Dissertations / Theses on the topic 'Human-relevant'
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Stokes, Mark Geoffrey. "Task-relevant neural representations within the human brain." Thesis, University of Cambridge, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.612317.
Full textPolischouk, Anya. "Molecular factors relevant to the radiosensitivity of human tumours /." Stockholm, 2003. http://diss.kib.ki.se/2003/91-7349-508-5.
Full textTsai, Yu-Huan. "Investigating human neurolisteriosis with relevant host and bacterial partners." Paris 7, 2014. http://www.theses.fr/2014PA077232.
Full textListeria monocytogenes (Lm) is a bacterial foodbome pathogen that crosses the intestinal barrier via the interaction of its surface protein InIA with its receptor E-cadherin (Ecad), and disseminate within the host to induce listeriosis. Lm can cross the placental barrier in pregnant women resulting in abortion and fetal infection, and cross the blood-CNS barrier to cause neurolisteriosis via a so far unknown mechanism. InIA-Ecad interaction is species-specific, does not occur in wild-type (wt) mice, but does in humanized mice expressing humanized mouse Ecad. InIA has also been "murinized" (InlAm) to interact with mouse Ecad in wt mice. We have shown that InlAm not only interacts with mouse Ecad, but also uses N-cadherin (Ncad) as a receptor, whereas InIA does not. This unanticipated and artifactual InlAm-Ncad interaction promotes bacterial translocation across villous M cells, accompanying with intestinal inflammation and intestinal barrier damage, ail of which are not seen in humans and humanized mouse models permissive to In1A-Ecad interaction. The widely used reference strains are not representative of clinical isolates, based on MLST, a sequence-based typing method. We have shown in a humanized mouse model of listeriosis developed in the laboratory, that the isolates originating from the most prevalent clones responsible for human neurolisteriosis are more virulent and induce far more efficiently neurolisteriosis than isolates from other clonai complexes and reference strains. By use of the humanized mouse model and relevant human CNS isolates, we are investigating the pathogenesis of orally acquired neurolisteriosis, and deciphering the underlying mechanisms of neurolisteriosis
Abdelmouti, Mai Mohamed Medhat Abdelhalim. "Engineering a genetically relevant zebrafish model of human uveal melanoma." Thesis, University of Manchester, 2016. https://www.research.manchester.ac.uk/portal/en/theses/engineering-a-genetically-relevant-zebrafish-model-of-human-uveal-melanoma(6bc6f02b-8d80-4e6e-bc5b-72fab73b0788).html.
Full textMant, Christine Andrea. "Background studies relevant to human papillomavirus type 16 vaccine development." Thesis, King's College London (University of London), 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.408673.
Full textArber, Charles. "Generation of disease-relevant neurons from human pluripotent stem cells." Thesis, Imperial College London, 2013. http://hdl.handle.net/10044/1/14670.
Full textFuchs, Talia Leah. "Investigation and Implementation of Clinically Relevant Prognostic Biomarkers in Human Malignancies." Thesis, The University of Sydney, 2022. https://hdl.handle.net/2123/27973.
Full textWagner, Andreas [Verfasser]. "Identification of process-relevant kinases in human amniotic production cells / Andreas Wagner." Ulm : Universität Ulm, 2019. http://d-nb.info/1192823958/34.
Full textHaas, Simone [Verfasser], Anton [Akademischer Betreuer] Cathomen, and Stephan [Akademischer Betreuer] Ehl. "Tracing the specificity of CRISPR-Cas nucleases in clinically relevant human cells." Freiburg : Universität, 2019. http://nbn-resolving.de/urn:nbn:de:bsz:25-freidok-1514002.
Full textHaas, Simone Alexandra [Verfasser], Anton [Akademischer Betreuer] Cathomen, and Stephan [Akademischer Betreuer] Ehl. "Tracing the specificity of CRISPR-Cas nucleases in clinically relevant human cells." Freiburg : Universität, 2019. http://d-nb.info/1233965611/34.
Full textLangenbrunner, Mary R., and Jamie Branam Kridler. "The Challenge of On-Line Human Services Courses: Keeping it Real and Relevant." Digital Commons @ East Tennessee State University, 2011. https://dc.etsu.edu/etsu-works/3481.
Full textWu, Celina. "Dual agonist-antagonist functions of FTY720 influence neuroinflammation-relevant responses in human astrocytes." Thesis, McGill University, 2012. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=110720.
Full textLes astrocytes sont les cellules gliales les plus abondantes du système nerveux central (SNC). Leur grande expression en filaments intermédiaires, la protéine acide fibrillaire gliale (GFAP), est une caractéristique permettant leur identification. Les astrocytes sont d'importants contributeurs aux événements biochimiques du SNC et jouent un rôle clé dans le processus de régulation des dommages et de la guérison du SNC. Sous des conditions d'inflammation chronique, tel la Sclérose en Plaques (SP), les astrocytes subissent des changements pathophysiologiques causant l'astrogliose (Liberto, Albrecht et al. 2004; Sidoryk-Wegrzynowicz, Wegrzynowicz et al. 2011). Ce mécanisme de cicatrisation est commun dans la SP et un nouvel agent thérapeutique, FTY720 (fingolimod, Gilenya™) démontre des effets protecteurs du SNC en prévenant l'évolution de l'astrogliose. (Choi, Gardell et al. 2011). FTY720 est un agent thérapeutique récemment approuvé pour traiter la SP. Il est administré oralement et a la capacité d'accéder au SNC. Une fois en place dans ce système, cet agent entre en contact direct avec le récepteur sphingosine-1-phosphate (S1PR) sur les astrocytes. Les réponses des astrocytes en réaction aux signaux générés par ce récepteur sont reliées à la pathologie de la SP. Cette thèse examine les signaux engendrés par FTY720 ainsi que ses fonctions sur les astrocytes humains primaires. Nous avons utilisé des astrocytes isolés à partir de SNC humains fœtaux pour examiner les réponses neuro-inflammatoires générées par l'administration quotidienne de FTY720. FTY720 agit initialement comme un agoniste en activant le récepteur S1P, mais il agit également comme un antagoniste en causant l'internalisation et la dégradation de ce récepteur. Nous avons examiné ces deux phénomènes de façon à savoir s'ils agissent en concert. Nous affirmons qu'un récepteur internalisé par FTY720 continue de générer des signaux pour une période de temps prolongée (heures). Une addition simple de FTY720 désensibilise l'astrocyte, pour une période de >24h, au signal de phosphorylation de ERK (pERK) qui est généré par le récepteur extracellulaire. Cette période réfractaire du signal de transduction de pERK fût maintenue dans les astrocytes traités quotidiennement avec FTY720, sinon le signal pERK reparaît 72 heures après le traitement initial. De plus, la désensibilisation du récepteur fût reliée à l'absence de réponse proliférative induite par le ligand naturel sphingosine-1-phosphate (S1P). Nous avons aussi démontré que le traitement quotidien des astrocytes avec FTY720 atténue la capacité de IL-1β à activer les voies moléculaires sensibles au calcium. Le traitement quotidien avec FTY720 n'inhibe pas les signaux de pERK lorsque les astrocytes sont stimulés à l'aide de sérum, ni la sécrétion de IL-6 ou de IP-10 lorsqu'ils sont stimulés avec IL-1β. Nos résultats suggèrent que l'exposition quotidienne à FTY720 agit comme un antagoniste aux stimuli extérieur (tel le ligand naturel S1P) ainsi qu'un agoniste lorsque le récepteur est internalisé (inhibe la mobilisation du calcium lorsqu'exposé à IL-1β).
Kraus, Emma [Verfasser]. "Identification of small molecule inhibitors of clinically relevant human polyomavirus infections / Emma Kraus." Hamburg : Staats- und Universitätsbibliothek Hamburg Carl von Ossietzky, 2019. http://d-nb.info/1240835515/34.
Full textStovall, Olin Scott. "Accounting for Human Resources: Implications for Theory and Practice." Thesis, University of North Texas, 2001. https://digital.library.unt.edu/ark:/67531/metadc3026/.
Full textAyad, Nancy B. "Effects of Antidepressants on Human Mesenchymal Stem Cell Differentiation on Clinically Relevant Titanium Surfaces." VCU Scholars Compass, 2016. http://scholarscompass.vcu.edu/etd/4477.
Full textMiranda, Benjamin H. "Development of a novel, clinically-relevant model for investigating factors that stimulate human hair growth." Thesis, University of Bradford, 2011. http://hdl.handle.net/10454/5731.
Full textSrikanth, Priya. "Schizophrenia-Relevant DISC1 Interruption Alters Wnt Signaling and Cell Fate in Human iPSC-Derived Neurons." Thesis, Harvard University, 2015. http://nrs.harvard.edu/urn-3:HUL.InstRepos:23845068.
Full textMedical Sciences
Kong, Meardey. "Culturally Relevant Practices: A Case Study of NGOs Working in Cambodia to Combat Human Trafficking." Thesis, The University of Arizona, 2013. http://hdl.handle.net/10150/297629.
Full textAl, Tafif Abdullah. "PREDICTION OF HUMAN SYSTEMIC, BIOLOGICALLY RELEVANT PHARMACOKINETIC PROPERTIES BASED ON PHYSICOCHEMICAL PROPERTIES OF CALCIUM CHANNEL BLOCKERS." VCU Scholars Compass, 2012. http://scholarscompass.vcu.edu/etd/2868.
Full textHamid, Umar Imran. "Use of clinically relevant human models to test novel therapies for the acute respiratory distress syndrome." Thesis, Queen's University Belfast, 2015. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.695362.
Full textKia, Richard. "Novel approaches using human induced pluripotent stem cells and microRNAs in the development of relevant human hepatocyte models for drug-induced liver injury." Thesis, University of Liverpool, 2014. http://livrepository.liverpool.ac.uk/2010059/.
Full textYoung, Ada. "Clinically relevant conventional dose of ionizing radiation enhances tumour cell migration in human breast cancer cell lines." Thesis, University of British Columbia, 2017. http://hdl.handle.net/2429/61247.
Full textMedicine, Faculty of
Pathology and Laboratory Medicine, Department of
Graduate
Hald, Rikke. "Generation and characterisation of a naive human antibody phage display library : a resource for clinically relevant reagents /." Cph. : Department of Pharmacology, The Danish University of Pharmaceutical Sciences, 2004. http://www.dfh.dk/phd/defences/rikkehald.htm.
Full textScholz, Diana [Verfasser]. "Regulation of Alzheimer’s disease-relevant protein processing in human neurons of the LUHMES cell line / Diana Scholz." Konstanz : Bibliothek der Universität Konstanz, 2011. http://d-nb.info/1028742843/34.
Full textVarghese, Christy Susan [Verfasser], and Martin [Akademischer Betreuer] Müller. "Biological Characterization of the Diagnostically Relevant Human Papillomavirus 16 E1C Transcript / Christy Susan Varghese ; Betreuer: Martin Müller." Heidelberg : Universitätsbibliothek Heidelberg, 2020. http://d-nb.info/1204321841/34.
Full textRubasha, Herbert. "Appreciating diversity : is the doctrine of margin of appreciation as applied in the European Court of Human Rights relevant in the African human rights system?" Diss., University of Pretoria, 2006. http://hdl.handle.net/2263/1228.
Full textPrepared under the supervision of Prof. Gilles Cistac at the Faculty of Law, Universidade Eduardo Mondlane, Maputo, Mocambique
Thesis (LLM (Human Rights and Democratisation in Africa)) -- University of Pretoria, 2006.
http://www.chr.up.ac.za/academic_pro/llm1/dissertations.html
Centre for Human Rights
LLM
Sofy, Laura. "Establishing the Relevant Standards of Human Rights Protection under Dublin Regulation - A question of more than responsibility determination?" Thesis, Uppsala universitet, Juridiska institutionen, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-295234.
Full textBoriachek, Kseniia. "Next generation of human cancer diagnostics: Nanomaterial-based electrochemical sensors for clinically relevant exosomes and exosomal biomarkers analysis." Thesis, Griffith University, 2018. http://hdl.handle.net/10072/380570.
Full textThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Environment and Sc
Science, Environment, Engineering and Technology
Full Text
Gudmundsson, Anders. "Studies on particle size-selective sampling of aerosols relevant for deposition in the human airways and onto the eyes." Lund : Dept. of Industrial Engineering, Division of Working Environment, Lund Institute of Technology, Lund University, 1995. http://books.google.com/books?id=XWZtAAAAMAAJ.
Full textThomas, Andrew G. "On the plasticity of human mating strategies : experimental evidence for mating strategy change in response to evolutionarily relevant stimuli." Thesis, Swansea University, 2015. https://cronfa.swan.ac.uk/Record/cronfa41184.
Full textRademan, Janet Ellen. "The identification of contextually relevant health and well-being information needs for the youth through human-centered co-design." Thesis, Cape Peninsula University of Technology, 2015. http://hdl.handle.net/20.500.11838/2409.
Full textAvailable health and well-being information is limited in communities with insufficient health care resources. This affects the community negatively on multiple levels in which the health and well-being needs of individuals are not satisfied. This research project explored the impact of human centred co-design, using tools such as health and well-being needs questionnaires including a health needs assessment as well as a quality of life scale. The aim was making accurate health and well-being information more accessible to the youth. The target group was Durbanville youth aged between 14 and 18 years. The sample included different ages ( = 15), races (79% White, 21% Coloured) and near equal gender distribution (55% female, 45% male). The sample (N = 33) was comprised of three groups: Group A, B, and C. A Human-Centered Design (HCD) framework was used during the project referring to the following three steps: Hear, Create, and Deliver. During the Hear phase, stories and inspiration from the participants were gathered. Group A (n = 10) completed a health and well-being information needs questionnaire. Group B (n = 15) discussed the topic, and created affinity diagrams. This was how the health and well-being status and information needs were established. During the Create phase; frameworks, opportunities, solutions, and prototypes were developed by the participants. Group B co-designed the concept prototype: a possible mobile application solution for practical access to health and well-being information. Group C (n = 8) provided feedback and input on the concept prototype and created storyboards to visually display scenarios in which they would use the mobile application. This step produced a youth-friendly health and well-being information service concept prototype. During the Deliver phase, the relevant health and well-being information solution was established as a youth-friendly health and well-being mobile application: WeHelp. Also, group A, B, and C were introduced to a similar existing resource named MobieG. Thus, the present study contributed directly to the participants’ health and well-being awareness. The research provided significant health and well-being insights. For example, the youth of Durbanville revealed extremely low scores on the emotional well-being domain. The data collected makes it possible for future researchers to create a practical, youth-friendly, health and well-being information service.
Klein, Sebastian [Verfasser], and Elmar [Akademischer Betreuer] Heinzle. "Towards the assessment of human-relevant repeated dose toxicity using an in vitro systems toxicological approach / Sebastian Klein ; Betreuer: Elmar Heinzle." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2017. http://d-nb.info/1152094742/34.
Full textKlein, Sebastian Verfasser], and Elmar [Akademischer Betreuer] [Heinzle. "Towards the assessment of human-relevant repeated dose toxicity using an in vitro systems toxicological approach / Sebastian Klein ; Betreuer: Elmar Heinzle." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:291-scidok-ds-269362.
Full textHey, Christina K. Mae. "Situating Critical Indigenous Worldview within Western Academic Traditions: Place-Based and Culturally-relevant Science Education for Human Empowerment and Environmental Sustainability." Diss., Virginia Tech, 2017. http://hdl.handle.net/10919/77577.
Full textPh. D.
Harrop, Ceri. "Biochemical, biophysical and network properties of mucins and mucus gels produced by human bronchial epithelial cells in response to disease-relevant mediators." Thesis, University of Manchester, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.532253.
Full textBadri, Prajakta. "PREDICTION OF HUMAN SYSTEMIC, BIOLOGICALLY RELEVANT PHARMACOKINETIC (PK) PROPERTIES BASED ON QUANTITATIVE STRUCTURE PHARMACOKINETIC RELATIONSHIPS (QSPKR) AND INTERSPECIES PHARMACOKINETIC ALLOMETRIC SCALING (PK-AS)." VCU Scholars Compass, 2010. http://scholarscompass.vcu.edu/etd/124.
Full textLambrianides, A. "An investigation of the molecular basis of interactions between human monoclonal antibodies and antigens that are clinically relevant in systemic lupus erythematosus and the Antiphospholipid Syndrome." Thesis, University College London (University of London), 2007. http://discovery.ucl.ac.uk/1444917/.
Full textLisovsky, Irene. "Emergence of Nelfinavir and Lopinavir resistance relative to a clinically relevant human immunodeficiency virus type-1 single nucleotide polymorphism at position 36 in protease enzyme «in vitro»." Thesis, McGill University, 2010. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=86996.
Full textThe 36th amino acid in the viral protease (PR) of B subtypes is Methionine (M), while non-B subtypes code for Isoleucine (I). I at position 36 is associated with PI resistance in subtype B HIV-1; therefore, we sought to investigate the effect of this single nucleotide polymorphism on emergence of resistance mutations and PI susceptibility in various HIV-1 subtypes in vitro. Our results indicate that the effect of this single nucleotide polymorphism appears to be subtype specific and PI specific.
Les différences génétiques (polymorphismes) entre les différents sous-types du VIH-1, soit B et non-B, sont bien documentées. Toutefois, les antirétroviraux incluant les inhibiteurs de la protéase, ont été conçus de façon structurelle et fonctionnelle en utilisant les informations obtenues à partir du sous-type B. L'impact des polymorphismes des différents sous-types du VIH-1 sur l'efficacité des antirétroviraux doit être évalué dû à l'émergence des infections de sous-types non-B dans les pays développés ainsi qu'avec l'arrivée de la thérapie antirétrovirale dans les pays en voie de développement.
Le 36e acide aminé de la protéase virale de sous-type B du VIH-1 est une méthionine. Cependant, cet acide aminé est remplacé par une isoleucine dans les sous-types non-B. La présence d'une isoleucine à la position 36 entraîne une résistance du VIH-1 sous-type B pour les inhibiteurs de la protéase. Nous avons examiné l'effet de ce polymorphisme sur les mutations de résistance dans les différents sous-types de VIH in vitro. Nos résultats indiquent que l'effet de ce polymorphisme serait différent selon le sous-type.
Gottipati, Gopichand. "PREDICTION OF HUMAN SYSTEMIC, BIOLOGICALLY RELEVANT PHARMACOKINETIC (PK) PROPERTIES USING QUANTITATIVE STRUCTURE PHARMACOKINETIC RELATIONSHIPS (QSPKR) AND INTERSPECIES PHARMACOKINETIC ALLOMETRIC SCALING (PK-AS) APPROACHES FOR FOUR DIFFERENT PHARMACOLOGICAL CLASSES OF COMPOUNDS." VCU Scholars Compass, 2014. http://scholarscompass.vcu.edu/etd/3525.
Full textLeung, Man Ching. "Identification of human hair follicle antigens targeted in the presumptive autoimmune hair follicle disorder Alopecia Areata and their potential functional relevance In Vitro. Methods development for isolation and identification of Alopecia Areata-relevant human hair follicle antigens using a proteomics approach and their functional assessment using an Ex Vivo hair follicle organ culture model." Thesis, University of Bradford, 2008. http://hdl.handle.net/10454/4330.
Full textKATIA, CAPITANI. "Genome editing for clinically relevant mutations in genetic diseases and cancer." Doctoral thesis, Università di Siena, 2022. http://hdl.handle.net/11365/1211914.
Full textOwor, Maureen. "Making international sentencing relevant in the domestic context : lessons from Uganda." Thesis, University of Bristol, 2009. http://hdl.handle.net/1983/3d520048-dba7-4393-ba22-664923c079c3.
Full textBourgart, Etienne. "Métabolisme cutané et biomarqueurs d'exposition aux mélanges complexes d'hydrocarbures aromatiques polycycliques A realistic human skin model to study benzo[a]pyrene cutaneous absorption in order to determine the most relevant biomarker for carcinogenic exposure Solar simulated light exposure alters metabolization and genotoxicity induced by benzo[a]pyrene in human skin Influence of exposure dose, complex mixture, and ultraviolet radiation on skin absorption and bioactivation of polycyclic aromatic hydrocarbons." Thesis, Université Grenoble Alpes (ComUE), 2019. http://www.theses.fr/2019GREAS026.
Full textPolycyclic aromatic hydrocarbons (PAH) are ubiquitous carcinogens emitted as complex mixtures whose composition depends on emission sources. Because of their abundance and genotoxicity, PAHs are classified as priority substances, to which people can be exposed via dermal absorption during occupational activities. Biomonitoring takes into account skin absorption as well as inhalation and allows the identification of hazardous exposure situations. To assess Benzo[a]pyrene (B[a]P) exposure, which is classified as carcinogenic to human, quantification of 3-hydroxybenzo[a]pyrene (3-OHB[a]P) and (±)trans-anti-B[a]P-tetrol (B[a]P-tetrol) was recently proposed. This PhD thesis aimed at studying the skin absorption and metabolism of B[a]P and PAH mixtures to improve the understanding of their genotoxicity and develop relevant biomarker for health risk assessment. The first part of this work consisted in developing a simple and realistic skin model from human skin explants. Further to the development of adequate extraction and analytical methods, cutaneous toxicokinetic and metabolism from low B[a]P doses were studied. B[a]P skin penetration and metabolism were inversely proportional to applied dose. Nevertheless, metabolic pathways are impacted differently. While 3-OHB[a]P production formed during detoxification was dose-dependent, the formation of B[a]P-tetrol, resulting from the hydrolysis of B[a]P ultimate carcinogenic metabolite, saturates rapidly. Therefore, B[a]P-tetrol is the most relevant biomarker for estimating B[a]P carcinogenic risk. In addition, unmetabolized B[a]P poorly diffused through skin indicating that B[a]P toxicity is mainly local. The second part of this work consisted of a literature review focusing on 7 other carcinogenic PAH biotransformation to identify 16 marketed metabolites of interest. In fine, GC-MS/MS analysis was developed for 10 previously identified metabolic intermediates that are either involved in bioactivation or detoxification pathways of 5 PAH. Urinary quantification of those new biomarkers should improve the biomonitoring of populations to carcinogenic PAH. Finally, we evaluated the impact of synthetic or industrial mixtures (coal tar pitch and petroleum coke extracts) composition at different doses on carcinogenic PAH skin absorption and metabolism combined or not with ultraviolet radiations (UVR). PAH penetration diminished when mixture complexity and dose increased. While UVR increased PAH penetration when industrial complex mixtures were applied, no effect was observed on pure B[a]P or synthetic mixtures. PAH bioactivation decreased with mixtures and UVR, inducing unmetabolized PAH accumulation in the skin which may delay the occurrence of genotoxic effects. Similarly to B[a]P, other carcinogenic PAH toxicity seems to be mainly local and depends on skin exposure scenario. This work underlines the importance of mixtures study owing to more complex chemical interactions than simple additive effects
Alosaimi, Shatha Mobarak. "Leveraging Whole Genome Sequences to Compare Mutational Mechanism and Identify Medically Relevant Variation in African versus Non-African Descend Populations." Master's thesis, Faculty of Health Sciences, 2020. http://hdl.handle.net/11427/32191.
Full textSiegle, Greg Jeremy. "Cognitive and physiological aspects of attention to personally relevant negative information in depression /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 1999. http://wwwlib.umi.com/cr/ucsd/fullcit?p9935457.
Full textConradie, Johannes David. "Investigation of myostatin and relevant regulators during muscle regeneration after an acute bout of eccentric exercise." Thesis, Stellenbosch : Stellenbosch University, 2014. http://hdl.handle.net/10019.1/95947.
Full textENGLISH ABSTRACT: The aim of this study was to investigate the powerful muscle regulator, myostatin, and its regulators in response to an acute bout of plyometric training. The participants were recruited and screened by characterization by means of isometric force production tests, baseline blood creatine kinase levels and VO2 max results. The selected individuals (n=15) were subjected to a baseline muscle biopsy for comparative purposes. The study made use of plyometric jumping, as source of eccentric exercise, to serve as an exercise intervention after which muscle biopsies (4 hours post and 24 hours post) and blood draw (4 hours post, 24 hours post and 48 hours post) samples were taken. Maximal voluntary isometric contractions of the knee extensors were also measured immediately after the exercise protocol and after 1 week recovery. Creatine kinase (CK) analysis on the serum samples was used to conclude muscle damage. The muscle biopsy samples were used for protein quantification (Western blot) and gene expression assessment (semi-quantitative and real-time PCR). The results showed decreased force production immediately after eccentric exercise (p < 0.05), while returning back to baseline values at 1 week post exercise and CK results showed a significant increases at 4 hours (p<0.05), 24 hours (p<0.001) and 48 hours (p<0.01) after exercise. There were no significant differences in myostatin precursor protein (43 kDa), phosphorylated Smad2,3, Smad7 or activin receptor IIb in response to eccentric exercise. However, the follistatin protein was increased at both 4 hours and 24 hours after exercise (p<0.01). RNA analysis of the extracellular matrix (ECM) protein, decorin, revealed the existence of the splice variants A1 and A2 in human skeletal muscle. The RT-PCR analysis (n=4) of these variants showed no significant difference when comparing pre- to post-exercise. The decorin core protein was also investigated by means of antibody probing and results revealed the need for ABC chondroitinase enzyme treatment before immunoblotting of human skeletal muscle samples. The results concerning knee extensor force reduction and circulating creatine kinase showed the effectiveness of plyometric jumping in producing skeletal muscle damage in the lower limbs of unfit individuals, unaccustomed to eccentric exercise. In conclusion, myostatin, and its associated signalling cascade, are not activated in early muscle regeneration, but follistatin is increased during this phase possibly aiding and initiating the muscle repair process. Future studies: Variants of decorin are expressed in human skeletal muscle, increasing the complexity that should be taken into account in studies concerning the regulation of decorin in a human model. Investigation into myostatin protein at different post-translational levels needs more clarification. Published methods and materials used in different laboratories are not consistent and investigators should attempt to standardise protocols in order to compare results between studies more effectively. Of importance, these results show that the myostatin at protein level report different results compared to mRNA analysis and that more investigation into myostatin regulatory factors, with special reference to follistatin and decorin, is needed in future human models.
AFRIKAANSE OPSOMMING: Die doel van hierdie studie was om die kragtige spiere reguleerder, miostatin, en sy reguleerders in reaksie op 'n akute aanval van pliometriese spronge te ondersoek. Die deelnemers is gewerf en gekeur deur karakterisering deur middel van isometriese krag produksie toetse, basislyn bloed kreatien kinase vlakke en VO2maks resultate. Die geselekteerde individue (N = 15) is onderhewig aan 'n basislyn spierbiopsie vir vergelykende doeleindes. Die studie het gebruik gemaak van pliometriese spronge (essentriese spier aksie) as die oefening intervensie waarna spierbiopsie (4 uur na en 24 uur na) en bloed (4 uur na, 24 uur na en 48 uur na) monsters geneem is. Isometriese kontraksies van die knieverlengers is ook gemeet onmiddellik na die oefening protokol en na 1 week se herstel. Kreatine kinase (KK) ontleding van die serum monsters is gebruik om spierskade aftelei. Die spierbiopsie monsters was gebruik vir proteïen kwantifisering (Western klad) en die assessering van geen uitdrukking (semi-kwantitatiewe en real-time PCR). Die resultate het gewys dat krag produksie afgeneem het onmiddellik na essentriese oefening (p <0.05), terwyl dit terugkeer na die oorspronklike waardes 1 week na oefening en KK resultate toon 'n beduidende toename by 4 uur (p <0,05), 24 uur (p <0,001) en 48 uur (p <0,01) na oefening. Daar was geen betekenisvolle verskille in Miostatien voorloper proteïen (43 kDa), gefosforileerde Smad2,3, Smad7 of Activin reseptoor IIb in reaksie op essentriese oefening. Dit is egter die follistatien proteïen wat verhoog by beide 4 uur en 24 uur na oefening (p <0,01). RNS ontleding van die ekstrasellulêre matriks (ESM) proteïen, decorin, het die bestaan van die splitsing variante A1 en A2 in menslike skeletspier, aan die lig gebring. Die RT-PCR analise (n = 4) van hierdie variante het geen betekenisvolle verskille getoon wanneer voor met na-oefening vergelyk is. Die decorin kern proteïen is ook ondersoek deur middel van teenliggaam afhanklike metodes en resultate het die behoefte aan ABC chondroitinase ensiem behandeling voor immunokladding van menslike skeletspier monsters gesteun. Die resultate aangaande knieverlenger krag vermindering en sirkuleerende kreatien kinase het die doeltreffendheid van pliometriese spronge in die vervaardiging van skeletspier skade in die onderste ledemate van individue ongewoond aan essentriese oefening verseker. Ten slotte, Miostatien, en sy verwante sein kaskade, is nie geaktiveer vroeg in spier herstelling, maar follistatien is tydens hierdie fase verhoog en help moontlik met die aanvang van die spier herstel. Toekomstige studies: variante van decorin word uitgedruk in menslike skeletspier, wat die kompleksiteit aangaande decorin verhoog en dit is iets wat in ag geneem moet word in studies wat handel oor die regulering van decorin in mens modelle. Ondersoek na miostatien proteïen op verskillende na-translasie vlakke moet meer duidelikheid verkry. Gepubliseer metodes en materiaal wat gebruik word in verskillende laboratoriums is nie konsekwent en ondersoekbeamptes moet probeer om protokolle te standaardiseer sodat resultate van studies meer effektief kan vergelyk word. Van belang is, die resultate wys dat miostatien op proteïen vlak verskillende resultate vertoon in vergelyking met boodskapper-RNS ontleding en dat meer ondersoek na miostatien regulerende faktore, met spesiale verwysing na follistatien en decorin, nodig is in toekomstige menslike modelle.
Gavaza, Takayedzwa. "Culturally-relevant augmented user interfaces for illiterate and semi-literate users." Thesis, Rhodes University, 2012. http://hdl.handle.net/10962/d1006679.
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Orendurff, Michael S. "Dynamic foot and ankle characteristics in functionally relevant gait performance in those with and without a pathology." Thesis, University of Roehampton, 2012. https://pure.roehampton.ac.uk/portal/en/studentthesis/dynamic-foot-and-ankle-characteristics-in-functionally-relevant-gait-performance-in-those-with-and-without-a-pathology(abd2dc9a-26a9-47fd-afcc-edee49360c64).html.
Full textSinisalo, Johanna. "Interactions between humans and dogs : Neurobiological factors relevant for the treatment of exhaustion-related disorders." Thesis, Högskolan i Skövde, Institutionen för biovetenskap, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-11436.
Full textPersson, Eva. "Drug Dissolution under Physiologically Relevant Conditions In Vitro and In Vivo." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Universitetsbiblioteket [distributör], 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7195.
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