric Gas Sensors for Hydrogen Peroxide Monitoring in Aseptic Food Processes." In Springer Series on Chemical Sensors and Biosensors, 279–309. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/5346_2013_51.

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Creanga, Carmen, Simona Serban, Robin Pittson, and Nabil El. "“No calibration” type sensor in routine amperometric bio-sensing: An example of a disposable hydrogen peroxide biosensor." In Biosensors - Emerging Materials and Applications. InTech, 2011. http://dx.doi.org/10.5772/17769.

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Csöregi, E., L. Gorton, G. Marko-Varga, A. Tüdös, and T. W. Kok. "Detection of Hydrogen Peroxide and Organic Peroxides in Flow Injection Based on Peroxidase Modified Carbon Fiber Microelectrodes." In Biosensors '94, 217–18. Elsevier, 1994. http://dx.doi.org/10.1016/b978-1-85617-242-4.50176-2.

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Narasaiah, D. "An enzyme electrode for hydrogen peroxide based on peroxidase immobilized on glassy carbon electrode." In Biosensors '92 Proceedings, 211. Elsevier, 1992. http://dx.doi.org/10.1016/b978-1-85617-161-8.50044-6.

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Woedtke, Th v., P. Abel, and U. Fischer. "Amperometric glucose oxidase/hydrogen peroxide glucose sensors: the influence of hydrogen peroxide on the function and its potential use in sensor sterilization." In Biosensors '92 Proceedings, 513. Elsevier, 1992. http://dx.doi.org/10.1016/b978-1-85617-161-8.50143-9.

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Nagiev, Tofik M. "Enzymatic Biosensors and Their Biomimetic Analogs: Advanced Analytical Appliances." In Coherent Synchronized Oxidation Reactions by Hydrogen Peroxide, 289–307. Elsevier, 2007. http://dx.doi.org/10.1016/b978-044452851-3/50009-1.

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Ho, W. O., C. J. McNeil, H. J. Hager, G. P. Evans, and W. H. Mullen. "MEDIATORLESS ELECTROENZYMIC REDUCTION OF HYDROGEN PEROXIDE AT PLATINISED CARBON ELECTRODES = APPLICATION TO IMMUNOASSAY." In Biosensors '92 Proceedings, 383. Elsevier, 1992. http://dx.doi.org/10.1016/b978-1-85617-161-8.50100-2.

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Conference papers on the topic "Hydrogen Peroxide Biosensor"

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Halim, Nur Hamidah Abdul, Lee Yook Heng, and Uda Hashim. "Hydrogen peroxide biosensor based on titanium oxide." In THE 2015 UKM FST POSTGRADUATE COLLOQUIUM: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. AIP Publishing LLC, 2015. http://dx.doi.org/10.1063/1.4931305.

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Ohnuki, H., T. Wako, B. Mecheri, H. Wu, D. Tsuya, and H. Endo. "Self-Powered Hydrogen Peroxide Sensor and Its Biosensor Application." In 2018 International Conference on Solid State Devices and Materials. The Japan Society of Applied Physics, 2018. http://dx.doi.org/10.7567/ssdm.2018.ps-7-24.

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Narayanan, J. Shankara, and Gymama Slaughter. "Gold foil-based biosensor for the determination of hydrogen peroxide." In 2018 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE, 2018. http://dx.doi.org/10.1109/embc.2018.8512851.

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Vasconcelos, Helena, Ana Matias, Pedro Jorge, Cristina Saraiva, João Mendes, João Araújo, Bernardo Dias, Paulo Santos, José M. M. M. Almeida, and Luís C. C. Coelho. "Optical Biosensor for the Detection of Hydrogen Peroxide in Milk." In CSAC2021. Basel Switzerland: MDPI, 2021. http://dx.doi.org/10.3390/csac2021-10466.

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Ding, Yu, Ying Wang, and Yu Lei. "Electrospun hemoglobin microbelts based biosensor for sensitive detection of hydrogen peroxide." In 2011 37th Annual Northeast Bioengineering Conference (NEBEC). IEEE, 2011. http://dx.doi.org/10.1109/nebc.2011.5778598.

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Vasconcelos, Helena, Ana Beatriz Matias Teixeira, João Mendes, João Araújo, Bernardo Dias, Pedro A. S. Jorge, Cristina M. Saraiva, Luis C. C. Coelho, and José Manuel M. M. de Almeida. "Optical biosensor for the detection of low concentrations of hydrogen peroxide in milk samples." In Optical Sensing and Detection VII, edited by Francis Berghmans and Ioanna Zergioti. SPIE, 2022. http://dx.doi.org/10.1117/12.2621552.

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LAN, DAN, and BAOXIN LI. "CHEMILUMINESCENCE FLOW-THROUGH BIOSENSOR FOR HYDROGEN PEROXIDE BASED ON ENHANCED HRP ACTIVITY BY GOLD NANOPARTICLES." In Proceedings of the 15th International Symposium. WORLD SCIENTIFIC, 2008. http://dx.doi.org/10.1142/9789812839589_0049.

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Sirdeshmukh, Vedashree V., Indrayani S. Kadu, Shreshtha S. Mishra, and Anup A. Kale. "A Novel Non-Enzymatic PEDOT:PSS/GO/MnO2 Based Biosensor For Hydrogen Peroxide Detection in Biological Samples." In 2019 IEEE 13th International Conference on Nano/Molecular Medicine & Engineering (NANOMED). IEEE, 2019. http://dx.doi.org/10.1109/nanomed49242.2019.9130613.

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Hsien-Chin (or initial) Wei, Su-Hua (or initial) Huang, and Yeun-Chung (or initial) Lee. "Development of a Novel BIA Enzyme Calorimetric Biosensor and Detection System for Hydrogen Peroxide Determination at Hazardous Level." In 2008 Providence, Rhode Island, June 29 - July 2, 2008. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2008. http://dx.doi.org/10.13031/2013.24873.

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Schubert, Florian, Herbert H. Rinneberg, and Fang Wang. "Fiber optic biosensors for hydrogen peroxide and L-lactate." In International Symposium on Biomedical Optics Europe '94, edited by Anna M. Verga Scheggi, Francesco Baldini, Pierre R. Coulet, and Otto S. Wolfbeis. SPIE, 1995. http://dx.doi.org/10.1117/12.201240.

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