Relevant bibliographies by topics / HvCslF6

Academic literature on the topic 'HvCslF6'

Author: Grafiati
Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "HvCslF6"

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Lim, Wai Li, Helen M. Collins, Caitlin S. Byrt, Jelle Lahnstein, Neil J. Shirley, Matthew K. Aubert, Matthew R. Tucker, Manuela Peukert, Andrea Matros, and Rachel A. Burton. "Overexpression of HvCslF6 in barley grain alters carbohydrate partitioning plus transfer tissue and endosperm development." Journal of Experimental Botany 71, no. 1 (September 19, 2019): 138–53. http://dx.doi.org/10.1093/jxb/erz407.

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Cseh, A., K. Kruppa, I. Molnár, M. Rakszegi, J. Doležel, and M. Molnár-Láng. "Characterization of a new 4BS.7HL wheat–barley translocation line using GISH, FISH, and SSR markers and its effect on the β-glucan content of wheat." Genome 54, no. 10 (October 2011): 795–804. http://dx.doi.org/10.1139/g11-044.

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A spontaneous interspecific Robertsonian translocation was revealed by genomic in situ hybridization (GISH) in the progenies of a monosomic 7H addition line originating from a new wheat ‘Asakaze komugi’ × barley ‘Manas’ hybrid. Fluorescence in situ hybridization (FISH) with repetitive DNA sequences (Afa family, pSc119.2, and pTa71) allowed identification of all wheat chromosomes, including wheat chromosome arm 4BS involved in the translocation. FISH using barley telomere- and centromere-specific repetitive DNA probes (HvT01 and (AGGGAG)n) confirmed that one of the arms of barley chromosome 7H was involved in the translocation. Simple sequence repeat (SSR) markers specific to the long (L) and short (S) arms of barley chromosome 7H identified the translocated chromosome segment as 7HL. Further analysis of the translocation chromosome clarified the physical position of genetically mapped SSRs within 7H, with a special focus on its centromeric region. The presence of the HvCslF6 gene, responsible for (1,3;1,4)-β-d-glucan production, was revealed in the centromeric region of 7HL. An increased (1,3;1,4)-β-d-glucan level was also detected in the translocation line, demonstrating that the HvCslF6 gene is of potential relevance for the manipulation of wheat (1,3;1,4)-β-d-glucan levels.
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Jamahari, Azreena, Wong Ling-Chie, Fan Xioalai, Liu Qiaoquan, Leong Sui Sien, Fauziah Abu Bakar, Amy Halimah Rajaee, Patricia King Jie Hung, Hairul Azman Roslan, and Wong Sie Chuong. "CHARACTERISATION OF HORDEUM VULGARE CELLULOSE SYNTHASE-LIKE F6 PROMOTER VIA TRANSGENE EXPRESSION IN RICE." Malaysian Journal of Science 40, no. 2 (June 30, 2021): 61–86. http://dx.doi.org/10.22452/mjs.vol40no2.6.

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Beta-glucan in cereal crops is known as a functional food, which can reduce cardiovascular diseases by lowering blood cholesterol levels. However, beta-glucan content is relatively low in rice grains, despite being relatively abundant in barley and oat grains. Taking advantage of rice as the staple food for Asians, increasing beta-glucan content in rice for their consumption may help to reduce cardiovascular-related diseases among them. Previous attempts in increasing beta-glucan content in rice via transgene expression of beta-glucan synthase genes from barley into rice were unsuccessful due to the use of non-tissue specific as well as constitutively expressing promoter. The current transgenic expression study was performed to characterise the promoter of beta-glucan synthase gene in barley using beta-glucuronidase (GUS) reporter gene. Two fragments of HvCslF6 promoter (2771 bp and 1257 bp) were successfully fused with GUS reporter gene and integrated into rice plants, demonstrated that the promoter was functional in the heterologous plant system. The presence of blue GUS staining was observed on the leaf, root, stem, and grain of the transgenic rice regardless of the promoter length used and stayed functional up to the next generation. GUS qualitative analysis confirmed that the shorter promoter length generated a stronger GUS activity in comparison to the longer one. This indicated that the presence of repressor elements in between the -2771 bp and -1257 bp regions. The preliminary results shed light on the strong promoter activity in the rice endosperm tissue. It can become an alternative to the collection of plant promoters that can be used for grain quality improvement and biofortification.
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Gajek, Katarzyna, Agnieszka Janiak, Urszula Korotko, Beata Chmielewska, Marek Marzec, and Iwona Szarejko. "Whole Exome Sequencing-Based Identification of a Novel Gene Involved in Root Hair Development in Barley (Hordeum vulgare L.)." International Journal of Molecular Sciences 22, no. 24 (December 14, 2021): 13411. http://dx.doi.org/10.3390/ijms222413411.

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Root hairs play a crucial role in anchoring plants in soil, interaction with microorganisms and nutrient uptake from the rhizosphere. In contrast to Arabidopsis, there is a limited knowledge of root hair morphogenesis in monocots, including barley (Hordeum vulgare L.). We have isolated barley mutant rhp1.e with an abnormal root hair phenotype after chemical mutagenesis of spring cultivar ‘Sebastian’. The development of root hairs was initiated in the mutant but inhibited at the very early stage of tip growth. The length of root hairs reached only 3% of the length of parent cultivar. Using a whole exome sequencing (WES) approach, we identified G1674A mutation in the HORVU1Hr1G077230 gene, located on chromosome 1HL and encoding a cellulose synthase-like C1 protein (HvCSLC1) that might be involved in the xyloglucan (XyG) synthesis in root hairs. The identified mutation led to the retention of the second intron and premature termination of the HvCSLC1 protein. The mutation co-segregated with the abnormal root hair phenotype in the F2 progeny of rhp1.e mutant and its wild-type parent. Additionally, different substitutions in HORVU1Hr1G077230 were found in four other allelic mutants with the same root hair phenotype. Here, we discuss the putative role of HvCSLC1 protein in root hair tube elongation in barley.
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Burton, Rachel A., Stephen A. Jobling, Andrew J. Harvey, Neil J. Shirley, Diane E. Mather, Antony Bacic, and Geoffrey B. Fincher. "The Genetics and Transcriptional Profiles of the Cellulose Synthase-Like HvCslF Gene Family in Barley." Plant Physiology 146, no. 4 (February 7, 2008): 1821–33. http://dx.doi.org/10.1104/pp.107.114694.

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Emebiri, Livinus C. "EST-SSR markers derived from an elite barley cultivar (Hordeum vulgare L. ‘Morex’): polymorphism and genetic marker potential." Genome 52, no. 8 (August 2009): 665–76. http://dx.doi.org/10.1139/g09-040.

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Microsatellites or simple sequence repeats have become the markers of choice for marker-assisted selection because of their low template DNA requirement, high reproducibility, and high level of polymorphism. This study investigated a new set of barley ( Hordeum vulgare L.) EST-derived SSR markers designed to target gene sequences expressed during grain development, as they are more likely to be important in determining grain quality. The EST sequences (HVSMEh and HVSMEi) were derived from cDNA libraries of the elite six-rowed cultivar Morex, made from spikes harvested at 5 to 45 days after pollination. Approximately half of the 110 SSR markers derived from the ESTs were polymorphic in a panel of 8 diverse barley genotypes, with PIC values between 0.19 and 0.79. Twenty of the new markers were mapped to chromosomal locations using 2 doubled haploid populations. To demonstrate marker potential, quantitative trait locus (QTL) analyses were carried out with phenotypic data on wort β-glucan content and β-glucanase activity, two traits with a long history of genetic studies. Most of the EST-SSR markers mapped to within 10 cM of the cellulose synthase (HvCesA) and cellulose synthase-like (HvCslF) genes, which provides highly informative functional markers for tracking these genes in breeding programs. It was also observed that on any given chromosome, the QTL for β-glucan content and β-glucanase activity were rarely coincident but tended to occur in adjacent intervals along chromosomal regions, which agreed with their independent genetic basis; the adjacent localization may be important for coordination of cell wall degradation during germination and malting.
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Burton, Rachel A., Helen M. Collins, Natalie A. J. Kibble, Jessica A. Smith, Neil J. Shirley, Stephen A. Jobling, Marilyn Henderson, et al. "Over-expression of specific HvCslF cellulose synthase-like genes in transgenic barley increases the levels of cell wall (1,3;1,4)-β-d-glucans and alters their fine structure." Plant Biotechnology Journal 9, no. 2 (January 6, 2011): 117–35. http://dx.doi.org/10.1111/j.1467-7652.2010.00532.x.

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Douchkov, Dimitar, Stefanie Lueck, Goetz Hensel, Jochen Kumlehn, Jeyaraman Rajaraman, Annika Johrde, Monika S. Doblin, et al. "The barley (Hordeum vulgare) cellulose synthase-like D2 gene (HvCslD2) mediates penetration resistance to host-adapted and nonhost isolates of the powdery mildew fungus." New Phytologist 212, no. 2 (June 28, 2016): 421–33. http://dx.doi.org/10.1111/nph.14065.

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Garcia-Gimenez, Guillermo, Joanne Russell, Matthew K. Aubert, Geoffrey B. Fincher, Rachel A. Burton, Robbie Waugh, Matthew R. Tucker, and Kelly Houston. "Barley grain (1,3;1,4)-β-glucan content: effects of transcript and sequence variation in genes encoding the corresponding synthase and endohydrolase enzymes." Scientific Reports 9, no. 1 (November 21, 2019). http://dx.doi.org/10.1038/s41598-019-53798-8.

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AbstractThe composition of plant cell walls is important in determining cereal end uses. Unlike other widely consumed cereal grains barley is comparatively rich in (1,3;1,4)-β-glucan, a source of dietary fibre. Previous work showed Cellulose synthase-like genes synthesise (1,3;1,4)-β-glucan in several tissues. HvCslF6 encodes a grain (1,3;1,4)-β-glucan synthase, whereas the function of HvCslF9 is unknown. Here, the relationship between mRNA levels of HvCslF6, HvCslF9, HvGlbI (1,3;1,4)-β-glucan endohydrolase, and (1,3;1,4)-β-glucan content was studied in developing grains of four barley cultivars. HvCslF6 was differentially expressed during mid (8–15 DPA) and late (38 DPA) grain development stages while HvCslF9 transcript was only clearly detected at 8–10 DPA. A peak of HvGlbI expression was detected at 15 DPA. Differences in transcript abundance across the three genes could partially explain variation in grain (1,3;1,4)-β-glucan content in these genotypes. Remarkably narrow sequence variation was found within the HvCslF6 promoter and coding sequence and does not explain variation in (1,3;1,4)-β-glucan content. Our data emphasise the genotype-dependent accumulation of (1,3;1,4)-β-glucan during barley grain development and a role for the balance between hydrolysis and synthesis in determining (1,3;1,4)-β-glucan content, and suggests that other regulatory sequences or proteins are likely to be involved in this trait in developing grain.
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Garcia-Gimenez, Guillermo, Miriam Schreiber, George Dimitroff, Alan Little, Rohan Singh, Geoffrey B. Fincher, Rachel A. Burton, Robbie Waugh, Matthew R. Tucker, and Kelly Houston. "Identification of candidate MYB transcription factors that influence CslF6 expression in barley grain." Frontiers in Plant Science 13 (September 8, 2022). http://dx.doi.org/10.3389/fpls.2022.883139.

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(1,3;1,4)-β-Glucan is a non-cellulosic polysaccharide required for correct barley grain fill and plant development, with industrial relevance in the brewing and the functional food sector. Barley grains contain higher levels of (1,3;1,4)-β-glucan compared to other small grain cereals and this influences their end use, having undesirable effects on brewing and distilling and beneficial effects linked to human health. HvCslF6 is the main gene contributing to (1,3;1,4)-β-glucan biosynthesis in the grain. Here, the transcriptional regulation of HvCslF6 was investigated using an in-silico analysis of transcription factor binding sites (TFBS) in its putative promoter, and functional characterization in a barley protoplast transient expression system. Based on TFBS predictions, TF classes AP2/ERF, MYB, and basic helix-loop-helix (bHLH) were over-represented within a 1,000 bp proximal HvCslF6 promoter region. Dual luciferase assays based on multiple HvCslF6 deletion constructs revealed the promoter fragment driving HvCslF6 expression. Highest HvCslF6 promoter activity was narrowed down to a 51 bp region located −331 bp to −382 bp upstream of the start codon. We combined this with TFBS predictions to identify two MYB TFs: HvMYB61 and HvMYB46/83 as putative activators of HvCslF6 expression. Gene network analyses assigned HvMYB61 to the same co-expression module as HvCslF6 and other primary cellulose synthases (HvCesA1, HvCesA2, and HvCesA6), whereas HvMYB46/83 was assigned to a different module. Based on RNA-seq expression during grain development, HvMYB61 was cloned and tested in the protoplast system. The transient over-expression of HvMYB61 in barley protoplasts suggested a positive regulatory effect on HvCslF6 expression.
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Dissertations / Theses on the topic "HvCslF6"

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Wong, Sie-Chuong. "Regulation of (1,3;1,4)-beta-glucan synthesis in barley (Hordeum vulgare L.) endosperm and leaf tissues." Thesis, 2015. http://hdl.handle.net/2440/93906.

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This project was carried out to explore the regulatory mechanism for (1,3;1,4)-β-glucan synthesis in barley (Hordeum vulgare L.) in both endosperm and leaf tissues. For the grain (1,3;1,4)-β-glucan content regulation study, transcriptional profiles of candidate genes involved in (1,3;1,4)-β-glucan synthesis and degradation, from the developing barley endosperm, were compared across parental lines that had been previously used for grain (1,3;1,4)-β-glucan QTL studies. Correspondence between differences in transcript levels of selected genes and the QTL detected in parental lines was analysed. In the high (1,3;1,4)-β-glucan parent of a mapping population with a grain (1,3;1,4)-β-glucan QTL near/at the location of the HvCslF6 gene, HvCSLF6 transcript levels increased sharply late in endosperm development. In contrast, HvCslF6 transcript levels did not differ between parents of a mapping population in which no grain (1,3;1,4)-β-glucan QTL had been mapped near/at the HvCslF6 gene. Co-transcription of a β-glucan exo-glucanase gene, HvExoIV gene with HvCslF9 early in endosperm development and with HvCslF6 late in endosperm development indicated that HvEXOIV could be involved in (1,3;1,4)-β-glucan synthesis. It has been reported that leaf (1,3;1,4)-β-glucan is degraded when plants are incubated in the dark for prolonged periods and is re-synthesized upon re-exposure to a normal day/night cycle. Thus, to investigate the regulation of leaf (1,3;1,4)-β-glucan, the transcript levels of (1,3;1,4)-β-glucan synthase genes and related genes were profiled during (1,3;1,4)-β-glucan mobilization upon dark incubation. Some of the genes that responded to prolonged dark incubation showed diurnal transcription patterns, even in continuous darkness. Among the (1,3;1,4)-β-glucan synthase candidate genes, only HvCslH1 was up-regulated upon dark incubation. Its transcripts quickly returned to control levels upon re-exposure to the normal day/night cycle. None of the (1,3;1,4)-β-glucan synthase genes were up-regulated upon re- exposure to normal day/night cycles. Consistent with what was observed for HvExoIV, HvCslF6 and HvCslF9 in developing endosperm, HvExoIV seemed to exhibit co-transcription gene with the HvCslH1.
Thesis (Ph.D.) -- University of Adelaide, School of Agriculture, Food and Wine, 2015
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Ermawar, Riksfardini Annisa. "Metabolic engineering of C₄ grasses for biofuel applications." Thesis, 2016. http://hdl.handle.net/2440/103492.

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The amount of (1,3;1,4)-β-D-glucans or mixed-linkage-β-glucans (MLG) in biofuel crops is of interest because this is a source of fermentable hexose sugar. Recent modification of (1,3;1,4)-β-glucan in barley has opened the possibility of increasing the (1,3;1,4)-β-glucan amount in other plants, including bioethanol crops such as sorghum, a C₄ grass. As S. viridis is a model plant for studying C₄ biofuel crops the characteristics of MLG accumulation in S. viridis is of interest. The initial study determines the levels, structure and distribution of MLG in various vegetative tissues and grain in S. viridis, and investigates the relationship with the transcript levels of Cellulose synthase-like (Csl) genes, i.e. genes involve in MLG synthesis. Modification of MLG amount was tested by generating transformants of S. viridis with over-expressing Csl F6 driven by either the oat globulin promoter (ProASGLO) or the constitutive 35S promoter (Pro35S) from barley (HvCslF6) and sorghum (SbCslF6). In addition, a previous study on the over-expression of the (1,3;1,4)-β-glucan synthase in transgenic barley showed an excessive deposition of the polysaccharide in many cell types causing vascular suffocation and sometimes plant death. To resolve this problem this study also aim to identify a sorghum promoter which may be used to regulate the over-expression of (1,3;1,4)-β-D-glucan synthase genes (such as CslF and CslH) and restrict it to certain specific cell types only. Five putative sorghum mesophyll cell-specific promoters from genes involved in C₄ photosynthesis have been fused with a β-glucuronidase (uidA) cDNA. All modifications were tested in planta using stable Agrobacterium-mediated transformation.
Thesis (Ph.D.) (Research by Publication) -- University of Adelaide, School of Agriculture, Food and Wine, 2016.
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