Relevant bibliographies by topics / HPLC (high-performance liquid chromatography)

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'HPLC (high-performance liquid chromatography)'

Author: Grafiati
Published: 4 June 2021
Last updated: 9 February 2022

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Journal articles on the topic "HPLC (high-performance liquid chromatography)"

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Lochman, J., O. Šerý, L. Jankovský, and V. Mikes. "Discrimination of Czech Armillaria species based on PCR method and high performance liquid chromatography." Plant Protection Science 38, SI 1 - 6th Conf EFPP 2002 (January 1, 2002): S31—S34. http://dx.doi.org/10.17221/10316-pps.

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The genus Armillaria belongs to basidiomycetes and has been known to induce root rot disease and to cause extensive economic losses to a forest crop. We analysed about 40 isolates of Armillaria collected in Czech Republic by PCR and restriction analysis using gel electrophoresis and ion-exchange HPLC. Restrictase Hinf I was able to discriminate all investigated Armillaria species. The sensitivity and resolution of HPLC method was better than that performed by gel electrophoresis. HPLC was able to detect some heterozygous. The results prove the similarity of the species A. borealis, A. cepistipes, A. gallica, A. ostoyae in difference of A. mellea and A. tabescens.
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Regnier, Fred E. "High-performance liquid chromatography (HPLC) of proteins." Fresenius' Zeitschrift für analytische Chemie 333, no. 7 (January 1989): 728. http://dx.doi.org/10.1007/bf00476585.

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Adel, E. Ibrahim, Elhenawee Magda, Saleh Hanaa, and M. Sebaiy Mahmoud. "Overview on liquid chromatography and its greener chemistry application." Annals of Advances in Chemistry 5, no. 1 (April 7, 2021): 004–12. http://dx.doi.org/10.29328/journal.aac.1001023.

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This literature review is concerning with liquid chromatography specifically high performance liquid chromatography (HPLC), Ultra high performance liquid chromatography (UHPLC), chromatography theory, chromatographic parameters, monolithic columns, principles of green chemistry and its application ingreen chromatography.
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MORIOKA, Masaaki, Yozo OHASHI, Yasukazu SEN, Fumito KOMATSU, Yuko KONISHI, and Yukitoshi FUJITA. "Analysis of Corticoids in Adrenal Glands by High Performance Liquid Chromatography (HPLC)." Folia Endocrinologica Japonica 69, no. 1 (1993): 55–66. http://dx.doi.org/10.1507/endocrine1927.69.1_55.

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An, Soonmo, Jiyoung Lee, and Wayne S. Gardner. "Stable Isotope Measurement of Ammonium Using HPLC-RTS (high performance liquid chromatography-retention time shift)." Sea 18, no. 1 (February 28, 2013): 47–52. http://dx.doi.org/10.7850/jkso.2013.18.1.47.

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Akram, N. MD. "A Review on High Performance Liquid Chromatography (HPLC)." International Journal for Research in Applied Science and Engineering Technology 6, no. 2 (February 28, 2018): 488–92. http://dx.doi.org/10.22214/ijraset.2018.2098.

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Zhang, Xingping, Jiujun Wang, Qinghua Wu, Li Li, Yun Wang, and Hualin Yang. "Determination of Kanamycin by High Performance Liquid Chromatography." Molecules 24, no. 10 (May 17, 2019): 1902. http://dx.doi.org/10.3390/molecules24101902.

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Kanamycin is an aminoglycoside antibiotic widely used in treating animal diseases caused by Gram-negative and Gram-positive infections. Kanamycin has a relatively narrow therapeutic index, and can accumulate in the human body through the food chain. The abuse of kanamycin can have serious side-effects. Therefore, it was necessary to develop a sensitive and selective analysis method to detect kanamycin residue in food to ensure public health. There are many analytical methods to determine kanamycin concentration, among which high performance liquid chromatography (HPLC) is a common and practical tool. This paper presents a review of the application of HPLC analysis of kanamycin in different sample matrices. The different detectors coupled with HPLC, including Ultraviolet (UV)/Fluorescence, Evaporative Light Scattering Detector (ELSD)/Pulsed Electrochemical Detection (PED), and Mass Spectrometry, are discussed. Meanwhile, the strengths and weaknesses of each method are compared. The pre-treatment methods of food samples, including protein precipitation, liquid-liquid extraction (LLE), and solid-phase extraction (SPE) are also summarized in this paper.
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Hasany, Syeda Mariam, Rahila Huma, Sumia Akram, Rizwan Ashraf, and Muhammad Mushtaq. "Maceration-Mediated Liquid–Liquid Extraction and Reverse-Phase High-Performance Liquid Chromatography-Based Pragmatic Analysis of Silybins." Journal of Chromatographic Science 58, no. 8 (July 24, 2020): 779–87. http://dx.doi.org/10.1093/chromsci/bmaa035.

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Abstract This study presents a pragmatic and easily scalable maceration-mediated liquid–liquid extraction (MMLLE) and reverse-phase high-performance liquid chromatography (RP-HPLC)-based determination of Silybins from plant material (Curcuma longa L.). The processing of calibration standards revealed that the RP-HPLC method was linear over a concentration range of 1–100 μg/mL with regression coefficient (R2) > 0.9950, limit of detection 0.02 μg/mL and limit of quantification <0.07 μg/mL. The optimum chromatographic conditions resolved Silybin A, Silybin B, Isosilybin A and Isosilybin B within 5 min of analysis time. The reproducible recovery rates of spiked flavonolignans (96.24–115.40%) from quality controls established the effectiveness of MMLLE procedure prior to HPLC determination. The real-time analysis revealed the presence of silybins in C. longa roots. The results further endorse that MMLLE prior to chromatographic determination may provide a more pragmatic analytical solution for the analysis/isolation of silybins.
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K., Hari Ramakrishnan, and Janaky Ranjithkumar. "Quantitative Determination of Vitamin E Isomers using Gas Chromatography, High Performance Liquid Chromatography and High Performance Thin Layer Chromatography." Indian Journal of Nutrition and Dietetics 54, no. 3 (July 4, 2017): 294. http://dx.doi.org/10.21048/ijnd.2017.54.3.15589.

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Vitamin E, the fat soluble vitamin is present naturally in some foods and added in food supplements, nutraceuticals etc due to its vital biological function as an antioxidant. Various methods are available for the analysis of vitamin E. Especially High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC) are exclusively used for the quantitative evaluation of vitamin E, which has also identified the four different isomeric forms of this vitamin. The rate of losses of this vitamin during food processing and analysis, in addition to their transient dynamics, presents complexities in developing a highly sensitive procedure for their separations. Though effective, HPLC instrument is expensive and comparatively cumbersome. In this prospective, the study was to evaluate the usefulness of High Performance Thin Layer Chromatography (HPTLC) in the analysis of vitamin E. There are methods available using Thin Layer Chromatography for its analysis, but they are not sensitive enough to identify the isomeric forms of vitamin E. In this HPTLC method, the different isomeric forms of vitamin E - α, β, γ and δ were identified. This technique shall be considered as an alternative to the other methods such as HPLC and GC.
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Hamza, Ouakouak, Ben Mohamed Moktar, Ben Chohra Mostafa, and Abdelhamid Zeghdaoui. "Phenobarbital Analysis in Biological Matrix (Blood) by High Performance Liquid Chromatography (HPLC)." International Letters of Chemistry, Physics and Astronomy 20 (October 2013): 31–40. http://dx.doi.org/10.18052/www.scipress.com/ilcpa.20.31.

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In this work, we have tried to contribute to the analysis of phenobarbital in the blood. To do this, we used different analytical techniques such as liquid chromatography (HPLC). The results obtained in the course of our work, we can conclude that: The HPLC method was separate phenobarbital endogenous blood components, and optimizing the conditions of chromatographic separation as the composition of the mobile phase consisting of 20% acetonitrile + 20% methanol + 60% ammonium acetate buffer. The extraction with diethyl ether to pH = 4; The chromatographic column, microbondapack ZORBAX C18. A system of diode-array UV detection at 254 nm.
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Dissertations / Theses on the topic "HPLC (high-performance liquid chromatography)"

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Benson, Andrew James. "High-performance liquid chromatography (HPLC) and high-performance liquid chromatography mass spectrometry (HPLC/MS) for the analysis of date rape drugs." FIU Digital Commons, 2002. http://digitalcommons.fiu.edu/etd/1602.

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The drugs studied in this work have been reportedly used to commit drug-facilitated sexual assault (DFSA), commonly known as "date rape". Detection of the drugs was performed using high-performance liquid chromatography with ultraviolet detection (HPLC/UV) and identified with high performance-liquid chromatography mass spectrometry (HPLC/MS) using selected ion monitoring (SIM). The objective of this study was to develop a single HPLC method for the simultaneous detection, identification and quantitation of these drugs. The following drugs were simultaneously analyzed: Gamma-hydroxybutyrate (GHB), scopolamine, lysergic acid diethylamide, ketamine, flunitrazepam, and diphenhydramine. The results showed increased sensitivity with electrospray (ES) ionization versus atmospheric pressure chemical ionization (APCI) using HPLC/MS. HPLC/ES/MS was approximately six times more sensitive than HPLC/APCI/MS and about fifty times more sensitive than HPLC/UV. A limit of detection (LOD) of 100 ppb was achieved for drug analysis using this method. The average linear regression coefficient of correlation squared (r2) was 0.933 for HPLC/UV and 0.998 for HPLC/ES/MS. The detection limits achieved by this method allowed for the detection of drug dosages used in beverage tampering. This method can be used to screen beverages suspected of drug tampering. The results of this study demonstrated that solid phase microextraction (SPME) did not improve sensitivity as an extraction technique when compared to direct injections of the drug standards.
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Edwardson, P. A. D. "High Performance Liquid Chromatography of polynucleotides and proteins." Thesis, London School of Hygiene and Tropical Medicine (University of London), 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376534.

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Forssén, Patrik. "Adsorption isotherm parameter estimation in nonlinear liquid chromatography /." Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5971.

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DONALD, GREGORY THOMAS. "Model Chiral Ionic Liquids for High Performance Liquid Chromatography Stationary Phases." University of Cincinnati / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1214325450.

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Ritchie, Harald John. "Development of new packing materials for high performance liquid chromatography." Thesis, University of Edinburgh, 1987. http://hdl.handle.net/1842/14283.

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Scholtzova, Angela. "Scale up and modelling of HPLC." Thesis, University College London (University of London), 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368109.

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Walker, Roderick Bryan. "HPLC analysis and pharmacokinetics of cyclizine." Thesis, Rhodes University, 1995. http://hdl.handle.net/10962/d1003279.

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The investigations detailed in this dissertation have been conducted to address the paucity of pharmacokinetic information, in published literature, pertaining to cyclizine. The areas of investigation have included the selective quantitation of both cyclizine and its demethylated metabolite, norcyclizine in serum and urine, assessment of stability of both compounds in stored biological samples, dosage form analysis, dissolution rate testing of tablets, and bioavailability and pharmacokinetics following administration of an intravenous solution, and tablets to humans. High-performance liquid chromatography (HPLC) was used as the main analytical technique throughout these studies. An original HPLC method employing ultraviolet detection with a limit of quantitation of 5μg/ℓ was developed for the determination of cyclizine in serum and both cyclizine and norcyclizine in urine, Solid-phase extraction using extraction columns packed with reversed-phase C18 material, and followed by a simple phase-separation step proved successful for the accurate and precise isolation of the compounds. The validated method was applied to the analysis of serum and urine samples from a pilot study in which a single volunteer was administered 50mg of cyclizine hydrochloride. Several samples collected during the pilot study revealed the presence of both drug and metabolite in concentrations below the limit of detection. In order to improve the selectivity and sensitivity of the analytical method an HPLC method with electrochemical detection operating in the "oxidative-screen" mode was developed. The solid-phase extraction procedure was modified slightly and the method found to be precise, accurate, selective and highly sensitive with a limit of quantitation of Iμg/g/l for both cyclizine and norcyclizine in both serum and urine. This method was applied to the determination of both compounds after intravenous and oral administration of cyclizine to humans. HPLC with electrochemical detection was used for the analysis of samples collected during dissolution studies on the batch of tablets used for pharmacokinetic studies. In addition, this method was used to assess content uniformity of the tablets and of samples from the batch of intravenous ampoules of cyclizine lactate. Dissolution studies showed that all tablets tested passed the compendial specifications for cyclizine. Content uniformity assessment revealed that within-batch uniformity existed for both the tablets and ampoules and, therefore, variations in pharmacokinetic parameters for the drug would more than likely be as a result of inter- and intra-individual variability within the subject population. Pharmacokinetic information for cyclizine was obtained following administration of an intravenous bolus dose of cyclizine lactate as a solution, oral administration of cyclizine hydrochloride as a single dose of 50mg and as fixed multiple doses of 50mg every 8 hours for five days. Further information was acquired following administration of single doses of 100mg and 150mg cyclizine hydrochloride. Data collected from these studies were evaluated using both compartmental and non-compartmental techniques. Cyclizine was rapidly absorbed following oral administration with mean kₐ = 1.54 hr⁻¹ and was found to have an absolute bioavailability (F) of 0.47. The presence of norcyclizine in serum following oral and not intravenous dosing suggests cyclizine is susceptible to "first-pass" metabolism in either the gut wall or the I iver. Mean ClTOT determined following the intravenous dose was 0.865 ℓ/hr/kg. The mean ClTOT of 0.823 ℓ/hr/kg calculated following oral dosing, using a unique value of F for each subject compared favourahly with that obtained following intravenous dosing. Renal clearance of cyclizine is negligihle indicating that non-renal routes of elimination account for the majority of removal of cyclizine form the body. Cyclizine is extensively distributed and the mean Vz following an intravenous dose was 16.70 ℓ/kg. This value is lower than that calculated from all oral studies from which the mean Vz was determined to be 25.74 ℓ/kg. Cyclizine is eliminated slowly with a mean elimination t½ = 20.11 hours. Cyclizine dose not appear to follow dosedependent kinetics and therefore, inability to predict steady state levels are more than likely due to accumulation as a result of frequent dosing rather than saturation of elimination mechanisms. Modelling of intravenous data to one-compartment (lBCM), two-compartment (2BCM) and threecompartment models indicated that the pharmacokinetics of cyclizine can be adequately described by a 3BCM. The drug is rapidly distributed into a "shallow" peripheral compartment (α = 9.44 hr⁻¹ , and k₂₁ = 2.09 hr⁻¹ ), and slowly distributed to the "deep" peripheral compartment (β = 0.451 hr⁻¹ and k₃₁ = 0.120 hr⁻¹ ). Modelling of all oral data indicated that a 2BCM best described the pharmacokinetics of the drug, however, distribution to the peripheral compartment is not as rapid as to the "shallow" peripheral compartment following the intravenous dose. Mean distribution parameters were α = 0.64 hr⁻¹1 and, k₂₁ = 0.39 hr⁻¹. Mean CITOT following intravenous dosing of 0.70 ℓ/hr/kg was similar to the mean CIToT of 0.73 ℓ/hr/kg determined after oral dosing. The mean distribution volume at steady state determined following intravenous dosing (17.78 ℓ/kg) was lower than that obtained from the oral studies (25.52 ℓ/kg). The mean terminal elimination half-lives calculated for cyclizine following fitting of intravenous and oral data was 25.09 hours. In general, mean pharmacokinetic parameters calculated following titting of data to a 2BCM after oral administration correlate closely with those calculated using non-compartmental techniques. However, the pharmacokinetics following intravenous dosing are better described by a 3BCM and a close correlation between parameters estimated using noncompartmental techniques and compartmental techniques is evident when a 3BCM model is used.
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Narine, Raymond. "A novel microcomputer-controlled transport detector for high-performance liquid chromatography." Thesis, King's College London (University of London), 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.281718.

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Lowe, Christian T. "Retention characteristics of water-soluable calixarene modified mobile phases in HPLC /." Connect to the full text of the document, 1998. http://www.ohiolink.edu/etd/view.cgi?ysu997550028.

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Li, F. "Studies of haem biosynthesis and metabolism by high-performance liquid chromatography." Thesis, University of Cambridge, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.378943.

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Books on the topic "HPLC (high-performance liquid chromatography)"

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McMaster, Marvin. HPLC. New York: John Wiley & Sons, Ltd., 2006.

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HPLC, a practical user's guide. New York, N.Y: VCH, 1994.

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Practical HPLC methodology and applications. New York: Wiley, 1992.

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1925-, Kirkland J. J., and Glajch Joseph L, eds. Practical HPLC method development. 2nd ed. New York: Wiley, 1997.

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L, Glajch Joseph, and Kirkland J. J. 1925-, eds. Practical HPLC method development. New York: J. Wiley, 1988.

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Troubleshooting HPLC systems: A bench manual. New York: Wiley, 2000.

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Ahuja, Satinder. Selectivity and detectability optimizations in HPLC. New York: Wiley, 1989.

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Szepesi, Gábor. How to use reverse-phase HPLC. New York, N.Y: VCH Publishers, 1992.

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Royal Society of Chemistry (Great Britain)., ed. HPLC: A practical guide. Cambridge: Royal Society of Chemistry, 1999.

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Meyer, Veronika. Pitfalls and errors of HPLC in pictures. Heidelberg: Huthig, 1997.

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Book chapters on the topic "HPLC (high-performance liquid chromatography)"

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Braithwaite, A., and F. J. Smith. "High Performance Liquid Chromatography (HPLC)." In Chromatographic Methods, 212–90. Dordrecht: Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-009-4093-2_6.

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Perrett, D., and P. White. "HPLC in biomedical and forensic analysis." In High Performance Liquid Chromatography, 205–33. Dordrecht: Springer Netherlands, 1996. http://dx.doi.org/10.1007/978-94-011-0597-2_10.

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Lewis, N. G. "High Performance Liquid Chromatography (HPLC)." In Methods in Lignin Chemistry, 549–67. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-74065-7_39.

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Glöckner, Gottfried. "Detection in Gradient High-Performance Liquid Chromatography." In Gradient HPLC of Copolymers and Chromatographic Cross-Fractionation, 87–98. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-75799-0_7.

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Hansen, Steen Honoré, and Leon Reubsaet. "High-Performance Liquid Chromatography (HPLC) and High-Performance Liquid Chromatography-Mass Spectrometry (LC-MS)." In Bioanalysis of Pharmaceuticals, 123–72. Chichester, UK: John Wiley & Sons, Ltd, 2015. http://dx.doi.org/10.1002/9781118716830.ch7.

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Corbier, Marie, Delphine Schrag, and Sylvain Raimondi. "Ion Exchange-High-Performance Liquid Chromatography (IEX-HPLC)." In Methods in Molecular Biology, 501–6. Totowa, NJ: Humana Press, 2014. http://dx.doi.org/10.1007/978-1-62703-992-5_30.

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Schrag, Delphine, Marie Corbier, and Sylvain Raimondi. "Size Exclusion-High-Performance Liquid Chromatography (SEC-HPLC)." In Methods in Molecular Biology, 507–12. Totowa, NJ: Humana Press, 2014. http://dx.doi.org/10.1007/978-1-62703-992-5_31.

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Tiburcio, A. F., and A. W. Galston. "Analysis of Alkaloids in Tobacco Callus by HPLC." In High Performance Liquid Chromatography in Plant Sciences, 228–42. Berlin, Heidelberg: Springer Berlin Heidelberg, 1987. http://dx.doi.org/10.1007/978-3-642-82951-2_13.

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Chen, Chong-Maw. "Characterization of Cytokinins and Related Compounds by HPLC." In High Performance Liquid Chromatography in Plant Sciences, 23–38. Berlin, Heidelberg: Springer Berlin Heidelberg, 1987. http://dx.doi.org/10.1007/978-3-642-82951-2_2.

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Sütfeld, R. "HPLC of Thiophenes for Phytochemical and Biochemical Research." In High Performance Liquid Chromatography in Plant Sciences, 104–13. Berlin, Heidelberg: Springer Berlin Heidelberg, 1987. http://dx.doi.org/10.1007/978-3-642-82951-2_7.

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Conference papers on the topic "HPLC (high-performance liquid chromatography)"

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Moriyoshi, Akihiro, Shin'Ei Takano, Makoto Ono, Masa'Aki Ogasawara, Masayoshi Tabata, Noboru Miyamoto, and Sachio Ohta. "Analysis of Contribution to SPM by Organic Matters Using High-Performance Liquid Chromatography (HPLC)." In SAE 2002 World Congress & Exhibition. 400 Commonwealth Drive, Warrendale, PA, United States: SAE International, 2002. http://dx.doi.org/10.4271/2002-01-0653.

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Metcalf, Adam, Lucy Hardaker, and Ross Hatley. "Quantitation method for colistimethate sodium in pharmaceutical aerosol samples using high performance liquid chromatography (HPLC)." In ERS International Congress 2016 abstracts. European Respiratory Society, 2016. http://dx.doi.org/10.1183/13993003.congress-2016.pa1071.

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Müller, E., and A. Henschen. "HUMAN PLASMA FIBRINOGEN MOLECULAR WEIGHT VARIANTS:CHARACTERIZATION BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND IDENTIFICATION BY SEQUENCE ANALYSIS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643328.

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Human plasma has been shown to contain several fibrinogen variants which differ from each other in molecular weight. The three most common forms, with molecular weights of 340 kD, 305 kD and 270 kD, have been reported to change considerably in their relative amounts during certain pathophysiological processes such as acute phase reactions, disseminated intravascular coagulation, fibrinolytic disorders, liver disease and cancer. Though it has been suggested that the differences in molecular weight are due to degradation of one or both, respectively, of the carboxy-terminal parts of the Aα-chains, the removed or altered segments have so far never been precisely determined. Consequently, it has only been possible to speculate about the origin of the variants.The aim of this study was to isolate the various molecular weight variants from plasma and to characterize their peptide chain components proteinchemically. Fibrinogen was first isolated from plasma by glycin precipitation and the variants were then separated by stepwise ammonium sulfate precipitation, taking advantage of their different solubility.The peptide chain components of the various fractions were isolated by reversed-phase high-performance liquid chromatography (HPLC). The N-termini were identified by direct sequence analysis. Chemical and enzymatic cleavages of the peptide chains resulted in fragment mixtures which were compared with the corresponding mixtures obtained from commercial fibrinogen by HPLC fingerprinting. Finally, the fragments which differed were identified by N-terminal sequence and amino acid analysis so that the exact C-termini of the peptide chains,especially in the lower molecular weight variants,could be determined. With the help of this information conclusions may also be drawn about the origin of the lower molecular weight variants and about the mechanism by which they may he formed.
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de Vries, J. X., R. Raedsch, A. Stiehl, U. Voelker, I. Walter-Sack, and E. Weber. "EVIDENCE FOR BIIIARY EXCRETION OF PHENPROCOUMON AND ITS METABOLITES IN HUMANS; IDENTIFICATION BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643272.

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Recently it has been shown that in man the oral couma-rin anticoagulant phenprocoumon is eliminated up to 60-70 % in urine and 30-40 % in faeces; in urine phenprocoumon (PH) and its metabolites 7-hydroxy-(7-OH),6-hydroxy-(6-OH) and 4'-hydroxy-(4'-OH) phenprocoumon are present mainly as conjugates. No data so far were available on the biliary excretion of these compounds.We examined bile obtained from four in-patients during PH treatment; bile samples were aspirated in the duodenum at the papilla during routine diagnostic endoscopy and immediately deep frozen before analysis. Samples were extracted both untreated as well as after hydrolysis with 6-glucuronidase/aryl sulfatase and separated by reversed phase gradient elution high performance liquid chromatography (HPLC) with fluorescence detection; for confirmation, the same extracts were methylated and analysed by gas chromatography-mass spectrometry (CG-MS) (J.X.de Vries et al J Chromatogr., 338 (1985) 325). PH, 7-OH, 6-OH and 4'-OH were identified by comparison with synthetic authentic samples'''''''
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Kiso, U., H. Kaudewitz, and A. Henschen. "QUANTIFICATION OF FACTOR XIIIa-MEDIATED FIBRIN CROSSLINKING USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-BASED IDENTIFICATION OF CROSSLINKED γ-CHAINS AND γ-CHAIN COMPONENTS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643309.

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Factor XIIIa catalysis the formation of isopeptide bonds in fibrin vhereby first and quickly the C-termini of two γ-chains in adjacent molecules are crosslinked and then much more slowly several a-chains. The crosslinking sites of the γ-chain are well-known, but those of the a-chain still only tentatively and partially identified. The crosslinking reaction has previously usually been monitored by sodium dodecylsulfat-polyacrylamide gelelectrophoresis (SDS-PAGE) of the mercaptolysed fibrin or fibrin degradation products. More recently, specific antibodies against the crosslinked γ-chain region have been produced which allow immunological assays for crosslinking products, i.e. for D-dimer.The present study deals with novel, high-performance liquid chromatography (HPLC)-based procedures for the identification and quantification of crosslinked γ-chains or γ-chain products. The degree of crosslinking was determined by quantifying the dimer either of the total γ-chain or of its C-terminai cyanogen bromide fragment. For this propose factor Xllla-containing fibrinogen was incubated with thrombin in the presence of calcium ions and cysteine. The reaction was interrupted by the addition of a urea-mercaptoethanol solution after various periods of time and the samples analysed in parallel by reversed-phase HPLC and SDS-PAGE. In both systems the steady decrease first in γ- and then in a-chain and simultaneous increase in γ-chain dimer was observed. The dimeric γ-chain appeared as a well separated and defined peak on HPLC. In the alternative approach crosslinked fibrin, fibrin degradation products or γ-chain were first cleaved by cyanogen bromide and then the resulting fragments were analysed by reversed-phase HPLC. Also here a characteristic component appeared which was identified by sequence analysis as the dimeric C-terminal fragment of the γ-chain and which only was present in crosslinked material.
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Singh, Sameer Kumar, Remila L. Martis, Sujatha, Rani A. Bhat, Pralhad Kushtagi, Lavanya Rai, V. B. Kartha, and C. Santhosh. "Protein profile study of clinical samples of ovarian cancer using high-performance liquid chromatography-laser induced fluorescence (HPLC-LIF)." In Biomedical Optics 2006, edited by Jörg Enderlein and Zygmunt K. Gryczynski. SPIE, 2006. http://dx.doi.org/10.1117/12.647321.

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Müller, E., A. Henschen, and G. Wunderer. "IDENTIFICATION OF A NEW HUMAN KININ, ILE-SER-BRADYKININ, BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND SEQUENCE ANALYSIS IN OVARIAN CARCINOMA ASCITES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642848.

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Human blood has been shown to contain two different kinin precursors, i.e0 the high and the low molecular mass kininogen0 These two kininogens release the same kinins, with the starting sequences Met-Lys-Arg-Pro-, Lys-Arg-Pro- or just Arg-Pro-depending on the releasing enzyme. The kinin starting with Arg-Pro- is denoted as bradykinin. In rats a different kininogen, called T-kininogen, is also present, especially as the major acute-phase protein in this species. The corresponding kinin, T-kinin, has the starting sequence Ile-Ser-Arg-Pro-. This type of kininogen or kinin has previously never been detected in human tissues. However, during the course of the present study evidence for existance of a third human kininogen, giving rise to human T-kinin, was obtained.Ascites from patients with metastatic ovarian carcinoma has been shown to contain high amounts of vascular permeability-increasing activity as determined by a rat skin-Evans blue test. When the ascites was fractionated by gel filtration followed by reversed-phase high-performance liquid chromatography (HPLC) a component could be isolated which by its total sequence and amino acid composition was identified as Ile-Ser-bradykinin. Several degradation products of this kinin were also detected as separate components in the chromatographies. The human Ile-Ser-bradykinin appeared on reversed-phase HPLC in the same position as synthetic T-kinin. It could be differentiated in this chromatography system from Met-Lys-bradykinin, Lys-bradykinin and bradykinin. It may be assumed that human Ile-Ser-bradykinin is released_ from a third, so far unidentified human kininogen which is only or predominantly expressed under certain pathophysiological conditions, and that therefore this new kinin might be employed as a tumor marker.
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Tan, Soo Choon, Jit Kang Lim, Wen Yin Guan, Wei Yan Lee, and Mervyn Wing On Liew. "UV LED-based lightweight fixed-wavelength detector: for the development of a miniaturized high-performance liquid chromatography (HPLC) system (Erratum)." In Light-Emitting Diodes: Materials, Devices, and Applications for Solid State Lighting XXII, edited by Li-Wei Tu, Martin Strassburg, Jong Kyu Kim, and Michael R. Krames. SPIE, 2018. http://dx.doi.org/10.1117/12.2286230.

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Jie Tu, Fan Yang, Guanglin Li, and Liping Wang. "Analysis of interstitial concentrations of ATP from rat soleus muscle using microdialysis combined with ion-pairing high performance liquid chromatography (HPLC)." In 2009 Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 2009. http://dx.doi.org/10.1109/iembs.2009.5334425.

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Madar, Olivier, Emmanuelle Ledauphin, Keyvan Rezaï, Eve Camps, Catherine Tainturier, and Francois Lokiec. "Abstract 2451: Development of a high performance liquid chromatography (HPLC) method for controlling the radiochemical purity (RCP) of 99mTc-tetrofosmin used in oncology." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-2451.

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Reports on the topic "HPLC (high-performance liquid chromatography)"

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Dennis, William E., and Alan B. Rosencrance. Determination of N-Nitroso-N-Ethylurea (ENU) in Salt Water - By High Performance Liquid Chromatography (HPLC). Fort Belvoir, VA: Defense Technical Information Center, June 1995. http://dx.doi.org/10.21236/ada297217.

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Manning, D. L., and M. P. Maskarinec. Analysis of nitroguanidine in aqueous solutions by HPLC (high performance liquid chromatography) with electrochemical detection and voltammetry: Final report. Office of Scientific and Technical Information (OSTI), April 1987. http://dx.doi.org/10.2172/6669393.

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Stromer, Bobbi, Rebecca Crouch, Katrinka Wayne, Ashley Kimble, Jared Smith, and Anthony Bednar. Methods for simultaneous determination of 29 legacy and insensitive munition (IM) constituents in aqueous, soil-sediment, and tissue matrices by high-performance liquid chromatography (HPLC). Engineer Research and Development Center (U.S.), September 2021. http://dx.doi.org/10.21079/1168142105.

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Standard methods are in place for analysis of 17 legacy munitions compounds and one surrogate in water and soil matrices; however, several insensitive munition (IM) and degradation products are not part of these analytical procedures. This lack could lead to inaccurate determinations of munitions in environmental samples by either not measuring for IM compounds or using methods not designed for IM and other legacy compounds. This work seeks to continue expanding the list of target analytes currently included in the US Environmental Protection Agency (EPA) Method 8330B. This technical report presents three methods capable of detecting 29 legacy, IM, and degradation products in a single High Performance Liquid Chromatography (HPLC) method with either ultraviolet (UV)-visible absorbance detection or mass spectrometric detection. Procedures were developed from previously published works and include the addition of hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine (MNX); hexahydro-1,3-dinitroso-5-nitro-1,3,5-triazine (DNX); hexahydro-1,3,5-trinitroso-1,3,5-triazine (TNX); 2,4-diamino-6-nitrotoluene (2,4-DANT); and 2,6-diamino-4-nitrotoluene (2,6-DANT). One primary analytical method and two secondary (confirmation) methods were developed capable of detecting 29 analytes and two surrogates. Methods for high water concentrations (direct injection), low-level water concentrations (solid phase extraction), soil (solvent extraction), and tissue (solvent extraction) were tested for analyte recovery of the new compounds.
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Clifford, D. J., D. E. McKinney, Lei Hou, and P. G. Hatcher. Coal liquefaction process streams characterization and evaluation: High performance liquid chromatography (HPLC) of coal liquefaction process streams using normal-phase separation with uv diode array detection. Office of Scientific and Technical Information (OSTI), January 1994. http://dx.doi.org/10.2172/10143663.

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Maskarinec, M. P., D. L. Manning, and R. W. Harvey. Application of XAD-4 solid sorbent and HPLC (high performance liquid chromatography) with electrochemical detection to the analysis of phenols in water: Final report, November 1983-November 1985. Office of Scientific and Technical Information (OSTI), June 1987. http://dx.doi.org/10.2172/6478788.

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Crouch, Rebecca, Jared Smith, Bobbi Stromer, Christian Hubley, Samuel Beal, Guilherme Lotufo, Afrachanna Butler, et al. Preparative, extraction, and analytical methods for simultaneous determination of legacy and insensitive munition (IM) constituents in aqueous, soil or sediment, and tissue matrices. Engineer Research and Development Center (U.S.), August 2021. http://dx.doi.org/10.21079/11681/41480.

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No standard method exists for determining levels of insensitive munition (IM) compounds in environmental matrices. This project resulted in new methods of extraction, analytical separation and quantitation of 17 legacy and 7 IM compounds, daughter products of IM, and other munition compounds absent from USEPA Method 8330B. Extraction methods were developed for aqueous (direct-injection and solid-phase extraction [SPE]), soil, sediment, and tissue samples using laboratory-spiked samples. Aqueous methods were tested on 5 water sources, with 23 of 24 compounds recovered within DoD QSM Ver5.2 limits. New solvent extraction (SE) methods enabled recovery of all 24 compounds from 6 soils within QSM limits, and a majority of the 24 compounds were recovered at acceptable levels from 4 tissues types. A modified chromatographic treatment method removed analytical interferences from tissue extracts. Two orthogonal high-performance liquid chromatography-ultraviolet (HPLC-UV) separation methods, along with an HPLC–mass spectrometric (HPLC-MS) method, were developed. Implementing these new methods should reduce labor and supply costs by approximately 50%, requiring a single extraction and sample preparation, and 2 analyses rather than 4. These new methods will support environmental monitoring of IM and facilitate execution of risk-related studies to determine long-term effects of IM compounds.
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Crouch, Rebecca, Jared Smith, Bobbi Stromer, Christian Hubley, Samuel Beal, Guilherme Lotufo, Afrachanna Butler, et al. Methods for simultaneous determination of legacy and insensitive munition (IM) constituents in aqueous, soil/sediment, and tissue matrices. Engineer Research and Development Center (U.S.), August 2021. http://dx.doi.org/10.21079/11681/41720.

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Currently, no standard method exists for analyzing insensitive munition (IM) compounds in environmental matrices, with or without concurrent legacy munition compounds, resulting in potentially inaccurate determinations. The primary objective of this work was to develop new methods of extraction, pre-concentration, and analytical separation/quantitation of 17 legacy munition compounds along with several additional IM compounds, IM breakdown products, and other munition compounds that are not currently included in U.S. Environmental Protection Agency (EPA) Method 8330B. Analytical methods were developed to enable sensitive, simultaneous detection and quantitation of the 24 IM and legacy compounds, including two orthogonal high-performance liquid chromatography (HPLC) column separations with either ultraviolet (UV) or mass spectrometric (MS) detection. Procedures were developed for simultaneous extraction of all 24 analytes and two surrogates (1,2-dinitrobenzene, 1,2-DNB; o-NBA) from high- and low-level aqueous matrices and solid matrices, using acidification, solid phase extraction (SPE), or solvent extraction (SE), respectively. The majority of compounds were recovered from four tissue types within current limits for solids, with generally low recovery only for Tetryl (from 4 to 62%). A preparatory chromatographic interference removal procedure was adapted for tissue extracts, as various analytical interferences were observed for all studied tissue types.
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Sales, B. C., L. A. Boatner, B. C. Chakoumakos, J. C. McCallum, J. O. Ramey, and R. A. Zuhr. Structural analysis of amorphous phosphates using high performance liquid chromatography. Office of Scientific and Technical Information (OSTI), December 1993. http://dx.doi.org/10.2172/10111962.

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Bass, D. A., J. S. Yaeger, J. S. Crain, J. T. Kiely, K. J. Parish, M. J. Gowdy, and G. B. Mohrman. Arsenic speciation using high performance liquid chromatography-inductively coupled plasma-mass spectrometry. Office of Scientific and Technical Information (OSTI), August 1995. http://dx.doi.org/10.2172/102125.

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Dumont, Philip John. New methods and materials for solid phase extraction and high performance liquid chromatography. Office of Scientific and Technical Information (OSTI), April 1996. http://dx.doi.org/10.2172/219499.

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