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Journal articles on the topic "HspSO"
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Bala, Nurudeen Muhammad, and Suhailan Bin Safei. "A Hybrid Harmony Search and Particle Swarm Optimization Algorithm (HSPSO) for Testing Non-functional Properties in Software System." Statistics, Optimization & Information Computing 10, no. 3 (January 9, 2021): 968–82. http://dx.doi.org/10.19139/soic-2310-5070-1039.
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An important aspect of improving software system is testing. However, it is time demanding and sometimeslabour intensive if done manually. In this paper, we developed an automatic search-based approach for testing the nonfunctional properties of a software system using hybrid harmony search and particle swarm optimization algorithms. The approach birthed a new algorithm named HSPSO, which is proposed based on the strength of HS over Genetic algorithm (GA) in terms of less adjustable parameters, quick convergence and smooth implementation. On the other hand, we propose the PSO to complement the drawback of HS in terms of time consumption problem. Besides, we used four programs for the comparative efficiency analysis of the proposed algorithm in relation to competing algorithms based on average branch coverage and execution time. The results from the analysis showed that the HSPSO algorithm was able to achieve 100% average coverage with a fewer number of generated test cases and under limited execution time.
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Xu, Ji, Hong Zhou, Yanjun Fang, and Lan Liu. "Data-Driven Approach for the Short-Term Business Climate Forecasting Based on Power Consumption." Wireless Communications and Mobile Computing 2022 (April 27, 2022): 1–11. http://dx.doi.org/10.1155/2022/4037053.
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With the fast development of intelligent data-mining technologies, some advanced artificial intelligence approaches are widely developed and employed to help the decision-making of enterprises and government. The application of advanced and intelligent approaches successfully helps the enterprises and government find out the valuable information hidden in the massive economic data. This study presents a novel data-driven approach to forecast the short-term business climate using the electric power consumption data of large enterprises. In addition, the climate conditions, interactions between different industries, the business cycle, and some other related variables are also considered and included in the developed forecasting model. To be specific, the business climate prediction model based on support vector machine (SVM) is proposed firstly, and the human-simulated particle swarm optimization algorithm (HSPSO) in our previous work is employed to optimize the parameters of the developed forecasting model. Secondly, a novel power-consumption-based business climate index (BCI) system is developed and comprehensively analyzed. The developed BCI system that contains the index for each separate industry (BCI-I), the index for tertiary industry (BCI-T), the index for secondary industry (BCI-S), and the index for the entire province (BCI-P) is proposed. In addition, the developed BCI system is employed to normalize the output of SVM-based forecasting model to directly indicate the business climate, which is very important to the decision-making of enterprises and government under the background of smart cities. Finally, the real data of Guangdong province in China, including the gross output values (GOV) and detailed power consumptions of more than 38000 enterprises, are employed to test the proposed approach. Experimental results show that the GOV of each industry and the whole society predicted by HSPSO-SVM matches the real data well. Moreover, the predicted BCI can directly indicate the business climate in advance, which is of great value for economic-decision and policy-making of both enterprises and government.
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Banu, P. K. Nizar, and S. Andrews. "Harmony Search PSO Clustering for Tumor and Cancer Gene Expression Dataset." International Journal of Swarm Intelligence Research 5, no. 3 (July 2014): 1–21. http://dx.doi.org/10.4018/ijsir.2014070101.
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Enormous quantity of gene expression data from diverse data sources are accumulated due to the modern advancement in microarray technology that leads to major computational challenges. The foremost step towards addressing this challenge is to cluster genes which reveal hidden gene expression patterns and natural structures to find the interesting patterns from the underlying data that in turn helps in disease diagnosis and drug development. Particle Swarm Optimization (PSO) technique is extensively used for many practical applications but fails in finding the initial seeds to generate clusters and thus reduces the clustering accuracy. One of the meta-heuristic optimization algorithms called Harmony Search is free from divergence and helps to find out the near-global optimal solutions by searching the entire solution space. This paper proposes a novel Harmony Search Particle Swarm Optimization (HSPSO) clustering algorithm and is applied for Brain Tumor, Colon Cancer, Leukemia Cancer and Lung Cancer gene expression datasets for clustering. Experimental results show that the proposed algorithm produces clusters with better compactness and accuracy, in comparison with K-means clustering, PSO clustering (swarm clustering) and Fuzzy PSO clustering.
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Kaneda, F., and P. G. Kwiat. "High-efficiency single-photon generation via large-scale active time multiplexing." Science Advances 5, no. 10 (October 2019): eaaw8586. http://dx.doi.org/10.1126/sciadv.aaw8586.
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Deterministic generation of single- and multiphoton states is a key requirement for large-scale optical quantum information and communication applications. While heralded single-photon sources (HSPSs) using nonlinear optical processes have enabled proof-of-principle demonstrations in this area of research, they are not scalable as their probabilistic nature severely limits their generation efficiency. We overcome this limitation by demonstrating a substantial improvement in HSPS efficiency via large-scale time multiplexing. Using an ultra-low loss, adjustable optical delay to multiplex 40 conventional HSPS photon generation processes into each operation cycle, we have observed a factor of 9.7(5) enhancement in efficiency, yielding a 66.7(24)% probability of collecting a single photon with high indistinguishability (90%) into a single-mode fiber per cycle. We also experimentally investigate the trade-off between a high single-photon probability and unwanted multiphoton emission. Upgrading our time-multiplexed source with state-of-the-art HSPS and single-photon detector technologies will enable the generation of >30 coincident photons with unprecedented efficiency.
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Tower, John. "Hsps and aging." Trends in Endocrinology & Metabolism 20, no. 5 (July 2009): 216–22. http://dx.doi.org/10.1016/j.tem.2008.12.005.
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Chen, Wei, Pengmian Feng, Tao Liu, and Dianchuan Jin. "Recent Advances in Machine Learning Methods for Predicting Heat Shock Proteins." Current Drug Metabolism 20, no. 3 (May 22, 2019): 224–28. http://dx.doi.org/10.2174/1389200219666181031105916.
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Background:As molecular chaperones, Heat Shock Proteins (HSPs) not only play key roles in protein folding and maintaining protein stabilities, but are also linked with multiple kinds of diseases. Therefore, HSPs have been regarded as the focus of drug design. Since HSPs from different families play distinct functions, accurately classifying the families of HSPs is the key step to clearly understand their biological functions. In contrast to laborintensive and cost-ineffective experimental methods, computational classification of HSP families has emerged to be an alternative approach.Methods:We reviewed the paper that described the existing datasets of HSPs and the representative computational approaches developed for the identification and classification of HSPs.Results:The two benchmark datasets of HSPs, namely HSPIR and sHSPdb were introduced, which provided invaluable resources for computationally identifying HSPs. The gold standard dataset and sequence encoding schemes for building computational methods of classifying HSPs were also introduced. The three representative web-servers for identifying HSPs and their families were described.Conclusion:The existing machine learning methods for identifying the different families of HSPs indeed yielded quite encouraging results and did play a role in promoting the research on HSPs. However, the number of HSPs with known structures is very limited. Therefore, determining the structure of the HSPs is also urgent, which will be helpful in revealing their functions.
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Baszczynski, Chris L. "Immunochemical analysis of heat-shock protein synthesis in maize (Zea mays L.)." Canadian Journal of Genetics and Cytology 28, no. 6 (December 1, 1986): 1076–87. http://dx.doi.org/10.1139/g86-151.
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Polyclonal antibodies to 18-kilodalton (kDa) heat-shock proteins (HSPs) and to the high molecular weight (73 000 – 89 000) HSPs from 5- day-old maize plumules have been produced in rabbits. The antisera to high molecular weight HSPs show minor cross-reactivity to proteins of similar molecular mass in not heat-shocked tissues, while antisera to 18-kDa HSPs react only with this 18-kDa HSP class. HSPs of similar molecular mass and isoelectric points in maize plumules, mesocotyls, radicles, and young leaves also have similar antigenic determinants based on positive reactions with antisera to plumule HSPs. Among 13 maize inbreds and genetic stocks tested, differences were noted in the amount of immunoprecipitable 18-kDa HSPs. Antisera to maize plumule HSPs also showed cross-reactivity with similar-sized HSPs from pea epicotyls and soybean hypocotyls but not with HSPs from several animal tissues.Key words: polyclonal antibodies, maize, heat-shock.
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Song, Xueming, Zhiqiang Chen, Chunbo Wu, and Shiguang Zhao. "Abrogating HSP Response Augments Cell Death Induced by As2O3 in Glioma Cell Lines." Canadian Journal of Neurological Sciences / Journal Canadien des Sciences Neurologiques 37, no. 4 (July 2010): 504–11. http://dx.doi.org/10.1017/s0317167100010544.
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Objectives:We previously reported that Arsenic trioxide (ATO) can inhibit glioma growth both in vitro and in vivo. While the use of ATO alone for solid tumor treatment sometimes was found to be ineffective which may be due to the protective pathways including heat shock proteins (HSPs) response induced by ATO. In this study, we modified HSPs expression to investigate whether HSPs had some effect on ATO induced glioma cell death.Methods:Trypan bule exclusion assay, mitochondrial membrane potential (MMP) Assay, and SubG1 detection were used to evaluate cell viability and western-blot was employed to detect HSPs and some apoptosis markers expression induced by ATO. Heat pre-treatment, HSPs inhibitor, or Heat Shock factor-1 (HSF1) knockdown by SiRNA was employed to modify HSPs levels.Results:It was showed that KNK437 (HSPs inhibitor) or HSF1 knockdown significantly enhanced cell death, MMP disruption, JNK phosphorylation and caspase-3 cleavage induced by ATO, which was accompanied by abrogation of HSPs induction, while heat pre-treatment with clear HSPs induction had strong protection on the effects mentioned above.Conclusion:Those data suggested that HSPs play protective roles on ATO induced cell death in glioma. Inhibition of HSPs may have a synergistic effect with ATO on glioma treatment.
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Vierling, Elizabeth. "MOLECULAR ANALYSIS OF HEAT STRESS PROTEINS IN HIGHER PLANTS." HortScience 25, no. 9 (September 1990): 1175e—1175. http://dx.doi.org/10.21273/hortsci.25.9.1175e.
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When plants experience high temperature stress, they respond by synthesizing a discrete set of proteins called heat shock proteins (HSPs). This response is not unique to plants, but is observed in all other eukaryotes. It is now known that the HSPs are evolutionarily conserved proteins, and furthermore, that HSPs function not only during stress, but also during normal growth and development. My laboratory has characterized several of the major groups of HSPs in higher plants. We have cloned genes encoding plant HSP70 proteins and low molecular weight (LMW) HSPs (17-23 kDa). Using this information we have investigated the expression of HSPs both in the field, and under laboratory conditions which mimic field situations. We have determined the temperature limits for expression of HSPs in vegetative tissues, and have also found that HSPs are frequently produced in plant reproductive structures, even in the absence of stress. As a first step toward understanding HSP function, we have characterized the intracellular localization of HSPs. Results show that there are unique HSPs in the cytoplasm, chloroplast and endomembrane system. These ubiquitous proteins appear to play essential roles in many cellular processes.
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Rodríguez-Iturbe, B., and RJ Johnson. "Heat shock proteins and cardiovascular disease." Physiology International 105, no. 1 (March 2018): 19–37. http://dx.doi.org/10.1556/2060.105.2018.1.4.
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The development of stress drives a host of biological responses that include the overproduction of a family of proteins named heat shock proteins (HSPs), because they were initially studied after heat exposure. HSPs are evolutionarily preserved proteins with a high degree of interspecies homology. HSPs are intracellular proteins that also have extracellular expression. The primary role of HSPs is to protect cell function by preventing irreversible protein damage and facilitating molecular traffic through intracellular pathways. However, in addition to their chaperone role, HSPs are immunodominant molecules that stimulate natural as well as disease-related immune reactivity. The latter may be a consequence of molecular mimicry, generating cross-reactivity between human HSPs and the HSPs of infectious agents. Autoimmune reactivity driven by HSPs could also be the result of enhancement of the immune response to peptides generated during cellular injury and of their role in the delivery of peptides to the major histocompatibility complex in antigen-presenting cells. In humans, HSPs have been found to participate in the pathogenesis of a large number of diseases. This review is focused on the role of HSPs in atherosclerosis and essential hypertension.
Dissertations / Theses on the topic "HspSO"
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Foglia, Antonio. "Design, synthesis and biological evaluation of new anticancer and/or anti-inflammatory agents." Doctoral thesis, Universita degli studi di Salerno, 2017. http://hdl.handle.net/10556/2484.
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2014 - 2015One of the main goal of modern medicinal chemistry is the development of new agents able to modulate biological targets involved in inflammation and cancer processes. In this context, my PhD project was focused on the exploration and structural optimization of various chemical moieties able to interfere with two targets involved in both processes. In particular, two biological targets were selected: Heat shock protein 90 (Hsp90) and microsomal Prostaglandin E2 Synthase-1 (mPGES-1). The results obtained can be divided into two sections in accordance with the target of interest. a) Exploration and structural optimization of DHPM core in order to guide the synthesis of new and more potent Hsp90 C-terminal inhibitors. Hsp90 is a molecular chaperone involved in the maturation and stabilization of a wide range of client proteins that play a crucial role in the development, survival and proliferation of cancer cells. In the literature there are several compounds capable of inhibiting this molecular chaperone. The most part of these compounds inhibit the protein through modulation of the N-terminal domain. However, this type of modulation involves a well-known heat shock response, a cytoprotective mechanism that as a final result leads to the increase of cytosolic levels of heat shock proteins with consequent cell survival. Therefore, the modulation of C-terminal domain of Hsp90 represents a better strategy for the development of new antitumor agents, since, they do not induce heat shock response. In an attempt to discover new modulators of the C-terminal domain of Hsp90 and taking into account the structure of the first synthetic inhibitor of this domain, a 3,4-dyhidropyrimidin-2(1H)-one (DHPM) derivative, two more generations of DHPM derivatives have been synthesized. Relatively to the second generation of DHPM derivatives, the synthesis was focused on the influence of the chemical functionalization of aromatic ring at C4 position of DHPM core, while the third generation has been designed with the aim to functionalize the C2 position of the core. The exploration and optimization processes of DHPM core led to the identification of novel and more potent inhibitors of the C-terminal domain of Hsp90. b) Identification of new mPGES-1 inhibitors. mPGES-1 is an inducible enzyme that catalyzes the terminal step of the biosynthesis of PGE2 from the PGH2 precursor. The inhibition of this enzyme appears to be a promising strategy for the identification of novel anti-inflammatory agents, because, the use of selective inhibitors would allow to overcome the classical side effects of traditional anti-inflammatory drugs. Moreover, mPGES-1 is overexpressed in a wide variety of human cancers and for this reason it has emerged as an attractive biological target for anticancer drug discovery. In order to identify new molecular platforms able to interact with the target protein three collections of compounds (carbazoles, biaryl compounds and 5-pyrazolones) were synthesized. Biological evaluation revealed the identification of five biaryl compounds (60-64) as new chemical entities that inhibit mPGES-1 activity with promising IC50 values (ranging 0.18-1.64 μM). [edited by author]
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Bourrelle-Langlois, Maxime. "Caractérisation des petites protéines de stess/small heat shock proteins du cyanophage S-ShM2 (HspSP-ShM2) et de son hôte Synechococcus WH7803 (HspS-WH7803)." Master's thesis, Université Laval, 2016. http://hdl.handle.net/20.500.11794/26763.
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Les petites protéines de stress/Small heat shock proteins (sHsps) sont des chaperons moléculaires ATP-indépendants ubiquitaires retrouvées chez les procaryotes et eucaryotes. Elles sont dynamiques structurellement et la majorité d’entre elles possèdent la capacité de former de gros complexes oligomériques. Également, elles protègent les cellules du stress protéotoxique causé par divers facteurs de stress abiotiques en prévenant l’agrégation des protéines dénaturées et en promouvant leur repliement par les chaperons moléculaires ATP dépendants tels que Hsp70/DnaK. Récemment, la présence de gènes de sHsp (HspSP-ShM2) chez des virus marins et plus précisément chez des cyanophages infectant le genre Synechococcus sp. et Prochlorococcus sp ont été caractérisés in silico. Au niveau de sa séquence, la sHsp de 18 kDa de Synechococcus sp. montre un domaine alpha crystallin de 92 acides aminés hautement conservé au sein des sHsps, une région C-terminale contenant le motif CAM canonique de type (L-X-I/L/V) et une région N-terminale relativement courte. Nous avons établi grâce à la chromatographie par exclusion stérique (SEC) et le système de Fast Protein Liquid Chromatography (FPLC) sa capacité à former des complexes oligomériques de haut poids moléculaires (600 kDa et 200kDa). De plus, nous avons démontré qu’elle prévient l’agrégation de la citrate synthase, la malate dehydrogenase et la luciférase en condition de stress thermique suggérant qu’elle possède une faible spécificité et un large spectre de protéines clientes/substrats. La prévention complète de l’agrégation a été obtenue à différents ratios (sHsp:substrat) selon le substrat, ce qui indique qu’il y aurait possiblement des interactions différentes et uniques avec chacun d’eux. Nous avons ensuite mis en évidence la formation d’hétéro-oligomères stables et solubles entre HspSP-ShM2 et son substrat dans les mêmes conditions, ce qui est en accord avec les caractéristiques des sHsps en général. Quant à elle, la sHsp de la cyanobactérie Synechococcus WH7803 (HspS-WH7803) possède un poids moléculaire de 15 kDa et montre une capacité à former des tétramères (60 kDa) sur essai de SEC par FPLC en présence de Triton™X-100 pour le maintien de sa solubilité. Contrairement à HspS-ShM2, HspS-WH7803 ne démontre aucune activité protectrice sur l’agrégation des substrats mentionnés précédement à différents ratios molaires. Finalement, des analyses par SEC/FPLC suggèrent la formation de complexes hétéro-oligomériques entre HspSP-ShM2 et celle de son hôte, HspS-WH7803 de Synechococcus WH7803. Cette interaction entre les sHsps pourrait soit optimiser ou inhiber l’activité de chaperon moléculaire et la réponse au stress de son hôte dans le but de favoriser le cycle viral.Small heat shock proteins (sHsps) are ubiquitous ATP-independent molecular chaperones found in prokaryotes and eukaryotes. They are structurally dynamic and most of them have the ability to form large oligomeric complexes and to protect cells from proteotoxic stresses by preventing aggregation of non-native proteins and promoting their refolding via ATP-dependent chaperones such as Hsp70/DnaK. Recently, the presence of a sHsp gene (HspSP-ShM2) in marine viruses has been reported using bioinformatics tools. More precisely, the gene has been found in cyanophages infecting cyanobacteria of the genre Synechococcus sp. and Prochlorococcus sp. The Synechococcus phage sHSP has a MW of 18 kDa and shows the highly conserved core alpha crystalline domain of 92 amino acids and relatively short N- and C-terminal arms, the later containing the classical CAM domain (L-X-I/L/V). We established its oligomeric profile using a size exclusion chromatography (SEC) and Fast Protein Liquid Chromatography (FPLC) system and demonstrated its ability to form large oligomeric complexes in native conditions (600 kDa and 200kDa). Furthermore, we report its capacity to prevent the aggregation of citrate synthase, malate dehydrogenase and luciferase suggesting that it has a weak specificity and wide range of protein substrates. The complete prevention of aggregation was achieved at different ratios (sHsp:substrate) depending on the substrate indicating that the sHSP may have different and unique interactions with each of its clients. We also showed the formation of a stable and soluble hetero-oligomeric complex of the phage sHSP and its substrates under heat stress, which is in accordance with the characteristics of sHSP in general. The cyanobacteria Synechococcus WH7803 15 kDa sHSP (HspS-WH7803) shows the ability to form tetramers in the presence of Triton™X-100 for the maintenance of its solubility using the SEC/FPLC method. For its capability to prevent the aggregation of different substrates, HspS-WH7803 demonstrates no chaperon like activity in all the assays and molar ratios used. Finally, SEC/FPLC results indicate the possible formation of a hetero-oligomeric complex between the sHSP of the phage and the one from its host Synechococcus WH7803 (HspS-WH7803). This interaction could either optimize the chaperone activity and the stress response of its host or inhibit the host sHSP to facilitate the viral life cycle.
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Gaborit, Nadège. "Intérêt de la vectorisation et/ou de l’induction des protéines de stress dans les modèles expérimentaux de pathologies ostéoarticulaires : Validation de l’électroporation biphasique." Thesis, Nancy 1, 2008. http://www.theses.fr/2008NAN10127/document.
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Lors des pathologies articulaires, les chondrocytes sont soumis à différents processus qui concourent à la dégradation du cartilage articulaire soit en entraînant la mort par apoptose (nonrenouvellement des constituants de la matrice) soit par l’activation de protéases détruisant les constituants matriciels. Le potentiel chondroprotecteur des protéines de stress (Hsp70 / Hsp27) lors des pathologies dégénératives ayant été démontré, nous avons souhaité évaluer l'intérêt thérapeutique de l’induction de ces protéines par un inhibiteur réversible du protéasome (MG132) dans un modèle expérimental : modèle par section du ligament croisé antérieur (SLCA). Au cours de cette étude, nous avons du évaluer un nouveau système de vectorisation afin de surexprimer les protéines de stress dans le cartilage articulaire. Cette technique (électroporation biphasique) repose sur la dissociation des impulsions perméabilisantes et électrophorétiques. Nous avons donc développé des plasmides nous permettant de surexprimer ces protéines, fusionnées à une protéine traceur, facilitant ainsi leur détection dans le tissu ciblé. Puis, nous avons évalué l’innocuité et l’efficacité des impulsions sur des tissus sains et pathologiques (dégénératifs et inflammatoires). Nous avons constaté que cette technique, en plus de limiter les altérations du tissu articulaire ou les phénomènes de dissémination, offrait des propriétés d’adressage tissulaire de part la multiplicité des combinaisons d’impulsions (nombre, fréquence et intensité). Enfin, les effets de l’induction (MG132) des Hsps sur un modèle physiopathologique (SLCA) ont été évalués et nous avons pu montrer une diminution de la sévérité des atteintes articulaires, tant au niveau du cartilage que de la membrane synoviale. Cette molécule non seulement permet à la fois de renforcer la résistance des chondrocytes aux atteintes mais également de limiter l’amplitude de la réponse inflammatoire qui participe à la dégradationDuring articular diseases, chondrocytes suffer different mechanisms which take part in the degradation of the cartilage, either by generating cell death by apoptosis (without renewal of extracellular matrix components), or by protease activation which destroy matrix components. Based on the cytoprotective potential of Heat Shock proteins (Hsp70 and Hsp27) during degenerative diseases, we evaluated the therapeutic interest of these proteins induced by a transient proteasome inhibitor (MG132), in an experimental model, by transection of the anterior cruciate ligament (ACLT). During this study, we have evaluated a new electroporation system to overexpress HSPs in articular cartilage. This technique is based on two sets of electric pulses, wich have two roles, to permeabilize the target and to transport DNA across the permeabilized membrane. We have developed expression vectors to generate a fusion protein (Hsps linked to GFP). Effectively, GFP permit to detect simply the fusion protein in the targeted tissue by fluorescence. Besides, we have evaluated safety and efficiency of electric pulses on healthy and alterated tissues (degenerative and inflammatory). We have reported that this technique could limit articular tissue damages and, moreover, could offer the ability to target more specifically this tissue. Indeed, this apparatus allows a great number of electrics pulses combinations (number, frequency, intensity). Finally, the effects of the induction via MG132 of Hsps in a physiopathological ACLT model, have been evaluated and we have shown a decrease of severity of joint lesions, in cartilage and synovial tissues. This molecule has the advantage to reinforce the resistance of chondrocytes at stressful stimuli and moreover, to limite the amplitude of inflammatory response which contribute to the magnification of extracellular matrix destruction
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Guzha, Delroy Tapiwa. "Investigating the biological roles of the HSPRO genes in Arabidopsis thaliana." Doctoral thesis, University of Cape Town, 2015. http://hdl.handle.net/11427/15503.
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As a consequence of an immobile lifestyle, plants have had to evolve appropriate perception mechanisms and responses to diverse environmental stresses. Stress can be the result of both biotic and abiotic agents and the ORTHOLOG OF SUGAR BEET HS1 PRO-1 (HSPRO 1) and HSPRO2 genes were previously shown to be induced in response to several stresses including infection with Pseudomonas syringae and drought stress in Arabidopsis thaliana. The aim of this study was to characterise the biological role(s) played by these proteins in Arabidopsis. Several bioinformatics approaches provided evidence that supported function of both genes in response to both biotic and abiotic stresses and identified potential regulatory elements that may drive HSPRO gene expression during stress responses. Accordingly, analysis of null hspro mutants revealed antagonistic functions of the two proteins in PAMP-triggered immunity to P. syringae infections of shoot tissues and osmotic stress tolerance in plant roots. HSPRO proteins have been shown to interact with a central integrator of stress and energy signalling, SUCROSE NON-FERMENTING-1-RELATED KINASE1 (SnRK1) and microarray analysis of the null mutants suggested potential roles in carbohydrate signalling. An array of energy responsive genes including a subset of SnRK1 targets were misregulated in hspro mutants under standard growth conditions supporting involvement of HSPRO in energy signalling. Mutant phenotype and gene expression analysis revealed that HSPRO2 may be of importance in energy perception as hspro2 seeds were hypersensitive to exogenous glucose during germination, and that perception and/or signalling of low energy status may require HSPRO2. Although HSPRO2 expression may be driven via perception of environmental stress cues, promoter-luciferase assays revealed a diurnal expression pattern of the gene that was driven by the circadian clock. However, phenotypic analysis did not reveal a requirement of HSPRO2 for normal clock modulation. Since stress perception typically causes fluctuations in energy levels, it is proposed that HSPRO genes are important for the integration of energy and stress signalling in an effort to maintain a homeostatic balance between coping with environmental stress and normal growth and development.
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Willis, Dean. "The role of heat shock proteins in models of acute inflammation." Thesis, Queen Mary, University of London, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.265943.
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Fritah, Sabrina. "Implications des histones deacetylases de I et II dans la réponse au stress." Université Joseph Fourier (Grenoble), 2008. http://www.theses.fr/2008GRE10270.
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En réponse à des stress environnementaux, la cellule met en place une réponse rapide et transitoire visant à assurer sa survie. Cette réponse se traduit par l'activation du facteur HSF1(Heat shock factor 1) qui induit l'expression des gènes codant pour des protéines de choc thermique (ou HSP). L'activation des gènes hsp s'accompagne de la répression de la plupart des autres gènes cellulaires. Si l'on connait assez bien aujourd'hui les mécanismes associés à l'activation des gènes de choc thermique, peu de données existent concernant les mécanismes mis en jeu dans l'inactivation globale. Nous avons engagé un travail visant à caractériser les modifications épigénétiques qui accompagnent cette répression, ainsi qu'à identifier les acteurs impliqués. Par des approches moléculaires et in situ nous avons montré que les HDACs (Histones Décaétylases) sont de nouveaux régulateurs de la réponse au stress. Le stress thermique induit une régulation fine de l'épigénome, notamment une déacétylation globale des histones de cœur, médiée par des HDACS de classe l, HDAC1 et 2. Au niveau du cytoplasme, les HDACs régulent également la réponse au stress. En effet, lors d'un stress protéotoxique, nous avons montré qu'HDAC6 joue un rôle indispensable dans l'initiation de cette réponse, en dissociant le facteur HSFl de ses régulateurs négatifs. En conséquence, HDAC6 joue un rôle dans l'induction des protéines HSPs en réponse à ce stress de type agrégats. En conclusion, en identifiant les HDACs comme de nouveaux facteurs de la réponse au stress, nos travaux permettent de mettre en lien entre deux cibles faisant l'objet de nombreux travaux en cancérologie: HSPs et HDACsLn response to environmental stress (heat shock, hypoxia, heavy metals exposure), cells have developed rapid and transitory mechanisms to protect themselves from the stress-induced damages. This stress response is characterized by the activation of HSF1 (Heat Shock Factor1), a key factor involved in the induction of the HSP (Heat Shock Proteins) encoded genes. Ln contrast toheat shock genes induction, most of the genome is repressed du ring stress. If the mechanisms involved in the activation of HSP genes have been investigated in details, less is known about the global repression of the genome. We started to investigate the epigenetic mechanisms that underline this genome repression and identify the molecular basis of this phenomenon. By molecular and in situ approaches, we showed that HDACs (Histone Deacetylases) are new regulators of stress response. Heat shock induces major epigenetic changes, specially a global deacetylation of core histones. We showed that class 1 HDAC, HDACl and HDAC2 mediates the heat-induced deacetylation. This event is regulated by HSF1, probably through its interaction with HDACl and HDAC2. Ln the cytoplasm, HDACS are also able to regulate stress response. Indeed, upon proteotoxic stress for example, proteasome inhibition, we showed that HDAC6 play a critical role in initiating the stress response. It mediates the dissociation of HSFl from its repressor complex and HDAC6 has an impact in HSP induction in response to stress. Ln conclusion, we identify HDACs as new important factors of stress response. Thanks to this work, we have linked two classes of proteins that are targeted by anti-cancer therapy: HSPs and HDACs
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Jacob, Tiago Rinaldi. "Expressão, regulação e funcionalidade de genes HSPs no dermatófito Trichophyton rubrum." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/17/17135/tde-15052014-105536/.
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O dermatófito Trichophyton rubrum é um fungo filamentoso, queratinofílico e antropofílico, sendo o principal agente etiológico de micoses cutâneas em humanos. Sua distribuição cosmopolita e seu acometimento grave em pacientes imunocomprometidos fazem dele um dos desafios a ser enfrentado pelos serviços de saúde pública mundial. As interações patógeno-hospedeiro envolvem diferentes processos relacionados com a degradação de queratina e com modificações metabólicas que permitem sua adesão e posterior penetração nos tecidos infectados. Essas alterações são importantes para o sucesso do processo infeccioso e envolvem mecanismos que modulam a expressão gênica, a secreção de proteínas específicas, a adaptação metabólica e as alterações no pH cutâneo, fundamentais para o estabelecimento da infecção. Dentre as proteínas que participam do processo de interação patógeno-hospedeiro estão as proteínas de choque térmico HSPs (Heat Shock Proteins), relacionadas aos mais diversificados processos celulares. Nesse sentido, a hipótese desse trabalho foi avaliar se os genes hsps de T. rubrum, bem como seu principal fator de transcrição (Hsf1), estão envolvidos nos processos de resposta a situações adversas e na interação com o microambiente hospedeiro, e se estes genes são modulados pelo fator de transcrição pacC, um regulador envolvido na sinalização do pH ambiente. Para tanto, a expressão dos genes hsps foi analisada em resposta ao cultivo de T. rubrum em diferentes meios de cultura, durante a exposição a antifúngicos, estresse térmico e interação com fragmentos de unha e pele humanas. O envolvimento da Hsp90 na modulação da expressão gênica, na suscetibilidade a antifúngicos e na interação de T. rubrum com fragmentos de unha humana foi avaliado utilizando-se um inibidor químico específico para esta proteína. Os resultados indicam uma expressão variável dentre os genes hsps e até mesmo dentro de cada família HSP, em resposta a cada condição ambiental ou interação a que o fungo foi exposto. Além disto, temos indício de que a expressão dos genes hsps seja modulada pelo fator de transcrição PacC, através da modulação do fator de transcrição Hsf1. Constatamos ainda, a influência de Hsp90 na susceptibilidade de T. rubrum às drogas Itraconazol e Micafungina, e no desenvolvimento de T. rubrum em fragmentos de unha humana. Esses resultados revelam a participação das proteínas HSPs em diversos aspectos do metabolismo de T. rubrum, e sugerem a participação de Hsp90 na patogenicicade e na suscetibilidade a drogas deste dermatófitoThe dermatophyte Trichophyton rubrum is a filamentous, keratinophilic, and anthrophophilic fungi, being the major etiologic agent of cutaneous mycoses in humans. Its cosmopolitan distribution and the severe infection in immunocompromised patients make it one of the challenges to be faced by public health agencies worldwide. Hostpathogen interactions involve different processes related to keratin degradation and metabolic changes that allow adhesion and subsequent penetration of the infected tissue. These changes are important to the success of the infectious process and involve mechanisms that modulate gene expression, secretion of specific proteins, and metabolic adaptation, and cutaneous pH changes, essential to the establishment of the infection. Among the proteins that participate in the host-pathogen interaction are the heat shoch proteins (HSPs), related to diverse cellular processes. Thus, the hypothesis of this work was to evaluate whether T. rubrum hsp genes, as well as their major transcription factor (Hsf1), are involved in the response to adverse situations and in the interaction with the host microenvironment, and if these genes are regulated by the transcription fator PacC, a regulator of the pH signaling pathway. The expression of the hsp genes was evaluated in response to the cultivation of T. rubrum in different culture medium, during exposure to antifungal drugs, heat stress, and interaction with human nail and skin. The involvement of T. rubrum Hsp90 in the modulation of gene expression, susceptibility to antifungal drugs, and interaction with human nails was evaluated by using a chemical inhibitor, specific to this protein. Our results indicate a variable expression of the hsp genes, even among members of the same HSP family, in response to each environmental condition or interaction, to which the fungus was exposed. Furthermore, we have evidence that the hsp gene expression is modulated by the PacC transcription factor, by modulating the expression of the Hsf1 coding gene. We also found that Hsp90 is involved in T. rubrum susceptibility to the drugs Itraconazole and Micafungin, and in the development of this dermatophyte in human nails. These results reveal the involvement of HSPs in several aspects of T. rubrum metabolism, suggesting a role for Hsp90 in the pathogenicity and drug susceptibility in this dermatophyte
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Monteiro, Janaína Munuera. "Imunolocalização das Heat Shock Proteins (HSPs) 60 e 70 na placenta bovina." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/10/10132/tde-27062006-105146/.
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As Heat Shock Proteins (HSPs) ou proteínas do choque térmico são encontradas em todas as células e são classificadas de acordo com seu peso molecular. Dentre elas encontram-se as de 27, 60, 70, 90 e 110 kDa, sendo as mais estudadas no contexto da reprodução as da família 60 e 70. Essas proteínas são ditas como chaperoninas, em razão do seu importante papel no dobramento e desdobramento de outras proteínas celulares sem alterar sua conformação final, e são expressas frente a qualquer tipo de estresse como calor, vírus, bactéria, hormônios, diferenciação celular, etc, e influenciam nas respostas imune inata e adquirida. A placenta também expressa essas proteínas, uma vez que é um órgão de intenso estresse e diferenciação celular durante toda a gestação. Nesse estudo, busca-se avaliar a expressão ou não dessas proteínas na placenta bovina e para isso foram utilizadas 30 amostras de diferentes animais em estágios distintos de gestação, fixadas em formol tamponado a 10% e processadas pela técnica de imuno-istoquímica. O mesmo numero de amostras foi também processado para a análise de imuno-microscopia eletrônica de transmissão pelas técnicas de \"freeze-substitution\" e marcação por pós-embebição. Na imuno-istoquímica, as HSPs 60 e 70 foram localizadas nos trofoblastos, epitélio materno e células binucleadas. A expressão da HSP 60 foi maior no início declinando no segundo e terceiro terço. Já a expressão da HSP 70 manteve-se praticamente constante, evidenciando a forte expressão dessa proteína durante todo o período. Na análise de imuno-microscopia eletrônica de transmissão, ambas as famílias foram localizadas nas células binucleadas (núcleo, citoplasma e vesículas) e epitélio materno (núcleo e citoplasma) em todos os terços gestacionais. O perfil das proteínas estudadas na placenta bovina foi diferente quando comparada à placenta humana, pois nesta última, a intensidade da expressão para a HSP 70 diminuiu com o decorrer da gestação enquanto para a HSP 60 foi constante durante todo a gestação. Provavelmente essas diferenças podem estar relacionadas ao fato dessas amostras terem sido coletadas de mulheres com gravidez interrompidas e também pelo tipo de placentação distinta. A bovinocultura de corte é de extrema importância para a econômica para o Brasil e se faz necessário o conhecimento de fatores que possam melhorar suas características reprodutivas. Dessa forma os resultados obtidos nesse estudo contribuirão certamente de subsídio para experimentos futuros sobre o papel das Heat Shock Proteins na placenta bovina.Heat Shock Proteins (HSP) can be found in any kind of cell. These proteins are classified according to their molecular weight and their known families include the HSP 27, 60, 70, 90 and 110 kDa. Among these, HSP 60 and 70 are the ones of interest in reproduction. They were known as chaperonines because of their capacity to fold and unfold other proteins into the cell, without changing their own conformation. They are expressed during several stress conditions likes virus and bacteria infections, hormones, heat, cellular differentiation, etc, and also take part signalizing for innate and acquired immune responses. Heat shock proteins are expressed in several tissues and organs, including the placenta. In this study we have evaluated the expression of these proteins in the bovine placenta, using thirty samples from different animais with distinct gestational periods, fixed in 10% formalin and processed for immunohistochemistry. The same numbers of samples were processed for immunoelectron microscopy using freeze-substitution and post embedding labeling techniques. The immunohistochemistry results show the expression of HSP 60 and 70 in trophoblasts, maternal epithelia and binucleated cells. The HSP 60 expression was higher in the beginning of gestation, becoming lower during the second and third trimester. Heat shock protein 70 expression were practically constant throughout the gestation. The immunoelectron microscopy analysis revealed that both HSP 60 and 70 were located in the cytoplasm and nucleio binucleated cells and maternal epithelia from the beginning to the end of pregnancy. The immunolocalization of HSP 60 and 70 in the bovine placenta were distinct from the ones found in studies on women, probably due to the differences of the placentation type and to the fact that those samples were collected from abnormal or discontinuous pregnancy. Beef production in Brazil is an important economical activity and studies to improve the bovine reproductive characteristics are necessary and must be expended, therefore our results certainly contributes for further studies on HSP function during pregnancy in this species.
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Champagne, Marie-Josée. "Caractérisation de la relation possible entre les protéines de stress (HSPs) et l'hypertension." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0002/NQ39728.pdf.
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Coelho, Danielle Letícia Martins. "Estresse hídrico com diferentes osmóticos em sementes de feijão e expressão diferencial de proteínas durante a germinação." Universidade do Oeste Paulista, 2008. http://bdtd.unoeste.br:8080/tede/handle/tede/349.
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Previous issue date: 2008-08-21Snap Beans (Phaseolus vulgaris L) are highly valuable nutritionally, and it is cultivated by small, medium and big farmers. It represents a significant parcel of Brazilian economy concerning to the society. Culture emergency is a critical point in the production process and it is affected mainly by the water deficiency at this phase. The objective of this work was to simulate water deficiency in the germination beginning at the laboratory on seeds of snap beans ´Pérola´, using mannitol, CaCl2, MgCl2 and NaCl as osmotic in the potential of 0; -0.3; -0.6; -0.9 and -1.2MPa calculated with the aim of Van´t Hoff s equation and to evaluate the electrophorectical protein patterns of total soluble proteins by SDS-PAGE. Germination, vigour classification, roots and shoot dry weight and differential protein expression response was evaluated as parameters. The experimental design was completely randomized. Data was analysed by F test (ANOVA) and polynomial regression for the osmotic potential for each parameter evaluated. Banding pattern was evaluated by gel image. Simulation of deficiency, in laboratory, allowed the perception of the stress originated by NaCl in all parameter evaluated, validating the harsh of the NaCl and the lack of expression of low molecular weight proteins in this osmotic. 110 and 30kDa proteins were indicative of water stress, but not of salinity.
O feijão (Phaseolus vulgaris L) é uma cultura de grande expressão alimentícia. A emergência da cultura é dependente de água, sendo considerada a fase mais crítica. O objetivo deste foi simular deficiência de água no início da germinação em laboratório, em sementes de feijão Pérola , utilizando-se: manitol, CaCl2, MgCl2 e NaCl em potenciais de 0; -0,3; -0,6; -0,9 e -1,2MPa estabelecidos pela equação de Van´t Hoff e avaliar o perfil eletroforético de proteínas totais solúveis através de SDS-PAGE. Foram avaliados: germinação, classificação de vigor, massa seca de raiz e de parte aérea e resposta diferencial de expressão de proteínas. O delineamento experimental foi inteiramente casualizado. Os dados foram analisados através da aplicação do teste F, para análise de variância, regressão polinomial para os níveis de potencial osmóticos para cada uma das variáveis fisiológicas estudadas. O bandeamento eletroforético foi avaliado visualmente através da imagem dos géis. A simulação do estresse permitiu avaliar a drasticidade do NaCl em todos os parâmetros avaliados e a ausência de proteínas de baixo peso molecular neste osmótico. As proteínas de 110 e 30kDa foram indicativas de estresse hídrico, mas não do salino.
Books on the topic "HspSO"
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Berkeley. Office for History of Science and Technology University of California. Historical studies in the physical and biological sciences: HSPS. Berkeley: University of California Press, 1986.
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Calderwood, Stuart Keith, Ayesha Murshid, and Thiago J. Borges, eds. HSPs - Ambiguous Mediators of Immunity. Frontiers Media SA, 2017. http://dx.doi.org/10.3389/978-2-88945-152-4.
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RPV, droner & andre platforme: OPTRONK/HSPS. [København: Faggruppe OPTRONIK, 1989.
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Newport, Natalie. Navigating All Those Assholes : : A Guidebook for Empaths and HSPs. Independently Published, 2017.
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Newport, Natalie. Navigating All Those A**holes : : A Guidebook for Empaths and HSPs. Independently Published, 2017.
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Hill, Silvia. Highly Sensitive People: What You Need to Know about HSPs and Their Gifts. EH Jolen, 2022.
Find full textBook chapters on the topic "HspSO"
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Harr, Jeffrey N., Philip F. Stahel, Phillip D. Levy, Antoine Vieillard-Baron, Yang Xue, Muhammad N. Iqbal, Jeffrey Chan, et al. "HSPs." In Encyclopedia of Intensive Care Medicine, 1152. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-00418-6_1713.
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Tomita, Misao, and Koki Shimoji. "Heterosegmental SCPs (HSPs)." In Evoked Spinal Cord Potentials, 82–89. Tokyo: Springer Japan, 2006. http://dx.doi.org/10.1007/4-431-30901-2_8.
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Gearhart, Christopher C. "Highly Sensitive Person Scale (HSPS)." In The Sourcebook of Listening Research, 299–305. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2017. http://dx.doi.org/10.1002/9781119102991.ch28.
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Rui, Zhiqing, Jingzheng Wu, Yanjie Shao, Tianyue Luo, Mutian Yang, Yanjun Wu, and Bin Wu. "PassEye: Sniffing Your Password from HTTP Sessions by Deep Neural Network." In Communications in Computer and Information Science, 3–15. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-33-4922-3_1.
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AbstractPasswords are the most widely used method for user authentication in HTTP websites. Password sniffing attacks are considered a common way to steal password. However, most existing methods have many deficiencies in versatility and automation, such as manual analysis, keyword matching, regular expression and SniffPass. In this paper, to better describe the problem, we propose a HTTP Sessions Password Sniffing (HSPS) attack model which is more suitable in HTTP environment. Furthermore, we propose PassEye, a novel deep neural networkbased implementation of HSPS attack. PassEye is a binary neural network classifier that learns features from the HTTP sessions and identifies Password Authentication Session (PAS). We collected 979,681 HTTP sessions from the HTTP and HTTPS websites for training the binary classifier. The results show that PassEye is effective in sniffing the passwords with an accuracy of 99.38%. In addition, several measures are provided to prevent HSPS attacks in the end.
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Fecek, Ronald J., Subhara Raveendran, Manoj Chelvanambi, and Walter J. Storkus. "Inhibition of HSPs for Enhanced Immunity." In Heat Shock Proteins in the Immune System, 157–80. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-69042-1_9.
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Gonçalves, Conrado C., and Carlos H. I. Ramos. "Molecular Chaperones and HSPs in Sugarcane and Eucalyptus." In Heat Shock Proteins and Plants, 245–82. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-46340-7_13.
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Haslbeck, Martin, Sevil Weinkauf, and Johannes Buchner. "Regulation of the Chaperone Function of Small Hsps." In Heat Shock Proteins, 155–78. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-16077-1_6.
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Kim, Jong Youl, Jong Eun Lee, and Midori A. Yenari. "Neuroprotection of Heat Shock Proteins (HSPs) in Brain Ischemia." In Translational Medicine Research, 383–95. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-5804-2_17.
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Herbert, Kate Reed, Afshin Samali, and Adrienne Gorman. "The Role of Hsps in Neuronal Differentiation and Development." In Heat Shock Proteins in Neural Cells, 25–37. New York, NY: Springer New York, 2009. http://dx.doi.org/10.1007/978-0-387-39954-6_3.
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Moreno, Andrés Rodríguez, Óscar Torreño Tirado, and Oswaldo Trelles Salazar. "Out of Core Computation of HSPs for Large Biological Sequences." In Advances in Computational Intelligence, 189–99. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-38682-4_22.
Full textConference papers on the topic "HspSO"
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Dogru, Nuran. "Increasing the Mode-Locking Range of HSPS." In Frontiers in Optics. Washington, D.C.: OSA, 2006. http://dx.doi.org/10.1364/fio.2006.jsua29.
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Song, Alfred S., and Kenneth R. Diller. "Modeling Heat Shock Protein Expression While Wearing a Therapeutic Heat Wrap." In ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-192823.
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Hyperthermia mediated repair of injured tissues is attributed to increased blood perfusion and mass transport. A complimentary mechanism of healing may exist where heat shock proteins (HSPs) are over expressed due to local hyperthermia. HSPs are able to stabilize the tertiary structure of nascent proteins and to help re-fold denatured proteins. In this report, we created a temperature model that characterizes the temperature distribution in the dermis, subcutaneous fat, and muscle tissues due to the application a heat source as a commercially marketed heat wrap. The temperature evolution due to the heat wrap was calculated by applying the finite difference version of Pennes’ bioheat equation to the back of a subject consisting of a composite, semi-infinite system geometry. The temperature model included the factors of metabolic heat generation and blood perfusion. The HSP expression was modeled via interpolation of experimental constitutive data. The results show that the over expression of HSPs in the target tissue area likely contribute to the efficacy of the heat wrap.
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Dogru, Nuran. "Grating parameters effect on actively mode-locked HSPS." In LEOS 2009 -22nd Annuall Meeting of the IEEE Lasers and Electro-Optics Society (LEO). IEEE, 2009. http://dx.doi.org/10.1109/leos.2009.5343173.
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Braga, Vinícius Lopes, Wladimir Bocca Vieira de Rezende Pinto, Bruno de Mattos Lombardi Badia, José Marcos Vieira de Albuquerque Filho, Igor Braga Farias, Paulo Victor Sgobbi de Souza, and Acary Souza Bulle Oliveira. "Spastic paraplegia type 73: expanding phenotype of the first two Brazilian families." In XIII Congresso Paulista de Neurologia. Zeppelini Editorial e Comunicação, 2021. http://dx.doi.org/10.5327/1516-3180.552.
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Context: Hereditary spastic paraplegias (HSPs) represent an expanding group of neurodegenerative diseases characterized mainly by progressive spastic paraparesis of the lower limbs. More than 80 different genetic loci have been associated with HSPs. In 2015, heterozygous pathogenic variants in the CPT1C gene were first associated with SPG73, not yet described in Brazilian patients. Objective: We present clinical, neuroimaging and genetic features of three Brazilian patients with SPG73. Cases reports: We report one male and two female patients, age range 36 to 78 years old. Case 1 presented with a 4-year-history of spasticity, predominantly crural tetraplegia, bladder incontinence, dysphagia and dysphonia. Family history disclosed a sister with epilepsy. Whole-exome sequencing (WES) disclosed a heterozygosis variant c.863G>A (p.Arg288His) in exon 9 of the CPT1C. Cases 2 and 3 are first degree relatives (mother and son). Both presented with long-standing slowly progressive spastic paraplegia. Case 3 presented bladder incontinence, constipation, dysphagia and dysphonia at late stages. Cases 2 and 3 WES disclosed the heterozygosis variant c.196T>G (p.Phe66Val) in exon 4 of the CPT1C. Discussion: Previous literature described six patients from an Italian family with pure HSPs phenotype and the pathogenic variant c.109C>G (p.Arg3. 7Cys) in CPT1C gene. Another group described three patients associated with pure HSPs phenotype and the pathogenic variant (c.226C>T) in the CPT1C gene. All previous reported cases had benign clinical course and bulbar involvement was not described before. One of our cases presented with a de novo variant and rapidly progressive motor and bulbar compromise. Conclusion: our cases expand the current knowledge about SPG73, including a rapidly progressive phenotype with bulbar involvement and cognitive compromise at late stages of disease course.
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Chung, Eunna, and Marissa Nichole Rylander. "Effects of Growth Factors and Stress Conditioning on the Induction of Heat Shock Proteins and Osteogenesis." In ASME 2009 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2009. http://dx.doi.org/10.1115/sbc2009-206662.
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Tissue engineering is an emerging field that focuses on development of methods for repairing and regenerating damaged or diseased tissue. Successful development of engineered tissues is often limited by insufficient cellular proliferation and insufficient formation of extracellular matrix. To induce effective bone regeneration, many research groups have investigated the cellular response and capability for tissue regeneration associated with bioreactor conditions and addition of growth factors [1]. Bioreactors in tissue engineering have been developed to expose cells to a similar stress environment as found within the body or induce elevated stress levels for potential induction of specific cellular responses associated with tissue regeneration. Native bone encounters a diverse array of dynamic stresses such as shear, tensile, and compression daily. Stress conditioning protocols in the form of thermal or tensile stress have been shown to induce up-regulation of molecular chaperones called heat shock proteins (HSPs) and bone-related proteins like MMP13 (matrix metallopeptidase 13) [2] and OPG (osteoprotegerin) [3, 4]. HSPs have important roles in enhancing cell proliferation and collagen synthesis. Osteogenic growth factors such as TGF-β1 (transforming growth factor beta 1) and BMP-2 (bone morphogenetic protein 2) are related to bone remodeling and osteogenesis as well as HSP induction [5]. Therefore, identification of effective preconditioning using growth factors and stress protocols that enhance HSP expression could substantially advance development of bone regeneration. The purpose of this research was to identify preconditioning protocols using osteogenic growth factors and tensile stress applied through a bioreactor system to enhance expression of HSPs and bone-related proteins while minimizing cellular injury for ultimate use for bone regeneration.
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De Silvestre Perrucio, Ligia, and Jaime Amaya-Farfan. "EFEITO DO CONSUMO DE AMINOÁCIDOS NAS HEAT SHOCK PROTEINS (HSPS) EM RATOS." In XXIV Congresso de Iniciação Científica da UNICAMP - 2016. Campinas - SP, Brazil: Galoa, 2016. http://dx.doi.org/10.19146/pibic-2016-51284.
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Hosseini-Beheshti, Elham, Amina Zoubeidi, ka Mun Nip, Martin E. Gleave, and Emma S. (Tomlinson) Guns. "Abstract 4150: Exploration of the role of Hsps in exosome derived from prostate cancer cells." In Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1538-7445.am10-4150.
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Chung, Eunna, and Marissa Nichole Rylander. "Multi-Stress Conditioning Can Synergisticly Enhance Production of Osteogenic Markers and Heat Shock Proteins." In ASME 2010 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2010. http://dx.doi.org/10.1115/sbc2010-19511.
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Tissue regeneration can be enhanced by introduction of biochemical and mechanical cues. We investigated the effect of thermal and mechanical stress alone or in combination with growth factors (GFs) (BMP-2 and TGF-β1) on cell proliferation and induction of heat shock proteins and bone-related proteins by MC3T3-E1mouse preosteoblasts. Thermal and mechanical stress conditioning alone induced bone-related proteins such as osteocalcin (OCN), vascular endothelial growth factor (VEGF), osteoprotegerin (OPG), and osteopontin (OPN) and heat shock proteins (HSP27, HSP47, HSP70). Cell proliferation was increased by cyclic tension in combination with growth factors. Combined thermal and mechanical stress induced synergistic expression of HSPs and VEGF. Therefore, utilization of thermal and tensile stress conditioning can stimulate bone healing or regeneration.
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Havlik, Nico, and Michael Lutz. "Optimization of Tilting-Pad Journal Bearings for Integrally Geared Compressors." In ASME Turbo Expo 2015: Turbine Technical Conference and Exposition. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/gt2015-42483.
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Tilting-pad journal bearings (TPJBs) support highly loaded high speed rotors with high requirements on rotordynamic behavior. Typical applications therefore are integrally geared compressors, where the gear force of the high speed pinions (HSPs) is at least one magnitude higher than the gravity force. A continuously rising demand for increasing the overall efficiency of integrally geared compressors leads to a necessity to expand TPJBs operation limits. Allowable limits of actual bearings often result in limitations for thermodynamical compressor design. In addition, bearings generate 20% to 40% of the mechanical power losses in integrally geared compressors. Improvements in the bearing design have to be performed in order to meet the challenges of rising loads and speeds. This paper presents an optimization of TPJBs for integrally geared compressors to meet the further demand of higher operation limits.
Reports on the topic "HspSO"
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Miller, Gad, and Jeffrey F. Harper. Pollen fertility and the role of ROS and Ca signaling in heat stress tolerance. United States Department of Agriculture, January 2013. http://dx.doi.org/10.32747/2013.7598150.bard.
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The long-term goal of this research is to understand how pollen cope with stress, and identify genes that can be manipulated in crop plants to improve reproductive success during heat stress. The specific aims were to: 1) Compare heat stress dependent changes in gene expression between wild type pollen, and mutants in which pollen are heat sensitive (cngc16) or heat tolerant (apx2-1). 2) Compare cngc16 and apx2 mutants for differences in heat-stress triggered changes in ROS, cNMP, and Ca²⁺ transients. 3) Expand a mutant screen for pollen with increased or decreased thermo-tolerance. These aims were designed to provide novel and fundamental advances to our understanding of stress tolerance in pollen reproductive development, and enable research aimed at improving crop plants to be more productive under conditions of heat stress. Background: Each year crop yields are severely impacted by a variety of stress conditions, including heat, cold, drought, hypoxia, and salt. Reproductive development in flowering plants is highly sensitive to hot or cold temperatures, with even a single hot day or cold night sometimes being fatal to reproductive success. In many plants, pollen tube development and fertilization is often the weakest link. Current speculation about global climate change is that most agricultural regions will experience more extreme environmental fluctuations. With the human food supply largely dependent on seeds, it is critical that we consider ways to improve stress tolerance during fertilization. The heat stress response (HSR) has been intensively studied in vegetative tissues, but is poorly understood during reproductive development. A general paradigm is that HS is accompanied by increased production of reactive oxygen species (ROS) and induction of ROS-scavenging enzymes to protect cells from excess oxidative damage. The activation of the HSR has been linked to cytosolic Ca²⁺ signals, and transcriptional and translational responses, including the increased expression of heat shock proteins (HSPs) and antioxidative pathways. The focus of the proposed research was on two mutations, which have been discovered in a collaboration between the Harper and Miller labs, that either increase or decrease reproductive stress tolerance in a model plant, Arabidopsis thaliana (i.e., cngc16--cyclic nucleotide gated channel 16, apx2-1--ascorbate peroxidase 2,). Major conclusions, solutions, achievements. Using RNA-seq technology, the expression profiles of cngc16 and apx2 pollen grains were independently compared to wild type under favourable conditions and following HS. In comparison to a wild type HSR, there were 2,776 differences in the transcriptome response in cngc16 pollen, consistent with a model in which this heat-sensitive mutant fails to enact or maintain a normal wild-type HSR. In a comparison with apx2 pollen, there were 900 differences in the HSR. Some portion of these 900 differences might contribute to an improved HSR in apx2 pollen. Twenty-seven and 42 transcription factor changes, in cngc16 and apx2-1, respectively, were identified that could provide unique contributions to a pollen HSR. While we found that the functional HS-dependent reprogramming of the pollen transcriptome requires specific activity of CNGC16, we identified in apx2 specific activation of flavonol-biosynthesis pathway and auxin signalling that support a role in pollen thermotolerance. Results from this study have identified metabolic pathways and candidate genes of potential use in improving HS tolerance in pollen. Additionally, we developed new FACS-based methodology that can quantify the stress response for individual pollen in a high-throughput fashion. This technology is being adapted for biological screening of crop plant’s pollen to identify novel thermotolerance traits. Implications, both scientific and agricultural. This study has provided a reference data on the pollen HSR from a model plant, and supports a model that the HSR in pollen has many differences compared to vegetative cells. This provides an important foundation for understanding and improving the pollen HSR, and therefor contributes to the long-term goal of improving productivity in crop plants subjected to temperature stress conditions. A specific hypothesis that has emerged from this study is that pollen thermotolerance can be improved by increasing flavonol accumulation before or during a stress response. Efforts to test this hypothesis have been initiated, and if successful have the potential for application with major seed crops such as maize and rice.
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Blum, Abraham, Henry T. Nguyen, and N. Y. Klueva. The Genetics of Heat Shock Proteins in Wheat in Relation to Heat Tolerance and Yield. United States Department of Agriculture, August 1993. http://dx.doi.org/10.32747/1993.7568105.bard.
Full textAbstract:
Fifty six diverse spring wheat cultivars were evaluated for genetic variation and heritability for thermotolerance in terms of cell-membrane stability (CMS) and triphenyl tetrazolium chloride (TTC) reduction. The most divergent cultivars for thermotolerance (Danbata-tolerant and Nacozari-susceptible) were crossed to develop an F8 random onbred line (RIL) population. This population was evaluated for co-segragation in CMS, yield under heat stress and HSP accumulation. Further studies of thermotolerance in relations to HSP and the expression of heterosis for growth under heat stress were performed with F1 hybrids of wheat and their parental cultivars. CMS in 95 RILs ranged from 76.5% to 22.4% with 71.5% and 31.3% in Danbata and Nacozari, respectively. The population segregated with a normal distribution across the full range of the parental values. Yield and biomass under non-stress conditions during the normal winter season at Bet Dagan dit not differ between the two parental cultivar, but the range of segregation for these traits in 138 RILs was very high and distinctly transgressive with a CV of 35.3% and 42.4% among lines for biomass and yield, respectively. Mean biomass and yield of the population was reduced about twofold when grown under the hot summer conditions (irrigated) at Bet Dagan. Segregation for biomass and yield was decreased relative to the normal winter conditions with CV of 20.2% and 23.3% among lines for biomass and yield, respectively. However, contrary to non-stress conditions, the parental cultivars differed about twofold in biomass and yield under heat stress and the population segregated with normal distribution across the full range of this difference. CMS was highly and positively correlated across 79 RILs with biomass (r=0.62**) and yield (r=0.58**) under heat stress. No such correlation was obtained under the normal winter conditions. All RILs expressed a set of HSPs under heat shock (37oC for 2 h). No variation was detected among RILs in high molecular weight HSP isoforms and they were similar to the patterns of the parental cultivars. There was a surprisingly low variability in low molecular weight HSP isoforms. Only one low molecular weight and Nacozari-specific HSP isoform (belonging to HSP 16.9 family) appeared to segregate among all RILs, but it was not quantitatively correlated with any parameter of plant production under heat stress or with CMS in this population. It is concluded that this Danbata/Nacozari F8 RIL population co-segregated well for thermotolerance and yield under heat stress and that CMS could predict the relative productivity of lines under chronic heat stress. Regretfully this population did not express meaningful variability for HSP accumulation under heat shock and therefore no role could be seen for HSP in the heat tolerance of this population. In the study of seven F1 hybrids and their parent cultivars it was found that heterosis (superiority of the F1 over the best parent) for CMs was generally lower than that for growth under heat stress. Hybrids varied in the rate of heterosis for growth at normal (15o/25o) and at high (25o/35o) temperatures. In certain hybrids heterosis for growth significantly increased at high temperature as compared with normal temperature, suggesting temperature-dependent heterosis. Generally, under normal temperature, only limited qualitative variation was detected in the patterns of protein synthesis in four wheat hybrids and their parents. However, a singular protein (C47/5.88) was specifically expressed only in the most heterotic hybrid at normal temperature but not in its parent cultivars. Parental cultivars were significantly different in the sets of synthesized HSP at 37o. No qualitative changes in the patterns of protein expression under heat stress were correlated with heterosis. However, a quantitative increase in certain low molecular weight HSP (mainly H14/5.5 and H14.5.6, belonging to the HSP16.9 family) was positively associated with greater heterosis for growth at high temperature. None of these proteins were correlated with CMS across hybrids. These results support the concept of temperature-dependent heterosis for growth and a possible role for HSP 16.9 family in this respect. Finally, when all experiments are viewed together, it is encouraging to find that genetic variation in wheat yield under chronic heat stress is associated with and well predicted by CMS as an assay of thermotolerance. On the other hand the results for HSP are elusive. While very low genetic variation was expressed for HSP in the RIL population, a unique low molecular weight HSP (of the HSP 16.9 family) could be associated with temperature dependant heterosis for growth.