Dissertations / Theses on the topic 'HSC'
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SCARFÒ, REBECCA. "Precise characterization of hemogenic endothelial cells during human hematopoietic development." Doctoral thesis, Università Vita-Salute San Raffaele, 2022. http://hdl.handle.net/20.500.11768/128259.
Full textDurante lo sviluppo embrionale, le cellule del sangue emergono da un sottoinsieme di cellule endoteliali specializzate, denominate endotelio emogeno (HE), attraverso un processo noto come transizione endoteliale-ematopoietica (EHT). HE è una popolazione eterogenea che si trova in vari siti anatomici, tra cui il sacco vitellino (YS) e l'aorta-gonade-mesonefro (AGM). Comprende cellule che differiscono per il potenziale di sviluppo, definendo così programmi ematopoietici distinti. Nonostante la discendenza endoteliale delle cellule del sangue sia ben consolidata, l'identità di HE è ancora dibattuta. Una caratterizzazione più approfondita di HE è quindi essenziale per guidare gli sforzi nel derivare questa popolazione dalle cellule staminali pluripotenti umane (hPSC), un passaggio fondamentale per generare emoderivati terapeutici in vitro. Tuttavia, gli attuali marcatori noti utilizzati per isolare l'HE non sono sufficienti poiché arricchiscono anche le cellule arteriose associate. Per identificare specifici marcatori HE umani, abbiamo eseguito l'analisi trascrittomica di embrioni umani di 4-5 settimane, una fase di sviluppo caratterizzata da EHT attivo. Abbiamo identificato la codifica FCGR2B per il recettore Fc CD32, precedentemente associato ad altri endoteli specializzati, come gene arricchito nella popolazione ACE+CD34+ che contiene HE. Analisi funzionali ex vivo hanno confermato che il potenziale ematopoietico multilineare è altamente arricchito nelle cellule endoteliali CD32+ isolate dall'AGM e dall'YS di embrioni umani. Inoltre, CD32 è emerso come marcatore selettivo per HE derivato da hPSC in diversi programmi ematopoietici. Sorprendentemente, le nostre analisi hanno mostrato che la specificità del CD32 per le cellule con potenziale emogeno è superiore ad altri marcatori HE noti nelle colture ematopoietiche derivate da hPSC. Questi risultati forniscono un metodo semplice per isolare HE da embrioni umani e hPSC, consentendo la sua caratterizzazione molecolare e la generazione efficiente di cellule ematopoietiche in vitro.
Stein, Sebastian [Verfasser]. "HSC-Kantenbearbeitung von Blech / Sebastian Stein." Aachen : Shaker, 2010. http://d-nb.info/1081884592/34.
Full textZhou, Xuan. "RhoA GTPase Controls Cytokinesis and Programmed Necrosis of Hematopoietic Progenitors." University of Cincinnati / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1378197381.
Full textMeiklejohn, Stuart J. "The role of BMP signalling in HSC ontogeny." Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:305597a8-b8cb-42ff-88fd-34b3dd5bf39b.
Full textSIGHINOLFI, SILVIA. "INTRACELLULAR IRON OVERLOAD AFFECTS HSC METABOLISM BY IMPAIRING MITOCHONDRIAL FITNESS IN β-THALASSEMIA." Doctoral thesis, Università Vita-Salute San Raffaele, 2023. https://hdl.handle.net/20.500.11768/137019.
Full textL'attività e il metabolismo mitocondriali controllano in modo significativo la funzione e il destino delle cellule staminali ematopoietiche (HSC). Le HSC modificano lo stato metabolico in risposta a segnali di stress, come le specie reattive dell'ossigeno (ROS), che guidano l'ingresso delle HSC nel ciclo cellulare accompagnato da un aumento della fosforilazione ossidativa mitocondriale (OXPHOS) e della glicolisi. Tuttavia, l'eccessivo accumulo di ROS provoca il danno ossidativo degli organelli cellulari, compresi i mitocondri. Il ferro è una delle fonti di ROS e le HSC possono assorbire il ferro, ma si sa poco sugli effetti del ferro sul metabolismo delle HSC. Recentemente, abbiamo dimostrato una funzione alterata delle HSC nella β-talassemia (BThal), una condizione di sovraccarico sistemico di ferro (IO). Abbiamo anche osservato che l'eccesso di ferro riduce la capacità di supporto ematopoietica delle cellule stromali mesenchimali talassemiche. Tuttavia, non ci sono prove dell'effetto diretto del sovraccarico di ferro sulle HSC in BThal. Abbiamo ipotizzato che il sovraccarico di ferro e il conseguente stress ossidativo alterino il metabolismo e la funzione delle HSC. Abbiamo trovato un arricchimento positivo dei geni dell'omeostasi del ferro nelle HSC dei topi talassemici th3, suggerendo un aumento dell'assorbimento e dell'immagazzinamento del ferro. Coerentemente, abbiamo rilevato alti livelli di ferro reattivo libero nel citoplasma e nei mitocondri di th3 HSC, che correlano con alti livelli di ROS. Di conseguenza, i mitocondri sono alterati, con ridotta massa e attività. I progenitori multipotenti th3 hanno ereditato mitocondri disfunzionali poiché la correzione dell'attività mitocondriale si è verificata nella transizione verso progenitori più differenziati. In linea con la disfunzione mitocondriale, le HSC th3 hanno una ridotta produzione di ATP mediante OXPHOS e dipendono dalla glicolisi. La riduzione in vivo dei ROS mitocondriali ha ripristinato l'attività e il metabolismo mitocondriali e ha aumentato la frequenza e la quiescenza delle HSC th3, dimostrando così che lo stress ossidativo è la causa della disfunzione mitocondriale e dei potenziali difetti delle HSC. È importante sottolineare che la somministrazione in vivo di ferro destrano a topi wt ha generato eccesso di ferro intracellulare e stress ossidativo mitocondriale e una ridotta attività mitocondriale nelle HSC, indicando che il sovraccarico di ferro da solo è sufficiente per compromettere i mitocondri. Il nostro studio rivela che il sovraccarico di ferro ha un impatto diretto sul metabolismo delle HSC inducendo stress ossidativo e disfunzione mitocondriale. Le alterazioni dell'attività mitocondriale e del profilo metabolico, in risposta al sovraccarico di ferro, potrebbero alterare la funzione delle HSC. Questa ricerca aggiungerà nuove informazioni sul ruolo del ferro nella regolazione del metabolismo delle HSC e fornirà nuove conoscenze utili per migliorare le condizioni cliniche caratterizzate da sovraccarico di ferro, come BThal.
Sahm, Alexander [Verfasser]. "Prognose der Schnittkräfte bei der HSC-Bearbeitung / Alexander Sahm." Aachen : Shaker, 2003. http://d-nb.info/1174513950/34.
Full textBonkhofer, Florian. "Identification of novel Runx1 targets involved in HSC development." Thesis, University of Oxford, 2017. https://ora.ox.ac.uk/objects/uuid:4badf9f4-f796-4063-8176-dd57644fd811.
Full textHotakainen, L. (Lari). "HSC-3 -solujen eksosomit ja CAV-1 -proteiinin ilmeneminen." University of Oulu, 2017. http://urn.fi/URN:NBN:fi:oulu-201705312281.
Full textEnk, Dirk. "Untersuchungen zum dynamischen Stabilitätsverhalten von Fräswerkzeugen zur HSC-Bearbeitung." Essen Vulkan-Verl, 2009. http://d-nb.info/995794677/04.
Full textSakamaki, Taro. "Hoxb5 defines the heterogeneity of self-renewal capacity in the hematopoietic stem cell compartment." Doctoral thesis, Kyoto University, 2021. http://hdl.handle.net/2433/263564.
Full textGhiaur, Gabriel. "The role of Rho GTPases in hematopoietic stem cell biology RhoA GTPase regulates adult HSC engraftment and Rac1 GTPases is important for embryonic HSC /." Cincinnati, Ohio : University of Cincinnati, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1204374567.
Full textZayas, Jennifer. "Regulation of HSC Self-Renewal and Differentiation by Pumilio Proteins." Scholarly Repository, 2008. http://scholarlyrepository.miami.edu/oa_dissertations/300.
Full textMunirathnam, Madhu. "Einfluss masseoptimierter Kragarmstrukturen auf die dynamische Bahngenauigkeit von HSC-Fräsmaschinen /." Aachen : Shaker, 2008. http://d-nb.info/990312801/04.
Full textStroh, Carsten. "Über die Spline-basierte Werkzeugweggenerierung für die HSC-Schlichtbearbeitung von Freiformflächen /." Aachen : Shaker, 2008. http://d-nb.info/989297888/04.
Full textSöderling, Filip. "Structural analysis of HSC hull design for JFD swimmer delivery system." Thesis, KTH, Marina system, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-234836.
Full textKompositmaterial är vida använt som material för mindre snabbgående fartyg, tack vare dess styrka relativt vikt. James Fisher Defence har tagit fram ett dykartransportsystem kallat SEAL Carrier vars skrov är byggt av kolfiber. Det ursprungliga skrovet är designat utifrån regelver-ket av Det Norske Veritas (DNV) från 2011. Analysen i denna rapport har som mål att identifiera hur väl detta skrov står sig mot en uppdateringen av regelverket från 2011. Ett antal struk-turella delar har visat sig inte uppnå designkraven som ställs i detta regelverk, vilket väckte in-tresse för vad som faktiskt skiljer sig mellan utgåvorna. Sedan sammanslagningen mellan DNV och Germanischer Lloyd (GL) har en harmoniserad gemensam utgåva av regelverket publicerats 2016. För vissa paneler har resultaten visat att regelverken skiljer sig upp mot 500% samt att ett kriterium gällande maximal tillåten stress skiljer sig upp till 42%. Denna analys kommer att behandlas som underlag för en vidareutveckling utav nästa generations SEAL Carrier, vilken kan komma att certifieras av DNV-GL. Författaren föreslår materialtester för att undvika os-äkerheter beskrivna i rapporten, tillsammans med högre detaljnivå i skrovkonstruktion för att minska felmarginalen i analysen.
Dresselhaus, Manfred. "Ein Mustererkennungssystem für bildverarbeitungsgestützte Robotikaufgaben auf der Basis des hierarchischen Strukturcodes HSC /." Düsseldorf : VDI-Verl, 1991. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=002720084&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.
Full textDixon, Wendy P. "Selection procedures relating to Australian vocal repertoire for mid-adolescent HSC performers." University of Sydney, 2007. http://hdl.handle.net/2123/1590.
Full textThis thesis documents an investigation of the selection procedures relating to Australian vocal repertoire for mid-adolescent and Higher School Certificate (New South Wales) performers, as used by private singing teachers, school music teachers and singing students. It explores the similarities and differences in the criteria employed in these selections. Semi-structured interviews were the source of data and were conducted with participants from these three categories as well as two composers. The participants evinced highly disparate views. The private singing teachers believed that repertoire should be dictated by the technical ability and physiological constraints of mid-adolescent students and that their role in selecting repertoire was related to the long term vocal growth of each individual. They felt that the school music teachers vetted their repertoire choices with no useful explanation of their reasoning, while the school music teachers noted that students frequently presented repertoire that was too difficult or that was not readily communicated with the audience. The ability of mid-adolescent singers to communicate with and engage an audience was the prime concern of the school music teachers. The students wanted to impress their examiners and believed that infrequently heard repertoire was the best choice, though this was not endorsed by the teachers. There was a perception that the students would perform at their best when they chose repertoire to which they could relate emotionally. Many private singing teachers and school music teachers are not aware of the very broad range of contemporary Australian music and its divergent characteristics. However, there is a shortage of appropriate Australian repertoire that addresses the physiological and emotional needs of mid-adolescent singers.
Munirathnam, Madhu [Verfasser]. "Einfluss masseoptimierter Kragarmstrukturen auf die dynamische Bahngenauigkeit von HSC-Fräsmaschinen / Madhu Munirathnam." Aachen : Shaker, 2008. http://d-nb.info/1161304029/34.
Full textDixon, Wendy. "Selection procedures relating to Australian vocal repertoire for mid-adolescent HSC performers." Connect to full text, 2006. http://hdl.handle.net/2123/1590.
Full textTitle from title screen (viewed 19 March 2008). Submitted in fulfilment of the requirements for the degree of Master of Music (Music Education) to the Sydney Conservatorium of Music. Includes bibliographical references. Also issued in print format.
McGraw, Kelli. "Innovation and change in the 1999 NSW HSC English syllabus: Challenges and problems." Thesis, The University of Sydney, 2010. http://hdl.handle.net/2123/16459.
Full textVincent, Karen Jane. "Studies on the expression and regulation of transcription factors in hepatic stellate cells." Thesis, University of Southampton, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323790.
Full textStroh, Carsten [Verfasser]. "Über die Spline-basierte Werkzeugweggenerierung für die HSC-Schlichtbearbeitung von Freiformflächen / Carsten Stroh." Aachen : Shaker, 2008. http://d-nb.info/1162792590/34.
Full textMcGraw, Kelli. "Innovation and change in the 1999 NSW HSC English syllabus: Challenges and problems." Thesis, University of Sydney, 2010. https://eprints.qut.edu.au/114957/1/114957.pdf.
Full textZhou, Xiang. "Expressions of hsc/hsp70 cDNAs in bacteria and comparison with tissue-isolated proteins." Thesis, The University of Arizona, 1993. http://hdl.handle.net/10150/144636.
Full textTanaka, Masayuki, Kenneth C. Wong, Anupreeta More, Arsha Dezuka, Eiichi Egami, Masamune Oguri, Sherry H. Suyu, et al. "A SPECTROSCOPICALLY CONFIRMED DOUBLE SOURCE PLANE LENS SYSTEM IN THE HYPER SUPRIME-CAM SUBARU STRATEGIC PROGRAM." IOP PUBLISHING LTD, 2016. http://hdl.handle.net/10150/621400.
Full textHuerga, Encabo Hector 1989. "Analysis of transcription mechanisms that limit type I interferon responses." Doctoral thesis, Universitat Pompeu Fabra, 2018. http://hdl.handle.net/10803/666035.
Full textNicholson, Philippa Ruth. "The roles of Shc, PPZA and Hsc 70 binding in transformation by polyoma middle-T antigen." Thesis, Imperial College London, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.252110.
Full textNafi, Valencia Erika. "The role of hsc-70 in very low density lipoprotein tranport vesicle golgi fusion complex formation." Honors in the Major Thesis, University of Central Florida, 2012. http://digital.library.ucf.edu/cdm/ref/collection/ETH/id/592.
Full textB.S.
Bachelors
Burnett School of Biomedical Sciences
Molecular Biology and Microbiology
MALLARDO, MARIA. "DISSECTING THE ROLE OF THE CYTOPLASMIC MUTANT NUCLEOPHOSMIN IN ACUTE MYELOID LEUKAEMIA DEVELOPMENT." Doctoral thesis, Università degli Studi di Milano, 2014. http://hdl.handle.net/2434/234152.
Full textLendínez, Javier González. "Unravelling a new role of Notch signalling pathway in HSC development using a Hes1-EGFP mouse model." Thesis, University of Edinburgh, 2016. http://hdl.handle.net/1842/25897.
Full textBarria, Emily Alpas. "HSC differentiation is fleeting, but self-renewal is forever : in vitro self-renewal of hematopoietic stem cells /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2004. http://wwwlib.umi.com/cr/ucsd/fullcit?p3123666.
Full textJing, Duohui. "Mobilisation, Isolation and Coculture of Haematopoietic Stem Cells." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2011. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-39915.
Full textWeber, Lauren. "Reading the Curriculum: Empathic Education in Shakespeare, Keats and Haddon." Thesis, The University of Sydney, 2021. https://hdl.handle.net/2123/25983.
Full textStosich, Michael Steven. "MICRO-CT EVALUATION OF MSC/HUVEC/HSC-MEDIATED ANGIOGENESIS IN LARGE PCL-ßTCP CONSTRUCTS IMPLANTED IN NUDE MICE." Master's thesis, Temple University Libraries, 2011. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/213128.
Full textM.S.
Stable vascular networks are prerequisite for scaffold-guided reconstruction of large voluminous bony defects. However, induction of angiogenesis remains a major challenge for bone-tissue engineering. The present in vivo study evaluates human umbilical cord derived vascular endothelial cells (HUVEC) or hematopoietic stem cells(HSCs)-mediated formation of microvascular networks in a biocompatible PCL-ßTCP hybrid scaffold in an immuno-compromised nude mouse. The PCL-ßTCP scaffold (30% PCL: 70% ßTCP; 70% porosity) with interconnected open pores was fabricated in 6x6x6mm cubes. They were seeded with human mesenchymal stem cells (MSCs) plus HUVECs or HSCs. Groups (N=12) consisted of scaffold samples seeded with MSCs alone, MSCs and HUVECs, MSCs and HSCs, and no cells. Cell suspension containing thrombin was evenly seeded into the micro porous constructs, sealed with fibrinogen, and implanted into immunodeficient mice for 8 weeks. At the end of the 8th week, mouse blood vessels were perfused with radiopaque microfil. The scaffolds were harvested, demineralized and analyzed for neovasculature by microcomputed tomography (micro- CT). The formation of microvasculature was significantly higher in the MSC-HUVEC and MSC-HSC constructs than those seeded with MSC alone or cell-free constructs. Volumetric analysis showed that MSC-HUVEC constructs contained 250% more microvasculature than cell-free construct and 180% more microvasculature than MSC or MSC-HSC constructs. Furthermore, neovascularization in the cell free construct was limited to the surface of the construct. The development of neovascularization from noninvasive sources constitutes an important step in the development of clinical strategies for tissue vascularization of large craniofacial defects.
Temple University--Theses
Li, Yang. "Blast Performance of Reiforced Concrete Beams Constructed with High-Strength Concrete and High-Strength Reinforcement." Thesis, Université d'Ottawa / University of Ottawa, 2016. http://hdl.handle.net/10393/35261.
Full textLapenna, Antonio. "T cells development in vitro : a minimalist approach." Thesis, Cranfield University, 2012. http://dspace.lib.cranfield.ac.uk/handle/1826/7797.
Full textLiu, Wei. "Rational targeting of Cdc42 in hematopoietic stem cell mobilization and engraftment." University of Cincinnati / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1303845649.
Full textKettern, Nadja [Verfasser]. "Das Hsc/Hsp70 Co-Chaperon-Netzwerk kontrolliert die Antigenaggregation und -präsentation während der Immunzellreifung / Nadja Kettern. Mathematisch-Naturwissenschaftliche Fakultät." Bonn : Universitäts- und Landesbibliothek Bonn, 2011. http://d-nb.info/1016196814/34.
Full textSirrs, Christopher. "Health and safety in the British regulatory state, 1961-2001 : the HSC, HSE and the management of occupational risk." Thesis, London School of Hygiene and Tropical Medicine (University of London), 2016. http://researchonline.lshtm.ac.uk/2572268/.
Full textSwedosh, Philip, and mikewood@deakin edu au. "An Investigation into the skill levels achieved by mathematics students in the V.C.E. and the H.S.C. mathematics courses." Deakin University, 1994. http://tux.lib.deakin.edu.au./adt-VDU/public/adt-VDU20051017.153947.
Full textRepetylo, Anna H., and n/a. "Factors influencing retention rates in secondary schools within the Wollongong region." University of Canberra. Education, 1993. http://erl.canberra.edu.au./public/adt-AUC20061107.122538.
Full textUlibarri, Roy M. "Habitat Suitability Criteria for Zuni Bluehead Sucker Catostomus discobolus yarrowi and Navajo Nation Genetic Subunit Bluehead Sucker Catostomus discobolus and Comparing Efficiency of AFS Standard Snorkeling Techniques to eDNA Sampling Techniques." Thesis, The University of Arizona, 2016. http://hdl.handle.net/10150/604876.
Full textZebian, Abir. "Etude du facteur de réparation de l’ADN, Xeroderma pigmentosum du groupe C (XPC), dans les cellules souches hématopoïétiques." Thesis, Bordeaux, 2014. http://www.theses.fr/2014BORD0223/document.
Full textDNA damage may accumulate in hematopoietic stem cells (HSC) due to external ormetabolic stresses, leading to perturbation in their function and/or maintenance. Nucleotide excisionrepair (NER), initiated in the DNA by the stop of transcription (TCR) or by the recognition of distortionsin transcribed regions (GGR), is necessary for long-term hematopoiesis. XPC, a key factor in GGR, isimplicated in oxidative stress. The laboratory has demonstrated that XPC loss leads to theaccumulation of mutations, metabolic stress and carcinogenesis. Our objective is to evaluate XPCexpression and its role in HSC maintenance and differentiation. Results showed that XPC is highlyexpressed in immature CD34+ cells compared to mature CD34- cells. In addition, XPC appeared withthree different molecular weights, certainly linked to post-translational modifications. XPC silencing byshRNA did not affect the proliferation or the progenitor ability of CD34+ cells in vitro. However, deficientcells transplanted in immunodeficient mice disappeared progressively, suggesting the loss of HSCs ortheir differentiation capacity. Postulating that mutations accumulate with time, we have studiedhematopoiesis in young and aged XPC deficient mice. Differences described in young and agedhematopoiesis systems were found but, surprisingly, no difference was observed between wild typeand mutant mice at any age or genotoxic stress. Data from human cells demonstrate a potential rolefor XPC in HSC but new investigations are necessary to better understand the mechanisms implicatedand if XPC may participate in leukemogenesis
Plensa, Nebot Isabel. "Hiperplasia suprarrenal congénita por defecto en la enzima 21-hidroxilasa: caracterización por el sistema HLA y aportación de la biología molecular, La." Doctoral thesis, Universitat de Barcelona, 2003. http://hdl.handle.net/10803/2982.
Full textEsta enfermedad presenta un espectro muy amplio de manifestaciones clínicas; desde formas severas que se presentan al nacimiento, en las que la virilización puede ir o no acompañada de un síndrome pierde sal, hasta formas más leves en que los signos de virilización se presentan más tardíamente. La incidencia de las formas clásicas de este déficit, oscila alrededor de 1:10.000-15.000 nacimientos, mientras la de las formas no clásicas se ha estimado en 1:1.000 en la población blanca, con marcadas variaciones étnicas.
El objetivo ha sido la caracterización clínica, biológica y genética de la HSC por déficit en 21-hidroxilasa de una población de la Comunitat Autònoma de Catalunya, con el fin de poder realizar un diagnóstico etiológico concluyente y establecer el consejo genético familiar completo.
Han colaborado 53 familias (106 cromosomas no emparentados) con al menos un hijo diagnosticado de HSC por déficit en 21-hidroxilasa, siendo el total de individuos estudiados de 214. De este total, 71 son pacientes que según los síntomas clínicos fueron clasificados en: 13 SW; 5 SV y 53 NC; 102 son progenitores y 41 son hermanos de dichos pacientes, ninguno de ellos clínicamente afecto.
Para la determinación de la función hormonal se practicó una prueba de estimulación con corticotropina (test de ACTH), con valoración de las concentraciones plasmáticas de 17-hidroxiprogesterona antes y tras 30 minutos de la inyección. La determinación de los antígenos de Clase I y Clase II del Sistema HLA, se realizó mediante la técnica de Microlinfocitotoxicidad. El análisis genético consistió en la detección de grandes reordenamientos mediante la técnica de "Southern blot", y la detección de nueve de las mutaciones puntuales más comunes mediante el diseño de una nueva metodología en la que se emplean dos estrategias según la mutación a detectar: ganancia o pérdida de al menos una diana de restricción en el producto de PCR amplificado a partir de DNA genómico o creación de una diana de restricción por amplificación (ACRS-PCR) y posterior digestión con una enzima de restricción apropiada.
El test de ACTH se confirma como fiable, ya que no se observaron falsos positivos ni falsos negativos en los valores obtenidos de 17-hidroxiprogesterona post estimulación. El nomograma de 17-hidroxiprogesterona proporciona un patrón hormonal que facilita, en la mayoría de los casos, el diagnóstico de las distintas formas de presentación clínica del déficit. No se observó la presencia del antígeno HLA-Bw47 en ninguno de los grupos analizados. Las formas no clásicas se encuentran significativamente asociadas al haplotipo HLA-B14-DR1, mientras que las formas clásicas al antígeno HLA-B5(w51).
La metodología utilizada para el análisis molecular del gen ha permitido detectar la mutación causal en el 89,62% de los cromosomas afectos, evaluados globalmente, consolidándose ésta como altamente efectiva en el diagnóstico de esta patología. Las mutaciones más frecuentes observadas han sido: Intrón 2 (50%) en SW; Intrón 2 (50%) y I172N (16,6%) en SV y V281L (61,5%) en NC. El análisis de los pedigrees ha permitido diagnosticar veintiséis formas crípticas y revelar en dos casos la presencia de mutaciones originadas de novo. La concordancia hallada entre el genotipo y el fenotipo ha sido del 97,2%, de ahí, que las predicciones desde el genotipo han de ser hechas todavía con gran cautela.
Mesquita, Fernanda Cristina de. "Efeito da liraglutida sobre a fibrose hep?tica e c?lulas estreladas ativadas." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2017. http://tede2.pucrs.br/tede2/handle/tede/7593.
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Liver fibrosis is the wound healing response to repeated injury of the liver. This process begins with the damage of the parenchymal cells and subsequent inflammation, characterized by the rupture of the hepatic architecture associated to the increase of the expression of the components of the extracellular matrix. The development of hepatic fibrosis is based on the activation of hepatic stellate cells (HSC) that undergo phenotypic changes and are characterized by loss of vitamin A deposition and increased cell proliferation, triggering hepatic microcirculatory dysfunction and fibrogenesis in patients with chronic liver disease (CLD). Liraglutide is a GLP-1 agonist (glucagon-like peptide 1) well established as an antidiabetic drug, but also has anti-inflammatory properties, in addition to the effectiveness for NAFLD (non-alcoholic fatty liver disease). Therefore, the aim of this study was to evaluate the effects of liraglutide on the HSC phenotype and liver microvascular function using diverse pre-clinical models of CLD. The results obtained demonstrate that Liraglutide de-activated human and rat HSC phenotype through a GLP1-Rindependent mechanism. Liraglutide did not affect the HSC viability but decreased cell proliferation. CLD-rats receiving liraglutide exhibited significantly lower portal pressure (-20%) with a consequent reduction in intrahepatic vascular resistance. There was also a marked improvements in hepatic vascular function, fibrosis, HSC phenotype and sinusoidal endothelial phenotype. The anti-fibrotic effects of liraglutide were confirmed in human liver tissue. In conclusion, this study demonstrates for the first time that liraglutide improves hepatic sinusoidal endothelium in clinically relevant experimental models of cirrhosis, which leads to improvement in fibrosis and portal hypertension, and therefore is valid in the treatment of advanced chronic liver disease.
A fibrose hep?tica ? a resposta cicatricial do f?gado ? les?es repetidas. Este processo inicia com o dano das c?lulas parenquimatosas e consecutiva inflama??o, caracterizado pelo rompimento da arquitetura hep?tica associada ao aumento da express?o dos componentes da matriz extracelular. O desenvolvimento da fibrose hep?tica ? baseado na ativa??o das c?lulas hep?ticas estreladas (HSC) que sofrem mudan?as fenot?picas e se caracterizam pela perda do dep?sito de vitamina A e aumento da prolifera??o celular, desencadeando disfun??o microcirculat?ria hep?tica e fibrog?nese nos pacientes com doen?a hep?tica cr?nica (CLD). A liraglutida ? um an?lago do GLP-1 (glucagon-like peptide 1) bem estabelecido como f?rmaco antidiab?tico, mas que tamb?m possui propriedades antinflamat?rias, al?m da efetividade para NAFLD (doen?a hep?tica gordurosa n?o alco?lica). Por essa raz?o, o objetivo deste estudo foi avaliar os efeitos da liraglutida sobre o fen?tipo das HSC e a fun??o microvascular hep?tica utilizando diversos modelos pr?-cl?nicos de CLD. Os resultados obtidos demonstram que a liraglutida desativou o fen?tipo das HSC humanas e de ratos atrav?s de um mecanismo independente do receptor GLP1. A liraglutida n?o afetou a viabilidade das HSC mas diminuiu a prolifera??o celular. Os ratos com CLD que receberam liraglutida apresentaram press?o portal significativamente menor (-20%) com consequente redu??o da resist?ncia vascular intra-hep?tica. Houve tamb?m uma acentuada melhoria na fun??o vascular hep?tica, fibrose, fen?tipo das HSC e fen?tipo endotelial sinusoidal. Os efeitos anti-fibr?ticos da liraglutida tamb?m foram confirmados em tecido hep?tico humano. Como conclus?o, este estudo demonstra pela primeira vez que a liraglutida melhora o endotelio sinusoidal hep?tico em modelos experimentais clinicamente relevantes de cirrose, o que leva a melhora no quadro fibr?tico e na hipertens?o portal e, portanto, pode ser v?lido no tratamento da doen?a hep?tica cr?nica avan?ada.
Jayavaradhan, Rajeswari. "Optimization of Gene Editing Approaches for Human Hematopoietic Stem Cells." University of Cincinnati / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1543919940219677.
Full textCardoso, Alexandre da Silva Paes. "Predição do comportamento dinâmico de sistemas compostospor eixo-árvore, sistema de fixação e ferramenta de corte de máquinas ferramenta para altas velocidades (HSC)." reponame:Repositório Institucional da UFSC, 2006. https://repositorio.ufsc.br/xmlui/handle/123456789/158353.
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A predição de condições estáveis de corte é um dos requisitos críticos para a operacionalização da usinagem em altas velocidades. O presente trabalho buscou estudar o comportamento dinâmico de sistemas compostos por eixo-árvore, sistema de fixação e ferramenta de corte de máquinas de usinagem em altas velocidades (HSC), através de medições experimentais e simulações de vibrações por Elementos Finitos, identificando-se os parâmetros modais dos conjuntos, em busca de um maior entendimento dos fenômenos associados à dinâmica do fresamento em altas velocidades. Foram geradas também as cartas de estabilidade dos conjuntos estudados, através de métodos analíticos disponíveis na literatura, para servirem de referência na otimização dos processos de usinagem em altas velocidades.
Abstract : The prediction of steady conditions of cut is one of the critical requirements for the practical implementation of high-speed machining. The present work searched to study the dynamic behavior of systems composites for axle-tree, system of setting and tool of cut of machines of milling in high speed (HSC), through experimental measurements and simulation of vibrations for Finite Elements, identifying the modal parameters of the sets, in search of a bigger agreement of the phenomena associates to the dynamics of milling in high speed. The letters of stability of the studied sets had also been generated, through available analytical methods in literature, to serve of reference in the practical implementation of high-speed machining.
Souza, Adriano Fagali de. "Contribuições ao fresamento de geometrias complexas aplicando a tecnologia de usinagem com altas velocidades." Universidade de São Paulo, 2004. http://www.teses.usp.br/teses/disponiveis/18/18135/tde-14012005-101635/.
Full textIn recent years the number of products containing free-form shapes produced by dies and molds have been growing noticeably. However, the milling process used to manufacture those dies and molds does not meet their requirements. The arrival of the CAD/CAM systems and the High Speed Cutting Technology (HSC) helps to improve this manufacturing process. Although to obtain the surface quality needed to meet the dies and molds requirements, a hand finishing still requires. It involves time, money and decrease the product quality. Technological limitations in the CAD/CAM/CNC chain limit the feed rate when milling free-form shape. It also has a negative effect on the surface roughness. Besides, this kind of milling still lacks scientific knowledge of the cutting process. With the aim to support the dies and moulds fabrication, this work presents an overview about HSC Technology; the cutting forces in a non-planar milling; the cutting tool deflection; a detailed description of the process chain involving the CAD/CAM/CNC systems and the methodologies used by those systems to generate and accomplish free-free tool-paths. Free-form milling experiments applying the HSC Technology were made to study the behavior of this process, and the outcomes are presented
Isaksson, Adam. "Evaluation of Scale-up Model for Flotation with Kristineberg Ore." Thesis, Luleå tekniska universitet, Institutionen för samhällsbyggnad och naturresurser, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-70117.
Full textSyftet med det här examensarbetet var att utföra en detaljprovtagning av flotationskretsen i Bolidens anrikningsverk, massbalansera data och sedan utvärdera en modell för uppskalning av resultat från laboratorieflotationer. Modellen antar att hälften av utbytet till returgodset i ett satsvis laboratorieförsök skulle rapportera till det slutliga koncentratet om det återcirkulerades, såsom i ett anrikningsverk. Den har använts av Boliden Mineral AB sedan 1982 men utvärderades senast 1986. Kategoriskt kan den ses som en uppskalningsmodell av typen öppen krets med skalfaktorer. Projektet baserades på en komplex Ag-Au-Cu-Pb-Zn sulfidmalm från gruvan i Kristineberg. Laboratorieförsök utfördes för att ta fram koncentrat av CuPb, Cu, Pb och Zn, med pulpprover från driften som utgångsmaterial. Programmet HSC 9.3 användes för att massbalansera datan från provtagningen. Det bestämdes att modellen skulle anses som godtagbar ifall den kunde förutspå driftresultatet med samma noggrannhet som 1986. Ett simulerat försök av typen sluten krets (Agar & Kipkie, 1978) identifierades som den mest intressanta alternativmodellen och även den utvärderades. Resultaten visade att modellen än idag ger godtagbara förutsägelser med samma noggrannhet som 1986. Modellen var särskilt bra på att förutspå halt och utbyte av den huvudsakliga metallen till dess eget koncentrat. Den förutspådde exempelvis en 18 % högre halt och 11 % lägre utbyte av Cu till CuPb-koncentratet, samt 3 % lägre halt och 11 % lägre utbyte av Zn till Zn-koncentratet. Den alternativa modellen gav sämre förutsägelser med avseende på halter, men bättre med avseende på utbyten. Den var bättre på att förutspå beteendet hos låghaltiga föroreningar såsom As och Bi. Rekommendationen är att kombinera de två modellerna till en "blandkretsmodell". I den här undersökningen hade ett sådant alternativ förutspått en 18 % högre halt och 7 % lägre utbyte av Cu till CuPb-koncentratet, samt 3 % lägre halt och 1 % högre utbyte av Zn till Zn-koncentratet jämfört med driftresultatet. En sådan modell tycks ge bättre förutsägelser, men bör testas på fler prover och malmtyper. Den borde åtminstone kunna användas för att kontrollera trovärdigheten hos resultaten förutspådda av den nuvarande modellen.
Fosu, Allen Yushark. "Development of a Chloride Route for Lithium Extraction from Spodumene." Electronic Thesis or Diss., Université de Lorraine, 2023. http://www.theses.fr/2023LORR0094.
Full textLithium is a major component of Li-ion batteries, used in the manufacture of many portable electronic devices. The energy transition is driving the shift from thermal to electric and hybrid vehicles, which relies mainly on the use of Li-ion batteries for reversible energy storage. The development of electric vehicles based on lithium-ion technology is responsible for a record demand for lithium salt (mainly lithium carbonate and hydroxide). Spodumene is the main source of lithium from ores. Its processing requires a phase transformation from α-form to β-form, followed by roasting leading to the formation of a lithium salt after a leaching, purification, and recovery steps. In this thesis, spodumene concentrate from the Pilbara region of Western Australia was characterized for thermal and hydrometallurgical processing. Heat treatment is responsible for the formation of cracks in the grains which become more noticeable with increasing temperature. Disintegration of the material, melting and agglomeration with minerals contained in the gangue have also been observed by increasing the temperature up to 1050 °C. Apparent activation energies of 655±20 kJ mol-1 was calculated for the transformation of α-spodumene which confirms a strong temperature dependence for polymorphic transformations of spodumene. Subsequently, we investigated an alternative route to conventional methods (sulphuric acid process) to treat the spodumene concentrate with the aim of reducing the high energy consumption of the phase transformation and sulphate roasting steps. This was achieved by direct chlorination of α-spodumene with calcium chloride, followed by water leaching of the residue to recover lithium chloride. Analysis of the residue obtained after leaching indicated that the α-form was the only polymorph present, suggesting that extraction occurs directly from the α-phase. Under optimal conditions, heat treatment at 1000 °C for 60 minutes of the spodumene concentrate in the presence of calcium chloride at a calcium chloride/spodumene molar ratio of 2.0 is required to extract nearly 90% of lithium and recover 85% in the leach liquor. An apparent activation energy of about 122±6 kJ mol-1 was calculated for temperatures ranging from 800 to 950 ℃. The liquor obtained after leaching was purified by ion exchange and solvent extraction to recover lithium chloride of sufficient purity for consideration as a precursor in the production of lithium-ion battery materials